Page No. 1/2
FC-PF-229
PESTICIDE FORMULATION & RESIDUE ANALYTICAL CENTRE, PMD, NIPHM, HYDERABAD
Sr. No. in Scope
NABL / NON NABL
Flow Chart for Analysis of 2, 4-D Sodium Salt
Date of Analysis
Sl.
No.
1.
2
2.1
3.
3.1
3.2
4.
4.1
Step
Sample No.
Name of Sample
Sample Description
Preparation of NaOH Standard solution (0.1 N):
Weigh 4 g of NaOH and dissolve in water to give 1 L
solution.
Note the Serial No. of Balance log book
4.2
Standardization of 0.1N NaOH Solution:
Weigh 0.42-0.45 g GR grade Potassium Hydrogen Phthalate
(KHP) in a 250 mL conical flask.
Note the Serial No. of Balance log book
4.3
Dissolve in 50-70 mL of water
4.4
Add Phenolphthalein indicator.
4.5
Titrate with 0.1N NaOH solution taken in a burette slowly
with continuous stirring from colourless to faint pink
colour.
Titre value for standardization is
4.6
5.
5.1
5.1.1
5.1.2
5.1.3
5.1.4
5.1.5
5.1.6
5.1.7
5.1.8
5.1.9
Executed
By
Execution
Procedure
Preparation of Sample Solution
Weigh Accurately about 1 g of Sample in a 100 mL beaker.
Note the Serial No. of balance log book.
Dissolve the Sample in about 10 mL distilled Water.
Transfer it quantitatively to a 250 mL separating funnel
using minimum quantity of water.
Acidify the solution with about 1.5 mL of hydrochloric acid
(1:1) using methyl orange indicator.
Extract the aqueous phase with three successive portions of
25 ml diethyl ether.
Combine ether extracts from all the three separating funnels
into one separating funnel, quantitatively.
Then wash the ether layer with distilled water (25 mL) until
the ether solution is free of chloride ions. (Test for complete
removal of chlorides with 0.1 N AgNO3).
Transfer the ether solution to a 250 ml beaker and
evaporate the ether on water bath to dryness.
R1
R2
g
g
g
g
mL
g
mL
g
Name of the Laboratory :
Pesticide Formulation & Residue Analytical Centre, PMD, NIPHM, Hyderabad
Flow Chart for analysis of 2,4-D Sodium
Document No.
: FC-PF-229
Document Name
:
Salt
Revision No.
: 02
Issue Date
:
11/07/2013
Revision Date
: 26/03/2014
Next Revision Date
:
26/03/2016
Prepared By
Checked By
Approved & Issued By
Mrs. T. Sridevi
(Assistant Scientific Officer)
Mr. C.V. Rao
(Technical Manager)
Dr Abhay Ekbote
(Director PM & Quality Manager)
Page No. 2/2
FC-PF-229
PESTICIDE FORMULATION & RESIDUE ANALYTICAL CENTRE, PMD, NIPHM, HYDERABAD
5.1.10 Dissolve the residue in 80 mL of neutral alcohol and titrate
with 0.1 N sodium hydroxide solution using bromo thymol
blue indicator to green colour Endpoint.
5.1.11 Titre value for sample is
mL
mL
6. Calculation:
Wt. of KHP X 1000
Normality of NaOH (N) = ---------------------------------------------------Volume of NaOH consumed X 204.22
V X N X 22.1
Acid (2,4-D) Content, = ----------------------percent by mass
M
V = Volume of NaOH required for sample
N = Normality of NaOH
M = Weight of sample taken for analysis
Result:
Sl. No.
Name of test
1.
Result
(2,4-D) Acid Content
Unit
Method of Analysis
%
IS 1488 : 1989
(Reaffirmed 2009)
Remark / Reference :
Name
Analyzed by
Dated signature
Name
Checked by
Dated signature
Name of the Laboratory :
Pesticide Formulation & Residue Analytical Centre, PMD, NIPHM, Hyderabad
Flow Chart for analysis of 2,4-D Sodium
Document No.
: FC-PF-229
Document Name
:
Salt
Revision No.
: 02
Issue Date
:
11/07/2013
Revision Date
: 26/03/2014
Next Revision Date
:
26/03/2016
Prepared By
Checked By
Approved & Issued By
Mrs. T. Sridevi
(Assistant Scientific Officer)
Mr. C.V. Rao
(Technical Manager)
Dr Abhay Ekbote
(Director PM & Quality Manager)