Topic 10: Mitosis and Karyotypes
 Know the stages of mitosis and what happens in each
 Be able to recognize these stages via pictures, slides, or models
 Be able to estimate the duration of a mitotic stage given the necessary data
 Be able to complete a karyotype.
 Explain the purpose of Giemsa stain.
 Know the appropriate terminology for mitosis including haploid and diploid, chromatid, chromosome, centromere, chromatin, cytokinesis, homologous.
Transformation Efficiency Practice Problem
We added 10  l of 0.005  g/  l pAMP plasmid to a tube of E. coli. The bacteria were suspended in 300  l of cold calcium chloride. After heat shock, 300  l of Luria broth was added to the tube. 150  l of cell suspension was placed on agar plates. The plates were incubated at 37 o
C for 24 hours. 35 colonies were counted on the +T plate.
What is the Transformation Efficiency?
Answer on next page.

A. 0.005
 g/
 l x 10
 l .05
 g pAMP
B. 150
 l 0.25
600
 l
C. 0.25 x 0.05
 g pAMP 0.0125
 g pAMP
D. 35 colonies
0.0125
 g pAMP
2.8 X 10 3 colonies/
 g pAMP