Disease Control Products for Stored Potatoes Jill Thomson and Doug Waterer Dept of Plant Sciences, University of Saskatchewan Control of Storage Diseases • Challenge - need dependable control of various diseases during storage/handling • Challenge - need safe, affordable products • Challenge - products must be readily applied with available equipment Control of Storage Diseases • Traditional options – Mertec (TBZ) • • • • • spray applied during load-in good coverage critical not effective against bacteria not effective against Late Blight extensive resistance in Dry Rot and Silver scurf • $ 6.40/tonne at CDN label rate Control of Storage Diseases • Available options – Dithane (Mancozeb) • • • • spray applied during load-in good coverage critical not effective against bacteria effective against Dry Rot and Silver scurf • $ 11.06/tonne • only registered for use on seed Control of Storage Diseases • Alternate options – Purogene – Oxidate – Ozone Control of Storage Diseases • Purogene (Anthium) – UAP – Sodium chlorite + acid = Chlorine dioxide (gas) + other Cl species – applied as spray to potatoes going into storage and/or – added to ventilation air Control of Storage Diseases • Purogene – works by oxidation reactions – label indicates effective against L. blight, Silver Scurf, Dry Rot and Bacterial Soft rot – inactivated by soil – no residual activity – repeated or continuous treatment recommended Control of Storage Diseases • Purogene - Status – Researched by MSU, NDSU, Idaho, MB and AB. – 5th year of Class 18 Exemption in U.S. – Emergency use in Canada in 1999, 2000 and 2001 but not in 2002 Control of Storage Diseases • Purogene Efficacy against Soft rot – MB data – some control Soft Rot Control with Purogene 100 80 % 60 Soft 40 Rot 20 0 0 50 100 400 PPM Purogene Russets Shepody 1 Shepody 2 Control of Storage Diseases Effect of Purogene on the amount of rot found on tubers (MANITOBA) Cultivar Russet B. Shepody Shepody Concentration Avg Rot per Tuber (%) 0 23.5 50 3.8 200 1.1 400 2.2 0 9.1 50 5.8 400 3.2 0 1.1 50 2.4 200 0.2 400 0.3 Control of Storage Diseases • Purogene vs Late Blight – MB data – Tubers treated immediately after inoculation – some control Control Purogene 100 80 60 40 20 0 Trial 1 Trial 2 Trial 3 Trial 4 Control of Storage Diseases • Purogene vs Rhizoctonia, Silver Scurf, Soft Rot and Dry Rot – – – – AB data Tubers sprayed at load in Tubers treated in storage (weekly) minimal control Control of Storage Diseases • Purogene - Safety – mixing step requires safety equipment (ClO2 vapors) – ventilate storage for 2 h prior to re-entry – potatoes must be washed prior to consumption – slowly corrodes copper, brass, aluminum Control of Storage Diseases • Purogene - Economics – 16-32 ml/ton applied at load-in – max rate of 16 ml/ton/month applied during storage humidifaction – $25/L – $ 2.50/ton for pre-treatment + continuous application over 5 months Control of Storage Diseases • Purogene Conclusions – variable results – problems getting full activation – problems with inactivation by soil – problems with uniformity of distribution of effective dosages – problems with adding humidity to problem storages – relatively safe and affordable Control of Storage Diseases • Oxidate – BioSafe – Hydrogen Peroxide + acetic acid – applied as spray to potatoes going into storage and/or – added to ventilation air Control of Storage Diseases • Oxidate – works by oxidation reactions – label indicates effective against L. blight, Silver Scurf, Dry Rot and Bacterial Soft rot – inactivated by soil – no residual activity – continuous treatment recommended Control of Storage Diseases • Oxidate - Status – approved in U.S. for use in wide range crops in field and storage – approved as an “organic” pesticide – “Emergency Use” in BC and AB in 2002 – full registration anticipated for April 2003 – NB has done some testing in potatoes Control of Storage Diseases • Oxidate - Efficacy Control of Storage Diseases • Oxidate - Efficacy FDR development after Oxidate treatment for 16 weeks Average % dry rot 25 20 15 Shepody Norland 10 5 0 0 1% 2% Oxidate treatment Control of Storage Diseases • Oxidate - Efficacy Incidence silver scurf (av.%) Silver scurf development after Oxidate treatment for 16 weeks 100 80 60 Shepody Norland 40 20 0 0 1% 2% Oxidate treatment Control of Storage Diseases • Oxidate - Efficacy Soft rot development after Oxidate treatment for 16 weeks Soft rot (%) 5 4 3 2 Shepody Norland 1 0 0 1% 2% Oxidate treatment Control of Storage Diseases • Oxidate - Safety – no mixing step – concentrated product is corrosive – no re-entry restriction – no limit to amount applied – no requirement to wash prior to consumption – slowly corrodes copper, brass, aluminum Control of Storage Diseases • Oxidate - Economics – 1-2% solution applied to wetness at load-in – 1:100 to 1:300 applied during storage humidifaction – $17.78/L – pre-treatment = $ 0.71-1.42/tonne – 1% in humidification system = $1.78 per injection – How many injections into system over 5 month period? Control of Storage Diseases • Oxidate – Conclusions – potential to reduce disease levels – high dosages and continuous application most effective – safe and affordable – need uniform delivery of effective dosages – adding humidity to problem potatoes ? – Impact on sprouting ?? Control of Storage Diseases • Ozone – various manufacturers – O2 + high energy = O3 (gas) – applied at high levels to potatoes going into storage and/or – added to ventilation air Control of Storage Diseases • Ozone – works by oxidation reactions – lab studies indicates effective against range of bacteria and fungi – inactivated by soil – no residual activity – continuous treatment recommended Control of Storage Diseases • Generator unit, outside storage Control of Storage Diseases • Monitoring equipment mounted above conveyor. Injection of O3 into conveyor system, removal of excess O3. Control of Storage Diseases • Ozone - Status – well established in other food areas – data for storage use is limited – advantage = does not depend on water for delivery (spray or humidity) Control of Storage Diseases • Ozone – Efficacy % Incidence FDR on surface-inoculated tubers 80 70 60 50 40 30 20 10 0 OZONE Control 1 day 1 wk 3 wks Exposure time Control of Storage Diseases • Ozone – Efficacy % Incidence Silver scurf after ozone treatment 30 25 20 15 10 5 0 Ozone Control 1 day 1 wk 3 wks Exposure time Control of Storage Diseases • Ozone – Efficacy – O3 applied loading into storage 20 15 10 Norland Yukon Gold 5 O zo ne M er te c D ith an e on tr ol 0 C Extent of FDR damage (sq.cm) FDR development in wounded, inoculated tubers Control of Storage Diseases • Ozone - Safety – widely used to sanitize food and buildings – corrosive at high concentrations – exposure levels well established – no limit to amount applied – no requirement to wash prior to consumption Control of Storage Diseases • Ozone - Economics – generators + power – 500 ppm during loading – 2 ppm during storage ventilation –$? Control of Storage Diseases • Ozone - Conclusions – primarily provides contact control – most effective against surface problems (scurf and bacteria) – ineffective against aggressive or well established infections – economics ? – Impact on product/facilities ? Control of Storage Diseases • Conclusions – Purogene and Oxidate demonstrated to provide some control under certain conditions – Ozone also shows potential – Most effective against new, surface problems – Supplement to good harvest and storage management practices Control of Storage Diseases • In the future ? – No new chemical products in the pipeline – BioCide is developing granular products for time-release uses – Help overcome short reaction times of chlorine dioxide and improve in-storage distribution – Further out … Bacterial antagonists to common diseases