JESSICA BRUNQUELL
Department of Cell Biology, Microbiology, and Molecular Biology| University of South Florida| 4202 East
Fowler Ave, ISA 2015 Tampa, FL 33620 Lab Phone: (813) 974-5538| Email: jlhoskin@mail.usf.edu
PROFESSIONAL EDUCATION
Pasco-Hernando Community College
Associates in Arts Degree
Biology Focus
2007-2010
University of South Florida
B.S. Biology
Focus in Cell Biology, Microbiology, and Molecular Biology
2010-2012
University of South Florida
Cell Biology, Microbiology, and Molecular Biology Department
Thesis Masters
PI: Sandy Westerheide
Project: Genetic Modulators of HSF-1 Regulation in Caenorhabditis elegans
2013 - Present
AWARDS
Tharp Fellowship
Tuition Stipend
Summer 2013
2013 - Present
TEACHING EXPERIENCE
Biology of Humans
Genetics Laboratory
Spring 2013, Fall 2013, Spring 2014
Summer 2013
PUBLICATIONS AND PAPERS
AROS and DBC1 Modulate the Heat Shock Response in a SIRT1-dependent Manner. Raynes R, Pombier K,
Nguyen K, Brunquell J, Mendez J, Westerheide SD. PLOS One, 2013.
Stress Inducibility of SIRT1 and Its Role in Cytoprotection and Cancer. Rachel Raynes, Jessica
Brunquell, and Sandy D. Westerheide. Genes & Cancer, 2013.
Negative Regulation of the HSR in C. elegans by LST-3, a DBC1 Ortholog.
Raynes, Philip Bowers, and Sandy D. Westerheide. [In Prep]
Jessica Brunquell, Rachel
Evolution and disorder analyses show that DBC1/CCAR2 and CCAR1 have evolved from the nematode
protein LST-3. Jessica Brunquell, Sandy D. Westerheide, Bin Xue. [In Prep]
5-fluoro-2’-deoxyuridine increases chaperone expression and decreases polyglutamine aggregation in an
HSF-1-dependent manner in Caenorhabditis elegans. Jessica Brunquell, Philip Bowers, Sandy D.
Westerheide. [In Prep]
JESSICA BRUNQUELL
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POSTER PRESENTATIONS
18th Annual Midwest Stress Meeting, Northwestern University
(Evanston, IL)
January 2013
5th Annual University of South Florida Graduate School ResearchONE
(Tampa, FL)
March 2013
Directed undergraduate research poster presentations for the 2013 University of South Florida
Undergraduate Research and Arts Colloquium
May 2013
(Tampa, FL)
Oktoberfest at the University of South Florida
(Tampa, FL)
October 2013
19th Annual Midwest Stress Meeting, Northwestern University
(Evanston, IL)
January 2014
6th Annual University of South Florida Graduate School ResearchONE
(Tampa, FL)
March 2014
Undergraduate research poster presentations for the 2014 University of South Florida
Undergraduate Research and Arts Colloquium
May 2014
MENTORING, COMMUNITY OUTREACH, AND TECHNICAL COURSES
Volunteered to assist 6 high school students of under-represented minorities
Assisted students in laboratory techniques 8 hours a week, consisting of C. elegans
maintenance, cloning, and gel electrophoresis.
Directed Undergraduate Honor’s Thesis Project
Student: Philip Bowers
Thesis: The Oxidative Stress Response and the Heat Shock Response in C. elegans
Volunteered for the STEM Academy Program
Assisted high school students in a genetics lab experiment consisting of gel
electrophoresis, and a DNA extraction.
Summer2012
Spring 2013-Present
Summer 2013
Attended an Intensive C. elegans Course at the University of Florida Directed by Dr. Keith Choe
Learned identification of C. elegans major tissues, making males/mating,
microinjection to produce chromosomal arrays, gene integration, mutagenesis screening,
and worm sorting techniques
Spring 2014
JESSICA BRUNQUELL
Directed Undergraduate Independent Research
Alexander Quattlebaum
Chelsea Phillips
Philip Bowers
Stephanie Morris
Alana Snyder
Mark Noble
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Fall 2012-Spring 2013
Spring 2013 – Summer 2014
Fall 2012 - Present
Spring 2013 - Present
Fall 2013 – Present
Spring 2014-Present
TECHNICAL LABORATORY EXPERIENCE
Extensive knowledge of C. elegans care, breeding, and maintenance
Cell and tissue culture
C. elegans phenotypic assays (i.e. cytoprotection, thrashing, paralysis)
DNA, RNA, and protein extraction and purification
Primer Design
PCR, RT-PCR, qRT-PCR
E. coli transformation
OTHER RELEVANT LABORATORY EXPERIENCE
Ordering and inventory of supplies and reagents
Mentoring undergraduate students
Managing entry rotation graduate students
SDS-Page and Western blotting
Chromatin Immunoprecipitation
in C. elegans
C. elegans RNAi knockdown
Luciferase assays
Transfection
Gel electrophoresis