General Biology – Unit 2 Review Mary Stangler Center for Academic Success This review is meant to highlight basic concepts from unit 2 (cells, membranes, energy/enzymes). It does not cover all concepts presented by your instructor. Refer back to your notes, unit objectives, labs, handouts, etc. to further prepare for your exam. A: Cell Theory and Prokaryotic Cells Lesson 1. What are the 3 components of the cell theory? 2. Name the scientists who contributed to the development of the cell theory. 3. Make a table below in order to classify the following criteria with the correct basic cell type, either prokaryote or eukaryote. All have a cell wall, Only plants/fungi/some protists have cell wall, Membrane bound organelles, No membrane bound organelles, No nucleus, Nucleus, Simple cells, Specialized cells, Unicellular, Unicellular/multicellular Prokaryote eukaryote 4. The following is an example of an organism, is it prokaryote or eukaryote? A unicellular organism has a flagellum and peptidoglycan in the cell wall. 5. What do prokaryotes have in place of a true nucleus? 6. Even though prokaryotes do not have membrane bound organelles they do have this important organelle for synthesizing proteins. ________________________________ 7. Explain the function of each cell part for a bacterial cell. Capsule Cell wall Cell membrane Cytoplasm Ribosome Nucleoid Pili Flagella – Rev. 2.27.2013 pg. 1 8. If a bacterium has a thick peptidoglycan wall will it stain purple or red when using a Gram stain technique? Is this considered Gram positive or Gram negative? 9. What are the 3 main shapes of prokaryotic cells? B: Eukaryotic Cells Lesson 10. Describe the structure and function for each part of the eukaryotic cell. Cell membrane Nucleus Nucleolus Ribosome Smooth endoplasmic reticulum Rough endoplasmic reticulum Golgi apparatus Lysosome Peroxisome Vesicle Mitochondria Chloroplasts Cytoskeleton Microtubule Actin filaments Intermediate filament Cell wall Nuclear envelope Vacuole Rev. 2.27.2013 pg. 2 11. Label the parts of a plant cell (A) and animal cell (B) . A: Plant Cell B: Animal Cell: Rev. 2.27.2013 pg. 3 12. Make a table below in order to classify the following criteria with the correct kingdom of eukaryotes, either animal or plants. Cell wall, Cellulose, Centrioles, Chloroplasts, No cell wall, No cellulose, No centrioles, No chloroplasts, Small or no vacuole, Vacuole Animal Plant C: Cell Membrane Lesson 13. Name the basic components that make up a cell membrane. 14. Define the components of a phospholipid (chapter 3). 15. Discuss the difference between hydrophilic and hydrophobic and how these terms relate to how a phospholipid bilayer forms to make a cell membrane. 16. Discuss the following functions of membrane proteins. Channel proteins Carrier proteins Cell recognition proteins Receptor protein Enzymatic protein Junction proteins 17. Explain the difference between integral and peripheral membrane proteins. 18. Why is membrane transport important to a cell? 19. What is the one major difference between passive and active transport? Rev. 2.27.2013 pg. 4 20. What is meant by a concentration gradient? 21. Make a table below in order to classify the following forms of membrane transport as either passive or active transport. Bulk transport, Carrier proteins, Diffusion, Endocytosis, Exocytosis, Facilitated diffusion, Phagocytosis, pinocytosis, Protein pumps Passive transport Active transport 22. Which general form of transport causes a net movement of molecules from a low concentration to a high concentration (uphill)? 23. Define Isotonic – Hypertonic – Hypotonic – If a cell shrinks (crenates) when placed in a solution what can be said about the tonicity of the cell and the solution? Explain 24. Match the following scenarios with the correct transport system. a. 3 sodium ions move from an area of low concentration across the membrane to an area of high concentration. b. A special form of diffusion that only involves water. c. Oxygen molecules pass from an area of high concentration through cell membranes in the lung to reach the bloodstream where oxygen concentration is low d. Process by which the cell membrane envelops food/liquid to bring it into the cell. e. Process by which vesicles carrying waste fuse with the cell membrane to remove it from the cell. f. Sodium can’t easily pass the bilayer so they flow from an area of high concentration in the extracellular fluid through a channel to an area of lower concentration in the cytoplasm ______ Diffusion ______ Facilitated diffusion ______ Osmosis ______ Protein pump/carrier protein ______ Endocytosis ______ Exocytosis Rev. 2.27.2013 pg. 5 25. A container has a semi-permeable membrane dividing it into 2 compartments. 10 mL of a 10% NaCl solution is poured into side A and 10 mL of a 20% NaCl solution is poured into side B. Compare the volumes of each side if left to rest for a period of time. Will the volumes on each stay the same or change? Why does this happen? D: Energy and Enzymes Lesson 26. Define the 1st and 2nd Laws of Thermodynamics and give an example of how each relates to living systems. 27. Describe the difference between an endergonic and exergonic reaction. 28. Define kinetic and potential energy and how they relate to the 1 st Law of Thermodynamics. 29. What is the term for “extra” energy needed to get a chemical reaction started? 30. What substances enhance chemical reactions by lowering activation energy? 31. When chemical bonds are broken, energy is released, is this an exergonic or endergonic reaction? 32. When chemical bonds are built, energy is required, is this an exergonic or endergonic reaction? 33. Define substrate and active site. Describe how they interact to catalyze reactions. 34. Describe the structure and function of ATP. 35. Describe the ATP cycle. Rev. 2.27.2013 pg. 6