Biochemistry

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BIOCHEMISTRY

The chemistry of life

ORGANIC COMPOUND

Contains CARBON and HYDROGEN

Ex. C

6

H

12

O

6 is GLUCOSE

INORGANIC COMPOUND

Does NOT contain carbon and hydrogen together.

Examples

H

2

O = water

CO

2

= carbon dioxide

ATOMS

One of the simplest units of matter

Made of:

Protons -have a positive charge (+)

Electrons -have a negative charge (-)

Neutrons -have NO charge (0)

ELEMENT

Made of one kind of atom

Examples:

C = Carbon

H = Hydrogen

O = Oxygen

N = Nitrogen

COMPOUND

Two or more atoms are chemically combined and held together by bonds .

FOUR GROUPS OF ORGANIC

MOLECULES

Carbohydrates

Proteins

Lipids

Nucleic Acids

All organic molecules are made of C,H,O,N

Examples

Bread

Candy

Brownies

Pasta

Rice

Beans

CARBOHYDRATES

All carbohydrates are made of sugars. (C,H,O)

CARBOHYDRATES

3 different types of carbohydrates

Monosaccharide

Disaccharides

Polysaccharides

CARBOHYDRATES

Monosaccharides

Made of one sugar

End in –ose

Source of energy

Has chemical formula C

6

Ratio of H to O is 2:1

H

12

O

6

Examples:

Glucose

Fructose

Maltose

Lactose

CARBOHYDRATES

Disaccharide

End in –ose

Ratio of H to O is 2:1

Consist of 2 sugar molecules

Example:

 sucrose

CARBOHYDRATES

Polysaccharides

3 or more sugars combined

Complex structure

Examples

Cellulose

Starches

HOW ARE MOLECULES COMBINED?

Dehydration Synthesis

The process by which molecules are joined together by removing water.

HOW ARE COMPOUNDS BROKEN DOWN?

Hydrolysis

The process by which compounds are separated from each other by adding water.

PROTEINS

Made of Amino Acids

Always contain C,H,O,

N

All structures in an organism are made of proteins.

Proteins make up:

Enzymes

Muscle Tissue

Blood Cells

Cell Growth and Repair

Hormones

PROTEINS

Amino Acids are bonded together with peptide bonds.

3 Different types

Monopeptide (1 Amino Acid)

Dipeptide (2 Amino Acids)

Polypeptide (3 Amino Acids)

AMINO ACID STRUCTURE

Amino Acids are made of 3 parts:

An amino group

A carboxyl group

An R side chain

Amino Acids

There are 20 types of amino acids

8 of them are essential

Ex. Tryptophan, Alanine, Arginine, Proline,

Serine

DEHYDRATION SYNTHESIS OF 2

AMINO ACIDS

HYDROLYSIS OF A PROTEIN

LIPIDS

Also called fats, oils and waxes

Organic (made of C, H, O)

No ratio of H to O

Found in all living things

Used for

 energy storage source of energy

 insulation

Protection sub-structure of cell membrane

LIPIDS

Lipids are made of two parts:

Glycerol

3 Fatty Acids

LIPIDS

Two types of fatty acids

Saturated

Fats that are NOT double bonded and are solid at room temperature

Ex. Butter, bacon grease, Crisco, cheese

Unsaturated

Fats that are double bonded.

Ex. Olive oil, Canola Oil

LIPIDS

In a typical lipid molecule, there are carboxyl groups

NUCLEIC ACIDS

DNA (deoxyribonucleic acid)

Contains genetic information (heredity)

Controls cellular activities

Found in all living things

It is organic (C, H, O, N, P)

NUCLEIC ACIDS

DNA is made of units called nucleotides

Nucleotides consist of:

A phosphate group

A monosaccharide (ribose)

A nitrogenous base (A, T, G, or C)

A NUCLEOTIDE

DNA

Double helix

Bases:

Adenine (A)

Thymine (T)

Guanine (G)

Cytosine (C)

DNA bases are bonded using hydrogen bonds

RNA

Carries genetic information from the nucleus to the ribosomes, for protein synthesis

Single stranded (NOT double helix)

Smaller than DNA

RNA

Made of nucleotides

Contains sugar, phosphate group and 1 base

Bases in RNA:

Adenine (A)

Uracil (U)

Guanine (G)

Cytosine (C)

ENZYMES

Made of proteins

Contain: ____ _____ _____ _____

Enzymes are organic catalysts

Catalysts are chemicals that help chemical reactions occur

Enzymes remain the same during a chemical reaction

Always end in –ase

Ex. Lipase, Protease, Glucase, Lactase

ENZYMES

Lock-and-Key Model

ENZYMES

In the Lock and Key Model, enzymes must fit their substrate (enzymes are “ specific ” )

ENZYMES

Each enzyme can work on only one specific substrate

Ex.

Lipase works only on __________

Sucrase works only on ____________

Protease works only on ____________

__________ works only on fructose

ENZYMES

Enzymes help perform dehydration synthesis

ENZYMES

Enzymes help perform hydrolysis

ENZYMES

Re-draw and label each part of the picture

ENZYMES

Enzymes are effected by the following factors:

Temperature pH

Concentration of enzyme

Concentration of substrate

ENZYMES

Temperature

Enzymes work best at an optimum temperature

Optimum temperature for human enzymes is

______, C or _________, F

Enzyme activity is slower as temperature gets too cold or too hot

Temperature

ENZYMES

ENZYMES

Temperature

At high temperatures, enzymes will lose their shape

They denature

Misshapen enzymes no longer fit in the Lock and

Key Model, so chemical reactions do not take place

ENZYMES

 pH

Enzymes rate of reaction is effected by the amount of acid or base in an environment

 pH

ENZYMES

ENZYMES

Optimum pH for two different enzymes

ENZYMES

Concentration

Increasing the concentration (amount) of an enzyme or substrate, will only increase the rate of reaction, to a point

ENZYMES

Co-enzymes

Enzymes work with co-enzymes to speed up the rate of reactions.

Ex. vitamins

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