Exercise C: Logistic growth rates The biotic potential of a population is held in check by some form of “environmental resistance”. This might include limits on food, water or habitat, or waste accumulations. In most cases, populations tend to level off at a certain size, which is known as the carrying capacity of the environment. This population size is characteristic of the environment in which the organism lives and will vary among populations and among environments. Procedure: You can simulate the effects of environmental resistance on population growth with some simple equipment. Use a paper with a marked grid, a pair of dice, and a container of seeds. The squares on the grid represent an “environment” and the seeds are “organisms.” In this simulation, each organism is unable to move and occupies a unit of the environment indicated by the quadrants on the marked grid. Begin the simulation with a population size of 6. Roll the dice six times to place the organisms at random locations in the pan. (For example, if you roll a “3” and a “6”, place an “organism” in the sixth square of the third row--marked “36”.) As an alternative to rolling two dice, you can use a table of random numbers. Follow the instructions given to assign “organisms” to quadrants. Only one “organism” may occupy a quadrant. Follow the rules indicated here to simulate logistic growth: 1. Every individual alive at the start of a generation will produce one offspring during that generation. 2. The new individuals of each generation are placed in quadrants by dice or reference to a table of random numbers (see blue sheet) 3. First place 6 organisms. If random assignment places more than one organism in a quadrant, continue placing additional organisms until you have occupied 6 grid spaces. 4. These 6 (or more) organisms will all reproduce, each producing one offspring. Leave the original first generation organisms in their place. Count out the number of offspring produced and place in a pile off to the side of your workspace. 5. Roll the die, or use the random number table to assign these 6 (or more) new offspring to a grid space. Keep going until you have 12 spaces occupied with at least 1 organism. You may need to place additional offspring to get to 12 occupied grid squares. 4. Then apply the following rule: At the end of a generation (beginning with the end of generation 2), if more than one organism are present in a quadrant, all individuals die of starvation/resource depletion and are removed from that quadrant. 5. Record your observations for each generation in a table in your lab notebook similar to the one below. Generation # at start # after # of generation Reproduction 1 2 3 4 5 6 7 8 9 10 11 12 6 ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ 12 or ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ Loss due to Environmental Resistance __0_ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ # for Next generation 12 or ___ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ Analysis: After you complete the exercise, plot a graph in your lab notebook. Use generation number along the horizontal axis, and population size on the vertical axis. Plot one line showing “# at start of generation” (use solid circles for each data point). Then plot another line showing the population size for the first 5 generations if the population were to realize its biotic potential (i.e. 6, 12, 24, and 48, 96, etc.) (use open circles for biotic potential). Answer the following questions in your lab notebook using complete sentences. 1. Does the population grow at an exponential rate at any point in this curve? 2. What is the carrying capacity for the “environment” in this exercise? 3. What does the difference between biotic potential for this population and the carrying capacity of this environment represent? 4. What sorts of things might provide “environmental resistance” to exponential growth in natural populations?