Growth Response of Plants Prepared by Ms W.S.Kwan Plants have no nervous system. Can plants detect stimulus? Can plants response? A directional growth movement made by a part of a stationary plant in response to unilateral stimulus Types of Tropism stimulus gravity unilateral light water tropism geotropism phototropism hydrotropism Geotropism • Geotropism is the growth movement (growth response) shown by a plant part in response to gravity To show Geotropism in Seedlings To show Geotropism in Seedlings Why is it necessary to leave the apparatus in darkness ? Ans: To avoid any stimulus from light. To show Geotropism in Seedlings What has happened to the radicles and plumules ? Ans: The radicles grow downwards, while the plumules grow upwards. To show Geotropism in Seedlings Do the radicles show positive geotropism (towards gravity) or negative geotropism (away from gravity)? Ans: Positive geotropism. What advantage do the seedlings have by showing such geotropism ? Ans: Roots grow downwards deep into the soil to provide a firm anchorage underground and absorb water and mineral salts in the soil. Importance of Geotropism (Roots grow towards gravity) provide firm anchorage for roots ensure the plant can get adequate supply of water & mineral salts Clinostat for control experiments contains a cork disc which can be set to rotate in a vertical or horizontal plane by a motor Cancel out the effect of unilateral stimulus to make factors uniform (evenly distributed) To show the effect of Gravity by using a Clinostat plastic cover lined with moist blotting paper X in complete darkness Y What is the purpose of marking the radicles at equal intervals ? Ans: To find out which region of the radicle grows faster. plastic cover lined with moist blotting paper seedling in X X in complete darkness seedling in Y Y Does the direction of growth of the seedlings in clinostat X change ? Ans: The radicle of the seedlings in clinostat X bends downwards in response to the pull of gravity. plastic cover lined with moist blotting paper seedling in X X in complete darkness Y seedling in Y Does the direction of growth of the seedlings in clinostat Y change ? Why ? Ans: Since every side of the radicle of the seedling in clinostat Y is acted on by gravity, the radicle will grow straight horizontally. plastic cover lined with moist blotting paper seedling in X X in complete darkness seedling in Y Y What is the purpose of setting up clinostat Y ? Ans: Serve as a control for comparison. Shoots show negatively geotropic Phototropism • Phototropism is the growth movement (growth response) shown by a plant part in response to light. To show Phototropism in Shoots A unilateral light B C unilateral light light-proof box clinostat in complete darkness What is the effect of the clinostat on the shoot of plant B ? Ans: The revolving clinostat cancel out the effect of unilateral stimulus (light) on the shoot of plant B. A unilateral light B C unilateral light light-proof box clinostat in complete darkness What has happened to the shoots of plants A and B ? Ans: The shoots in pot A respond by growing towards the light source. The shoot in pot B do not show any curvature but grow vertically upwards. A unilateral light B C unilateral light light-proof box clinostat in complete darkness What has happened to the shoot of plant C ? Ans: the shoots in pot C grow vertically upwards with slender and longer nodes(elongated), and carries smaller leaves which are yellow in colour (etiolated). A unilateral light B C unilateral light light-proof box clinostat in complete darkness What is the significance of phototropism to plants ? Ans: This tropism ensures that a plant gets enough light energy for photosynthesis. Importance of Phototropism (Shoots grow towards light source) enables leaves to be in a position to receive maximum sunlight for photosynthesis Hydrotropism • Hydrotropism is the growth movement (growth response) shown by a plant part in response to water. Importance of Hydrotropism (Roots grow towards water supply) ensures that a plant gets enough water as a raw material for photosynthesis & enough mineral salts to maintain normal growth Types of Tropism stimulus gravity unilateral light water tropism geotropism phototropism hydrotropism shoot negative positive no response root positive negative positive response Mechanism Darwin Experiments Experiments on Oat Coleoptiles cut & replaced tip dark box few days later decapitated no growth Conclusions : The tip is responsible for the growth grows Experiments on Oat Coleoptiles unilateral light few days later black covers Conclusion : The tips are sensitive to light. (responsible for the detection of light) Experiments on Oat Coleoptiles dark box agar plate few days later Conclusions : The tip must have produced a diffusible chemical substance which stimulates growth Experiments on Oat Coleoptiles bending directions bending directions mica plate Can the explanation still work ? the tip is put aside Explanation of results : The side with the tip has a higher concentration of that particular chemical, the growth rate is faster than the other side. Auxins commonest plant hormones produced by shoot tip regulate growth & other physiological processes in plants promote growth by inducing cell elongation & cell division. Effect of auxin on Plant Parts positive growth shoot Growth of plant negative growth low conc. plant growth auxin concentration high conc. root auxin concentration high conc. shoot Positive (promote) root Negative (inhibit) low conc. no effect Positive (promote) Phototropism in Shoot The shoot tip produces auxins which diffuse downward The auxins diffuse to the shaded side due to the presence of unilateral light High auxin concentration stimulates growth in shoot The shaded side grows faster than the lighted side The shoot bends towards the light Geotropism in Root The auxins diffuse to the lower side due to gravity High auxin concentration inhibits growth in root The lower side of root grows slower than the upper side The root bends downward ~END~