Review • Compare and Contrast RNA vs. DNA. • What is the significant difference between Prokaryotes and Eukaryotes? • What are the three types of RNA? DNA • Sugar? • Nitrogen bases? • Double stranded? RNA ribonucleic acid • Single stranded • Contain “ribose sugar” • Small enough to leave the nucleus • 3 types of RNA – mRNA, tRNA and rRNA • Nitrogen bases: – A, G, C, Uracil Protein Synthesis • Process where DNA encodes for the production of amino acids • What does the body do with the proteins after they are made ? Protein diagrams Two major Steps • Transcription – Information is coded from DNA to make mRNA • Translation – Information coded from mRNA is used to make proteins Transcription Helpers • Helicase: “unzipper” • RNA poly: adds RNA nucleotides Transcription • mRNA is made from DNA • Initiation, Elongation, Termination • http://www.stolaf.edu/ people/giannini/biolog ical%20anamations.ht ml Steps of Transription 1. 2. 3. 4. 5. Helicase unwinds the DNA RNA poly binds to the promotor site RNA poly adds RNA nucleotides RNA reaches stop codon mRNA detaches and moves to cytoplasm (or stays in cytoplasm of prok) Translation • Process by which proteins are “translated” coded from mRNA • Takes place in Cytoplasm • ID the three types of RNA in the diagram. Translation • mRNA contains a message that must be translated • mRNA contains codons (3 nucleotide sequence.) • Each codon corresponds to an amino acid Translation • tRNA contains an anti-codon and an amino acid • One specific AA can correspond to more than one codon (degenerate) Steps of Translation 1. rRNA subunit attaches to the mRNA strand at the start codon: AUG 2. tRNA carries an anti-codon and a protein that corresponds to the genetic code 3. Peptide bonds form between the amino acids. Once the tRNA reaches a stop codon, a release factor binds and releases the protein into the cytoplasm where it is used as directed by the cell. Initiation, elongation, termination How it works • http://www.stolaf.edu/people/giannini/biolo gical%20anamations.html • http://highered.mcgrawhill.com/sites/0072437316/student_view0/ chapter15/animations.html# mRNA: AUG CCG AUC AUG UAA Protein Synthesis animations • http://www.stolaf.edu/people/giannini/biolo gical%20anamations.html • http://highered.mcgrawhill.com/sites/0072437316/student_view0/ chapter15/animations.html#