Purpose
• I would like to test the effects of energy
drinks on the heart rate of Daphnia
Magna.
Real Life Connection
• Teens are drinking a lot of energy drinks
these days. Some people have died from
them. I am trying to find the dangers of
energy drinks in my experiment.
Research
• There were studies at the University of
Miami Concerning the use of energy
drinks. The lead pediatrician was Dr.
Steven E. Lipshultz. According to studies
30-50% of adolescents are drinking
energy drinks regularly. The energy drink
market was supposed to have reached
over 9 billion dollars last year.
Research (continued)
• In many news reports it has cautioned
parents against energy drinks due to the
amount of caffeine in these drinks. The
healthy daily limit for a twelve year old is
about 100mg every day. Most energy drink
companies claim that they are dietary
supplements and, therefore, are not
monitored by the Food and Drug
Association (FDA) like soft drinks.
Research (continued)
Drink
Monster
Mg of Caffeine
per fl. Ounce
10.0mg
Amp
8.875mg
Red Bull
9.524mg
Nos
16.25mg
5-Hour
71.502
Research (continued)
• Daphnia Magna
Diagram
http://resources.wardsci.com/wp-content/themes/obscure/library/images/daphniadiagram.png
Bibliography
• Lipshulz, Dr. Steven E. "New Report Shows Energy Drinks May
Harm Children - ABC News." ABCNews.com - Breaking News,
Latest News & Top Video News - ABC News. American
Broadcasting Company, 2012. Web. 27 Dec. 2011.
<http://abcnews.go.com/Health/report-shows-energy-drinks-harmchildren/story?id=12901333>.
• N.A. "Caffeine Content of Drinks." Energy Fiend - The Caffeine Fix.
Energy Freind, 2012. Web. 28 Dec. 2011.
<http://www.energyfiend.com/the-caffeine-database>.
• N.A. "Working with Daphnia » Ward's Science Teacher
ResourcesWard's Science Teacher Resources." Home - Ward's
Science Teacher ResourcesWard's Science Teacher Resources.
Ward's Natural Science. Web. 1 Jan. 2012.
<http://resources.wardsci.com/livecare/working-with-daphnia/>.
Hypothesis
• I think the 5 Hour energy drink will have
the most effect on the Daphnia Magna in
quantity and singularly because of the
amount of mg of Caffeine per fl. oz.
Materials
•
•
•
•
•
•
•
•
6 pipettes
A sharpie
A microscope
15 microscope slides
One can of Amp
One can of Monster
One can of Nos
One can of Red Bull
•
•
•
•
One bottle of 5-hour
Microscope Camera
Computer
15 Daphnia Magna
Procedure
1.
2.
3.
4.
5.
6.
7.
Hook up camera and microscope to computer
Label 5 of the pipettes with the names of the energy
drinks
Label the last pipette Daphnia
Use the daphnia pipette to put a daphnia on the slide
Record the daphnia using the camera for one minute
making sure to be able to see the heart the whole time
Add one drop of monster to the slide using the monster
pipette
Record the daphnia using the camera for one minute
making sure to be able to see the heart beat the whole
time
Procedure (Continued)
8. Add another drop of monster to the slide using
the monster pipette
9. Record the daphnia using the camera for one
minute making sure to be able to see the heart
beat the whole time
10. Continue this for 5 drops then repeat for three
trials
11. Repeat steps 4-9 for each energy drink being
sure to use different slides for each daphnia
and different pipettes for each drink
Monster
Heart Beats per Minute
160
140
120
100
Trial 1
Trial 2
Trial 3
80
60
40
20
0
0
1
2
3
4
Drops of Energy Drink
5
Amp
Heart Beats per Minute
180
160
140
120
Trial 1
Trial 2
Trial 3
100
80
60
40
20
0
0
1
2
3
Drops of Energy Drink
4
5
Nos
Heart Beats per Minute
180
160
140
120
Trial 1
Trial 2
Trial 3
100
80
60
40
20
0
0
1
2
3
Drops of Energy Drink
4
5
Red Bull
Heart Beats per Minute
160
140
120
100
Trial 1
Trial 2
Trial 3
80
60
40
20
0
0
1
2
3
Drops of Energy Drink
4
5
5 Hour
Heart Beats per Minute
180
160
140
120
Trial 1
Trial 2
Trial 3
100
80
60
40
20
0
0
1
2
3
Drops of Energy Drink
4
5
Data Chart
Beats Per Minute
Drink
Trial Base 1drop 2 drops 3 drops 4 drops
Monster 1
138
111
117
129
102
Monster 2
75
51
30
18
9
Monster 3
129
108
117
123
81
Amp
1
141
132
99
75
69
Amp
2
150
135
120
129
117
Amp
3
153
108
69
63
48
5drops
75
0
0
63
87
0
Nos
Nos
Nos
Red Bull
Red Bull
Red Bull
5 Hour
5 Hour
5 Hour
120
123
141
0
84
117
120
0
0
1
2
3
1
2
3
1
2
3
150
93
165
135
141
129
159
129
120
87
144
141
93
129
54
129
123
141
129
129
147
66
78
126
90
99
117
123
150
138
72
96
111
108
111
123
126
147
132
33
87
132
126
0
108
Results
• I saw that the Red Bull had very little affect on
the Crustaceans. I also saw that with most of the
trials the heart rate went up but by the 3rd drop it
started to slow down a lot and stay there. The
Monster and Amp slowed it down but it wasn’t at
a dangerous level. The 5 Hour on the other hand
slowed the hearts down and stopped 2 out of the
3. When they got the Nos they went up and
stayed up through out the trials
Conclusion
• In conclusion it was a tie for the most affect
between 5 hour and Nos. The 5 Hour slowed
their hearts down a whole lot and the Nos sped
them up.
• If I could do my project again I think I might
observe the rates long term to see if the energy
drinks just went through the system or stayed
there. In the future I will separate the ingredients
and see how the different ingredients affect
them.
Summary
• In my experiment I wanted to see the
affects of the energy drinks on Daphnia
Magna. I thought it would speed up the
heart rates until I stopped but I thought
wrong the heart rates sped up but then
slowed down. This is why these drinks are
dangerous. The drink might boost your
energy but, in turn it might slow your heart
down until it stops.
Heart
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