CHP Notebook Table of Contents Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Section 7 Section 8 Section 9 Section 10 Section 11 Section 12 Section 13 Section 14 Safety Program Key Personnel Laboratory Room Assignments Laboratory-Specific Information Emergency Phone Numbers & Procedures Chemical Hazard Assessment (List) Material Safety Data Sheets Laboratory-Specific Standard Operating Procedures Record of Training for Laboratory Personnel Inspections and Exposure Monitoring Accidents, Injuries, and Corrective Actions Disposal of Waste Materials Radiological Hazards Lab and Building Specific Evacuation Information Housekeeping and Maintenance Inspections Section 1 Safety Program Key Personnel In addition to the following personnel, please include your department's safety program key personnel. Name Laura Drabcyk Position EHS Director, Environment, Health & Safety Phone 593-4914 Lou Blatnik EHS Specialist, Environment, Health & Safety 593-0921 Richard Gordon Director, Public Safety 593-1908 Ken Paris Lieutenant, Public Safety 593-5337 __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ Section 2 Laboratory Room Assignments This Chemical Hygiene Notebook pertains to the laboratories and the research facilities of: __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ List all rooms related to the above mentioned lab/research facility. Include all rooms such as temperature controlled, storage closets, and animal facilities. Indicate the faculty who currently direct the indicated rooms. __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ Section 3 Laboratory-Specific Information Each research group should develop its own listings of rooms and personnel. There is no required format; the following can de used as a guide. Name of Principle Investigator: ___________________________________________ Name of Lab Chemical Hygiene Officer: ___________________________________ Lab/Room Number(s): _________________________________________________ Lab Phone Number(s): _________________________________________________ Names and titles of other lab employees and users: ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ Section 4 Emergency Phone Numbers and Procedures Each laboratory group should develop plans and procedures for dealing with emergency situations. As a minimum, this should include phone numbers and evacuation plans. Also refer to the "Emergencies" section of the CHP for additional information. Name of Principle Investigatior (PI): Emergency Phone Number(s): ________________________________ ______________________________ ______________________________ Name of Lab CHO (if different than PI): Emergency Phone Number(s): ________________________________ ______________________________ ______________________________ Names of Other Lab Personnel: Emergency Phone Number: ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ ______________________________ Section 5 Chemical Hazard Assessment (List) Each laboratory is responsible for taking and maintaining its own chemical list. A copy of the current inventory is to be placed in this section. A Chemical List should have a clear title and heading, which includes the following information: • • • • Room number and building name Department Name of person taking inventory Date of inventory There is no specific required format for the information provided in the Chemical List. Lab supervisors should also consider their own needs for chemical management. The inventory should strive to identify the following : • • • • • • Chemical name Location Basic hazard information or classification Approximate quantity If chemical is reordered If quantity changes significantly EHS recommends the following column headings: Quantity Chemical Name Hazard Class Location Date Received Date Removed The Hazard Classifications (possibly more then one per chemical) can be identified with notations such as the following notations (suggested by EHS): A Compressed Gas G Corrosive B Severe Poison H Radioactive C Moderate Poison I Biohazard D Water Reactive J Carcinogenic E Oxidizer K Non-Hazardous F Flammable L "Other” notations It is the responsibility of the Principle Investigator to determine if chemicals in use or in storage produce a potential hazard which must be identified on the Chemical List. Section 5 -- Chemical Hazard Assessment (List) (cont’d) Quantity (include unit of measure) Chemical Name Hazard Class(es) Location Date Received Date Removed Section 6 Material Safety Data Sheets (MSDS) This section should include the location of MSDS for any special materials or particularly hazardous chemicals used in this laboratory. Each lab is responsible for obtaining and maintaining copies of relevant MSDS. Most MSDS can quickly be found through the lings below or a google search. The primary database of MSDS’s for the UM-Dearborn is maintained by Natural Sciences. Each laboratory shall keep a separate MSDS Notebook specific to their area and is to be kept in a specified location (noted below). The MSDS Notebook is located: ____________________________________________. The following links to MSDS Databases are available: EMD Chemicals: http://www.emdchemicals.com/?RedirectedFrom=http://www.emdchemicals.co m/analytics/EMD_Analytics.asp Fisher Scientific: https://www.fishersci.com/wps/portal/CMSTATIC?pagename=msds/ Household Products Database: http://hpd.nlm.nih.gov/ Linde Group: http://msds.lindeus.com/ Mallinckrodt Baker: http://www.mallbaker.com/changecountry.asp?back=/Default.asp Public Health Agency of Canada (Infectious Substances): http://www.phac-aspc.gc.ca/msds-ftss/ Sigma-Aldrich http://www.sigmaaldrich.com/sigmaaldrich/errorpage.html?status=404&r=http%3A%2F%2Fwww.sigmaaldrich.co m%3A4402%2Fsite-level%2Fmsds.html%2F Vermont SIRI Web Page: http://siri.org/msds/index.php VWR Scientific Products: https://www.vwrsp.com/search/index.cgi?tmpl=msds/ Section 7 Laboratory-Specific Standard Operating Procedures (SOP) Each laboratory working with hazardous chemicals should include their own specific Standard Operating Procedures (SOP) here. This section can include laboratory-specific procedures for the following subjects, as they apply for each laboratory, and many other additional topics. A General SOP Template and a Laser SOP Template are available for your use. For additional help, see SOP Examples Chemical procurement, distribution, and storage Working with carcinogens or reproductive toxins Housekeeping, maintenance, and inspections Protective apparel and equipment Dealing with hazardous procedures, substances, and/or equipment Working with radioisotopes Working with biologically hazardous materials Operations requiring special prior approval Any additional special equipment, experimental procedures, or unique hazards A General SOP Template, Particularly Hazardous Materials SOP Template, Laser SOP Template and a Compressed Gas SOP are available for your use. For additional help, refer to the SOP Examples that follow this section. LABORATORY STANDARD OPERATING PROCEDURE (for the use of hazardous materials or equipment) NAME OF PROCEDURE: PREPARED BY: REVISION DATE: LOCATION – This procedure may be performed at the following location(s): HAZARDS – The materials and equipment associated with this procedure present the following exposure or physical health hazards. Safety precautions are prudent and mandatory: ENGINEERING CONTROLS – Prior to performing this procedure, the following safety equipment must be accessible and ready for use, e.g., chemical fume hood, biological safety cabinet, laminar flow hood, chemical spill kits, etc. PROTECTIVE EQUIPMENT – Prior to performing this procedure, the following personal protective equipment must be obtained and ready for use, e.g., acid resistant gloves, safety eyewear, lab coat, chemical splash apron, etc. WASTE DISPOSAL – This procedure will result in the follow regulated waste which must be disposed of in compliance with environmental regulations: ACCIDENTAL SPILL – In the event that a hazardous material spills during this procedure, be prepared to execute the following emergency procedure: Call DPS at 9-1-1 from a campus phone (if after hours) PRIOR APPROVAL – This procedure is considered hazardous enough to warrant prior approval from the Principal Investigator. - YES - NO CERTIFICATION – I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Signature Name (Print) Date Room # < Date this was drafted > < The SOP Title Here > Standard Operating Procedure < Your Name Here > Write a brief description of what this process is used for here. 1.0 Material Requirements: 1.1 Equipment needed for process: List all of the equipment you need to successfully run this process. Be complete. If this is a new process that has not been done in the lab before, consult with lab staff to make sure that equipment you need is available. 1.2 Chemicals needed for process: Include a complete list of the chemicals needed. If specific concentrations, purities, or grades are necessary cite them here. 1.2.1 Hazards associated with chemicals: Include a subsection for each component chemical and if necessary a subsection for the chemical produced. Hazards will be found in the MSDS. Look for information on whether the chemical is flammable, corrosive, toxic, carcinogenic, pyrophoric, an irritant, etc. 1.3 Engineering controls: Where will you run this process? If this process is to be run in a specific location in the lab be explicit. Options include fume hoods, vented ovens, furnaces, glove boxes, wet benches. If this is a new process and the appropriate engineering controls do not seem to be available in the lab, discuss with lab staff whether the process can be done and how to obtain what is needed. If no engineering controls are needed please cite this fact. Some of this information will be in the MSDS. 1.4 Protective equipment needed: What do you wear to protect yourself while performing the process? Check MSDS, but be aware that the protective equipment standards cited in these documents frequently refer to handling the chemical outside of approved engineering controls. If you do not know what protective equipment to use or what types of gloves are compatible with the chemicals you will be using, consult lab staff. 2.0 Procedure: Include detailed instructions on mixing the component chemicals and or use of the gas. Information you should cite will include temperature settings, flow rates/pressure, concentrations, volumes and weights, appearance (if applicable), what the process is supposed to do, how to tell if the process was successful, and what to do with the hardware, chemicals, and equipment after the process is completed. 3.0 Storage: Where will you store this in the lab, e.g., solvent, acid, or base cabinet, refrigerator, etc.? Be aware of incompatibility with other chemicals already in use in the lab. For example, one chemical might react violently with another. In this case you would want to avoid storage in a cabinet with this second chemical. Information will be found in the MSDS. 4.0 Waste Products: How do you dispose of the waste products used in making the material, after using the material or after the material is depleted or gets old. Be specific and describe the specific disposal procedure to be used within the lab, i.e., do not write “Dispose of in accordance with applicable regulations.” Ask members of the laboratory staff for assistance if you do not know how the waste chemical should be handled. 5.0 Incident Procedures: (Found in the MSDS) 5.1 Contact (include a subsection for each component chemical) 5.1.1 Skin: 5.1.2 Eyes: 5.1.3 Inhalation: 5.1.4 Ingestion: 5.2 Spill or leak: How do you deal with a small spill? Are there specific absorbents that should be used? Is the disposal procedure you cited in section 4 valid in case of a spill? If it’s a gas leak, what do you do when the detectors go off? Be specific. Do not include the chemical manufacturer’s emergency numbers here. 5.3 Fire: Are there specific concerns to be considered in the event of a fire? For example, some chemicals are water reactive, and using water on a fire where these chemicals are involved will make the problem worse. For emergencies during non-business hours, call the Dept. of Public Safety at 91-1 (from any campus phone) or 313-593-5333. LABORATORY STANDARD OPERATING PROCEDURE (SOP) FOR THE USE OF PARTICULARLY HAZARDOUS SUBSTANCES PAGE 1 This template SOP is designed to provide guidance in writing procedures for the safe handling and disposal of particularly hazardous substances including carcinogens, engineered nanomaterials, hazardous drugs and toxins. This template must be customized and reviewed for its applicability to the specific procedure listed below. See Section 8.5 of the Chemical Hygiene Plan (Materials and Procedures Requiring Special Provisions) for detailed information under what conditions a specific SOP is required. If you have questions concerning the applicability of any item listed in this procedure contact the Principal Investigator/Laboratory Supervisor or Environment, Health & Safety 313-593-4914. TABLE OF CONTENTS: Section Page Procedure Title, Description, Preparer, Revision Date Location, Designated Area Hazards Control of Hazards Protective Equipment Decontamination Procedures Special Handling and Storage Waste Disposal Accidental Spill Training Requirements Prior Approval, Medical Surveillance determination Certification of Training Attachments Appendix A Animal Handling Procedures Supplement 1 1 2 2 3 4 4 4 4 5 5 6 7 SPECIFIC PROCEDURE TITLE: PREPARED BY: REVISION DATE: DESCRIPTION OF PROCEDURE: LOCATION: These procedures must be performed in a designated area. Describe location(s): ________________________________________________________________________ A designated area shall be established where limited access, special procedures, knowledge, and work skills are required. A designated area can be the entire laboratory, a specific laboratory workbench, or a laboratory hood. Designated areas must be clearly marked with PARTICULARLY HAZARDOUS SUBSTANCES SOP PAGE 2 signs that identify the hazard and include an appropriate warning; for example: WARNING! FORMALDEHYDE WORK AREA – CARCINOGEN. Upon leaving the designated area, remove any personal protective equipment worn and wash hands, forearms, face, and neck. After each use, wipe down the immediate work area and equipment to prevent accumulation of chemical residue. At the end of each project, thoroughly decontaminate the designated area before resuming normal laboratory work in the area. Storage or consumption of food, storage or use of containers of beverages, storage or application of cosmetics, smoking, storage of smoking materials, tobacco products or other products for chewing, or the chewing of such products are prohibited in designated areas. Designated areas shall be maintained under negative pressure with respect to corridors and public areas. HAZARDS: The chemicals associated with this procedure present exposure or physical health hazards. Safety precautions are prudent and mandatory. The MSDSs for the chemicals used in the area must be accessible to lab personnel. MSDS location: ______________________________________________________________. Select the hazard class: (circle all that apply) Carcinogen, chemotherapeutic agent, engineered nanomaterial, mutagen, embryotoxin, highly toxic, sensitizer. Other: (describe) ___________________________________. Route of exposure: (circle all that apply) Inhalation, contact, ingestion, injection. Major target organs: (circle all that apply) Skin, liver, lung, kidney, reproductive, CNS. Physical chemical properties: (circle all that apply) Flammable, explosive, reactive, corrosive, peroxide forming. Add specific chemical hazard information here: Example: Long-term risk of occupational exposure to cytotoxic (Chemotherapeutic) hazardous drugs (HD) has been associated with human cancers at high (therapeutic) levels of exposure. These drugs have been shown to be carcinogens, mutagens and teratogens in many animal species. There is evidence that hazardous drugs may cause spontaneous abortions and increase the risk of congenital malformations. In addition, some of these drugs have been shown to cause acute effects in humans, such as localized skin necrosis (death of tissue) damage to normal skin after surface contact, dizziness, lightheadedness. Adverse health effects from both acute and chronic exposures have been demonstrated in health care personnel. All of these drugs have potential to damage cells or adversely affect cellular growth and reproduction. The drugs bind directly to genetic material in the cell nucleus, or affect cellular protein synthesis. Employees can be exposed to hazardous drugs PARTICULARLY HAZARDOUS SUBSTANCES SOP PAGE 3 through inhalation of drug dust or droplets, absorption through the skin directly, injection through the skin or ingestion through contaminated food. CONTROL OF HAZARDS: Methods used to control hazards will rely on engineering controls over administrative controls and personal protective equipment. Type of engineering control utilized: (circle) Fume hood, negative pressure glove box, sealed system, Class II Biological Safety Cabinet (BSC), other local exhaust system. Indicate specific hazard controls that must be utilized to perform this procedure. __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ _______________________________________________ Additional required procedures: Although the specific SOPs will vary according to the material used, the following guidelines are generally applicable for projects involving particularly hazardous substances: Use the smallest amount of chemical that is consistent with the requirements of the work to be performed. Use containment devices (such as lab fume hoods or glove boxes) when: (i) volatilizing these substances, (ii) manipulating substances that may generate aerosols, and (iii) performing laboratory procedures that may result in uncontrolled release of the substance. Use high efficiency particulate air (HEPA) filters, carbon filters, or scrubber systems with containment devices to protect effluent and vacuum lines, pumps, and the environment whenever feasible. Use ventilated containment to weigh out solid chemicals. Alternatively, the tare method can be used to prevent inhalation of the chemical. While working in a laboratory hood, the chemical is added to a pre-weighed container. The container is then sealed and can be re-weighed outside of the hood. If chemical needs to be added or removed, this manipulation is carried out in the hood. In this manner, all open chemical handling is conducted in the laboratory hood. Use containment devices for the following procedures that may also present opportunities for employee exposure during preparation: Withdrawal of needles from drug vials; Drug transfers using syringes and needles; Breaking open ampoules; Expulsion of air from drug-filled syringe. If the process does not permit the handling of such materials in an OSEH certified ventilation control device, contact EHS at 313-593-4914 to review the adequacy of control measures. PARTICULARLY HAZARDOUS SUBSTANCES SOP PAGE 4 PROTECTIVE EQUIPMENT: Prior to performing this procedure, the following personal protective equipment must be obtained and ready for use: (ex. acid resistant gloves, safety eyewear, lab coat, chemical splash apron): __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ Hand Protection - Select appropriate glove type and double glove. For proper selection of glove material, review chemical MSDS and glove manufacturer’s glove selection guides (see OSEH web site for links). Glove Type: (circle appropriate glove material) nitrile, neoprene, vinyl, latex, laminate. Gloves should be changed frequently and should be pulled up over outer sleeves to reduce skin exposure. Where to Find Compatibility Information Most glove manufacturers have chemical compatibility charts available for their gloves. These charts may be found in laboratory supply catalogs such as Fisher Scientific and Lab Safety Supply. Best Gloves. Best Gloves also offers an excellent on-line database for glove selection at www.bestglove.com. Eye Protection – Select appropriate eye protection. Eyewear: (circle appropriate type) safety glasses, Safety goggles Goggles (not safety glasses) are appropriate for processes where splash, spray, or aeresolization is foreseeable. Other Protective Clothing At minimum, lab coat, long pants, and closed toed shoes are to be worn when entering laboratories having hazardous chemicals. Hazardous chemicals that are toxic via skin contact/absorption may require additional protective clothing (i.e., face shield, apron, oversleeves, bonnets) as appropriate where chemical contact with the body/skin is foreseeable. Respiratory protection – Is not required when proper engineering controls are implemented. In some cases a N95 disposable respirator can be used as an added layer of protection from particulates or a potential splash. Respirator usage may require training, fit testing and a medical evaluation. Contact OSEH (7-1143) to determine requirements for specific applications. PARTICULARLY HAZARDOUS SUBSTANCES SOP PAGE 5 DECONTAMINATION PROCEDURES Personnel decontamination: Immediately after working with particularly hazardous materials, remove gloves, wash hands and arms with soap and water. Any time you leave the designated area you must remove protective clothing and conduct personnel decontamination. Area decontamination: Decontamination procedures vary depending on the material being handled; consult the MSDS. Consideration should be given to neutralizing some agents with other reagents as part of the decontamination process; consult the MSDS, EHS, and other resources to determine applicable methods. All surfaces should be wiped with the appropriate cleaning agent following dispensing or handling. Waste materials generated should be treated as a hazardous waste. In the absence of other methods, decontamination should consist of surface cleaning with water and detergent followed by thorough rinsing. The use of detergent is recommended because there is no single accepted method of chemical deactivation for all agents involved. A plastic backed absorbent pad should be placed under the work area during the process. This should be changed at the end of each process or when a spill occurs. Equipment decontamination: Decontaminate glassware, vacuum pumps or other contaminated equipment before removing from the designated area. Attach the EHS Equipment Decontamination form to equipment to be sent offsite. Biological Safety Cabinets used for antineoplastic preparation should be cleaned daily with 70% ethanol solution and decontaminated weekly and whenever spills occur. Decontamination procedures should include surface cleaning with high pH agents; thorough rinsing, removal, and cleansing of work trays; and sump cleansing. SPECIAL HANDLING AND STORAGE Note storage location:__________________________________________________________________ Label containers and storage areas with strong warnings like "CANCER-SUSPECT AGENT". Ensure secondary containment and segregation of incompatible chemicals per guidance within the Chemical Hygiene Plan. Follow any substance-specific storage guidance provided in MSDS documentation. PARTICULARLY HAZARDOUS SUBSTANCES SOP PAGE 6 WASTE DISPOSAL - This procedure will result in the following regulated waste which must be disposed of in compliance with environmental regulations: ____________________________________________________ All particularly hazardous substance waste including gloves, syringes, vials, and solution containers should be placed in a labeled 5 gallon white pail. Needle syringe assemblies must be disposed in sharps containers with hazardous waste labels. Needles must not be recapped for disposal. The waste container must and be located within the designated area and should be closed except when actively adding waste. Contact EHS at 313-593-4914 for supplies and to schedule removal of waste. ACCIDENTAL/INCIDENTAL SPILL: Prompt response to chemical spills is critical to protect worker health and safety and to mitigate adverse affects to the environment. Spills should be identified with a warning sign to limit access to the area until decontamination has been completed. Spills should be cleaned up immediately by a properly protected employee who has been trained in the appropriate procedures regarding the handling and disposal of hazardous substances. Spills should be cleaned with a mild detergent and rinsed twice with water. All contaminated cleanup materials should be disposed of in the appropriately labeled waste container. Call EHS at 313-593-4914 for disposal of contaminated waste materials and for assistance in cleaning the spill. In case of a spill onto employee's skin or eyes, quick response to the nearest emergency shower and eyewash location is necessary to reduce exposure. Remove contaminated clothing and gloves. Don clean gloves and wash the affected skin area with soap and water. For eye contact, flush the eye with water for 15 minutes and seek medical attention. Employees should be referred to Midwest Health Service, the UM –Dearborn Occupational Health Provider, for treatment. Any exposure must be reported on the WorkConnections Injury and Illness form and sent to WorkConnections. Spills of powdered material should be cleaned up by personnel wearing appropriate respiratory protection, double protective gloves and gowns. Wet towels should be placed over the spilled material, and water should be used to absorb any dry powder. Materials used in clean-up should be placed in appropriate waste barrels and treated as hazardous waste. Laboratory personnel who work with hazardous chemicals are to be provided the opportunity to receive medical attention/consultation when: A spill, leak, explosion or other occurrence results in a hazardous exposure (potential overexposure). Symptoms or signs of exposure to a hazardous chemical develop. Specific emergency procedures shall be posted and employees shall be familiar with them. PARTICULARLY HAZARDOUS SUBSTANCES SOP PAGE 7 TRAINING REQUIREMENTS EHS general laboratory safety training as well as laboratory-specific training is required. Each employee, prior to being authorized to enter a designated area, shall receive training from the PI or an experienced lab manager that includes but not necessarily limited to: The nature of the hazard, including local and systemic toxicity; The specific nature of the operation that could result in exposure; The purpose and application of the medical surveillance program, including, as appropriate, methods of self-examination; The purpose and application of decontamination practices and purposes; The purpose and significance of emergency practices and procedures; The employee's specific role in emergency procedures; Specific information to aid the employee in recognition and evaluation of conditions and situations which may result in the release of chemicals addressed by this section; The purpose and application of specific first aid procedures and practices; PRIOR APPROVAL: This procedure is considered hazardous enough to warrant prior approval from the laboratory director. -YES-NOMEDICAL SURVEILLANCE: This procedure requires medical surveillance through the EHS Medical Surveillance program (contact EHS at 313-593-4914 for assistance on a determination of medical surveillance). -YES-NOCERTIFICATION: I have read and understand the above SOP. I agree to contact my supervisor or lab director if I plan to modify this procedure. Signature Name (Print) Date Room # __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ COMPRESSED GAS CYLINDER SOP TEMPLATE < Date this was drafted > Compressed Gas Cylinder Handling and Use Standard Operating Procedure < Your name here > 1.0 Material Requirements: 1.1 Equipment needed: List all of the equipment you need. Be complete. Include cylinder cart, bubble soap, helium leak check device, etc. 1.2 Gases Used in < XXXXXX > Lab: Include a complete list of the gases used by your research group. Specify concentrations, purities, or mixtures. 1.2.1 Hazards associated with gases: Include a subsection for each gas. Hazards will be found in the MSDS. Look for information on whether the gas is flammable, corrosive, toxic, carcinogenic, pyrophoric, an irritant, etc. 1.3 Engineering controls: Options include gas cabinets, local exhaust ventilation, cylinder storage racks, bench mounted restraints, etc. Compressed gas cylinders must be secured in an upright position away from excessive heat, highly combustible materials, and areas where they might be damaged or knocked over. Be specific as to location where the gases will be used, especially if this SOP applies to more than one laboratory in your research group. This section must also include information on what tubing material is or is not allowed and information on regulator selection and use. Never interchange regulators and hose lines (with one type of gas for another). Explosions can occur if flammable gases or organic materials come in contact with oxidizers (e.g., oxygen) under pressure. 1.4 Protective equipment needed: What do you wear to protect yourself while changing the cylinders or using the gases? Check MSDS, but be aware that the protective equipment standards cited in these documents frequently refer to handling the gas outside of approved engineering controls. If you do not know what protective equipment to use, consult EHS. 2.0 Procedure: Include detailed instructions on transporting the cylinders, securing the cylinders, changing the cylinders, attaching tubing and regulators, leak-check procedures and use of the gas. Information you should cite will include appropriate flow rates/pressure, concentrations, volumes and weights (if applicable), how to tell if the cylinder is leaking, and what to do after the process is completed. NOTE: If you have a gas cabinet and/or gas detection system you will probably want to have a separate SOP for operation and maintenance of those systems. 3.0 Storage: Where will you store the cylinders in the lab? Cylinders must be stored in dry, wellventilated areas. Closets and lockers would not be acceptable storage locations. Cylinders must be stored with the protective caps in place. How much gas do you intend to keep on hand? Under no circumstances should a researcher purchase more than a 4-year supply (the normal course of a research project). Be aware of incompatibility with other gases already in use in the lab. For example, cylinders of oxygen and other oxidizers must be stored at least 20-feet from fuel-gas or other combustible materials unless separated by a noncombustible wall, not less than 5-feet high, having a fire-resistance rating of ½-hour. Information will be found in the MSDS. 4.0 Empty Cylinders: At what point are cylinders considered “empty”, and where do they go when they are empty. Be specific and describe the cylinder return procedure to be used within the lab, i.e., do not write “Dispose of in accordance with applicable regulations” or “Return to storage area for pickup by supplier.” 5.0 Accident Procedures: (Found in the MSDS) 5.1 5.2 5.3 Contact (include a subsection for each gas) 5.1.1 Skin: 5.1.2 Eyes: 5.1.3 Inhalation: 5.1.4 Ingestion: Gas leak: How do you deal with a small leak? A larger leak? Be specific with regard to UM procedures and phone numbers. Do not simply include the gas supplier’s emergency numbers here. If your lab has a gas alarm, indicate what procedures must be followed when the alarm sounds. Fire: Are there specific concerns to be considered in the event of a fire? For example, some chemicals are water reactive, and using water on a fire where these chemicals are involved will make the problem worse. Include the following information: Report all accidents, e.g., injuries, leaks, fires, etc., to the . . . (fill in, as applicable for your research group). This could be the Principal Investigator, Laboratory Director, etc. You will also need to submit an incident report to the Public Safety For emergencies during non-business hours, call Public Safety at 9-1-1 (from any campus phone) or (313-593-5333. Section 8 Record of Training for Laboratory Personnel Records of training sessions attended by each person using the laboratory (including the supervisor) should be kept in this section. Records should include the laboratory user’s name, the type of training (e.g., Lab Safety Lecture, Lab-Specific Training, etc.), who conducted the training (lab supervisor, etc.), and the date of the session. Records of training or training certificates provided by EHS should also be added to this section. Two different forms are provided to document lab specific training. Both forms are useful and may be used as needed. A training checklist is also included to cover labsafety basics as well as specific training. This checklist can be used as a guide to topics that should be covered or as an actual training document that is completed for each employee. If used as a training record it should be signed and dated. CHEMICAL HYGIENE TRAINING FORM Name & UMD ID # Type of Training Conducted By University of Michigan-Dearborn Date Certification of Personal Protective Equipment Training I, , certify that the following affected employees (print full name) have received and understood personal protective equipment (PPE) training, which included the following: when PPE is necessary; what PPE is necessary; how to properly don, doff, adjust, and wear PPE; the limitations of the PPE; and the proper care, maintenance, useful life and disposal of the PPE. Each of the affected employees has demonstrated an understanding of the above and an ability to use the PPE properly. This training is in compliance with 29 CFR 1910.132 (f) and MIOSHA Part 33, Rule 408.13300. Name UM ID Number Date of Training Equipment Type (signature) (date) Note to signer: maintain this certification with your permanent records. EMPLOYEE SPECIFIC CHEMICAL HYGIENE TRAINING FORM Name ___________________________________________ Department ______________________________________ Campus Location __________________________________ Campus Phone Number______________________________ Employee Title _____________________________________ Supervisor _________________________________________ OSHA's Laboratory Standard (29CFR1910.1450) and MIOSHA Part 431, Rule 325.70100, requires that each laboratory employee be made aware of the location and content of the laboratory's Chemical Hygiene Plan. By your signature below, you acknowledge that you have read and understood the contents of this plan and know its location within the laboratory. ____________________________________________ ____________________ Employee Signature Date The Laboratory Safety Standard further requires that the employee's supervisor provide training, which covers the specific topics described in the "Information and Training" section of the Chemical Hygiene Plan. This training must be provided at the time of the employee's initial assignment, on a refresher basis at least annually and upon updating procedures. Document specific training below: Description of Training Date Training Checklist Provided By This checklist may be used to assist with the laboratory specific training requirements outlined in the Laboratory Safety Standard. Introduction to laboratory specific Chemical Hygiene Plan (CHP): Location and contents Introduction to laboratory specific Standard Operating Procedures (SOP’s) Review Chemical Inventory Review Location of MSDS’s Review emergency information: Spills, Personal Injury, Fire, Power Failure Fire extinguisher First Aid Supplies Evacuation Plans Basic Safety Rules Note rules with special importance for your laboratory. Identify specific areas for food consumption. Review procedures for working after hours. Review the Chemical Inventory for your laboratory Review the UM-Dearborn Waste Handling Procedures Labeling Packaging Pick-ups Review procedures for chemical procurement, distribution, and storage Review Standard Operation Procedures for use of toxic materials Storage (acid cabinet, flammable liquid storage cabinet, flammable liquid storage refrigerator, etc.) Personal Protective Equipment (PPE) Location of procedure (mechanical ventilation required) Waste Disposal (aqueous, solid, biohazardous, radioactive) Review Procedures for Use of Compressed Gas Cylinders Protective Apparel and Equipment Discuss when safety glasses, goggles, or face shields are required. Discuss any need for other protective equipment Discuss selection of gloves. Housekeeping, Maintenance, and Inspections Discuss materials stored or frequently present on the floor. Discuss maintenance items for scientific equipment. Discuss formal and internal inspection programs. Environmental Monitoring Discuss PEL's and TLV's for chemicals in use and how to reduce employee exposure. Discuss building ventilation. Discuss use of fume hoods, biological safety cabinets or other mechanical ventilation systems. Review SOP for working with biologically hazardous materials Review Exposure Control Plan if working with human blood or other potentially infectious materials. Discuss Biosafety Manual if working with recombinant DNA or infectious agents. Review Hepatitis B Vaccination Program. Review PPE, Housekeeping and Waste Disposal Procedures. Working with Radioisotopes Review radiological safety practices Review Dosimetry Program Medical Program Discuss need for any medical surveillance (Respirator use, Potentially Hazardous Materials, Heat Stress, Noise, Animal Handler, Laser Operator, etc…) contact EHS (313-593-4914) for assistance. Training Program Discuss Departmental Specific and EHS training sessions. Additional Safety Session Topics Review recent incidents/accidents/injuries and how to prevent reoccurrence. Review new equipment at least annually. Review new procedures at least annually. Review results of recent inspections and how to correct problem areas. Section 9 Inspection and Exposure Monitoring Records This section should contain information on laboratory inspections and exposure monitoring (including the date and recommendations). Inspections may be conducted by individual research groups, EHS, MIOSHA, NRC, and possibly other organizations. This section should also contain records associated with corrective actions. Please refer to one of the following for your laboratory: Laboratory Safety Checklist, Machine Shop Safety Inspection, or the Laser Safety Inventory & Inspection Sheet. __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ Section 10 Housekeeping and Maintenance Inspections Periodic housekeeping and maintenance self-inspections are recommended. The inspections should include equipment, structural integrity, and personal protective equipment. This section should note who is responsible for this inspection, how often inspections will be conducted and where the records are kept. The table below can be utilized for maintenance inspections. If inspections are conducted using OSEH’s Laboratory Safety Checklist, this will satisfy the housekeeping requirement because housekeeping is one of the items on this checklist. Inspector: Date of Inspection: Location of Records: Equipment Satisfactory Semi-annual Laboratory Safety Checklist Unsatisfactory Building: Comments / Action Rooms: Lab Manager: Inspector: Date: Yes / No / NA Written Materials: Door signs posted with contact personnel: Emergency numbers posted by telephone: Chemical hygiene plan with SOPs present: Personnel aware of CHP & ECP Training: Chemical inventory for all chemicals: Personal protective equipment present and used: Laboratory coats: Gloves (Disposable & Chemical): Safety glasses/goggles/face shield: Other (apron, respirator, ear plugs, etc): Safety equipment present and in working condition: Emergency shower & inspection date current: Emergency eye-wash & inspection date current: Drench hose: Fire extinguisher & inspection date current: Fumehood & inspection date current: Biological safety cabinet & inspection date current: Spill kits readily available: Chemical Storage: All containers of chemicals are labeled: Gas cylinders are secured and capped: Chemicals are stored on shelves/in cabinets: Chemicals are stored by class: Large containers are on low shelves: Electrical Hazards: Extension cords are prohibited: Outlet extenders are prohibited: Electrical cords are in good condition: Surge suppressors/multi-outlets used properly: General Safety: Aisles are kept clear: Work area is neat: Eating/drinking/smoking prohibited in lab: Waste Handling: Chemical waste managed properly: Biological waste managed properly: Radiological waste managed properly: Glassware/plastics/sharps managed properly: Section 11 Accidents, Injuries, and Corrective Actions Comments Include in this section a description of laboratory accidents and corrective actions taken to prevent them in the future. It is always important to take a careful look for all underlying and precipitating causes. Copies of any EHS, MIOSHA, or other accident-related forms should be maintained here. Please note: An Injury or Illness Report Form must be completed for all employee workplace injuries and illnesses go to http://www.umich.edu/~connect/forms.htm click on “Illness and Injury Report form”. An injury or illness report form for non-employee injuries and illnesses must be completed, go to http://www.umich.edu/~riskmgmt/forms.html click on “Injury Report for Non-Employees”. This section should contain records pertaining to chemical spills and medical assistance. __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ Section 13 Disposal of Waste Materials Hazardous waste disposal must be coordinated with the EHS at 313-593-4914 assistance and scheduling of a waste pick up. Type of waste generated from area: __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ Person(s) responsible for waste labeling in this lab (if you need assistance EHS at 313-593--4914): __________________________________________________________________________ __________________________________________________________________________ List here any lab specific procedures that are not covered in your SOPs. __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ _________________________________________________________________________ __________________________________________________________________________ For waste pick up call EHS at 313-593-4914. Section 14 Radiological Hazards Various types of records are required for regulatory compliance in all laboratories using radioactive materials. Copies of these forms and other documents should be maintained in this section. If maintained in the Radiation Safety Manual, include location of this manual. If applicable please complete __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ Section 14 Lab and Building Specific Evacuation Information * For emergency information dial 911 Laboratory Supervisor/Emergency Coordinator for this area: _____________________________________ Designated assembly areas: ______________________________________ ______________________________________ Emergency Egress Map Example Public Safety & Environmental Health maintains building egress maps. It is recommended that each laboratory supervisor maintains an egress layout for their lab. Section 13 Housekeeping and Maintenance Inspections Periodic housekeeping and maintenance inspections are required. The inspections should include equipment, structural integrity, and personal protective equipment. This section should note who is responsible for this inspection, how often inspections will be conducted and where the records are kept. The table below can be utilized for maintenance inspections. Inspector: ___________________________________________ Date of Inspection: ___________________________________________ Location of Records: ___________________________________________ Equipment Satisfactory Unsatisfactory Comments/Action University of Michigan - Dearborn Environment, Health & Safety (EHS) LABORATORY EQUIPMENT DECONTAMINATION FORM EQUIPMENT OWNER Principal Investigator: ____________________________________________ Department: ____________________________________________________ Contact Phone Number: ___________________________________________ Building: _______________________________ Room Number: _________ EQUIPMENT INFORMATION Equipment Type/Model: __________________________________________ Serial Number: __________________________________________________ Service/Transportation/Disposal Provider: ____________________________ Service to be performed: __________________________________________ RADIOACTIVE MATERIALS: If radioactive materials were used or stored in the equipment, contact OSEH Radiation Safety Services (RSS) at 734-764-6200 to conduct a survey. Did the RSS survey indicate detectable levels of radioactive contamination? Check one Yes No N/A CERTIFICATION OF DECONTAMINATION: I certify that the above referencedlaboratory equipment has been thoroughly cleaned and decontaminated of all chemical,biological, and radioactive contaminants. _____________________ (Name Print) ________________________ ____________ (Signature) (Date) Complete and attach this form to equipment used for hazardous materials that is sent outside the laboratory. Contact EHS at 313-593-4914 for questions about decontamination or completing the form. SAMPLE A LABORATORY STANDARD OPERATING PROCEDURE (for the use of hazardous materials or equipment) Name of Procedure: Use of Benzene for Gel Electrophoresis Prepared By: <Preparer’s Name> Revision Date: 05/01 LOCATION – This procedure may be performed at the following location(s): All procedures with benzene will be conducted in a chemical fume hood located in room 1234 ABC. Keep in tightly closed container. Store in rated flammable liquid storage cabinet. Store with compatible materials, e.g., away from oxidizers. USAGE – The following is a brief summary of how this material will be used. One (1) liter container of benzene will be ordered. Each use will need 400 ml; two runs/per experiment. Only one person will perform and use this gel system. There will be no more than 3 runs (6 gels) performed between now and July 15, 2001. The employee has previous training and experience with this protocol in Brazil. No other employees will be performing this procedure after July 2001. See Protocol for additional details. HAZARDS – The materials and equipment associated with this procedure present the following exposure or physical health hazards. Safety precautions are prudent and mandatory: Carcinogen: Chronic exposure by inhalation may result in various blood disorders (anemia, leukemia). Symptoms of acute exposure can lead to headaches, dizziness, nausea or intoxications. May irritate eyes, nose and respiratory tract. Benzene can be absorbed through the skin and may cause dermatitis. Flammable ENGINEERING CONTROLS – Prior to performing this procedure, the following safety equipment must be accessible and ready for use, e.g., chemical fume hood, biological safety cabinet, laminar flow hood, chemical spill kits. This procedure must be conducted in a certified chemical fume hood located in room 1234 ABC. (Fume Hood Certification date 01/25/01) PROTECTIVE EQUIPMENT – Prior to performing this procedure, the following personal protective equipment must be obtained and ready for use: Employee should double glove for prior to working with benzene. Disposable nitrile gloves provide minimum protection for general laboratory use and should be changed frequently or whenever contamination is suspected. Viton® gloves are required when hand immersion in benzene is expected. Safety goggles should be worn when a splash hazard exists; safety glasses with side shields are required at a minimum when benzene is used in a closed system. A laboratory coat should be worn when working with chemicals. A chemically resistant apron should be used if transferring or using large quantities of benzene in open containers WASTE DISPOSAL – This procedure will result in the following regulated waste that must be disposed of in compliance with environmental regulations: Benzene waste will be collected by manual aspiration of material. It will be placed in a glass bottle, which will have a hazardous waste label and radioactive label. This waste must be discarded within 90-days. The following information must be filled out on the label before hazardous waste can be placed into the bottle: identification of contents (Benzene), accumulation start date (the date material is first placed into the bottle as waste), as well as the name of a laboratory researcher (Jim Smith), room number and building (123 ABC). EHS (313-593-4314) will be contacted to dispose of the material. ACCIDENTAL SPILL – In the event that a hazardous material spills during this procedure, be prepared to execute the following emergency procedure: If a small spill: Absorb with compatible material such as vermiculite or spill pads and transfer absorbed material to a closed container. Label and date as hazardous waste. Contact EHS for pickup (313-593-4914) If a large spill: Notify others in room of spill. Extinguish all ignition sources; if safe to do so. Evacuate room/immediate area. Call EHS (313-593-4914) or DPS (9-1-1 from at campus phone or 313-593-5333) for cleanup. Post room with warning notifying others of spill. Prevent unnecessary entry into area until arrival of EHS. Provide assistance and information to spill responders. In the event of a splash to the eyes or skin, immediately flush with water for 15minutes and seek medical attention at Midwest Health System. PRIOR APPROVAL – This procedure is considered hazardous enough to warrant prior approval from the Principal Investigator. YES NO Principal Investigator’s Signature:____________________________________________________________ ADDITIONAL PRECAUTIONS & REFERENCES: Keep away from ignition sources. Incompatible with strong oxidizers or strong acids. Wash hands thoroughly after use. Do not eat, drink or smoke in areas where benzene or other chemicals are used. LABORATORY STANDARD OPERATING PROCEDURE (for the use of hazardous materials or equipment) Name of Procedure: Use of Methylene Chloride Prepared By: < Preparer’s Name > Revision Date: 05/02/04 LOCATION – This procedure may be performed at the following location(s): All procedures with methylene chloride (MeCl2) will be conducted in a chemical fume hood located in lab at 321. Keep in tightly closed container. Store in rated flammable liquid storage cabinet. Store with compatible materials, e.g., away from oxidizers, plastics, rubber, nitric acid and chemically active metals. USAGE – The following is a brief summary of how this material will be used. Methylene Chloride is used as an extraction solvent. It removes Semi-Volatile Organic Compounds (SVOC), Pesticides, and PCBs from both water and soil samples. Typically 50 ml are used per sample. The only difference is SVOC waters. To do those, add 60 ml MeCl2, shake, drain the MeCl2, and repeat five more times. Total MeCl2 300 ml per sample. All methylene chloride work is done inside a fume hood. The soil extraction unit is outside the hood, but it is a self-contained system. See specific protocols for additional details. HAZARDS – The materials and equipment associated with this procedure present the following exposure or physical health hazards. Safety precautions are prudent and mandatory: Suspect Carcinogen (of bile duct and brain). Chronic exposure can cause headache, mental confusion, depression, liver and kidney effects, and bronchitis. Symptoms of acute exposure can lead to mental confusion, headaches, dizziness, nausea or intoxications. May irritate eyes, nose and respiratory tract. Methylene Chloride can be absorbed through the skin and may cause dermatitis or chemical burns. Flammable. ENGINEERING CONTROLS – Prior to performing this procedure, the following safety equipment must be accessible and ready for use, e.g., chemical fume hood, biological safety cabinet, laminar flow hood, chemical spill kits. This procedure must be conducted in a certified chemical fume hood located in lab. PROTECTIVE EQUIPMENT – Prior to performing this procedure, the following personal protective equipment must be obtained and ready for use: Employee should double glove prior to working with methylene chloride. Disposable neoprene gloves provide minimum protection for general laboratory use and should be changed frequently or whenever contamination is suspected. Safety goggles should be worn when a splash hazard exists; safety glasses with side shields are required at a minimum when methylene chloride is used in a closed system. A laboratory coat should be worn when working with chemicals. A chemically resistant apron should be used if transferring or using large quantities of methylene chloride in open containers WASTE DISPOSAL – This procedure will result in the follow regulated waste which must be disposed of in compliance with environmental regulations: Methylene chloride waste will be collected by manual aspiration of material. It will be placed in a glass bottle, which will have a hazardous waste label. This waste must be discarded within 90-days. The following information must be filled out on the label before hazardous waste can be placed into the bottle: identification of contents (Methylene Chloride), accumulation start date (Date material is collected in bottle as waste), as well as the name of a laboratory researcher, room number and building. Contact EHS (313-593-4914) to dispose of the material. ACCIDENTAL SPILL – In the event that a hazardous material spills during this procedure, be prepared to execute the following emergency procedure: If a small spill: Absorb with compatible material such as vermiculite or spill pads and transfer absorbed material to a closed container. Label and date as hazardous waste. Contact EHS for pickup (313-593-4914). If a large spill: Notify others in room of spill. Extinguish all ignition sources. Evacuate room/immediate area. Call EHS (313-593-4914) or DPS (9-1-1 from a campus phone or 313-593-5333) for cleanup. Post room with warning notifying others of spill. Prevent unnecessary entry into area until arrival of EHS. Provide assistance and information to spill responders. In the event of a splash to the eyes or skin, immediately flush with water for 15minutes and seek medical attention at Midwest Health Services. PRIOR APPROVAL – This procedure is considered hazardous enough to warrant prior approval from the Principal Investigator. YES NO Principal Investigator’s Signature:____________________________________________________________ ADDITIONAL PRECAUTIONS & REFERENCES: * Keep away from ignition sources. Incompatible with strong oxidizers and metals. * Wash hands thoroughly after use. Do not eat, drink or smoke in areas where methylene chloride or other chemicals are used. Chemical Hygiene Plan: Standard Operating Procedure Examples (Must be customized for each particular laboratory.) Written safety, handling and waste disposal procedures are required for all hazardous chemicals used in the laboratory. This includes all chemicals that are toxic, carcinogens, reproductive toxins, irritants, corrosives, sensitizers, hepatotoxins, nephrotoxins, neurotoxins, agents that act on the hematopoietic systems, and agents that damage the lungs, skin, eyes, or mucous membranes. These procedures must be designed to adequately protect the safety and health of workers in the laboratory and specify specific control measures including: Engineering Controls, e.g., Fume Hood, Personal Protective Equipment, e.g., acid-resistant gloves, and Administrative Controls, e.g., limit duration of exposure or reduce quantities involved in the process. Most chemicals that are found in the laboratory can be grouped into categories, e.g., acids, flammable liquids, etc., that have the same safety, handling and waste disposal procedures. Chemicals fitting into these categories can be covered by general written procedures designed for that category. Chemicals that present a particular hazard that is not adequately covered in the general health and safety procedures must have specific Standard Operating Procedures (SOP) that protect the worker. These procedures must consider how the chemical is being used and all dangers involved with its use. Beyond writing the SOP, the workers must also be trained in the use of these procedures. This includes the proper use of safety equipment and engineering controls. Following are examples of both general and specific SOP. General SOP: Acids (strong acids, may cause skin and eye burns) Skin and eye contact should be avoided at all times with the use of personal protective equipment. All persons in the lab should wear safety glasses. Face shields, goggles and shields should be used to provide better protection when pouring or manipulating large volumes. Protection from contact with the skin is achieved through the use of gloves, laboratory coats, tongs, and other protective devices. Gloves should be chosen with a high resistance to acids, like Nitrile NBR gloves. The Nitrile NBR gloves and safety goggles are stored in drawer XYZ. Always add acids to water when mixing. Spills must be cleaned up immediately with the acid spill kit located in cabinet XYZ in room ABC. Most acids are very slippery and may create a slip and fall hazard when spilled on the floor. In the event of skin or eye contact, the affected area should be rinsed with water for at least 15-minutes. Medical attention should be sought as soon as possible. Notify the lab supervisor and report to Midwest Health Services. Acids must be stored in the corrosive-resistant cabinet, with other compatible chemicals, marked “acid” under the fume hood. Acids should never be stored with flammables or organics. Do not store above eye level or out of view. Manifest, label and dispose of acid waste according to EHS guideline. Call EHS at 313593-4914 for pick up of hazardous waste. Specific SOP: The SOP can be written for individual chemicals as shown below or as a preamble to your experimental protocols. A “safety precautions” section to your current protocols should include the same information including engineering controls, PPE and the hazards involved. Use whichever method is easier for you; both are in compliance with the Laboratory Safety Standard. If the SOP instructions are only included in your protocols please make reference as to their location in the Chemical Hygiene Plan (CHP). These examples are based on a general lab use of the chemicals listed. Procedures in your lab may require extra precautions based on how the chemical is used, e.g., hot or pressurized processes, unusually large quantities, mixtures or reactions with other chemicals, etc.. Acrylamide: neurotoxin, suspected human carcinogen. Hazards: Highly toxic and irritant (skin, eyes, respiratory tract), causes CNS paralysis. Routes: Toxic by inhalation and skin contact. Absorbed through unbroken skin. Protection: Use premixed gel whenever possible. Weigh out dry material in a fume hood, wear latex gloves, safety glasses and lab coat when handling, thoroughly wash hands when finished. Take care not to create dust. Chloroform: chlorinated solvent, suspected human carcinogen. Hazards: Very volatile. Causes CNS depression and dermatitis, forms hazardous gases when mixed with acids. Routes: Toxic by inhalation and skin contact. Protection: Always use in fume hood, wear Polyvinyl Alcohol (PVA) gloves, safety glasses and lab coat when handling. Do not mix or store with acids, may form toxic gas. Thoroughly wash hands when finished handling. Diethyl Pyrocarbonate: Hazards: Toxic and irritant (skin, eyes, respiratory tract), causes CNS paralysis. Refrigerate, moisture sensitive, may build pressure in storage. Do not use past expiration date, date upon opening. Routes: Toxic by inhalation and skin contact. Protection: Wear rubber gloves, safety glasses and lab coat when handling, open carefully in fume hood. Thoroughly wash hands when finished. Dimethyl Dichlorosilane: Hazards: Highly toxic, flammable. Causes burns, reacts violently with water. Routes: Toxic by inhalation and skin contact. Protection: Use in fume hood, wear heavy rubber gloves, safety glasses and lab coat when handling. Keep away from water and ignition sources. Thoroughly wash hands when finished handling. Ether: Hazards: Causes narcosis and irritation (skin, eyes, respiratory tract). Very volatile and extremely flammable; do not use near ignition sources. Forms explosive peroxides. Heat and light speed peroxide formation. Can form peroxides in less than 12-months. Routes: Toxic by inhalation. Protection: Use in fume hood, wear Neoprene gloves, safety glasses and lab coat when handling. Do not use or store near an ignition source, heat or oxidizers. Date upon opening, do not use past expiration date. Do not distill to dryness. Thoroughly wash hands when finished handling. Ethidium Bromide: Toxic, mutagen. Hazards: Irritating to eyes, respiratory system and skin. May cause heritable genetic damage. Routes: Toxic by inhalation, ingestion, and skin contact. Protection: Use in fume hood, wear latex gloves, safety glasses, and lab coat. Thoroughly wash hands when finished. Formaldehyde: Suspected human carcinogen. Hazards: Combustible. Highly irritating to mucous membranes. Carcinogen. Routes: Toxic by inhalation and readily absorbed through skin. Protection: Use in a fume hood, wear Nitrile gloves, safety glasses and lab coat when handling. Thoroughly wash hands when finished. Halogenated Anesthetic Gases Hazards: Highly volatile, CNS depressant. Routes: Toxic by inhalation. Protection: firmly attach anesthetic delivery system to subject. Use in a well ventilated area, e.g., fume hood, canopy hood. Use scavenger exhaust system and make sure tracheal bubble is inflated during use. Nitric Acid (70%): Strong acid. Hazards: Strong oxidizer, causes burns, ignites combustibles, and reacts violently with water and organics. Routes: Highly toxic by inhalation and contact with skin or eyes. Protection: Use in fume hood, wear Neoprene gloves, safety goggles, and a protective apron when handling, thoroughly wash hands when finished. Do not store with flammables or combustibles. Osmium Tetroxide: Toxic. Hazards: Highly toxic, vapor poisonous, irritant (skin, eyes, respiratory tract), causes damage to eyes respiratory tract and skin, keep away from combustible materials. Routes: Toxic by inhalation and skin contact. Protection: Always use in a fume hood, wear latex gloves, safety glasses and lab coat when handling, thoroughly wash hands when finished. Take care not to create dust. Tetrahydrofuran: Hazards: Volatile, flammable do not use near ignition sources. Forms explosive peroxides. Date upon opening, and do not use past expiration date. Do not distill to dryness. Irritating to eyes, respiratory system and skin. Routes: Inhalation Protection: Test for peroxide formation periodically and before distillation. Use and distill only in a fume hood. Wear Viton gloves, safety glasses and lab coat when handling, thoroughly wash hands when finished. Check that water is running during distillation and do not let process run over night. Phenol: Hazards: Corrosive, highly toxic and combustible. Readily absorbed through the skin. Ingestion of even small amounts may cause nausea, vomiting, circulatory collapse, convulsions, coma, death, etc. Routes: Highly toxic through inhalation and ingestion and readily absorbed through the skin. Protection: Always use in a fume hood, wear Butyl rubber gloves, lab coat and safety glasses. When working with large concentrated volumes wear goggles and a protective apron. Thoroughly wash hands when finished.