CHP Notebook
Table of Contents
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6
Section 7
Section 8
Section 9
Section 10
Section 11
Section 12
Section 13
Section 14
Safety Program Key Personnel
Laboratory Room Assignments
Laboratory-Specific Information
Emergency Phone Numbers & Procedures
Chemical Hazard Assessment (List)
Material Safety Data Sheets
Laboratory-Specific Standard Operating Procedures
Record of Training for Laboratory Personnel
Inspections and Exposure Monitoring
Accidents, Injuries, and Corrective Actions
Disposal of Waste Materials
Radiological Hazards
Lab and Building Specific Evacuation Information
Housekeeping and Maintenance Inspections
Section 1
Safety Program Key Personnel
In addition to the following personnel, please include your department's safety program key
personnel.
Name
Laura Drabcyk
Position
EHS Director, Environment, Health & Safety
Phone
593-4914
Lou Blatnik
EHS Specialist, Environment, Health & Safety
593-0921
Richard Gordon
Director, Public Safety
593-1908
Ken Paris
Lieutenant, Public Safety
593-5337
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Section 2
Laboratory Room Assignments
This Chemical Hygiene Notebook pertains to the laboratories and the research facilities of:
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List all rooms related to the above mentioned lab/research facility. Include all rooms such
as temperature controlled, storage closets, and animal facilities. Indicate the faculty who
currently direct the indicated rooms.
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Section 3
Laboratory-Specific Information
Each research group should develop its own listings of rooms and personnel. There is no
required format; the following can de used as a guide.
Name of Principle Investigator: ___________________________________________
Name of Lab Chemical Hygiene Officer: ___________________________________
Lab/Room Number(s): _________________________________________________
Lab Phone Number(s): _________________________________________________
Names and titles of other lab employees and users:
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Section 4
Emergency Phone Numbers and Procedures
Each laboratory group should develop plans and procedures for dealing with emergency
situations. As a minimum, this should include phone numbers and evacuation plans. Also
refer to the "Emergencies" section of the CHP for additional information.
Name of Principle Investigatior (PI):
Emergency Phone Number(s):
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______________________________
______________________________
Name of Lab CHO (if different than PI):
Emergency Phone Number(s):
________________________________
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Names of Other Lab Personnel:
Emergency Phone Number:
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Section 5
Chemical Hazard Assessment (List)
Each laboratory is responsible for taking and maintaining its own chemical list. A copy of
the current inventory is to be placed in this section.
A Chemical List should have a clear title and heading, which includes the following
information:
•
•
•
•
Room number and building name
Department
Name of person taking inventory
Date of inventory
There is no specific required format for the information provided in the Chemical List. Lab
supervisors should also consider their own needs for chemical management. The inventory
should strive to identify the following :
•
•
•
•
•
•
Chemical name
Location
Basic hazard information or classification
Approximate quantity
If chemical is reordered
If quantity changes significantly
EHS recommends the following column headings:
Quantity Chemical Name Hazard Class Location Date Received Date Removed
The Hazard Classifications (possibly more then one per chemical) can be identified with
notations such as the following notations (suggested by EHS):
A
Compressed Gas
G
Corrosive
B
Severe Poison
H
Radioactive
C
Moderate Poison
I
Biohazard
D
Water Reactive
J
Carcinogenic
E
Oxidizer
K
Non-Hazardous
F
Flammable
L
"Other” notations
It is the responsibility of the Principle Investigator to determine if chemicals in use or in
storage produce a potential hazard which must be identified on the Chemical List.
Section 5 -- Chemical Hazard Assessment (List) (cont’d)
Quantity
(include unit of
measure)
Chemical Name
Hazard
Class(es)
Location
Date
Received
Date
Removed
Section 6
Material Safety Data Sheets (MSDS)
This section should include the location of MSDS for any special materials or particularly
hazardous chemicals used in this laboratory. Each lab is responsible for obtaining and
maintaining copies of relevant MSDS. Most MSDS can quickly be found through the lings
below or a google search. The primary database of MSDS’s for the UM-Dearborn is
maintained by Natural Sciences. Each laboratory shall keep a separate MSDS Notebook
specific to their area and is to be kept in a specified location (noted below).
The MSDS Notebook is located: ____________________________________________.
The following links to MSDS Databases are available:
EMD Chemicals:
http://www.emdchemicals.com/?RedirectedFrom=http://www.emdchemicals.co
m/analytics/EMD_Analytics.asp
Fisher Scientific:
https://www.fishersci.com/wps/portal/CMSTATIC?pagename=msds/
Household Products Database:
http://hpd.nlm.nih.gov/
Linde Group:
http://msds.lindeus.com/
Mallinckrodt Baker:
http://www.mallbaker.com/changecountry.asp?back=/Default.asp
Public Health Agency of Canada (Infectious Substances):
http://www.phac-aspc.gc.ca/msds-ftss/
Sigma-Aldrich
http://www.sigmaaldrich.com/sigmaaldrich/errorpage.html?status=404&r=http%3A%2F%2Fwww.sigmaaldrich.co
m%3A4402%2Fsite-level%2Fmsds.html%2F
Vermont SIRI Web Page:
http://siri.org/msds/index.php
VWR Scientific Products:
https://www.vwrsp.com/search/index.cgi?tmpl=msds/
Section 7
Laboratory-Specific Standard Operating Procedures (SOP)
Each laboratory working with hazardous chemicals should include their own specific
Standard Operating Procedures (SOP) here. This section can include laboratory-specific
procedures for the following subjects, as they apply for each laboratory, and many other
additional topics. A General SOP Template and a Laser SOP Template are available for
your use. For additional help, see SOP Examples
 Chemical procurement, distribution, and storage
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Working with carcinogens or reproductive toxins
Housekeeping, maintenance, and inspections
Protective apparel and equipment
Dealing with hazardous procedures, substances, and/or equipment
Working with radioisotopes
Working with biologically hazardous materials
Operations requiring special prior approval
Any additional special equipment, experimental procedures, or unique hazards
A General SOP Template, Particularly Hazardous Materials SOP Template, Laser SOP
Template and a Compressed Gas SOP are available for your use. For additional help, refer
to the SOP Examples that follow this section.
LABORATORY STANDARD OPERATING PROCEDURE
(for the use of hazardous materials or equipment)
NAME OF PROCEDURE:
PREPARED BY:
REVISION DATE:
LOCATION – This procedure may be performed at the following location(s):
HAZARDS – The materials and equipment associated with this procedure present the
following exposure or physical health hazards. Safety precautions are prudent and
mandatory:
ENGINEERING CONTROLS – Prior to performing this procedure, the following
safety equipment must be accessible and ready for use, e.g., chemical fume hood,
biological safety cabinet, laminar flow hood, chemical spill kits, etc.
PROTECTIVE EQUIPMENT – Prior to performing this procedure, the following
personal protective equipment must be obtained and ready for use, e.g., acid resistant
gloves, safety eyewear, lab coat, chemical splash apron, etc.
WASTE DISPOSAL – This procedure will result in the follow regulated waste which
must be disposed of in compliance with environmental regulations:
ACCIDENTAL SPILL – In the event that a hazardous material spills during this
procedure, be prepared to execute the following emergency procedure:
Call DPS at 9-1-1 from a campus phone (if after hours)
PRIOR APPROVAL – This procedure is considered hazardous enough to warrant
prior approval from the Principal Investigator.
- YES - NO CERTIFICATION – I have read and understand the above SOP. I agree to contact
my Supervisor or Lab manager if I plan to modify this procedure.
Signature
Name (Print)
Date
Room #
< Date this was drafted >
< The SOP Title Here >
Standard Operating Procedure
< Your Name Here >
Write a brief description of what this process is used for here.
1.0 Material Requirements:
1.1 Equipment needed for process:
List all of the equipment you need to successfully run this process. Be complete. If
this is a new process that has not been done in the lab before, consult with lab staff to
make sure that equipment you need is available.
1.2 Chemicals needed for process:
Include a complete list of the chemicals needed. If specific concentrations, purities,
or grades are necessary cite them here.
1.2.1 Hazards associated with chemicals:
Include a subsection for each component chemical and if necessary a
subsection for the chemical produced. Hazards will be found in the MSDS.
Look for information on whether the chemical is flammable, corrosive, toxic,
carcinogenic, pyrophoric, an irritant, etc.
1.3 Engineering controls:
Where will you run this process? If this process is to be run in a specific location in
the lab be explicit. Options include fume hoods, vented ovens, furnaces, glove
boxes, wet benches. If this is a new process and the appropriate engineering controls
do not seem to be available in the lab, discuss with lab staff whether the process can
be done and how to obtain what is needed. If no engineering controls are needed
please cite this fact. Some of this information will be in the MSDS.
1.4 Protective equipment needed:
What do you wear to protect yourself while performing the process? Check MSDS,
but be aware that the protective equipment standards cited in these documents
frequently refer to handling the chemical outside of approved engineering controls.
If you do not know what protective equipment to use or what types of gloves are
compatible with the chemicals you will be using, consult lab staff.
2.0 Procedure:
Include detailed instructions on mixing the component chemicals and or use of the gas.
Information you should cite will include temperature settings, flow rates/pressure,
concentrations, volumes and weights, appearance (if applicable), what the process is
supposed to do, how to tell if the process was successful, and what to do with the hardware,
chemicals, and equipment after the process is completed.
3.0 Storage:
Where will you store this in the lab, e.g., solvent, acid, or base cabinet, refrigerator, etc.? Be
aware of incompatibility with other chemicals already in use in the lab. For example, one
chemical might react violently with another. In this case you would want to avoid storage in
a cabinet with this second chemical. Information will be found in the MSDS.
4.0 Waste Products:
How do you dispose of the waste products used in making the material, after using the
material or after the material is depleted or gets old. Be specific and describe the specific
disposal procedure to be used within the lab, i.e., do not write “Dispose of in accordance
with applicable regulations.” Ask members of the laboratory staff for assistance if you do
not know how the waste chemical should be handled.
5.0 Incident Procedures: (Found in the MSDS)
5.1
Contact (include a subsection for each component chemical)
5.1.1 Skin:
5.1.2 Eyes:
5.1.3 Inhalation:
5.1.4 Ingestion:
5.2
Spill or leak: How do you deal with a small spill? Are there specific
absorbents that should be used? Is the disposal procedure you cited in
section 4 valid in case of a spill? If it’s a gas leak, what do you do when the
detectors go off? Be specific. Do not include the chemical manufacturer’s
emergency numbers here.
5.3
Fire: Are there specific concerns to be considered in the event of a fire?
For example, some chemicals are water reactive, and using water on a fire
where these chemicals are involved will make the problem worse.
For emergencies during non-business hours, call the Dept. of Public Safety at 91-1 (from any campus phone) or 313-593-5333.
LABORATORY STANDARD OPERATING PROCEDURE (SOP)
FOR THE USE OF
PARTICULARLY HAZARDOUS SUBSTANCES PAGE 1
This template SOP is designed to provide guidance in writing procedures for the safe handling and
disposal of particularly hazardous substances including carcinogens, engineered nanomaterials,
hazardous drugs and toxins. This template must be customized and reviewed for its applicability to
the specific procedure listed below. See Section 8.5 of the Chemical Hygiene Plan (Materials and
Procedures Requiring Special Provisions) for detailed information under what conditions a specific
SOP is required. If you have questions concerning the applicability of any item listed in this
procedure contact the Principal Investigator/Laboratory Supervisor or Environment, Health & Safety
313-593-4914.
TABLE OF
CONTENTS:
Section
Page
Procedure Title, Description, Preparer, Revision Date
Location, Designated Area
Hazards
Control of Hazards
Protective Equipment
Decontamination Procedures
Special Handling and Storage
Waste Disposal
Accidental Spill
Training Requirements
Prior Approval, Medical Surveillance determination
Certification of Training
Attachments
Appendix A
Animal Handling Procedures Supplement
1
1
2
2
3
4
4
4
4
5
5
6
7
SPECIFIC PROCEDURE TITLE:
PREPARED BY:
REVISION DATE:
DESCRIPTION OF PROCEDURE:
LOCATION: These procedures must be performed in a designated area.
Describe location(s):
________________________________________________________________________
A designated area shall be established where limited access, special procedures, knowledge,
and work skills are required. A designated area can be the entire laboratory, a specific
laboratory workbench, or a laboratory hood. Designated areas must be clearly marked with
PARTICULARLY HAZARDOUS SUBSTANCES SOP
PAGE 2
signs that identify the hazard and include an appropriate warning; for example: WARNING!
FORMALDEHYDE WORK AREA – CARCINOGEN.
 Upon leaving the designated area, remove any personal protective equipment worn and
wash hands, forearms, face, and neck.
 After each use, wipe down the immediate work area and equipment to prevent
accumulation of chemical residue.
 At the end of each project, thoroughly decontaminate the designated area before
resuming normal laboratory work in the area.
Storage or consumption of food, storage or use of containers of beverages, storage or
application of cosmetics, smoking, storage of smoking materials, tobacco products or other
products for chewing, or the chewing of such products are prohibited in designated areas.
Designated areas shall be maintained under negative pressure with respect to corridors and
public areas.
HAZARDS: The chemicals associated with this procedure present exposure or physical
health hazards. Safety precautions are prudent and mandatory. The MSDSs for the
chemicals used in the area must be accessible to lab personnel. MSDS location:
______________________________________________________________.
Select the hazard class: (circle all that apply) Carcinogen, chemotherapeutic agent,
engineered nanomaterial, mutagen, embryotoxin, highly toxic, sensitizer. Other: (describe)
___________________________________.
Route of exposure: (circle all that apply) Inhalation, contact, ingestion, injection.
Major target organs: (circle all that apply) Skin, liver, lung, kidney, reproductive, CNS.
Physical chemical properties: (circle all that apply) Flammable, explosive, reactive,
corrosive, peroxide forming.
Add specific chemical hazard information here:
Example: Long-term risk of occupational exposure to cytotoxic (Chemotherapeutic)
hazardous drugs (HD) has been associated with human cancers at high (therapeutic) levels
of exposure. These drugs have been shown to be carcinogens, mutagens and teratogens in
many animal species. There is evidence that hazardous drugs may cause spontaneous
abortions and increase the risk of congenital malformations. In addition, some of these
drugs have been shown to cause acute effects in humans, such as localized skin necrosis
(death of tissue) damage to normal skin after surface contact, dizziness, lightheadedness.
Adverse health effects from both acute and chronic exposures have been demonstrated in
health care personnel. All of these drugs have potential to damage cells or adversely affect
cellular growth and reproduction. The drugs bind directly to genetic material in the cell
nucleus, or affect cellular protein synthesis. Employees can be exposed to hazardous drugs
PARTICULARLY HAZARDOUS SUBSTANCES SOP
PAGE 3
through inhalation of drug dust or droplets, absorption through the skin directly, injection
through the skin or ingestion through contaminated food.
CONTROL OF HAZARDS: Methods used to control hazards will rely on engineering
controls over administrative controls and personal protective equipment.
Type of engineering control utilized: (circle) Fume hood, negative pressure glove box,
sealed system, Class II Biological Safety Cabinet (BSC), other local exhaust system.
Indicate specific hazard controls that must be utilized to perform this procedure.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
_______________________________________________
Additional required procedures:
Although the specific SOPs will vary according to the material used, the following
guidelines are generally applicable for projects involving particularly hazardous substances:
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Use the smallest amount of chemical that is consistent with the requirements of the work
to be performed.
Use containment devices (such as lab fume hoods or glove boxes) when: (i) volatilizing
these substances, (ii) manipulating substances that may generate aerosols, and (iii)
performing laboratory procedures that may result in uncontrolled release of the
substance.
Use high efficiency particulate air (HEPA) filters, carbon filters, or scrubber systems
with containment devices to protect effluent and vacuum lines, pumps, and the
environment whenever feasible.
Use ventilated containment to weigh out solid chemicals. Alternatively, the tare method
can be used to prevent inhalation of the chemical. While working in a laboratory hood,
the chemical is added to a pre-weighed container. The container is then sealed and can
be re-weighed outside of the hood. If chemical needs to be added or removed, this
manipulation is carried out in the hood. In this manner, all open chemical handling is
conducted in the laboratory hood.
Use containment devices for the following procedures that may also present
opportunities for employee exposure during preparation: Withdrawal of needles from
drug vials; Drug transfers using syringes and needles; Breaking open ampoules;
Expulsion of air from drug-filled syringe.
If the process does not permit the handling of such materials in an OSEH certified
ventilation control device, contact EHS at 313-593-4914 to review the adequacy of control
measures.
PARTICULARLY HAZARDOUS SUBSTANCES SOP
PAGE 4
PROTECTIVE EQUIPMENT: Prior to performing this procedure, the following
personal protective equipment must be obtained and ready for use: (ex. acid resistant
gloves, safety eyewear, lab coat, chemical splash apron):
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Hand Protection - Select appropriate glove type and double glove. For proper selection of
glove material, review chemical MSDS and glove manufacturer’s glove selection guides
(see OSEH web site for links).
Glove Type: (circle appropriate glove material) nitrile, neoprene, vinyl, latex, laminate.
Gloves should be changed frequently and should be pulled up over outer sleeves to reduce
skin exposure.
Where to Find Compatibility Information
Most glove manufacturers have chemical compatibility charts available for their gloves.
These charts may be found in laboratory supply catalogs such as Fisher Scientific and Lab
Safety Supply. Best Gloves. Best Gloves also offers an excellent on-line database for glove
selection at www.bestglove.com.
Eye Protection – Select appropriate eye protection.
Eyewear: (circle appropriate type) safety glasses, Safety goggles
Goggles (not safety glasses) are appropriate for processes where splash, spray, or
aeresolization is foreseeable.
Other Protective Clothing
At minimum, lab coat, long pants, and closed toed shoes are to be worn when entering
laboratories having hazardous chemicals.
Hazardous chemicals that are toxic via skin contact/absorption may require additional
protective clothing (i.e., face shield, apron, oversleeves, bonnets) as appropriate where
chemical contact with the body/skin is foreseeable.
Respiratory protection – Is not required when proper engineering controls are
implemented. In some cases a N95 disposable respirator can be used as an added layer of
protection from particulates or a potential splash. Respirator usage may require training, fit
testing and a medical evaluation. Contact OSEH (7-1143) to determine requirements for
specific applications.
PARTICULARLY HAZARDOUS SUBSTANCES SOP
PAGE 5
DECONTAMINATION PROCEDURES
Personnel decontamination: Immediately after working with particularly hazardous
materials, remove gloves, wash hands and arms with soap and water. Any time you leave
the designated area you must remove protective clothing and conduct personnel
decontamination.
Area decontamination: Decontamination procedures vary depending on the material being
handled; consult the MSDS. Consideration should be given to neutralizing some agents
with other reagents as part of the decontamination process; consult the MSDS, EHS, and
other resources to determine applicable methods.
All surfaces should be wiped with the appropriate cleaning agent following dispensing or
handling. Waste materials generated should be treated as a hazardous waste.
In the absence of other methods, decontamination should consist of surface cleaning with
water and detergent followed by thorough rinsing. The use of detergent is recommended
because there is no single accepted method of chemical deactivation for all agents involved.
A plastic backed absorbent pad should be placed under the work area during the process.
This should be changed at the end of each process or when a spill occurs.
Equipment decontamination: Decontaminate glassware, vacuum pumps or other
contaminated equipment before removing from the designated area. Attach the EHS
Equipment Decontamination form to equipment to be sent offsite.
Biological Safety Cabinets used for antineoplastic preparation should be cleaned daily with
70% ethanol solution and decontaminated weekly and whenever spills occur.
Decontamination procedures should include surface cleaning with high pH agents; thorough
rinsing, removal, and cleansing of work trays; and sump cleansing.
SPECIAL HANDLING AND STORAGE
Note storage
location:__________________________________________________________________
Label containers and storage areas with strong warnings like "CANCER-SUSPECT
AGENT".
Ensure secondary containment and segregation of incompatible chemicals per guidance
within the Chemical Hygiene Plan. Follow any substance-specific storage guidance
provided in MSDS documentation.
PARTICULARLY HAZARDOUS SUBSTANCES SOP
PAGE 6
WASTE DISPOSAL - This procedure will result in the following regulated waste which
must
be
disposed
of
in
compliance
with
environmental
regulations:
____________________________________________________
All particularly hazardous substance waste including gloves, syringes, vials, and solution
containers should be placed in a labeled 5 gallon white pail. Needle syringe assemblies
must be disposed in sharps containers with hazardous waste labels. Needles must not be
recapped for disposal. The waste container must and be located within the designated area
and should be closed except when actively adding waste. Contact EHS at 313-593-4914 for
supplies and to schedule removal of waste.
ACCIDENTAL/INCIDENTAL SPILL: Prompt response to chemical spills is critical to
protect worker health and safety and to mitigate adverse affects to the environment. Spills
should be identified with a warning sign to limit access to the area until decontamination has
been completed. Spills should be cleaned up immediately by a properly protected employee
who has been trained in the appropriate procedures regarding the handling and disposal of
hazardous substances. Spills should be cleaned with a mild detergent and rinsed twice with
water.
All contaminated cleanup materials should be disposed of in the appropriately labeled waste
container. Call EHS at 313-593-4914 for disposal of contaminated waste materials and for
assistance in cleaning the spill.
In case of a spill onto employee's skin or eyes, quick response to the nearest emergency
shower and eyewash location is necessary to reduce exposure. Remove contaminated
clothing and gloves. Don clean gloves and wash the affected skin area with soap and water.
For eye contact, flush the eye with water for 15 minutes and seek medical attention.
Employees should be referred to Midwest Health Service, the UM –Dearborn Occupational
Health Provider, for treatment. Any exposure must be reported on the WorkConnections
Injury and Illness form and sent to WorkConnections.
Spills of powdered material should be cleaned up by personnel wearing appropriate
respiratory protection, double protective gloves and gowns. Wet towels should be placed
over the spilled material, and water should be used to absorb any dry powder. Materials
used in clean-up should be placed in appropriate waste barrels and treated as hazardous
waste.
Laboratory personnel who work with hazardous chemicals are to be provided the
opportunity to receive medical attention/consultation when:
 A spill, leak, explosion or other occurrence results in a hazardous exposure (potential
overexposure).
 Symptoms or signs of exposure to a hazardous chemical develop.
Specific emergency procedures shall be posted and employees shall be familiar with them.
PARTICULARLY HAZARDOUS SUBSTANCES SOP
PAGE 7
TRAINING REQUIREMENTS
EHS general laboratory safety training as well as laboratory-specific training is required.
Each employee, prior to being authorized to enter a designated area, shall receive training
from the PI or an experienced lab manager that includes but not necessarily limited to:

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





The nature of the hazard, including local and systemic toxicity;
The specific nature of the operation that could result in exposure;
The purpose and application of the medical surveillance program, including, as
appropriate, methods of self-examination;
The purpose and application of decontamination practices and purposes;
The purpose and significance of emergency practices and procedures;
The employee's specific role in emergency procedures;
Specific information to aid the employee in recognition and evaluation of conditions and
situations which may result in the release of chemicals addressed by this section;
The purpose and application of specific first aid procedures and practices;
PRIOR APPROVAL: This procedure is considered hazardous enough to warrant
prior approval from the laboratory director.
-YES-NOMEDICAL SURVEILLANCE: This procedure requires medical surveillance through
the EHS Medical Surveillance program (contact EHS at 313-593-4914 for assistance on
a determination of medical surveillance).
-YES-NOCERTIFICATION: I have read and understand the above SOP. I agree to contact my
supervisor or lab director if I plan to modify this procedure.
Signature
Name (Print)
Date
Room #
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
COMPRESSED GAS CYLINDER SOP TEMPLATE
< Date this was drafted >
Compressed Gas Cylinder Handling and Use
Standard Operating Procedure
< Your name here >
1.0 Material Requirements:
1.1 Equipment needed:
List all of the equipment you need. Be complete. Include cylinder cart, bubble soap,
helium leak check device, etc.
1.2 Gases Used in < XXXXXX > Lab:
Include a complete list of the gases used by your research group. Specify
concentrations, purities, or mixtures.
1.2.1 Hazards associated with gases:
Include a subsection for each gas. Hazards will be found in the MSDS. Look for
information on whether the gas is flammable, corrosive, toxic, carcinogenic,
pyrophoric, an irritant, etc.
1.3 Engineering controls:
Options include gas cabinets, local exhaust ventilation, cylinder storage racks, bench
mounted restraints, etc. Compressed gas cylinders must be secured in an upright position
away from excessive heat, highly combustible materials, and areas where they might be
damaged or knocked over.
Be specific as to location where the gases will be used, especially if this SOP applies to
more than one laboratory in your research group.
This section must also include information on what tubing material is or is not allowed
and information on regulator selection and use. Never interchange regulators and hose
lines (with one type of gas for another). Explosions can occur if flammable gases or
organic materials come in contact with oxidizers (e.g., oxygen) under pressure.
1.4 Protective equipment needed:
What do you wear to protect yourself while changing the cylinders or using the gases?
Check MSDS, but be aware that the protective equipment standards cited in these
documents frequently refer to handling the gas outside of approved engineering controls.
If you do not know what protective equipment to use, consult EHS.
2.0 Procedure:
Include detailed instructions on transporting the cylinders, securing the cylinders, changing the
cylinders, attaching tubing and regulators, leak-check procedures and use of the gas.
Information you should cite will include appropriate flow rates/pressure, concentrations,
volumes and weights (if applicable), how to tell if the cylinder is leaking, and what to do after
the process is completed.
NOTE:
If you have a gas cabinet and/or gas detection system you will probably want to have
a separate SOP for operation and maintenance of those systems.
3.0 Storage:
Where will you store the cylinders in the lab? Cylinders must be stored in dry, wellventilated areas. Closets and lockers would not be acceptable storage locations. Cylinders
must be stored with the protective caps in place.
How much gas do you intend to keep on hand? Under no circumstances should a researcher
purchase more than a 4-year supply (the normal course of a research project).
Be aware of incompatibility with other gases already in use in the lab. For example,
cylinders of oxygen and other oxidizers must be stored at least 20-feet from fuel-gas or other
combustible materials unless separated by a noncombustible wall, not less than 5-feet high,
having a fire-resistance rating of ½-hour. Information will be found in the MSDS.
4.0 Empty Cylinders:
At what point are cylinders considered “empty”, and where do they go when they are empty.
Be specific and describe the cylinder return procedure to be used within the lab, i.e., do not
write “Dispose of in accordance with applicable regulations” or “Return to storage area for
pickup by supplier.”
5.0 Accident Procedures: (Found in the MSDS)
5.1
5.2
5.3
Contact (include a subsection for each gas)
5.1.1 Skin:
5.1.2 Eyes:
5.1.3 Inhalation:
5.1.4 Ingestion:
Gas leak: How do you deal with a small leak? A larger leak? Be specific
with regard to UM procedures and phone numbers. Do not simply include
the gas supplier’s emergency numbers here. If your lab has a gas alarm,
indicate what procedures must be followed when the alarm sounds.
Fire: Are there specific concerns to be considered in the event of a fire? For
example, some chemicals are water reactive, and using water on a fire where
these chemicals are involved will make the problem worse.
Include the following information:
Report all accidents, e.g., injuries, leaks, fires, etc., to the . . . (fill in, as
applicable for your research group). This could be the Principal Investigator,
Laboratory Director, etc. You will also need to submit an incident report to the
Public Safety
For emergencies during non-business hours, call Public Safety at 9-1-1 (from
any campus phone) or (313-593-5333.
Section 8
Record of Training for Laboratory Personnel
Records of training sessions attended by each person using the laboratory (including the
supervisor) should be kept in this section. Records should include the laboratory user’s
name, the type of training (e.g., Lab Safety Lecture, Lab-Specific Training, etc.), who
conducted the training (lab supervisor, etc.), and the date of the session. Records of training
or training certificates provided by EHS should also be added to this section. Two different
forms are provided to document lab specific training. Both forms are useful and may be
used as needed. A training checklist is also included to cover labsafety basics as well as
specific training. This checklist can be used as a guide to topics that should be covered or as
an actual training document that is completed for each employee. If used as a training
record it should be signed and dated.
CHEMICAL HYGIENE TRAINING FORM
Name & UMD ID #
Type of Training
Conducted By
University of Michigan-Dearborn
Date
Certification of Personal Protective Equipment Training
I,
, certify that the following affected employees
(print full name)
have received and understood personal protective equipment (PPE) training, which included
the following: when PPE is necessary; what PPE is necessary; how to properly don, doff,
adjust, and wear PPE; the limitations of the PPE; and the proper care, maintenance, useful
life and disposal of the PPE. Each of the affected employees has demonstrated an
understanding of the above and an ability to use the PPE properly. This training is in
compliance with 29 CFR 1910.132 (f) and MIOSHA Part 33, Rule 408.13300.
Name
UM ID
Number
Date of
Training
Equipment Type
(signature)
(date)
Note to signer: maintain this certification with your permanent records.
EMPLOYEE SPECIFIC CHEMICAL HYGIENE TRAINING FORM
Name ___________________________________________
Department ______________________________________
Campus Location __________________________________
Campus Phone Number______________________________
Employee Title _____________________________________
Supervisor _________________________________________
OSHA's Laboratory Standard (29CFR1910.1450) and MIOSHA Part 431, Rule 325.70100,
requires that each laboratory employee be made aware of the location and content of the
laboratory's Chemical Hygiene Plan. By your signature below, you acknowledge that you
have read and understood the contents of this plan and know its location within the
laboratory.
____________________________________________
____________________
Employee Signature
Date
The Laboratory Safety Standard further requires that the employee's supervisor
provide training, which covers the specific topics described in the "Information and
Training" section of the Chemical Hygiene Plan. This training must be provided at the
time of the employee's initial assignment, on a refresher basis at least annually and
upon updating procedures. Document specific training below:
Description of Training
Date
Training Checklist
Provided By
This checklist may be used to assist with the laboratory specific
training requirements outlined in the Laboratory Safety Standard.
Introduction to laboratory specific Chemical Hygiene Plan (CHP):
Location and contents
Introduction to laboratory specific Standard Operating Procedures
(SOP’s)
Review Chemical Inventory
Review Location of MSDS’s
Review emergency information: Spills, Personal Injury, Fire, Power
Failure
Fire extinguisher
First Aid Supplies
Evacuation Plans
Basic Safety Rules
Note rules with special importance for your laboratory.
Identify specific areas for food consumption.
Review procedures for working after hours.
Review the Chemical Inventory for your laboratory
Review the UM-Dearborn Waste Handling Procedures
Labeling
Packaging
Pick-ups
Review procedures for chemical procurement, distribution, and
storage
Review Standard Operation Procedures for use of toxic materials
Storage (acid cabinet, flammable liquid storage cabinet, flammable liquid
storage refrigerator, etc.)
Personal Protective Equipment (PPE)
Location of procedure (mechanical ventilation required)
Waste Disposal (aqueous, solid, biohazardous, radioactive)
Review Procedures for Use of Compressed Gas Cylinders
Protective Apparel and Equipment
Discuss when safety glasses, goggles, or face shields are required.
Discuss any need for other protective equipment
Discuss selection of gloves.
Housekeeping, Maintenance, and Inspections
Discuss materials stored or frequently present on the floor.
Discuss maintenance items for scientific equipment.
Discuss formal and internal inspection programs.
Environmental Monitoring
Discuss PEL's and TLV's for chemicals in use and how to reduce employee
exposure.
Discuss building ventilation.
Discuss use of fume hoods, biological safety cabinets or other mechanical
ventilation systems.
Review SOP for working with biologically hazardous materials
Review Exposure Control Plan if working with human blood or other
potentially infectious materials.
Discuss Biosafety Manual if working with recombinant DNA or infectious
agents.
Review Hepatitis B Vaccination Program.
Review PPE, Housekeeping and Waste Disposal Procedures.
Working with Radioisotopes
Review radiological safety practices
Review Dosimetry Program
Medical Program
Discuss need for any medical surveillance (Respirator use, Potentially
Hazardous Materials, Heat Stress, Noise, Animal Handler, Laser Operator,
etc…) contact EHS (313-593-4914) for assistance.
Training Program
Discuss Departmental Specific and EHS training sessions.
Additional Safety Session Topics
Review recent incidents/accidents/injuries and how to prevent reoccurrence.
Review new equipment at least annually.
Review new procedures at least annually.
Review results of recent inspections and how to correct problem areas.
Section 9
Inspection and Exposure Monitoring Records
This section should contain information on laboratory inspections and exposure monitoring
(including the date and recommendations). Inspections may be conducted by individual
research groups, EHS, MIOSHA, NRC, and possibly other organizations. This section
should also contain records associated with corrective actions. Please refer to one of the
following for your laboratory: Laboratory Safety Checklist, Machine Shop Safety
Inspection, or the Laser Safety Inventory & Inspection Sheet.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Section 10
Housekeeping and Maintenance Inspections
Periodic housekeeping and maintenance self-inspections are recommended. The inspections
should include equipment, structural integrity, and personal protective equipment. This
section should note who is responsible for this inspection, how often inspections will be
conducted and where the records are kept. The table below can be utilized for maintenance
inspections. If inspections are conducted using OSEH’s Laboratory Safety Checklist,
this will satisfy the housekeeping requirement because housekeeping is one of the items on
this checklist.
Inspector:
Date of Inspection:
Location of Records:
Equipment
Satisfactory
Semi-annual Laboratory Safety Checklist
Unsatisfactory
Building:
Comments / Action
Rooms:
Lab Manager:
Inspector:
Date:
Yes / No / NA
Written Materials:
Door signs posted with contact personnel:
Emergency numbers posted by telephone:
Chemical hygiene plan with SOPs present:
Personnel aware of CHP & ECP Training:
Chemical inventory for all chemicals:
Personal protective equipment present and used:
Laboratory coats:
Gloves (Disposable & Chemical):
Safety glasses/goggles/face shield:
Other (apron, respirator, ear plugs, etc):
Safety equipment present and in working condition:
Emergency shower & inspection date current:
Emergency eye-wash & inspection date current:
Drench hose:
Fire extinguisher & inspection date current:
Fumehood & inspection date current:
Biological safety cabinet & inspection date current:
Spill kits readily available:
Chemical Storage:
All containers of chemicals are labeled:
Gas cylinders are secured and capped:
Chemicals are stored on shelves/in cabinets:
Chemicals are stored by class:
Large containers are on low shelves:
Electrical Hazards:
Extension cords are prohibited:
Outlet extenders are prohibited:
Electrical cords are in good condition:
Surge suppressors/multi-outlets used properly:
General Safety:
Aisles are kept clear:
Work area is neat:
Eating/drinking/smoking prohibited in lab:
Waste Handling:
Chemical waste managed properly:
Biological waste managed properly:
Radiological waste managed properly:
Glassware/plastics/sharps managed properly:
Section 11
Accidents, Injuries, and Corrective Actions
Comments
Include in this section a description of laboratory accidents and corrective actions taken to
prevent them in the future. It is always important to take a careful look for all underlying
and precipitating causes. Copies of any EHS, MIOSHA, or other accident-related forms
should be maintained here.
Please note: An Injury or Illness Report Form must be completed for all employee
workplace injuries and illnesses go to http://www.umich.edu/~connect/forms.htm click on
“Illness and Injury Report form”. An injury or illness report form for non-employee injuries
and illnesses must be completed, go to http://www.umich.edu/~riskmgmt/forms.html click
on “Injury Report for Non-Employees”. This section should contain records pertaining to
chemical spills and medical assistance.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Section 13
Disposal of Waste Materials
Hazardous waste disposal must be coordinated with the EHS at 313-593-4914 assistance
and scheduling of a waste pick up.
Type of waste generated from area:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Person(s) responsible for waste labeling in this lab (if you need assistance EHS
at 313-593--4914):
__________________________________________________________________________
__________________________________________________________________________
List here any lab specific procedures that are not covered in your SOPs.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
_________________________________________________________________________
__________________________________________________________________________
For waste pick up call EHS at 313-593-4914.
Section 14
Radiological Hazards
Various types of records are required for regulatory compliance in all laboratories using
radioactive materials. Copies of these forms and other documents should be maintained in
this section. If maintained in the Radiation Safety Manual, include location of this manual.
If applicable please complete
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Section 14
Lab and Building Specific Evacuation Information
* For emergency information
dial 911
Laboratory Supervisor/Emergency
Coordinator for this area:
_____________________________________
Designated assembly areas:
______________________________________
______________________________________
Emergency Egress Map Example
Public Safety & Environmental Health maintains building egress maps. It is recommended
that each laboratory supervisor maintains an egress layout for their lab.
Section 13
Housekeeping and Maintenance Inspections
Periodic housekeeping and maintenance inspections are required. The inspections should
include equipment, structural integrity, and personal protective equipment. This section
should note who is responsible for this inspection, how often inspections will be conducted
and where the records are kept. The table below can be utilized for maintenance
inspections.
Inspector:
___________________________________________
Date of Inspection: ___________________________________________
Location of Records: ___________________________________________
Equipment
Satisfactory
Unsatisfactory
Comments/Action
University of Michigan - Dearborn
Environment, Health & Safety (EHS)
LABORATORY EQUIPMENT DECONTAMINATION
FORM
EQUIPMENT OWNER
Principal Investigator: ____________________________________________
Department: ____________________________________________________
Contact Phone Number: ___________________________________________
Building: _______________________________ Room Number: _________
EQUIPMENT INFORMATION
Equipment Type/Model: __________________________________________
Serial Number: __________________________________________________
Service/Transportation/Disposal Provider: ____________________________
Service to be performed: __________________________________________
RADIOACTIVE MATERIALS: If radioactive materials were used or
stored in the equipment, contact OSEH Radiation Safety Services (RSS) at
734-764-6200 to conduct a survey.
Did the RSS survey indicate detectable levels of radioactive contamination?
Check one Yes No
 N/A
CERTIFICATION OF DECONTAMINATION: I certify that the
above referencedlaboratory equipment has been thoroughly cleaned and
decontaminated of all chemical,biological, and radioactive contaminants.
_____________________
(Name Print)
________________________ ____________
(Signature)
(Date)
Complete and attach this form to equipment used for hazardous materials that is sent
outside the laboratory. Contact EHS at 313-593-4914 for questions about
decontamination or completing the form.
SAMPLE A
LABORATORY STANDARD OPERATING PROCEDURE
(for the use of hazardous materials or equipment)
Name of Procedure: Use of Benzene for Gel Electrophoresis
Prepared By: <Preparer’s Name>
Revision Date: 05/01
LOCATION – This procedure may be performed at the following location(s):
All procedures with benzene will be conducted in a chemical fume hood located in room
1234 ABC. Keep in tightly closed container. Store in rated flammable liquid storage
cabinet. Store with compatible materials, e.g., away from oxidizers.
USAGE – The following is a brief summary of how this material will be used.
One (1) liter container of benzene will be ordered. Each use will need 400 ml; two runs/per
experiment. Only one person will perform and use this gel system. There will be no more
than 3 runs (6 gels) performed between now and July 15, 2001. The employee has previous
training and experience with this protocol in Brazil. No other employees will be performing
this procedure after July 2001. See Protocol for additional details.
HAZARDS – The materials and equipment associated with this procedure present the
following exposure or physical health hazards. Safety precautions are prudent and
mandatory:
Carcinogen: Chronic exposure by inhalation may result in various blood disorders (anemia,
leukemia). Symptoms of acute exposure can lead to headaches, dizziness, nausea or
intoxications. May irritate eyes, nose and respiratory tract. Benzene can be absorbed
through the skin and may cause dermatitis.
Flammable
ENGINEERING CONTROLS – Prior to performing this procedure, the following safety
equipment must be accessible and ready for use, e.g., chemical fume hood, biological safety
cabinet, laminar flow hood, chemical spill kits.
This procedure must be conducted in a certified chemical fume hood located in room 1234
ABC. (Fume Hood Certification date 01/25/01)
PROTECTIVE EQUIPMENT – Prior to performing this procedure, the following
personal protective equipment must be obtained and ready for use:
Employee should double glove for prior to working with benzene. Disposable nitrile gloves
provide minimum protection for general laboratory use and should be changed frequently or
whenever contamination is suspected. Viton® gloves are required when hand immersion in
benzene is expected. Safety goggles should be worn when a splash hazard exists; safety
glasses with side shields are required at a minimum when benzene is used in a closed
system. A laboratory coat should be worn when working with chemicals. A chemically
resistant apron should be used if transferring or using large quantities of benzene in open
containers
WASTE DISPOSAL – This procedure will result in the following regulated waste that
must be disposed of in compliance with environmental regulations:
Benzene waste will be collected by manual aspiration of material. It will be placed in a
glass bottle, which will have a hazardous waste label and radioactive label. This waste
must be discarded within 90-days. The following information must be filled out on the
label before hazardous waste can be placed into the bottle: identification of contents
(Benzene), accumulation start date (the date material is first placed into the bottle as
waste), as well as the name of a laboratory researcher (Jim Smith), room number and
building (123 ABC). EHS (313-593-4314) will be contacted to dispose of the material.
ACCIDENTAL SPILL – In the event that a hazardous material spills during this
procedure, be prepared to execute the following emergency procedure:
If a small spill: Absorb with compatible material such as vermiculite or spill pads and
transfer absorbed material to a closed container. Label and date as hazardous waste.
Contact EHS for pickup (313-593-4914)
If a large spill: Notify others in room of spill. Extinguish all ignition sources; if safe to
do so. Evacuate room/immediate area. Call EHS (313-593-4914) or DPS (9-1-1 from
at campus phone or 313-593-5333) for cleanup. Post room with warning notifying
others of spill. Prevent unnecessary entry into area until arrival of EHS. Provide
assistance and information to spill responders.
In the event of a splash to the eyes or skin, immediately flush with water for 15minutes and seek medical attention at Midwest Health System.
PRIOR APPROVAL – This procedure is considered hazardous enough to warrant prior
approval from the Principal Investigator.
YES
NO
Principal Investigator’s
Signature:____________________________________________________________
ADDITIONAL PRECAUTIONS & REFERENCES:


Keep away from ignition sources. Incompatible with strong oxidizers or strong acids.
Wash hands thoroughly after use. Do not eat, drink or smoke in areas where benzene
or other chemicals are used.
LABORATORY STANDARD OPERATING PROCEDURE
(for the use of hazardous materials or equipment)
Name of Procedure: Use of Methylene Chloride
Prepared By: < Preparer’s Name >
Revision Date: 05/02/04
LOCATION – This procedure may be performed at the following location(s):
All procedures with methylene chloride (MeCl2) will be conducted in a chemical fume hood
located in lab at 321. Keep in tightly closed container. Store in rated flammable liquid
storage cabinet. Store with compatible materials, e.g., away from oxidizers, plastics, rubber,
nitric acid and chemically active metals.
USAGE – The following is a brief summary of how this material will be used.
Methylene Chloride is used as an extraction solvent. It removes Semi-Volatile Organic
Compounds (SVOC), Pesticides, and PCBs from both water and soil samples. Typically 50
ml are used per sample. The only difference is SVOC waters. To do those, add 60 ml
MeCl2, shake, drain the MeCl2, and repeat five more times. Total MeCl2 300 ml per sample.
All methylene chloride work is done inside a fume hood. The soil extraction unit is outside
the hood, but it is a self-contained system. See specific protocols for additional details.
HAZARDS – The materials and equipment associated with this procedure present the
following exposure or physical health hazards. Safety precautions are prudent and
mandatory:
Suspect Carcinogen (of bile duct and brain). Chronic exposure can cause headache, mental
confusion, depression, liver and kidney effects, and bronchitis. Symptoms of acute
exposure can lead to mental confusion, headaches, dizziness, nausea or intoxications. May
irritate eyes, nose and respiratory tract. Methylene Chloride can be absorbed through the
skin and may cause dermatitis or chemical burns.
Flammable.
ENGINEERING CONTROLS – Prior to performing this procedure, the following safety
equipment must be accessible and ready for use, e.g., chemical fume hood, biological safety
cabinet, laminar flow hood, chemical spill kits.
This procedure must be conducted in a certified chemical fume hood located in lab.
PROTECTIVE EQUIPMENT – Prior to performing this procedure, the following
personal protective equipment must be obtained and ready for use:
Employee should double glove prior to working with methylene chloride. Disposable
neoprene gloves provide minimum protection for general laboratory use and should be
changed frequently or whenever contamination is suspected. Safety goggles should be worn
when a splash hazard exists; safety glasses with side shields are required at a minimum
when methylene chloride is used in a closed system. A laboratory coat should be worn when
working with chemicals. A chemically resistant apron should be used if transferring or using
large quantities of methylene chloride in open containers
WASTE DISPOSAL – This procedure will result in the follow regulated waste which must
be disposed of in compliance with environmental regulations:
Methylene chloride waste will be collected by manual aspiration of material. It will be
placed in a glass bottle, which will have a hazardous waste label. This waste must be
discarded within 90-days. The following information must be filled out on the label
before hazardous waste can be placed into the bottle: identification of contents
(Methylene Chloride), accumulation start date (Date material is collected in bottle as
waste), as well as the name of a laboratory researcher, room number and building.
Contact EHS (313-593-4914) to dispose of the material.
ACCIDENTAL SPILL – In the event that a hazardous material spills during this
procedure, be prepared to execute the following emergency procedure:
If a small spill: Absorb with compatible material such as vermiculite or spill pads and
transfer absorbed material to a closed container. Label and date as hazardous waste.
Contact EHS for pickup (313-593-4914).
If a large spill: Notify others in room of spill. Extinguish all ignition sources.
Evacuate room/immediate area. Call EHS (313-593-4914) or DPS (9-1-1 from a
campus phone or 313-593-5333) for cleanup. Post room with warning notifying others
of spill. Prevent unnecessary entry into area until arrival of EHS. Provide assistance
and information to spill responders.
In the event of a splash to the eyes or skin, immediately flush with water for 15minutes and seek medical attention at Midwest Health Services.
PRIOR APPROVAL – This procedure is considered hazardous enough to warrant prior
approval from the Principal Investigator.
YES
NO
Principal Investigator’s
Signature:____________________________________________________________
ADDITIONAL PRECAUTIONS & REFERENCES:
*
Keep away from ignition sources. Incompatible with strong oxidizers and
metals.
*
Wash hands thoroughly after use. Do not eat, drink or smoke in areas where
methylene chloride or other chemicals are used.
Chemical Hygiene Plan: Standard Operating Procedure Examples
(Must be customized for each particular laboratory.)
Written safety, handling and waste disposal procedures are required for all hazardous
chemicals used in the laboratory. This includes all chemicals that are toxic, carcinogens,
reproductive toxins, irritants, corrosives, sensitizers, hepatotoxins, nephrotoxins,
neurotoxins, agents that act on the hematopoietic systems, and agents that damage the lungs,
skin, eyes, or mucous membranes. These procedures must be designed to adequately protect
the safety and health of workers in the laboratory and specify specific control measures
including: Engineering Controls, e.g., Fume Hood, Personal Protective Equipment, e.g.,
acid-resistant gloves, and Administrative Controls, e.g., limit duration of exposure or reduce
quantities involved in the process.
Most chemicals that are found in the laboratory can be grouped into categories, e.g., acids,
flammable liquids, etc., that have the same safety, handling and waste disposal procedures.
Chemicals fitting into these categories can be covered by general written procedures
designed for that category. Chemicals that present a particular hazard that is not adequately
covered in the general health and safety procedures must have specific Standard Operating
Procedures (SOP) that protect the worker. These procedures must consider how the
chemical is being used and all dangers involved with its use. Beyond writing the SOP, the
workers must also be trained in the use of these procedures. This includes the proper use of
safety equipment and engineering controls. Following are examples of both general and
specific SOP.
General SOP: Acids (strong acids, may cause skin and eye burns)
Skin and eye contact should be avoided at all times with the use of personal protective
equipment. All persons in the lab should wear safety glasses. Face shields, goggles and
shields should be used to provide better protection when pouring or manipulating large
volumes. Protection from contact with the skin is achieved through the use of gloves,
laboratory coats, tongs, and other protective devices. Gloves should be chosen with a high
resistance to acids, like Nitrile NBR gloves. The Nitrile NBR gloves and safety goggles are
stored in drawer XYZ. Always add acids to water when mixing.
Spills must be cleaned up immediately with the acid spill kit located in cabinet XYZ in
room ABC. Most acids are very slippery and may create a slip and fall hazard when spilled
on the floor.
In the event of skin or eye contact, the affected area should be rinsed with water for at least
15-minutes. Medical attention should be sought as soon as possible. Notify the lab
supervisor and report to Midwest Health Services.
Acids must be stored in the corrosive-resistant cabinet, with other compatible chemicals,
marked “acid” under the fume hood. Acids should never be stored with flammables or
organics. Do not store above eye level or out of view.
Manifest, label and dispose of acid waste according to EHS guideline. Call EHS at 313593-4914 for pick up of hazardous waste.
Specific SOP:
The SOP can be written for individual chemicals as shown below or as a preamble to your
experimental protocols. A “safety precautions” section to your current protocols should
include the same information including engineering controls, PPE and the hazards involved.
Use whichever method is easier for you; both are in compliance with the Laboratory Safety
Standard. If the SOP instructions are only included in your protocols please make reference
as to their location in the Chemical Hygiene Plan (CHP).
These examples are based on a general lab use of the chemicals listed. Procedures in your
lab may require extra precautions based on how the chemical is used, e.g., hot or pressurized
processes, unusually large quantities, mixtures or reactions with other chemicals, etc..
Acrylamide: neurotoxin, suspected human carcinogen.
Hazards: Highly toxic and irritant (skin, eyes, respiratory tract), causes CNS paralysis.
Routes: Toxic by inhalation and skin contact. Absorbed through unbroken skin.
Protection: Use premixed gel whenever possible. Weigh out dry material in a fume hood,
wear latex gloves, safety glasses and lab coat when handling, thoroughly wash hands when
finished. Take care not to create dust.
Chloroform: chlorinated solvent, suspected human carcinogen.
Hazards: Very volatile. Causes CNS depression and dermatitis, forms hazardous gases
when mixed with acids.
Routes: Toxic by inhalation and skin contact.
Protection: Always use in fume hood, wear Polyvinyl Alcohol (PVA) gloves, safety glasses
and lab coat when handling. Do not mix or store with acids, may form toxic gas.
Thoroughly wash hands when finished handling.
Diethyl Pyrocarbonate:
Hazards: Toxic and irritant (skin, eyes, respiratory tract), causes CNS paralysis. Refrigerate,
moisture sensitive, may build pressure in storage. Do not use past expiration date, date upon
opening.
Routes: Toxic by inhalation and skin contact.
Protection: Wear rubber gloves, safety glasses and lab coat when handling, open carefully
in fume hood. Thoroughly wash hands when finished.
Dimethyl Dichlorosilane:
Hazards: Highly toxic, flammable. Causes burns, reacts violently with water.
Routes: Toxic by inhalation and skin contact.
Protection: Use in fume hood, wear heavy rubber gloves, safety glasses and lab coat when
handling. Keep away from water and ignition sources. Thoroughly wash hands when
finished handling.
Ether:
Hazards: Causes narcosis and irritation (skin, eyes, respiratory tract). Very volatile and
extremely flammable; do not use near ignition sources. Forms explosive peroxides. Heat
and light speed peroxide formation. Can form peroxides in less than 12-months.
Routes: Toxic by inhalation.
Protection: Use in fume hood, wear Neoprene gloves, safety glasses and lab coat when
handling. Do not use or store near an ignition source, heat or oxidizers. Date upon opening,
do not use past expiration date. Do not distill to dryness. Thoroughly wash hands when
finished handling.
Ethidium Bromide: Toxic, mutagen.
Hazards: Irritating to eyes, respiratory system and skin. May cause heritable genetic
damage.
Routes: Toxic by inhalation, ingestion, and skin contact.
Protection: Use in fume hood, wear latex gloves, safety glasses, and lab coat. Thoroughly
wash hands when finished.
Formaldehyde: Suspected human carcinogen.
Hazards: Combustible. Highly irritating to mucous membranes. Carcinogen.
Routes: Toxic by inhalation and readily absorbed through skin.
Protection: Use in a fume hood, wear Nitrile gloves, safety glasses and lab coat when
handling. Thoroughly wash hands when finished.
Halogenated Anesthetic Gases
Hazards: Highly volatile, CNS depressant.
Routes: Toxic by inhalation.
Protection: firmly attach anesthetic delivery system to subject. Use in a well ventilated area,
e.g., fume hood, canopy hood. Use scavenger exhaust system and make sure tracheal
bubble is inflated during use.
Nitric Acid (70%): Strong acid.
Hazards: Strong oxidizer, causes burns, ignites combustibles, and reacts violently with water
and organics.
Routes: Highly toxic by inhalation and contact with skin or eyes.
Protection: Use in fume hood, wear Neoprene gloves, safety goggles, and a protective apron
when handling, thoroughly wash hands when finished. Do not store with flammables or
combustibles.
Osmium Tetroxide: Toxic.
Hazards: Highly toxic, vapor poisonous, irritant (skin, eyes, respiratory tract), causes
damage to eyes respiratory tract and skin, keep away from combustible materials.
Routes: Toxic by inhalation and skin contact.
Protection: Always use in a fume hood, wear latex gloves, safety glasses and lab coat when
handling, thoroughly wash hands when finished. Take care not to create dust.
Tetrahydrofuran:
Hazards: Volatile, flammable do not use near ignition sources. Forms explosive peroxides.
Date upon opening, and do not use past expiration date. Do not distill to dryness. Irritating
to eyes, respiratory system and skin.
Routes: Inhalation
Protection: Test for peroxide formation periodically and before distillation. Use and distill
only in a fume hood. Wear Viton gloves, safety glasses and lab coat when handling,
thoroughly wash hands when finished. Check that water is running during distillation and
do not let process run over night.
Phenol:
Hazards: Corrosive, highly toxic and combustible. Readily absorbed through the skin.
Ingestion of even small amounts may cause nausea, vomiting, circulatory collapse,
convulsions, coma, death, etc.
Routes: Highly toxic through inhalation and ingestion and readily absorbed through the skin.
Protection: Always use in a fume hood, wear Butyl rubber gloves, lab coat and safety
glasses. When working with large concentrated volumes wear goggles and a protective
apron. Thoroughly wash hands when finished.