Microscope Basics

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Mind Stretcher - Monday
Use two mind stretcher boxes for this!
Box 1: Tell me one experience you have
had with a microscope.
Box 2: List three things you know about cells. (Can
be anything……)
Spooky
Microscope
Trivia
Spooky Cells 1
My composition is
white and hard,
you can find me in
an old graveyard.
Focuses
Body ocular
Tube
lens
Rotates Objective Lenses for
Nosepiece
Viewing
Where
Ocular
you
lens
View
(Eyepiece)
the
Specimen!
Arm
EachObjectives
has Different
Magnification!
Stage
HoldStage
SlideClips
in Place
Regulates
Diaphragm
Amount of
Light going through
Light
Slide!
Coarse
Use forAdjustment
Broad
Scanning on Low
Use
forAdjustment
Fine
Power
Objectives!
Fine
Adjustments in High
Power Objectives!
Base
Spooky Cells 2
Some are smooth
and others are hairy,
Frankenstein's was
green and scary!
Microscope Lenses
• Eyepiece
– Holds Ocular Lens
– Magnification of 10X
• Scanning Objective Lens
– Magnification of 4x
– Used for Scanning
– Use Coarse Focuse Knob
• Low Power Objective Lens
– Magnification of 10X
– Used for Scanning
– Use Coarse Focus Knob
• High Power Objective Lens
– Magnification of 40X
– Used for Fine Focusing
• Seeing the details
– Use Fine Focus Knob ONLY
• Do NOT use Coarse Focus
Knob
The Light Source sends light through the slide.
The Iris Diaphragm regulates the amount of light from the
light source that passes through the slide.
The coarse adjustment should only be used in scanning and
low power objectives. It is used for seeing the slide.
You should use the fine adjustment while in the high power
objective. It is used for detailed focus.
The stage clips hold the slide and are located on
the stage which is moved to view different areas
of the slide and specimen.
All of the objectives are held on the nosepiece
which rotates to change the power of
magnification.
The scanning lens is usually 4x magnification and
is used for initial scanning and specimen
location on the slide.
The low or medium power objective lens is
usually 10x magnification and is also used
for scanning.
The high power lens is usually 40x or 100x
and is used for detailed viewing of a
specimen.
Spooky Cell 3
I can make some people
scream and squeal,
But to Edward Cullen I am
a favorite meal.
What’s my power?
To calculate the power of magnification, MULTIPLY the POWER
of the Ocular Lens by the POWER of the Objective
What is the power of
magnification for the Ocular
Lens (shown) and the 40x
Objective Lens (shown)?
Fill Your Calculations AND
Your answer on Your
Worksheet!!!
Comparing Powers of Magnification
We can see better details with higher the
powers of magnification, but we cannot
see as much of the image.
Which of these images
would be viewed at a
higher power of
magnification?
Do’s and Don’ts of Microscope
Labs!
• USE TWO HANDS
– Always carry microscopes with one hand on the ARM
and the other UNDER the BASE!
Do’s and Don’ts
• NEVER use Coarse
Focus Knob with
High Power Lens…it
Breaks Slides!
• “Ooops, I broke a
slide”
– Accidents Happen.
Don’t FREAK OUT!
– Don’t try to clean it up
by yourself!
– Raise your hand and a
teacher will help you!
Cleaning UP!
•
Always do 5 Things!
1.
2.
3.
4.
5.
Remove Your Slide
Wrap Your Cord
Lower Your Stage
Replace Plastic Cover
Put Microscope Back in
Designated Area Arm Out
Spooky Cell 4
Stretch me and work me
to build more bulk.
Soon you will look like the
incredible hulk!
Wrap Up Activity!
Wrap Up Quiz Directions:
1.
Log on to a computer
2.
Take out a Blank Sheet of Paper
–
–
–
3.
Write your name, date, and class in the top write hand corner.
Label the paper 1-14 without skipping lines
Flip the paper over and label 1-10 on the back
Go to:
–
–
Go to Class Blog and click on link.
Bottom Right hand Corner:
•
4.
Microscope Parts Quiz
Take the Microscope Parts Quiz Number One!
–
–
5.
As you answer the questions. Write your answers on your piece of paper.
When you have completed the quiz, write your given score at the top of your
paper.
When you have completed the quiz, go back, and take Microscope Parts
Quiz Number Two
–
–
Fill in your letter answers on the BACK of your sheet of paper.
Write your score above your answers!
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use
too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover
Slip
Lower slowly
5 – Place the slide on the stage and view it first with the red-banded
objective. Once you see the image, you can rotate the nosepiece to
view the slide with the different objectives.
You do not need to use the stage clips
when viewing wet-mount slides!
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