Unit 1 - Cells: The Functional Unit of Life

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Unit 4 –

Photosynthesis and

Cellular Respiration

Reading quiz (mitochondrion and chloroplast structure)

Draw a LARGE, DETAILED, labeled sketch of a chloroplast

Draw a LARGE, DETAILED, labeled sketch of a mitochondrion

1.25 point for having a drawing “somewhat similar to below”.

½ a point for each term labeled: . Chloroplast: Inner membrane outermembrane thylakoid

Mitchondrion: outermembrane innermembrane

Add any term not labeled to your diagram

Title each of the following pages

 Photosynthesis overview

 Plant pigments

 Reading quiz/Light reactions

 Calvin Cycle

 Photosynthesis lab

 Reading quiz/Cellular respiration overview

 Glycolysis and Krebs Cycle

 Electron Transport Chain and Fermentation

 Comparing Photosynthesis and Cellular Respiration

Photosynthesis overview

Process of storing energy in sugar molecules from the energy initially in the sun (radiant energy)

1 st step: Capture radiant energy and use it to generate our “energy currency”

2nd step: Use “energy currency” to convert

CO

2 to glucose

Oxygen is released as a byproduct

Happens in the chloroplast

Plant pigments

Problem: How do plants utilize radiant energy to produce sugars?

Properties of light

While traveling, acts as a wave (properties depend on this wavelength)

When interacting with matter (like your clothes) acts as a particle

Photon: Discrete packet of light

Plant pigments

 Pigments are materials that absorb particular wavelengths of light and reflect others

 Why do your eyes have a ___ color?

 When chlorophyll absorbs light, energy is transferred to electrons and “boosts” them to a

Plant pigments higher state.

Reading quiz

 Where do the light reactions occur?

 Where does the Calvin cycle occur?

 What is the purpose of the light reactions?

 What is the purpose of the Calvin cycle?

Light reactions

What you need to know:

Location –

_________

Input – ___________

Output – _______

Purpose - ________

Light reactions

Photosystem

 Role of chlorophyll:

Capture energy from light

 Role of an electron carrier: transport electrons

Tracking electrons and energy

NADP + + 2e + H +  NADPH

Using Elodea to demonstrate

Photosynthesis

6CO

2

+ 6H

2

O light

>

C

6

H

12

O

6

+ 6O

2

How can we determine if photosynthesis occurs?

Measure ______

How?

CO

2 can turn into carbonic acid

Bromothymol blue will be blue at pH around 7.6 and yellow-green at pH of 6

Some CO

CO

2

2 will turn into Carbonic acid (so if enough is present in a bromothymol blue solution, it will have a ___color.)

If a initial color of yellow-green turns blue then what can you infer?

Lab setup

b.b. + Elodea + light b. b. + Elodea + dark b.b. + light

 As a pair, make a hypothesis. The presence of light (will/will not) affect the rate of photsynthesis and (will change the color of the solution to blue/will not change the color of the solution)

1. Obtain tray

2. Place a 3 cm piece of elodea in 2 vials

3. Fill each of the 3 vials with bromothymol blue

4. Cap each vial and cover 1 of the elodea vials with the aluminum foil

5. Keep vials in test tube rack and place back EXACTLY where it came from. THEN…

6. Work with your partner. On the paper provided, diagram the entire light reaction from memory first. Once you did as much as you can, use your notes and study guide (no textbook). Practice explaining the entire process, including what happens to water, chlorophyll a, electrons, electron transport chain, hydrogen ions, and how ATP,

NADPH are produced

7. When you can successfully explain all this, call me and I will initial your assignment sheet when you can do it perfectly. If you get initialed, you might be asked to go around checking others.

Calvin Cycle

 Location-

Purpose-

Input –

Output –

 Tracing carbon

 Factors affecting photosynthesis

Reading quiz

 What organisms does cellular respiration occur in?

 Write the cellular respiration equation

 What are the inputs of glycolysis?

 Does aerobic respiration refer to having oxygen or lacking oxygen?

Cellular Respiration – Overview

(SG 4A, 4B, 4C)

Process of forming ATP by breaking down glucose in the presence of oxygen

6O

2

+ C

6

H

12

O

6

 6CO

2

+6H

2

O + energy

Why do we breathe (external respiration)?

Why do we consume and digest carbohydrates?

Does this happen in one step?

Glycolysis

 Series of chemical reactions that break down one molecule of glucose into pyruvate

C

6

H

12

O

6

 2C

3

H

6

O

3

 Happens in cytosol of the cell

Inputs

Glucose

2 NAD+

2 ADP + 2 P

2 ATP

Outputs

2 pyruvate

2 NADH

4 ATP (net 2)

Draw this chart (under cell respiration overview) and fill in the following terms glycolysis, ATP, NADH, glucose, pyruvate

Photosynthesis lab continued (write analysis on a blank paper)

 Fill out the following chart b.b. + light b.b. + elodea b.b. + elodea

(dark) + light

0

60 min.

What color did the elodea + b.b. + light change to?

What pH will bromothymol blue be green? When will it be blue?

So, the color changed DIRECTLY due to a change in ___

The change in ___ was caused by a change in ____

The change in ___ was caused by a change in ___

The change in ___ was caused by a change in what process ___

How can we get the b.b. in front of you back to green?

Use all of this information to write a well coherent allows to support or not support the following hypothesis: Light will increase the rate of photosynthesis.

My hypothesis was/was not supported. (restate hypothesis). Discuss color change in all 3 tubes. Discuss what chemical changes caused the color change. Speculate why light increased the rate of photosynthesis.

Citric acid (Kreb) cycle

 Series of reactions that finishes glucose break down to carbon dioxide in the presence of oxygen (aerobic)

 Happens in matrix of mitochondria

Inputs

2 pyruvate

2 ADP + 2 P

6 NAD +

2 FAD

Outputs

4 CO

2

2 ATP

6 NADH

2 FADH

2

Cellular Respiration – Electron

Transport Chain

Process that uses high energy electrons from Krebs cycle to convert ADP to ATP

Location: cristae of mitochondria

32 total ATP

Fill out the following chart to compare

Photosynthesis and Cellular Respiration

Photosynthesis Cellular Respiration

Function

Location

Reactants

Products

Equation

Photosynthesis – Cellular

Respiration comparison

Photosynthesis

Function Energy capture

Location Chloroplasts

Reactants CO

2 and H

2

O

Products C

6

H

12

O

6 and O

2

Equation 6CO

2

+ 6H

2

O light

>

C

6

H

12

O

6

+ 6O

2

Cellular Respiration

Energy release

Mitochondria

C

6

H

12

O

6 and O

2

CO

2 and H

2

O

6O

2

+ C

6

H

12

O

6

6CO

2

+6H

2

O + energy

Fermentation (anaerobic respiration)

 Process that releases some energy from glucose when no oxygen is present

Plants, yeast and few microbes Other organisms

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