LAB #11:
MAMMALIAN ANATOMY
Histology, Fetal Pig Dissection
Fall 2005
Histology
The slides you will be examining
Photos by T. Chubb/J. Trout
Epithelial Tissue
Kidney
(Simple Cuboidal Epithelium)
40 x
100x
400x
Kidney
(Simple Cuboidal Epithelium)
40 X
100 X
This is from a slightly
different area of the
kidney
400 X
Esophagus
Stratified Squamous Epithelium
(non-keratinized)
40 X
100 X
400 X
Connective Tissue
Bone
Central canal
100 X
osteocyte
All the little fine
black lines are the
caniliculi
400 X
Blood
http://www.gihealth.com/images/imgIronDeficiency2.jpg
Muscle Tissue
Skeletal Muscle
Cross section do not use
40 X (ls)
40 X
nucleus
100 X (ls)
400 X (ls)
Cardiac Muscle
40 x
100 x
nucleus
400 x
Smooth Muscle
100 x
40 x
400 x
400 x
(cs)
(ls)
Nervous Tissue
Nervous Tissue
Cell body of neuron
100x
400x
Fetal Pig Dissection
Dissection:
Definition: The process of careful
separation for detailed
examination & analysis.
• TAKE YOUR TIME!
• Look BEFORE you cut!
Fetal Pig
After external examination,
make your initial cuts as
shown
(follow your lab manual).
http://www.ekcsk12.org/science/lelab/incisionsfetalpig.gif
Internal Anatomy
Following the lab manual,
carefully dissect your
specimen.
Find all features
listed on your
“Checklist of Structures”.
http://home.satx.rr.com/christenson/Churchill%20Biology/dissec13.jpg
Fetal Heart vs. Adult Heart
Is there a difference?
Why is there a difference?
….Think about it!
NORMAL HEART (Adult)
NORMAL HEART
www.mirage-samoyeds.com/ heart.htm
PATENT DUCTUS ARTERIOSIS
www.mirage-samoyeds.com/ heart.htm
DEMO: Sheep Heart Dissection
External
Examination
Use the
demonstration
sheep heart to
complete your
“Heart Diagram”
Handout.
http://science.tjc.edu/images/preserved_heart/cut_heart_anterior_label.jpg
DEMO: Sheep Heart Dissection,
(cross section view)
http://www.sunyniagara.cc.ny.us/val/sheep-heart-3.jpg
DEMO: Sheep Brain
(lateral view)
http://www.sunyniagara.cc.ny.us/val/sheep-brain-lateral.jpg
DEMO: Sheep Brain
(inferior view)
TODAY’S PLAN:
1. Observe histology slides (8). Sketch & label on
your “Tissue Types Handout”.
(use your lab manual as a guide, Ex. 32).
2. Fetal Pig Dissection: done in pairs. Follow
handout & use your manual, Ex. 33, 34*, 35.
Identify all structures on the “Checklist of
Structures for the Fetal Pig Dissection”.
3. Observe DEMOs: Sheep Heart, Brain, Shark.
Post-Lab Assignment:
To be completed TODAY: (1) histology slide
sketches, (2) I.D. pig anatomy, (3) adult
heart diagram.
To be turned in NEXT WEEK: Lab Manual
Questions Exercise 34, pages 573-574.
CLEAN UP!
• Dispose of all specimens in the RED
BIOHAZARD BAG/BOX provided.
• Wash all dissection tools & set out to dry.
• Wipe down your lab bench.
• Wash your hands before leaving.