Lab Exercises 1. Road crossing time for all animals [Google Earth] 2. Possum Start/end activity from ACC 3. Male Raccoon Start/end activity from ACC 4. Female Raccoon Start/end activity from ACC 5. ODBA Over time [excel & pivot] 6. Distance moved per night, over time [excel & pivot] 7. Interactions between individuals. 8. Plot den sites (daytime locations) 1. Road Crossings • Question: What time of day do animals cross the roads? • Methods: – Download google earth data in 1-week intervals starting 8/26/13 for each animal . – Step through animals nightly tracks slowly – Identify crossings, note ‘local time stamp’, estimate time f crossing – Record data in excel sheet • Results: – Create Google map with color coded dots showing interesting patters (species/time etc…) – Make a graph showing your results 2, 3, 4, Start and End of Activity • Question: what time of day do the animals start and end their activity period? • Methods: – Download ACC Viewer, be sure to update Java, run program. https://www.3dyne.com/movebank-acceleration-viewer/download/ – Within Program, Download data from tag id: – Login – Zoom in/out, note start/end of activity for each night of activity (float cursor over cell and note date_time in bottom left), record in excel. Note time is UTC, but excel is set up to convert to EST – Type in sunset/sunrise times for each date (suggest on separate laptop) note daylight savings time (+1hr) and get from http://aa.usno.navy.mil/data/docs/RS_OneYear.php – Note any extensive daytime activity • Results – Graph Start time and sunset over time – Graph end time and sunrise over time – Graph active time period length over time 5. Energy Expenditure over time • Question: how does the energy expenditure/activity level vary over time? • Methods – Download ACC Viewer, be sure to update Java, run program. https://www.3dyne.com/movebank-acceleration-viewer/download/ – Within Program, Download data from tag id: – Login – Experimental/export ODBA, save file, open in excel – Make day, hour, and day_hr column in excel (cut_paste excel blue text) – Use excel Crosstabs to calculate average values per day, hour, and day_hr. cut/paste these values into a new worksheet. – Repeat for all 3 animals • Results – – – – Graph average daily odba over time. Graph average hourly odba over time Graph averge day_hr odba over time Bonus, plot hourly averages for each day as separate lines on the same graph 6. Distance moved per night • Question: How far did the animals move per night. • Methods – – – – Download data from Movebank as excel Delete all rows missing location data Sort by animal, then ‘local timestamp’ Use UTM coordinates & Pythagorean's theorem to calculate the distance moved in each time step. – Make day, hour, and day_hr column in excel (blue text in odba worksheet ) – Use excel Crosstabs to calculate average values per day, hour, and day_hr. cut/paste these values into a new worksheet. – Repeat for all 3 animals • Results – Graph average daily movement rate over time. – Graph average hourly movement rate over time – Graph averge day_hr movement rate over time 7. Interactions between animals • How often, and where, do animals interact • Methods – – – – – – Download from: http://web.engr.illinois.edu/~klei2/movemine/ Run the the file: MiningToolsFx.jar Login: Select NCSU Campus Carnivores, Download from server Set Gap(min) 15, ThreshGap(hr) 2 Click Mining/ Distance Calculation, save image (png) and data (csv). Look at data – Click mining/significance. Keep 100 rounds of simulation, try 20, 50, 100, 250m. Save the ones you think are meaningful. – Click mining/plot trajectories. Get KML file. Try each pair of individuals. Start with 25m and 30min, then try other variations. – Open KML’s in Google Earth, save interesting maps. • Results – Distance and significance matrices – Screen/shots telling main results of interaction sites 8. Den Sites • Question: where do animals spend the day? • Methods – – – – – Download data from Movebank as excel file Make a new column for hour using local time Load data into GIS as shape file Make map showing results Summarize results per animal (# of dens) and per den site (# of visit) • Results – Map(s) showing dens – Graphs showing summary statistics Movebank Download 1. Movebank.org Click the Map 2. Search NCSU Click magnifying glass to zoon Click + to open animals Check options 3. Click the i button ‘studies page’ Has dates, overview Go back to map 4. I button Download Select data type, download type, date range ACC How To • • • • • • Red/Blue/Green shows x/y/z axes accelerometer values Yellow bars at top show where GPS data is collected Mouse wheel to zoom in/out Click/Drag to move right/left Zoom in to see gray bars showing separate bursts Float cursor over data to see date/time in bottom left, in UTC Google Earth How To Use +/- to zoom in/out on time scale Move front & rear slider to control how many dots are shown at once Use forward & reverse arrows to move forward/backward in time steps (step length will depend on zoom level) These are our results from 2013 Hector’s Homerange Heidi Homerange Hilda Homerange Daily ODBA 16000 14000 12000 ODBA 10000 Hector 8000 Heidi Hilda 6000 4000 2000 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Day Daily energy use per day over the course of the study. Interesting to see the different overall values between the three animals, and also the few days adjustment at the beginning after the anesthesia (day 1) Accelerometer Data: Active vs. inactive periods Initial data – begins during anesthesia recovery Daily Activity patterns (from ODBA) Possum starts activity later and continues all night. Raccoon starts earlier but has midnight rest followed by early AM pulse (bimodal) 20000 20000 18000 18000 Possum Raccoon 16000 16000 14000 14000 ODBA 12000 12000 10000 10000 8000 8000 6000 6000 4000 4000 2000 2000 00 11 22 33 44 55 66 77 88 99 10 10 11 11 12 12 13 13 Hour 14 14 15 15 16 17 18 19 20 21 22 23 24 Opossum active period length over time Active Period 12:00:00 Number of Hours Active 9:00:00 6:00:00 3:00:00 0:00:00 1 6 11 16 21 26 31 Day Number 36 41 46 51 56 Male raccoon start and end of activity vs. sunset and sunrise 20:38 20:09 19:40 19:12 18:14 Start Time 17:45 Sunset 17:16 16:48 16:19 15:50 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 8 Day Day 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 0:00 1:12 2:24 3:36 Time Time 18:43 4:48 6:00 7:12 8:24 9:36 Sunrise End Time Female raccoon start and end of activity vs. sunrise and sunset Pushes activity much closer to dawn than the male 19:12 18:43 18:14 Time of Day 17:45 17:16 Start time 16:48 Sunset 16:19 15:50 15:21 14:52 9:36 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Study Day 8:24 Time of Day 7:12 6:00 4:48 End time 3:36 Sunrise 2:24 1:12 0:00 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Study Day Time of day they crossed roads No avoidance of busy periods by raccoon, but some by possum Male Raccoon Female Possum 4 14 12 3 Number of times Number of times 10 8 6 2 4 1 2 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Time (military time) 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Time (military time) Total Daily Movement Total Daily Movement of Study Animals 4000 3500 Distance Traveled (Meters) 3000 2500 Hector 2000 Heidi 1500 Hilda 1000 500 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Day of Study Night Hector got hit by a car Possible Meeting Locations of Hector (raccoon) and Hilda (Opossum) Actual Meeting Locations of Hector (Raccoon) and Hilda (Opossum) NONE Possible Meeting Locations for Hilda and Heidi Actual Meeting Locations for Hilda and Heidi NONE Heidi (Raccoon) and Hector (Raccoon) Actual Meeting Locations