Name ______________________________________ Group _______ Period ________ Ecology Lab: Intertidal Zonation Developed by Anne Maben, UCLA Science Coach for Fremont HS Introduction: Scientists have been aware of vertical zonation in intertidal habitats since the early 1800’s, long before “ecology” was even recognized as a branch of science. Early naturalists observed that the organisms found between the high and low tide marks seemed to vary in a consistent way. Where organisms are found in the intertidal depends upon their responses to exposure to air, heat, and sunlight, predation or grazing, and competition for space. Scientists have since discovered that intertidal habitats can be divided into four zones according to % coverage by the tides: the splash zone (never covered by water) and the high (covered ¼ of the day), middle (covered ½ of the day), and low (covered ¾ of the day) intertidal zones. For example, periwinkle seashells might be found in the upper splash zone, gooseneck barnacles and mussels in the middle intertidal, and sea hares and octopuses in the lowest levels. Wave action and substrate (sand, rock or mud) and chemical factors (pH, salinity, dissolved oxygen, nitrates, phosphates and pollutants) other also play important roles. We will try to document zonation by running a transect line from high to low levels in the local intertidal habitat, and comparing the species diversity (number of different species) and abundance (number of individuals from one species) of the various animals and plants along that transect. Our data will help us understand the biodiversity of our coastal marine life and how different abiotic factors (chemical and physical variables) may influence a species survival. Materials and Methods: 1) From your starting location place a 1x1 wooden square on a rock observe and record the organisms you see within the box. 2) Move your box approximately ten feet towards the ocean and repeat step one. Continue to this method until you have data for ten boxes. 3) Use field guides to select species that are easy to identify and fairly common or especially interesting to observe. Remember that many of these animals are small, often camouflaged, and may be hiding. Get down on your hands and knees for a closer look. Be sure to examine the undersides of rocks (please replace them in their original position), ledges, and even blades of algae. DO NOT TURN YOUR BACK TOWARDS THE OPEN OCEAN. 4) Record the data on your data sheet. Results and Analysis: Table 2. (Give it a proper title) # of Organisms per Species Name of Species QUAD 1 QUAD 2 QUAD 3 QUAD 4 QUAD 5 QUAD 6 QUAD 7 QUAD 8 QUAD 9 QUAD 10 1. 2. 3. 4. 5. 6. 7. 8. 9. 10 11. 12. Unidentified 3) Prepare graphs showing the relative abundance of each species in each quadrat. You will do this on your own at home. Ask your teacher for graph paper. DISCUSSION QUESTIONS: (to be answered on a separate paper, pen or typed, in detail.) 1. Discuss the general trends of biodiversity and species distribution of intertidal plants and animals in your study area. Did you find any species which appeared to cluster together in about the same places along the transect? Give examples of any species clusters you observed, and discuss why they may have occurred. 2. What ecological forces might be influencing the different distributions of these species? For example, what stresses could animals have if they lived higher in the intertidal? What stresses come with living lower in the intertidal? 3. If you could go back to the site next year, what additional data would you want to collect or what questions would you like to research about your study site?