Bio 211
A & P II
Lab Exercises
1
Biology 211 - A & P II
Laboratory Exercises
Required Materials and Safety Regulations for Bio 211 Labs:
Latex gloves: a box may be cheaper since you will need them ALL semester
Safety goggles or glasses: sunglasses are NOT acceptable
Colored pencils: the physical “act” of drawing the tissues is correlated with higher grades on the Bio 210 &
211 quizzes. In fact, the supplemental anatomy coloring book is an excellent means of learning the material;
the integration of vision and drawing reinforces the information.
Possibly closed-toed shoes: think of a dropping a scalpel and piercing your toe! For consistency and
safety, this may be required for every lab and quiz, even those in which a dissection is not scheduled.
Possibly a lab coat or lab apron: when purchasing, please keep in mind that you will also need a lab
coat for Bio 225 Lab – Microbiology lab
Exercise II-1
Endocrine System
Materials:
Lab 1:
Colored Pencils
Slides – specific slides listed on Objectives for Lab Quiz #1
Microscopes, transparencies, CD, and Web slides
Torso models and other models with endocrine glands
Lab 2:
Fetal Pig – container and solution for storage
Dissected cat
Dissection instruments and tray
References:
Atlas – refer to index for pages
Textbook
Instructions for Lab 1 & 2:
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During Lab 1, the lab instructor will review the location of the endocrine glands using models, Power
Point slides (PPT) or transparencies and use the teaching microscope or PPT slides to identify pertinent
regions, structures, features and hormones produced.
The student will use their microscope to identify the regions, features and structures of the glands/organs
as given on the Objectives for Lab Quiz #1 and sketch the tissue observed on the slides – space provided
on the following pages. The student will identify the hormone produced by each region of the gland/organ.
During Lab 2, the lab instructor will demonstrate & describe the procedure for opening the fetal pig before
the student begins dissecting. The student is responsible for identifying glands/organs and the
corresponding hormones on the fetal pig and the prosected cat as directed by the instructor.
The instructor will inform the student of the procedure for preserving the pig, as it will be used for the
remainder of the semester.
The student will select an endocrine topic for Exercise II-4.
Questions/Notes: Describe the locations & appearance of the endocrine glands on fetal pig and cat.
Bio 211
A & P II
Lab Exercises
2
Endocrine Glands and Hormones
ANTERIOR PITUITARY
1. _________________________
2. ____________________________________
3._______________________
4. ___________________________
5. ____________________________________
6. ______________________
POSTERIOR PITUITARY
(stored)
1. ______________________
2. ___________________aka______________________
THYROID
1. ______________________________
2. _______________________________
3. ______________________________
PARATHYROID
1. ______________________________
ADRENAL MEDULLA
1. ______________________________ 2. _____________________________
ADRENAL CORTEX
1-3) General class & a specific
1. _________________________________________________
Hormone
2. _________________________________________________
3. _________________________________________________
PANCREAS
1. ______________________
2. ______________________
THYMUS
1. ______________________
2. ______________________
PINEAL
1. ______________________
OVARIES
1. ______________________
TESTES
1. ______________________
2. ______________________
Know the name and abbreviation for each hormone.
Bio 211
A & P II
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
3
Bio 211
A & P II
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
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_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
4
Bio 211
A & P II
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
5
Bio 211
A & P II
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
6
Bio 211
A & P II
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
7
Bio 211
A & P II
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
8
Bio 211
A & P II
Lab Exercises
9
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Exercise II-2
Blood
Materials:
Slides – prepared blood slides & specific leukocyte slides
Microscopes
Blood Cell Poster
Additional Materials provided by student:
Colored pencils
References:
Atlas and textbook
Instructions:
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The lab instructor will review the types of blood cells and give their functions. The components of blood
will be discussed with detail as given on the Objectives for Lab Quiz #1.
The student will be able to identify the formed elements and specific leukocytes from blood slides and
will sketch them on the following pages.
The student will do a differential white blood cell count. Count approximately 50 leukocytes on the slide
and specify the type leukocyte. Use the chart below and calculate the percentage of each. Compare
this to the % expected from a normal blood sample (from text or instructor).
Questions/Notes:
Bio 211
Differential WBC:
Leukocyte
A & P II
# present
Sketch the formed elements in blood
Erythrocytes
Magnification: _____________
Description:_______________
_________________________
_________________________
Platelets
Magnification: _____________
Description:_______________
_________________________
_________________________
Leukocyte:______________________
Magnification: ______________
Description:________________
_________________________
_________________________
% of Total Counted
Lab Exercises
% Expected
10
Bio 211
A & P II
Leukocyte:_____________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Leukocyte:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Leukocyte:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Leukocyte:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
11
Bio 211
A & P II
Lab Exercises
12
Exercise II-3
Lymphatic System
Materials:
Slides – lymphatic vessel, lymph node, spleen, tonsils, ileum – Peyer’s Patchs
Microscopes
Torso model & head model & any models with lymph nodes
Additional Materials provided by student:
Colored pencils
References:
Atlas and textbook
Instructions:
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The lab instructor will review the location and functions of the lymphatic tissue referring to the
Objectives for Lab Quiz #1.
The student will locate the lymphatic tissue on models.
The instructor will explain how to distinguish the various lymphatic slides. The student will use the
microscope to find the lymphatic tissues listed in the objectives. Sketch these slides on the following
pages.
The student will be able to identify the locations of lymphatic tissue on the fetal pig and cat.
Questions/Notes:
Sketch the lymphatic tissues
Lymphatic Vessel
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Bio 211
A & P II
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Tissue:________________________
Magnification: _____________
Description:_______________
_________________________
_________________________
Lab Exercises
13
Bio 211
A & P II
Lab Exercises
14
Exercise II-4
Endocrine Dysfunction Oral Report
POSSIBLE ENDOCRINE DYSFUNCTION TOPICS
Addison’s Disease
Hyperthyroidism:Graves’ Disease
Hypothyroidism: Cretinism
Simple Goiter (Endemic Goiter)
Diabetes MellitusType I
Giantism
Dwarfism
Hormonal Infertility: Male
Adrenal virilism: Hirsutism
Hyperparathyriodism
Anabolic steroids - Medical usage
Growth hormone therapy
Testosterone Replacement therapy
Hyperprolactinemia/Dopamine Antagonists
GHB Use
Pheochromocytoma
Cushing’s Syndrome
Hyperthyroidism: Exophthalmic Goiter
Hypothyroidism: Myxedema
Diabetes Insipidus
Diabetes Mellitus Type II
Acromegaly
SAD – seasonal associated disorder (melatonin)
Hormonal Infertility: Female
Panhypopituitarism (Simmons’ Disease)
Hypoparathyroidism
Erythropoietin usage by athletes
Estrogen/Progesterone Replacement therapy
Multiple Endocrine Neoplasia (MEN) cancers Type I & II
Androgen Abuse/ Anabolic steroid usage by athletes
General Adaptation Syndrome (GAS)
If you know of and are interested in an Endocrine Disorder that is not listed and you would like to write your
report on it, please ask your Instructor; it may be welcome.
Each student will select an Endocrine Dysfunction or hormone disorder either from the list or an approved
topic. Sign up with your instructor. This oral report will count as 4 points on the first lab quiz.
Name of disorder:
Description of disorder and symptoms (relate to the Endocrine System):
Identify the cause:
Treatment: