SBI 4U 1 Name: _______________________ Knowledge Thinking/Investigation Cellular Respiration Quiz Part A: Knowledge – Multiple Choice (24 marks) _____ 1. Which of these processes occurs in the cytoplasm? a) Krebs cycle c) glycolysis b) electron transport chain d) pyruvate oxidation _____ 2. Complete oxidative breakdown of glucose results in ____ ATP molecules. a) 2 b) 4 c) 32 d) 36 e) 39 _____ 3. Pyruvate oxidation breaks a) glucose into pyruvates b) pyruvates into glucose c) pyruvates into acetyl-CoA and carbon dioxide d) pyruvates into acetyl-CoA and water e) acetyl-CoA into pyruvates and carbon dioxide _____ 4. The first reaction in the Krebs cycle combines a) carbon dioxide to a four carbon molecule b) carbon dioxide to a five carbon molecule c) acetyl-CoA to a four carbon molecule d) acetyl-CoA to a five carbon molecule _____ 5. Glycolysis yields about ___ of the energy in glucose in ATP molecules. a) 2% b) 15% c) 28% d) 39% _____ 6. Which process produces both NADH and FADH2? a) glycolysis b) the electron transport chain c) the Krebs cycle d) fermentation e) pyruvate oxidation _____ 7. Which process produces alcohol or lactate? a) glycolysis b) the electron transport system c) the Krebs cycle d) fermentation e) pyruvate oxidation /29 /8 _____ 8. Which process reduces molecular oxygen to water? a) glycolysis b) the electron transport chain c) the Krebs cycle d) fermentation e) pyruvate oxidation _____ 9. In which process does oxidative phosphorylation occur? a) glycolysis b) the electron transport chain c) the Krebs cycle d) fermentation e) pyruvate oxidation _____ 10. One turn of the Krebs cycle produces a) 2 NADH, 2 FADH2, 2 ATP b) 3 NADH, 1 FADH2, 1 ATP c) 1 NADH, 3 FADH2, 2 ATP d) 3 NADH, 2 FADH2, 1 ATP e) 3 NADH, 1 FADH2, 2 ATP _____ 11. Acetyl-CoA is produced from a) pyruvate and a coenzyme b) citric acid and a coenzyme c) ATP and pyruvate d) carbon dioxide and pyruvate e) citric acid and carbon dioxide _____ 12. The carbon dioxide (CO2) we exhale is produced in a) glycolysis c) the electron transport system b) lactate fermentation d) the Krebs cycle _____ 13. The primary energy carrier between the Krebs cycle and the electron transport system is a) NADH b) ADP c) FADH2 d) water (H2O) e) carbon dioxide (CO2) _____ 14. Which process must occur before fermentation? a) Krebs cycle b) glycolysis c) the electron transport chain d) pyruvate oxidation _____ 15. Which of these pairs of processes are anaerobic? a) fermentation and glycolysis b) fermentation and the Krebs cycle c) glycolysis and the Krebs cycle d) the Krebs cycle and the electron transport chain e) glycolysis and the electron transport chain _____ 16. Which of the following statements is not true? a) Obligate aerobes must live in oxygen presence b) Facultative anaerobes can live in the presence or absence of oxygen c) Obligate anaerobes must live in the absence of oxygen d) Chemoautotrophs were the last type of organism to establish themselves on Earth e) Heterotrophs rely on autotrophs for energy _____ 17. What is the value of the alcohol fermentation pathway? a) It produces ATP b) It produces lactate c) It produces ADP for the electron transport chain d) It replenishes pyruvate so that the Krebs cycle can occur e) It replenishes NAD+ so that glycolysis can produce ATP _____ 18. What is the purpose of oxygen (O2) in aerobic respiration? a) Oxygen accepts electrons at the end of the electron transport chain b) Oxygen is necessary to carry away the waste carbon dioxide c) Oxygen is used in the formation of sugar molecules d) The oxygen molecule becomes part of the ATP molecule e) Oxygen donates H+ used in the formation of NADH _____ 19. Substrate level phosphorylation takes place in a) glycolysis and the Krebs cycle b) the electron transport system and pyruvate oxidation c) glycolysis and the electron transport chain d) the Krebs cycle and pyruvate oxidation e) Both B and D are correct. _____ 20. At the end of glycolysis, each molecule of glucose has yielded 2 molecules of _____, 2 molecules of ____, and a net of 2 molecules of _____. a) lactic acid; NADH; ATP b) ethanol; NAD+; ATP c) pyruvate; NADH; ADP d) pyruvate; NAD+; ADP e) pyruvate; NADH; ATP _____ 21. The number of ATP made by each step of cellular respiration (including that contributed by the intermediate energy carriers) is as follows by the end of the whole process: a) Glycolysis: 6, Pyruvate Oxidation: 8, Krebs Cycle: 22 b) Glycolysis: 4, Pyruvate Oxidation: 6, Krebs Cycle: 26 c) Glycolysis: 6, Pyruvate Oxidation: 4, Krebs Cycle: 26 d) Glycolysis: 8, Pyruvate Oxidation: 6, Krebs Cycle: 22 e) Glycolysis: 6, Pyruvate Oxidation: 6, Krebs Cycle: 24 _____ 22. NADH generated by glycolysis produces less ATP molecules than that generated by the Krebs cycle because a) It enters the electron transport chain at a later point in the chain b) It is not the same type of NADH molecule c) Most of its energy is lost as heat d) The process by which ATP is made in glycolysis is different from that of the electron transport chain ____ 23. Proteins are to ribosomes as ATP is to which of the following cell organelles? a. centrioles d. chromosomes b. mitochondria e. nucleus c. Golgi apparatus ____ 24. When glucose undergoes combustion in the presence of air, it releases heat rapidly. In cellular respiration a. all of the energy is converted to ATP b. enzymes make the reaction take place in small steps c. water prevents uncontrolled combustion d. no oxygen is needed e. no heat is released Part B: Knowledge – True or False (5 marks) _____ 25. In the first half of glycolysis, glucose is activated by two molecules of ATP _____ 26. This reaction takes place in both animal cells and plant cells: C 6 H12O6 + 6O2 6CO2 + 6H2O _____ 27. NADH is reduced by the first protein complex of the electron transport chain _____ 28. In the Kreb’s cycle, acetyl-CoA combines with oxaloacetate to form isocitrate. _____ 29. ATP is formed when hydrogen ions move across the mitochondrial membrane to an area of greater hydrogen ion concentration Part C: Thinking/Investigation – Short Answer (8 marks) Answer the following question in the space provided. 30. Imagine that an extraterrestrial life-form has been recovered from a meteorite by a deep space probe that has a ravenous appetite for sand. Your job is to come up with a preliminary analysis of its bioenergetic pathway. On close examination you note that the biochemical pathway employed by this creature has some features in common with your own. You note that the alien has a basic cellular structure similar to that of Earth-based creatures. It has what you would consider to be a cytoplasm, nucleus, and other basic organelles. In addition, the creature appears to be capable of using oxygen in the course of its metabolism. Following a rigorous biochemical analysis, you note the following points: a. A glycolysis-like process begins with the alien's basic food stuff of sand particles. Specifically, the first molecule in this pathway is a silicon-256 atom (Si256). b. In the process of metabolizing (breaking down) the Si256, you note that the creature uses up 50 energy- carrying molecules that you have named ZTP (an unknown triphosphate-bearing molecule that gives the creature 'zip'). The Si256 is converted to 4 molecules of Si64. Also, in the process of breaking down 1 molecule of Si256, 10 molecules of an electron-carrying substance you have called EADH are formed. c. Each Si64 molecule is broken down into two molecules of Si30. During this step, 5 ZTP molecules and 4 EADH molecules are produced for each Si64 molecule broken down. Also, during this step, each molecule of Si64 gives off 4 SiO2 molecules. d. Each Si30 goes through a cyclic process in an organelle similar in structure to our own mitochondrion. In one turn of the cycle (for 1 Si30), 8 ZTP, 4 EADH, 7 EADH2 and 2 SiO2 are produced. e. In the final stage, each molecule of EADH is converted to 10 ZTP, and each molecule of EADH 2 is converted to 5 ZTP. How many ZTP and SiO2 molecules will be formed (net totals) in the complete breakdown of 1 molecule of Si256? Produce a simple flow chart in the space below showing what you start with and what you end up with in each step to help you get organized.