COURSE BSP 1 0 1 Microbiology TITLE S. No. Description PREPARATION 1 Safety guidelines , Risk assessment, Good microbiological laboratory practice (GMLP) Spillage management, Aerosols Introduction to instrumentation of microbiology lab (working of autoclave, laminar flow bench, hot air oven, BOD incubator, etc.). MEDIA, STERILIZATION AND DISINFECTANTS 2 Preparation of culture media, Storage of media, Sterilization vs. disinfection, Use of the autoclave, Sterilization of equipment and materials, Choice, preparation and use of disinfectants Structure and function of compound microscope INOCULATION AND OTHER ASEPTIC PROCEDURES 3 Essential points, Using a wire loop, Using a pipette, Flaming the neck of bottles and test tubes, Streak plate, Pour plate , Using a spreader, Spread plate 4 Simple, negative, capsule, acid fast and gram staining of microorganisms. 5 Negative staining of microorganisms 6 Capsule staining of microorganisms 7 Acid fast and gram staining of microorganisms 8 Determination of viability & counting of microorganism Suggested Readings : 1. S. M. Ali ; Practical Manual Of Biotechnology; 2009 pointer publishers 2. A.K. Roy,.; Laboratory Manual of Microbiology: Practical Manual Series: 05; 2010 Emanuel Goldman, Lorrence H Green; Practical Handbook of Microbiology, 2nd Ed; CRC Press 4. Bergey’s Manual of Determinative Bacteriology; Peter Henry Andrew Sneath, Nicholas S. Mair, M. Elisabeth Sharpe; 1986 Lippincott Williams & Wilkins 3. COURSE BSP 1 0 2 Cell Biology TITLE S. No. 1 Description Microscopy: Principles of compound, phase contrast & electron microscopy (SEM & TEM). Use & care of light and compound microscope 2 Study of permanent slides of various cell organelles Plasma membrane, mitochondria, golgi complex, lysosomes, endoplasmic reticulum (SER+RER), cilia, centriole, 3 Preparation of permanent slides: Section cutting of plant tissues and their staining 4 Chromosome staining & DNA Staining 5 Study of Cells: Lactobacilli, E. Coli, Sacharomyces, Blue green algae, 6 Study of Permanent slides of Mitosis & Meiosis Sugested Reading : 1. John Davey and Mike Lord; Essential Cell Biology, Volume 2: Cell Function, A Practical Approach; 2003; Oxford University Press. 2. S. Harisha; An Introduction to Practical Biotechnology; 2006; Luxmi Publishers, India COURSE BSP 1 0 3 Inorganic Chemistry TITLE S. No. Description 1 To prepare different molar / normal solutions of NaOH and HCl i.e N/10, N/20 and N/50 2 Determine the strength of given NaOH solution volumetrically 3 Determine the strength of given HCl solution volumetrically. 4 Determine the morality and strength of given KMnO4 solution by titrating it against M / 50 oxalic acid solution Suggested Readings: 1. Practical Chemistry, B.Sc Part I., S.Kiran. 2. Vogel's qualitative inorganic analysis, Arthur Israel Vogel, G. Svehla 3. Practical Chemistry, University Press, By V.K. Ahluwalia. COURSE BSP 1 0 4 Organic Chemistry TITLE S. No. Description Determination of melting points: Naphthalene, 80-820. Benzoic acid, 121.5-1220 1 Urea, 132.5-1330, Succinic acid, 184.5-1850. Cinnamic acid, 132.5-1330, Salicylic acid, 157.5-1580. Acetanilide, 113.5-1140, 2 Determination of boiling points Ethanol, 780, Cyclohexane, 81.40. Tolune, 110.60, Benzene, 800. Crystallization 3 1) 2) 3) 4) 5) Concept of induction of crystallization Phthalic acid from hot water (using fluted filter paper and seamless funnel) Acetanilide from boiling water Naphthalene from ethanol Benzoic acid from water Suggested Readings: 1. Practical Chemistry, B.Sc Part I., S.Kiran. 2. Vogels Practical Organic Chemistry-Quantitative analysis Part-III COURSE BSP 1 0 5 Physical Chemistry TITLE S. No. 1 2 Description To determine the pH of give acid and base solutions To determine the surface tension of given liquids (i.e. CCl4) using stalagmometer by drop number method. 3 To determine the surface tension of given liquids (i.e. glycerine) using stalagmometer by drop number method. 4 To determine the coefficient of viscosity of the given liquid using Ostwald viscometer. Suggested Readings: 1. Modern Approach to Physical Chemistry Practicals, B.D Khosla. 2. Practical Chemistry, B.Sc Part I., S.Kiran. 3. Advanced Practical Physical Chemistry, By Dr. J.B.Yadav. 4. Practical Chemistry, University Press, By V.K. Ahluwalia. COURSE BSP 1 0 6 TITLE Professional Communication Skills S. No. Description 1 Verbal Skills -Art of Self Introduction, Greetings, Asking for things, Inviting, Congratulating and Apologizing. Art of Complimenting, Telephone Etiquette, Roleplays. 2 Non-Verbal Skills- Positive body language, posture, gestures, symbols and signs, Personal Appearance and the art of self-presentation & conduct. Developing positive personal attitudes 3 Listening Skills- Answering questions to passages after listening, English Songs 4 Art of Public Speaking: Newspaper Reading, Extempore, Just a Minute, Group Discussion 5 Presentation Skills: Principles of Oral presentations, Strategies for improving oral presentations, Presentation on a technical topic by the student 6 English Grammar: Workbook-Noun, pronoun, verb, adjective, adverb, preposition, article and conjunction 7 Vocabulary Building- Synonyms, Antonyms, Homonyms, Homophones, Analogy, Abbreviations, Technical Terms, difficult spellings 8 Sentence syntax- Tenses, Punctuation, Active & Passive voice, Reported Speech, Idioms, Correction of sentences, rearranging of sentences in paragraphs and transformation of sentences. Suggested Readings : Textbook: 1. Chandigarh University-Professional Communication Skills – Workbook 2. Chandigarh University - Professional Communication Skills -Study Material 3. Sadanand, K., Spoken English part 1 (CD's) 4. Murphy, Raymond., Elementary Grammar (Intermediate Level), Cambridge University Press (2012 5. L- Client Software for Listening Skills Reference Book: 1) Hewings, Martin, Advanced English Grammar, Cambridge University Press (2011) 2) Lewis, Norman., Word Power Made Easy (2012)