BI 234: Microbiology
Lab Syllabus
Spring 2015
Instructor: Corrie Kezer
Office: RVC HEC 301-F
Phone #: 541-245-7680
E-Mail: ckezer@rougecc.edu
Required Materials: *Leboffe and Pierce, Microbiology: Laboratory Theory and Application, 3rd Ed.
*Safety Goggles. Available for purchase in the RCC bookstore. If you want to purchase goggles
outside the RCC bookstore, they must meet the ANSI Standard Z87.1-1989/2003 (Chemical
Goggles, Polycarbonate Lens with indirect venting)
*Gloves. At least 1 pair per lab day, more is recommended (just in case)
Date
Lab Activities
Quiz #
Week 1
Week 2
Week 3
Week 4
Week 5
3/31
1.3 “Aseptic Technique”, 2.1 “Ubiquity of Microbes”
4/2
results from previous labs, 6.1 “Plate Counts”, 1.4 “Streak Plates”
4/7
results from previous Labs, 2.2 “Colony Morphology”
4/9
3.1 “Light Microscope”, 3.3/3.4 “Eukaryotic Microbes”/”Pond Water”
4/14
3.5 “Simple Stain”, 3.6 “Negative Stain”
4/16
3.7 “Gram Stain”
4/21
3.8 “Acid-Fast Stain (Ziehl-Neelsen Method)”
4/23
3.9 “Capsule Stain”, 3.10 “Endospore Stain”
4/28
4.2 “Sheep Blood Agar”, 4.4 “MSA”, 4.6 “EMB Agar”,
5.4 “MR-VP Tests”, 5.3 “Phenol Red Broth”
4/30
results from previous labs, practice stains
5/5
Week 6
5/7
Week 7
Week 8
Week 9
5/12
results from previous labs, Handout “Unknown Identification” Day 1
5/14
10.3 “Bacterial Transformation” and “Unknown ID” Day 2
5/19
results from previous lab, “Unknown ID” Day 3, 2.9 “Temperature
and Growth”
5/21
results from previous lab, “Unknown ID” Day 4,
5/26
7.3 “Kirby-Bauer Test”, 2.13 “UV Light and Growth”
5/28
6/2
Week 10
Week 11
5.20 “SIM” 5.12 “Starch Hydrolysis”, 5.13 “UreaseTest”,
5.14 “Casease Test”, 5.15 “Gelatinase Test”
results from previous labs, 5.5 “Catalase Test”, 5.6 “Oxidase Test”,
5.7 “Nitrate Reduction”, 5.8 “Citrate Test”,
results from previous lab, Handout: “Soil Antibiosis Lab”,
Handout: “Chemical Control”, “Unknown ID” Project Due
Soil Overlay, 7.6 “Epidemiology”, 8.9 “Bioluminescence”
6/4
results from previous lab, stain soil microbes, Handout: “Making
Yogurt”
6/9
Lab Practicum
6/11
Results from Practicum and finish “Yogurt Lab”
No Quiz
Quiz 1
Quiz 2
Quiz 3
Quiz 4
Quiz 5
Quiz 6
Quiz 7
Quiz 8
Quiz 9
STUDENTS ARE RESPONSIBLE FOR ANY ANNOUNCED CHANGES IN THE SYLLABUS
GRADING:
9 Lab Quizzes (5 points each)
Unknown Identification Project
1 Lab Practicum
Total Lab Points Possible
45
25
50
120
MAKE-UP POLICY
There are NO make-ups for lab activities, lab quizzes, or the lab practicum. No late Unknown ID projects will be accepted
STATEMENT ON CHEATING
CHEATING AND PLAGIARISM ARE VIOLATIONS OF THE COLLEGE POLICY AND ARE CONSIDERED SERIOUS OFFENSES.
THE SCIENCE DEPARTMENT TAKES ALL INCIDENCES OF ACADEMIC DISHONESTY SERIOUSLY AND ACTS
ACCORDINGLY.
FOOD AND DRINK IN THE LAB
Absolutely no food or drink is to be consumed or stored in this lab at any time unless directed by your instructor. This
also applies to cosmetics, gum, etc.
SAFETY POLICY
See the lab safety rules document and student safety contract discussed in lab. No gloves or goggles, no participation in
the lab…NO EXCEPTIONS!!