IACUC NO.
CALIFORNIA STATE UNIVERSITY, DOMINGUEZ HILLS
INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE
For IACUC Use Only
N
O
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FORM A
Project Title:
CURRENT APPROVAL PERIOD:
From:
Until:
Funding Source:
Principal Investigator (Last, First, Position/Title):
Department:
Phone:
Email:
Co-Investigator (Last, First, Position/Title):
Department:
a If
1.
Phone:
Email:
new, attach copy of grant proposal; project titles and procedures must be consistent.
NATURE OF THE PROTOCOL/STUDY Indicate (x) all applicable items.
Research Project
Blood/Tissue/Embryo Collection
Survival Surgery
Teaching
Breeding Program
Multiple Survival Surgery a
Behavioral Studies
Neuromuscular Blocking Agents
Terminal Surgery
a
b
Antibody Production
Radioactive Materials
Prolonged Restraint (> 1 h) a
a
b
Mouse Ascites Production
Biohazardous Materials
Food or Fluid restriction a
Tumor Inducement a
Other Hazardous Agents b
Transgenic animal use
a For special procedures, please ensure that you have consulted with CSUDH regulations regarding these procedures and
incorporated them into the body of this protocol.
b Requires pre-approval by Environmental Health and Safety Office.
2.
TYPE OF PROTOCOL STATUS
New
Renewal of previously approved 3-year protocol
3.
EXPERTISE OF PRINCIPAL INVESTIGATOR
4.
PROJECT PERSONNEL Provide the names, titles, and relevant training of all participants who will handle the animals
for the duration of the project. Include dates of completion of required CSUDH Animal Use Training Program.
(Name)
(Title)
Describe briefly your qualification to perform the proposed experiments.
(Prior Training)
(Completion of Training Program)
(Name)
(Title)
(Prior Training)
(Completion of Training Program)
(Name)
(Title)
(Prior Training)
(Completion of Training Program)
(Name)
(Title)
(Prior Training)
(Completion of Training Program)
Form A--Page 2
5.
IACUC NO.
ANIMALS REQUESTED
Species
(including
sex, age, &
any other
desired
traits)
Strain
Animal source
(Commercial/n
on-Commercial
or field location)
Number of
animals
requested for
entire project
Locations
where
animals will
be housed
(vivarium and
individual
faculty labs)
Approval of
Animal Care
Department
Director
Pain/Distress
Type*
C
D
E
* List each pain/distress type separately. Count each animal only once for the highest category.
Type C: Those in which pain or distress will not be induced. Animals will be utilized for collections, injection, or similar
procedures causing minor discomfort and no drugs will be used.
Type D: Those that induce pain or distress that will be relieved by the use of appropriate drugs. Indicate the analgesics
and/or anesthetics to be used. Also, indicate whether and how you will monitor the animal for signs of pain or discomfort.
Type E: Those that involve pain or distress not relieved by drug intervention. These procedures are permitted only where
drug intervention would interfere with the results of the experiment.
Field Collections:
For each species to be collected from the field, describe the method of collection. Include copy of Dept. of Fish & Game
Collection Permit, if required, and procedures used to ensure that field studies do not compromise the health and safety of
animals or persons working in the field.
6.
PROCEDURES AND METHODS OF THE STUDY
Justification for animal study:
In clear, concise, non-technical language, summarize the background and specific aims of your STUDY and the relevance
of the proposed research to human or animal health and/or to the advancement of scientific knowledge.
Description of animal procedures:
Describe each procedure that directly involves animals (including, but not limited to: surgery, blood sampling, pain control,
injections, and restraint of animals for handling). List procedures in the order in which they will occur; note where
procedures will be repeated (and indicate number of repetitions); and, where appropriate, indicate the duration of
procedures (e.g., exposure to chemical agents, restraint), pre- and post-procedural monitoring, and the final disposition of
all animals included on each project.
Describe anticipated effects of these procedures on animal health, including potential adverse effects on health/behavior.
Form A--Page 3
IACUC NO.
Describe how these effects will be monitored for and under what conditions animals will be prematurely removed from the
study and euthanized (due to injury, illness, or other unexpected adverse effects).
Include a timeline for the experiment, accounting for all procedures and animals.
Additional information:
For animals exposed to radiation and/or biohazards and/or other hazards, please list hazardous agent, animal dosage
or exposure, and any precautions that will be taken (including proper training of all participants who will handle radioactive
and/or biohazardous material). Note: Animal Protocol approval will not be granted for studies involving radiation,
biohazards or other hazards until the use of such agents is approved by the Environmental Health and Safety Office.
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IACUC NO.
For animals that will undergo survival surgical procedures, please complete the following items:
Location (building/room number, or off campus site)
at which surgery will be performed:
Preoperative medications and anesthesia (include
agent, dose, route and frequency of administration):
Paralyzing drugs used, if any (include agent, dose,
route and frequency of administration):
Criteria used to assess the level of anesthesia:
Respiration rate
Positive toe reflex
Heart rate
Other, explain:
Corneal reflex
Typical duration of survival surgery:
Postoperative analgesia (include agent, dose, route
and frequency of administration).
Describe postoperative observation (include
frequency of monitoring and any medications
[include agent, dose, route and frequency of
administration]):
7.
PROCEDURES EMPLOYED You must categorize each procedure as Type C, D, or E.
Type C: Those in which pain or distress will not be induced. Animals will be utilized for collections, injection, or similar
procedures causing minor discomfort and no drugs will be used.
Type D: Those that induce pain or distress that will be relieved by the use of appropriate drugs. Indicate the analgesics
and/or anesthetics to be used. Also, indicate whether and how you will monitor the animal for signs of pain or discomfort.
Type E: Those that involve pain or distress not relieved by drug intervention. These procedures are permitted only where
drug intervention would interfere with the results of the experiment.
Procedure
1
Description
Number of
animals
requested for
entire period
Type C
Type D1
Type E2
For each procedure listed above classified as Type D, the analgesics and/or anesthetics to be used should be indicated
in the surgical procedures section in question 7.
2
For each procedure listed above classified as Type E, provide evidence for the claim that drug intervention would
interfere with the results of the experiment. (For example, indicate the relevant literature that you consulted, showing that
the Type E procedure is justified).
Form A--Page 5
IACUC NO.
For all procedures described above, indicate what criteria will be used to assess pain/discomfort of animals:
Loss of appetite
Loss of mobility
Weight loss
Vocalizing
Failure to groom
Licking
Scratching
Biting
8.
RATIONALE AND ALTERNATIVES TO THE USE OF ANIMALS
Federal regulations require that all investigators provide a short narrative describing the rationale for using animals, the
appropriateness of the proposed species, and the methods and specific sources used to determine that the alternatives
are not available. Your narrative must address the following:
For classroom instruction, state the desirable skills or information being taught to students through the use of animals.
Explain why animals are required for your studies. If there is an alternative non-animal model such as mathematical
model, cell line, or computer simulation, explain why you are not using it.
Explain why the proposed species is/are the most appropriate.
Explain how you derived the number of animals requested based on the number of experimental groups, animals per
group and data points required to allow statistical evaluation of your results.
Describe the steps you have taken to reduce the number of animals in your study (e.g., by refining experimental design or
replacing use of animals with in vitro procedures).
Using the table below document your literature search which you have performed to determine all of the following: (a) your
proposed research is not unnecessarily duplicative, (b) alternatives to animal research are not appropriate, and (c) for all
category D and E studies, alternatives to the use of procedures that may cause more than momentary or slight pain or
distress are not appropriate or available. You must have consulted at least 2 databases. Some recommended sources
are: National Library of Medicine’s databases and electronic information sources
(http://wwwcf2.nlm.nih.gov/nlm_eresources/eresources/search_database.cfm ), which include Medline,
Animal Welfare Information Center (AWIC) (http://awic.nal.usda.gov/), National Agricultural Library’s AGRICOLA
(http://agricola.nal.usda.gov/), University of California Center for Animal Alternatives
(http://www.lib.ucdavis.edu/dept/animalalternatives/), Current Research Information System (cristel.nal.usda.gov8080/),
Toxline (available through http://toxnet.nlm.nih.gov/cgi-bin/sis/htmlgen?TOXLINE), and National Association For
Biomedical Research (www.nabr.org).
Search site
Date of search Years covered
Keywords
Number of hits*
*If your search returns hits comment here on possible duplication, overlap, and alternatives (see a, b, and c above):
Form A--Page 6
9.
IACUC NO.
ANIMAL DISPOSAL Indicate the method of disposal of the animals used.
Please describe the intended final disposition of the animals in your study.
Euthanasia
Method of Euthanasia
(Acceptable Methods by Species per Dosage, Route of Administration
Species
2000 AVMA Panel on Euthanasia)
and Method to Ensure Death3
Mice
Barbiturates
Rats
Inhalant Anesthetics
Other small rodent: _______
CO2
Rabbits
KCl with general anesthesia
other : ___________1
Birds
Barbiturates
Inhalant Anesthetics
CO2
other : ___________1
Free ranging wild life
: __________
Barbiturates IV or IP
Inhalant Anesthetics
KCl with general anesthesia
other : ___________1
Amphibians
Frogs
Toads
Other: ___________
Barbiturates
Inhalant Anesthetics2
CO2
Double pithing
Tricaine methane sulfonate
Benzocaine hydrochloride
other : ___________1
Fish
Barbiturates
Inhalant Anesthetics2
CO2
Tricaine methane sulfonate
Benzocaine hydrochloride
other : ___________1
Reptiles
Barbiturates
Inhalant Anesthetics2
CO22
1:
Other methods of anesthesia must be justified.
In appropriate species.
3: For inhalant anesthetics and CO2, cervical dislocation is recommended for small rodents and birds.
2:
Other, explain:
REMINDER: If a protocol has been terminated, please notify the IACUC Coordinator via email.
Form A--Page 7
IACUC NO.
10. INVESTIGATOR’S ASSURANCE
I, as principal investigator, agree to abide by the CSUDH Institutional Animal Care and Use Committee (IACUC) policies
and procedures, the provisions of the NIH Guide to the Care of Laboratory Animals, and all federal, state, and local laws
and regulations governing the use of animals in experimentation.
I have considered the alternatives to the use of animal models and painful or distressful procedures used in this project
and found these alternatives unacceptable (or have justified the use of animals and/or painful or distressful procedures
where alternatives exist).
I declare that the protocol described does not unnecessarily duplicate previous work or experiments.
I declare that all experiments involving live animals will be performed under my supervision or under that of another
qualified scientist. Technicians and students involved have been trained in proper procedures in animal handling and in
any administration of analgesics/anesthetics, animal surgery, and euthanasia to be used in this project. The training also
covered animal handling related health risks.
I further declare that the information provided in this Request to Use Vertebrates protocol is accurate to the best of my
knowledge. Any proposed revision to the details listed under this Protocol will be promptly transmitted in writing to the
committee for approval prior to implementing any changes.
11. CERTIFICATION
Principal Investigator’s Signature
Date
Department Chair’s Approval
Date
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IACUC NO.