CURRICULUM VITAE SAIFULLAH KHAN PERSONAL DETAILS Father Name: Date of Birth: Marital status: N.I.C. Number: Citizenship: Present Position: Fayyaz Ali Khan July 01, 1967, Bahawalpur (Pakistan) Married 42501-2782657-5 Pakistani Associate Professor and Incharge, Biotechnology Section, International Center for Chemical and Biological Sciences, H.E.J. Research Institute University of Karachi 75270 Fax: (9221) 4819018-9 Telephone (92-21) 99261693, E-mail: drsaif65@gmail.com Res: House No. A 46, Hasrat Mohani Colony Munghu Peer Road Karachi. Telephone 92-21-32578547 Mobile: 0300 2375938 ACADEMIC PROFILE QUALIFICATIONS Postdoctoral 2009-2010 Ph.D. 1994-1997 M.Sc.(Hons)* Agriculture 1992 B.Sc. (Hons)** Agriculture 1985-1989 H.S.C. (Pre-Medical) 1984 S.S.C. Matriculation 1982 MARKS / CGPA / %age DISTINCTIONS Fulbright Fellowship Ministry of Education Merit Scholarship 3.69/4.00 (77.20%) Merit Scholarship INSTITUTION AND SUBJECTS Applied Biotechnology Institute.(ABI) Cal Poly State University, California USA University of Edinburgh UK. Plant Biotechnology University of Agriculture, Faisalabad Plant Breeding and Genetics 3.60/4.00 (75.39%) Merit Scholarship (59.9%) ---------- University of Agriculture, Faisalabad Barani College Faculty Plant Breeding and Genetics Board of Intermediate and Secondary Education Bahawalpur Merit Scholarship Board of Intermediate and Secondary Education Multan 75.76% DISSERTATION TITLE Postdoc Training: Pharmaceutical and other important proteins production in Plants using recombinant DNA technology. Ph. D. Variation arising through tissue culture in Soft fruit species. M. Sc. Hons. Gene action studies of some morphological and other important traits in cotton (Gossypium hirsutum L). WORK EXPERIENCE: Institution International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry University of Karachi Karachi 75270 Position held Period Main duties From To Associate 19th July Professor 2008 till now Same as above Same as above Assistant 26th May Professor 2001-19th July 2008 Supervising post graduates research students Teaching postgraduates students at the institute and department of biotechnology and agriculture Karachi University as a visiting faculty. Writing grants for national and international funding Executing various projects as P.I. Same as above Senior Nov 97 to Research May 2001 Officer University of Research March-1994 Edinburgh Kings Scholar to Oct 1997 Buildings West Mains Road Edinburgh UK Established largest plant tissue culture facility in the country Optimize in-vitro protocols and scaling them up for commercial utilization. Writing various grant proposals , Teaching postgraduate students as a visiting faculty in Karachi University Various research assignments Ph.D. project planning and execution Theses Faculty incharge Plant Biotechnology Section at the International Center for Chemical and Biological Sciences (ICCBS), H.E.J. Research Institute, University of Karachi Pakistan. Established an efficient and most economical plant cell and tissue culture facility, starting from lab designing, refurbishing, establishing tissue culture protocols of various plant species. This becomes the largest tissue culture facility in the country. Cell Culture Facility: Developed various ornamental as well as medicinal plants calli and cell suspensions cultures for the study on secondary metabolites and biotransformation. These include Kiwifruit, Aloevera, mint spp. Ocimum, Neem, Croton, Hermal, Euphorbia, Catharanthus spp. etc. Floriculture: Established Green net houses and grew successfully exotic cut flower like Orchids and Anthurium, now this is first and the only pilot scale Orchid and Anthurium cultivation farm in the country based on indigenous fertigation protocols develop by us. Production of field cut flower, Tube roses, Roses, Jasmines and Hibiscus tea. 2 Hydroponics Working on Hydroponics and off-season production of economically important exotic high value horticultural crops like piper betal and cherry tomatoes. Established first and the only Anthurium cut flower pilot scale farm” in the country based on hydroponics. Formulate various foliar fertilizer based on local salts to use them in hydroponics. These include special plant foods names Greener, Bloomer, Stress supplement and Magic greener. Extraction of essential oils using supercritical and liquid Carbon di oxide technology from various medicinal plants and flowers. TEACHING EXPERIENCE 2007 – 2008 Teaching courses in the Department of Agriculture, University of Karachi as a visiting faculty. Feb. 1998 - 2005 Taught number of postgraduate courses in the department of biotechnology, University of Karachi. These includes plant tissue culture and regeneration, agriculture biotechnology, fresh water fish farming, hydroponics. RESEARCH TECHNIQUES AND SKILLS: Various molecular techniques are routinely in use in the lab. These include:DNA Fingerprinting PCR (Polymerase Chain Reactions), AFLP (Amplified Fragments Length Polymorphism), Microsatellite SSR (Simple sequence Repeats) and PCR based disease diagnostics and RAPD PCR, (Random Amplified Polymorphic DNA). SDS-PAGE and Starch Gel Electrophoresis for Protein and Isoenzyme Electrophoresis. A very efficient and economical silver staining protocol has been developed in the lab using basic salts. Western blotting. Recombinant DNA Technology Various molecular techniques like DNA cloning, designing vectors for transformations Recombinant DNA technology, transformation using Biolistic gun, Electroporation and Agrobacterium. Used ELISA in routine as a diagnostic tool to index plants ELISA is also being used to detect the expression level of a transgene. Plasmid and nuclear DNA extraction from various parts of plants and calli and bacteria using different protocols including "mini and maxi prep." Biotransformation and protoplast cultures Cell suspension culture and biotransformation technology. Protoplast isolation and somatic hybridization. Somatic embryogenesis is routinely being used in the lab as an alternative method of propagation. 3 Essential oils Isolation of essential oils using state of the art technique Supercritical Carbon dioxide technology and steam distillation. Computer skills Software like Microsoft Office, Statistical packages for analysis of data (Statgraph, Minitab), Gel documentation system. Molecular data analysis programs, RAPDistance, Phylip 3.5 and cloning software of “Vector nti “other routine programs. ADMINSTRATIVE EXPERIENCE: Provost post graduate Boys hostel of ICCBS 2005-2009. Member purchase committee of the ICCBS since 2004-2009. Involve in Organizing various International Workshops, Symposia, Conferences and organized more than 20 International events in the Institute as member organizing committee. MEMBERSHIP OF SCIENTIFIC ORGANISATIONS AND HONOURS: Chawala Kissan Time award for Best Tissue Culturist in Pakistan 2004. Member of the Society for in-vitro Biology, USA. (SIVB). Member of the Genetical Society, UK. Member of the International Society for Horticulture Sciences (ISHS), Belgium. Life-time member of the Horticulture Society, Pakistan. Appointed and working as an external examiner for conducting viva voce examination for Ph.D. candidates in School of Biological Sciences, Punjab University, Lahore, Pakistan. Member, Banana Research Information System (BRIS), Belgium. Life time member of the Pakistan Botanical Society. Life time member of Bazm-e-Science a Adab. Participated as an active member Pakistan National Committee for International Union for Conservation for Natural resources (IUCN) HEC Text Book reviewer on Plant Biotechnology. Technical reviewer of various research projects funded by various Governmental Organizations such as Higher Education Commission, Pakistan, Science Foundation, Ministry of Science and Technology, and Pakistan Telecommunication Limited. Technical evaluator of Manuscripts of various Scientific and Industrial Research journals such as University of Balochistan, Quetta, Pakistan Journal of Agriculture, Agriculture Engineering and Veterinary Science, Tandojam and Pakistan Journal of Botany. 4 MEMBER BOARD OF STUDIES Working as an Adviser for Pakistan Biotechnology Information Center (PABIC). Worked as member Board of Advance Studies, Center for Advance Studies in Biotechnology at Sindh University, Jamshoro. Worked as member Board of Studies of the Department of Biotechnology, Sindh Agriculture University, Tandojam. Member of various groups on specific crops constituted by The Agriculture Department Government of Sindh. STUDENTS SUPERVISED. A number of postgraduate student are working for various program under my supervision. Until now two students has completed and have been awarded Ph.D. degrees. Ph. D. COMPLETED: No. 1 2 Name of student Dr. Bushra Ahmad Saeed Dr. Saifullah Title of Ph.D. project In-vitro Selection and Evaluation of the Genetic Purity of Regenerated Banana Plants. Biotransformation of commercially and medicinally important natural products using plant cell suspension cultures. Awarded in 2007 2009 MPhil/MS/Ph.D. STUDENTS: No. 1 2 Name of student Mrs. Asma Nasib Title of synopsis Conversion Production of secondary metabolites from 2005 medicinally important plants using plant tissue culture technology. In-vitro propagation, differentiation and 2007 Mrs. Mariam Abdul acclimatization of Fern, Asplenium Nidus. Raziq 3 Mrs. Zarreen Badar 4 Ms. Sheeba Naz 5 Ms. Naheed Kauser 6 Mr. Hammad Afzal Kayani Enhanced production of Aloemannan and other constituents using Plant Biotechnology. Application of Plant Cell Cultures for the biotransformation of Terpenes. Production of Therapeutic agents from Plants using DNA recombinant technology. Biotransformation of Terpenes using Medicinal Labiates and fusion effect on 5 2009 2008 2007 2008 Work status Ph.D. bench work completed. (writing) Ph.D. bench work completed (writing) Ph.D. in progress. Ph.D. in progress Ph.D. in progress Ph.D. in Progress 7 Mr. Abdul Rehman 8 Ms. Tabassum BiBi 9 Ms. Syeda Kahkashan Kazmi the products. Transformation of sugar cane (Saccharum officinarum) for economically important gene using recombinant DNA technology. Molecular characterization of in vitro propagated date palm. Study on the development of seedless Kinnow (Citrus). 2009 Ph.D. in progress 2009 Admission 2009 Admission MS/ Ph.D. in program MS/ Ph.D. in progress M.Sc. RESEARCH THESES : No. 1 Mr. Hammad Afzal Kayani In-vitro studies of ferns Work completed 2004 2 Mr. Kashif Ali In-vitro studies of croton (Codieaum variegatum) 2004 3 Ms. Ambreen Ansari In-vitro studies of banana and some ornamental plants 2003 4 Ms. Mussarat Ashraf 5 Name of student Title of theses In-vitro studies of date palm (Phoneix dactylifera L.) and 2003 some ornamental plants Ms.Mehreen Gulsher Biotransformation of Dehydroepiandroestrone using cell 2007 suspension culture of Codiaeum varigatum. 6 Ms. Sabeen Habib 7 Ms. Salma Javed Biotransformation of Dianabol by cell suspension culture 2007 of Azadirachta indica. Biotransformation of Dehydroepiandroestrone using cell 2007 suspension culture of Azadirachta indica. M.Sc. RESEARCH PROJECTS: No. Name of student 1 Mr. Mudassir Nazir 2 Ms. Samreen Zehra Title of projects Work completed Evaluation of different media for the micropropagation of 2002 economically important plants. Evaluation of various alternatives to make Plant Tissue 2002 Culture more economically viable. 6 SHORT TRAINING AND COURSES 2010 Fulbright postdoctoral fellowship at Calpoly State University San Luis Obispo California USA, on protein production in plants using recombinant DNA technology, March 2009Feb. 2010. 2008 Training on ‘the use of somatic cell hybridization and transformation technology for the production of seedless citrus’ at National Key Lab. for Citrus Molecular Breeding Guangzhou Agriculture University, China, March 15-May 21. 2007 Participated in A hand on training program in supercritical carbon di oxide extraction at Loyalist College, Bellevill, Ontario Canada, May 9-11. 2007 Training on ‘the use of supercritical system and its use in food and essential oils extraction’ at Lehigh University Pennsylvania, USA, May12- June 8 2007 Participated as an Advisor in an International media workshop on Innovative aspects of Biotechnology and its better awareness and Dissemination in Islamabad, Pakistan, March 12-14. 2007 Participated on behalf of Director PABIC in the Annual meeting of the International Biotechnology Information Centre supported by ISAAA in Jakarta, Indonesia, Feb. 7-8. 2006 Participated in International HortiFair and visited Anthura B.V., Production Houses at Amsterdam, the Netherlands, Nov. 1-6. 200 2005 Visit to Ashu Flower Limited for sharing the experience of large scale commercial floriculture farm and their management in Thika, Kenya, Dec. 20-Jan 06. 2005 International workshop on Plant Biotechnology, A New promise for sustainable development at National Research Centre (NRC) in Giza, Egypt, December 3-7 2005. 2005 International training course on Plant Tissue Culture and Transformation Techniques at Agriculture and Genetic Engineering Research Institute (AGERI) in Giza, Egypt, April 213. 2004 Participated in WESCANA (West, Central Asia and North Africa) Regional Conservation Forum (RCF)-IUCN meeting at Amman, Jordan, April 5-7. 2004 Visit to T.K. Nurseries and various other Orchid cut flower farms to share the experience of cut flower production and processing Pathumthani, Thailand, Feb. 7-13. 2003 Participated in 4-week training at Date palm and Olive Research Institute in Safax, Degashe, Tunisia, Oct. 13- Nov. 03. 2003 Participated in the 1st International Symposium on Human Genome Organisation at Shangai, China, April 2-4. 2001 Actively participated in an International workshop on Novel Genetic Markers for crop improvement organised by International Centre for Genetic Engineering and Biotechnology (ICGEB) at University of Dhaka, Dhaka, Bangladesh, Nov. 4-13. 7 2001 successfully completed the training course on Latest developments in molecular biology and biotechnology held from Oct. 8-13 at UGC regional centre Lahore. 1998 Attended and successfully completed the ICGEB practical course on Application of Biotechnology in Agriculture Organized by ICGEB at NIBGE, Faisalabad, Pakistan, Feb. 16-27. 1997 Participated in workshop on Transposable Elements organized by Genetical Society at the University of Dundee, Dundee, U.K. 1996. Successfully completed a course on Radiation Protection in Laboratory work in Science and Medicine, Edinburgh, UK. 1996 Training at Long Austin Research Institute on The methodology of AFLP (Amplified Fragment Length Polymorphism) technique and its uses as genetic markers at Bristol, UK, Feb. 15-28. 1995. Attended Royal Society meeting on Control of Development in Higher Plants, London, UK. INTERNATIONAL TRAVEL. Various research institutes in the following countries were visited for training and conferences. 1. Bahrain 7. Tunisia 13. Netherlands 2. Bangladesh 8. Jordan 14. Saudia Arabia 3. Canada 9. Kenya 15. Thailand 4. China 10. Kuwait 16. Indonesia 5. Egypt 11. United Kingdom 17. United Arab Emirates 6. France 12. USA 8 PROJECTS EXECUTED Period 1998-99 Name of the Project Total Funds Commercial production of ornamental plants Rs.1.40 using Tissue Culture technology. million Funding Agency Status COMSTECH Completed P.I. 2002-03 Mass scale production of Pineapple plants using Rs.0.30 Tissue Culture technology. million MOST Completed P.I. 2002-04 Mass multiplication of virus and disease free Rs.6.85 Banana and Orchid plants using Tissue Culture million technology. MOST Completed P.I 2003-05 The use of DNA fingerprinting to evaluate the Rs.0.8 genetic stability of Banana plants produced via million in-vitro culture. PSF Completed P.I 2003-04 Establishment of model Hydroponics culture Rs.0.20 system for the cultivation of Pineapples and some million other important cut flowers. TWAS Completed P.I 2004-06 Establishment of Tissue Culture technology park Rs.14.90 in the University of Karachi. million HEC Completed P.I 2004-05 Establishment of medicinal plant collection and Rs.14.4 processing facility at New Murree million HEC Completed Co-P.I 20052006 Application of the Biotransformation techniques US$ to produce value added compounds using plant 7,250/cell cultures. EMRO Completed P.I. 20052007 Isolation of structurally novel and Rs.5.071 pharmacologically potent compounds from plant million cell suspension cultures. HEC Completed. Co-P.I. 20052007 Development of economical protocols for the Rs.1.648 pilot scale production of elite Date palm cultivars million using plant Tissue Culture technology. HEC Completed P.I 20052008 Establishment of research and development Rs.31.556 facilities for exotic cut flower and value added million products. HEC-CDWP Completed P.I. 20062008 Micropropagation and cultivation of some Rs.20.69 economically important plants. (Collaborative million Project at BUITMS, Floriculture Cell Governor House, Balochistan and ICCBS Karachi University). HEC Completed Co P.I 9 LIST OF PUBLICATIONS: SAIFULLAH AND KHAN, S. (2010) Callus induction and cell suspension culture production of catharanthus roseus for biotransformation studies of (–)-caryophyllene oxide. Pakistan Journal of Botany (Accepted). KIM, H,K., SAIFULLAH., KHAN, S., WILSON, E.G., KRICUM,S.D.P., SERGIO D. P., AXELMEISSNER, GORALOR, S., DEELDER, A.M., CHOI, Y.H. and VERPOORTE R. (2010). Metabolic classification of Ilex Species by NMR-based metabolomics. Phytochemistry, 71: 773-784 ABOUZAID S.F., EL-BASSUONY A.A, NASIB A, KHAN, S., QURESHI J, CHOUDHARY M.I (2010). Withaferin-A production by root cultures of Withania coagulans. International Journal of Applied Research in Natural Products 3(1), 23-27. KHAN, S., NAZ, S., KAUSER, N., RAZIQ, M., NASIB, A., AND SAEED, B. (2010). Cultivation of Lilies (sp. Lilium regale) for commercialization in Pakistan. Pakistan Journal of Botany ., 42(2). IKRAM-UL-HAQ, DAHOT, M. U., KHAN S AND KOUSAR N., (2009). Screening of banana bunchy top diseased plants a way to control its spreading Plant Omics Journal 2(4): 175-180. IKRAM-UL-HAQ, KHAN, S., DAHOT, M. UMAR, AND KAUSER, N. (2009). Efficient plant genomic and viral DNA isolation from mature midrib of Banana (Musa spp.). Journal of Plant Biochemistry and Biotechnology, 18 (2): 233-235. ATHER. A, KHAN, S., NAZIR, M., AND ABDUL REHMAN. (2009). Optimization of the Protocols for Callus Induction, Regeneration and Acclimatization of sugarcane cv. Thatta-10. Pakistan Journal of Botany ., 41(2): 815-820. NASIB, A., AND KHAN, S. (2009). Optimization and Establishment of Actinidia deliciosa cell suspension culture to study the biotransformation for the production of novel compounds. Pakistan Journal of Botany., 41(2): 835-841. NASIB, A., ALI, K., AND KHAN, S. (2008). An optimized and improved method for the In-vitro propagation of Kiwifruit (Actinidia deliciosa) using coconut water. Pakistan Journal of Botany, 40(6): 2355-2360. AZIZUDDIN, SAIFULLAH, KHAN, S. CHOUDHARY M. I. AND ATTA-UR RAHMAN (2008). Biotransformation of dydrogesteron by cell suspension culture of Azadirachta indica. Turkish journal of Chemistry, 32: 141-146. NASIRA, K., SHAHINA, F AND KHAN, S. (2008). Control of Pratylenchus Coffeae Root-Lesion Nematode in Ashok Tree using chemical amendment. Pakistan Journal of Nematology , 26(2): 175-186. KHAN, S., RAZIQ, M., KAYANI, H. A. (2008). In-vitro propagation and differentiation of Bird’s Nest Fern (Aspelinium Nidus). Pakistan Journal of botany, 40(1): 91-97. NASIB, A., ALI, K., AND KHAN, S. (2008). In-vitro propagation of Croton (Codiaeum variegatum). Pakistan Journal of Botany, 39(4): 1257-1262. 10 KHAN, S., NASEEB, S., AND ALI, K. (2007). Callus induction, plant regeneration and acclimatization of African violet (Saintpaulina ionantha) using leaves as explants. Pakistan Journal of Botany, 39(4): 1263-1268. KHAN, S., NAZ, S., ALI, K., AND ZAIDI, S. (2006). Direct organogenesis of Kalanchoe tomentosa (Crassulaceae) from Shoot tips. Pakistan Journal of Botany, 38(4): 977-981. NASIB, A., MUSHARRAF, S.G., HUSSAIN, S., KHAN, S., ANJUM, S., ATTA-UR-RAHMAN AND CHOUDHARY, M. I. (2006). Biotransformation of (-)-Ambrox by Cell Suspension Culture of Actinidia deliciosa. Journal of Natural Products, 69(6): 957-959. CHOUDHARY, M. I., SIDDIQUE, Z. A., KHAN, S., SAIFULLAH, MUSHARRAF, S.G. AND ATTAUR-RAHMAN. (2006) Biotransformation of (-)-caryophyllene oxide by Plant Cell Culture of Catharanthus roseus. Z. natureforsch. B,61b: 197-200. KHAN, S., NASIB, A., AND BUSHRA. S. (2005). Pineapple propagation: Pakistan’s new venture. Pineapples News, International Society for Horticultural Science, (12): 20-23. KHAN, S., AND SPOOR, W. (2004). A study of an in-vitro callus culture and regeneration system from leaf disc explants in strawberry (Fragaria ananassa) CV Tango. International Journal of Biology and Biotechnology, (3): 423-428. KHAN, S., IFTIKHAR, M., AND BUSHRA. S. (2004). An economical and efficient method for mass propagation of Ixora Coccinea. Pakistan Journal of Botany, 36(4): 751-756. KHAN, S., NAZ, S., AND BUSHRA. S. (2004). In-vitro production of cordylines terminalis for commercialization. Pakistan Journal of Botany, 36(4): 757-761. KHAN, S., NASIB, A., AND BUSHRA. S. (2004). Employment of in-vitro technology for large scale multiplication of Pineapples (Annanas Comosus). Pakistan Journal of Botany, 36(3): 611-615. KHAN, S., AND SPOOR, W. (2001). Use of molecular and morphological markers as a quality control in plant tissue culture. Pakistan Journal of Biological Sciences, 4 (4): 479-482. KHAN, S., AND SPOOR, W. (2001). Evaluation of genetic stability in the black currant plants regenerated via micropropagation using RAPD-PCR techniques. Pakistan Journal of Botany, 33(4): 411417. KHAN, S., ZAFAR, Y., YASMEEN A., AND BUSHRA, S. (2001). An efficient and economical method of mass multiplication of virus and disease free banana using plant tissue culture techniques. Pakistan Journal of Biological Sciences, 4(5): 562-563. KHAN, S., AND SPOOR, W. (2000). Evaluation of different explants sources and growth regulators on callus culture and regeneration in strawberry (Fragaria ananassa) Pakistan Journal of Biological Sciences, 3 (12): 2249-2250. 11 PAPERS AND PAPERS PRESENTED: Presented various talks at ABI (Agriculture Biotechnology Institute) Calpoly Technical State University, San Luis Obispo, California, USA. as a Fulbright postdoctoral scholar during my stay March 2009-Feb 2010. Organise the event as advisor PABIC launching of ISAAA brief: 39 press conference and scientific communication workshop on implementation of the strategy for development of biotechnology in Pakistan presented talk about present status of plant biotechnology at ICCBS. Feb. 27 -28 2009. Participated and presented talk on production of exotic cut flowers under green house conditions in Karachi, International workshop on protected horticulture organised by department of horticulture PMAS Arid Agriculture University, Rawalpindi, Pakistan. Feb. 27th - Mar. 1st 2008. Organised and participated in COMSTECH-ISESCO International workshop on innovative aspects of biotechnology and its better awareness and dissemination and presented talk about PABIC (Pakistan Biotechnology Information Centre) at Islamabad March 12 -14 2007. Participated in International symposium on prospectus of horticulture industry in Pakistan, organised by Institute of Horticultural Sciences University of Agriculture Faisalabad-Pakistan, March 28-30, 2007. Participated and organised “The concept of university-based technology parks in the light of global experiences organised by International Center for Chemical and Biological Sciences, University of Karachi, Commission on Science and Technology for Sustainable development in the South (COMSATS) and United Nation Educational Scientific and Cultural Organisation (UNESCO). Dec.10 , 2007. Participated in 3rd AFERP International days and 2nd Pak-France bi-national workshop agrocampus, and presented talks on Biotransformation of (-)-Ambrox using Actinidia deliciosa cell cultures at Agrocampus-Rennes cedex-France October, 26-30 2006 Role of Biotechnology work at H.E.J. Research Institute related to economic prosperity in the region in National Symposium on Biotechnology for economic prosperity organized by National Commission on Biotechnology and Ministry of Science and Technology at Nathiagali, Pakistan, 24-26 July, 2006. Attended and presented talk on Micropropagation of Anthurium andreanum, A boom in cut flower industry, in Two-day consultative workshop on R&D in Horticulture sector organized by Pakistan Horticulture and Developmental Board at Islamabad, Pakistan, 28-29 Nov., 2006. Attended and organized 9th International symposium on natural product chemistry organized by H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan, Jan. 10-13, 2004. Attended 1st International and National Symposium on Biotechnology and presented talks a: Effect of Coconut milk on micropropagation of Kiwifruit, Acinidia deliciosa at Jamshoro and b. In-vitro propagation and commercialization of Kalanchoe tomentosa, Jamshoro University of Sindh Pakistan, Mar. 22-23, 2003. Attended HEJ-COMSTECH (CPC)- TWAS International workshop on the development of medicines from plants jointly organized by the H.E.J. Research Institute of Chemistry COMSTECH (CPC) and the Third world Academy of Sciences Sept. 8-13, 2003. 12 Attended and organized 7th Eurasia conference on chemical sciences organized by H.E.J. Research Institute of Chemistry International Center for Chemical and Biological Sciences University of Karachi. Dec. 09, 2002. Attended and presented talk on Role of biotechnology for poverty alleviation in 4 day summer conference on poverty alleviation through sustainable agricultural development organized by the Department of Biotechnology, Agriculture University Peshawar at Baragali, Pakistan, July, 25-27, 2002. Attended and presented talk on “Use of molecular markers for quality control measures in commercially micropropagated cultivars of soft fruits’ in 4th International Plant Tissue Culture Conference at Dhaka, Bangladesh, November 1-3, 2001. Attended one day workshop on “The price of leather” social, political and economic costs of tanneries in Karachi held at the British Council, Karachi on Oct 17, 2000. Attended and presented talks on “The exploitation of plant tissue culture technology for large scale multiplication of disease and virus free plants” in Biotechnology for Development organized by COMSTECH-ISESCO-IDB-INOGE at Islamabad, Pakistan, January, 25-27, 2000. Attended and organised 7th International symposium on natural product chemistry organized by H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences University of Karachi. Dec. 28 1997 – Jan. 1, 1998. POPULAR LECTURES AND INTERACTION WITH THE MEDIA: Balochistan University of Information Technology and Management Sciences, Quetta: Biotechnology, Plant tissue culture technology its application for economic prosperity. Sindh University, Jamshoro: Eradication of banana bunchy top virus using tissue culture technology. Department of Microbiology, University of Karachi, Karachi: Cell and Tissue Culture Technology, its application in Research. Ladies Horticultural Club, Karachi: Plant Tissue Culture Technology, its application in Ornamental industry. Orchid Society of Pakistan, Karachi: Establishment of First National Orchid farm a successful story. Cactus Society of Pakistan, Karachi: Role of Plant Tissue Culture Technology in Propagation of Cactus and other endangered plant species. National Fertilizer Company, Thatta: Plant Tissue culture and Sustainable Banana production. Sindh University, Jamshoro: Role of Genetic Engineering (GMOs) in Gene revolution and food safety. 13 UAAR, Rawalpindi: Use of DNA finger printing technique to ensure genetic stability of Banana produced via In-vitro culture. Attended as an Expert Guest speaker and discussed key matters like Banana Bunchy Top Virus, Pineapple Propagation, Orchid Production, Hydroponics, Plant Diseases, Mushroom Cultivation, Banana Production Technology, and Role of Biotechnology in Poverty Alleviation in PTV-2, PTV, and PTV World Live and recorded and live in Razi Haider Shah program at KTN. 14