CURRICULUM VITAE ANDREA FICKE OFFICE Andrea Ficke Bioforsk Plantehelse Cereal crops Hogskøleveien 7 1432 Ås e-mail: andrea.ficke@bioforsk.no EDUCATION 1995 Vordiplom, Microbiology and Botany, Georg-August University, Goettingen, Germany 1996 Graduate school, Department of Plant Biology, Georg-August University, Goettingen, Germany 2002 Ph.D., Plant Pathology, Cornell University. Dissertation: Changes in the susceptibility of developing grape berries to Uncinula necator: Effect of ontogenic resistance on pathogen establishment and development, potential host barriers to infection, and host responses to inoculation and infection. HONORS 1996 Fulbright Scholar. A national competitive program of the US Department of State that brings citizens of other countries to the US for graduate study at U.S. universities. 1996 Foreign Student Scholarship of Cornell University. 1999 Eugene Saari Award. Awarded annually to support presentation of noteworthy research by graduate students at the national meeting of the American Phytopathological Society (see: Phytopathology 89:S25). 1999 - 2000 Mario Einaudi Center Scholar. Proposal: Molecular and anatomical aspects of ontogenic resistance to powdery mildew in grape vines. The Einaudi Center at Cornell University administers a competitive program to support graduate students conducting short-term research in countries outside the United State. 1 2001 I. E. Melhus Graduate Student Symposium Scholar. Proposal: Ontogenic resistance and plant disease management: A case study of grape powdery mildew. A competitive program in which four graduate students are selected each year from a worldwide pool to present their research at a special symposium at the annual meeting of the American Phytopathological Society (see: Phytopathology 92:671-675). 2002 Research Fellow, DAAD (Deutscher Akademischer Austausch Dienst). A competitive program administered by the German Academic Exchange Service to support post graduate training of German scientists at partner institutions. Proposal: Biosurfactant producing Pseudomonas to control Phytopthora in different crops with Dr. Jos Raaijmakers at Wageningen University. 2002 - 2003 EPS (Experimental Plant Sciences of the Netherlands) Postdoctoral Fellow. A competitive program to support postdoctoral research at partner institutions. Proposal: Responses of oomycete pathogens to metabolites produced by Pseudomonas flourescens with Dr. Jos Raaijmakers at Wageningen University. PROFESSIONAL EXPERIENCE 1996 Graduate assistant to Professor Gerhard Wolf at the Georg-August University, Goettingen. Research Project: Efficacy of biological control measures and pesticide applications for management of black stem rot of wheat. 1996-1998 Graduate student in residence, Department of Plant Pathology, Cornell University's main campus in Ithaca, NY, USA. Completed comprehensive coursework in Plant Pathology. Completed research training project with Prof. Eric Nelson: Extraction of root exudates from cottonseeds and evaluation of effects on Pythium ultimum. Additional training and work experience in Cornell's Plant Disease Diagnosis Lab. 1998-2002 Advanced to PhD candidate and joined Professor Robert C. Seem's laboratory at Cornell's Department of Plant Pathology at the New York State Agricultural Experiment Station in Geneva. Dissertation research focused on morphological, physiological and molecular factors involved in development of ontogenic resistance in grape berries. Methodology applied included brightfield, phase contrast, fluorescence, and confocal microscopy, scanning, environmental, and transmission electron microscopy. Developed methods for controlled inoculation of disease-free fruit in field studies of the nascent development of mildew colonies. Isolated RNA from grape berry skins and tested for induction of pathogenesis related proteins during infection of resistant vs susceptible berries. Outcomes of this research are now the basis of improved management programs for grapevine powdery mildew worldwide. 2 2002-2004 Post doctoral scientist at Department of Plant Pathology, Wageningen University, The Netherlands. In collaboration with Dr. Jos Raaijmakers, my work focused on the efficacy and mode of action of a surface tension reducing, zoosporocidal cyclic lipopeptide produced by P. fluorescens in controlling oomycete root and leaf diseases. This project involved in depth molecular work aiming to analyze so-called ‘biosurfactants’ and their effect on , including chemical analysis, microscopic observation and bioassays of disease in several field crops. 2007-2009 Pre-breeder for Cucurbitaceae at Rijkzwaan The Netherlands. Assessed breeding priorities and lead short and long term projects to improve disease and stress resistance, fruit qualities and plant architecture in melon, water melon, cucumber and pickles. 01.01.2009 -30.7.2009 01.09.2009 - currently Breeding manager of the Cucumber Department at Rijkzwaan B.V., The Netherlands. Overseeing and organizing research activities in cucumber breeding. Management of staff at the cucumber department. Continued prebreeding work in cucumber and melon. Cereal Plant Pathologist at Bioforsk Plant Health and Plant Division in Ås, Norway. Collecting and analyzing data on Leaf Blotch Diseases in Norway, their epidemiology, biology and the effect of changing climate on disease risk and impact on yield. Studying Fusarium Head Blight epidemics and toxin production. Utilizing recent and current data for disease forecasting models. ACADEMIC LEAVES Nov - Dec 1998 Visiting scholar, CSIRO Horticulture Unit, Molecular Plant Pathology Laboratory CSIRO, University of Adelaide, South Australia. Isolated grape berry RNA and screened for PR gene induction in a collaborative effort with Dr. Ian Dry Oct - Dec 1999 Visiting scholar, CSIRO Horticulture Unit, Molecular Plant Pathology Laboratory CSIRO, University of Adelaide, South Australia. Continued work with Dr. Ian Dry on the molecular aspects of ontogenic resistance in grape berries to powdery mildew. Oct 2000 - Jan 2001 Visiting scholar at CSIRO, Molecular Plant Pathology Laboratory, University of Adelaide, Waite Campus, South Australia. Continued collaboration with Drs. Ian Dry and Simon Robinson on induction of PR genes, including a germin-like protein during infection of grape berries by Uncinula necator. Investigated papillum formation and other host responses to infection in susceptible and resistant berries. Further, recorded the effect of the resistance gene run-1 in grape leaves on powdery mildew establishment. 3 Oct - Dec 2001 Visiting scholar at CSIRO, Molecular Plant Pathology Laboratory, University of Adelaide, Waite Campus, South Australia. Continued collaboration with Drs. Ian Dry and Simon Robinson on morphological and physiological responses of Vitis vinifera berries to U. necator. TEACHING 1997 Magical Mushrooms, Mischievous molds (Teaching assistant to Dr. George Hudler) 2003 - 2004 Molecular Plant Ecology Practical course (Teaching assistant to Dr. Jos Raaijmakers) PROFESSIONAL SOCIETIES AND WORKING GROUPS American Phytopathological Society International Working Group on Grapevine Powdery and Downy Mildew Modeling International Organization for Biological Control RESEARCH GRANTS 1999 - 2002 Gadoury, D.M., Seem, R.C., Wilcox, W.F., Goffinet, and Ficke, A. Development of Practical Models for use in the Management of Grape Powdery Mildew: Resistance to Powdery Mildew in Grape Berries. USDA Viticulture Consortium Competitive Grants Program. 1998 - 2001 Ficke, A. Gadoury, D.M., Seem, R.C., Wilcox, W.F., and Goffinet, M. Anatomical, morphological, and genetic basis of ontogenic resistance to powdery mildew in grapevine. Kaplan Research Foundation Competitive Grants Program. 2002-2003 Ficke, A., Raaijmakers, J.M. Biosurfactant producing Pseudomonas to control Phytopthora in different crops. DAAD (Deutscher Akademischer Austausch Dienst), competitive foreign exchange program.. 2002-2003 Ficke, A, Raaijmakers, J.M. Responses of oomycete pathogens to metabolites produced by Pseudomonas fluorescens. EPS (Experimental Plant Sciences), competitive post doctoral grant program. 4 PUBLICATIONS Books, Book Chapters, and Proceedings Ficke, A., de Souza, J., de Boer, M., Geerds, C., Raaijmakers, J. 2004. Biosurfactants and biological control of plant pathogens. In: Multitrophic Interactions in Soil and Integrated Control (eds.: Sikora, R., Gowen, S., Hauschild, R., and Kiewnick, S). IOBC wprs BulletinVol. 27 (1), pp.63-66. Gadoury, D.M., Ficke, A., Seem, R.C., Wilcox, W.F. 1999. Ontogenic Resistance to Powdery Mildew (Uncinula necator) in Grape Berries Proc. 1st Int. Conf. Powdery Mildews, 29 Aug-3 Sept. 1999. 92pp. Gadoury, D.M., Seem, R.C., Ficke, A., Wilcox, W.F., and Dry, I.B. 2002. Ontogenic resistance to Uncinula necator in grape berries: Impacts upon the pathogen, disease progress, and management of powdery mildew. pp 52-53 in: Proc. 4th Int. Workshop on Grapevine Downy and Powdery Mildew, Napa, California, 30 Sept. - 4 Oct., 2002. UC Davis Press. 88 pp. Refereed Journal Papers Ficke, A, J. M., Tran Ti Thu, H., Geerds,C., and Raaijmakers, J.M., 2008. Efficacy of controlling various diseases in crop plants by Pseudomonas fluorescens, SS101. In preparation. Tran, H., Ficke, A., Asiimwe, T., Hoefte, M., Raaijmakers, J.M. 2007.Role of the cyclic lipopeptide massetolide A in biological control of Phytopthora infestans and in colonization of tomato plants by Pseudomonas fluorescens. New Phytologist 175:731-742. Gadoury, D.M., Seem, R.C., Wilcox, W.F., Henick-Kling, T., Conterno, L., Day, A., and Ficke, A. 2007. Effects of diffuse colonization of grape berries by Uncinula necator on bunch rots, berry microflora, and juice and wine quality. Phytopathology 97:1356-1365. Ficke, A., Gadoury, D.M., Seem, R.C, Godfrey, D., and Dry, I.B. 2004. Host barriers and responses to Uncinula necator in developing grape berries. Phytopathology 94:438445. Ficke, A. Gadoury, D.M. Seem, R.C., and Dry, I.B. 2003. Effects of ontogenic resistance upon establishment and growth of Uncinula necator on grape berries. Phytopathology 93:556-563. Gadoury, D.M., Seem, R.C., Ficke, A., and Wilcox, W.F. 2003. Ontogenic resistance to powdery mildew in grape berries . Phytopathology 93:547-555. Ficke, A. Gadoury, D.M., and Seem, R.C. 2002. Ontogenic resistance and plant disease management: A case study of grape powdery mildew. Phytopathology 92:671-675. 5 Gadoury, D.M., Seem, R.C., Ficke, A., and Wilcox, W.F. 2001. The epidemiology of powdery mildew on Concord grapes. Phytopathology 91:948-955. Hill.G.T., Mitkowski, N.A., Aldrich-Wolfe, L., Emele, Jurkonie, D.D., Ficke, A. Maldonado-Ramirez, S., Lunch, S. T. and Nelson, E.B. 2000. Methods for assessing the composition and diversity of soil microbial communities. Appl. Soil Ecol. 15:25-36. Published Abstracts of Presentations at Scientific Meetings Ficke, A., Elen, O., Abrahamsen, U., Brodal, G. 2010. Leaf blotch disease complex in Norwegian wheat. Phytopathology 100:S36 Ficke, A., Abrahamsen, U., Rafoss, T. 2010. The effect of small shifts in climate conditions on the epidemiology of leaf blotch diseases in Norwegian wheat. NJF report Vol 6, Nr 1, S:96 Ficke, A., Gadoury, D.M., Seem, R.C., Goffinet, M., and Dry, I.B. 2001. Comparison and contrast of ontogenic resistance to Uncinula necator in Chardonnay and Concord grape berries. Phytopathology 91:S194. Ficke, A., Gadoury, D.M., Seem, R.C., Goffinet, M.C., and Dry, I.B. 2001. Characterization of potential host barriers to Uncinula necator in developing grape berries. Phytopathology 91:S28. Ficke, A., Gadoury, D.M., Seem, R.C., Goffinet, M., and Dry, I. 2000. Anatomical responses in grape berries to infection by Uncinula necator. Phytopathology 90:S24. Gadoury, D.M., Ficke, A., Seem, R.C., Wilcox, W.F., and Dry, I. 2000. Ontogenic resistance to powdery mildew (Uncinula necator) in grape berries. Proc. 5th International Symposium on Cool Climate Viticulture and Oenology. 16-20 January 2000, Melbourne, Australia. Gadoury, D.M., Seem, R.C., Ficke, A. Wilcox, W.F., and Henick-Kling, T. 2000. Diffuse infections of Uncinula necator predispose grape berries to bunch rot and spoilage microorganisms, and degrade wine quality. Phytopathology 90:S26. Ficke, A., Gadoury, D.M., Seem, R.C., and Dry, I.B. 1999. Pathogen development and host response during infection of grape berries by Uncinula necator. Phytopathology 89:S25. Gadoury, D.M., Ficke, A., Seem, R.C., Wilcox, W.F., and Dry. I. 1999. Ontogenic resistance to powdery mildew (Uncinula necator) in grape berries. Proc. 1st Int. Conf. Powdery Mildews. Avignon, France, 29 Aug. - 2 Sept. 1999. 6