Varian 940-LC Liquid Chromatograph Technical
Specifications
Design Overview
The Varian 940-LC analytical to preparative high performance liquid chromatograph is an
integrated, pre-configured, pre-plumbed system that can be controlled by Varian Galaxie™
Chromatography Software on a single computer or through a fully networked client and
acquisition server. The high pressure, reciprocating single piston pumps with pressure
module delivers consistent, reliable solvent flow throughout the HPLC system. The
autosampler direct injection method ensures carryover of less than 0.01%. The Varian 445LC Scale-Up Module provides automatic column switching, allowing change-over from
analytical to preparative operation. When using the preparative HPLC system, a third pump
with a 25 mL pump head is used for sample injection. The UV or diode dual array detector is
provided as standard — both provide high sensitivity and low noise. A fraction collector
provides automatic sample collection.
These specifications represent typical performance.
High Pressure Gradient Solvent Delivery System
High pressure binary gradient pump.
Type
2 x reciprocating single piston, rapid refill design
Maximum pressure
32 MPa (4600 psi, 320 bar) from 0.025 to 25 mL/min
14 MPa (2000 psi, 140 bar) to 50 mL/min 8 MPa (1200
psi, 80 bar) to 100 mL/min
Number of solvents
Maximum of 4
Flow rate range
0.025 to 25 mL/min with 25 mL heads
0.05 to 50 mL/min with 50 mL heads
0.1 to 100 mL/min with 100 mL heads
Flow accuracy
1% of selected flow or 0.05% of maximum flow,
whichever is larger
Flow reproducibility
1% of selected flow or 0.05% of maximum flow,
whichever is larger
Autosampler
Maximum number of samples
Autosampler syringe volume
Injection volume range
Injection repeatability
Carryover
128 x 4.5 mL HPLC vials
200 x 2 mL HPLC vials
3 x 96-well microplates
or 3 x 384 well microtitre plates
5 mL
0.005 to 4.5 mL
0.3% RSD (using a 500 µL injection by a 5 mL syringe)
<0.01%
UV Detector
Light source
Wavelength range
Spectral bandpass
Wavelength accuracy
Wavelength precision
Noise
Drift
Data acquisition rate
Maximum pressure rating
Flow cells
Deuterium
190 nm to 900 nm
6 nm
±1 nm at 260 nm
±0.1 nm
±5x10-6 AU
<1x10-3 AU/h
20 Hz single wavelength mode
Flow cell rated to 7 MPa (1000 psi, 70 bar)
4 x 0.15 mm dual pathlength super prep (standard for
preparative system)
4 x 0.25 mm dual pathlength scale-up (standard for
semi-preparative system)
9 x 1 mm semi-preparative dual pathlength
Diode Array Detector
Light source
Wavelength range
Wavelength accuracy
Spectral resolution
Digital resolution
Noise
Drift
Data acquisition rate
Maximum pressure rating
Flow cells
preparative system)
Deuterium for UV and quartz halogen for visible
190 to 950 nm
±1 nm verified with 486 nm D2 line
<1 nm per diode
1 nm
±10x10-6 AU
<1x10-3 AU/h
10 Hz
Flow cell rated to 7 MPa (1000 psi, 70bar)
4 x 0.15 mm dual pathlength super prep (standard for
4 x 0.25 mm dual pathlength scale-up (standard for
semi-preparative system)
9 x 1 mm semi-preparative dual pathlength
Refractive Index Detector (Optional)
Refractive index range
Noise
Drift
Linearity
Flow cell temperature range
Flow cell dead volume
Maximum Pressure Rating
1.00 to 1.75
5x10-9 RIU
<250x10-9 RIU/h
600x10-6 RIU
30 to 50 °C in 1 °C increments
6 µL
100 kPa (15psi, 1 bar)
Scale-Up Module
High pressure valves
Port size: Accepts 10-32 male-threaded fittings
Flow passage diamters: Stator 0.3 mm and 0.25 mm
for vertical port
Rotor seal: 0.25 mm
Port-to-port volume: 445 nL and 586 nL with vertical
port
Low-pressure valves
Maximum pressure: 41 MPa (6000 psi, 410 bar)
Actuation time: 100 ms
Orifice diameter: 0.81 mm
Internal volume: 45 µL
Ports: 2/5-28 flat bottom
Operating pressure: Vacuum – 70 kPa (10 psi, 0.7 bar)
(normally open/normally closed)
Vacuum – 172 kPa (25 psi, 1.7 bar) (common)
Wetted parts: Perfluoroelastomer and PEEK
Fraction Collector
Maximum sample racks
Typical number of containers
Maximum tube height
Rack options
3
180
150 mm
Type 21 – 21 x 30 mm OD containers
Type 24 – 24 x 25 mm OD containers
Type 40 – 40 x 20 mm OD containers
Type 60 – 60 x 16 mm OD containers
Type 90 – 90 x 13 mm OD containers
Type 128 – 128 x 15 mm OD containers
Dimensions (W x D x H)
Weight
Divert valve
Type 200 – 200 x 12 mm OD containers
400 x 600 x 480 mm
18 kg
Orifice diameter: 0.81 mm
Internal volume: 45 µL
Ports: ¼-28 flat bottom
Operating pressure: Vacuum – 70 kPa (10 psi, 0.7 bar)
(normally open/normally closed)
Vacuum 172 kPa (25 psi, 1.7 bar) (common)
Wetted parts: Perfluoroelastomer and PEEK
Manual Injector
Port size
Flow passage diameters
Rotor seal
Port-to-port volume
Maximum pressure
Actuation time
Accepts 10-32 male-threaded fittings
Stator 0.3 mm and 0.25 mm for vertical port
0.25 mm
445 nL and 586 nL with vertical port
41 MPa (6000 psi, 410 bar)
100 ms
Input/Output
Start out
Ready Out
Start in
Ready in
Dimensions and Weights
Instrument dimensions (W x D x H)
Instrument weight
750 x 725 x 665 mm
No heavier than 137 kg
Recommended Environmental Conditions
Instrument storage
5 to 45 °C at 20 to 80% relative humidity, noncondensing, altitude <3050 m
Instrument operation
10 to 35 °C, 8 to 80% relative humidity, noncondensing, altitude <2000 m
Instrument electrical requirements
Mains supply of 100 to 120 V AC or 220 to 240 V AC,
50 to 60 Hz with 800 VA for semi-preparative LC and 1200 VA for preparative LC power
consumption