Who are the microorganisms Those of us who do not work with or study microscopic organisms have a tendency to clump the living world that is invisible to the naked eye into a large, nondescriptive categories: __, microbes, or even simply germs. Biologists themselves have historically been guilty of tossing these diminutive to into catch-all groups irrespective of their actual relationships. You may remember learning the five-kingdom classification in school. This system split single-celled organisms into two groups - Kingdom Monera for the Bacteria and associates and Kingdom Protista for every other unicellular creature. While this classification overlooked the great diversity of microorganisms, it was actually an improvement over the previous system, one that labeled every living thing as either Animal or Plant! Starting in the 19??’s, researchers used improvements in genetic techniques to piece together a classification system that more accurately reflects evolutionary history and the vast genetic diversity in the microbial world. By focusing on the similarities and differences in the DNA code used by all living things, scientists could build a “family tree” based on true relatedness, rather than on superficial resemblance. The concept is fundamentally simple. The Tree of Life, like any family tree, begins with a common ancestor. Distantly related groups that took on separate lineages long ago branch off near the base of the tree, while closely related groups that have diverged only recently appear close together. To fill in the tree, taxonomists have exploited the fact that all organisms share the DNA molecule as genetic blueprint and that changes to this molecule are what make each species unique. Mutations in the genetic code are passed down only in the lineage in which they occur and thus genetic relatedness gives an accurate representation of evolutionary relatedness. It turns out that certain regions of the genetic code are more useful in assembling the Tree of Life than others. One of the most useful regions encodes instructions for cellular organelles called ribosomes. All living things rely on ribosomes to translate genetic information into functional proteins within cells but their specific sequences vary enough that they can be used to compare even closely related species. The picture that emerged from such “phylogenetic” investigations was radically different from that of traditional classification. No longer did the macroscopic plants and animals dominate as in the five-kingdom system. Instead, the fundamental division of living things became the three domains: Bacteria, Archaea and Eukarya. To the casual observer this system may seem to give disproportional weight to microorganisms, which occupy all of the first two domains and much of Eukarya, as well! But… genetic /physiological/ ecological differences relative sizes