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0469269;!
PREFACE BY THE SECRETARY GENERAL ....................................................... 5
1.
AUTHORS RESPONSIBLE FOR THE CONTENTS .............................................. 6
2.
TERMS OF REFERENCE ...................................................................................... 7
3.
BACKGROUND...................................................................................................... 8
4.
HISTORY .............................................................................................................. 11
5.
SOCIAL ASPECTS............................................................................................... 18
Identifying the Product......................................................................................................... 18
Target Audience................................................................................................................... 20
Interpol Website on the Internet........................................................................................... 22
6.
THE USE OF DNA TECHNIQUES FOR CRIMINAL INVESTIGATION................ 23
Introduction.......................................................................................................................... 23
The Capability of DNA Profiling......................................................................................... 23
DNA in general Crime Scenes ............................................................................................. 24
7.
INTELLIGENCE LED DNA SCREENING............................................................. 25
Confidential
Planning the Operation......................................................................................................... 25
The Crime Stain ................................................................................................................... 26
The Crime ............................................................................................................................ 26
Identifying the Population to be Sampled............................................................................ 27
Example of Prioritization Grid............................................................................................. 30
The Sampling Team ............................................................................................................. 30
The Sampling Kits ............................................................................................................... 31
Sampling .............................................................................................................................. 31
Investigator Check List ........................................................................................................ 31
Frequently Asked Questions ................................................................................................ 32
Submission / Processing Samples ........................................................................................ 33
Feeding Back of Results ...................................................................................................... 33
8.
THE INTERPOL DNA DATABASE AND EXCHANGE OF DNA PROFILES....... 34
How will the System work? ................................................................................................. 34
What DNA Profiles should be submitted?........................................................................... 35
9.
QUALITY ASSURANCE....................................................................................... 36
10.
FUTURE DIRECTIONAL WAY FORWARD ......................................................... 38
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+??267:32;)
1.
WHAT IS DNA PROFILING.................................................................................. 40
Historic Review: RFLP........................................................................................................ 40
Today´s Technology: The PCR Technology ....................................................................... 41
Mitochondrial DNA (mt-DNA) ........................................................................................... 42
The Performance of the Forensic DNA Laboratory............................................................. 42
Population Genetic Issues .................................................................................................... 43
Harmonization of the DNA Technology world-wide .......................................................... 43
DNA Profile ......................................................................................................................... 44
2.
DNA SAMPLING AND EVIDENCE COLLECTION .............................................. 46
Definitions............................................................................................................................ 46
Personal Protection Guidelines ............................................................................................ 47
Disinfection Guidelines........................................................................................................ 47
Specification and Contents of DNA Reference Sampling Kits ........................................... 47
Sexual Offences Medical Examination Kits ........................................................................ 48
Contents of Scene of Crime Stain Sampling Kits ................................................................ 49
General Guidelines for Sampling Different Types of Biological Samples.......................... 51
Anti-Contamination Guidelines ........................................................................................... 51
Conditions for Storing the Samples ..................................................................................... 52
Transport to the Laboratory ................................................................................................. 53
3.
TRAINING............................................................................................................. 54
Scene .................................................................................................................................... 54
First Officers / First Responders .......................................................................................... 54
Specialist Scene of Crime Examiners .................................................................................. 55
Laboratory............................................................................................................................ 57
Legal Courts and the Judicary.............................................................................................. 59
Public, Community and General Awareness ....................................................................... 61
4.
INTERPOL STANDARD SET OF LOCI: ISSOL................................................... 62
5.
INTERPOL DNA PROFILE SEARCH REQUEST FORM..................................... 63
6.
TABLES OF WORLDWIDE MOSTLY USED LOCI.............................................. 65
7.
LIST OF MEMBERS OF THE INTERPOL DNA MEG .......................................... 67
8.
INDEX ................................................................................................................... 68
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!
THE INTERPOL STANDARD SET OF LOCI - I S S O L
Loci
Example
VWA
TH01
D21S11
FGA
D8S1179
D3S1358
D18S51
15
3
8
5
12
15
13
20
6
9.3
5
13
R
15
X
Y
The minimum input to
the Interpol DNA ASF
database is 6 STRs
R = rare allele which is not
mentioned in the list of alleles
accepted in the national DNA
database(s)
Option
Amelogenin
H! T2H! %YG%&$;! 'GG)(#$%9! A:! $D%! 2+Z! -&%$)&('! '#9! &%G&%8%#$(#0!
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T2H! %YG%&$8! $)! `)(#! $D%! "#$%&G)*! T2H! O4L! =#$(*! $D%! .=&&%#$!
,%,A%&8!.',%!$)!$D%!%#9!)M!$D%(&!$%&,8!)M!)MM(.%!(#!$D%!CG&(#0!)M!
>??>F!
>2T!"23452H3"/2H6!T2H!
PC45Cf!+/274542+4!
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M)&! "#I%8$(0'$(I%! /MM(.%&8;! 6:)#;! V! $)! W! 2)I%,A%&! >??@! E%&%! '*8)!
9(8.=88%9F!3D%!'0%#9'!E(**!.)#$'(#!$E)!,'(#!$)G(.8_!
#4?:3)!)N),"+),898O8;2;)
3D%! G&)0&',,%! E(**! #)$! M).=8! )#! $D%! A'8(.! 8.(%#.%! )M! T2H! A=$!
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$&'MM(.N(#0!)M!A)9:!G'&$8;!,'88!9(8'8$%&8!'#9!)$D%&!.'8%8!)M!
#)$%!
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MI) !"#$%&'()G#+",+%,)G$#)'E)('0!_)!GG'()
THE INTERPOL STANDARD SET OF LOCI - I S S O L
Loci
VWA
TH01
D21S11
FGA
D8S1179
D3S1358
D18S51
Example
15
3
8
5
12
15
13
20
6
9.3
5
13
R
15
X
Y
Option
Amelogenin
!
!
]>!
The minimum input to
the Interpol DNA ASF
database is 6 STRs
R = rare allele which is not
mentioned in the list of alleles
accepted in the national DNA
database(s)
!
!
PI) !"#$%&'(),"+)&%'E!($)G$+%0*)%$VJ$G#)E'%A)
!
"!#!$!%!&!'!(!)!!!!!!*!#!+!!!!!!'!&!(!,!"!)!%!!!!!!-!%!+!&!.!/!!!!!!&!%!0!1!%!-!$!!
!
&!!%!!0!!1!!%!!-!!$ !
NCB:
REF:
DATE:
NATIONAL OFFICE REQUESTING SEARCH:
E-MAIL ADDRESS / PHONE / FAX NUMBER:
REF:
TO NCB:
INFO NCB:
(,,%#.% !
CATEGORY:
PLACE:
ADDITIONAL INFORMATION:
*#+!!'&(,")%
DATE:
SUSPECT
C0NVICTED
CRIME STAIN
OTHERS
VWA
THO1
D21S11
FGA
D8S1179
D3S1358
D18S51
Amelogenin
ISSOL ➀
TPOX
CSF1P0
D13S317
D7S820
D5S818
D16S539
D2S1338
D19S433
other
loci
Penta D
Penta E
FES
F13A1
F13B
SE33
CD4
GABA
other
loci
IN CASE OF A NEGATIVE SEARCH PLEASE REPEAT THE SEARCH OF THE PROFILE IN YOUR DATABASE
MONTHLY
QUARTERLY
ANNUALLY
NO
!
&!!%!!'!!)!!2 !
BY NCB:
TO NCB:
INFO NCB:
REF:
DATE:
,())(3"#4!!&%-1)$!!/+-!!5%%#!!(5$+"#%*!!+,$%&!!-%+&./
Disclaimer: No responsibility can be taken for the accuracy or quality of the information provided
NEGATIVE SEARCH
YES
PROFILE MATCH(ES) MADE
SUSPECT
YES
HOW MANY
CONVICTED
CRIME STAIN
PROFILE ADDED TO SEARCHED DATABASE:
PROFILE W ILL BE SEARCHED
NO
OTHERS
YES
MONTHLY
NO
QUARTERLY
ANNUALLY
,())(3"#4!!6+$./7%-8!!,(1#*!!"#!!*+$+5+-%!!-%+&./%*!!➁
Match No.
➁
NCB REFERENCE:
SAMPLE REFERENCE:
VWA
THO1
D21S11
FGA
D8S1179
D3S1358
D18S51
Amelogenin
ISSOL ➀
TPOX
CSF1P0
D13S317
D7S820
D5S818
D16S539
D2S1338
D19S433
other
loci
Penta D
Penta E
FES
F13A1
F13B
SE33
CD4
GABA
other
loci
ADDITIONAL INFORMATION:
!
➀
!
!
➀!!!"##$%!&!'()!"*+),-./!#+0*10,1!#)+!$2!%.34!
➁!!!"*!305)!.2!0114+4.*0/!60+3()5!75)!*768),)1!3.-4)5!.2!+()!,)-/9:-0,+!.2!+(45!2.,6;!
!
]d!
!
LP"T46"24C!7/5!+/O-643"2L!3S4!"2345-/6
T2H!-5/7"64!C4H5+S!54jP4C3!7/5O
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"!#!$!%!&!'!(!)!!!!!!*!#!+!!!!!!'!&!(!,!"!)!%!!!!!!-!%!+!&!.!/!!!!!!&!%!0!1!%!-!$
@b!2',%!)M!$D%!&%R=%8$(#0
!!!!2'$()#'*!!+%#$&'*
!!!!Z=&%'=!)M!"#$%&G)*F
&!!%!!0!!1!!%!!-!!$
@
NCB:
REF:
DATE:
NATIONAL OFFICE REQUESTING SEARCH:
E-MAIL ADDRESS / PHONE / FAX NUMBER:
REF:
TO NCB:
INFO NCB:
>b!+'$%0)&:!)M!$D%
!!!!)MM%#.%F!P8%!,'(#
!!!!D%'9(#08!)#*:g!(F%F
!!!!,=&9%&;!&'G%;!%$.F
db!-*'.%!ED%&%!$D%!.&(,%
!!!!D'8!A%%#!.),,($$%9!)&
!!!!9%$%.$%9!)&!G*'.%
!!!!ED%&%!$D%!.&(,%!8$'(#
!!!!D'8!A%%#!$'N%#F
hb!T'$%!ED%#!$D%!.&(,%
!!!!D'8!A%%#!.),($$%9!)&
!!!!9%$%.$%9F
(,,%#.%
>
d
CATEGORY:
PLACE:
ADDITIONAL INFORMATION:
*#+!!'&(,")%
h
DATE:
X
SUSPECT
C0NVICTED
CRIME STAIN
OTHERS
Amelogenin
ISSOL ➀
D2S1338
D19S433
other
loci
CD4
GABA
other
loci
VWA
THO1
D21S11
FGA
D8S1179
D3S1358
D18S51
TPOX
CSF1P0
D13S317
D7S820
D5S818
D16S539
Penta D
Penta E
FES
F13A1
F13B
SE33
e
IN CASE OF A NEGATIVE SEARCH PLEASE REPEAT THE SEARCH OF THE PROFILE IN YOUR DATABASE
MONTHLY
QUARTERLY
ANNUALLY
NO
&!!%!!'!!)!!2
BY NCB:
TO NCB:
INFO NCB:
REF:
DATE:
,())(3"#4!!&%-1)$!!/+-!!5%%#!!(5$+"#%*!!+,$%&!!-%+&./
∆ΙΣΧΛΑΙΜΕΡ: ΙΝΤΕΡΠΟΛ ΑΧΧΕΠΤΣ ΝΟ ΡΕΣΠΟΝΣΙΒΙΛΙΤΨΦΟΡ ΤΗΕ ΑΧΧΥΡΑΧΨΟΡ ΘΥΑΛΙΤΨΟΦΤΗΕ ΙΝΦΟΡΜΑΤΙΟΝ ΠΡΟς Ι∆Ε∆ ΙΝ ΤΗΙΣ ΡΕΠΛΨ
NEGATIVE SEARCH
YES
PROFILE MATCH(ES) MADE
YES
SUSPECT
CONVICTED
PROFILE ADDED TO SEARCHED DATABASE:
PROFILE WILL BE SEARCHED
NO
HOW MANY
CRIME STAIN
YES
MONTHLY
OTHERS
NO
QUARTERLY
ANNUALLY
,())(3"#4!!6+$./7%-8!!,(1#*!!"#!!*+$+5+-%!!-%+&./%*!!➁
eb!3D%!T2H!G&)M(*%a8b
!!!!M)&!%Y.D'#0%!8D)=*9
!!!!D'I%!A%%#!9%I%*)G%9
!!!!(#!'..)&9'#.%!E($D!'#
!!!!%8$'A*(8D%9!R='*($:
!!!!'88=&'#.%!G&)0&',F
]
➁
Match No.
NCB REFERENCE:
V
V
SAMPLE REFERENCE:
VWA
THO1
D21S11
FGA
D8S1179
D3S1358
D18S51
Amelogenin
ISSOL ➀
TPOX
CSF1P0
D13S317
D7S820
D5S818
D16S539
D2S1338
D19S433
other
loci
Penta D
Penta E
FES
F13A1
F13B
SE33
CD4
GABA
other
loci
X
ADDITIONAL INFORMATION:
➀!"##$%!&!'()!"*+),-./!#+0*10,1!#)+!$2!%.34!!!!!!!!!!!!!!!!!!!!!!➁ "*!305)!.2!0114+4.*0/!60+3()5!75)!*768),)1!3.-4)5!.2!+()!,)-/9:-0,+!.2!+(45!2.,6;
]b!"#!.'8%!)M!'99($()#'*
!!!!,'$.D%8!#=,A%&%9
!!!!.)G(%8!)M!$D%!&%G*:Q
!!!!G'&$!)M!$D(8!M)&,
!!!!8D)=*9!A%!=8%9F
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t
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f
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g
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f
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