BME 128
Thursday April 14
(1) Chapter 9 (Protein Purification)
Half a Quiz
(2) Lecture: Fibrous Proteins
Chapter 4
(3) Twirling around in 3-D: Hemoglobin
A Classic Case Example: Sickle Cell Anemia
- pg 441-442
• eCommons site still in the works (sorry!): HW1 email OK
• Reading/Study Summary Weeks 1-3:
Protein Basics/Structure: Chp 1+2 + Slides + Chp3 (near-completed)
Protein Production/Purification/PTM: Slides + PTMs in Book + Chp9
EXAM WILL FOCUS ON PROTEINS AND METHODOLOGY
Slide Credit: Bchm3100
S.Mosimann (modified)
6"7*#1(,:*#)&"9( ,;*&,%"<%3+,&3#9<;)&4,
:#3+:&:)"4&(,$#':#(&4,#=,;,("9<3&,(&$#94;*+,
()*1$)1*&,&3&'&9)
8*"';*+,$#':#9&9),#=,(>"9?,)&94#9?,7#9&?,
$#99&$)"@&,)"((1&(?, !"#
619$)"#9,;(, ()*1$)1*;3,';)&*";3 ,)%;),%;@&,
:*#)&$)"@&?,$#99&$)"@&,#*,(1::#*)"@&,*#3&(
2"':3"$")+,#=,()*1$)1*&,';>&(,*&3;)"#9,7&)A&&9,
()*1$)1*&,;94,=19$)"#9,*&3;)"@&3+,#7@"#1(
B;*&3+,$*+();33"C&,D;3(#,4"=="$13),=#*,EFBG,(#,()*1$)1*;3,
"9=#*';)"#9,;,@;*"&)+,#=,"94"*&$),)&$%9"H1&(,
0&$)1*&,..
!"#$%&'"()*+,-.// 23"4&,5

F&$%;9"$;33+,41*;73&,;94,19*&;$)"@&,:*#)&"9,#=,@&*)&7*;)&(
1:,)#,JKL,#=,:*#)&"9,"9,%#*9(?,%;"*?,9;"3(,M,=&;)%&*(
α N>&*;)"9(,#$$1*,"9,';'';3(O, β N>&*;)"9(,#$$1*,"9,7"*4(,;94,*&:)"3&(
P,-/,>&*;)"9,<&9&(,&Q:*&((&4,"9,';'';3(
α N>&*;)"9(,;*&,$3;(("="&4,;(,*&3;)"@&3+,;$"4"$,DR+:&,SG,#*,7;("$,DR+:&,SSG,
I&*;)"9(,%;@&,$#':3&Q,H1;)&*9;*+,()*1$)1*&(
D;G,>&*;)"9(,;*&,4"'&*(,$#':#(&4,#=,;,R+:&,S,;94,R+:&,SS,(1719")
D7G,';9+,4"'&*(,;((#$";)&,)#,=#*',:*#)#="3;'&9)(
D$G,:*#)#="3;'&9)(,4"'&*"C&,)#,=#*',:*#)#="7*"3(
D4G,:*#)#="7*"3(,=#*',)&)*;'&*(,$;33&4,'"$*#="7*"3(
D&G,'"$*#="7*"3(,;((#$";)&,"9)#,';$*#="7*"3(
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// 23"4&,-
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,T

NI&*;)"9,2)*1$)1*&
VN*;+,($;))&*"9<,;94,4"==*;$)"#9,()14"&(,"94"$;)&, α N
>&*;)"9,%;(,;,%&3"$;3,()*1$)1*&,A")%,;,KW.,X,:")$%,
D4"();9$&,:&*,)1*9,#=,%&3"QG,
Y;*"&)+,#=,&Q:&*"'&9);3,&@"4&9$&,"94"$;)&, α N>&*;)"9,"(,;,
4"'&*,$#':#(&4,#=,#9&,R+:&,S,;94,#9&,R+:&,SS,
(1719")
Z;$%,(1719"),"(,;,*"<%)N%;94&4, α N%&3"$&(O,)%&,)A#,
%&3"$&(,A*;:,;*#194,#9&,;9#)%&*,)#,=#*',;,3&=)N
%;94&4?,:;*;33&3, $#"3&4N$#"3
R%&,)A"(),#=,)%&,"94"@"41;3, α N%&3"$&(,"9,)%&,$#"3&4N$#"3,
"(,*&(:#9("73&,=#*,)%&,(%#*),%&3"$;3,(:;$"9<,
D9#*';33+,KWT,XG
(for a "proper" & straight alpha-helix)
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,K
α N>&*;)"9,$#9);"9(,;,],*&("41&,:(&14#*&:&;),D $%&%#%'%!%(%) G,A")%"9,)%&,
$&9)*;3,^-./,(&<'&9),#=,)%&,'#3&$13&
B&("41&(,;),:#(")"#9,;,;94,4,;*&,%+4*#:%#7"$,;94,;*&,*&(:#9("73&,=#*,)%&,
;((#$";)"#9,#=,)%&,)A#,(1719")(
R%&,KW.,X,(:;$"9<,#=,)%&, α N%&3"$&(,#:)"'"C&,)%&,;((#$";)"#9,#=,)%&,%+4*#:%#7"$,
*&("41&( residue "i+3"
0&$)1*&,..
residue "i"
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,[

α N>&*;)"9,"(,*"$%,"9,\+(,*&("41&(,;94,4"(13="4&,7*"4<&(
_9&,#=,)%&,4"==&*&9$&,7&)A&&9,)%&,';9+, α N>&*;)"9,:*#)&"9(,"(,)%&"*,\+(,$#9)&9)
R%&,'&$%;9"$;3,()*&9<)%,#=,%#*9(?,9;"3(,;94,(>"9,4&:&94(,1:#9,)%&,$#9)&9),#=,
\+(,*&("41&(,;94,)%&,91'7&*,#=,4"(13="4&,7*"4<&(
R%&,'#*&,4"(13="4&,7*"4<&(,)%&,'#*&,*"<"4,)%&,*&(13)"9<,()*1$)1*&
`"(13="4&(,#$$1*,7&)A&&9,4"'&*(,;94,A")%,#)%&*,:*#)&"9(,)%;),;*&,:;*),#=,
'"$*#="7*"3(,;94,';$*#="7*"3(
ZQ)&9("@&,4"(13="4&,7*"4<"9<,;$$#19)(,=#*,)%&,"9(#317"3")+,;94,*&("();9$&,)#,
()*&)$%"9<,#=, α N>&*;)"9(
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,]
!"#$%&'"()*+,#=,)%&,bc;"*,8&*'d
.G,F&*$;:);9(,;*&,*&41$"9<,;<&9)(,)%;),7*&;>,)%&,9;)1*;33+,#$$1**"9<,
4"(13="4&,7*"4<&(,#$$1*"9<,A")%"9,)%&, α N>&*;)"9,#=,%;"*
D;94,;3(#,<"@&,*"(&,)#,)%&,b(:&$";3d,('&33G
5G,F#"(),%&;),;94,$1*3&*(,;*&,1(&4,)#,'&$%;9"$;33+,$%;9<&,)%&,(%;:&,
D()*&)$%G,#=,)%&,%;"*
;$)1;33+,$;1(&(,;,%&3"Q,)#,$#"3,)*;9(")"#9,;3#9<,:;*)(,#=,)%&, α N>&*;)"9,
-G,_Q"4"C"9<,;<&9)(,;*&,1(&4,)#,*&=#*',)%&,4"(13="4&,7*"4<&(,A%"3&,)%&,
%;"*,"(,%&34,"9,)%&,$%;9<&4,(%;:&
E&A,%;"*,<*#A)%,A"33,()"33,:*#41$&,)%&,9;)1*;33+,#$$1**"9<,4"(13="4&,
7*"4<&(,;94,)%&,b$%;9<&4,(%;:&d,A"33,7&,3#(),;(,)%&,%;"*,<*#A(
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,J

2&@&*;3,"9%&*")&4,4"(&;(&(,*&(13),=*#','1);)"#9(,"9,>&*;)"9,<&9&(
Z!2,D&:"4&*'#3+("(,7133#(;,("':3&QG,;94,Zc2,D&:"4&*'#3+)"$,%+:&*>&*;)#("(G,
;*&,$%;*;$)&*"C&4,7+,(>"9,73"()&*"9<
B;9<&,#=,&==&$)(,=*#',7;*&3+,9#)"$&;73&,)#,"9$;:;$");)"9<,D4&:&94"9<,1:#9,'1);)"#9G
Z!2,*&(13)(,=*#','1);)"#9,"9,&")%&*,>&*;)"9,K,#*,.T,
Zc2,*&(13),=*#','1);)"#9,"9,&")%&*,>&*;)"9,.,#*,./
F1);)"#9,;==&$)(,:*#)#="3;'&9),;94f#*,:*#)#="7*"3,=#*';)"#9 g7&**;9),:*#)#="3;'&9),#*,:*#)#="7*"3,=#*';)"#9,*&(13)(,"9,;,3#((,#=,'&$%;9"$;3,
"9)&<*")+,#=,7;(;3,(>"9,$&33(
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,e
8*&(&9),"9,;33,'13)"$&3313;*,#*<;9"('(,;94,)%&,'#(),;7194;9),:*#)&"9,"9,
@&*)&7*;)&(
&Q)*;$&3313;*,:*#)&"9,)%;),=#*'(,"9(#3173&,="7&*(,#=,<*&;),)&9("3&,()*&9<)%
';h#*,()*&((,7&;*"9<,$#':#9&9),#=,$#99&$)"@&,)"((1&,i,7#9&?,)&&)%?,$;*)"3;<&?,
)&94#9?,(>"9?, !"#*
F;'';3(,%;@&,;),3&;(),--,4"()"9$),$#33;<&9,(1719")(,)%;),=#*',P5/,4"==&*&9),
H1;)&*9;*+,()*1$)1*&(
0&$)1*&,..
!"#$%&'"()*+,-.// S. Mosimann 23"4&,./

\#33;<&9(,%;@&,;,4"()"9$)"@&,;'"9#,;$"4,$#':#(")"#9
9&;*3+,.f-,#=,)%&,*&("41&(,;*&,j3+
;9#)%&*,.KN-/,L,#=,)%&,*&("41&(,;*&,&")%&*,8*#,#*,;,:#()N)*;9(3;)"#9;3,
'#4"="$;)"#9,#=,8*#,DTN%+4*#Q+:*#3"9&G
(';33&*,;'#19),#=,)%&,'#4"="&4,;'"9#,;$"4(?,-N%+4*#Q+:*#3"9&,;94,KN
%+4*#Q+3+("9&,;3(#,#$$1*,"9,$#33;<&9
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,..
8*#3+3,%+4*#Q+3;(&,"(,)%&,&9C+'&,)%;),$#9@&*)(,:*#3"9&,)#,TN%+4*#Q+:*#3"9&
*&H1"*&(,;($#*7"$,;$"4,D@");'"9,\G,=#*,;$)"@")+O,3;$>,#=,4"&);*+,@");'"9,\,3&;4(,)#,
($1*@+ ,;(,TN%+4*#Q+:*#3"9&,$;99#),7&,(+9)%&("C&4
;7(&9$&,#=,TN%+4*#Q+:*#3"9&,:*&@&9),:*#:&*,$#33;<&9,="7&*,=#*';)"#9
2$1*@+,"(,$%;*;$)&*"C&4,7+,(>"9,3&("#9(?,73##4,@&((&3,=*;<"3")+?,:##*,A#194,
%&;3"9<,;94,"(,13)"';)&3+,=;);3
,
TN%+4*#Q+:*#3"9&,"(,*&(:#9("73&,=#*,();7"3"C"9<,$#33;<&9,()*1$)1*&
$#33;<&9,(+9)%&("C&4,A")%#1),TN%+4*#Q+:*#3"9&,4&9;)1*&(,;),5Tk,\?,A%&*&;(,
9#*';3,$#33;<&9,4&9;)1*&(,;),-ek,\ ,
;::;*&9)3+?,();7"3"C"9<,"(,@";,%+4*#<&9,7#94(,)#,l7*"4<"9<l,A;)&*,'#3&$13&(,
3#$;)&4,7&)A&&9,"94"@"41;3,(1719")(,#=,)%&,$#33;<&9,="7&*
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,.5

\#33;<&9,"(,$#':#(&4,#=,;,*&:&;)"9<,j3+,i,V,i,m,(&H1&9$& ,
)%&,V,:#(")"#9,"(,)+:"$;33+,8*#,;94,)%&,m,:#(")"#9,"(,#=)&9,
%+4*#Q+:*#3"9&
;33#A(,)%*&&,$#33;<&9,:#3+:&:)"4&(,)#,A*;:,;*#194,#9&,;9#)%&*,
=#*'"9<,;,*"<%)N%;94&4,)*":3&,%&3"Q
&;$%,:#3+:&:)"4&,"(,%&3"$;3,A")%,9n-W-,;94,;,:")$%,#=,./W/,X j3+,*&H1"*&4,;),&@&*+,-
:;$>"9<,#=,(1719")(
*4 ,
:#(")"#9,)#,;33#A,$3#(&,
;9+,#)%&*,*&("41&,4"(*1:)(,
)*":3&,%&3"Q
&Q)*&'&,)&9("3&,()*&9<)%,41&,
)#,$3#(&,:;$>"9<,
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,.-
0&$)1*&,..
R%*&&,:#3+:&:)"4&(,#=,$#33;<&9,
%&3"Q,;*&,();<<&*&4,*&3;)"@&,
)#,#9&,;9#)%&*
:3;$&(,j3+,;),)%&,$&9)&*,#=,)%&,
)*":3&,%&3"Q,;),&@&*+,:#(")"#9,
;3#9<,)%&,$#33;<&9,="7&*
:#3+:&:)"4&,%&3"$&(,;*&,%"<%3+,
&Q)&94&4,;94,%;@&,;,3&=)N
%;94&4,)A"(),A%"3&,)%&,)*":3&,
%&3"Q,"(,*"<%)N%;94&4 j3+,;),&@&*+,)%"*4,:#(")"#9,
$#9)*"71)&(,)#,)&9("3&,
()*&9<)%,#=,%&3"Q
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,.T

2&@&*;3,$#''#9,$#33;<&9(,=#*',4"()"9$)"@&3+,7;94&4,="7*"3(
R+:&,S,$#33;<&9(,%;@&,;,4";'&)&*,#=,.//N5///,X,;94,;,:&*"#4"$")+,#=,[J/,X
!;94"9<,;*"(&(,=*#',:;$>"9<,#=,$#33;<&9,'#3&$13&(,"9,)%&,="7*"3
%+4*#:%#7"$,"9)&*;$)"#9(,;*&,)%&,4*"@"9<,=#*$&,=#*,;((#$";)"#9,#=,$#33;<&9,)*":3&,
%&3"$&(,"9)#,;,="7*"3
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,.K
2)*&9<)%,;94,"9(#317"3")+,#=,
$#33;<&9,="7*"3(,"(,;3(#,41&,
)#,"9)*;N,;94,"9)&*'#3&$13;*,
$#@;3&9),$*#((N3"9>(
E#),4"(13="4&,7#94(,;(,
$#33;<&9,"(,9&;*3+,4&@#"4,
#=,\+(
\*#((N3"9>(,;*&,7&)A&&9,0+(,
D;94,c"(G,*&("41&(
1:,)#,T,*&("41&(,$;9,7&,
"9@#3@&4,"9,;,("9<3&,$*#((N,
3"9>&4
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,.[

+$",-./012'2.$"/0 ,"(,;,(A&&),:&;,)%;),$#9);"9(,("<9"="$;9),;'#19)(,
#=, β N;'"9#:*#:"#9")*"3&
β N;'"9#:*#:"#9")*"3&,"(,;9,"9%"7")#*,#=,3+(+3,#Q"4;(&,;94,:*&@&9)(,)%&,
$*#((,3"9>"9<,#=,$#33;<&9,="7*"3(
S9$*&;(&4,=*;<"3")+,#=,$#33;<&9,="7*"3(,3&;4(,)#,;79#*';3")"&(,#=,7#9&(?, h#"9)(,;94,3;*<&,73##4,@&((&3( g<"9<,"9$*&;(&(,)%&,$*#((N3"9>"9<,#=,$#33;<&9
9#),$&9)*;3,$;1(&,#=,;<"9<,;(,3;)%+*"(',%;(,9#,&==&$),#9,3"=&(:;9
&Q:3;"9(,A%+,'&;),=*#',#34&*,;9"';3(,"(,)#1<%&*,;94,A%+,#34&*,
"94"@"41;3(,%;@&,3&((,=3&Q"7"3")+
0&$)1*&,..
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 23"4&,.]
\#33;<&9,4&=&$)(,;94,
4"(&;(&
2&@&*;3,*;*&,%&*");73&,4"(#*4&*(,#=,$#33;<&9,;*&,>9#A9
7*"))3&,7#9&,4"(&;(&,$;9,;*"(&,=*#',;,("9<3&,:#"9),'1);)"#9,"9,$#33;<&9,#=,
7#9&
%+:&*&Q)&9("7"3")+,#=,h#"9)(,;*"(&(,=*#',3&((&9"9<,#=,$*#((,3"9>"9<,"9,$#33;<&9,
#=,3"<;'&9)(
#()&#;*)%*")"(,;94,;)%&*#($3&*#)"$,:3;H1&(,;*"(&,=*#',4"(*1:)"#9,#=,$#33;<&9,
"9,$;*)"3;<&
Z;$%,4"(&;(&,*&(13)(,"9,)%&,4"(*1:)"#9,#=,)%&,)*":3&,%&3"$;3,()*1$)1*&,#=,;,
(:&$"="$,$#33;<&9
Slide Credit: !"#$%&'"()*+,-.// S. Mosimann 0&$)1*&,..
23"4&,.J

Homework Turn-In & Late Policy: Homeworks are due at the specified time. Homeworks turned in late have 5% deducted if turned in within 24 hours, and an additional 10% for every additional day late until homeworks are returned or answers are given in class or on-line (generally one week after homeworks are turned in). You cannot get credit at all if you submit your homework later than that - therefore you have to contact us prior to the due date/time if you have a serious circumstance!
Academic Honesty and Integrity:
What is Academic Integrity? This question is better answered with how we violate academic integrity. One prime example is fabrication.
Fabrication: In any academic exercise, submitting falsified data including bibliographic resources and experimental data, or altering graded coursework/exams and resubmitting to the instructor for a higher score.
Facilitating Academic Dishonesty is a violation, too: One form of this is answering questions on someone else's exam or doing someone else's homework for them.
Another form is helping another student take a test
(allowing them to cheat from you).
See our official University Policy on Academic Integrity for Undergraduate Students
Figure Credit: http://sickle.bwh.harvard.edu/malaria_sickle.html

… Can we understand by looking at the structure?