Sabancı University
Nanotechnology Research and Application Center
(SUNUM)
LABORATORY SAFETY SEMINAR
Introduction
Part 1. SUNUM Facilities
Part 2. Emergency Procedures
Part 3. Hazards & Safety
Architecture
 Architecture inspired by
human cell
Architecture
BASEMENT
Half basement added later
 7.400 m2 research
building
 2 full floors and half
basement
Layout: Lab floor
Layout: Lab floor
Vibration
isolation
Layout: Lab floor
Dedicated Laboratories
• Micro/Nano Fabrication Lab (Class 100- 1000 clean room)
• Molecular Biology (Genome sequencing capability) (BSL2)
• Electron Microscopy and Spectroscopy
• Material Growth
• Material Characterization
• Nanoelectronics
• Nanomagnetics
• Energy Systems
• Micro/Nano-fluidics
• Micro-Nano Packaging
• Advanced Microscopy
• Testing & Characterization (up tp 60 GHz)(Anechoic
chamber)
• 3D Micro/Nano Prototyping
• Tissue and Regenerative Engineering (BSL2)
• Precision Manufacturing
Green Building

Conforming to international ‘Green’
standards: LEED - Gold Level (USA) and
BREEAM (English) certified

Built on a parking lot to be ecologically biocompatible with the surroundings

Endemic, low water consuming plants selected
for landscape: 72 trees and bushes, 5749
plants, 7380 ground-covers replacing and
adding to 795 transplanted species

Recovery and reuse of rainwater

Large area thermally isolating windows and
glass roof for natural lighting

Minimal water usage in the building by
atomated turn-off, low debit fixtures
8
Supplied Services
 ASTM Type 1 and Type 2 DI water
(8 and 18 Mohm loops)
 5 different gases centrally
supplied using high purity lines
 Aircuity environment control for
hazardous conditions
 Common neutralization system for
laboratory water, acid and alkaline
discharge
9
Gases, Vacuum and Compressed Air
 5 different gases distributed to all labs from an enclosed location placed
outside of the buiding under one of the stairs
 One wall of the enclosure weakened to absorb the schock of explosions
 Services provided: Ar, High Purity N2, H2, Spare,
 Liquid nitrogen Tank (3T) outside of the building for common N2
 Vacuum and dry-generated compressed air services in the basement
10
LAB SAFETY
•
•
•
•
•
•
•
General Principles
Emergency Procedure
Chemical Hygine Plan
Storage
Chemical Spill
Hazardous Waste Disposal
First Aid
11
General Safety Guidlines
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
NEVER perform any hazardous work alone in the laboratory. At least two people should be present.
NEVER perform unauthorized work, preparations or experiments.
ALWAYS wear proper eye protection in chemical work, handling and storage areas. Contact lenses should normally not be worn.
Prepare and maintain a chemical inventory for the lab.
Consult material safety data sheets (MSDS) before working with hazardous chemicals or infectious material.
ALWAYS know the hazards associated with the materials that are being utilized in the lab.
ALWAYS wear appropriate protective clothing. Cover shorts, cut-offs or miniskirts with a suitable lab coat or apron.
Confine long hair and loose clothing. Do not wear high-heeled shoes, open-toed shoes, sandals or shoes made of woven material.
ALWAYS wash hands and arms with soap and water before leaving the work area.
NEVER remove chemicals, biological agents, or radioactive materials from the facility without proper authorization.
Be familiar with the location of emergency equipment - fire alarm, fire extinguisher, eye wash stations and safety showers.
Know the appropriate emergency response procedures
Use equipment and hazardous materials only for their intended purposes.
NEVER mouth pipette chemicals when transferring solutions. Instead, you should always use a pipette bulb to transfer
solutions.
Use a hood whenever there is a possibility of poisonous or irritating fumes being emitted.
NEVER leave an experiment unattended while it is being heated or is rapidly reacting.
Keep equipment back from the edge of the lab bench to prevent spillage.
Support all beakers and flasks with clamps. Do not use cracked or chipped glassware.
NEVER engage in horseplay, pranks or other acts of mischief in chemical or biological work areas
Report accidents and dangerous incidents ("near-misses") promptly to your supervisor and SUNUM Facilities Management.
Inform your supervisor and SUNUM Facilities Management if you realize that any equipment is broken, not working properly or
not calibrated.
12
Emergency Procedures
13
Emergency Procedures
Question: What should
you do in an emergency?
• Fire?
• Accidents?
• Other emergencies?
Answer:
Call 5555 or 6666
from any campus phone
Emergency Procedures
 Fire Emergency
1. NOTIFY people in the immediate area that
there is a fire
2. ACTIVATE the nearest FIRE ALARM
3. Locate the nearest campus telephone away
from the fire area and DIAL 5555
4. FIGHT THE FIRE IF YOU CAN (see side panel)
5. EVACUATE the area by using the stairwells.
6. NEVER re-enter the building until told by
Officials
15
Emergency Procedures
 Earthquake Response
1. “DROP, COVER AND HOLD ON.”
2. AVOID windows, hanging
objects, mirrors, tall furniture
3. DO NOT TRY TO RUN OUT OF
BUILDING.
4. Do not use elevators.
5. EVACUATE the area by using the
stairwells.
6. NEVER re-enter the lab until told
by Officials.
16
Emergency Procedures
 Hazardous Material Spill/Gas Leak
1. ALERT people in the laboratory to
evacuate.
2.ACTIVATE the Emergency Button
next to lab door.
3.CALL SUNUM Facility Management
2424 or 5555 any time.
4. ISOLATE the affected area.
5.NEVER re-enter the lab until told
by Officials
17
Emergency Evacuation Plan
18
LAB EMERGENCY POSTER
Call 5555
Medical Emergency
Dial 5555 or 6666
LIFE THREATENING EMERGENCY:Dial 5555 or contact the University Medical Center Emergency directly at 6666 (located at D2 building-2
Floor). Note: All injuries must be reported to SUNUM Facilities Management within 8 hours.
NON-LIFE THREATENING EMERGENCY: Go to University Medical Center.
Needle stick/puncture exposure: Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Note: All needle stick/puncture exposures must be reported to
SUNUM Facilities Management within 8 hours.
Fire
Dial 5555
SMALL FIRE (trash can size): If you have been trained, you may put out the fire using a fire extinguisher. Report the fire by calling 5555. Notify
Security 2440 and SUNUM Facilities Management at 2424 at anytime a fire extinguisher is used or discharged.
LARGE FIRE (requiring more than 1 fire extinguisher): Evacuate the area. Close all doors and windows as you leave. Close the fume hood
sash if the fire is in the fume hood. Activate the nearest alarm. Call 5555. Evacuate the area using the stairwells. Do not use the elevator.
CLOTHES ON FIRE: Use nearest safety shower. If none immediately available, STOP-DROP-ROLL to quickly smother the fire. Seek medical
attention. Notify Security and Health Center at 5555 immediately.
Chemical Spill
Dial 2424
SPILL: Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. Eliminate sources of ignition if the chemical is
flammable. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g.,
use caution tape, barriers, etc.).
SMALL (<1 L) :If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material
for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
LARGE (>1 L): Evacuate the lab, Push Lab Emergency button and Dial SUNUM Facilities Management 2424 and for assistance.
CHEMICAL SPILL ON BODY OR CLOTHES: Remove clothing and rinse body thoroughly in emergency shower for at east 15 minutes. Seek
medical attention, Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management 2424 immediately.
CHEMICAL SPLASH INTO EYES: Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye
open. Seek medical attention, Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management at 2424 immediately.
Bio-hazardous Spill
Dial 2424
CONCENTRATED, 100 ml OF BSL- 2 OR SPILL IN PUBLIC AREA – Do not attempt to clean it up. Keep people from entering. Dial SUNUM
Facilities Management 2424 for assistance.
BIOHAZARDOUS SPILL ON BODY OR CLOTHES: Immediately remove contaminated clothing and place in a red biohazard bag. Wash with
antiseptic soap and water for at least 15 minutes. Seek medical attention. Dial Security and Health Center at 5555 immediately. Notify SUNUM
Facilities Management at 2424 immediately.
GENERAL BIOHAZARD SPILL CLEAN-UP: Use clean personal protective equipment appropriate for the lab Biosafety Level. Place absorbent
pads over area. Use appropriate disinfectant. Allow a minimum 20 minutes of contact time. Remove any sharps and discard in a sharps
container. Dispose clean-up materials as biohazardous waste for proper waste disposal. Remove protective clothing and segregate for
disposal.Wash hands with soap and water. Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management at 2424
immediately
INERT GAS LEAKAGE: Move the cylinder to an isolated, well-ventilated area. Notify SUNUM Facilities Management 2424 immediately. If large
or uncontrollable leak occurs, evacuate the area and Notify SUNUM Facilities Management 2424 immediately.
UNKNOWN, TOXIC,FLAMMABLE GAS LEAKAGE: Evacuate the lab, Push Lab Emergency button and Dial SUNUM Facilities Management
2424 and for assistance
INHALED: Remove to fresh air and Call 6666 for immediate medical attention.
CONTAMINATION: Remove clothing and rinse body thoroughly in emergency shower for at east 15 minutes. Seek medical attention, Dial
Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management 2424 immediately.
EYE CONTACT: Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. Seek medical
attention, Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management at 2424.
Gas-Leakage
Dial 2424
Earthquake
Stay calm. Remember to DUCK, COVER, AND HOLD.
If you are INDOORS, stay there. Do not run outside. Take cover under a heavy desk or table or in a doorway and protect your neck and
head.
If you are outdoors Move to a clear area if you can safely walk. Avoid buildings and trees.
After an earthquake, use caution, carefully evacuate your residence and go to the Emergency Assembly Point – FASS PARKING LOT.
Make sure you are accounted for before heading elsewhere.
19
Injury Statistics
Laboratory support injuries, 1993 - 1997*
The most common serious injuries: strains and fractures due
to falls and improper lifting of heavy objects.
Source: Howard Hughes Medical Institute, Office of Laboratory Safety.
All reported injuries are shown, without regard to severity.
Source: U.S. Department of Labor, Bureau of Labor Statistics(BLS)'s
2005
20
Accident/Incident/Hazard Form
Should be filled by EVERYONE
Complete form within 24 hours
of injury
Submit completed report to
your supervisor/FENS/SUNUM
Form is submitted to S.U
Health&Safety Department
21
Hazards and Safety
22
Chemical Hygiene Plan
Chapter 4.Chemical Hygiene Plan
4.1.1. Hazardous Chemical Definition
4.1.2. Hazard Identification
4.2. Training&Information
4.3. Standart Operation Prodedures
4.4. Protective Equipment
4.5. Laboratory Safety Equipments
4.6. Chemical Procurement, Distribution
Storage
23
Chemical Hygiene Plan, Ch. 4.1.1
Hazardous Chemical Definition
• Carcinogens
• Reproductive toxins
• Sensitizers
• Hepatotoxins
• Agents that act on the hematopoietic system
• Irritants
• Corrosives
• Neurotoxins
• Nephrotoxins
• Asphixiants
• Agents that damage the lungs, skin, eyes, or mucus membranes
24
Chemical Hygiene Plan, Ch. 4.1.2 Hazard Identification,
Risk Analysis
25
Chemical Hygiene Plan, Ch. 4.2
The content of the training programs will include the following:
•
•
•
•
•
•
University chemical hygiene policies; and
Applicable details of the University's Chemical Hygiene Plan.
Appropriate response in the event of a chemical emergency (spill, overexposure, etc.);
Physical and health hazards of various classes of laboratory chemicals;
Methods/procedures for handling and safely using chemicals present in laboratories;
Additional laboratory specific safety training is provided by the supervisor.
26
Chemical Hygine Plan, Ch.4.2.
Name of Chemical
Chemical Formula
or IUPAC NAME
CAS
registry
number
20x TE Buffer
2-Propanol
3,3′,5,5′-Tetramethylbenzidine
3,3′-Dithiodipropionic acid
Acetone
Acetone
Acetone
n/a
isopropyl alcohol
C16H19N
3,3′-Dithiodipropionic acid
Acetone
2-propanone
(CH3)2CO
not required
67-63-0
Acridine
C13H9N
Acrylamide
Agencourt AMPure XP
Ammonia Solution %25
Ammonia solution 32%
Ammonium acetate
Ammonium dihydrogen phosphate
Ammonium persulfate
Ammonium Persulfate
Ammonium sulfate
Aniline
APS
Baytubes C 150 P
Baytubes C 70 P
BCA Protein Assay Reagent (bicinchoninic acid)
Boric acid
Buffer EB (MinElute kit component)
Buffer PB (MinElute kit component)
Buffer PE (MinElute kit component)
Buffer pH=7.0,pH=10.01, pH=4.01
Buffered oxide etch
Cadmium chloride
Calcium chloride
Calcium nitrate tetrahydrate
Carboxymethyl Cellulose, Sodium Salt
Chitosan-LMW
Chloroform
Chloroform
Chloroform
Manufa Catalog
cturer
#
Invitrogen
Sigma
sigma
Sigma
Uzsol-A
Merck
P7589_B
I9516-500ML
860336-1G
109010-50G
260-94-6
Sigma
A23609-5G
C3H5NO
n/a
NH3
NH3
C2H7NO2
(NH4)H2PO4
Diazanium sulfate
Ammonium Persulfate
(NH4)2SO4
Aniline
(NH4)2S2O8
Multi-walled carbon nanotube
Multi-walled carbon nanotube
C20H10N2O4
Trihydroxidoboron
n/a
Guanidinium chloride (25-50%), propan-2-ol (25-50%)
n/a
Buffer pH=7.0,pH=10.01, pH=4.01
BOE or BHF
CdCl2
Calcium chloride
Ca(NO3)24H2O
Carboxymethyl Cellulose, Sodium Salt
Chitosan-LMW
CHCl3
CHCl3
CHCl3
79-06-1
none
1336-21-6
631-61-8
7722-76-1
7783-20-2
7783-20-2
7783-20-2
62-53-3
7727-54-0
7440-44-0
7440-44-0
979-88-4
10043-35-3
not required
50-01-1;
not required
Sigma
Beckman Coulter
MERCK
Merck
Sigma
Carlo Erba
Sigma
Sigma Aldrich
Sigma
Sigma Aldrich
Merck
Bayer MaterialScience
Bayer MaterialScience
Sigma
Sigma
Qiagen
Qiagen
Qiagen
A3553-500G
A63881
1.054.322.500
1.05426.2500
A1542-500G
419787
A3678-25G
N/A
10108-64-2
10043-52-4
13477-34-4
9004-32-4
9012-76-4
67-66-3
67-66-3
67-66-3
Technic France
Sigma
Sigma
Merck
Sigma Aldrich
Sigma Aldrich
Merck
Sigma
Merck
Citric acid
C6H8O7
77-92-9
sigma
C2404-100G
D-(+)-Trehalose dihydrate
C12H22O11
6138-23-4
Sigma
T9531-25G
Dimethyl sulfoxide
C2H6OS
67-68-5
Sigma
D2650-100ML
DL-Dithiothreitol
DNA 7500 kit
DNA standard (100ug/ml in TE buffer)
Ethanol
Ethanol
Ethidium bromide solution
Ethylene glycol
Ethylenediaminetetraacetic acid disodium salt dihydrate
GelPilot 5x loading dye (MinElute Kit component)
Glutaraldehyde solution
C4H10O2S2
n/a
n/a
Ethanol
Ethanol
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
C2H6O2
2,2',2'',2'''-(Ethane-1,2-diyldinitrilo)tetraacetic acid
Trometamol (1.0-2.5%)
C5H8O2
27565-41-9
not required
not required
64-17-5
64-17-5
1239-45-8
107-21-1
60-00-4
77-86-1
111-30-8
Sigma
Agilent
Invitrogen
Fisher Scientific
Uzsol-E
Sigma
sigma
Sigma
Qiagen
Sigma
43817-5G
5067-1506
P7589_C
BP2818-4
Glycine
GS FLX Titanium Control Bead Kit
GS FLX Titanium General Library Preparation Kit
GS FLX Titanium LV/MV/SV emPCR kit (Lib-A)
GS FLX Titanium LV/MV/SV emPCR kit (Lib-L)
GS FLX Titanium Maintenance Wash Kit
GS FLX Titanium Paired End Adaptor Set
GS FLX Titanium PicoTiterPlate Kit 70x75
GS FLX Titanium Rapid Library MID Adaptors Kit
GS FLX Titanium Rapid Library Preparation Kit
GS FLX Titanium Sequencing Kit XLR70
HEPES buffered saline
C3H7NO5P
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
56-40-6
not required
not required
not required
not required
not required
not required
not required
not required
not required
not required
7365-45-9
Sigma
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Roche 454 Sequencing
Sigma
G8898-1KG
05 470 129 001
05 233 747 001
05 619 (114/149/165) 001
05 618 (428/436/444) 001
04 932 358 001
05 463 343 001
05 233 682 001
05 619 211 001
05 608 228 001
05 233 526 001
51558-50ML
54827-17-7
1119-62-6
67-64-1
67-64-1
67-64-1
Package
Chemical
Inventory
Plastic Bottle
Plastic Bottle
Plastic Bottle
Plastic Bottle
Glass Bottle
Glass bottle
Plastic Bottle
A4418-500G
1012010500
23225
B6768-1KG
28004/28006
28004/28006
28004/28006

All chemicals in the Center (liquids,
gases and other materials) is
controlled

The list of allowed chemicals is published
Glass bottle

Only chemicals in the list is allowed in the
Center
Card box containing multiple vials
Plastic Vial
Amber glass bottle

Contact SUNUM management to add
your chemicals to the list

SUNUM maintains a full set of MSDS for
materials in the Center
Plastic bottle
Plastic Bottle
Plastic Bottle
Plastic bottle
Plastic bottle
Plastic bottle
Plastic Bottle
655198-5G
C1016-100G
1021210500
Plastic Bottle
Dark Glass Bottle
C2432-500ML
1.02445.2500
E1510-10ML
324558-100ML
E5134-100G
28004/28006
G5882-50ML
Plastic vial
Card box
Card box
Card box
Card box
Card box
Card box
Card box
Card box
Card box
Card box
containing multiple vials
containing multiple vials
containing multiple vials
containing multiple vials
containing multiple bottles
containing multiple vials
containing multiple vials
containing multiple vials
containing multiple bottles
27
Chemical Hygine Plan, Ch.4.2.3 Labeling
All chemicals (including solutions
and chemicals transferred from
their original containers) should
be labeled with their common
names, concentrations and
hazards. Additional information
such as: date received, date
opened, date of expiration
should also be recorded.
Label may also include: Proper
handling, Storage, Emergency response
28
Chemical Hygine Plan, Ch.4.2.3 MSDS
.MSDS
Includes
the following:
Product Information
Fire and Explosion
Toxicology
Health Effects
PPE
Storage
Leaks and Spills
Waste Disposal
First Aid
US&A (v 2/07)
Chemical Hygine Plan, Ch.4.2.3 MSDS
• The purpose of a Material Safety Data Sheet (MSDS) is to provide readily
accessible information on chemical substances commonly used in the science
laboratory or in industry.
• The MSDS should be kept on file and referred to BEFORE handling ANY
chemical. The MSDS can also be used to instruct students on chemical
hazards, to evaluate spill and disposal procedures, and to warn of
incompatibility with other chemicals or mixtures. These also provide you with
the First Aid measures for the stated chemical.
Materials Safety Data Sheet (MSDS) Websites
• http://www.fishersci.com
• http://www.vwr.com
• http:/www.hazard.com
• http://www.sigmaaldrich.com/
• Plus any vendor you order from must be able to provide an MSDS for
your product on request.
Hazard Placards
What are the health hazards?
What are the fire hazards?
What are the reactivity hazards?
Are there any special hazards?
4 = Severe Hazard
3 = Serious Hazard
2 = Moderate Hazard
1 = Slight Hazard
0 = Minimal Hazard
Hazard Placards
Perchloric Acid
Xylene
32
Additional Hazard Ratings
Hydrochloric Acid
Reagent Alcohol
7452 Accelerator
Acetic acid, glacial
33
Protective Clothing and Lab. Safety Equipment
•
•
•
•
Laboratory coat,
Gloves,
Protective eyewear
Closed toe shoes
are must to be worn in SUNUM laboratories
WHENEVER handling hazardous
chemicals.
• Additional personal protective
equipment, such as face shield, utility
gloves, aprons, and respirators, may be
necessary depending on an assessment
of the hazard and operation.
34
Protective Clothing and Lab. Safety Equipment
• Gloves
–
–
–
–
–
–
–
–
–
Cotton
Leather
Latex
Viton
Butyl
Neoprene
PVC
Nitrile
Other
35
Protective Clothing and Lab. Safety Equipment
Gloves
• Be sure you
know which
type of glove is
appropriate for
the chemical
you are using
Example of wrong
glove having been
used
36
Protective Clothing and Lab. Safety Equipment
Gloves
Glove
Intended Use Advantages
Material
• Good for biological and
• Poor for organic solvents
Latex exam
water-based materials
• Hard to detect puncture holes
style
• User acceptability
• Latex allergy issues
• Good for solvents, oils,
• Slightly more expensive than
greases, some acids and
latex
Incidental Contact
Incidental Contact
• Check “Chapter 4.4.6.1
Glove Selection Guide’’
• (Xylenes: Nitrile)
• (Perchloric Acid: Nitrile,
Neoprene or PVC)
Disadvantages
bases
Nitrile exam
• Clear indication of tears
style
and breaks
• User acceptability
• Good for solvents, oils,
• Not effective for
Utility style
greases, some acids and
halogenated and aromatic
Nitrile
bases
hydrocarbons
Extended Contact
• Can be washed and
– Solvex
Neoprene –
reused
Extended contact
• Poor for halogenated and
alcohols, fuels,
aromatic hydrocarbons
peroxides,hydrocarbons,
utility
and phenols
style
Butyl rubber
• Good for acids, bases
Extended contact
• Good for ketones and
esters
utility gloves
37
Protective Clothing and Lab Safety Equipment
Gloves
 NEVER leave the lab with
gloves
 Do not touch common
external surfaces such as
door knobs with gloves
 Remove soiled gloves: If
gloves become soiled or
contaminated during
experiment, stop, and
remove carefully
 Wash your hands and put
on new pair of gloves.
 If you double-glove,
remove soiled glove,
replace with new one, and
continue your work.
38
Protective Clothing and Lab Safety Equipment
W ear Eye P rotection W hen Using:
Glassware under reduced pressure
Cryogenic materials
Glassware under elevated pressure
Explosives
Caustics, Irritants or Corrosives
Biohazards
Radioactive Materials
UV Light
Toxic Chemicals
Carcinogens
Flammable Materials
Lasers
39
Protective Clothing and Lab Safety Equipment
Eye Protection
Wear goggles when using acids or
whenever there is potential for a splash
hazard
Wear safety glasses at other times
when laser light or chemicals are
present and when handling glassware
40
Storage
• Separate chemicals into
compatible groups
•
Designate separate
storage for highly toxic
chemicals
• Do not store more
chemicals than you will
need over a reasonable
time
41
Storage (continued)
Store corrosive, toxic, and
highly reactive chemicals in
a well-ventilated area
Store chemicals that can ignite at room
temperature in a flammables cabinet
42
Storage (continued)
Never store liquid
hazardous chemicals
above eye level
Chemicals stored overhead
should be kept on shelves
with raised edges.
43
Storage (continued)
• Don’t store water reactive
chemicals under sink
• Store heavy chemical
containers on lower
shelves and not on floor
44
Storage (continued)
Never store highly reactive chemicals longer
than 6 months. Examples:
• Picric acid
• Ethyl ether (diethyl
ether)
• Phosphorus
• Sodium metal
• Potassium metal
45
Examples of Poor Storage
•Never store a
chemical with a
missing or obscured
label
46
Leaks and Spills
•
•
•
•
•
•
•
•
•
Alert people in immediate area of spill.
Increase ventilation in area of spill (open
windows, turn on hoods).
Wear protective equipment, including safety
goggles, gloves, long-sleeve lab coat and closed
toe shoes.
Avoid breathing vapors from spill.
Use appropriate kit to neutralize and absorb
inorganic acids and bases. Collect residue, place
in container, and dispose as hazardous chemical
waste.
Call Lab Supervisors for disposal information, if
necessary.
For other chemicals, use appropriate kit or absorb
spill with vermiculite, dry sand, diatomaceous
earth, spill pads, or paper towels.
Collect residue, place in container, and dispose as
chemical waste.
Clean spill area with water.
47
Chemical Spills -- Minor
• Spill considered
minor only if
person who
spilled it is
familiar with
chemical, knows
associated
hazards and
knows how to
clean up spill
safely
Chemical Spills -- M inor
Minor Spills
Alert coworkers and
proceed to clean up
spill
Contact SUNUM
Facilities
Management &
University Services
for disposal of spill
clean-up materials
49
Chemical Spills -- Major
Major Spills
• Anything beyond minor spill
• Anything requiring help from
outside of the lab group
Chemical Spills -- Major
•
•
•
•
•
•
•
Attend to injured or contaminated persons
and remove them from exposure.
Push the Emergency Button next to lab
door.
Alert people in the laboratory to evacuate.
If spilled material is flammable, turn off
ignition and heat sources. Place spill
cleanup material over spill to keep
substance from volatilizing. An MSDS
contains special spill clean-up information
and should also be consulted.
Call SUNUM Facility Management 2424 or
5555 any time.
Close doors to affected area.
Have a person with knowledge of the
incident and laboratory available to answer
questions from responding emergency
personnel.
Minor Spill Response
• Be prepared for emergencies
• Ask your Lab. Supervisors for
Emergency Spill Kits.
Biohazardous Spill Cleanup Procedures
• Sample of spill
cleanup plan
Hazardous Waste Disposal Rules
• Minimize
• Containerize
• Label
See Hazardous Waste Disposal
Rules:
Health and Safety Handbook,
Chapter 12.
Hazardous Waste Disposal Rules
• Minimize
• Containerize (Chemical
waste in general, and hazardous
waste in particular, must be
stored in sturdy container, free of
leaks.)
• Label
Hazardous Waste Disposal Rules
• Minimize
• Containerize
• Label (All hazardous
waste containers must
be properly labeled.)
Hazardous W aste Disposal Rules
Do not use chemical
symbols or
abbreviations when
labeling your
containers
57
Hazardous Waste Disposal Rules
Autoclave Infectious
Waste
– or Contact SUNUM
Facilities Management
(2424) for disposal
Sharps Disposal
Put sharps in punctureresistant, leak-proof, sharps
container.
Label the container with the
word, "SHARPS”.
If not biohazardous, deface
any "BIOHAZARD"
markings or symbols.
Seal container tightly.
•Contact SUNUM Facilities Management for pickup.
59
First Aid -- Small Cuts, Scratches
Small cuts and scratches:
• Cleanse area with soap and water
• Place a clean dressing over the
wound
• University Health Center is
available to evaluate patients for
minor injuries which may require
sutures, tetanus booster, etc.
60
First Aid - Wounds
Wounds -- Significant bleeding
 Call 6666 IMMEDIATELY




Calm and reassure victim
Lay victim down. (Reduces chance of fainting)
DO NOT remove any objects impaled in victim
Put direct pressure on wound with a sterile bandage or clean
cloth
 If direct pressure does not control bleeding, elevate wound above
the heart if possible
 If bleeding is severe elevate victims legs about 12 inches, and
cover victim with a blanket
 DO NOT APPLY TOURNIQUET
61
First Aid - Chemical Burns (Eyes)
• Forcibly open eyelids to ensure
effective washing behind eyelid
• Wash from nose out to ear to avoid
washing chemicals back into eye or
into an unaffected eye
 Flood eyes and eyelids with
water/eye solution for
minimum of 15 minutes
First Aid - Chemical Burns (Eyes)
Remove contact
lenses immediately
to rinse eyes of
harmful chemicals
Cover eyes with
clean or sterile
gauze
Call 6666 or
proceed directly to
the Emergency
Room
Make sure you know the
location of the nearest
eyewash station
63
Chemical Burns - Skin
Remove any
contaminated
clothing
Chemicals may
collect in shoes,
remove shoes, if
appropriate
Rinse area with
large quantities of
water for at least 15
minutes (sink,
shower or hose)
64
First Aid - Chemical Burns - Skin
 DO NOT apply burn
ointments/spray to affected
areas
 Cover with dry clean or sterile
material
 For large affected areas, call
6666
 If possible, bring the container
of the chemical and/or its
MSDS along with you to the
evaluating medical facility.
REPORT ALL INJURIES,
no matter how small
(investigations of near
misses lead to fewer
future injuries)
65
First Degree Thermal Burns
In general first degree burns have
redness only, no blister or more
extensive tissue damage.
Run cool water over area of burn
or soak in cool water for at least 5
minutes.
Use only WATERJEL-Burn Kits
DO NOT APPLY ANY OINTMENTS,
SPRAYS OR SALVES.
Note: Burns can at first appear to be first degree, but blisters may develop
over the next several hours. When in doubt, seek medical attention
66