Health and Safety Handbook
Centre for Public Health
September 2015
Please read this document carefully and familiarise yourself with its contents.
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Centre for Public Health
Health and Safety Manual including:

CPH Laboratories, Institute of Pathology Building, RVH

Nephrology Research Laboratories, ‘A’ Floor, BCH

Clinical Research Facility, U Floor, BCH

Institute of Clinical Science, Block B, RVH
Document History (Replaces all documents listed)
Title and Author
Author
Year
Nephrology Health and Safety Rules
AJ McKnight
2006
Nephrology Health and Safety Rules
AJ McKnight, J Kilner, P Erwin
2007
Nephrology Health and Safety Rules
AJ McKnight, J Kilner
2007
Nephrology Health and Safety Rules
AJ McKnight, J Kilner
2009
CPH Laboratory Manual
C McMaster
2010 Version 1
Nephrology Laboratory Manual
C McMaster, J Kilner
2010 Version 1
CPH Laboratory Manual
C McMaster
2011 Version 2
CPH Laboratory Manual
C McMaster
2012 Version 3
CPH Laboratory Manual
C McMaster, J Kilner
2012 Version 4
CPH Health and Safety Handbook
C McMaster, J Kilner
2013
CPH Health and Safety Handbook
C McMaster, J Kilner
2014 Version 1
CPH Health and Safety Handbook
C McMaster, J Kilner
2014 Version 2
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Abbreviations used in the CPH Handbook
ADR
AOR
BCH
BHSCT
BLO
CCTV
COSHH
CPH
CPR
CTU
DHL
DSE
ERA
GHS
GMO
H&S
HMRC
HSE
HTA
IATA
ICSB
IOP
LP
MSDS
MTA
NI
PAT
PDA
PPE
PRA
QUB
RIDDOR
RPS
RSI
RVH
SMDBS
SOP
STDU
TRA
UNECE
UV
VDU
WEEE
WEL
European Agreement on International Carriage of Dangerous Goods by Road
Artificial Optical Radiation
Belfast City Hospital
Belfast Health and Social Care Trust
Buildings Liaison Officer
Closed Circuit Television
Control of Substances Hazardous to Health
Centre for Public Health
Cardiopulmonary Resuscitation
Core Technology Unit
Adrian Dalsey, Larry Hillblom and Robert Lynn – DHL founders
Display Screen Equipment
Equipment Risk Assessment
Globally Harmonised System
Genetically Modified Organisms
Health and Safety
Her Majesty’s Revenue and Customs
Health and Safety Executive
Human Tissue Act
International Air Transport Association
Institute of Clinical Science, Block B
Institute of Pathology
Laboratory Procedure
Material Safety Data Sheet
Material Transfer Agreement
Northern Ireland
Portable Appliance Test
Portable Digital Assistant
Personal Protective Equipment
Procedure Risk Assessment COSHH Form
Queen’s University Belfast
Reporting of Injuries, Diseases and Dangerous Occurances
Radiation Protection Supervisor
Repetitive Strain Injury
Royal Victoria Hospital
School of Medicine, Dentistry and Biomedical Sciences
Standard Operating Procedure
Staff Training and Development Unit
Task Risk Assessment
United Nations Economic Commission for Europe
Ultra Violet
Visual Display Unit
Workplace Electrical and Electronic Equipment
Workplace Exposure Limits
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Index of Contents
Title Page
Document History
Abbreviations used in the Handbook
Index of Contents
Page 1
Page 2
Page 3
Page 4
Section 1
Introduction
1.1 General Introduction
1.2 H&S Handbook information
1.3 EMERGENCY Numbers
Page 9
Section 2
Health and Safety Organisation and Management
Page 12
2.1 University Health and Safety Policy
2.2 CPH Health and Safety Policy
2.3 University Health and Safety Structure
2.4 CPH Health and Safety Structure
2.5 CPH Health and Safety Committee Terms of Reference
2.6 CPH H&S Committee composition
2.7 CPH Health and Safety Arrangements
2.7.1 Health and Safety Information and Training
2.7.2 Health and Safety Manual Review
2.7.3 Health and Safety Training Records
2.7.4 Monitoring of Safety in the workplace
2.7.5 COSHH/Risk Assessments
2.7.6 Electrical Safety
2.7.7 Fire Safety Procedures
2.7.8 Display Screen Equipment
2.7.9 Lone Working
2.7.10 Manual Handling
2.7.11 Accident Reporting
2.7.12 First Aid
2.7.13 New and Expectant Mothers
2.7.14 Staff with Disability
2.7.15 Visitors and Maintenance Engineers
2.7.16 CPH Health and Safety Responsible Persons
2.7.17 Human Tissue Act Compliance
2.7.18 Infectious Agents and GMO’s
2.7.19 Controlled Substances/Flammable Liquids/Gases
2.7.20 Strategic Risk Register
2.7.21 Major Incident Reporting
2.8 CPH Health and Safety Development Plan
Section 3
Personnel
Page 24
3.1 Nominated personnel, roles and responsibilities
3.2 Induction, training and education
3.2.1 Safe use of Display Screen Equipment (DSE)
3.2.1.1 Getting comfortable
3.2.1.2 Keyboard Use
3.2.1.3 Using a mouse
3.2.1.4 Reading the screen
3.2.1.5 Posture and Breaks/ work planning
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3.2.1.6 Further Information
3.2.1.7 Portable Display screen equipment
3.2.1.8 Provision of eyesight test
3.2.2 Data Handling
3.2.2.1 Data Protection Act
3.2.2.2 Lab samples
3.2.2.3 Lab / record books
3.2.2.4 On-site digital analysis and storage
3.2.2.5 Off-site digital storage
3.2.2.6 E-mail
3.2.2.7 Back-up copies
3.2.2.8 Reporting security breach
3.3 Staff Health and Wellbeing
3.3.1 Staff Wellbeing
3.3.2 New and expectant mothers
3.3.3 Tidiness and Hygiene
3.3.4 First aid
3.3.5 Smoking
Section 4
Premises and Environment
Page 30
4.1 Areas covered by this manual
4.2 Assessment that CPH laboratories are fit for purpose
4.3 Access and security
4.3.1 Buildings access and security
4.3.2 Using the lift
4.3.3 Personal Security
4.3.4 Bicycle Security
4.3.5 Security in the car
4.4 Main Hazards identified in CPH
4.5 Fire fighting equipment
4.6 Lone working
4.6.1 CPH Lone Working Policy
4.6.2 Permit to lone work form
4.6.3 Additional lone working arrangements
4.6.4 Lone working Risk Assessment
4.7 Notices and warning signs used in CPH
4.8 Local rules for visitors, cleaning staff and service engineers
4.9 Monitoring, control and recording of environmental conditions
4.9.1 Reporting of Faults
4.9.2 Noise in the workplace
4.9.3 Removal of waste
4.9.3.1 Furniture and WEEE
4.9.3.2 Recycling
4.9.3.3 Confidential
4.9.3.4 Domestic
4.9.3.5 Laboratory
4.10 Storage facilities for hazardous substances
4.11 Permit to work
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Section 5
Equipment and Materials
5.1 Fume Cupboards
Page 45
Section 6
General Health and Safety – lab only
6.1 Introduction
6.2 Staff health
6.3 Good housekeeping
6.4 Hygiene for laboratory users
6.4.1 Personal belongings
6.4.2 Keep objects away from the mouth
6.4.3 Hand washing
6.4.4 Food and drink
6.4.5 Smoking
6.4.6 Cosmetics
6.4.7 Mobile phone use
6.5 Personal Protective Clothing and Equipment
6.5.1 Laboratory coat
6.5.2 Protective gloves and glove choice
6.5.3 Eye and face protection
6.5.4 Respiratory protection
6.5.5 Footwear
6.5.6 Ear protection
6.6 Safety showers
6.7 Hair/ jewellery
6.8 Using DSE
6.9 Working with chemicals
6.9.1 Chemical hazards in common use
6.9.2 Labelling containers
6.9.3 Labelling Research
6.9.4 Handling of chemicals
6.9.5 Flammable solvents
6.9.6 Highly reactive substances
6.9.7 Dilution of Concentrated acid
6.9.8 Carcinogens
6.10 Working in Tissue culture
6.11 Electrical Safety
6.12 Moving and handling
6.12.1 Lifting and carrying, manual handling
6.12.2 Glassware and aerosols
6.12.3 Sharps
6.13 Disinfection
Page 47
Section 7
First Aid
7.1 Basic advice about First Aid at work
7.2 Chemical contamination
7.3 Burns and scalds
7.4 Electrocution
7.5 Injury from compressed gases – cold burn
7.6 Possible exposure to infection
7.7 Call an ambulance
Page 62
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7.8 Accident reporting
Section 8
Fire Prevention and Control
8.1 The fire alarm
8.2 Action in the event of a fire
8.3 Training in fire safety
8.4 Fire fighting equipment
8.5 Fire Officers
8.6 Fire Controller and fire Wardens
Page 67
Section 9
Spillages Policy
9.1 Scope Purpose and Responsibility
9.2 Action in the event of an oil / chemical spill
9.2.1 Small spillage
9.2.2 Large spillage
9.2.2.1 Liquid
9.2.2.2 Powder
9.2.3 First Aid
9.2.4 Keeping a record
9.2.5 Summary
9.3 Action in the event of a biological spill
9.4 Action in the event of a mercury spill
9.5 Action in the event of a radioactive spill
9.6 Action in the event of a cryogenic spill
Page 71
Section 10
Control of Substances Hazardous to Health (COSHH)
10.1 What is COSHH?
10.2 Why is COSHH important?
10.3 Completing a COSHH risk assessment (PRA)
10.4 COSHH chemicals (A-Z)
Page 77
Section 11
Risk Assessment
11.1 General
11.2 Completing a new Risk Assessment
11.3 Equipment Risk Assessment
11.4 Task Risk Assessment
Page 79
Section 12
Laboratory Procedures
12.1 Laboratory Procedure Document Control
12.2 Equipment Standard Operating Procedures
Page 82
Section 13
Radiation Safety Procedures
13.1 Ionising radiation, supervised areas and RPS
13.2 Ionising radiation Safety Policy
13.3 Artificial Optical Radiation
13.4 UV Transilluminators
Page 83
Section 14
Compressed gases
14.1 Gas Safety and liquid nitrogen
14.2 Handling liquid nitrogen
14.3 PFTE tape and oxygen service
Page 84
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14.4 Gas equipment safety checks
14.5 Gas welding and cutting equipment safety checks
Section 15
Laboratory Disposal Policy
Page 86
15.1 Classification of waste
15.1.1 Microbiological (RVH site)
15.1.2 Cell and tissue culture procedures (RVH site)
15.1.3 Human blood, tissue and other secretions
15.1.3.1 RVH site
15.1.3.2 BCH site
15.1.4 DNA and DNA containing products (BCH site)
15.1.5 Sharps
15.1.6 Chemicals
15.1.7 Solvents and alcohols
15.1.8 Broken glassware
15.1.9 Contaminated lab coat
15.1.10 Spillages
15.1.11 Furniture, electrical & electronic equipment WEEE
15.1.12 Recycling
15.1.13 Confidential
15.2 Waste disposal containers
15.2.1 Yellow clinical waste bag
15.2.2 Sharps bin with blue lid
15.2.3 Sharps bin with purple lid
15.2.4 Yellow rigid container with yellow lid
15.2.5 Yellow rigid container with black lid
15.2.6 Clear autoclave bag
15.2.7 Domestic waste bag
15.2.8 Glass waste box
Section 16
Procedure for Specimens
Page 102
16.1 New studies
16.2 Human Tissue Act
16.3 Sample collection
16.4 Sample reception
16.5 Sample storage
16.5.1 Ultralow Temperature Storage
16.5.2 Refrigeration at 4°C
16.6 Transfer of samples to another location
16.6.1 Material Transfer Agreement (MTA)
16.6.2 Sample transport
16.6.3 Procedure for shipping in dry ice (RVH site)
16.6.4 Dangerous goods
16.7 Sample disposal
Section 17
Appendices
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Section 1: Introduction
1.1 General Introduction
The information in this document is offered as a guide to help you think and act
safely in the workplace.
Remember most accidents can be avoided by thinking first and acting second.
It is university policy and should be a duty of care for all staff to act positively to prevent:
injury, ill-health, environmental damage, equipment damage or loss arising from work
carried out within offices and laboratories, in accordance with the Health and Safety at
Work (N.I.) Order of 1978 and subsequent COSHH regulations.
This moral duty of care is fully supported by law.
The health and safety guidelines that follow apply to the entire Centre but particularly to
our laboratories.
Further information can be obtained from your Line Manager, Floor Managers or the
University Safety Service. Tel 9097 3674
Our priorities must always be:
1. Accident prevention.
2. Damage limitation. Know what to do when an incident occurs.
3. Learning from accidents or ‘near misses’.
All employees and students are required by the University:
 to take reasonable care of their own health and safety and that of others who
may be affected by their own acts or omissions;
 to familiarise themselves with any relevant Health and Safety Policies and/or
Procedures;
 to co-operate with the University and its officers to enable the University to
comply with its statutory obligations;
 to use equipment, machinery, plant and substances in accordance with the
instructions and training that they have received;
 to inform their manager or supervising member of staff of any dangers or
shortcomings in the health and safety arrangements, even if there is no risk
of immediate danger;
 to not intentionally or recklessly misuse or interfere with anything provided in
the interest of health, safety and welfare.
Failure to comply or to co-operate with any health and safety measure
required by the University may lead to disciplinary action being taken.
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As your Employer: the University has a duty to protect you and to provide you with
relevant health and safety information.
As an Employee: you have a duty to co-operate with the University, to take reasonable
care to protect yourself and others who may be affected by what you do or do not do and
not to interfere with or misuse anything provided for your health, safety and welfare at
work.
Further detailed information on University Policies and Procedures can be found on QUB
occupational Health and Safety Website:
http://www.qub.ac.uk/directorates/HumanResources/OccupationalHealthandSafety/Healt
handSafetyPolicy/
Remember: Your health, safety and welfare at work is protected by law.
1.2 Health and Safety Handbook Information
This document is mandatory reading for all categories of staff and research
students in the Centre for Public Health. It is offered as a guide to help you to
think and act safely and thus avoid accidents to yourself and to others working
around you.
You will be required to sign a register stating that you have read both the CPH
Induction Booklet and this Handbook.
1.3 Emergency Telephone Numbers
Medical, Security, Crime:
Emergency Contact
Emergency Crash Line or other Medical Emergencies
BCH Tower Security
QUB Security
RVH Security
Fire/Police/Ambulance
Police, Non-emergency
Poisons Information Centre
Telephone
Number
6666
028 950 42000
028 90 975099
33553
9-999
9-101
028 9504 0558
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Buildings and Estates Emergency:
Site
Out of Hours Emergencies
Telephone Number
RVH
Plumbers and Electricians.
9097 5099
Contact: QUB Security, Porter’s Lodge,
Lanyon Building
BCH
Duty engineer.
Dial 0
Contact: Switchboard operator and ask
for the duty engineer
University Safety Services:
Contact
Telephone number
e-mail address
University Safety Office
90 97 4681
Occupational Health Service
University Biological Safety
Officer
90 97 5520
occhealth@qub.ac.uk
90 97 4610 Mon - Wed d.norwood@qub.ac.uk
90 97 2473 Thu - Fri
safety@qub.ac.uk
Trained First Aiders:
Site
RVH
First Aiders
Location
Telephone
Number
Lesley Hamill
1st Floor ICSB
028 906 32219
Georgie Holmes
1st Floor ICSB, Admin Office
028 906 32608
Eimear Barrett
BCH
nd
Floor ICSB
028 906 32219
nd
2
Sheree Hanna
2
Floor ICSB
028 906 33078
Colin Fox
Mulhouse Building
028 906 32724
Samantha Jameson
Mulhouse Building
028 906 32573
Christine Belton
Inst of Pathology
028 909 76295
Sarah Gilchrist
Inst of Pathology
028 909 76295
AJ McKnight
A Floor BCH
028 906 38460
Jill Kilner
A Floor BCH
028 906 38458
Pauline Erwin
A Floor BCH
028 906 38459
Julie Wilson
CRF U Floor BCH
028 950 49363
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Section 2: Health and Safety Organisation and Management
2.1 University General Statement of Health and Safety Policy
Queen’s University of Belfast is committed to the highest standards of excellence in education
and research and this commitment applies equally to the management of the health and safety
for staff, students and others who may be affected by matters within its control. The University
will adopt best practice in all areas of health and safety management through continuous
improvement in the control of risk.
The Senate, as the governing body of the University, has ultimate responsibility for the Health
and Safety Policy and for monitoring the effectiveness of supporting systems.
The Vice-Chancellor has executive responsibility for ensuring the implementation and monitoring
of the Health and Safety Policy, including the provision of adequate resources and for ensuring
that the University complies with all relevant statutory requirements and associated codes of
practice.
Health and safety is an integral part of the management of the University’s undertakings and a
core function of University Managers who, through the organisational structures, will take all
reasonably practicable steps to ensure that:· the risks to health and safety throughout its undertaking are properly assessed;
· there are safe systems of work in place;
· there is a safe environment in which to work;
· managers and supervisors have appropriate knowledge and competence to deal effectively
with health and safety issues;
· staff, students and others are given adequate supervision, information, instruction and training
in order to carry out their work and studies safely
· there is an effective mechanism through which Safety Representatives are consulted in good
time on health and safety matters.
The Occupational Health and Safety Service will provide all necessary professional support and
advice to University Managers.
Staff and students have a duty to take reasonable care of their own health and safety and that of
others who may be affected by their acts or omissions. Staff and student co-operation and
commitment is essential to ensure successful implementation of the health and safety policy.
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2.2 Centre for Public Health Statement of Safety Policy
It is the Centre’s policy to ensure, so far as reasonably practicable, the health, safety and
welfare at work of all its staff and students in accordance with the University Safety Policy, the
relevant statutory requirements and the code of practice ‘Safety in Universities’ published by the
Committee of Vice-Chancellors and Principals of the Universities of the United Kingdom.
In accordance with the general policy stated above, it is the Centre’s policy, so far as is
reasonably practicable:
 to ensure that plant and systems of work are safe and without risk to health
 to ensure safety and absence of risks to health in connection with the use, handling,
storage and transport of articles and substances
 to ensure that such information, instruction, training and supervision is provided for the
health and safety at work of staff and students
 to ensure that all places of work within the centre are maintained in a safe condition and
without risk to health and to provide and maintain means of access and egress that are
safe and without risk
 to provide a working environment for staff and students that is safe, without risk to health
 to provide personal protective clothing and equipment as is necessary to protect the
health and safety at work of staff and students





to promote the development of a positive attitude and interest in safety amongst staff and
students, including responsibility for personal safety and the safety of others
to maintain a detailed list of the membership of the Centre Safety Committee and its
functions
to prepare and update as necessary, the committee terms of reference which shall be
readily available
to update the local rules as and when required and to disseminate such changes to all
staff
to maintain a register of all CPH Health and Safety information, records and audits for
inspection and consultation by the Chairman of the University Safety Committee and
Head of the School of Medicine, Dentistry and Biomedical Sciences.
The centre has established a Safety Committee to advise members of the school on all matters
relating to safety and to oversee the implementation of this policy.
Membership of the centre committee includes representatives of academic and technical staff
including student representation.
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2.3 University Health and Safety Structure
Senate
Retains primary responsibility for Health and Safety for all
staff, students and visitors
The Vice-Chancellor
Is directly accountable to Senate and has ultimate executive
responsibility for the health and safety at work of staff, students
and visitors
Director for Safety
The registrar/Chief Operating Officer has been designated by
the Vice Chancellor as the Director with executive
responsibility
Deans
Review management arrangements for Health and Safety
within schools
Heads of School
Health and Safety Management is the responsibility of the
Head of School
School Safety Committees
Various health and safety committees are in place for
management review, policy development, consultation with
employees and the provision of specialaist technical advice
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2.4 Centre for Public Health Safety Structure
Centre Directors
Have primary responsibility for Health and Safety for all staff, students and
visitors within their Centre
Centre Safety Committees
It is the responsibility of the Centre Director to ensure that an effective
health and safety forum exists for consultation with staff and students. The
Centre for Public Health has an active committee that meets regularly and is
managed by a chairperson appointed by the Centre Director.
Chair of the H & S Committee
Dr J McEneny arranges and chairs six monthly committee meetings and
represents CPH on the School of Medicine, Dentistry and Biomedical
Science Health and Safety Committee.
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2.5 CPH Health and Safety Committee Terms of Reference
General statement of purpose:
To provide a consultative forum that will effectively address the Health and Safety requirements of
Queens University Belfast.
To ensure that all aspects of laboratory and office safety are planned, organised, controlled,
maintained, audited and reviewed.
Aims:
To continually endeavour to update and improve communication of Health and Safety requirements
throughout the Centre for Public Health (CPH).
To that end, before the end of March each year the Chairperson, along with the Co-ordinators, (Centre
Administration Manager and the Centre Chief Technician), will review the past year and make plans
for the incoming year, scheduling annual workplace safety inspections: one announced and one unannounced.
Duties:
a) Formulation and revision of CPH Health and Safety policy, in accordance with Queens
University requirements.
b) To review Health and Safety processes and procedures to ensure Centre compliance with
current University policy.
c) To oversee the collection of relevant Health and Safety information as requested by the School
of Medicine, Dentistry and Biomedical Science, (SMDBS), and University Safety Service,
(USS).
d) To monitor Health and Safety compliance and provide information required for the University
Annual Audit.
e) To undertake any other Health and Safety review or activity as requested by SMDBS or USS.
f) To report all Health and Safety incidents immediately to SMDBS via the Centre Admin
Manager, in addition to completion of the required AC1 Accident/Dangerous Occurrence/Near
Miss Report Form, forwarded to USS.
g) To receive reports of non-compliance and take appropriate action to resolve these
immediately.
Reports to:
The chairperson or elected representative (normally the Centre Administration Manager) reports
directly to the Centre Director, SMDBS Health and Safety Committee and the USS Manager.
Receives reports from:
The Centre Director, SMDBS Safety Manager and USS Manager.
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2.6 Centre for Public Health Safety Committee Composition
Committee meetings:
Meetings are held every six months, with additional convened meetings if required.
Minutes:
Minutes of the meetings will be recorded by the secretary of the CPH Director.
Transcription of these minutes will be completed and forwarded to all committee members within one
week of the last meeting.
Agendas:
The chairperson will notify meeting agendas by email one month in advance and will receive additional
items up to five working days before the date of the proposed meeting.
Roles assigned and person(s) appointed responsible:
Role
Person(s) Responsible
Chairperson Appointed by Centre Director
Health and Safety Co-ordinators
Dr Jane McEneny
CPH Centre Admin. Manager and Chief Technician
Niamh McElherron and Cyril McMaster
ICSB representative
Niamh McElherron
IOP representative
Christine Belton
Laboratory safety information update officer
Christine Belton
IOP shared building co-ordinator
Cyril McMaster
BCH representatives
Dr AJ McKnight and Jill Kilner
BCH shared building co-ordinator
Jill Kilner
Mulhouse representative
Colin Fox
Postgraduate student representatives
Kayleigh Griffiths and Desiree Schliemann
Union representative Invited
John Liddle
COSHH supervisor appointed by Chief Technician
Biological safety co-ordinators
Appointed by Centre Director
Christine Belton
Dr AJ McKnight (BCH site)
Cyril McMaster (RVH site)
Radiation Protection Supervisor / Deputy
Dr S Gilchrist / C Belton
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2.7 CPH Health and Safety Arrangements
While Health and Safety is the responsibility of all staff, the Centre Director has delegated the duty to
the Centre Health & Safety co-ordinators: i.e. the Chairperson of the H&S Committee, the Centre
Administrative Manager and the Chief Technician, and their nominees to take responsibility for Health
and Safety compliance within the centre.
The following is a list of these responsibilities and actions for implementation.
2.7.1 Health and Safety Information and Training
It is the duty of Line Managers, Floor Managers and Principal Investigators to
ensure that all new staff and students receive H&S information using the Centre Induction Booklet
and the Centre Handbook.
Everyone should also be made aware of the University’s NO SMOKING POLICY, and of their
responsibility in switching off lights and electrical equipment, particularly at weekends and holidays.
Appendix F
The University Safety Service in association with the Staff Training and Development Unit (STDU)
offers a range of courses. Full details about the courses and course registration can be obtained
through Queen's Online via - iTrent.
2.7.2 Health and Safety Manual Review
The Chief Technician/H&S Co-ordinator will review the Health and Safety Manual and the Induction
Booklet annually in consultation with the other H&S co-ordinators.
2.7.3 Health and Safety Training Records
The Centre Administrative Manager records all H&S training courses attended.
It is the individual’s responsibility to forward this information.
The H&S laboratory safety information update officer, Christine Belton, is responsible for ensuring staff
and student training records are updated during the months of October, February and June.
2.7.4 Monitoring of Safety in the workplace
The Health and Safety co-ordinators conduct one annual announced inspection of offices and
laboratories and one un-announced inspection using a designated checklist. The results are recorded
on the Centre shared drive.
2.7.5 COSHH/Risk Assessments
The Centre has appointed Christine Belton as COSHH supervisor:
to have primary responsibility for the maintenance of the Centre COSHH chemical database.
to maintain and update the Centre MSDS product information database
to ensure along with other floor managers and supervisors that safe handling of substances is
followed using Standard Operating Procedures, Procedure Risk Assessments and Task Risk
Assessments.
to maintain and update all risk assessments and ensure that these are signed by the relevant staff and
students.
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An audit of COSHH compliance for each laboratory will be performed alongside the updating of
training records by the COSHH supervisor and a complete revision of all Risk Assessments will be
conducted every five years or earlier if necessary.
2.7.6 Electrical Safety
PAT testing is co-ordinated by the Centre Administrative Manager and the Buildings Liaison Officer. It
is their responsibility to ensure annual testing of all electrical equipment and that any faulty equipment
is removed from use until repaired or replaced. PAT test reports are recorded on the centre shared
drives and also forwarded to the School Office.
2.7.7 Fire Safety Procedures
The Premises Fire Officer is responsible for arranging and recording the following Fire Safety
Requirements on Share Point:
Weekly fire alarm testing
Weekly, monthly and annual fire warden checks according to University Regulations.
Fire Warden training to be updated at 5 year intervals
Annual Emergency Evacuation
BCH Site: Fire alarm testing and evacuation procedure according to BHSCT regulations. Fire warden
checks are arranged by Regional Genetics fire warden and BHSCT fire officer.
2.7.8 Display Screen Equipment
All new staff are required to undertake online DSE Assessment and report completion to the
Administrative Manager for recording purposes.
Centre DSE trained assessors are available to advise or help with staff assessments as requested.
2.7.9 Lone Working
Signed Lone Working logs sheets are filed in the Chief Technicians office and these are reviewed
quarterly by the Centre Director and CPH Management team.
2.7.10 Manual Handling
STDU Training course in manual handling and lifting is recommended to all staff.
Laboratory specific training courses for CPH are organised as required and records are maintained in
the Chief Technicians office.
Training in the handling of gas cylinders is provided by the Chief Technician.
2.7.11 Accident Reporting
In addition to completion of a Centre accident log book and an AC1 form, all accidents and near
misses are reported to the Centre Administrative Manager for recording and reporting to the School
office.
All accidents are reported to the Centre H&S Committee for review and action if considered
necessary.
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2.7.12 First Aid
The Centre Administrative manager requires an annual update on the number of staff who are first aid
trained. A record of training and refresher dates is maintained by the Chief Technician and stored on
the Centre shared drive.
2.7.13 New and Expectant Mothers
The responsibility to report pregnancy lies with the member of staff or student. Once the line manager
is informed, the University Safety Service is contacted and an individual Risk Assessment is
conducted. In addition, all other Risk Assessments are revised where appropriate for that member of
staff or student.
Every effort is made to accommodate new mothers requirements in accordance with University policy.
2.7.14 Staff with Disability
Upon formal notification of disability and in accordance with guidance from the University Occupational
Health Service and USS, the Centre will make all the necessary adjustments as advised and essential
for each member of staff with a disability. The BLO will liaise with the University Estates Department to
ensure any adjustments to the workplace are facilitated in accordance with individual requirements.
2.7.15 Visitors and Maintenance Engineers
The Centre is required to take reasonable care to ensure the health and safety of visitors and
maintenance/service engineers. Visitors have to request permission to enter our workplaces and will
be accompanied by a member of staff at all times.
Visitors to our laboratories are required to sign a visitor log book (RVH site only) and Service
Engineers are required to sign a de-contamination/safe to work permit before commencing work.
Visitor log sheets are kept on file by the chief technician.
Access to our Radiation laboratory is restricted and controlled by the Radiation Protection Supervisor
who maintains signed records of all visits to this hazardous area.
Risk assessments for Visitors and Maintenance staff are posted in the relevant areas.
Visitor passes can be obtained from the Chief Technician in Pathology (RVH site).
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2.7.16 CPH Health and Safety Responsible Persons
Role
Person(s) Responsible
Workplace management co-ordinator
Niamh McElherron
Fire Premises Officer and Deputy
Cyril McMaster/ Niamh McElherron
Fire Evacuation Controller/Deputy ICSB
Niamh McElherron/Cyril McMaster
Fire Evacuation Controller/Deputy IOP
Cyril McMaster/Niamh McElherron
Fire Evacuation Controller Mulhouse
Safety Officers/COSHH compliance
Colin Fox
Prof Liam Murray, Niamh McElherron and
Cyril McMaster
Christine Belton for RVH site and Jill Kilner
for BCH site.
COSHH Supervisor
Christine Belton
Radiation Protection Supervisor/Deputy
Dr Sarah Gilchrist/Christine Belton
Dr AJ McKnight for BCH site and Cyril
McMaster RVH site
Equipment management co-ordinators
Biological Safety Officers
Laboratory Safety Information Update
Officer
Buildings Liaison Officers
Christine Belton
Niamh McElherron for ICSB
Cyril McMaster for IOP
Jill Kilner and Dr AJ McKnight for BCH
Fire Wardens
Please refer to table on page 70
First Aid co-ordinator
Niamh McElherron
First Aid Trained Staff
Please refer to table on page 11
2.7.17 Human Tissue Act Compliance
From 1 September 2006, all research involving human participants, their tissue or data, falls within the
regulations of HTA 2004.
To ensure compliance with this act the Centre has appointed Dr Ann McGinty as the PD for our area
and all HTA information, updates and audits are communicated and co-ordinated by Ann.
2.7.18 Infectious Agents and GMO’s
Academic staff have been appointed to advise and audit the use of Biological Materials or Infectious
Agents. Dr AJ McKnight (BCH) and Cyril McMaster (RVH).
Any proposal to work with Genetically Modified Organisms must seek approval of the NI Health and
Safety Executive.
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2.7.19 Controlled Substances/Flammable Liquids/Gases
Use of controlled substances and details of storage are audited and recorded on the CPH laboratory S
drive.
COSHH Supervisor: Christine Belton
2.7.20 Strategic Risk Register
In accordance with University regulations a register has been complied identifying all of the day-to-day
tasks and activities within the Centre and the working environment and a risk assessment has been
completed by the Chairperson of the Centre Safety Committee, Dr Jane McEneny, the CPH
Administrative Manager, Niamh McElherron, The Chief Technician, Cyril McMaster.
This register is stored and the Centre shared computer S drive and will be reviewed annually by the
above named persons.
2.7.21 Major Incident Reporting:
Major incidents are reported to the H&S Co-ordinators (Jane McEneny, Niamh McElherron and Cyril
McMaster) as soon as possible and an Incident Report form completed and forwarded to Niamh
McElherron, these are available on the S drive, ‘Documents to download’.
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2.8 Centre Health and Safety Development Plan
Health and Safety Development Plan
Centre for Public Health
1. To monitor Health and Safety Performance Indicators.
 To undertake an overview of health and safety management systems on an annual
basis.
 To ensure that the Centre’s Annual Performance Management Report is maintained
above the 90% threshold rate.
 To implement safety workplace inspections twice per year.
 To review the effectiveness of the accident reporting, monitoring and investigation
mechanisms on an annual basis.
2. To ensure fire safety standards are met in all premises occupied by CPH staff and
students.
 To continue a training programme for fire evacuation procedures with regular
announced and unannounced fire drills.
 To provide an annual health, safety and fire safety awareness session for staff.
 To continue centre-inspections relating to fire safety on a weekly basis.
3. To enhance distribution and accessibility of safety policies and information.
 To maintain the centre’s shared drive for health and safety manuals and policies.
 To continue to improve communication processes and standardised formatting for all
codes of practice and safety documentation.
4. To promote the development of a positive attitude and interest in safety amongst staff
and students, including responsibility for personal safety and the safety of others.
 To deliver annual presentations on all aspects of health and safety to staff and
students.
 To update the local protocols as and when required and disseminate such changes to
all staff and students.
 To maintain CPH health and safety information, records and audits in order to make
readily available to all staff and students.
5. To promote and develop Staff Wellbeing events and information amongst staff and
students, according to the Centre for Public Health Wellbeing policy.
 To distribute and display Wellbeing events throughout the Centre.
 To promote Wellbeing information at the annual health and safety training sessions.
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Section 3: Personnel
3.1 Nominated personnel, roles and responsibilities
Personnel responsible for Health and Safety in CPH have been nominated according to sections 2.6
and 2.7.16 of this Handbook.
Persons responsible for buildings are nominated according to section 4.9.1
3.2 Induction, training and education
All new personnel must complete the CPH induction program and all mandatory training advised in the
CPH Induction Booklet. A copy of the Booklet will be provided at Induction but is also available on the
S-Drive.
All new staff and postgraduate students must attend the appropriate QUB Staff Training and
Development Unit Courses and Health and Safety Courses as soon as practically possible. These will
have been highlighted at induction.
Please ask your Supervisor or Line Manager for details and inform our Administrative Manager, Niamh
McElherron when you attend these courses. We are required by QUB Safety Service to keep a record
for annual Health and Safety audit. Safety training should be refreshed on a regular basis.
Additional training courses can be found through Queen’s Online and also through the Staff
Training and Development Unit website.
3.2.1 Safe Use of Display Screen Equipment (DSE)
Problems may arise from the intensive use of display screen equipment.
Bad posture, for example, can lead to upper limb disorders as well as lower back ache, muscle
fatigue and eyestrain. Low humidity may lead to soreness of the eyes, while other factors may lead
to stress, for example unfavourable environmental factors and poor task planning. No matter how
good your typing skills (or lack of them), you can suffer serious ill effects if you use display screen
equipment without taking a few sensible precautions. By making full use of the equipment provided,
and adjusting it to get the best from it, you will help avoid potential health problems. Some practical
tips:
3.2.1.1 Getting Comfortable
-
-
Adjust your chair and display screen to find the most comfortable positions for your work.
As a broad guide you should adjust your chair seat height so that your forearms are approximately
level when using the keyboard. Your eyes should be at approximately the same height as the top
of the display screen.
Ensure that your lower back is well supported by adjusting the seat back height and/or angle.
Ensure you have enough workspace to take whatever documents or other equipment you need.
Try different positions of keyboard, screen and mouse to find the best arrangement for you. A
document holder may help you to avoid awkward neck and eye movements.
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-
-
Arrange your desk and display screen to avoid glare, or bright reflections on the screen. This will
be easiest if neither you nor the screen is directly facing windows or bright lights. Adjust curtains or
blinds to prevent unwanted light.
Ensure there is space under your desk to move your legs freely. Move any obstacles such as
boxes or equipment.
Avoid excess pressure from the edge of your seat on the backs of your legs and knees. A footrest
may be helpful, particularly for users of small stature.
3.2.1.2 Keyboard Use
- Adjust your keyboard to get a good keying position. A space in front of the keyboard (8-10cm from
edge of desk) is sometimes helpful for resting the hands and wrists when not keying.
- Try to keep your wrists straight when keying. Keep a soft touch on the keys and don’t overstretch
your fingers. Good keyboard technique is important.
3.2.1.3 Using a Mouse
- Position the mouse within easy reach, so it can be used with the wrist straight (have the mouse far
enough away from the edge of the desk so that the wrist is supported while you use it).
- Don’t grip the mouse too tightly and rest your fingers lightly on the buttons and do not press them
hard.
3.2.1.4 Reading the Screen
- Adjust the brightness and contrast controls on the screen to suit lighting conditions in the room.
- Make sure the screen surface is clean.
- When setting up software, choose options giving text that is large enough to read easily on your
screen, when you are sitting in a normal, comfortable working position. Select colours that are
easy on the eye (avoid red text on a blue background, or vice-versa).
- Individual characters on the screen should be sharply focused and should not flicker or move. If
they do the DSE may need servicing or adjustment.
3.2.1.5 Posture and Breaks/Work Planning
- Don’t sit in the same position for long periods. Make sure you change your posture as often as
practicable. Some movement is desirable, but avoid repeated stretching to reach things you need.
(If this happens a lot, rearrange your workstation.)
- Most jobs provide opportunities to take a break from the screen. If there are no natural breaks,
ensure that on-screen work is curtailed to 30-45 minute stints, punctuated with 5-10 minute breaks
of an alternative activity away from the DSE.
- Organise your DSE work to minimise the effects of stress. If you feel you are suffering from stress
due to your DSE work, discuss the problem with your Supervisor or Manager or the University
Occupational Health Physician.
3.2.1.6 Further Information about safe use of DSE:
CPH have members of staff that are trained DSE assessors who will be able to offer you specific advice
if you use a display screen on a regular basis.
The University Safety Service provide information about the safe use of DSE on their web site and will
also provide advice and information upon request.
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3.2.1.7 Portable display screen equipment
Users of portable DSE such as laptops, notebooks, and PDA’s are advised to read the guidance note
‘Laptop/Notebook selection and use’ → ‘The Selection and Safe use of Portable Computers’ on USS
website. The DSE assessor cannot assess all locations where these devices will be used. By reading
this document, users will be equipped to assess their own location.
3.2.1.8 Provision of eyesight test for DSE Users. Do you wear spectacles?
The rules that apply to the provision of the eyesight test and glasses (if prescribed) specifically for work
with display screen equipment can be found on the University Safety Service website.
If you have to wear prescription spectacles at work, there are forms of eye protection which can be worn
quite comfortably over them which are suitable for occasional use.
However, the cost of prescription safety spectacles and the associated eye test for those who
need them, will be met biennially by the safety office. A letter from your supervisor is required in the
first instance. Alternatively, it is possible to have your spectacles fitted with safety lenses which have
been specially made to the same prescription as your existing corrective lenses. These do not give the
same all round protection however. Ask your optician about this.
3.2.2. Data handling
3.2.2.1 Data Protection Act
QUB is required to comply with the Data Protection Act, 1998. The QUB data protection co-ordinator
has provided specific information related to the 1998 Act and local policies. The university policy can be
located via QUB Personnel webpage and selecting A-Z Information
Where digital storage of data occurs a specific advice page is provided by QUB
http://www.qub.ac.uk/directorates/InformationServices/Services/Security/FileStore/Filetoupload,294890,
en.pdf
To summarise:
 We are all obliged to keep the least personal information we need, for as short a period
possible, in as few places as are practicable, in as secure an environment as we can
reasonably manage, and in a discrete enough form that should the other obligations fail the
data would be undecipherable.
 Data includes not only patient details which may cause harm or distress if released but also
staff and student details (e.g. salary or exam marks) and commercially sensitive information.
3.2.2.2 Lab samples
Samples arriving in the lab must be anonymised (a number is preferred: patient initials or NI number
etc should not be used). Only the PI should store the name and code key [preferably one hard copy in
a locked fire-proof cabinet in a locked office/store and one digital copy in a different location but still on
site...advice/agreement from the data protection officer may be needed for removal off site].
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3.2.2.3 Lab/record books
Patient information and results from analysis entered in record books should relate only to the
anonymised code. The information contained in the record book is confidential (since it can be decoded
with the name and code key): so keep it locked up. [Keep a separate lab book.]
3.2.2.4. On-Site Digital analysis & storage




Use secure network storage (e.g. your Novell home or shared office drive)
Active computer terminals/portable computers/devices should not be left unattended
Use password protection and time-out protection
If you need to make sensitive information available use Sharepoint.
3.2.2.5. Off-Site Digital storage




Ensure authorisation is in place before University information assets (especially confidential or
sensitive data) are removed off site
Confidential data on a mobile device must be encrypted – the approved package is TrueCrypt
Memory sticks must use the University approved fully encrypted product Iron Key
Sensitive data held on a mobile device must be securely erased immediately after use
3.2.2.6. E-Mail

Do not transfer unencrypted sensitive data outside QUB; use internal email with caution
3.2.2.7. Backing up sensitive information
[NB: Where we fall down most is in the number of copies of confidential information we keep…
Computers crash and buildings burn down… Troubleshoot your information storage plans with your
supervisor or line manager: It is seldom necessary to keep as many as four copies of any data set but is
useless to keep three copies which are accessible only in one building].
3.2.2.8 Reporting a security breach
Report immediately the loss of any device containing sensitive information or any other breach of
security involving sensitive information.
If, having read the principles above, you have any concerns about your existing mechanisms for storing
and accessing sensitive information or need advice about future plans, please contact your line
manager, Information Services or your local computing support officer.
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3.3 Staff Health and Wellbeing
3.3.1Staff Wellbeing
Queen’s is committed to supporting staff wellbeing and this is demonstrated through a host of existing
initiatives, benefits and policies, all of which can be viewed on the new Wellbeing Website. Centre for
Public Health have appointed a Wellbeing Champion, Dr Helen McAneney, h.mcaneney@qub.ac.uk
Occupational Stress: Stress is not a new phenomenon, it is recognised by the University as a
contributory cause of many illnesses. The University is fully committed to the well-being of all staff. It
fulfils its responsibilities by providing a safe and healthy working environment, and to this end a range
of policies and procedures have been developed and implemented.
Please see University Stress Policy, Appendix D.
We also recommend contacting the Confidential Helpline
Staff: For work and personal problems help is at hand 24 hours a day 7 days a week with
CARECALL.
Tel: 0800 389 5362
Students: For confidential support please contact:
Email: counsellor@qub.ac.uk
Tel: 028 90 972774 (9am – 5pm Mon – Fri)
Tel: 0808 808 8000 (24 hour LIFELINE)
3.3.2 Pregnancy/New and Expectant Mothers: If you are pregnant or suspect you are pregnant it is
important that you read the university’s policy on new and expectant mothers.
Definition: A New or Expectant Mother is a member of staff or a student who is pregnant, has given
birth within the previous six months or is breast feeding. The University is committed to protecting the
health of the New or Expectant mother. All new and expectant mothers are likely to experience
general fatigue and discomfort associated with pregnancy and breast feeding. Risks to be considered
in low risk environments include manual handling, use of computer equipment, slips and falls, lone
working, stress, personal safety and other general issues.
Further information is available via the following link and Appendix H.
http://www.qub.ac.uk/directorates/HumanResources/OccupationalHealthandSafety/NewandExpectant
Mothers/
3.3.3 Tidiness and Hygiene in the Workplace: Untidiness causes accidents and can also have an
indirect effect on morale and standards of work. In addition, untidy offices and laboratories lead to
confusion for cleaning staff causing them to take risks that they may be unaware of. Floors and
pathways must be kept clear at all times and must not be used as storage areas. The University
operates clear desk policy.
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3.3.4 First Aid Provision: A list of first aid trained staff is posted on each corridor.
First Aid boxes/bags are located in the administration office in ICSB, Mulhouse and in all CPH
laboratories. An additional First Aid Action Plan is located in each laboratory beside the Spill kits at the
laboratory entrances.
3.3.5 Smoking: Smoking is not allowed in any university building. Never smoke in any stairwell or at
the entrance to any building. Please see appendix F.
BCH Site only: designated smoking shelters are provided for your comfort at the front of the Tower
Block and at the loading bay exit.
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Section 4: Premises and Environment
4.1 Areas covered by this manual
The CPH Health & Safety Handbook covers the following laboratories and offices occupied by
members of CPH:




CPH Laboratories and offices, Institute of Pathology Building, RVH
Nephrology Research Laboratories and Offices, ‘A’ Floor, BCH
Clinical Research Facility, U Floor, BCH
Institute of Clinical Science, Block B, RVH
4.2 Assessment that CPH laboratories are fit for purpose




External assessment: MHRA compliant
University assessment: Annual audit compliant
Internal assessment: Planned and unplanned inspections compliant
HAZMAT compliant
4.3 Access and Security
4.3.1 Buildings access and security
CPH laboratories and offices are restricted access only. Swipe cards are available as explained in the
Induction Booklet. IMPORTANT! → keep restricted access codes confidential. Keys and security
codes must be kept securely and any breach of security (e.g. lost keys or access cards) must be
reported immediately to the responsible person:
RVH site: Niamh / Cyril and QUB security.
BCH site A-Floor: Jill / AJ and BCH security.
BCH Site U-Floor: Roisin Martin.
Last person to leave must:



Turn off all lights
Re-set the Intruder Alarm if necessary (RVH site only)
Think about your exit route if working late and contact security if you notice any strangers:
-
RVH Security: Ext: 33553 (028 9063 3553)
BCH Security: Ext: 42000 (028 9504 2000)
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4.3.2 Using the lift




Estates will arrange the inspection and testing of the lifts.
The maximum carrying load of the lift must never be exceeded
If the lift stops between floors, press the alarm bell and wait to be released. Do not attempt to
get out on your own
NEVER use the lift in the event of a fire
RVH Site Only - Important! Do not use lift out of normal working hours. If the lift alarm is heard,
report this to Cyril McMaster (Tel 32696/32583) or QUB helpdesk on 028 9097 5152 during office
hours.
Outside office hours contact QUB security on 028 9097 5099.
BCH Site Only – Important! There are separate passenger and service lifts. The service lifts are
located beside the loading bay. Only transport goods in the service lifts. Never use the passenger lifts
to transport chemicals or any other hazardous substance.
Flammable liquids and hazardous chemicals, in particular solid carbon dioxide and cryogenic
liquids, should only be transported in passenger or service lifts in accordance with local
procedures. Please see Risk Assessments for Asphyxiant Gases on the S drive
4.3.3 Personal Security
Many areas within and around the buildings are covered by CCTV. Telephone calls may be monitored.
Advice about your personal security is available at the following link:
http://www.qub.ac.uk/directorates/EstatesDirectorate/HowDoI/Ensurepersonalsafety/
4.3.4 Bicycle Security Advice
The following advice has been issued by Safety Services:
Bicycles are a popular target with thieves because they can easily be sold. Most bicycle thefts are due
to poor quality locks that are easily cut using bolt cutters.
To ensure your bike remains safe and secure, here are a few tips:






Always lock your bicycle, even if you are just leaving it for a couple of minutes
Use designated cycle parking facilities at all times
IMPORTANT! - If using the bike shed outside ISCB, please remember to recheck the bike
shed door is closed each time you leave (sometimes the closing mechanism does not engage
fully).
When buying a bike, budget for security. The best types of bicycle locks are made of loop solid
metal (D Lock). Approved locks at a discounted rate are currently on sale at the PEC and
Estates reception
Look for products that have been tested against attack
Check out www.soldsecure.com for certified locks.
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
Students and staff are encouraged to report any suspicious activity to security on 028 9097
5099/5098. Further information is also available at:
http://www.qub.ac.uk/sites/CyclePlus/Security
4.3.5 Security in the car - advice
Please be vigilant (especially RVH site) at all times, particularly when it is
dark and it is difficult to see people lurking around. As soon as you enter your car, place your
belongings in the foot well behind the driver's seat and lock your car before you drive off.
4.4 Main Hazards identified in CPH
The main hazards in CPH have been identified by completion of the Strategic Risk Register (section
2.7.20). The strategic risk register looks at the hazards involved in certain tasks and the impact that
will arise if the control measures fail.
The main hazards include working with hazardous chemicals, using equipment, manual handling etc.
Control measures have been put in place to ensure that as far as possible, the risks are minimised.
Each member of staff or student should familiarise themselves with the strategic risk assessment for
CPH and all must read/sign any associated Risk assessments and complete any relevant training
before starting the hazardous work. University Safety Services offer training courses covering many of
the major hazards.
4.5 Fire Fighting equipment
Fire fighting equipment is provided and regularly checked and maintained. Fire extinguishers are
colour coded (see section 8). The University regularly run courses on the safe and correct use of fire
extinguishers and you are recommended to attend. Only attempt to tackle a fire if you are confident in
the correct choice of fire extinguisher, there is a second person present to ensure the escape route
does not become smoke filled and the flames of the fire are not too high.
Fire fighting equipment available for use includes: fire extinguishers, fire blankets and hoses (see
section 8).
Important!
 Fire fighting equipment (including the fire alarm system, fire extinguishers etc.) must
never be tampered with. Report any tampering immediately to the Fire Warden.
 Fire extinguishers should never be used to prop open doors!
 Never prop open fire doors – in the event of a fire the escape route could become
smoke filled!
 Escape routes (corridors, stairs etc.) must never be obstructed with e.g. clutter.
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4.6 Safety and Security while Lone working
4.6.1 CPH Lone-Working Policy on Working outside of Normal Hours
It is recognised that on occasions some members of staff and students may either choose to or be
required to work alone outside normal hours in offices and laboratories. The Centre for Public Health
has put the following regulations in place to ensure the health, safety and personal security interests of
such individuals.
Definition of Working in Isolation
Any person who is not working as a member of a group or is not working under close supervision shall
be considered to be working in isolation. A lone worker shall not undertake or be permitted or
instructed to undertake any hazardous work or engage in any hazardous work activity particularly
outside of normal hours.
Any individual who is planning to work alone in a laboratory or office outside the hours of Monday
to Friday 7am until 7pm must in advance of such work report to their Line Manager and inform
them of the following information:
o
o
o
o
DATE and TIME when they must work alone;
PLACE or PLACES where the work will be carried out;
IF ANY SPECIAL ARRANGEMENTS are required and how these will be provided.
Your line manager will complete an ‘Permit to Work Out of Hours’ which must be
carried at all times as QUB security may request to see this written permission.
4.6.2 Permit to work out of hours form:
The correct details must be entered and the form signed before any lone working takes place.
(This form can be downloaded from the Documents to Download folder on the S shared computer drive):
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Permit to Work Out of Hours
Centre for Public Health
Queen's University Belfast
Institute of Clinical Science,
Block B
Grosvenor Road
Belfast
BT12 6BJ
Tel. 028 906 32608
Date:
TO WHOM IT MAY CONCERN
Permission to work outside of normal working hours (7.00am - 7.00pm) weekdays and all
times at weekends and holidays, in <state all appropriate locations >, has been granted to
< Staff or Students name >.
This permission is granted until < Sate Period of Permission>,unless employment or
graduate student status terminates before this date, in which case this permit must be
returned to the undersigned..
All laboratory work during this period must be in accordance with Lone Working Practices
stated in the Centre for Public Health Induction Handbook and comply with the CPH Lone
Working Risk Assessments for Offices and Laboratories.
Please note: High risk activities identified by COSHH must not be performed outside
normal working hours.
Yours Sincerely
Line Manager/Supervisor
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4.6.3 Additional Lone working Arrangements

Late working, between the hours of 7pm and 11pm in offices is generally permitted. Where staff
in a building do not normally work late, they must notify QUB Security Tel. 90 975099 RVH
only.

A RISK ASSESSMENT for the laboratories and offices will be used to identify any reasonably
foreseeable hazards and the risk of actual harm arising from such hazards. Arrangements will be
put into place to eliminate or minimise any risks. The Risk Assessment MUST be read before
lone working commences.

A Lone- Working, Out of Hours LOG is located on a shelf in the foyers of the Pathology Building
and ICSB. This must be completed on the day of lone working before work commencement
and on leaving.

It is HIGHLY RECOMMENDED that the lone worker contacts their line manager/work colleague by
telephone or text message to inform them of when they enter University premises to begin work
and when they leave University premises on work completion.

Trainee staff and 1st year students must not work in the Nephrology Research Labs (BCH Site)
outside working hours unless fully supervised. Non QUB or BHSCT staff are not allowed in the
Department outside working hours unless accompanied by a member of staff.
Please see the following link for further information:
http://www.qub.ac.uk/directorates/HumanResources/OccupationalHealthandSafety/GuidanceNotes
/LoneWorking/
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4.6.4 Lone working Risk Assessment
All workers in Centre for Public Health must read the Lone Working Risk Assessment that
applies to both Laboratories and Offices:
Hazard
Risk
Control Measures
Work Pattern
Working alone
Low/Medium/High
Workplace/Process:
Slips, trips and falls
Low
Ensure the following: trailing cables and unnecessary
items on lab and office floors are tidied away; spillages
are cleaned up and items are stored below shoulder level.
DO NOT work alone if your mobility is impaired.
Fire or other emergency
Medium
Ensure that you have received fire safety training and that
you are familiar with the emergency procedures for your
area. Ensure fire doors are kept shut. Emergency
numbers are located by the telephones.
Electrical Accident
Low
Ensure that all equipment is PAT tested and have been
labelled “Pass.” Inspect electrical cables regularly for
signs of damage. Do not use defective equipment. Don’t
interfere with plugs, cables etc when item is connected to
the power supply.
Chemical Hazards
Low/Medium/High
No high-risk work to be done out of hours. See relevant
COSHH forms for your work in blue lab folders or on the
lab computers shared drive. Wear the recommended
protective clothing and equipment. Chemicals must be
kept in original containers and in appropriate locations.
Solutions should be clearly labelled – including hazard.
Flammable chemicals must never be used near heat
sources. Stock bottle must be transported using an
enclosed bottle carrier. Disposal must be via the correct
method.
Biological Hazards
Low/Medium/High
See relevant risk assessments and SOP’s for the
designated lab.
Radiation Hazards
Low/Medium/High
Radiation work for out of hours lone workers is prohibited.
The line manager must be informed and a signed
‘Permit to work out of hours’ form completed before
any out of hours work is undertaken. Lone and out of
hours working should always be avoided where possible.
Work should be re-scheduled to a time when other staff
and students are present. Where lone working is
unavoidable the out of hours log book must be
signed.
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Equipment:
Laboratory equipment
Low
Only use equipment after being instructed how to do so by
a competent person and when your competence has been
demonstrated and approved. Visual inspection should be
made to ensure there is no damage or defects. A PAT
label should be present. When switched on, check for any
warning indicators (lights, alarms, gauges). If any faults
are found the equipment should be switched off and
reported to the chief technician/floor manager’s a.s.a.p. All
equipment must be used and maintained in accordance
with the manufacturer’s instructions. For high/low
temperature equipment the appropriate personal
protective equipment must be worn (e.g. insulated
gloves). Equipment must be switched off when not in use
and at the end of the day.
Potentially hazardous
equipment
Medium
This will not be permitted. Re-schedule work to a time
when other members of staff are present.
Manual handling of loads
Medium
This will not be permitted. Re-schedule work to a time
when assistance is available.
Low
Outer IOP doors are electronically locked. Access control
points – swipe card and keypad access for authorised
persons to enter building. Intruder alarm system installed
on LGF and is linked to QUB security. Two personal
alarms for lone workers are located at either end of the
LGF corridor. Ensure that you know how to contact QUB
and RVH/BCH security by telephone promptly.
Emergency numbers are posted by telephones. Do not
confront the intruder, lock your door and await the
arrival of Security. In an emergency dial 9999 and
33553 (RVH) or 42000 (BCH).
Individual
assessment
Ensure that any medical conditions which might be
relevant to you working alone are fully discussed with your
line manager and if necessary, Occupational Health (Tel:
90 975520) and your own GP. Do not work alone if any
such condition is assessed as putting you at increased
risk.
Personal Safety:
Intruder in the building
with potential for
verbal/physical assault to
staff/students
Individual:
Personal medical
conditions etc.
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4.7 Notices, symbols and warning signs
Biohazard
White coats
No smoking
Radiation
Fire exit
No Mobile phones
Compressed gas
First Aid
Dry Ice
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Chemical hazard symbols http://www.hse.gov.uk/
Symbol
Abbreviation
Hazard
Description of hazard
Hazard Symbols - Physicochemical
E
explosive
Chemicals that explode.
O
oxidising
F+
Extremely
flammable
F
Highly flammable
Chemicals that react exothermically with
other chemicals.
Chemicals that have an extremely low flash
point and boiling point, and gases that catch
fire in contact with air.
Chemicals that may catch fire in contact with
air, only need brief contact with an ignition
source, have a very low flash point or evolve
highly flammable gases in contact with water.
Hazard symbols - Health
T+
Very toxic
T
toxic
Carc Cat 1
Xn
Category1
carcinogens
category2
carcinogens
category3
carcinogens
category
1
mutagens
category
2
mutagens
category
3
mutagens
category
1
reproductive toxins
category
2
reproductive toxins
category
3
reproductive toxins
harmful
C
corrosive
Xi
irritant
Carc Cat 2
Carc Cat 3
Muta Cat 1
Muta Cat 2
Muta Cat 3
Repr Cat 1
Repr Cat 2
Repr Cat 3
Chemicals that at very low levels cause
damage to health.
Chemicals that at low levels cause damage
to health.
Chemicals that may
increase its incidence.
cause
cancer
or
Chemicals that induce heritable genetic
defects or increase their incidence.
Chemicals that produce or increase the
incidence of non-heritable effects in progeny
and/or an impairment in reproductive
functions or capacity.
Chemicals that may cause damage to health.
Chemicals that may destroy living tissue on
contact.
Chemicals that may cause inflammation to
the skin or other mucous membranes.
Hazard symbols - Environmental
N
dangerous for the
environment
Chemicals that may present an immediate or
delayed danger to one or more components
of the environment
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Globally Harmonized System of Classification and Labelling of Chemicals (GHS):
1.
2.
6.
1.
2.
3.
4.
5.
6.
7.
8.
9.
3.
7.
4.
8.
5.
9.
Explosive
Flammable
Oxidising
Gas under pressure
Corrosive
Toxic
Caution – indicates less serious health hazards e.g. skin irritation
Longer term health hazards e.g. carcinogenicity, respiratory sensitisation etc.
Dangerous to the environment
4.8 Local Rules for visitors, cleaning staff and service engineers
All staff and students must take reasonable care that their actions or emissions can not
in any way affect the health of cleaning staff. Local Health and Safety Rules exist for
the safety of domestic staff, service engineers and visitors.
RVH site: these can be found on the S drive (see Appended Documents 1 – 3).
BCH site: these should be obtained from a senior member of staff in the Regional
Genetics Centre.


It is your responsibility to ensure your work area is safe for all visiting persons as
well as for yourself. Visitors must be accompanied at all times.
Equipment must be decontaminated in advance of service engineers visits and a
decontamination certificate must be issued. In addition, a Permit-to-work is
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required for Service engineers (section 4.11).
4.9 Monitoring, control and recording of environmental conditions
4.9.1 Reporting of Faults
All issues relating to plumbing, heating, lighting, power supply, blocked toilets, leaks etc
should be reported by email to the appropriate Buildings Liaison Officers (BLO). Please
include the room number and a brief description of the problem in your e-mail.
Buildings Liaison
Officer
Niamh McElherron
Building
Institute of Clinical Science Block B
(ICSB), RVH
Contact e-mail
n.mcelherron@qub.ac.uk
Cyril McMaster
Institute of Pathology (IOP), RVH
c.mcmaster@qub.ac.uk
Jill Kilner
Nephrology, BCH Site
j.kilner@qub.ac.uk
Dr AJ McKnight
Nephrology, BCH Site
a.j.mcknight@qub.ac.uk
Roisin Martin
CRF, BCH Site
r.martin@qub.ac.uk
On RVH site: the BLO will log a Planon request to QUB Estates Department.
On BCH site (Nephrology): If possible, the Regional Genetics Operational Managers
(Judith Briggs, Borghert Borghmans (BJ)) must be informed beforehand. Otherwise they
must be told as soon as possible. Make sure to record the job number.
 During working hours (Monday – Thursday 8.00am to 4.30pm, Friday 8am –
1.30pm): call the faults reporting and enquiries line – 028 950 40377.
 Outside working hours, for emergencies only: phone the operator ‘0’ and ask for
the duty engineer.
4.9.2 Noise in the Workplace
If a noise problem exists, an investigation by the University Safety Officer will be
arranged and measurements taken if necessary. Ear plugs may be used but should only
be used for short periods. Measurements have been recorded for the LC Tandem MS in
Lab C (RVH) and the Pre-PCR and Post-PCR labs (BCH). The results are recorded on
the S drive. It is always recommended to limit your exposure to irritating noises if
possible.
Radios that have been PAT tested are permitted but should be kept at a low volume,
please consider others.
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4.9.3 Removal of waste
There are specific routes of disposal for all waste generated in CPH.
4.9.3.1 Furniture, Electrical and Electronic Equipment (WEEE)
 All obsolete/surplus furniture and WEEE must be disposed of in an
environmentally friendly manner.
 DO NOT PUT ELECTRICAL WASTE INTO SKIPS OR BINS!
 Equipment (excluding computers) that may be of use elsewhere in the University
may be sold (see Purchasing Office website).
Computers: If you wish to dispose of a computer please seek permission from your
supervisor/line manager (BCH site: please inform Dr A.J.McKnight). Next, contact
the CPH Inventory Officer, who will update the CPH equipment inventory register.
IMPORTANT: DO NOT remove the inventory sticker. Finally, email the BLO who will
arrange collection and disposal according to QUB policy.
Lab equipment: Disposal of laboratory equipment should be arranged via the Chief
Technician. It is the users responsibility to ensure that all items for disposal are
appropriately cleaned. If equipment has been used in the lab (or other potentially
hazardous setting), it must be decontaminated and certified safe before collection.
Contact the CPH Inventory Officer, who will update the CPH equipment inventory
register. IMPORTANT: DO NOT remove the inventory sticker. Certain items of
equipment e.g. MJ Tetrad PCR machines may be returned to the manufacturer for
disposal.
Other brown and white electrical equipment: All other obsolete/surplus furniture
or electrical equipment that is beyond economic repair, including computer monitors,
fridges, freezers, microwave ovens, etc must be disposed by e-mailing the BLO who
will contact estates and arrange collection. Please include the equipment inventory
number and room number. IMPORTANT: DO NOT remove the inventory sticker. A
minimum number of major items must be accumulated across the University before a
free collection can be arranged. Otherwise there may be a charge for disposal of
these items via the waste contractor. Examples of prices are on QUB Estates
website.
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4.9.3.2 Waste for Recycling
 Ink/ toner cartridges: should not be disposed in general waste but should be
recycled via the CPH admin office, ISCB (RVH site) or in the envelope provided
or else returned to the manufacturer (BCH site).
 Cardboard boxes: should be flat packed by staff and students and either left
beside the bin for daily collection by the domestic staff to a dedicated waste bin
(RVH) or placed into the blue dumpster in the waste disposal lift lobby (BCH). It is
not the duty of domestic staff to flat pack boxes.
 Paper: On RVH site waste paper may be recycled using the white ‘SITA’ bags
(see section 4.9.3.3). The University currently does not recycle paper from BCH
site.
 Batteries: Dispose of regular batteries into QUB battery tubes in ICSA or MBC.
Lithium batteries must be given to the BLO who will arrange disposal through
Planon.
 Equipment: Obsolete equipment may be recycled through the University,
contact the Chief Technician.
4.9.3.3 Confidential Waste
All waste containing sensitive information must be shredded before disposal. This
includes information that could reveal the identity of samples, personal information about
staff and students, as well as documents for disposal that may contain purchasing card
information. White ‘SITA’ confidential waste bags are available from the Stationery
store or Laboratory Floor Managers or Chief Technician. Appropriately filled bags (do not
overfill) should be sealed and left in designated areas for collection.
4.9.3.4 Domestic waste is removed daily by the cleaning staff.
4.9.3.5 Laboratory waste is removed via the clinical and chemical waste disposal
routes described in section 15.
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4.10 Storage facilities for hazardous substances
It is imperative that hazardous substances are stored correctly. Great care must be
taken to ensure that incompatible substances are not stored together. All stored
Chemicals and chemical waste must be clearly and appropriately labelled.
The following storage facilities are available in CPH:





Locked cupboard: A locked cupboard is available underneath the fume
cupboards (RVH) for storage of hazardous, toxic chemicals at room temperature.
Locked fridge: A locked fridge is available for storage of hazardous, toxic
materials that require refrigeration.
Flammable Cabinet: All flammable substances must be stored in a locked fire
proof cabinet. HMRC request that ethanol must be kept in a separate cabinet and
usage recorded by the key holder.
Corrosive cabinet: Only corrosive chemicals should be stored in this cabinet.
Cryogenic Dewar: For storage of liquid nitrogen. These must be kept in a
suitably ventilated area.
4.11 Permit to work
There are certain activities in CPH that require a ‘Permit to Work’. This permit must be
completed before the activity is started and must be signed by both the worker and the
responsible supervisor. Copies of the forms are available from the chief technician or
floor managers.
Examples of activities that require a permit to work include:



Buildings maintenance and repair works. Normally the Permit to Work for
contractors is already in place via the estates department, but it is essential to
check that estates are aware that the worker is on site.
Servicing/ repair of equipment
e.g. fume hoods, centrifuges, pipettes,
photocopier
Lone working (section 3.6.2)
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Section 5: Equipment and Materials
5.1 Use of Fume cupboards
Before commencing work users must read the guidelines on the use of general-purpose
fume cupboards and must complete the pre-use operator check (steps 1-4 below). Also,
users should check the local exhaust ventilation report to ensure that the service and
inspection was satisfactory and has not expired. The local exhaust ventilation report is
attached to the outside of the fume cupboards. Users must sign the checklist attached to
the front of the fume hood.
1. Check that the sash operates correctly and position the sash no higher than the
indicated maximum sash height.
2. Check that the airflow monitor ‘ AIR SAFE ‘ light is illuminated green and the airflow
indicator needle is in the green range.
DO NOT use the fume cupboard if:
- The airflow indicator needle is in the red range.
- The ‘ AIR FAIL ‘ light is illuminated red.
- There is an audible alarm tone.
- Any other fault is noted.
3. Check that the fume cupboard internal light functions correctly.
4. Check that other services to be used e.g. water, electricity, gas are functioning
correctly.
5. Only place the chemicals/equipment required for the procedure in the fume
cupboard. Avoid unnecessary clutter.
6. Apparatus/equipment must be set back at least 15cm from the front edge of the fume
cupboard.
7. Do not place apparatus/equipment so far back that it interferes with the bottom ‘back
baffle ‘ slot or that the user is required to place his/her head in the working chamber
to operate the equipment.
8. Do not operate electrical equipment in the fume cupboard if flammable liquids are
being used.
9. Minimise sources of high heat load e.g. naked flames, centrifuge, hot-air dryers.
These will disturb the air flow pattern and reduce the overall efficiency of
containment of the fume cupboard.
10. If large objects e.g. safety screens, trays are to be used they should be raised about
50mm off the work surface with blocks. This will help to reduce turbulence.
11. Reduce the sash to its lowest practicable height as soon as possible.
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12. While the procedure is in progress minimise movement of the sash. Any movement
of the sash must be made slowly.
13. Avoid rapid hand movements when working inside the fume cupboard.
14. Avoid rapid movements in the area adjacent to the fume cupboard.
15. Avoid leaning close to the front edge of the fume cupboard.
16. Do not leave hazardous processes in the fume cupboard unattended.
17. Clean up any spillages in the fume cupboard promptly and effectively.
18. When the process is complete, keep the sash at the lowest level for a further 10
minutes to ensure that all fumes and vapours have been cleared before raising the
sash to its maximum position.
19. If applicable, allow the glassware/apparatus to cool.
20. Dismantle the apparatus and set it aside for cleaning.
21. Tidy up the fume cupboard. Remove all equipment that is no longer needed. Return
chemicals to their proper storage place.
22. Do not use the fume cupboard as a storage area.
23. If appropriate, decontaminate and clean down the walls and worktop of the fume
cupboard chamber.
24. Lower the sash to its lowest working position.
If any faults or deficiencies in the system are noted these should immediately be brought
to the attention of the Floor Manager or Chief Technician.
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Section 6: General Health and Safety – Key points
6.1 Introduction
Before undertaking any procedure or ordering any chemical make sure you know the
hazards involved and complete a Control of substances Hazardous to Health (COSHH)
form, Standard Operating Procedure (SOP) and RISK ASSESSMENT if necessary.
SOP’s are available for all laboratory equipment and are displayed beside or on each
piece of equipment. Please fill in the associated log sheets for use on general pieces of
equipment. Laboratory Procedures (LP) for assays and experiments will be available
from your supervisor or senior technician and also on the S Drive. In addition, lab
equipment maintenance records will be retained in each lab for all relevant equipment.
Servicing of equipment will take place on a regular basis.
 Read container labels and Material Safety Data Sheets supplied with chemicals
(MSDS) and take the necessary safety precautions
 Always follow the safety procedures
 Use the protective equipment properly
 Report any hazard or defect to your supervisor or safety officer
 Know what to do in case of accidents/spills etc
 A high standard of personal hygiene is important.
 Hands must be washed thoroughly at the designated sink before leaving the lab.
 Avoid putting hands near mouth at any time.
 There must be no eating or drinking in any laboratory.
 First Aid: Find the first aid box in your laboratory and know who the nearest first
aider is and how to contact them
 Obey all safety signs and instructions and be familiar with the emergency
procedures and emergency exit routes applicable to your laboratory.
 Never run in the laboratory or along corridors
 Never indulge in horseplay in the laboratory
 Always look where you are going and exercise care when opening and closing
doors
6.2 Staff Health
Hepatitis B vaccination: All workers handling biological substances are advised to
consider vaccination against Hepatitis B before starting work. The vaccine is available
from Occupational Health or your GP and a booster is required after 5 years.
Latex gloves and the product chlorohexidine (found in various antiseptic wipes
etc.): are banned from use in CPH because of the risk of sensitisation/ anaphylactic
shock. If a research project requires use of either of these items, the Chief Technician
must be consulted and a Risk Assessment completed before they are brought on-site.
Appendix J
Repetitive tasks and RSI: Take regular breaks and stretch frequently when performing
repetitive tasks such as manual pipetting in order to minimise the risk of repetitive strain
injuries.
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Skin wounds: such as cuts, abrasions, or eczematous leisions should be protected with
waterproof dressings.
6.3 Good Housekeeping
Keep working surfaces clean and tidy – it is YOUR responsibly to keep your
working area clean.



















Do not use corridors or stairwells as storage areas.
Treat all chemicals with respect and handle them in accordance with COSHH
guidelines.
All chemicals and reagents must be properly labelled and should be returned to
their proper storage place immediately after use, with the label facing outwards.
Manufacturers’ and suppliers’ safe operating instructions must be consulted
before using equipment or materials.
Electrical cables should be tucked away to avoid slips, trips and falls.
Always switch off and unplug equipment before cleaning, adjusting or changing
parts
Protect all electrical equipment from liquid spillage.
Do not tamper with the electrical equipment unless you have been trained.
Do not use or operate any laboratory equipment without training. Always follow
the maker’s instructions.
The lab floor should be free of obstruction. Reagent bottles and apparatus left on
the floor can cause accidents.
Wet floors can lead to slipping accidents.
Any spillage of chemical on the bench or floor should be cleaned up in
accordance with laboratory procedures and disposed of safely.
Fire-fighting equipment should not be tampered with. Do not wedge open fire
doors.
Do not leave sharps on a working surface or loose in a drawer. Dispose of
immediately in specially marked sharps containers.
Gas cylinders must be supported with a safety chain.
Heavy items must be stored below shoulder height.
No ladder should be climbed without it being steadied at the bottom by another
person.
Chemical and Clinical waste; Local rules apply. Waste chemicals should be
disposed of in accordance with approved procedures.
When leaving the laboratory check your work area for tidiness, check for
any potential hazards to others, and wash your hands thoroughly.
Tidiness and Inspections
Please ensure that working areas remain tidy at all times and that chemicals, solutions,
samples etc are returned to their proper place once your lab work is completed. Please
wipe down benches after work with Trigene (RVH) or Microsol 3+ decontaminant (BCH).
and clean any centrifuge spills. Untidiness causes accidents. Keep floors and exit
pathways clear.
Regular inspections will be carried out by the Floor Managers and problem areas
highlighted.
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6.4 Hygiene for laboratory users
6.4.1 Personal belongings: Before entering the laboratory place personal belongings
such as bags, coats, sweatshirts etc. into the lockers provided (RVH site) or leave in the
secure office (BCH site). NB: outdoor coats and cardigans must not be hung on coat
hooks in the lab corridor which are for lab coats only!
6.4.2 Keep objects away from the mouth: Nothing should enter the mouth while in the
laboratory. Take care to avoid any contact between your fingers and mouth while in the
lab, such as biting finger-nails. Likewise chewing pens or pencils, biting of sellotape and
licking of envelopes should always be avoided in the lab.
Mouth pipetting is not permitted in CPH → a pipetting aid should always be used.
6.4.3 Hand Washing: Designated hand washing sinks and soap are available in
laboratory areas. Wash your hands regularly when working with chemical or biological
agents, and must be washed thoroughly before leaving the lab, especially before meals
or snacks, and before visits to the toilet.
6.4.4 Food and drink: Food and drink must never be stored, prepared or consumed in
laboratories or chemical storerooms. On BCH site, food and drink is not permitted to be
taken through the swipe card access lab area. This includes bottles of water, chewing
gum etc.
6.4.5 Smoking: Smoking is not allowed in laboratories or in any university building.
Never smoke in any stairwell or at the entrance porch to any building. Please see
appendix F. BCH Site only: smoking shelters are provided for your comfort at the front
of the Tower Block and at the loading bay exit.
6.4.6 Cosmetics: Do not apply cosmetics in the laboratory. Do not put the hands up to
the face or eyes while working in the lab, especially while wearing gloves.
6.4.7 Mobile phone use is restricted in some laboratories. If wearing gloves, remove
before answering to avoid cross-contamination.
6.5 Personal Protective Clothing and Equipment
6.5.1 Laboratory Coats
Laboratory coats are for the protection of your person and your clothing from
contamination and chemicals.
 Important! Laboratory coats provided must be worn in the CPH laboratories.
 They must always be fastened up.
 Lab coats must not be worn outside the lab area (e.g. into offices or tearooms)
unless disposing of waste or transporting chemicals.
 Lab coats should be regularly laundered and kept in good repair. A laundry
service is provided (lab coats must not be taken home for washing).
 Contaminated coats should not be worn.
 Specific coats are available and must be worn for Tissue Culture and Illumina.
Please refer to the Laboratory Procedure (LP) for details.
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6.5.2 Protective gloves and glove choice

Wear suitable disposable gloves and dispose of appropriately.

Disposable nitrile gloves are single use only. Remove worn gloves carefully by
gently turning inside out whilst peeling of the hand so that any contamination is kept
inside the glove at disposal. Discard as soon as removed – DO NOT keep gloves in
lab coat pockets or leave on the bench.

During research procedures gloves may become contaminated with chemical or
biological material. Always change contaminated gloves immediately, before
handling any lab equipment or furniture.

Never use gloves that are defective e.g. have a hole in them. Always change
defective/ damaged gloves immediately. If defective gloves are not a disposable
variety, inform the Floor Manager of the defect.

Never wear gloves into public areas as this may lead to cross-contamination





Never use a telephone while wearing gloves
No gloves on door handles at any time (including laboratory doors!)
Never operate lifts while wearing gloves
Remove gloves before using the lab computers and when using certain
equipment as instructed in the LP.
Disposable latex gloves are not permitted. Appendix J
Always choose appropriate gloves for the task. Gloves available and when to
wear them are indicated below (the style or colour may vary depending on the
supplier):
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Glove type
Image
When to use
For most chemical work in CPH, disposable
nitrile gloves will be the best choice.
Disposable Nitrile
NB: some chemicals can quickly penetrate
or damage a nitrile glove. Double gloves may
be required → check the risk assessment!
Vinyl
Usually adequate if you need protection
against grime or infection, or if you are using
gloves to protect the material you are
handling e.g. clean room work / tissue
culture.
Cryogenic
Must be worn when handling cold liquids e.g.
liquid nitrogen. Also when working in the
Ultralow temperature freezers or when
handling dry ice during packing or unpacking
of deliveries / shipments.
Heavy nitrile
Must be worn for cleaning up certain
chemical spills when indicated on the
COSHH form
Heat proof
Must be used when handling hot items e.g.
removing items from the autoclave or
microwave oven
Barrier chemical
resistant
Must be worn for cleaning up certain
chemical spills when indicated on the
COSHH form
Cotton liners
Available for use by anyone with eczema or
other skin condition → are worn underneath
the other glove types to protect the skin.
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6.5.3 Eye and face protection
Protect your eyes and face:
 Safety spectacles and / or goggles are located in each lab
 Full face visors are provided
 A special full-face visor is required when working with un-shielded UV light.
 Know the location of eye wash stations and fire extinguishers in your work area
Safety spectacles
goggles
visor
Eye protection:
There are many processes and operations which are considered to involve a special
risk of injury to the eyes. If you are employed in any of these processes or operations
your employer will provide you with suitable protective equipment, and you must wear it.
This is a legal requirement and is designed to protect you. It may be that your job is
such that your employer feels justified, in your interests, to provide eye protection, even
if he is not required by law to do so. In these circumstances, your co-operation is
essential.
You must wear eye protection:
• When handling any dangerous liquid
• When working in dusty conditions
• On any other occasion when it is wise to do so.
Taking care of your safety glasses:
1. Badly fitting eye protectors are not only uncomfortable, but they may not adequately
protect your eyes. Make sure that new protectors fit properly and have them
readjusted periodically.
2. The framework of your protectors must be in good condition if they are to stay in
place on your face. Avoid ‘do-it-yourself' repairs; damage must be repaired by
experts.
3. Toughened glass lenses. Damage such as pitting or scratches on the surface of
these lenses can reduce their protective quality. Toughened lenses can ' wear out ' !
4. Plastic lenses. These are very easily scratched and damaged. Never lay them down
on their lenses. This applies also to any other type of lenses.
5. Prescription spectacles. If you wear corrective spectacles with safety lenses, ensure
that the lenses are always to your correct prescription. This means regular eye
examinations.
6. Finally, keep your eye protection clean and protect it from damage when not in use.
Report any loss or damage without delay.
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If prescription safety spectacles are required, read section 3.2.1.8
6.5.4 Respiratory protection: Protect your airways. Inhalation of any chemical or
biological dust should be avoided. Read the COSHH guidelines. Fume cupboard, dust
masks, and respirators are all available and must be used when advised on the COSHH
Risk Assessment. Asthma sufferers, please see appendix C.
A respirator is located on the windowsill in Lab A (LGF 017) beside the fume cupboard.
Ask the Floor Manger or Chief Technician as they have been ‘face-fit’ tested and trained.
6.5.5 Footwear: Ensure that your foot ware is suitable, open toed shoes or sandals
offer no protection against injury. BCH site only - open toed shoes or sandals are not
permitted and blue shoe covers provided must be worn by anyone with unsuitable
footwear.
6.5.6 Ear protection: Protect your ears. Avoid prolonged exposure to excessive
noise as this can result in permanent damage to hearing or wear ear protection (ear
plugs should only be worn for short term use).
6.6 Safety shower: A safety shower is available in each lab for use in emergencies.
Make sure you know the location and how the shower operates.
6.7 Hair and jewellery: Long hair, jewellery, beards, ties and other items hanging
from your person can be hazardous in the laboratory. Hanging items must be removed
or firmly secured before entering the laboratory. This simple measure will help to ensure
that:
 personal items do not trail on the bench and become contaminated while working
in the lab
 experiments (often expensive and time consuming) do not become contaminated
by trailing objects
6.8 Using DSE in the lab: Read the safety guidance in Section 3
Always follow the laboratory procedure when using computers and software programs in
the lab. BCH Site: some lab computers are glove free zones – please take care to
remove gloves before using these computers. Do not remain in the lab to analyse data
on a computer if the software required for analysis is available in the office.
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6.9 Working with hazardous chemicals
6.9.1
Chemical Hazard warning symbols in common use
Flammable
Explosive
6.9.2
Corrosive
Oxidising
Toxic
Highly flammable
Irritant
Environmental hazard
Labelling of containers
IMPORTANT! → All chemicals, diluted chemicals, solutions and wastes must be labelled
appropriately. It is the responsibility of the researcher to correctly label their containers.
Sloppy labelling of hazardous chemicals and diluted chemicals is seen as a serious
health and safety breach.
 When opening a new chemical/solution, it should be dated and initialled by the
user with a black permanent marker pen.
 When making up a solution in the lab, this should be labelled using a black
permanent marker pen. Pre-printed labels will be available with the following
information:
SOLUTION NAME:
CONCENTRATION & pH (if applicable):
USER NAME:
DATE MADE:
EXPIRY DATE:
HAZARD WARNING (if applicable):
6.9.3
Labelling your research
All research experiments, equipment etc using any form of hazardous material must be
carefully and clearly labelled. It is the responsibility of the researcher to correctly label
their experiments etc, sloppy labelling is seen as a serious health and safety breach.
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6.9.4
Handling of Chemicals
Carefully open all chemical containers. Many chemicals can become pressurised or
produce hazardous vapours upon storage. Dispense carefully to minimise risks from
inhalation and contact injuries. Some chemicals must be kept in specialised
atmospheres to ensure the purity and to avoid unwanted reactions. Know the properties
of the chemicals you are using and buying.
No reagents or chemicals should touch the designated hand washing sink
6.9.5
Flammable solvents
Solvent vapours can be ignited by naked flames, the electric elements of heating
mantles, hotplates, sparks from thermostat controls, switches, the hot glass surface of
electric light bulbs and other surfaces at or above 100 C.
Winchesters of flammable solvents must be stored in the storage area under fume
cupboards or other designated storage bins. IMPORTANT! Storage space is restricted.
Ensure that Winchesters can be correctly stored before placing an order!
Winchesters or containers of solvents must not be kept or used on benches, use the
fume cupboards.
Only small volumes of solvents <500mls, in closed containers, may be used outside the
fume cupboards for day-to-day activities.
6.9.6
Highly reactive substances
Some chemicals are highly reactive in specific circumstances. For example alkali metals
when used near water or any type of wet surface extreme care should be taken because
alkali metals react violently with water.
6.9.7 Dilution of Concentrated Acids:
Most people carry out this procedure without thinking. Concentrated acid must always be
added to water (or the lesser concentrated solution)
IMPORTANT! → NEVER add water to concentrated acid:
Adequate ventilation must be provided for particularly smelly or noxious procedures. All
hazardous chemicals must be clearly labelled, especially when taken from original
containers and placed in reagent bottles or other containers.
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6.9.8
















Working with carcinogens
All known carcinogenic substances must be labelled as such.
All known carcinogenic substances must be kept in a closed container in a locked
cupboard, freezer or refrigerator.
All persons intending to use known carcinogens must be aware of the risks, and
be fully trained in the handling of them.
All inhalation, ingestion or skin contamination must be avoided.
All work with known carcinogens must be carried out in a fume cupboard,
suitable safety cabinet or isolated area.
Benches should be covered with disposable material.
Suitable gloves must be worn. Goggles may need to be worn.
Any part of the body contacted by a known carcinogen must be washed in cold
water for five minutes.
Lab coats must be worn.
Contaminated lab coat should be disposed into purple top bin!!
Dispose gloves into purple top bin.
Carcinogenic solutions must be used in a tray to contain spills.
All utensils and containers must be thoroughly washed in cold water after use.
All other waste must be sealed in a polythene bag and incinerated.
No eating, drinking, smoking, biting of fingernails or application of cosmetics is
allowed in laboratories where known carcinogens are used.
For specific information on working with carcinogens please see Appendix I
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6.10 Working in tissue culture
1. Avoid entering Tissue Culture unless intending to work with or examine cells.
2. If hood or cabinet use is required, please pre-book using the sheet displayed on the
door of Tissue Culture. Remember to place reagents to warm 30 minutes prior to
commencement of booking time.
3. Remove lab coat before entering Tissue Culture. A separate clean labelled lab coat
should be used for Tissue Culture only and should be kept inside Tissue Culture.
4. Preparation is critical. Obtain any reagents, materials, etc. which are required for
your procedures and are not stored in Tissue Culture before entering Tissue Culture
as this will save the time taken to remove coat and gloves every time you have to
leave.
5. Warm reagents, media etc. in 37ºC water bath prior to use for at least 30 minutes.
Do not leave bottles in incubators or on lab benches to warm. When not in use, store
reagents in refrigerator or cupboards as appropriate.
6. Wear gloves before opening incubators or handling flasks/plates containing cells.
Only open incubators when absolutely necessary, and for as brief a time as possible.
Avoid disturbing flasks/plates belonging to others. Periodically check incubator
temperature and CO2 levels and report any discrepancies.
7. When using Class II cabinet, wipe all reagent bottles, pipette aids, micropipettes, tip
boxes etc. with 70% alcohol prior to placing in cabinet. If any liquids are spilled in the
cabinet, wipe up immediately with tissue and swab surface with 70% alcohol. If you
leave the cabinet temporarily, wipe gloves with alcohol before returning.
8. When work in Class II cabinet is completed, ensure that all your reagents are
removed and correctly stored. Only sterile glass Pasteurs, pipette tips and a tube
rack should remain in cabinets. Swab cabinet surface thoroughly with 70% alcohol
before switching off. Place a little alcohol in suction tube and run pump for a few
seconds to sterilise tubing.
9. All used glass Pasteurs, scalpels, needles and pipette tips should be placed in
sharps box. Empty glass bottles should be capped and placed in basket provided.
10. Hazardous waste should be placed in bags provided. Do not fill more than ¾ full
before changing. Staple top 5-6 times and leave for autoclaving.
11. Non-hazardous waste such as packaging, blue tissue, gloves etc. should be placed
in blue/black waste bags. Do not place in autoclave bags.
Ensure microscope is switched off before leaving Tissue Culture.
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6.12
Electrical Safety

Only electrical equipment that has been PAT tested and found to be safe can be
used in CPH. This includes personal items such as mobile phone chargers, hair
straighteners etc.

All work on live fuse boards, lighting, sockets, power supplies and isolating
switches will be carried out by a qualified electrician and not by other staff.

If a fault is observed in the above mentioned supplies it should be reported
immediately to the Buildings Liaison Officer or other responsible person. A sign “Do
not use” should be placed on the supply.

Electrical plugs must be correctly fused for the equipment in use. No person may
connect a 13 amp plug to a lead until they have shown that they can do it correctly.
NB: On BCH site → only a qualified electrician may fit a plug.

All plugs and cables to electrical equipment must be checked visually for faults
before being switched on.

Extension leads should be uncoiled fully before use to avoid overheating. Use of
adapters should only be temporary and not part of a permanent supply. Adapters
should not be overloaded.

All electrical equipment if not in use should be switched off at the socket.

If a fault is observed in the supplies to a piece of equipment, the supply should be
switched off and the fault should be reported immediately to the Chief Technician or
other responsible person. A sign “Do not use” should be placed on the
supply/equipment.

Power supplies should be disconnected before repairing or dismantling equipment.

Do not attempt repairs yourself.
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6.12 Moving and Handling
6.12.1 Lifting and Carrying/Manual Handling:
No one should attempt to handle on their own a load which is excessively heavy or
bulky. Before any lift, Stop and Think:



Do I need help?
Where is the load going?
Are there any aids to help me?
The mechanical equipment provided should be utilized unless the load is well within
one’s personal capacity.
Equipment
available
Use for
Site
Location
Trolley
Moving boxes, reams of paper,
equipment etc
RVH
LG floor and store
BCH
Basement of Tower
Gas trolley
Transporting gas cylinders
RVH
Lab C
BCH
Store Cupboard
Bottle carrier
Transporting Winchester bottles
RVH
All labs
BCH
Post PCR lab
The Lift: In manual lifting it is the leg muscles which should be brought into use and
never the back, which may be wrenched rather easily. There should be no body twist or
jerk in picking up or setting down a load, and it is important not to over-reach. Always
face the load squarely and lift so as to ensure an even distribution on the feet. How to
lift:
 Feet apart
 Leading leg forward
 Bend the knees
 Lift in stages
 Keep back straight
 Keep load close to body
 Lean forward a little for good grip
 Keep shoulders level
 Get a firm grip
 Put down first, then adjust
For more detailed information please refer to the Safety Service website on Manual
Handling.
http://www.qub.ac.uk/directorates/HumanResources/OccupationalHealthandSafety/Manu
alHandling/
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6.12.2 Glassware handling and aerosols
Accidents with glassware represent one of the most common and re-occurring sources
of personal injury. This is most certainly a problem that can be prevented by initial
supervised instruction, followed by great personal care.
 Examine all glassware before use.
 Reject anything that is cracked or damaged.
 Do not store damaged glassware in cupboards, ensure it is disposed of properly.
 Do not overfill bottles, it is generally accepted that bottles should not be filled to
the neck. Max liquid level is at the bottom of the neck.
 Leave all glassware clean and tidy after use.
 Glassware used for heating must be suitable to withstand the temperature
 Report all breakage of glass to the Floor Manager.
 For transporting glassware, ensure to use an appropriate box to contain any
breakage.
6.12.3 Handling sharps
A sharps (needle stick) injury is an incident which causes a needle or sharp instrument
to penetrate the skin (percutaneous injury). If the sharp was contaminated with blood or
other body fluid, this has the potential to cause transmission of infection, including blood
borne viruses, for example, Hepatitis B, C or human immunodeficiency Virus (HIV).
Many percutaneous injuries are preventable. Training is essential before using sharps.
Please adhere to the following CPH safety guidelines to help reduce the risk:
 IMPORTANT! → Place all disposable sharps into a sharp container immediately
after use.
 Position the sharps container in a convenient, easily accessible location.
 Ensure that there are an adequate number of sharps containers available and
never overfill.
 Dispose of sharps container as clinical waste after closing securely, and replace
promptly.
 IMPORTANT! → Avoid resheathing needles manually. Only resheath needles if a
device is available to allow this to be done using one hand only.
 Discard disposable syringes and needles wherever possible as a single unit, into
sharps containers. Remove needles from syringes only when essential e.g. when
transferring blood to a container.
All accidents and injuries must be reported immediately to the Floor Manager.
More information is available at:
http://www.qub.ac.uk/directorates/HumanResources/OccupationalHealthandSafety/Guid
anceNotes/FullListofGuidanceNotes/
Under the section ‘Guide to Preventing Injuries from Glass and Sharps’
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6.13 Disinfection
It is of paramount importance that all items requiring disinfection or decontamination are
treated appropriately. This includes routine cleaning of lab benches and equipment,
servicing / repair of equipment as well as biological material or lab equipment for
disposal.
Products approved for use in CPH are:
Disinfectant/ decontaminant
Used for:
2% → Cleaning work tops
Trigene
10% → Blood and body fluids
Microsol 3+
Cleaning work tops and equipment e.g. pipettes
70% Ethanol
Cleaning surfaces in Tissue Culture
The correct protocol for disinfection / decontamination must always be followed. These
can be found on the S-Drive in the laboratory procedures folder.
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Section 7: First Aid
7.1 Basic advice about First Aid at work:
Trained First Aiders are located in each building (see section 1, emergency numbers).
First Aid kits are positioned in strategic locations where they are easily located (see first
aid provision, section 3.3.4). Safety showers are available for use in the labs.
What to do in an emergency (http://www.hse.gov.uk/firstaid/)
Priorities: Your priorities are to:
 assess the situation – do not put yourself in danger
 make the area safe
 assess all casualties and attend first to any unconscious casualties
 send for help do not delay
Check for a response: Gently shake the casualty’s shoulders and ask loudly, ‘Are you
all right?’ If there is no response, your priorities are to:
 shout for help
 open the airway
 check for normal breathing
 take appropriate action
 A → Airway: To open the airway: place your hand on the casualty’s forehead
and gently tilt the head back, lift the chin with two fingertips
 B → Breathing →Look, listen and feel for normal breathing for no more
than 10 seconds: look for chest movement, listen at the casualty’s mouth for
breath sounds, feel for air on your cheek. If the casualty is breathing normally:
place in the recovery position, get help, check for continued breathing. If the
casualty is not breathing normally: get help, start chest compressions (see
CPR).
 C → CPR →To start chest compressions: lean over the casualty and with your
arms straight, press down on the centre of the breastbone 5–6 cm, then release
the pressure; repeat at a rate of about 100–120 times a minute, after 30
compressions open the airway again, pinch the casualty’s nose closed and allow
the mouth to open, take a normal breath and place your mouth around the
casualty’s mouth, making a good seal blow steadily into the mouth while
watching for the chest rising, remove your mouth from the casualty and watch for
the chest falling, give a second breath and then start 30 compressions again
without delay, continue with chest compressions and rescue breaths in a ratio of
30:2 until qualified help takes over or the casualty starts breathing normally.
Severe bleeding: If there is severe bleeding:
 apply direct pressure to the wound
 raise and support the injured part (unless broken)
 apply a dressing and bandage firmly in place.
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Broken bones/ spinal injury: If a broken bone or spinal injury is suspected, obtain
expert help. Do not move casualties unless they are in immediate danger.
Eye Injury: All eye injuries are potentially serious. If there is something in the eye, wash
out the eye with clean water or sterile fluid from a sealed container, to remove loose
material. Do not attempt to remove anything that is embedded in the eye. If
chemicals are involved, flush the eye with water or sterile fluid for at least 10 minutes,
while gently holding the eyelids open. Ask the casualty to hold a pad over the injured eye
and send them to hospital.
7.2 Chemical contamination
First aid information is available on the MSDS for each individual chemical and also on
the COSHH form. For most chemicals, it is appropriate to flush contaminated skin or
eyes with plenty of water for 10 – 15 minutes whilst removing contaminated clothing and
shoes. Seek medical attention immediately, if necessary. Phenol is an exception:
EMERGENCY PROCEDURES FOR PHENOL BURNS:
 N.B. PHENOL DECONTAMINATING FLUID MUST BE AVAILABLE WHERE
PHENOL IS USED i.e. POLYETHYLENE GLYCOL (PEG) 300
If phenol has been SWALLOWED: IF POISONING OCCURS, CONTACT POISONS
INFORMATION CENTRE: ROYAL GROUP OF HOSPITALS 9.00 - 5.00 p.m. at 028
95040558 and out of hours 0844 8920111
IF SWALLOWED, DO NOT INDUCE VOMITING ..... give a glass of water
If phenol COMES INTO CONTACT with the EYES:
 Immediately hold the eyelids open if possible and flush continuously for 20 mins
with fresh running water
 Ensure complete irrigation of the eye by keeping eyelids apart and away from the
eye
 Transport to hospital (RVH Eye Casualty) without delay
 Removal of contact lenses after an eye injury should only be undertaken by
skilled personnel
If phenol COMES INTO CONTACT with the SKIN: Contamination of the skin with
Phenol and some of its derivatives may produce rapid collapse. BEWARE ..... standard
first aid treatment for chemical burns (washing the skin with cold water for 10-15 mins)
may increase systemic absorption and toxicity in the case of Phenol burns ..... thus:
IF SPLASHED on skin:




Wearing gloves, remove contaminated clothing and swab area repeatedly with
Polyethylene Glycol 300 (PEG) for 30 mins.
Cover lightly with sterile dressing
Treat for shock if required
Arrange urgent removal to hospital by ambulance if necessary.
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7.3 Burns and scalds: Burns can be serious so if in doubt, seek medical help.
Cool the affected part of the body with cold water until pain is relieved. Thorough cooling
may take 10 minutes or more, but this must not delay taking the casualty to hospital.
Certain chemicals may seriously irritate or damage the skin. Avoid contaminating
yourself with the chemical. Treat chemical burns in the same way as for other burns but
flood the affected area with water for 20 minutes. Continue treatment even on the way to
hospital, if necessary. Remove any contaminated clothing which is not stuck to the skin.
7.4 Electrical safety
If you suspect that someone has received an electric shock you must ensure all power
sources are isolated before you can treat the casualty.







Attempt to get the power turned off at the mains
Insulate yourself from the ground with books / newspapers / rubber mat.
Push away the power source using a wooden broom handle
Without delay, assess levels of response (see section 7.1)
Do not move the casualty unless the environment or situation is dangerous.
Shout for help and call emergency services.
Report the incident and remove faulty/ damaged equipment
7.5 Compressed gases including cryogenic liquids
Only enter the area if it is safe to do so. Oxygen levels may be depleted to a dangerous
level. Use an oxygen sensor to detect whether it is safe to enter.




Inhalation: Remove victim to fresh air. Keep warm and rested. Check for a
response (see section 7.1) and start CPR if necessary. Shout for help and call
emergency services without delay.
Eye contact: Flush the eyes with tepid water for at least 15 minutes. DO NOT
USE HOT WATER. Obtain medical assistance.
Skin contact: Flush or soak with tepid water for at least 15 minutes. DO NOT
USE HOT WATER. Apply a sterile dressing. Obtain medical assistance.
Ingestion: Seek medical attention immediately.
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7.6 Immediate action to be taken in event of sharps injury, splash or human body
fluid to eyes/mouth or human scratch/bite where exposure to possible infection
including blood borne viruses has occurred (also applicable to lone workers):

IMMEDIATE FIRST AID:
-
-
Puncture wounds should be encouraged to bleed freely by gentle
squeezing, but the wound should not be sucked. Wash wound with soap
and water, dry and apply dressing.
For splash to eyes or mouth use copious amounts of water to wash the
area thoroughly.

Report incident to your line manager or supervisor.

In conjunction with your manager assess the Blood Borne Virus (BBV) infection
risk – this will depend on the nature of the hazard associated with the incident.

Record on accident report form
Nature of hazard
Unused clean sharp which is
definitely uncontaminated
SOURCE BBV STATUS UNKNOWN
Used or dirty sharp
Human bite or scratch
Human body fluid splash to eye or mucous
membranes
Contamination of a cut or open skin lesion
Action to be taken
Complete accident report form.
If concerned seek further advice as below.
As soon as possible contact:
Mon-Fri 0900 – 1700
QUB Occupational Health Tel 9097 5520
SOURCE KNOWN TO BE HIV/HEP B OR
HEP C POSITIVE
Immediately contact for advice:
Used or dirty sharp
Human bite or scratch
Human body fluid splash to eye or mucous
membranes
Contamination of a cut or open skin lesion
Outside of normal hours contact:
Accident & Emergency Department at
RVH 90632250
Mater Hospital 90741211
Ulster Hospital 90484511
Mon-Fri 0900 – 1700
QUB Occupational Health Tel 9097 5520
Outside of normal hours contact:
Accident & Emergency Department at
RVH 90240503
Mater Hospital 90741211
Ulster Hospital 90484511
7.7 Requirement for an ambulance
Following a serious accident or illness call 9-999 for an ambulance.
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7.8 Accident reporting: All accidents, incidents and near-misses must be reported (see
section 2.7.11).
An accident, incident or near miss occurs
Without delay report to the Safety Officer:
Christine Belton (RVH)
Jill Kilner (BCH)
Roisin Martin (CRF)
The accident book and AC1 form must be completed
and/or appropriate Risk Management form
Accident is reported to Safety Services and RIDDOR if
necessary
CPH Safety Co-ordinators are informed imediately:
Jane McEneny, Niamh McIlherron, Cyril McMaster
Jane McEneny will ensure that School Office are
informed (Paula McDaid)
The Administrative Manager Niamh McIlherron will
record the incident
Incident is reported to appropriate local and school
safety comittee meetings
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Section 8: Fire Prevention and Control
8.1 The fire alarm
On hearing the fire alarm (usually a continuous loud siren) each person must evacuate
the building immediately.
8.2 Action
in the event of a fire
On hearing the fire alarm → evacuate immediately. Leave what you are doing and
vacate the building by the nearest fire exit. NB: Do not use the lift! Close fire doors
behind you to contain the fire. Do NOT stop to collect belongings or lock doors. Gather at
the fire Assembly Point:

RVH: Our assembly point is outside the MICROBIOLOGY BUILDING. Please
wait there until you are told to return to the building by the Fire Evacuation
Controller.

BCH: Fire exits are zoned for evacuation as explained at induction. Once in a
safe zone, make your way quickly to the ‘A’ Floor passenger lift lobby
assembly point and wait there until further instruction. Ensure the fire is
reported to extn 6666.
On discovering a fire or smelling smoke the break-glass fire alarm (red) must be
activated immediately (by breaking the glass). The fire alarm will sound automatically.
Evacuate immediately as described above. The fire brigade will be contacted
automatically. RVH Site: do not stop to use the phone. BCH Site: ensure the fire is
reported to extn 6666.
Once you have been successful in raising the alarm, you can consider using the fire
extinguishers. Do NOT do this unless:
 You are confident in the correct choice and use of the fire extinguishers provided.
 You have a colleague with you to ensure your escape route is clear.
 The flames from the fire do not appear dangerously high.
8.3 Training in fire safety
All staff and students must attend the annual CPH fire lecture. This is normally arranged
on RVH site and a signed register is kept.
All staff and students working on ‘A’ Floor, BCH site must also attend the annual
Regional Genetics fire lecture arranged by Padraig Hart.
8.4 Fire fighting equipment
It is the responsibility of each member of staff and student to know the location of fire
equipment (e.g. extinguishers and blankets), along with the location of the break-glass
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fire alarms (red), the fire escape routes and the emergency door release (green). Please
try to be aware of your nearest fire exit at all times.
Fire extinguisher: all fire extinguishers are coloured red. On each extinguisher, a
coloured band or label indicates the contents. In the area where you work, know the
location of the fire extinguishers and look closely to identify the type:
Each type of extinguisher has a specific use:
Colour of
band/
label
Contents:
White
Water
Blue
Dry Powder
Cream
Foam
Black
Green
CO2
Vapourising
liquids
Yellow
Wet chemical
Use on fire type:
Paper, fabric, wood, textiles
DO NOT use
on fire type:
Flammable liquids, live
electrical
Paper, textiles, flaming liquids
(oil, alcohol, solvents, gases)
and electrical
Flammable liquids
Electrical fires (switch off
supply first), burning liquids
(grease, fat, oil, paint)
Flammable liquids and live
electrical equipment
Paper, fabric, wood, cooking
oil
Electrical fires, cooking
oil
Not chip pan
Live electrical
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Fire Hose
Fire blanket
Fire doors
Keep the fire doors shut!
8.5 Fire Officers :
University Fire Safety Officer: Ciaran Connolly
Telephone: 028 9097 5311
E-Mail: Ciaran.Connolly@qub.ac.uk
Assistant Fire Safety Officer: Vacant
Telephone Extension: 028 9097 1112
E-Mail:
BHSCT Fire Officer (BCH): Vacant
Telephone: 028 9504 8820
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8.6 Fire Controller and fire Wardens
Fire Evacuation Controllers:
CPH building
Person responsible
Institute of Pathology
Cyril McMaster
Institute of Clinical Science, Block B
Niamh McElherron
Cancer Registry, Mulhouse
Colin Fox
CPH Fire Wardens:
Building
Area
Georgie Holmes and Claire Jess
nd
Sheree Hanna and Kathy McCrory
rd
3 Floor
Gerard Savage
Lower Ground Floor
Kathy Pogue and Dr Ann McGinty
Ground Floor
Christine Belton and Cyril McMaster
LGF/GF
Dr Sarah Gilchrist
1 Floor
2 Floor
ICSB
Responsible Person (s)
st
st
1 Floor
Gerry Clarke and John Murray
IOP
2nd Floor
Collette Devlin and Christine McMillan
Mulhouse
Cancer Registry
Colin Fox and Samantha Jameson
U Floor, CRF
Roisin Martin and Julie Wilson
Jill Kilner and Dr AJ McKnight
In addition to NHS operational managers:
Padraig Hart, Judith Briggs, and BJ.
BCH
A Floor, Nephrology
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Section 9: Spillages
The primary consideration for laboratory personnel when a chemical is spilled is safety.
Safety for every person in the laboratory and in the building is of paramount importance.
If the spill could potentially harm someone, tell the Floor Manager or Chief Technician
immediately. Otherwise, the laboratory worker who will clean up the spill must follow
specific procedures to do so safely and effectively.
9.1 Purpose, scope and responsibility

Purpose: To ensure that all spillages occurring within the Centre for Public
Health are dealt with in a safe manner, to ensure that the risk of harm to each
person and to the environment is minimised.

Scope: This procedure applies to all laboratory users

Responsibility: The Floor Manager is responsible for ensuring that adequate
spill response kits and suitable PPE are available at key locations. Each person
is responsible for familiarising themselves with the appropriate COSHH Risk
Assessments and MSDS.
9.2 Action in the event of an oil / chemical spill
Spill kits are located in each laboratory to ensure that they can be promptly availed of in
the event of a spillage. Information on handling spillages of specific hazardous
chemicals can be found in the CPH COSHH Risk Assessments located in each lab and
on the S Drive.
IMPORTANT! → In the event of a major spillage of chemicals, always contact your
Floor Manager or the Chief Technician immediately. It may be appropriate to call the
emergency services, QUB security (Tel 9097 2222) or QUB Environmental Manager (Tel
9097 1198).
In the event of spillage:
9.2.1 Small spillage:
Most small spills (<100 mL) do not require use of a spill kit. Small amounts of most
chemical can be either diluted with water, wiped up with tissue and disposed via the
laboratory waste disposal system, or else disposed down the sink with copious amounts
of water – see the appropriate COSHH Risk Assessment. If spillage is cytotoxic, e.g.
ethidium bromide, seek help from a senior member of staff who will deal with this spill!
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9.2.2 Large spillage:
1.
2.
3.
4.
5.
6.
Contact your Floor Manager or the Chief Technician immediately. Use the
chemical spill kit located in the laboratories – special kits are available for acid,
caustic, and solvent spills.
Keep people away from the spillage (at least 5 meters) and evacuate the area if
necessary in order to avoid contact with drift of fumes / dust.
Get help if necessary and warn others of danger. Do not leave the spill site
unattended until the danger has been removed.
If safe to do so, identify the spilled chemical, and read labels and specific
information contained in the MSDS and COSHH.
If spillage is flammable liquid, shut down all sources of ignition.
Wear the correct PPE. Goggles, chemical resistant gloves, boiler suit (RVH) and
face mask or respirator (RVH) are available if required.
9.2.2.1 If spillage is liquid:
7.
8.
9.
10.
11.
12.
13.
Do everything possible to keep the oil / chemical spill from spreading or getting
worse.
Control / stop the flow of liquid (if it is safe to do so) and contain the spillage in
as small an area as possible using minibooms and other sorbents.
Create a ‘dam’ around the spill preventing spilled material from entering
drains.
Working from the outer perimeter of the spill, place pads, granules, pillows or
cushions (for very large spill) provided on top of spillage to absorb liquid.
Replace saturated pads as necessary, until all the spill has been soaked up.
Wash spill site with water by keeping the ‘dam’ around the area and pouring small
quantities of water onto the spill area. Scrub gently with a brush and soak up using
absorbent pads.
Disposal of the waste: All used spill kit material is classified as a hazardous
waste and must be disposed of under Hazardous Waste Consignment Notes:
 Place all used spill kit and PPE into polybags provided in the spill kit.
 Close bags using cable ties (provided in the kit)
 Label clearly all bags used with ‘Contaminated Spill Kit’ and name of
the spilled substance.
 Store in designated waste storage area until safe disposal is arranged.
9.2.2.2 If spillage is powder:
14. Avoid raising a dust.
15. Carefully sweep up into a suitable, clearly labelled container using a dust pan and
brush provided in the spill kit.
16. Seal the container securely.
17. Wash the spill site thoroughly with plenty of water and ensure area is adequately
ventilated.
18. Dispose container and all contaminated tissue paper, PPE etc. as hazardous
waste (section 9.2.13).
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9.2.3 First aid: If skin/eye contact, inhalation of fumes or any other health concerns
have occurred, consult the First Aider and get medical attention if necessary. If
required, use First Aid Kit and safety shower. All contaminated clothing should be
removed.
9.2.4 Keeping a record: The completed Hazardous Waste Consignment Note(s)
must be retained for a period of at least three years to satisfy the legal requirements
under the Hazardous Waste Regulations. All incidents must be reported to the Safety
Officer.
9.2.5 Summary: Further information is available on the S-Drive under the Section
CPH Spill Response
9.3 Action in the event of a biological spill
In all cases, spillage of biological material must be reported to the Floor Manager or
Chief Technician. Always wear appropriate PPE – at least a buttoned up lab coat and
disposable gloves. Goggles and face mask or visor are recommended to protect against
splashing. Dispose of waste correctly via clinical waste container.
SITE
Spill
RVH
Human blood,
tissue and other
secretions
Whole Blood
BCH
Treatment
Large spills should be referred to a senior
member of technical staff for advice on
decontamination. Hazard signs should be posted
on laboratory doors if a significant area has been
affected until decontamination is complete.
If spillage is contained (e.g. on a spill tray)
solidify with Verna gel and transfer all
contaminated items to yellow rigid container with
yellow lid for disposal.
If spillage is not contained (e.g. on the floor)
add Trigene 10% solution. Lift into suitable
container and dispose in yellow rigid container
with yellow lid. Wash the spill site thoroughly with
Trigene 2% solution.
Dispose of all contaminated tissue, labcoats and
gloves etc. in the yellow rigid container with
yellow lid.
DNA
Wash area with Microsol 3+ decontaminant and
copious amounts of water. Place all
contaminated tissue, plastics etc. in yellow rigid
container for incineration. Use the eye wash in
the laboratory or safety shower if necessary.
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9.4 Action in the event of a mercury spill
Spillage of mercury may occur when a high pressure mercury vapour lamp (e.g. in a
fluorescence microscope) explodes, or if a mercury thermometer is broken.
NB: mercury thermometers are no longer in routine use in CPH and their use should be
reported to the Chief Technician.
Procedure:
1. If a mercury bulb explodes, evacuate the room immediately.
2. Post a warning sign and prevent others from entering.
3. Without delay, report the spillage to the Floor Manager or Chief Technician.
4. Ensure that no one enters the room for at least one hour to allow the mercury
contamination in the air to return to a safe level.
5. When the lamp has cooled, use a mercury spillage and decontamination kit to
pick up any mercury residue that has settled on surrounding surfaces, or to pick
up any liquid mercury spills from a broken thermometer.
6. Dispose of the mercury residue as instructed in the spill kit.
7. DO NOT dispose of broken thermometers in the Magpie box. These must be
treated as mercury waste.
9.5 Action in the event of a Radioactive spillage
IMPORTANT:


For all radioactive spills contact the RPS without delay.
DO NOT attempt to clean up this spill yourself.
Refer to the specific information advised in ‘Appendix A’ and ‘Local Rules Doc 4’.
Further information is available from USS at:
http://www.qub.ac.uk/directorates/HumanResources/OccupationalHealthandSafety
/GuidanceNotes/IonisingRadiation/
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9.6 Action in the event of a Cryogenic gas (e.g liquid nitrogen), or dry ice
spillage
Spillage of liquid nitrogen or dry ice must be treated with great caution. The hazards
associated with this spill include:
 Cold burns – because of the low temperature of liquefied / solidified atmospheric
gases (-196°C) the solid, liquid, cold vapour or gas can produce skin damage
similar to heat burns. Unprotected skin may stick fast to cold equipment and the
flesh may be torn on removal.
 Frostbite – may be caused by cold vapours or gas after prolonged or severe
exposure of unprotected skin. Local pain may give a warning, but often little or
no pain is felt. Frozen tissue is painless with a waxy, pale yellowish colour.
Thawing can cause intense pain and shock.
 Effect of cold on the lungs – Brief exposure to very cold gas causes discomfort
to breathing and can trigger an asthma attack in susceptible persons.
 Hypothermia – resulting from very low air temperature. Symptoms include
slowing down of mental and physical responses, difficulty with speech or vision,
unreasonable behaviour, irritability, cramp and shivers.
 Asphyxiation – nitrogen, argon, helium and carbon dioxide are simple
asphyxiants when present in high concentrations in air, particularly in confined
spaces.
NB: Do not attempt to clean up this type of spill with any spill kit!!
Never dispose of liquid nitrogen or dry ice down the sinks or drains
Procedure:
1. Evacuate the area immediately.
2. Post a warning sign to prevent others from entering.
3. Without delay, report the spillage to the Floor Manager or Chief Technician.
4. Allow the spill to evaporate (caution! → this may take considerable time
depending on the volume of the spill). If safe to do so, ensure that the area has
plenty of ventilation.
5. Administer first aid if necessary (Section 7.6)
6. After all spilled material has evaporated and the area has been thoroughly
ventilated, check the area for damage. Cryogenic liquids can cause materials to
become brittle and may cause cracks in flooring.
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Type of spill
Small spill
Extra PPE
Spill
granules
required
Add
water

Add
Microsol
Spill kit
required
Other notes

DNA
Blood
Use full face
visor
and
nitrile gloves
Clean and
contaminate
area
with
Trigene
Acid/alkali spill
Use
heavy 
disposable
gloves
If large Get help
spill
Solvent spill

Use
respirator
and
heavy
disposable
gloves
If large Get help
spill
Powder/ pellet Use
eye
spill
protection,
face
mask
and
nitrile
gloves
Chemical/
alcohol spill
Use
face 
mask
and
nitrile gloves
Place
in
suitable
container
and
label
clearly.
Never mix
chemicals
for disposal.

If large Isolate
spill
area
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the
Section 10: Control of substances hazardous to health (COSHH)
Working in the laboratory requires you to be familiar with the location and
information contained in the COSHH Risk Assessments.
10.1 What is COSHH
COSHH is the law that requires employers to prevent or control and reduce workers
exposure to substances that are hazardous to health.
(The Control of Substances Hazardous to Health Regulations (COSHH) originally came into force in
October 1989)
Sometimes substances are easily recognised as harmful. Common substances
such as paint, bleach or dust from natural materials may also be harmful.
The Centre for Public Health controls the use of substances hazardous to health in the
following manner:
 All hazardous substances are assigned a unique CPH ‘COSHH number’
indicating that an information sheet has been prepared recording the hazard
categories, WEL limits, PPE recommendations, spillage action, and disposal
requirements.
 All laboratory ordering is monitored by the Chief Technician to ensure COSHH
compliance.
10.2 Why COSHH matters
Using chemicals or other hazardous substances at work can put people’s health at risk,
so the law requires employers to control exposures to hazardous substances to prevent
ill health.
Employers have to protect their employees and others who may be exposed, by
complying with the Control of Substances Hazardous to Health Regulations 2005
(COSHH) and amendments.
Latest amendments can be downloaded from the Health and Safety Executive website;
www.hsebooks.co.uk
The effects from hazardous substances range from mild eye irritation to chronic lung
disease or, on occasions, death.
Each year, approximately 16,000 to 25,000 people become ill as a result of
exposure to substances hazardous to health at work, e.g. respiratory disease,
dermatitis etc. Included in this range is an estimated 3,000 to 12,000 cancer deaths
mostly related to chemicals (including asbestos).
Failure to comply with COSHH regulations can lead to prosecution and civil claims.
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COSHH covers substances which cause ill-health, including:





Substances labelled corrosive, irritant, harmful, toxic or very toxic
Respiratory sensitizer (dust, fumes etc)
Micro-organisms (viruses, bacteria)
Carcinogens, mutagens or teratogens (agents toxic to reproduction)
Substances that are flammable or explosive
10.3 Completing a COSHH Risk Assessment for laboratory procedures (PRA)
This is a number controlled document according to laboratory, listing all of the chemicals
used in each laboratory procedure and provides a combined risk rating. These are found
on the S drive and grouped into laboratory sub-folders.
These must be read and signed and are retained by the Chief Technician or the
Floor Manager in Nephrology/BCH.
As well as listing all the chemical hazards, the PRA looks at the experiment as a whole
considering, for example, how the chemicals are used during an experiment e.g.
quantities, properties (liquid, powder or gas etc), length of exposure, whether they are
heated, poured, sprayed etc. COSHH Essentials is a useful tool for estimating airborne
concentrations of substances and provides advice about control measures required and
PPE http://www.hse.gov.uk/coshh/essentials/
Instruction on the completion of a COSHH risk assessment is given in Appendix B. For
queries and advice about COSHH, please contact the CPH COSHH supervisor,
Christine Belton.
10.4 COSHH Chemicals A-Z
COSHH chemical forms are information sheets on the safe use of all chemicals. The
Centre for Public Health maintains this information in a COSHH A-Z database which
provides information (taken from the MSDS and product information sheet) for each
chemical used in the lab. All hazardous chemicals are assigned a COSHH chemical
index number and this must be quoted on purchase order requisitions each time the
chemical is ordered. COSHH chemical A-Z is found in labs and on-line on the S drive,
which is more up to date.
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Section 11: Risk Assessment
Working in the laboratory requires you to be familiar with the location and
information contained in the Risk Assessments.
11.1 General
Before commencing any work in the laboratory you must read and sign all of the
relevant risk assessments and complete a new one if necessary in collaboration with
your supervisor. A Risk Assessment must be completed for every procedure/experiment
conducted in the laboratory. The Chief Technician retains all of the Risk Assessment
forms.
Risk Assessments will be carried out in association with COSHH (Control of Substances
Hazardous to Health) documentation (Appendix B).
11.2 Completing a risk assessment
A Risk Assessment identifies the hazards associated with an experiment/procedure. The
hazard is the potential to cause harm. The risk is the likelihood that harm will arise in the
actual circumstances of its use. By its very name a Risk Assessment assesses how
much harm will be done if the identified hazard causes an accident/incident. The risk will
depend upon:
• The hazard presented by the substance or equipment
• How it is used
• How exposure is controlled
• To what extent people are exposed and for how long
• Whether individuals are particularly vulnerable to it
• The work which is being done
For example for substances there is substantial risk, even from a substance that is not
particularly hazardous, if exposures are excessive, but with proper precautions the risk
of being harmed by even the most hazardous substance can be very small.
When using hazardous chemicals the first priority is prevention of exposure and the
second is control.
Prevention might be achieved by:
• changing the process of exposure, activity or method of work so that hazardous
substances are not required or generated.
• substitution by a safer alternative, or
• using the substance in a safer form
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When prevention is not reasonably practicable, exposure must be adequately controlled
by measures other than personal protective equipment. Control measures might be
achieved by:
• total enclosure of the process
• partial enclosure with extraction ventilation
• local exhaust ventilation
• sufficient general ventilation
• using systems of work and handling procedures which minimise the chance of spills,
leaks, and other escapes of hazardous materials.
If, and only if, exposure cannot be controlled by any combination of the above – then
personal protective equipment may be used.
A thorough and proper Risk Assessment completed at the same time as COSHH
documentation (Section 10), will incorporate all of the above and lead to safe working
practices.
11.3 Equipment Risk Assessment (ERA)
This is a number controlled document by laboratory and lists possible operational
hazardous and checks to be made before switching on. These are found on the S drive
and grouped into laboratory sub-folders. The following is an example of an equipment
risk assessment:
Centre for Public Health
Equipment Risk Assessment Form: ERA- 006
Name of assessor :
Status of assessor :
Cyril McMaster
Chief technician
Activity or equipment being assessed :
Centrifuges/Microfuges
Who might be harmed: Staff; students; visitors.
Known or expected hazards associated with the activity :
Mechanical failure of moving parts (often violent), contact with moving
parts, leaks causing corrosion, contamination and ill-health, imbalance
causing machine
movement or stress failure, fire, explosion.
Possible effects of hazards : Injury, infection.
Severity of possible effects (1 = minor, 2 = serious, 3 = major)
Likelihood of effects ( 1 = unlikely, 2 = likely, 3 = very likely)
Risk factor
: 3
: 2
: 6
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Measures to be taken to reduce the level of risk :
•
•
•
•
•
•
•
•
•
•
•
•
•
•
No one is allowed to use a centrifuge until they have convinced their supervisor that
they are fully conversant with its operation.
Before use, check that all parts of the spindle, head and buckets are clean,
corrosion and crack free, and secure.
Loads must be distributed symmetrically in accordance with manufacturers
instructions.
Buckets must be matched and seated correctly in the rotor head. Loads must
always be balanced for weight (except for green RIA racks which may be matched
visually). Tubes must not be overfilled.
Solvents or cleaning fluids should be checked for compatibility with the centrifuge
materials before being used.
Combustible solvents should not be used in centrifuges, as their vapours
may be sucked into the air system and ignited by the sparks from the
electric motor.
•Biological fluids should be centrifuged in air tight containers. These
should be sterilized non-corrosively immediately after use as appropriate.
All centrifuge heads must be firmly attached to spindles, locking keys in position,
and nuts tightened.
Rubber cushions must be used in those buckets requiring them.
The lid of refrigerated centrifuges should be closed all the time that they are
switched on, even though the rotor may not be rotating. This reduces the build up of
condensation and prevents the temperature rising above 4oC.
The rotor must be brought up to speed slowly, and maximum speed for the head
must not be exceeded.
The user must remain with the centrifuge until it has stabilised at the required speed.
•The lid of the centrifuge must not be opened while the rotor is moving,
even slowly, and the rotor should be allowed to slow down automatically, not
by switching off the mains supply.
• Any liquid spilled in the centrifuge should be cleaned up immediately.
Spilled liquids can cause corrosion, one of the main causes of centrifuge
failure, or form dangerous aerosols that will be dissipated throughout the
surrounding area.
Any signs of corrosion must be reported to a responsible person immediately.
Training prerequisite : Training by experienced staff is essential
Procedures in event of emergency or system failure : CLEAR UP any spills immediately,
especially if biological fluids are being processed, in accordance with local safety
measures.
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Any faults must be reported immediately, the centrifuge put out of commission,
and the defect repaired by a competent person.
11.4 Task Risk Assessment (TRA)
This is also a number controlled document combining the PRA and all of the equipment
ERAs required and takes into consideration, the locations, any movements between
labs, others working around you etc and an overall risk rating is applied.
These must be read and signed and are retained by the Chief Technician the Floor
Manager in Nephrology/BCH.
RISK ASSESSMENT GUIDANCE NOTES AND BLANK FORMS CAN BE FOUND ON
THE S DRIVE
Section 12 Laboratory Procedures
12.1 Laboratory Procedure Document Control (LP)
This is a step by step guide on how to conduct a
procedure/analysis/experiment. Similar to following a kitchen recipe.
LP documents are found on the S drive in the LP Document control folder.
laboratory
12.2 Equipment Standard Operating Procedures (SOP)
This is a list of steps on how to use a particular piece of laboratory equipment.
Specialist laboratory equipment requires extra training before use. For example:
RVH site → ultracentrifuge, HPLC, LCMSMS, Triturus ELISA machine, ILAB chemical
analyser
BCH site → Hamilton Microlab STAR, Ion Proton sequencer.
Do not attempt to use any of these until you have been trained.
Equipment SOPs are found on the S drive.
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Section 13 Radiation safety procedures
13.1 Ionising radiation, supervised areas and staff responsible
A radiation laboratory (“hot room”) is located on the LGF of the Pathology Building. This
lab is restricted to trained personnel only.
Dr Sarah Gilchrist is the Centre Radiation Protection Supervisor and Christine Belton is
the appointed deputy.
All radiation work must be arranged with Sarah well in advance and all orders of
radioactive substances must be copied to Sarah and processed via the Ferguson
Building, Belfast Hospital Trust.
13.2 Ionising radiation safety policy
For specific information regarding working with radiation please see the QUB ionising
radiations safety policy on-line and Appendix A.
And Local Rules Doc 4
13.3 Artificial optical radiation (AOR)
Includes light emitted from all artificial sources e.g. ultra violet, infrared, and laser beams
(excludes sunlight). Most light sources are safe e.g. ceiling lights with diffuser fitted,
computer monitor, photocopier. Employers are required to protect the eyes and skin of
workers from exposure to hazardous sources of AOR. Hazardous sources of light
used in CPH that can damage eyes and skin on exposure are:
 UV transilluminator
 UV light box
 UV lamp in biological safety cabinet
 Blue light transilluminator (E-gel system)
 Class 2 and Class 2M lasers e.g. bar code reader
13.4 UV transilluminators
UV transilluminators are used to visualise DNA, RNA and proteins in agarose gel
electrophoresis. Exposure of the skin to UV radiation causes severe skin / eye damage
and burns. Users MUST protect themselves from exposure.
 Use the safety cabinet or shield provided.
 If excising bands from gels, wear a protective face visor (remember it only
protects the person wearing it!)
 Wear suitable gloves and arm protection to protect the skin on hands, wrists and
arms. If necessary, tape gloves to lab coat sleeves.
The E-gel blue light transilluminator (BCH site) does not use UV radiation. Light emitted
can, however, cause damage to the eyes and the amber safety spectacles and shield
must be used.
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Section 14 Compressed gases
14.1 Gas Safety and Liquid Nitrogen Handling
All users of gas must read the QUB Safety Services “Gas Safety Guidance”
document, Ref: SS/GU/010/1. Gas and Liquid Nitrogen cylinders should only be
transported by trained personnel. Liquid Nitrogen must NEVER be accompanied in a lift.
If you require gases to be transported please contact the Chief Technician.
14.2 Handling Liquid Nitrogen
Liquid Nitrogen is widely used as a source of refrigerant. It is typically stored in vacuum
insulated vessels (dewers, liquid cylinders etc.).
Liquid Nitrogen must be handled with great care because of two of its properties:
 It is cryogenic liquid at a temperature of about -196 deg C which can cause
severe cold burn
 When vaporised to gaseous nitrogen, it is an asphyxiant which is colourless,
odourless and tasteless
Personal protective equipment is vital when handling liquid nitrogen:
 Wear suitable gloves
 Wear suitable eye and face protection
 Wear appropriate footwear
 If working in a confined or poorly ventilated space, if possible use an oxygen
monitor.
Further information and Risk Assessments are available on the S drive;
Key Message: Don’t take risks. If in doubt, stop and seek advice.
14.3 PTFE Tape and Oxygen Service:
PTFE tape is used in a variety of applications for sealing gas and liquid pipework, and is
available in various grades, differing in purity, thickness and width. Some grades of tape
contain a large proportion of material other than PTFE, and may be lubricated with
hydrocarbon based oils and greases. If these grades of tape come in contact with high
pressure oxygen there is a chance that an ignition will occur with the risk of injury to the
operator and damage to equipment.
Pure PTFE tape can be, and is, used for sealing components in oxygen service provided
it is lubricant-free. Even then it should be applied with care by experienced personnel to
ensure that loose strands are not exposed to high velocity gas streams. Thus, oxygen
cylinders and regulators prepared by or for BOC do have PTFE tape on them but only for
certain applications and used only in accordance with strictly controlled procedures.
Because of the possibility of ignition if a low grade of PTFE tape is used or if tape is
incorrectly applied, BOC does not recommend its use by customers for oxygen service.
In particular, the practice of using PTFE tape to form a seal between the oxygen cylinder
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valve outlet and regulator can be especially high risk. If you experience difficulty in
producing an effective seal without the use of PTFE tape, the surfaces of the valve outlet
and regulator bull nose should be examined for damage and then refer any problems to
the supply branch or Gas & Gear centre.
14.4 Gas Equipment Safety Checks:
Annual onsite inspection by a fully qualified inspector. Thorough functional and leak
testing of regulators, flashback arrestors, hoses, torches and associated fittings. Full
report of findings. Certificate of compliance to be retained.
14.5 Gas welding and cutting equipment must be checked regularly:
The British Compressed Gases Association (BCGA’s) Code of Practice 7 (CP7) (the
safe use of oxy-fuel gas equipment) and Guidance Notes 7 (GN7) (the safe use of
individual portable or mobile cylinder gas supply equipment) recommends that regulators
and flashback arrestors be checked annually and replaced every five years.
Checks need to be undertaken by a person who has sufficient practical experience of
oxy-fuel gas equipment and theoretical knowledge of the functioning of the equipment,
the properties of gases used, the potential defects and hazards that may occur and their
importance to the integrity and safety of the equipment.
BOC CP7 checks are available and are performed by a qualified BOC Industrial
Inspector (certified by Btec). The inspection includes:
A thorough functionality and leak test of all components – regulators flashback arrestors,
hoses, torches and associated fittings;
A full report of the inspector’s findings and Certificate of Compliance can be found on the
S drive.
Please Note:
Gas equipment can be a hazard at work if it is not maintained properly. Using unsafe
equipment or failing to test equipment fully could result in accidents and serious injury in
the workplace.
Under Section Safety, Health and Welfare at Work Act 2005, an employer or selfemployed person has a responsibility to provide and maintain plant and systems of work
that are, so far as reasonably practical, safe and without risk to health.
Under Safety and Welfare at Work General Applications (Amendment) 2007: Require the
user of an installed system and the user of a Mobile system to ensure that the system is
properly maintained in good repair so as to prevent danger. Statutory Instrument 2000
No 128: The Pressure Systems Safety Regulations 2000 requires equipment to be
examined regularly.
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Section 15: LABORATORY WASTE DISPOSAL
The disposal of experimental and other waste is the responsibility of each member of
staff and student. It is essential to determine safe routes of disposal that do not pose a
threat to other research workers, cleaners or the environment and are in accordance
with the specific COSHH requirements.
The following is a summary of our routes of disposal, you should read the appropriate
guidelines specific to the materials being used as detailed on the relevant COSHH form.
15.1 Waste can be classified as:
1. Microbiological
2. Cell and Tissue culture
3. Human blood, tissues and other secretions
4. DNA and DNA containing products
5. Sharps
6. Solvents
7. Glassware
8. Chemical
9. Contaminated lab coat
10. Recycling
11. Confidential
12. Equipment
After reading the notes any queries should be directed to your Floor Manager or the
Chief Technician.
NB: IMPORTANT!
 PLEASE KEEP CLINICAL WASTE DISPOSAL TO A MINIMUM AS IT IS
COSTLY TO DISPOSE OF.
 DO NOT PUT UNNECESSARY ITEMS IN CLINICAL WASTE!!
 PLEASE ADHERE TO THE FOLLOWING GUIDELINES CAREFULLY.
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15.1.1 MICROBIOLOGICAL
(Applicable on RVH site only)
Liquid bacterial cultures: Must be sterilized before disposal. Generally this should be
achieved by steeping the container in 2% Trigene overnight then placing them into the
clear autoclave bags for autoclaving. In exceptional circumstances (e.g. radioactive
cultures), autoclaving may be inappropriate in which case consult your Supervisor.
Plastic tubes, pipettes and Petri dishes: Must be autoclaved. They should be placed
in the clear autoclave bag that is placed in an open sharps box so to retain any liquids
(especially molten agar) during autoclaving. After autoclaving the waste bag should be
removed and disposed of in the blue dumpster. Do not leave for domestic staff.
Contaminated syringes, needles and sharps: are to be placed in the orange top
sharps boxes or the purple top if cytotoxic.
15.1.2 CELL AND TISSUE CULTURE PROCEDURES
(Applicable on RVH site only)
Human Cells: Never attempt to culture your own cells or those of other individuals who
have access to our laboratories. All manipulations of human cells should be performed in
a class II laminar flow hood. Incubators holding human cells should carry a warning
label.
Disposal of infected cells: The infected cells, media and container must be transferred
to the clear autoclave bags. The technical staff will autoclave this bag and transfer in
preparation for disposal by domestic waste route in a blue bag. Do not leave such waste
for the cleaners to remove and ensure that your sample does not contain a large
quantity of liquid waste which may leak or burst on contact in the waste bins.
Disposal of plastic pipettes used for media transfer: These should be collected in the
waste pipette holder by the work station which contains 10% Trigene. When the holder
is full the technician will seal the container and autoclave it.
Disposal of infected and non-infected culture media: The media should be mixed
with 10% Trigene overnight before being washed down a designated sink with copious
amounts of cold tap water.
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15.1.3 HUMAN BLOOD, TISSUE AND OTHER SECRETIONS
15.1.3.1 Applicable on RVH site only : Handling procedures
 Disposable gloves and laboratory coats should be worn at all times.
 Eye protection should be worn if there is a risk of blood splashing.
 A disposable face mask should be worn for certain secretions where there is risk
of sample aerosol effect (e.g. nasal fluids and broncho-alveolar lavage fluids).
 Appropriate vaccinations (e.g. Hepatitis) should be considered before starting
work.
Decontamination of blood or secretion spillage: After routine procedures all surfaces
and sinks and dissecting instruments to be washed with 2% Trigene and 70% alcohol.
When a spill of blood or other secretion has taken place this should be decontaminated
with a solution of 10% Trigene. All surplus blood samples should be recapped and
placed in a yellow bin with a yellow lid.
Decontamination of Centrifuges: To avoid inhaling aerosols face masks should be
worn when dealing with breakages in centrifuges. Where they are present bucket lids
must be fitted. If a breakage occurs in a centrifuge where a bucket lid is fitted the whole
bucket may be removed from the centrifuge. Broken glass should be placed in the
broken glass box or the rigid burn bins provided for the disposal of blood tubes. Buckets
should be decontaminated by soaking in 2% Trigene or other suitable disinfectant for at
least 30 mins and thoroughly rinsed and dried before reuse. If bucket lids are not in
place the lid of the centrifuge should be kept closed for at least 30 mins before
decontamination is carried out to avoid inhaling any aerosols. All tissues and gloves
used must be disposed of in clinical waste bags provided.
Large spills should be referred to a senior member of technical staff for advice on
decontamination. Hazard signs should be posted on laboratory doors if a significant
area has been affected until decontamination is complete.
Waste disposal: Disposable containers with blood residue, gloves, absorbent bench
protector and all contaminated tissues should be placed in a large yellow container with
yellow lid.
Contaminated sharps: (scalpel blades, syringe needles etc) and used glass vials
should be placed directly into a sharps box container. Handling of these items should be
kept to a minimum to reduce the risk of accidental stab and needle stick injuries.
Tissue disposal: Unfixed human material. This should be placed in a clear autoclave
bag labelled with a tag identifying the user. After sterilization these should be sealed in
yellow biohazard bags labelled as containing human material.
Fixed human material. Material fixed in aldehyde fixatives should be sealed in a labelled
polythene bag and transferred to a yellow rigid container with yellow lid.
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15.1.3.2 Applicable on BCH Site only: Handling procedures for biological fluids:
Laboratory coats and disposable nitrile gloves should be worn at all times.
Full face visor should be worn to protect against splashes.
Appropriate vaccinations (e.g. Hepatitis) should be arranged before starting work.
 Decontamination of blood spillage: After routine procedures all surfaces and sinks
should be washed with 2% Trigene. When a spill of blood has taken place,
decontaminate by adding 10% Trigene, absorb onto tissue and place into rigid yellow
container with yellow lid. The area should be decontaminated with a solution of 2%
Trigene.
 Waste disposal: Verna Gel should be added to all surplus blood samples before
disposing in a rigid yellow container with yellow lid.
 Disposable containers with blood residue should be solidified with Verna Gel and
placed in the large rigid yellow container with yellow lid. Swabs should be placed in
the yellow rigid container with yellow lid.
 Contaminated sharps: (scalpel blades, syringe needles etc.) and small used glass
vials should be placed directly into a sharps box container with purple lid. Handling of
these items should be kept to a minimum to reduce the risk of accidental stab and
needle stick injuries.
 Disposal of plastic pipettes: These should be collected in a plastic beaker
containing 10% Trigene. Ensure that the Trigene solution is drawn into the pipette
bulb and allow to soak overnight before disposal in a yellow rigid container with
yellow lid.
15.1.4 DNA and DNA containing products:
Applicable on BCH site, A Floor only: Handling procedures
Laboratory coats and disposable nitrile gloves should be worn at all times.
Eye protection should be worn if there is a risk of splashing.
 Decontamination of DNA and DNA products: After routine procedures all surfaces
should be wiped down with MicroSol 3+ provided under the sink.
 Contaminated sharps: All sharps (including pipette tips) should be disposed directly
into the sharps box with purple lid for incineration.
 Waste disposal – non toxic waste: Tubes, plates and tissue etc that have been in
contact with DNA should be placed in the yellow rigid container for incineration.
 Waste disposal – *toxic waste*: Tubes, plates, gels and tissue etc. that have been
in contact with DNA and are also contaminated by a cytotoxic agent, mutagen or
carcinogen (e.g. picogreen reagent, Qubit reagent, SYBR Safe DNA gel stain)
should be placed in a yellow sharps box with purple lid.
 Sequencing plates: Remove septa and wash or dispose of in the yellow rigid
container for incineration. Place plates in the yellow rigid container for incineration,
once full, close and tag for disposal.
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15.1.5 Sharps
Needles and contaminated syringes: should be placed in the sharps box containers
provided.
Sharp plastic ware, chips, etc:
 RVH Site: Pipettes/pipette tips should be placed in a yellow clinical waste bin.
 BCH Site: All sharps including pipette tips, blunt needles, Sequenom chips, Proton
or PGM chips and Bioanalyser chips should be placed into the purple top sharps box
for incineration.
15.1.6 Chemicals
 All appropriate COSHH forms must be read and signed before handling any
chemical.
 Please ensure that the appropriate PPE is worn at all times.
 All chemical waste should be disposed of as detailed on each individual MSDS
sheet or as explained on the relevant COSHH form. Small amounts of many
chemicals (<100 mL) can be disposed down the sink or via the lab disposal routes.
 QUB Safety Services organise an annual collection of waste chemicals.
IMPORTANT! → Chemicals that require disposal via the University Annual
Chemical Disposal Lift (normally takes place in August) must be correctly stored
until collection. Please notify the Floor Manager who will complete the waste
chemical record form on the S-Drive and ensure correct storage until the next
collection time. The Chief Technician will submit the final record of all CPH waste
chemicals to Safety Services.
 Please note that Picogreen, SYBR Safe, Qubit reagent, e-gels, PeqGreen, ethidium
bromide and all plastics or tissue contaminated with the same must be placed in a
yellow sharps box with purple lid for disposal. Gels containing SYBR Safe,
PeqGreen or ethidium bromide must also be placed in the purple sharps box.
15.1.7 Solvent and alcohol disposal
 All appropriate COSHH forms should be read and signed before handling.
 Please ensure that the appropriate PPE is worn at all times.
 Small amounts of alcohols (<100 mL) can be disposed of down the sink followed by
copious amounts of water.
 Do not put solvents down the sink that are immiscible with water. Waste
solvents must be put into a clean Winchester bottle or suitable container and clearly
labelled with the contents along with whether the contents are chlorinated or nonchlorinated waste. The labelled waste container should be placed in the designated
locked waste solvent cupboard. Please inform your Floor Manager who will make
arrangements for appropriate disposal at an arranged date with USS.
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15.1.8 Broken glassware and aerosols
Broken and uncontaminated or decontaminated glass should be placed in the labelled
cardboard broken glass ‘Magpie’ box. They must not be put into any other bin. Aerosol
cans should also be disposed via this route.
15.1.9 Contaminated lab coat
Lab coats contaminated with any hazardous chemical or biochemical hazard should be
placed in the yellow rigid container with yellow lid (RVH) or yellow rigid container with
purple lid (BCH). If cytotoxic they must be placed in a sharps box with a purple lid,
closed and tagged for disposal.
15.1.10 Spillages
Refer to the Laboratory Spillages Protocol for advice about how to deal with
specific spills.
 Chemical Spillages: Must be dealt with immediately. Always dilute small
chemical spillages with plenty of water before mopping them up. Always wear
appropriate gloves and safety glasses and consult the relevant COSHH form for
safe methods of disposal.
 Acid/alkali spillages must be treated with extreme care. When water is added to
acids and alkalis, heat and fumes are evolved, therefore if the spillage is more
than just slight, it should be absorbed onto an inert medium e.g. sand or "spillage
absorption granules" obtained from the spill kit available in the lab. Dispose of
gloves, tissue paper etc. in yellow rigid container with yellow lid (RVH) or yellow
rigid container with purple lid (BCH).
 Never attempt to clear up a large chemical spillage single-handed. All spillages
must be reported to the Floor manager or Chief Technician.
 Biological spills – For DNA add MicroSol 3+ and leave for 2-3 minutes. Wearing
gloves, carefully mop up the fluid mixture with tissue paper and place in the
yellow rigid container for incineration. Wash spill site with MicroSol 3+.
 In the case of blood, add 10% Trigene and wearing gloves mop up with tissue
paper. Dispose of contaminated tissue paper in the yellow rigid container with
yellow lid. Decontaminate the spill area by washing with 2% Trigene solution.
 Powder spillages should be swept up and put into a suitable container. This
container should then be labelled clearly and given to the Floor Manager for
disposal. Never mix chemicals for disposal.
 Radioactive spill – the RPS will arrange disposal of this spill.
All major spills should be reported to the Floor Manager or Chief Technician
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15.1.11 Laboratory Equipment, Electrical and Electronic Equipment (WEEE)
 All obsolete/surplus furniture and WEEE must be disposed of in an
environmentally friendly manner.
 DO NOT PUT ELECTRICAL WASTE INTO SKIPS OR BINS!
 Equipment (excluding computers) that may be of use elsewhere in the University
may be sold (see Purchasing Office website).
Computers: If you wish to dispose of a computer please seek permission from your
supervisor/line manager (BCH site: please inform Dr A.J.McKnight). Next, contact
the CPH Inventory Officer, who will update the CPH equipment inventory register.
IMPORTANT: DO NOT remove the inventory sticker. Finally, email the BLO who will
arrange collection and disposal according to QUB policy.
Lab equipment: Disposal of laboratory equipment should be arranged via the Chief
Technician. It is the users responsibility to ensure that all items for disposal are
appropriately cleaned. If equipment has been used in the lab (or other potentially
hazardous setting), it must be decontaminated and certified safe before collection.
Contact the CPH Inventory Officer, who will update the CPH equipment inventory
register. IMPORTANT: DO NOT remove the inventory sticker. Certain items of
equipment e.g. MJ Tetrad PCR machines may be returned to the manufacturer for
disposal.
Other brown and white electrical equipment: All other obsolete/surplus furniture
or electrical equipment that is beyond economic repair, including computer monitors,
fridges, freezers, microwave ovens, etc. must be disposed by e-mailing the BLO who
will contact estates and arrange collection. Please include the equipment inventory
number and room number. IMPORTANT: DO NOT remove the inventory sticker. A
minimum number of major items must be accumulated across the University before a
free collection can be arranged. Otherwise there may be a charge for disposal of
these items via the waste contractor. Examples of prices are on QUB Estates
website.
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15.1.12 Waste for Recycling
 Ink/ toner cartridges: should not be disposed in general waste but should be
recycled via the CPH admin office, ISCB (RVH site) or in the envelope provided
or else returned to the manufacturer (BCH site).
 Cardboard boxes: should be flat packed by staff and students and either left
beside the bin for daily collection by the domestic staff to a dedicated waste bin
(RVH) or placed into the blue dumpster in the waste disposal lift lobby (BCH). It is
not the duty of domestic staff to flat pack boxes.
 Paper: On RVH site waste paper may be recycled using the white ‘SITA’ bags
(section 15.1.13). The University currently does not recycle paper from BCH site.
 Batteries: Dispose of regular batteries into QUB battery tubes in ISCA or MBC.
Lithium batteries must be given to the BLO who will arrange disposal through
Planon.
 Equipment: Obsolete equipment may be recycled through the University.
15.1.13 Confidential Waste
All waste containing sensitive information must be shredded before disposal. This
includes information that could reveal the identity of samples, personal information about
staff and students, as well as documents for disposal that may contain purchasing card
information. White ‘SITA’ confidential waste bags are available from the Stationery
store or Laboratory Floor Managers or Chief Technician. Appropriately filled bags (do not
overfill) should be sealed and left in the designated areas for collection.
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15.2 Laboratory Waste Disposal Containers
15.2.1 ORANGE PLASTIC BAGS
DO’s and DON’TS
-
Must be suspended in the appropriate holder.
Must not be more than ¾ filled.
Must be inspected for holes/punctures and if necessary double bagged.
Sealed at point of production by twisting, swan-necking and securing with a traceable
tag. To be eventually contained in a wheeled bin for collection (NB: on BCH site
place bag for disposal into yellow dumpster with orange tag).
-
No uncontaminated paper of any kind.
No food or general rubbish.
IMPORTANT! No sharps or other items that could puncture the bag.
No free liquid or blood. No waste with a COSHH Hazard.
No cytotoxic waste e.g. agarous ethidium bromide or SYBR Safe gels.
-
For the disposal of
RVH SITE
- N/A
BCH SITE
- Uncontaminated gloves
- Packaging around samples including
sample bags
- Paper tissue used to clean work
benches, water spills etc where no
known hazardous spills have taken
place.
- Reagent containers that are too small
to be rinsed out but have no COSHH
risk
- Plate seals
- Foil
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15.2.2 SHARPS BOX WITH ORANGE LID
DO’S AND DON’TS
- Must be dated and signed on assembly
- Must not be more than ¾ filled
- Must be dated and signed when locked (BCH site: attach traceable black tag and
place into untagged yellow dumpster for collection – ensure dumpster is kept locked)
- Should be disposed of within 1 month of assembling.
- Must never be placed in a yellow bag
- No plastic disposables
- No cytotoxic waste
- No tissue paper
For the disposal of
RVH SITE
-
BCH SITE
No blood contaminated sharps
N/A
Needles.
Syringes.
Disposable sharp instruments.
Contaminated broken glass e.g. whole
or broken slides.
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15.2.3 SHARPS BOX WITH PURPLE LID
DO’S AND DON’TS
-
Must be dated and signed on assembly
Must not be more than ¾ filled
Must be dated and signed when locked
Should be disposed within 1 month of assembling
- Must never be placed in a yellow bag
- No non-hazardous waste
- No non-hazardous plastic disposables
- No non-hazardous tissue paper
For the disposal of sharp equipment that have come into contact with cytotoxic
material or blood
RVH SITE
BCH SITE
Needles
Syringes
Disposable sharp instruments
Broken glass e.g. whole or broken
slides
- Ethidium Bromide Gels
- Sharps or pastettes contaminated with
blood
- Pipette tips
- Needles, scalpels and other sharp
disposable instruments
- Sharps or pastettes contaminated with
blood
- Disposable injector from OT2
- Drug medication
- Picogreen reagent, Qubit reagent,
SYBR Safe and gels, PeqGreen, E-gels
- Contaminated plates, tubes, containing
picogreen reagent, Qubit reagent or
other COSHH hazard.
-
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15.2.5 YELLOW RIGID CONTAINER WITH YELLOW LID
DO’S and DON’TS
- Must be dated and signed on assembly.
- Must not be more than ¾ filled.
- Must be dated and signed on locking.
- Must never be placed in a yellow bag.
- Not to be used as replacement sharps boxes.
This container is primarily for the containment of “free liquid” clinical waste:
RVH SITE
BCH SITE
- Blood containers
- Urine containers
- Saturated materials, tissue paper,
swabs
- Soiled material
- Waste pretreated by Trigene
- Waste with a COSHH risk e.g.
Taqman, ELISA
- All non sharp blood waste including
blood samples and solidified blood
waste.
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15.2.6 YELLOW RIGID CONTAINER WITH PURPLE LID
DO’S and DON’TS
-
Must be dated and signed on assembly.
Must not be more than ¾ filled.
Must be dated and signed on locking.
Non-sharp cytotoxic waste for incineration
Non-sharp waste with COSHH hazard for incineration.
- Must never be placed in a yellow bag.
- Not to be used as replacement sharps boxes.
This container is primarily for the containment of “free liquid” clinical waste:
RVH SITE
- N/A
BCH SITE
- Non-sharp lab waste that is cytotoxic,
-
has a COSHH hazard or that is
potentially hazardous.
Gels containing SYBR Safe or
PeqGreen. E-Gels.
All contaminated reaction plates,
gloves, tissue paper etc.
Small reagent containers.
Paper tissue and gloves from cleaning
up hazardous spills.
Labcoat contaminated by cytotoxic
material.
EZ DNA bisulfite treatment kit waste.
Non sharp waste contaminated with
drug medications.
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15.2.6 CLEAR AUTOCLAVE BAG
DO’S and DON’TS
-
Must be suspended in the appropriate holder
Must not be more than ¾ filled
Sealed at point of production by twisting, swan-necking and securing with
autoclave tape. To be eventually be transferred into a yellow biohazard bag with
traceable tag and contained in a wheeled bin for collection
-
No uncontaminated paper of any kind
No food or general rubbish
No Yellow or Blue tips
This container is primarily for the containment of “free liquid” clinical waste:
RVH SITE
-
-
Disposable plastic containers
Elisa Plates
Contaminated tissue paper
Tissue Culture flasks
BCH SITE
- N/A
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15.2.7 DOMESTIC WASTE
RVH – blue bag
BCH – clear bag
DO’S AND DON’TS
- No clinical waste at all
- No glass broken/unbroken
- Large boxes should be flattened and left outside the lab for removal (RVH only)
This bin is cleared by the cleaner and for their health should only contain:
RVH SITE
-
Domestic waste
Paper towels
Packaging
Polystyrene boxes
Uncontaminated gloves
The blue bag may be placed in a waste bin
rather than a holder
BCH SITE
- Packaging from inside delivery boxes
- Paper that is not confidential
- Plastic containers e.g. TE, EDTA
buffer bottles rinsed out three times
with tap water
- Paper towels
- Jiffy bags
- Uncontaminated plastic bags
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15.2.8 BROKEN GLASS BOX
DO’s and DON’TS
- All non-contaminated glassware
For disposal of:
RVH SITE
-
Sterilized glass broken/unbroken
Crockery
Batteries
Aerosols
BCH SITE
- Broken/ unbroken glass
- Small glass universal bottles
- If glassware previously contained
chemicals, rinse out carefully 3 times
before disposal
- Crockery
- Batteries
- Aerosols
Place into black dumpster for disposal
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Section 16: Procedures for Specimens
16.1 New Studies
All new studies must be reported to the Chief Technician who will retain details of
storage, freezer number and location, RVH site.
16.2 Human Tissue Act:
The substantive provisions of the Human Tissue Act 2004 came into force on 1
September 2006. In order to ensure compliance with the licencing requirements the
University has developed procedures which have implications for staff involved in the
removal, storage and use of human tissues and organs.
 Storage and recording of HTA relevant samples must comply with the HTA
guidelines and the Ethical Committee approved study specific protocol.
 The storage and recording of samples that do not come under the remit of the
HTA will follow the Ethical Committee approved study protocol.
The act does not necessarily apply to everyone. If you are intending to store certain
tissue types eg; whole blood, buffy coat samples, urine, sputum, tissue etc you will need
to comply with the Act. For more information please contact the Principal Investigator
(PI) for your study who will provide you with training in HTA procedures. For further
information and queries please contact our designated HTA adviser, Dr Ann McGinty.
For further advice please see Appendix G and the following website:
www.opsi.gov.uk/acts/acts2004/20040030.htm
16.3 Sample Collection
Venepuncture training must be undertaken by staff and students involved in studies
requiring blood draw from volunteers or study participants.



All users must ensure they have adequate training in phlebotomy.
Users must ensure that there is ethical permission and consent in place before
using the blood room facility.
It is the responsibility of the phlebotomist to ensure all proper Health and Safety
measures for venepuncture are followed.
16.4 Sample Reception
Specimen reception: specimens arriving into the lab must be anonymised (read section
3.2.2.2).
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16.5 Sample Storage
16.5.1 Ultra low temperature storage:
All samples for ultra low temperature storage must be stored in suitable vials. These
should not be more than ¾ filled to allow for expansion of the sample during
freezing. Screw cap closures are preferred.
Vials must be clearly labelled with the sample identifier. A printed or handwritten label
capable of withstanding ultra low temperatures may be used. Alternatively vials with a
writing area are acceptable. A black freezer-proof pen should be used for hand writing
any label.
Sample vials should not be stored in open racks in the freezer. They should be stored in
clearly labelled boxes with lids or in bags to prevent spillage.
Liquid samples should preferably be frozen upright.
RVH Site: Do not use polystyrene racks as this delays the freezing process.
BCH Site: DNA samples may be stored in polystyrene racks but avoid using if possible
as they are bulky and take up a lot of space in the freezer.
16.5.2 Refrigeration at 4oC Storage:
RVH Site: Samples to be stored at 4oC should be stored in a cold room as this is
temperature monitored and alarmed. Samples should only be kept in a refrigerator
during the working day to facilitate laboratory analysis.
BCH Site: DNA samples may be stored in the fridge for short periods of time to avoid
frequent freeze thawing. Due to the risk of evaporation of liquid, storage in the fridge is
not recommended unless absolutely necessary. Deep well storage plates containing
DNA ‘stock’ or DNA ‘working stock dilution’ should not be stored in the fridge for any
longer than 24 hours. When in the fridge, the plates must be securely sealed and
parafilmed.
Vials and tubes must be clearly labelled with the sample identifier. A printed or
handwritten label in black permanent pen is acceptable. Alternatively vials or tubes with
a writing area are acceptable.
BCH Site only: IMPORTANT! → In Post-PCR there are two fridges. One is ‘clean’ for
storage of reagents only and the other is for Post-PCR products. Do not put PCR
products into the reagent fridge.
16.6 Transfer of samples to other locations
16.6.1 Material Transfer Agreement (MTA)
IMPORTANT! → An inventory is kept of freezer contents. You must consult with your
Floor Manager or Chief Technician if you intend to move samples from one freezer to
another or sending to another laboratory, so that records can be updated.
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IMPORTANT! → HTA Relevant Material sent to a third party is governed by an
outgoing Material Transfer Agreement (MTA).
BCH Site only: DNA samples may not be transferred to an external location
without the approval of Prof. Peter Maxwell and an MTA must also be in place.
Receipt of relevant material is governed by an incoming MTA.
16.6.2 Sample Transport
Samples must be packaged correctly for transport to other locations. Full details are on
the S drive.
A suitable transport case should be used for transfer between clinics and laboratories or
local locations. Suitable packaging can be obtained from suppliers such as Sarstedt.
Information on the transport of samples by post and courier can be found on the Safety
Service web site: www.qub.ac.uk/so
Additional information can be found at:
http://www.hse.gov.uk/aboutus/meetings/committees/acdp/080609/acdp-92-p5g.pdf
16.6.3 Protocol for shipping samples in dry ice by courier:
Samples requiring dry ice shipment must only be prepared for transport by staff who
have completed the approved training course. The shipment of biological specimens on
dry ice by courier air shipment must comply with the IATA Dangerous Goods
Regulations. Details can be found on the Safety Service web site www.qub.ac.uk/so.
Also see the UNECE website for detailed information on ADR 2005.
www.unece.org/trans/danger/publi/adr/adr2005/05ContentsE.html.
Specific protocols are in place for the shipping of samples in dry ice. The Laboratory
Procedure must be read and signed before samples are shipped.
16.6.3 Transport of Dangerous Goods
The transport or shipment of substances classified as potentially hazardous during
transport (dangerous goods) is regulated under national and international regulations.
Only staff with a certificate of training may consign dangerous goods for transportation.
For general dangerous goods for transport by road and sea, Mrs Jackie O'Connor,
School of Chemistry and Chemical Engineering, (jackie.oconnor@qub.ac.uk, Ext 4673),
can advise on the classification, packaging and labelling of goods.
16.7 Sample disposal
HTA guidelines for the recording of sample disposal should be followed where applicable
and recorded on the QUB online HTA database.
Study guidelines for the recording of sample disposal should be followed where
appropriate.
Sample disposal should follow the waste disposal guidelines referred to in Section 15 of
this handbook.
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INDEX
Abbreviations
Access and Security
Accident Reporting
Backing up sensitive information
Bicycle Security Advice
BLO/Buildings Liaison Officer
Car Parking Security
Carcinogens
Chemical Hazard Symbols
Compressed Gases
Confidential Helpline
Controlled Substances
COSHH
CPH H&S Arrangements
CPH Development Plan
CPH H&S Committee Membership
CPH H&S Committee Terms of Reference
CPH Health and Safety Policy
CPH Health and Safety Structure
Data Handling
Disability
Disinfection
Document History
Electrical Safety
Email
Emergency telephone numbers
Eye and Face Protection
Fire Fighting Equipment
Fire Prevention and Control
Fire Wardens
First Aid
First Aid
Footwear in the Laboratory
Fume Cupboards
General Health and Safety
Glassware
Good Housekeeping
Hazard Signs
HTA
HTA Compliance
Hygiene for Laboratory Users
3
30
66
27
31
41
32
56
39-40,54
84-85
28
22
77-78
18-22
23
17
16
13
15
25
20
61
2
58
27
10-11
52
32
67-69
70
20,29
62-65
53
45-46
47
60
48
38
102
21
49
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Index of contents
Infectious Agents and GMO’s
Introduction
Laboratory Procedures/LP/SOP
Laboratory Records
Laboratory Samples
Laboratory Waste Disposal
Lifting and Handling
Lone Working Policy
Major Incident Reporting
Noise in the Workplace
New and Expectant Mothers
Occupational Stress
Off-site Digital Analysis and Storage
On-site Digital Analysis and Storage
Permit to Work outside normal working
hours
Personal Security
Pregnancy/New and Expectant mothers
Protective Gloves and Glove Choice
Radiation Safety Procedures
Reporting a Security Breach
Reporting of Faults/BLO
Respiratory Protection
Risk Assessments
Sample Storage
Sample Transport
Sharps/Needlestick injury
Smoking
Spillages
Strategic Risk Register
Terms of Reference/CPH H&S Committee
Tidiness
Tissue Culture
Transfer of Samples/MTA
University Health and Safety Policy
University Health and Safety Structure
Use of Hazardous Chemicals
Visitors
Waste Disposal/ Laboratories
Wellbeing
Workplace Inspections
4-8
21
9
82
27
26,102
86
59
33
22
41
20, 28
28
27
27
33
31
20, 28
50,51
83
27
41
53
79
103
104
60,65
29
71-76
22
16
28
57
103
12
14
54
20, 40
86
28
18,48
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