Introduction to Microbiology Lab
Biol 132L (1 credit hour)
Dr. Kathryn Sutton, Assistant Professor of Biology
Spring, 2007
Tuesdays 8-10:50, CBH 227
General Information
Office Location
Office Phone
Email
Mail Station
Office Hours
CBH 222
588-6526 (or 6526, if on campus)
kathryn.sutton@clarke.edu
MS 1791
MW 10-11
W 2-4
Th 11-12
F 10-12, 2-3
Or by appt. (please call or email me)
Required Text
Morello A, Mizer H, and Granato P. Microbiology, Applications to Patent Care, 8th ed.
Boston: McGraw-Hill, 2003
Course Description
Introduction to Microbiology Lab gives students “hands-on” experience in Microbiology.
Emphasis will be on relating lab exercises to patient care and diagnosis of infectious
disease.
Course Goal and Statement Connecting goals to the Clarke College Mission:
Students who complete this course will have a broad understanding of Microbiology and
how this field relates to human health. Because this course is designed for Nursing
majors, moral issues within the medical fields and compassion for the ill will also be a
focus, in conjunction with the mission statement of the college.
1
Expectations
Attendance: You are expected to attend all labs. There is no opportunity to make up labs.
If you cannot attend a lab, you may complete those assignments that you can; however, you
cannot get credit for in-class assignments (quizzes included). There are many
opportunities to earn points for your lab grade. A single absence (due to funeral
attendance or illness) should not impact your grade if you regularly attend all labs and
study for your weekly quizzes. Repeated absences and/or lateness will impact your
grade in a negative way and you have no one to blame but yourself.
Preparedness: You must prepare for each lab by reading the assigned pages in the lab
manual or text and familiarizing yourself with the key terms for the lab.
Assignments: All assignments must be turned in at the beginning of the lab on the day
they are due. There are no exceptions to this rule. Any assignments due the day you
miss class must be given to me prior to the start of class (via email in WORD or copied
into the body of the email or delivered by a classmate). This rule stands no matter
what reason you have for missing lab. 10% will be deducted right off the top of the
assignment for late work. 10% will also be deducted for each addition late day.
After 4 days, do not bother to turn the assignment in to me.
Lab Safety: You must follow at all times the safety procedures outlined in your lab manual.
Participation: You are expected to participate fully in each lab. I, as the instructor,
reserve the write to deduct points from your individual grade if I suspect that you are not
pulling your own weight in group assignments and lab work. If you sleep during lab, you
will be asked to leave. Repeat sleepers will be asked to withdraw from the course.
Learning and fun: Hands-on is the best way to learn science. If you have a good attitude,
you will succeed!!
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Course Outcomes-Students
will successfully….
Proper use and care of the
microscope.
Activity/Project/
Performance
Assessed by the instructor
during the first three weeks of
class
Program Outcomes
Successful completion of the
questions assigned for each lab
exercise.
Students will demonstrate the
ability to communicate
scientific concepts and findings
in writing
Students will demonstrate
knowledge of structure and
function of cells
Students will correctly use
technology in the lab
Sterile technique and handling
of live cultures.
Follow procedures for staining
bacteria.
Apply classroom knowledge to
patient care.
Define and properly use terms
that are critical to
microbiologists.
Demonstrate understanding of
means by which microbes grow
and how that growth can be
controlled.
Weekly quizzes on key terms.
Lab exercises, quizzes and
homework.
Students will demonstrate the
ability to communicate
scientific concepts and findings
in writing
Students will demonstrate
knowledge of structure and
function of cells
Statement on American with Disabilities Act
Any student who needs accommodations must contact Myra Benzer in the Learning Center.
Be sure this happens very early in the semester. The Learning Center is located in the
upper level of the Clarke College Library. You will not be able to take exams in the
Learning Center unless there is a documented reason for doing so.
Statements on Academic Integrity
Clarke College Statement:
Although course activities encourage collaboration and shared learning, acknowledging
others for their contribution is crucial. Do not copy words, ideas, papers or parts of
papers from any source without giving credit through acceptable forms of documentation.
Do not lend out your ideas, papers, or parts of papers to others. Passing off someone
else’s work as your own or allowing your work to be used this way is a serious break in the
academic integrity of this class and the college. It may result in failure or expulsion. (See
student handbook on academic integrity).
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Dr. Sutton’s Statement:
Cheating and Plagiarism will not be tolerated in any form. This includes tests, quizzes,
homework assignments, and group projects and will result in a zero for the test or
assignment. It is considered cheating if you fail to cite your references (internet sites
included). It is never acceptable (and you will fail the assignment if you do this) to
copy ANYONE’S work word for word. You must always use your own words and then
credit your source, using an acceptable and consistent style format. I will not accept
work that does not have a bibliography.
Grade Assignment
The following is the basis for your grade:
Quiz or Assignment
10 quizzes (10 points each)
Homework assignments
(34 questions total, 5 points each)
Results table p. 148
Proper use and storage of microscope
Point Value
100
170
10
20
Your grade will be based on the percentage of the total number of points that you earn
throughout the semester.
Points earned
279+
270-278
261-269
249-260
240-248
231-239
219-230
210-218
201-209
189-200
180-188
179 and less
Out of
300
300
300
300
300
300
300
300
300
300
300
300
Percentage
93+
90-92
87-89
83-86
80-82
77-79
73-76
70-72
67-69
63-66
60-62
59 and below
Letter Grade
A
AB+
B
BC+
C
CD+
D
DF
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Tentative Lab Schedule (instructor reserves the right to make changes, if necessary)
Date Exercise # and Description
Pre-lab Assignment and
Quiz
1/16
Introduction to Course
None
Review of Lecture and Lab Syllabi
Lab Safety and procedures
1/23
#1 Microscope
Quiz 1
#4 Simple Stain
Quiz 1
#11 Culturing from the environment
Read lab 11
Read lab 3 (p. 26 and section 3.2)
1/30
#3 Wet Mount-demonstration
#5 Gram Stain
Quiz 2 on key terms
# 9 Streaking to pure culture from environment
Read lab 9
plate (you will need to come to lab 3 additional times over
Post-lab Assignments
None
p. 36 # 4,6,8 Due:
discuss results next week
p. 31 #1 Due:
p. 41 #4,7,8,9 Due:
p. 63 #3 Due:
the next 2 weeks to obtain pure culture)
#2 Handling cultures
2/6
2/13
2/20
2/27
3/6
3/13
#7.1 Endospore staining
DNA-RNA-Protein (working with models)
Review for Lecture Exam I
Lecture Exam I in lab@ 9 AM
Quiz 3 on key terms
Quiz 4 on key terms
Gram, and Endospore stain pure culture from
environmental plates; make storage slant
Lecture on factors that influence microbial growth
Oxygen and temperature influence microbial growth
#28 Anaerobic bacteria
No Lab-winter Break
Lecture on next week’s experiment @ 8 AM
Exam II following lecture
Review Labs 5 and 7.1
Quiz 5 on key terms
Read text p. 156-163
Read test p. 170-173, 178-
p. 23 #1,4, 5 Due:
p. 52 #4,6 Due:
Fill in worksheet prior to
leaving lab #1,2,3
Text p. 180 Review questions
# 3, 5 and MC questions # 3
& 4 (explain answers for MC) Due:
Text p. 180 Review questions
# 1,10 Due:
5
Day/
Date
3/20
3/27
4/3
4/10
4/17
4/24
Exercise # and Description
Bacterial growth (handout)
#13 The Autoclave
#16 Diagnostic Microbiology
#20 The Staphylococci
No Lab-Easter Break
Exam III in lab; begins @ 9 AM
#14 Disinfectants
The Effects of UV light-Handout
#15 Antibiotics: The Kirby-Bauer Method
Continue Staph isolation
Hand Washing Experiment (handout)
Effectiveness of Alcohol (handout)
Pre-lab Assignment and
Quiz
Quiz 6 on last week’s lecture
Read text p. 170-173, 178
Quiz 7 on key terms
Lecture
Post-lab Assignments
Quiz 8
p. 93 #5,6,7 Due:
p. 104 #4,10,11 Due:
Read handout
Read handout
Quiz 9 (read both handouts)
Discuss results in lab next
week
Copy the results table p. 148
p. 148 #1,4,8,9
Continue staph
5/1
#26 Culture Techniques- Urine
Case Study III assigned
Latex agglutination of Staph
Examine Urine Culture plates
Case study presentations
Text p. 180 Review questions
# 1,10 Due:
p. 86 #6,9 Due:
Lab manual p.132; & Fig. 19.1
Quiz 10
6