Environment
Analysis of aldehydes
8-
97
Simultaneous analysis of DNPH-derivatized aldehydes
Wakopak® Wakosil DNPH
Analysis of 16 DNPH-aldehydes 〈Separation of n- and iso-butyladehyde by HPLC〉
Flow Rate
：Wakopak® Wakosil DNPH
（4.6 mm × 150 mm）
：A）Wakosil® DNPH Eluent A
B）Wakosil® DNPH Eluent B
0-16 min. B;10%
16-35 min. B;10-90%
35-45 min 90%
：0.6 mL/min. at 37℃
Detection
：UV 360 nm,0.016 AUFS
Column
1
2
Eluent
4
3
8
5
6
9
10
11 12
16
13
7
15
14
0
5
10
15
20
25
30
35
40
Wakopak Wakosil DNPH-Ⅱ
Rapid analysis of 15 DNPH-aldehydes
Sample：1）Formaldehyde-2,4-DNPH
2）Acetaldehyde-2,4-DNPH
3）Propionaldehyde-2,4-DNPH
4）Acrolein-2,4-DNPH
5）Acetone-2,4-DNPH
6）
iso -Butyralde-2,4-DNPH
7）
n -Butyraldehyde-2,4-DNPH
8）Crotonaldehyde-2,4-DNPH
9）
iso -Valeraldehyde-2,4-DNPH
10）
n -Valeraldehyde-2,4-DNPH
11）Benzaldehyde-2,4-DNPH
12）Hexaldehyde-2,4-DNPH
13）
o -Tolualdehyde-2,4-DNPH
14）
m -Tolualdehyde-2,4-DNPH
15）
p -Tolualdehyde-2,4-DNPH
16）2,5-Dimethylbenzaldehyde-2,4-DNPH
each 0.625 μg/mL（as aldehyde,ketone）
®
mAU
5.0
mAU
1
4.0
3.0
mAU
2
14
1
12
12
10
10
8
8
2.0
6
After elution of one Presep -C
DNPH with acetonitrile, the
eluate was adjusted to 5 mL
with acetonitrile.
R
4
2
2
1
0.0
5
3
2
7
14
0
2
4
6
8 10 12 14 16 18
Time(min)
4
35
30 L of air in the laboratory
were taken with Presep R -C
DNPH at a flow rate of 0.5
L/min, and the components
were eluted as stated above.
7
6
0
-2
0
2
4
4
2 Example of analysis of
indoor air sample
1
6
3
1.0
1 Cartridge blank
3
14
10
9 11
8 12 14
13 15
3 Reference standard
-2
0
2
4
6
8 10 12 14 16 18
Time(min)
0
2
4
■Solid-phase extraction cartridges
Product Name
Presep®-C DNPH（Short）
Presep®-C DNPH
Presep®-C Ozone Scrubber
6
8 10 12 14 16 18
Time(min)
Chromatogram of reference
standards containing 15
DNPH-derivatized aldehydes
Grade
for Sample Pretreatment
for Sample Pretreatment
for Sample Pretreatment
■HPLC columns
Product Name
Size
Wakopak® Wakosil DNPH
4.6 mm × 250 mm
Wakopak® Wakosil DNPH-Ⅱ
4.6 mm × 150 mm
Grade
D
W
D
W
Sample：
1) Formaldehyde-2,4-DNPH
2) Acetaldehyde-2,4-DNPH
3) Acetone-2,4-DNPH
4) Acrolein-2,4-DNPH
5) Propionaldehyde-2,4-DNPH
6) Crotonaldehyde-2,4-DNPH
7) n-,iso -Butyraldehyde-2,4-DNPH
8) Benzaldehyde-2,4-DNPH
9) iso Valeraldehyde-2,4-DNPH
10) n-Valeraldehyde-2,4-DNPH
11) o-Tolualdehyde-2,4-DNPH
12) m-Tolualdehyde-2,4-DNPH
13) p-Tolualdehyde-2,4-DNPH
14) Hexaldehyde-2,4-DNPH
15) 2,5-Dimethylbenzaldehyde-2,4-DNPH
0.1 μg/mL each (as aldehyde,ketone)
Pkg.Size
20 pcs.
20 pcs.
20 pcs.
Wako Cat. No.
291-43951
290-34251
293-40351
Pkg.Size
1 pcs.
1 pcs.
1 pcs.
1 pcs.
Wako Cat. No.
238-59411
234-59413
231-61731
237-61733
■Eluents
Product Name
Wakosil® DNPH Eluent A
Wakosil® DNPH Eluent B
Wakosil® DNPH-ⅡEluent A
Wakosil® DNPH-ⅡEluent B
Grade
for HPLC
Acetonitrile
for Aldehyde Analysis
Pkg.Size
1L
1L
1L
1L
100 mL
200 mL
Wako Cat. No.
233-01611
230-01621
236-02181
233-02191
017-17743
011-17741
■Standard Solutions
Product Name
Specification
Volume Wako Cat. No.
16 Aldehydes-DNPH Mixed Standard Solution (10 μg of each aldehyde/mL of acetonitrile solution)
1. Formaldehyde-DNPH, 2. Acetaldehyde-DNPH, 3. Propionaldehyde-DNPH, 4. Acrolein-DNPH, 5. AcetoneDNPH, 6. iso-butylaldehyde-DNPH, 7. n-butyladlehyde-DNPH, 8. Crotonaldehyde-DNPH, 9. isovaleraldehyde-DNPH, 10. n-valeraldehyde-DNPH, 11. Benzaldehyde-DNPH, 12. n-hexanal-DNPH, 13.
o-tolualdehyde-DNPH, 14. m-tolualdehyde-DNPH, 15. p-tolualdehyde-DNPH, 16. 2,5-dimethylbenzaldehydeDNPH
for HPLC
1 mL× 5 A 018-18231
6 Aldehydes-DNPH mixed Standard Solution (0.1 μg of each aldehyde/μL of acetonitrile solution) for Stinking Substances
2 mL× 5 A 012-15451
Analysis (HPLC)
1. Formaldehyde-DNPH, 2. Acetaldehyde-DNPH, 3. Propionaldehyde-DNPH, 4. iso-butylaldehyde-DNPH,
5. n-valeraldehyde-DNPH, 6. iso-valeraldehyde-DNPH
2 Aldehydes-DNPH mixed Standard Solution (0.1 μg of each aldehyde/μL of acetonitrile solution)
1. Formaldehyde-DNPH, 2. Acetaldehyde-DNPH
for Air Pollutant
Analysisi (HPLC)
2 mL× 5 A 012-17391
Wako Product Information − Chromatography − 8th Edition
〈9th-107〉
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