1. Living organisms are made up of one or more cells. 2. Both animal and plant cells have a cell membrane, a nucleus and a cytoplasm. 3. In addition, plant cells also have a cell wall, a vacuole and chloroplasts. chloroplasts cell membrane nucleus vacuole cell wall cytoplasm Animal cell Cell structure nucleus cytoplasm cell membrane cell wall vacuole chloroplast Plant cell Function Controls all of the cell’s activities and passes on genetic information from one generation to the next The site of biological processes and biochemical reactions e.g. respiration. Controls movement of materials into and out of the cell. Provides support to the plant cell, and helps the plant maintain rigidity. Site of water and solute storage, and maintains water balance through osmosis. Absorbs light energy required in photosynthesis. Living cells summary booklet page 1 4. Cells are used commercially and in industries. Examples of such uses are: i. Bread making – when yeast respires anaerobically, carbon dioxide gas is produced. Carbon dioxide bubbles get trapped in the dough causing the bread to rise. ii. Alcohol production – during alcoholic fermentation yeast produce alcohol as a waste product. iii. Antibiotic production – fungi are used to kill bacteria. These are known as antibiotics e.g. penicillin. Some bacteria do show resistance to some antibiotics though. iv. Yoghurt production – Some bacteria are naturally present in milk and respire anaerobically as they grow, producing lactic acid. Lactic acid causes milk to curdle, thus producing yoghurt. v. Alternative fuel production – Alcohol produced through fermentation of sugar cane can be mixed with petrol and used as fuel. Methane produced by anaerobically respiring bacteria can be used to produce biogas. Living cells summary booklet page 2 1. Diffusion is the movement of the molecules of a substance from a region of high concentration to a region of low concentration, down a concentration gradient, until both concentrations become equal. This process can be seen below with the black molecules diffusing through the clear molecules. Equal concentration of black molecules throughout High concentration of black molecules 2. A number of substances enter and leave a cell by diffusion e.g. oxygen, carbon dioxide, glucose, amino acids, waste products. amino acids carbon dioxide oxygen waste products glucose 3. Diffusion is extremely important in cells to: i. remove and reduce build up of waste products Living cells summary booklet page 3 ii. supply raw materials for respiration and photosynthesis 4. Osmosis is a special case of water diffusion. Osmosis is the movement of water from an area of high water concentration to an area of low water concentration, down a water concentration gradient, and through a selectively permeable membrane. 5. Cell membranes are known as selectively permeable membranes, because they contain tiny pores that only allow small molecules e.g. glucose, water, oxygen through, and do not allow larger molecules e.g. starch, proteins through. 6. Osmosis is important to living cells, allowing water to pass between cells and either nearby cells or the surrounding environment. For example, water movement in plants is as follows: i. soil solution (high concentration) → root hairs (low concentration) ii. xylem vessels (high concentration) → stem cells (low concentration) iii. xylem vessels (high concentration) → green leaf cells (low concentration) 7. If animal or plant cells are placed in an hypotonic solution (a solution with a higher water concentration) e.g. pure water, the cells will gain water by osmosis. 8. If animal or plant cells are placed in an hypertonic solution (a solution with a lower water concentration) e.g. 1.0M sucrose solution, the cells will lose water by osmosis. 9. If animal or plant cells are placed in an isotonic solution (a solution with the same water concentration), there will Living cells summary booklet page 4 be no loss or gain of water – no osmosis (water movement) will occur. 10. Animal and plant cells react differently to hypoand hypertonic solutions due to the differences in these cells. Remember plant cells contain a cell wall and vacuole that animal cells do not. Cell type animal animal animal plant plant plant Solution type hypotonic isotonic hypertonic hypotonic isotonic hypertonic Effect on the cell cell bursts cell remains unchanged cell shrinks cell becomes turgid cell remains unchanged cell becomes plasmolysed 11. Plant cells do not burst when they take up water due to presence of a slightly stretchy cell wall. Plant cells become turgid because the vacuole becomes filled with water and expands pushing the cytoplasm against the cell wall. 12. When plant cells lose water they become plasmolysed, which means the cell contents lose water and pull away from the cell wall. 13. When plant cells lose water, shrink in size and become soft, they are described as being flaccid. Living cells summary booklet page 5 1. A catalyst is a substance that: i. lowers the energy input needed for a chemical reaction to go ahead. ii. speeds up the rate of a chemical reaction. iii. take part in the chemical reaction but remain unchanged. 2. Enzymes are biological catalysts, which are made by all living cells. 3. Enzymes are made of protein and are essential for the functioning of all living cells. 4. The substrate is the substance on which the enzyme acts e.g. starch is the substrate for amylase. 5. Each enzyme only acts on one substrate, thus enzymes are therefore termed “specific” e.g. the enzyme amylase is specific to the substrate starch. 6. The shape of the enzyme is complementary to the substrate, thus the substrate is able to fit exactly into the active site of the enzyme. active sites products + + enzyme substrate Living cells summary booklet Enzyme-substrate complex enzyme page 6 7. Degradation is the enzyme-controlled breakdown of a large complex substance into smaller molecules. An example of a degradation reaction: starch amylase (substrate) maltose (product) 8. Synthesis is the enzyme-controlled build-up of a large complex molecule from smaller molecules. An example of a synthesis reaction is: phosphorylase glucose-1-phosphate (substrate) 9. starch (product) Two factors that affect the activity of an enzyme are: i. temperature ii. pH 10. The “optimum” temperature or pH for an enzyme is the temperature or pH at which it works best, thus has the greatest activity. 11. as follows: i. ii. iii. The effect of temperature on enzymes is At very low temperatures (0-10°C) enzyme activity is very low, and therefore the rate of the reaction is very slow. As temperature increases to about 40°C the enzyme activity and rate of reaction also increase reaching a maximum at the optimum temperature of 40°C. At temperatures greater than 40°C the enzyme activity and rate of reaction decrease rapidly, reaching 0 between 50 and 60°C. Over 50°C enzymes no longer work because their structure becomes altered and the shape 12. Living cells summary booklet page 7 active site is no longer complementary to the shape of the substrate. Enzymes in this state are said to be denatured. 13. Most enzymes work within the range of pH5-pH9, with an optimum pH of 7 e.g. amylase. 14. i. ii. Living cells summary booklet Some exceptions include: pepsin which works in the stomach, working under acidic conditions, with an optimum pH of 2.5 alkaline phosphatase is involved in bone formation and has an optimum pH of 10 page 8 The chemical energy stored in glucose is released by a series of enzyme-controlled reactions called respiration. i. ii. Some energy is released as heat from cells during respiration. iii. Most of the energy released is used for cellular activities such as: i. muscle contraction ii. cell division iii. synthesis of proteins iv. transmission of nerve impulses iv. The energy released from respiration (breakdown) of glucose is used to synthesise adenosine Triphosphate (ATP) from adenosine diphosphate (ADP) and phosphate (Pi). adenosine Pi Pi + Pi + energy (from respiration of glucose) enzyme adenosine Pi Pi Pi v. This ATP contains a high level of chemical energy, and is thus an immediate and rapid source of energy for a variety of activities in living cells e.g. muscle contraction. Living cells summary booklet page 9 vi. Energy is released when ATP is broken back down into ADP and Pi. adenosine Pi Pi bond which must be broken to release energy enzyme adenosine Pi Pi Pi + Pi + energy (for cellular activities) Glycolysis is the process where glucose is broken down into pyruvic acid, thus yielding 2 molecules of ATP per molecule of glucose. 7. 8. This pyruvic acid is then broken down further. The end products of breaking down pyruvic acid depends on whether oxygen is present or absent. aerobic respiration need for oxygen glycolysis total energy yield end products yes anaerobic anaerobic respiration in respiration in animals plants no no yes – 2ATP 38ATP yes – 2ATP 2ATP yes – 2ATP 2ATP carbon dioxide and water lactic acid ethanol and carbon dioxide Under anaerobic conditions in animal cells, pyruvic acid is broken down into lactic acid in 9. Living cells summary booklet page 10 a reversible process. This means that when oxygen becomes available, repaying the oxygen debt, the lactic acid is converted back into pyruvic acid. 10. Lactic acid build-up in muscle cells causes muscle fatigue. Under anaerobic conditions plants and yeast convert pyruvic acid to ethanol and carbon dioxide in an irreversible process since the carbon dioxide is lost. 11. Living cells summary booklet page 11 1. Photosynthesis is a series of enzymecontrolled reactions which allow green plants to make their own food. Plant cells have structures called chloroplasts, which contain chlorophyll. 2. It is this chlorophyll that captures light energy from the sun and converts it into chemical energy in the form of ATP. 3. This ATP is used in the production of 4. glucose. 5. Photosynthesis can be summarized in the following equation: carbon water sunlight glucose + oxygen + dioxide chlorophyll The carbon dioxide required for photosynthesis diffuses into the leaf from the air through the stoma (small pores on the surface of the leaf). Oxygen produced during photosynthesis diffuses out of the leaf through the stoma. 6. 7. ii. Photosynthesis has two stages: photolysis – process where energy from sunlight is used to split water molecules into hydrogen and oxygen. Living cells summary booklet page 12 iii. 8. i. ii. iii. carbon fixation – process in which carbon dioxide from the air is combined with hydrogen to make glucose. During photolysis: oxygen is released into the air as a by-product ATP is made from ADP + Pi – this ATP is passed onto and used during the carbon fixation stage. Hydrogen is released, picked up by a hydrogen carrier molecule and passed onto the carbon fixation stage. 9. Carbon fixation involves a number of enzyme-controlled reactions which use the ATP produced during photolysis. 10. The glucose produced during photosynthesis can be turned into: i. starch – a storage carbohydrate composed of 200-800 glucose molecules forming a chain e.g. plant food store glucose unit starch molecule ii. cellulose – a structural carbohydrate composed of 1000-1500 glucose molecules forming a linear shape e.g. building material present in plant cell walls. glucose molecule Living cells summary booklet page 13 cellulose A limiting factor in photosynthesis is anything which, when in short supply, reduces or limits the rate of photosynthesis taking place e.g. i. light intensity ii. temperature iii. carbon dioxide concentration 11. 12. Horticulture is the cultivation of plants especially in gardens and green houses. 13. In horticulture, limiting factors are removed by use of: ii. supplementary lighting iii. carbon dioxide enrichment iv. heating in order to increase crop yields. Living cells summary booklet page 14