FINAL EXAM : Microbiology 480
1. A ribozyme is a a. protein b. RNA c. DNA d. Peptide e.
2. An Intein is a piece of a. virus b. RNA c. DNA d. Peptide
3. In the tryptophan operon, the synthesis of the leader protein results in a. Termination of transcription b. Initiation of transcription c. Induction of a activator protein
None (1)
4. The function of the kinase in signal transduction regulation is a. methylation b. hydrolysis c. phosphorylation
5. Single stranded RNA viruses may form mRNA in a host cell my a. direct use, no copying b. transcription transcription d. all of the above (1)
6. The enzyme luciferase requires a.ATP b.Light c.Sugars d.oxygen e.None (1)
7. Bacteriorhodopsin is found in a. cyanobacteria

b. halophilic Bacteria d. methanogens and is involved in e. light-mediated carbon synthesis f. oxygen-mediated ATP synthesis g. light-mediated ATP synthesis h. oxygen-mediated carbon synthesis
8. F420 is found in all a. Archaea b. Bacteria c. Methanogens d. Methylotrophs e.
9. Thermoplasma and Mycoplasma both have a. Flagella b. Sulfur metabolism
(1) d. No cell wall e. A and D (1)
10. One organisms waste product is often another organisms substrate. The sulfide used as an electron donor by certain chemolithotrophs often arises as the metabolic product of reducers b. Methanogens d. Fermentative Gram + bacteria
11. Give brief definitions for 5 of the following: (2 points each) a. photoheterotrophy b. anoxygenic photosynthesis c. transposon d. prion e. operon f. PCR (10)

1
Name 3 lineages that are photosynthetic, and note whether they are oxygenic or anoxygenic phototrophs. (6)
Name some unusual feature of spirochetes. (2)
The archaeal domain is divided into 2 main kingdoms. What are they? (2)
Draw a detailed CLEAR diagram to show EITHER:
DNA replication in bacteria OR specialized trasduction
Be sure to explain the different steps or enzyme functions.
(10)

Explain the genetic regulation occurring in this operon. Say whether it is positive or negative regulation. Use a diagram to show how the operon in regulated. (10)

Graduate students only.
Write a brief essay on one of the following:
1. Mechanisms of genetic transfer in bacteria