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Genomic DNA
Genomic DNA Genomic DNA
Wizard®
Purification
Genomic Blood gDNA
Systems
ReliaPrep™
Wizard® ReliaPrep™
SV
Miniprep
DNA
gDNA
ReadyAmp™
Wizard®
MagneSil®
SV 96
Genomic
Miniprep
Wizard®
Wizard®
Maxwell®
16
Magnetic 96 Magnetic
Fixed Tissue DNA Plant
Food
MagneSil® ReliaPrep™
Large Volume
Blood
HT gDNA
Genomic
Isolation
High-Throughput
Single-Column
Specifications
Yields Up To (µg)
(1)
Varies
20
30
30
N/A
30
400ng
Varies(2)
Varies(1)
Varies(1)
9
300µg/
10ml Blood
Approximate Time
60minutes
30minutes 20minutes 45minutes
20minutes 30minutes 45minutes
30minutes
1 hour
30-40minutes 90minutes
<8hr/96(3)
Technology
Sol
Mem
Mem
Mem
Res
Mem
Mag
Mag
Mag
Mag
Mag
Mag
Manual/Automated
M
M
M
M
M
M/A
M
M/A
M
A
A
A
˜
˜
»
˜
˜
˜
˜
»
»
»
˜
»
˜
»
Sample Type
Blood
Buccal Swabs
˜
˜
˜
Dried Blood Spots
˜
Stool
˜
»
Animal Tissue
˜
˜
Bacteria
˜
Cells
˜
Ear Punches/Rodent Tail
˜
˜
»
˜
˜
˜
˜
˜
˜
˜
˜
˜
˜
Fixed Tissue (formalin, paraffin)
˜
˜
»
Insects
˜
»
Plant Tissue
˜
Yeast
˜
Cat No
A1120
»
»
»
Fungi
˜
»
˜
Food
˜
˜
»
A5081
A2360
A2051
A7710
A2370
MD1490
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, Sol:
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FF3760
FF3750
AS2000
MD1360
A1751
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Wizard® Genomic DNA Purification Kit
ReliaPrep™ Blood gDNA Miniprep System
ReadyAmp™ Genomic DNA
Maxwell® 16 System
MagneSil® Blood Genomic,Max Yield
ReliaPrep™ Large Volume HT gDNA Isolation System
< Wizard® Genomic DNA Purification Kit
< ReliaPrep™ gDNA Tissue Miniprep System
< Wizard® SV Genomic DNA Purification System
< Wizard® SV 96 Genomic DNA Purification System
< Maxwell® 16 System
< Wizard® Genomic DNA Purification Kit
< Wizard® SV Genomic DNA Purification System
< Wizard® SV 96 Genomic DNA Purification System
< Maxwell® 16 System
< Wizard® Genomic DNA Purification Kit
< Wizard® Magnetic 96 DNA Plant System
< Maxwell® 16 System
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< Wizard® Genomic DNA Purification Kit
< MagneSil® Genomic, Fixed Tissue System
< Maxwell® 16 System
< Maxwell® 16 System
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PCR analysis of genomic DNA isolated from whole blood.
Lane M: 1kb DNA Ladder
Lane 1:no dna control (–)
Lane 2:positive control (+)
Lanes 3, 5, 7 and 9: samples rehydrated at room temperature for 20 minutes.
Lanes 4, 6, 8 and 10: samples rehydrated at 4°C overnight.
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Proteinase K
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A1120
100 isolations
A1125
500 isolations
V3125
100mg
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Primate Cell Lines
Rodents
Gram(-) Bacteria
Gram(+) Bacteria
Yeast
Human Blood
Insect cells
Plant Leaf tissue
P3
K562
COS
NIH 3T3
PC12
CHO
Mouse Blood (4% EDTA)
Mouse Liver
Mouse Tail
Escherichia coli
Staphylococcus epidermis
Saccharomyces cerevisiae
Human Blood (EDTA)
Human Blood (Heparin)
Human Blood (Citrate)
Human Blood (EDTA) : 3MNOPQ
Human Blood (EDTA Tubes)
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3 x 10 cells
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1.5 x 10 cells
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2.25 x 10 cells
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8.25 x 10 cells
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1.9 x 10 cells
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300µl
300 µl
300 µl
3 ml
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40 mg
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10
9.5-12.5
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15-20
10-30
20
6-13
4.5-6.5
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7
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Wizard SV Genomic DNA Purification System
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A2360
50 preps
A2361
250 preps
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Wizard® SV 96 Genomic DNA Purification System
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A2371
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Cross-contamination assay. Genomic DNA purified from mouse tail clipping
samples or water samples arrayed in a 96-well plate. No PCR product was
expected from water samples.
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Beckman Coulter
Eppendorf epMotion
Tecan
Caliper Life Sciences
PerkinElmer
Yield(AÉ) and purity (BÉ) measurements from 96 separate 20mg mouse tail
clippings using the WizardR SV 96 Genomic DNA Purification System.
P5
96-well genomic DNA purification «ÌÉ。
× Biomek® 2000
Biomek® 3000
Biomek® FX
Biomek® NX
Eppendorf epMotion® 5075 LH
Eppendorf epMotion® 5075 VAC
Tecan Freedom EVO®
Tecan Freedom EVO® with TeMo™
Tecan Genesis® RSP
Tecan MiniPrepTecan TeMo™
SciClone ALH 3000™
PerkinElmer MultiPROBE® II HT/EX
with Gripper
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Genomic DNA
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Wizard Magnetic 96 DNA Plant System
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FF3760
2 x 96 preps
FF3761
4 x 96 preps
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Arabidopsis
Cotton seed
Soybean
Cabbage seed
Grass seed
Squash
Canola leaf
Green pepper seed
Squash seed
Canola seed
Lettuce
Strawberry leaf
Carrot seed
Milkweed leaf
Sunflower seed
Chicory leaf
Potato tuber
Tobacco seedling
Chives
Radish leaf
Tomato leaf
Corn leaf
Rice leaf
Tomato seed
Cotton leaf
Sorghum
Watermelon seed
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Arabidopsis tissue
Canola leaf punches*
Canola seeds
Corn leaf punches*
Cotton seed
Lettuce leaf punches*
Melon seed
Radish leaf punches*
Soybean (10mg)
Squash seed
Sunflower seed
Tomato leaf punches*
% &
10 ng/mg
26 ng/leaf punch
343 ng/seed
98 ng/leaf punch
29 ng/seed
13 ng/leaf punch
166 ng/seed
89 ng/leaf punch
10 ng/mg bean
279 ng/seed
405 ng/seed
111 ng/leaf punch
*Leaf punches 6mm in diameter.
P6
Genomic DNA
² MagneSil® Genomic,
² Fixed Tissue System
² ReliaPrep™ gDNA
² Tissue Miniprep System
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ReliaPrep™ gDNA Tissue Miniprep System
XY
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A2051
100 preps
A2052
250 preps
Analysis of DNA purified from paraffin-embedded, formalin-fixed 10µm thin sections using the
MagneSil Genomic, Fixed Tissue System. (ABI PRISM® 3100 Genetic Analyzer)
P7
Promega
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ReliaPrep® Blood gDNA Miniprep System
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A5081
100 preps
A8052
250 preps
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Blood was aliquotted into 200µl aliquots and frozen
Promega
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Genomic DNA
² ReliaPrep™ Large Volume
² HT gDNA Isolation System
² Maxwell® 16 System
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ReliaPrep™ Large Volume HT gDNA Isolation System A1751
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96×10ml preps
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DNA from
RNA from
Whole Blood
Buffy Coat
Culture Cells
Animal & Plant Tissue
FFPE Tissue
Mouse Tail
Gram (+/-) Bacteria
DNA Virus
Forensic Reference & Casework Sample
Whole Blood
Animal & Plant Tissue
Culture Cells
RNA Virus
His-tagged Protein from
Bacterial Culture
Mammalian Cells
Insect cells
Culture Medium
Reliaprep™ large volume HT gDNA isolation system on the Hamilton microlAb® starplus.
=ReliaPrep™ LV 32 HSM，©ªÍ?>
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Plasmid DNA Promega
、 、 Wizard® Plus
PureYield™
Wizard® SV
Purification Systems
SV Minipreps
Minipreps
96/9600
Wizard® MagneSil® Wizard® MagneSil
Plasmid
Tfx™
Small-Scale Plasmid Systems (<20µg)
Single-Column
PureYield™
PureYield™
Midipreps
Maxipreps
Large-Scale Plasmid Systems
Single-Column
High-Throughput
Plasmid DNA
Plasmid DNA
Specifications
20
20
5
9
20
200
1mg
1-10
0.6-3
1-1.5
0.5-1.5
0.5-1.5
50-250
250-500
Approximate Time
45 minutes
10 minutes
40–60 minutes
45 minutes
45 minutes
45 minutes
75 minutes
Technology
Membrane
Silica
Membrane
Magnetic
Magnetic
Membrane
Membrane
M
M
M/A
A
A
M
M
˜
˜
˜
Yields Up To (µg)
Starting Culture Volume (ml)
Manual/Automated
Applications
˜
Transfection
In vitro Transcription
˜
˜
˜
˜
˜
˜
˜
Cloning
˜
˜
˜
˜
˜
˜
˜
Probe Generation
˜
˜
˜
˜
˜
˜
˜
Sequencing
˜
˜
˜
˜
˜
˜
˜
PCR
˜
˜
˜
˜
˜
˜
˜
Restriction Digestion
˜
˜
˜
˜
˜
˜
˜
Transformation
˜
˜
˜
˜
˜
˜
˜
A1330
A1222
A2250
A1630
A2380
A2492
A2392
Cat No
P10
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transcription/ translation。
TM
PureYieldTM Plasmid PureYieldTM Plasmid PureYield Plasmid
Miniprep System
(µg)
Midiprep System
Maxiprep System
Small-Scale
Large-Scale
Large-Scale
Plasmid System
Plasmid System
Plasmid System
Single-column
Single-column
Single-column
15
200
1000
0.6-3
50-250
250-500
10
30-45
75
PQRS
Mem
Mem
Mem
T/VW
VW
VW
VW
Transfection
●
●
●
In vitro Transfection
●
●
●
Cloning
●
●
●
Probe Generation
●
●
●
Sequence
●
●
●
PCR
●
●
●
Restriction Enzyme
●
●
●
Transformation
●
●
●
MN67=O (ml)
G (min)
Mem＝Silica Membrane；●＝Recommended
P11
² PureYield™ Plasmid Miniprep System
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PureYield™ Plasmid Miniprep System
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A1223
100 preps
A1222
250 preps
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Plasmid DNA
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Transfection of pGL4.13 Vector and assay in 5 different cell lines.
P12
² PureYield™ Plasmid Midiprep System
² PureYield™ Plasmid Maxiprep System
%¿•\，8Ǐ( in vitro)Í
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Expression of firefly luciferase from SP6 Control Plasmid isolated with
PureYield™ Midipreps and competitor midipreps in TNT® SP6 Quick
Coupled Transcription/Translation System.
Kit control is purified via phenol/chloroform and ethanol precipitation.
Plasmid DNA
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PureYield™ Plasmid Midiprep System
！
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A2492
25 preps
A2495
100 preps
A2392
10 preps
1
Kit control
A2393
25 preps
2
PureYield™ Plasmid Midiprep
3
Competitor purified plasmid
Lane Sample
PureYield™ Plasmid Maxiprep System
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Isolated plasmid was transfected into CHO cells and luciferase expression
measured with the ONE-Glo? Luciferase Assay System.
P13
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² Wizard® Plus SV Minipreps
² DNA Purification System
² Wizard® SV 96 Plasmid DNA
² Purification Systems
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A1330
50 preps
A1460
250 preps
A1465
1000 preps
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1x96 preps
Purification System
A2255
5x96 preps
Wizard® SV 9600 Plasmid
A2258 100x96 preps
DNA Purification System
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High-copy pGEM®-3Zf(+) Vector(a) (lanes 1–3) and low-copy pALTER®-1
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enzyme reactions.
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DNA Fragment Promega
DNA Fragment
Wizard®SV
Wizard®
Wizard® DNA
Wizard® SV 96
Wizard® MagneSil®
PurificationSystems
Gel and PCR
PCR Preps
Clean-Up
PCR Clean-Up
Sequencing
Reaction Clean-Up
High-Throughput
Clean-Up
Single-Column
Specifications
DNA Fragment
Yields Up To
95%
96%
80%
90%
Phred 20 out to at
least 650 bases
Approximate Time
15 minutes
15 minutes
15 minutes
20 minutes
35 minutes
Technology
Membrane
Resin
Resin
Membrane
Magnetic
M
M
M
M/A
A
PCR Clean-Up
˜
˜
˜
Gel Extraction
˜
˜
˜
Clean-up from Enzymatic Reactions
˜
Nucleotide Removal
˜
˜
˜
˜
˜
A9281
A7170
A7280
A9341
A1831
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Applications
Cat No
P15
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² Wizard® SV Gel and PCR Clean-Up System
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Wizard® SV Gel and PCR Clean-Up System
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A9281
50 preps
A9282
250 preps
A9285
1000 preps
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55bp
26%
32%
70bp
39%
41%
85bp
55%
61%
100bp
84%
65%
500bp
89%
91%
1,000bp
92%
75%
1,500bp
92%
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3,199bp
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9,416bp
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23,130bp
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Wizard® DNA Clean-Up System
A7280
100 prep
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Wizard® PCR Preps DNA Purification System
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A7170
50 prep
A2180
250 preps
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P17
RNA P18
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SV Total RNA
RNA
Purification Systems
"gDNA#$
PureYield™RNA
SV 96
Maxwell® 16
MagneSil
PolyATtract®
PolyATtract®
Minipreps
Total RNA
Systems
Total RNA
System 1000
mRNA
Total RNA
Single-Column
mRNA
Single
High-Throughput
Specifications
0.27-5.3
Yields Up To (µg)
1mg
0.33-0.45
Varies
2µg /105 cells
5
µg/mg tissue
µg/ 10 cells
70 to20ng/mg
1-2% of
tissue
Total RNA
Approximate Time
60 minutes
90 minutes
30 minutes
30–40 minutes
40 minutes
45 minutes
45 minutes
Technology
Membrane
Membrane
Membrane
Magnetic
Magnetic
Magnetic
Magnetic
M
M
M/A
A
M/A
M
M
Animal Tissues
˜
˜
˜
˜
˜
˜
Cells
˜
˜
˜
˜
˜
˜
Manual/Automated
Sample Type
+
+
Whole Blood
˜
»
˜
Bacteria
˜
˜
»
Yeast
˜
Plant
˜
»
»
˜
˜
Total RNA
Concentration
Cat No
● :,
»:, +:ÊmËty†
˜
˜
Z3100
Z3740
˜
Z3500
AS2000
Z3351
Z5420
Z5200
RNA
RNA Clean-Up and
u RNA P19
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SV Total RNA Isolation System
PureYield™ RNA Midiprep System
MagneSil ® Total RNA mini-Isolation System
Maxwell® 16 System
SV Total RNA Isolation System
PureYield™ RNA Midiprep System
PolyATtract ® System 1000
SV 96 Total RNA Isolation System
MagneSil ® Total RNA mini-Isolation System
Maxwell® 16 System
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SV Total RNA Isolation System
PureYield™ RNA Midiprep System
PolyATtract ® System 1000
SV 96 Total RNA Isolation System
MagneSil ® Total RNA mini-Isolation System
Maxwell® 16 System
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< PureYield™ RNA Midiprep System
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RNA
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SV Total RNA Isolation System
RQ1 RNase-Free DNase I
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Z3101
10 preps
Z3100
50 preps
Z3105
250 preps
M6101
1000 u
RNA yield from mouse liver
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Alteromonas
Bacillus
Chlamydia
Escherichia
Klebsiella
Lactobacillus
Microcystis
Mycobacterium
Neisseria
Orchrobactrum
Photorhabdus
Pseudomonas
Rhizobium
Rhodopseudomonas
Salmonella
Simkania
Streptococcus
Streptomyces
Synechocystis
Vibrio
Yersinia
Saccharomyces cerevisiae
Blumeria grominis
Bierkandera sp.
Gigaspora margarita
Trichophyton rubrum
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Medicago truncatula
Nicotiana tabacum
Pisum sativum
Prunus cerasifera
Trifolium repens
Vigna sp.
Vitis vinifera
※：0800-231-914
45
(mg)
%&
%F
A260/A230 A260/A280
(µg/prep) (µg/mg tissue)
Liver
30
133
4.4
2.4
1.9
Kidney
20
46
2.3
2.1
1.9
Heart
60
16
0.3
1.8
2.1
Spleen
15
79
5.3
2.3
1.9
Brain
60
39
0.7
2.1
2.1
Lung
60
36
0.6
2.0
2.1
30
22
Muscle
RT-PCR amplification
of total RNA isolated
from fresh human
blood
RS&
0.7
1.8
2.1
5 x 10 cells
51
-
2.0
2.0
Tomato Leaf
30
4.6
0.15
1.35
2.00
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S. cerevisiae
7
4x 10 cells
19
-
1.6
2.1
^_
E.coli
9
36.1
-
1.64
2.0
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RAW264.7 Cell Line
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1x 10 cells
RNA
Arabidopsis thaliana
Beta vulgaris
Brassica napus
Cichorium intybus
Hordeum vulgare
Lotus japonica
Lycospersicon esculentum
Manihot exculenta
Genomic DNA detected in 100 ng RNA
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PureYieldTM RNA Midiprep System
XY
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Z3741
50 preps
Z3740
10 preps
Microarray analysis of total RNA
Total RNA isolated from 5 × 107 HeLa cells using the PureYield™ RNA Midiprep System
BCTUVD
-?¶%UÀÝTRIzol Reagent
Sample Amount /
Average Yield
Average
Average
Maximum Sample Capacity
per prep(µg)
A260/A230
A260/A280
Liver
300mg
990.7
1.8
1.9
Lung
300mg
193.9
2.0
2.1
Kidney
200mg
329.0
2.3
2.1
Spleen
150mg
430.9
2.3
2.1
Brain
300mg
305.5
2.3
2.1
Heart
300mg
255.3
2.2
2.1
Muscle
300mg
115.1
2.1
2.1
0
0
1x1010cells
782.7
2.5
2.1
0
0
1.5
2.1
Sample Type
Rat Tissues
Bacteria
E coli
Plant Tissue
Canola
300mg
87.8
Cell Lines
RNA
Total rna was isolated from rat tissues using the Pureyield® RNA Midiprep System.
Lanes 1–2, 150mg spleen
Lanes 3–4, 200mg kidney
Lanes 5–6,300mg liver
Lanes 7–8, 300mg muscle
Lanes 9–10, 300mg heart
Lanes 11–2, 300mg brain
Lanes 13–14,300mg lung
Lanes 15–16, control liver samples.
Lanes M1, Promega Lambda DNA/Ecori + Hind III Markers (Cat.# G1731).
Lanes M2, Promega 100bp DNA Ladder Markers (Cat.# G2101).
0
0
HEK 293T
5x107cells
453.3
2.1
1.9
HeLa
5x107cells
329.2
1.8
2.0
20ml(10ml/tube)
-10*
*
*
Human Blood
*Varies by white cell count. A white cell count of ~5x106 cells/ml yields ~10µg of total RNA.
² PolyATtract® mRNA Isolation System
MmRNA! £¤¥!!
;®HJ，T"Íf¶mRNA!!
P22
¢ ›=，45yz>?ŒmRNA。
¢ ;®°，?Þ99%ßD¶ribosomal RNA(rRNA)。
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XY
Z[
PolyATtract®mRNA Isolation System II with Magnetic Stand*
Z5200
3 isolations
PolyATtract® mRNA Isolation System I*(Refill for Z5200)
Z5210
3 isolations
PolyATtract® mRNA Isolation System III with Mangetic Stand*
Z5300
15 isolations
PolyATtract® mRNA Isolation System IV*(Refill for Z5300)
Z5310
15 isolations
BCTUVD
RNA
Product List
：
：(02)2655-7678 ：0800-231-914
Genomic DNA
Product
Wizard® Genomic DNA Purification Kit
Wizard® SV Genomic DNA Purification System
ReliaPrep™ Blood gDNA Miniprep System
ReliaPrep™ gDNA Tissue Miniprep System
ReliaPrep™ Large Volume HT gDNA Isolation System
Wizard® SV 96 Genomic DNA Purification System
MagneSil® ONE, Fixed Yield Blood Genomic System
MagneSil® Blood Genomic, Max Yield System
MagneSil ® Genomic, Fixed Tissue System
MagneSil® KF, Genomic System
Wizard® Magnetic 96 DNA Plant System
PureYield™ Plasmid Midiprep System
PureYield™ Plasmid Maxiprep System
Wizard ® Plus SV Minipreps DNA Purification System
Wizard ® SV 96 Plasmid DNA Purification Syste
Wizard ® SV 9600 Plasmid DNA Purification System
：(06)209-4380
mRNA
Size
100 isolations × 300µl
500 isolations × 300µl
100 isolations × 100µl
50 preps
250 preps
100 preps
100 preps
250 preps
96 × 10ml
1 × 96 preps
4 × 96 preps
1 × 96 preps
1 × 96 preps
100 samples
200 preps
2 × 96 preps
4 × 96 preps
Cat No
A1120
A1125
A1620
A2360
A2361
A5081
A2051
A2052
A1751
A2370
A2371
MD1370
MD1360
MD1490
MD1460
FF3760
FF3761
Product
PolyATtract® mRNA Isolation System II with with Magnetic Stand
PolyATtract® mRNA Isolation System I (Refill for Z5200)
PolyATtract® mRNA Isolation System III with with Mangetic Stand
PolyATtract® mRNA Isolation System IV (Refill for Z5300)
PolyATtract® System 1000 with Magnetic Stand
PolyATtract® System 1000 without Magnetic Stand
Size
100 preps
250 preps
25 preps
100 preps
10 preps
25 preps
50 preps
250 preps
1 x 96 preps
5 x 96 preps
100 x 96 preps
Cat No
A1223
A1222
A2492
A2495
A2392
A2393
A1330
A1460
A2250
A2255
A2258
Size
3 isolations
3 isolations
15 isolations
15 isolations
3 isolations
3 isolations
Cat No
Z5200
Z5210
Z5300
Z5310
Z5420
Z5400
Total RNA
Product
SV Total RNA Isolation System
PureYield™ RNA Midiprep System
SV 96 Total RNA Isolation System
MagneSil® Total RNA mini-Isolation System
MagaZorb® Total RNA Mini-Prep Kit
Plasmid DNA
Product
PureYield™ Plasmid Miniprep System
：(04)2263-3813 Size
10 preps
50 preps
250 preps
10 preps
50 preps
1 x 96 each
5 x 96 each
4 plates
200 preps
Cat No
Z3101
Z3100
Z3105
Z3740
Z3741
Z3500
Z3505
Z3351
MB2004
Size
50 preps
250 preps
100 preps
1 × 96 preps
4 × 96 preps
8 × 96 preps
50 preps
250 preps
Cat No
A9281
A9282
A7280
A9340
A9341
A9342
A7170
A2180
DNA Fragment
Product
Wizard® SV Gel and PCR Clean-Up System
Wizard® DNA Clean-Up System
Wizard® SV 96 PCR Clean-Up System
Wizard ® PCR Preps DNA Purification System