Bio 205 & Bio 298AA– Spring 2016
Laboratory Policies and Schedule
Mrs. Anne Healy
Office phone: 480-423-6779
E-mail: anne.cedergren-healy@scottsdalecc.edu
Office: NS 118
Office hours:
MTWTh
See me in lab after lab is complete.
Fri
10:30 a.m. – Noon in my office, NS 118
Grading:
Lab quizzes (6 quizzes @ 10 points each)
(8 quizzes taken, lowest 2 scores dropped)
Lab Midterm
Lab Final
Total Lab Points
60
100
100
----------260
Safety Procedures:
You must wear closed-toe shoes (no sandals), goggles, lab coat, and hair
clips/ponytail holders for long hair at each and every lab.
NO EATING, DRINKING, CHEWING GUM, OR APPLYING MAKE-UP IN
LAB!
If you know or suspect that you may be at increased risk of infection due
to pregnancy, autoimmune disease, cancer, recent surgery or positive HIV
status, it is your responsibility to inform your health care provider that you are
taking microbiology lab.
Lab work: During each lab exercise, I will instruct you to record some data,
answer questions, or make drawings of specimens. Lab work is due the
following week. Since labs cannot be made up, your lab grade will drop by at
least 5 points for each lab you miss.
Lab Quizzes: There is a lab quiz most weeks (with the exception of the 1st
week). Missed lab quizzes may not be made up, but your 2 lowest lab quiz
grades will be dropped. Missed lab work and lab exercises may not be made up.
Lab drawers: There are plenty of drawers that you may use to store your
lab coat, goggles, old sneakers, or hair clips between lab periods.
Week of:
Lab exercises
1/25
Overview of lab rules
Introduction to the light microscope
Effectiveness of hand washing
2/1
Lab quiz 1
Common Aseptic Transfers and Inoculation Methods
Ubiquity of Microorganisms
Simple Stains
2/8
Lab quiz 2
Gram Stain
Acid-fast Stain
2/15
No labs this week due to Presidents’ Day holiday.
2/22
Lab quiz 3
Capsule Stain
Endospore Stain
2/29
Lab quiz 4
Handout: PCR
Handout: Yeast ID using MUGAL and PRO
3/7
Lab Midterm Exam
Finish PCR using Gel Electrophoresis
3/14
SPRING BREAK
3/21
Phenylethyl alcohol agar
Mannitol salt agar
Snyder agar
Catalase test (inoculate Nutrient agar)
3/28
Lab quiz 5
StaphAurex Test using growth from last week’s MSA plate.
PYR test using E. faecalis.
Catalase test follow-up
Inoculate:
OF media
MRVP broths
Citrate slants
Oxidase test
EMB agar
4/4
Lab quiz 6
Follow-up tests and record results on:
MRVP broths
Oxidase test on BBL Dry Slide
4/11
Lab quiz 7
Anaerobic jar
Blood & Chocolate agars
Bacterial transformation
4/18
Lab quiz 8
Antiseptics & disinfectants
Antibiotics testing (Kirby-Bauer)
Germicidal effect of UV light
4/25
ELISA
5/2
Lab Final Exam
Microbiology Lab Rules
These apply to all students taking Bio 205 or Bio 298AA.
Failure to observe rules will result in disciplinary action.
1. Do NOT eat, drink, chew gum, or apply lip balm or cosmetics – EVER!
2. Store all nonessentials (books, purses, phones) on the shelves or in the drawers.
3. Wipe down lab work area with Amphyl disinfectant.
4. Wash hands with Hibiclens.
5. Do NOT remove microbes or lab equipment from lab.
6. Wear goggles, closed-toe shoes, lab coat (or old clothing), gloves, and tie back
long hair.
7. If you are feeling too sick to come to lab, leave me an email or voice mail
message or tell me after lecture.
8. If you are pregnant, immunocompromised, scheduled for surgery, or taking
immunosuppressive drugs, please inform me privately. It may be in your longterm best interest to take micro another semester and I may ask for a note from
your doctor authorizing you to take micro.
9. Cover scratches on hands with a bandage (we have some) and then a glove.
10. Never pipette by mouth.
11. Dispose of used slides and broken glass in the white plastic “sharps” box that I
will point out.
12. If you spill something, please notify me immediately. Same if a part falls off your
microscope.
13. Keep test tubes upright in racks. Keep cultures covered if you are not using them.
14. Contaminated test tubes: remove tape and place in racks on discard counter.
Contaminated Petri dishes, swabs, or gloves: toss in autoclave bag.
Lab Syllabus Acknowledgement
I am aware that there are risks involved in performing microbiology
lab procedures and that I will be working with potentially hazardous
chemicals and living microorganisms. If I suspect I am at increased risk of
infection, I agree to notify my health care provider that I am taking
microbiology lab and my instructor so accommodations can be made, if
possible. I have read the lab safety information and rules and comprehend
their importance. I will employ proper techniques at all times while working
in the lab in order to minimize the dangers. If a procedure is unclear, I will
ask my instructor for clarification.
Student name (print): _________________________________
Student signature: ____________________________________
Date: _____________________