MICR 22 – Microbiology (WINTER 2014)
Instructor: Dr. Tim Revell
Office 60-2102, Ext# 4231 email: trevell@mtsac.edu
Office Hours: By appointment only
PLEASE READ THIS ENTIRE SYLLABUS
WebPage: http://instruction.mtsac.edu/trevell
Text Book: Tortora, Funke, and Case, 2012. Microbiology, An Introduction. 11th ed
Lab Manual: Anderson, C; Great Adventurers in the Microbiology Laboratory (6th)
Optional: Control of Communicable Diseases by Heymann, David 2009
Optional: A Photographic Atlas for the Microbiology Laboratory
Optional: A good medical dictionary, such as Tabers.
Other good sources: www.cdc.gov and www.microbiologyplace.com
LECTURE SCHEDULE:
Order :
Lecture Topic
Chapters to Read
1
2
3
4
5
6
7
8
9
10
11
12
12
11
12
13
14
15
16
17
18
19
20
21
Microbes and You
Principles of Disease and Epidemiology
Observing Microbes through a microscope
Functional Anatomy of Prokaryotic/Eukaryotic cells
Classification of Microbes
The Prokaryotes: Domains Bacteria and Archaea
Exam I
Eukaryotes: Fungi, Algae, Protozoa, and Platyhelminthes
Viruses, Viroids, and Prions
Microbial Metabolism
Microbial Growth
Exam II
The Control of Microbial Growth
Antimicrobial Drugs
Microbial Genetics
Biotechnology and Recombinant DNA
Exam III
Microbial Mechanisms of Pathogenecity
Innate Immunity: Nonspecific Defenses of the Host
Adaptive Immunity: Specific Defenses of the Host
Highlights from: Practical Applications of Immunology
Highlights of Disorders Associated with Immunology
Exam IV
FINAL
(Feb 13th)
1
14
3
4
10
11
12
13
5
6
7
20
8
9
15
16
17
18
19
Prerequisite: CHEM 10 and CHEM 40
Advisory prerequisite: BIO 1 and BIO 4 or BIO 4H
Points Possible
Lab Quizzes (9 @ 20 points)
Unknown
Challenge
Lab Exercise (19 @ 3 points)
The Pathogen Test
Lecture Quiz (4 @ 10 points)
Lecture Exams (4 @ 100 points)
Lecture Final
Letter Grade and Percentages
180
20
15
57
60
40
400
250
1022*
100-90 % A
89-80% B
70-79% C
60-69% D
< 60% F
* This total is approximate and subject to change based on holidays and other events.
IMPORTANT DATES:
Jan 20thth – Martin Luther King Jr. Day
Assignments:
Quizzes/Exams: Usually given in the beginning of class. No make-ups on any quiz or
exam or test. Quizzes and exams will consist of fill-in, multiple choice, matching, truefalse, and short essay.
The Pathogen Test: This is done during lab (see schedule) and will cover all of the
pathogens we covered throughout the semester.
Lab Exercise: These are done in class and will be turned in for credit. YOU CANNOT
turn in a lab if you were not in class the day the lab was done. If you do so, this will count
as plagiarism and you will receive a zero on the assignment and possibly an “F” in the
course. Lab work is done in groups, HOWEVER, each write-up should be done
individually AND in your own words.
Materials you will need:
Turn into the instructor:
6 Scantron 815’s (Do NOT write anything on the scantrons…just bring them!)
4 Scantron 886’s
1 Scantron 884’s
11 Scantron 882’s
One Sleeve of 20 Petri Dishes (DO NOT OPEN)
You will keep and hold on to:
Lab coat (third day of class)
Box of gloves
Eye protection and closed toed shoes
One booklet of 4X6 inch lens paper
Other information you need to know:
1) Attendance: Students are expected to attend all classes and labs and are expected to
stay for the entire length of the class without exceptions. If you leave early, you must
inform the professor and you lose points as mentioned above.
2) Check your Mt. SAC email OFTEN!!!! I will often send important information
out via the Mt. SAC email system. I will not send email to any other account!
Please check your Mt. SAC email everyday.
3) The attached lecture/lab schedule is intended to assist you in planning your readings for
this course. I will expect you to have read the appropriate text sections prior to class. I
will make every effort to maintain this schedule. Modifications, however, may be
necessary. It is your responsibility to attend all classes and be aware of any changes
that I may announce.
4) All assignments are due at the beginning of class. Late work (even if it is 5 minutes late
is worth ½ credit). You have two days to complete late assignments (other than extracredit which will receive a zero if it is late). After that, no credit will be given.
5) Note: It is the student’s responsibility to drop a course officially if the student wishes
to avoid an “F”. The instructor MAY drop students for non-attendance, but this is
discretionary. Students who do not drop but do not complete the work will receive an
“F”.
6) Academic Honesty: Mt San Antonio College regulations and guidelines regarding
academic honesty will be followed and enforced. Cheating or plagiarism may result in
an F for the entire course. I will also write a letter stating that you have cheated and it
will become part of permanent Mt. SAC record. Do not cheat or plagiarize in any
form! The possession of any cheating items (ie, notecards, cell phones, ect.) during an
exam will be interpreted as a cheating incident regardless of whether or not you
actually “used” the item(s). All students must sign and return the Mt. SAC Biology
department cheating policy. All cheating incidents will be treated the same; it does not
matter whether it was a quiz, exam or assignment.
7) Attitude and success: This is a college level biology course and it is a difficult course.
The study of biology, even at a general level, is very complex. I teach this class at a
college level and I expect that you will study and prepare at a college level. I will not
lower my standards just to make the class easy for you. If you have the attitude that
this class (or the professor) is “not fair” or its “too hard” then your negative attitude
will probably prevent your from passing my class. I also believe, if you always work
hard, show up every day, come to my office hours if you need help, etc. that you will
pass my class. Remember, if nobody is passing the class, then it is probably my fault.
But if YOU are not passing (but a bunch of other people are) then it is probably NOT
my fault.
8) The instructor reserves the right to make any necessary changes to this syllabus or to
any part of the class without prior warning.
9) To succeed in this course: Your study of biology will be an exciting and rewarding
experience. We will cover a variety of topics that can be both interesting and useful in
improving your life and helping you achieve your career goals. However, do not make
the mistake of taking your study of this course too lightly. To succeed in this course
you MUST attend class regularly, study often AND effectively, and live a life that is
conducive to learning (for example, you need to sleep, eat, work, etc. within your
means!). Failure to meet these basic requirements will make success difficult for most.
10) Grades on Quizzes, Exams, etc. - Students will be given all assignments and exams
back in a timely manner. If there is a grading error of any sort, the student has two
days to notify the instructor and solve the problem from the date in which the
exam/quiz/assignment was handed back to the class. No reconsideration of any grade is
possible without evidence, and it is the student’s responsibility to demonstrate the basis
of any grade change. If you do not meet with the instructor within that two day period,
the original grade is final. The two day period begins when I hand the
exam/quiz/assignment back to the class; not necessarily when you receive it (i.e., if you
are absent and do not pick up the exam for three weeks, you have missed you
opportunity to negotiate your score. Students should keep track of their grade on
the sheet provided.
11) I keep ALL exam and quiz questions. YOU MAY NOT, under any circumstances,
take one of my exams or quizzes home with you. YOU MAY NOT zerox, copy, or
photograph any of my exams or quizzes. Failure to follow these rules may result in an
"F" in the course.
12) Incomplete policy: It is the students responsibility to drop the class if that is their
intention. An incomplete may be given if all three of the following conditions are met
1) The student has completed ALL course material (other than the final exam) 2) the
student is passing with at least a “C” 3) the student can demonstrate they are unable to
complete the remained of the semester. The instructor reserves the right to accept or
reject a students request for an incomplete.
13) Again, Biology is an exciting but challenging class. DO NOT just memorize your
notes. I expect you to UNDERSTAND biology. To understand biology you need to
ask questions, think, and analyze the material I give you. Forming study groups is
often a beneficial method of attack, but only if your groups is serious about studying!
14) No cell phones or any other devices that create distractions are allowed in class.
Special considerations may be given by the instructor on an individual basis. Any cell
phone that is used, looked at, or even visible by the instructor during a quiz or exam
will result in a zero on that assignment and a possible “F” in the entire course. Not
texting is allowed in class (even during breaks!). No texting in lecture or lab at ANY
TIME. Even during breaks! Go outside if you need to use an electronic device.
15) Please notify the instructor immediately if you have a particular situation that
requires special accommodations (such as a health issue or disability).
16) SLO’s and GEO’s related to this course can be found on the Mt. SAC website at
www.mtsac.edu
17) We do use several chemicals and organisms in this lab that are known to potentially be
hazardous to your health. Please discuss any health related questions you have with
your physician if you are concerned.
Lab Regulations:
1) No Food or Drink Allowed in any lab at any time.
2) Wash your hands often and upon entering/leaving lab (happy birthday twice!)
3) MUST wear a lab coat
4) MUST wear closed toed shoes
5) Never apply cosmetics or put in/take out contact lenses
6) Clean lab table BEFORE and AFTER with germicidal solution
7) Keep your desk and floor free of non-essential materials at all times.
8) Know where the fire extinguishers are and eye wash, and shower are located.
9) Keep cultures in test tube racks to prevent accidents
10) Hot test tubes should be handled with test tube holders
11) Never remove media, equipment, or bacterial cultures from the lab.
12) No visitors allowed in the laboratory at any time.
13) Immediately cover spilled cultures or broken cultures with paper towels and then
saturate with germicide (for 15 minutes!). Broken glass goes into the “glass box” (NOT
the trash).
14) Cover slips and broken glass goes into the “glass box”
15) Immediately report any cuts or burns to the instructor.
16) When handling contaminated materials:
a) Do not put contaminated instruments (such as inoculating loops) on bench tops!
b) NEVER pipette by mouth!
c) contaminated plates, swabs and disposable pipettes go into the biohazard box.
d) Contaminated test tubes must be placed in the wire baskets provided in the
autoclave bin in the back of the room.
e) Live mounts of hazardous organisms must be soaked in germicide for 15 minutes
before washing with soap and water.
17) When using the Bacti-cinerators:
a) The reach optimum sterilization temperature after 10 minutes. Please turn off
the bacti-cinerator at the end of the lab but leave them plugged in.
b) Do not let your inoculating loop or needles stay inside the bacti-cinerator
unattended!
18) Labeling:
a) Only use wax pencils to write on class test tubes
b) petri plates your prepare can be labeled with your permanent marker.
c) ALL petri plates, test tubes, and slides must be properly labeled and include,
your name, date, lab time and the specimen(s) used for inoculation.
19) We do use several chemicals and organisms in this lab that are known to potentially be
hazardous to your health. Please discuss any health related questions you have with your
physician if you are concerned.
I have read and agree to follow the above rules and regulations:
Print Your Name
Sign your name:
Date:
_____________________
__________________________
___________
Mt. San Antonio College - Microbiology 22 Lab Schedule for Winter 2014
Week Date
1
Lab Activities:
Jan. 6
Date
Jan. 7
Orientation with
Introductions &
Safety Rules/Regulations &
Pathogen Groups
1
Jan. 8
Exercise #1: The Microscope
Jan. 9
Exercise #1 continued:
Quiz 1 (lab safety/regulations
& Lab Exercise 1)
The Microscope and
Introduction to Bacteria!
2
Lab Activities:
Jan. 13
Exercise #2:
Culturing the Environment and
Your Hands!
Jan. 14
Exercise #3:
Media, Aseptic Techniques
and Handling Cultures
Quiz 2 (Lab Exercises 2 & 3 +
PG1)
(+ Discuss PG1)*
Exercise #4:
Simple Staining
2
Jan. 15
Jan. 16
Exercise #5:
Exercise #5 continued:
Gram Stain
Acid-fast and Spore Stain
(PG 2)*
3
Jan. 20
Jan. 21
Martin Luther King, Jr.
Holiday!
Quiz 3 (Lab exercises 4 & 5 + PG
2)
Unknown staining
3
Jan. 22
Unknown Staining to
completion (first hour)
Plus…..
Exercise #6:
Fungi Lab observations
(PG 3)*
Jan. 23
Quiz 4 (fungi lab+ PG3)
Exercise #7:
Protozoa Lab observations
AND
Exercise #8:
Worms/Arthropods Lab
observations (PG 4)*
4
Jan. 27
Jan. 28
Quiz 5 (protozoa lab+ PG4)
Quiz 6 (worms/arthropods lab+
PG5)
Perform Exercise #9: Pure
Culture Techniques
1. Record results from previous
lab
_______________________
2. Perform Exercise #11: More
Lab Tests (of the isolated
“unknown bacterial UTI
specimen”)
And
Exercise #10: Perform
“isolation of unknown
bacterial pathogens” on
specialized media
(complete A, B & C)
3. And the API 20 E System
(Exercise #12) is put to use for
helping ID our “bacterial
pathogens”
(+ Discuss PG 5)*
(+ Discuss PG 6)*
4
Jan. 29
Jan. 30
Subculture for pure culture
challenge points
Quiz 8 (Lab Exercises 10, 11, 12
and 13 + PG 7)
Quiz 7 (Exercise #9 + PG 6)
Record results of your
UTI’s lab tests and
Record API 20E results
and key out your
“Unknown pathogen”
Exercise #13: Confirmation
of the UTI results!
Discussion time, as needed.
(+ Discuss PG 7)*
Start ‘Control of Microbes’ with
3 Labs
1. 1.
Exercise #14:
Antimicrobial
Susceptibility Testing.
5
Feb. 3
Record results of the entire
S, I, and R ratings and
discuss.
Feb. 4
AND…..
Record & discuss results of
Exercise #15
__________________________
3. Exercise #16:
Perform Antibacterial Products
Testing (each student brings a
favorite antiseptic or
disinfectant to test ).
2. Exercise #15: Perform
Heat, Cold, Drying,
Radiant Energy Lab
(+ Discuss PG 8)*
5
Feb. 5
Feb. 6
Exercise #17: Simulation of
Disease Spread: an HIV
epidemic + HIV antibody
detection with enzyme linked
immunosorbent (ELISA) assay!
Record results of
antibacterial products
tests.
+ Quiz 9 (Lab Exercises 14,
15 & 16 + PG8)
6
Feb. 10
(+ Discuss PG 9)*
Feb. 11
Record Transformation results
& discuss
_______________________
Exercise #19: Perform
Milk/Water lab (each student
brings)
Exercise #18:
Transformation of E. coli
experiment
6
Feb. 12
Pathogen Test in Lab!
Feb. 13
Record milk/water results
Lab Quiz 10 ( Lab Exercises 17,
18 & 19 + PG 9 )
FINAL EXAM in lecture
* Date that pathogen group (PG#) should be completed so you’ll be ready for the next quiz! Bring your charts for
discussion/sharing.