2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
Traders Hotel, Manila
February 21-22, 2013
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
TABLE OF CONTENTS
Message from the DOST Secretary
Message from the DOST Undersecretary for R&D
Message from the SEI Director
Message from the ASTHRDP-NSC Steering Council Chairman
About ASTHRDP and the NSC
In Retrospect: ASTHRDP in 2012
Oral and Poster Presentation Sessions
Plenary Speakers’ Profile
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ABSTRACTS OF TECHNICAL ORAL PRESENTATIONS
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Quorum Sensing Inhibition By Non-Polar Fractions from Halymenia Durvillaei Bory De Saint-Vincent From Bolinao,
Pangasinan, Philippines, Espita, Dang Marviluz L./Montaño, Marco Nemesio, UPD
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Isolation, Characterization, and Molecular Cloning of A Novel Serine Protease-Like Gene of the Elastase Sub-Class
from Moringa Oleifera Seed Embryos., Maini/ZA/Panes/VP/Canlas/MKC/RN, Garcia/VS, Tolentino, ADMU
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Synthesis, Morphological, and Structural Characterization of Moistureabsorber Para-Toluenesulfonic Acid-Doped
Polyaniline, Bendoy, Anelyn P./Uy, Mylene M./Vequizo, Reynaldo M., MSU-IIT
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Isolation of High Molecular Weight Community DNA From Rumen Digesta for Metagenomic Studies, Sabinay,
Stpehen G./Laurena, Antonio C./Sevilla, Cesar C./Yebron, Medino Gedeun N. Jr., UPLB
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Cyclic Diarylheptanoid Garugamblin-I Isolated From the Bark of Garuga Floribunda (“Bogo”), Pautong, Putlih Adzra
T./Uy, Mylene M., MSU-IIT
Removal of Organic and Inorganic Pollutants From Aqueous System By Chitosan Molecularly Imprinted Polymer in
Modified and Unmodified Form, Templonuevo, Xyza Jane/Chakraborty, Soma, ADMU
Functional Elucidation of Three Contigs of Marsupenaeus Japonicus to White Spot Syndrome Virus (WSSV) By dsRNA
Inteference, Tare, Maria Violeta/Maningas, Mary Beth/Shitara, Aiko/Kondo, Hidehiro/Hirono, Ikuo, UST
Taxonomy and New Distributions of invasive Suckermouth Armored Catfishes (Pisces: Pterygoplichthys), Chavez,
Joel, DLSU
Identification of Mangroves, Seagrasses and Seaweeds of Nogas Island, Anini-Y, Antique, Morillo-Manalo, Laureen/
Torrechilia-Guzman, Armi May, UPV
Diversity of Some Endemic Nepenthes Species of the Philippines Based on Morphology, rbc L, mat K, and 18S rDNA
Sequences, Acil, Rachel Y./Diaz, Ma. Genaleen Q./Amoroso, Victor B./Laude, Rita P./Mendioro, Merlyn S./
Laurena, Antonio C., UPLB
Immunomodulatory and Chemopreventive Properties of Sulfated Polysaccharides from Sargassum Siliquosum J. G.
Agardh, Vasquez, Ross D./Ramos, John Donnie, UST
Gene Expressions of Pituitary Adenylate Cyclase Activating Polypeptide (tPACAP38)and Vitellogenin (Vtg) in
Tilapia Exposed to De-71 and 17β- Estradiol Under Freshwater and Saline Water Conditions, Calma, Rosemarie R./
Heralde, Francisco M. III/Collazo, Margarita C./Schlenk, Daniel, DLSU
Mcshane and Henstock Equi-integrability of Functions Taking Values in Locally Convex Topological Vector Space,
Acapulco, Juniven P./Benitez, Julius V., MSU-IIT
A Spectrally Negative Lévy Risk Process with Piecewise Constant Tax Structure, Veras, Kathleen Zoe E./Sy, Polly
W./Valdez, Adrian Roy, UPD
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Construction and Structural Stability Analysis of Models of Dopamine D1 Receptor Trafficking in Renal Proximal
Tubule Cells, Villar, John Justine S./Arceo, Carlene Perpetua P./Mendoza, Eduardo R., UPD
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S-Orbit Colourings of Square and Hexagonal, Raymundo, Gregorio Abaya III/Baraya-Walo, Ma. Lailani, UPD
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In the Complement of A Minimum Dominating Set in the Join, Corona and Composition of Graphs, Kiunisala,
Edward M./Jamil, Ferdinand, MSU-IIT
Dually-Weighted Stirling-Type Sequences, Gonzales, Ken M./Manuel Joseph C. Loquias/Corcino, Roberto B.,
UPD
GIS-Based Assessment of Groundwater Vulnerability to Contamination in Boracay Island Using Drastic Model,
Linan, Efren L./Ella, Victor B./Florece, Leonardo L., UPLB
Zooplankton Composition and Abundance in Lamon Bay, Northeast Luzon, Philippines, Bollozos, Iris/Yniguez,
Aletta T./Palermo, Joseph Dominic/Camoying, Marianne/Lopez, Mary Rose/Villanoy, Cesar, UPD
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
TABLE OF CONTENTS
Response of the Juvenile Sea Cucumber Holothuria Scabra To Salinity Stress, Gorospe, Jay R C./Juinio-Meñez, Marie
Antonette, UP-Diliman
Growth Performance and Nutritional Efficiency of Silkworm (Bombyx Mori L.) Strains in Sericulture Center,
University of the Philippines Los Baños, Laguna, Medillo, Japhia V./Ocampo, Virginia R., UPLB
Mapping the Salinity Tolerance QTL of Hasawi At Seedling Stage Using F6 Recombinant inbred Lines Population,
Pascual, Evangeline D./Dela Viña, Celia B./Gregorio, Glenn B./Sajise, Andy/Amas, Junrey/Arceta, Marydee/
Barretto, Aniceta/Vispo, Aiza Naireen, UPLB
Characteristics and Origin of Soils Along the Central Mountain Range in Leyte, Maranguit, Deejay S./Asio, Victor
B., VSU
Electrically Conducting Nis-Montmorillonite Nanocomposite Prepared By Ultrasonic Intercallation and Solid-Solid
Reaction, Bermudez, Stephanie Ann O./Obiles, Romelyn Jane V./Cabañero, Joel M. Jr./Somintac, Armando B. ,
UP-Diliman
Graphene / PEDOT-PSS Composites as Supercapacitor Electrode Materials, Chua, Stephanie L./Binag, Christina
A., UST
Incorporating Graphene in Anthocyanin Dye Sensitizer for Dye-Sensitized Solar Cells, San Esteban, Anna Carissa
M./Enriquez, Erwin P., ADMU
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Fabrication and Characterization of Poly(Hydroxylauric Acid- e-Caprolactone) –Clay Nanofiber Composites: A New
Potential Scaffold for Tissue Engineering, Monserate, Juvy J./Sumera, Florentino C., UP-Diliman
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Functionalization of Glass Substrates for DNA Sensing, Calupitan, Jan Patrick/Enriquez, Erwin P./Rojas, Nina
Rosario L., ADMU
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Charge Pattern Writing on ZnO Microribbon Using AFM Electric Lithography, Permejo, Michael Francis P./
Singidas, Bess G./Vargas, Ray M./Sayson, Luce Vida/Sarmago, Roland V., UP-Diliman
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Ion Currents of Cu- Ion Beams in a Plasma Sputter Type Negative Ion Source and Its Correlation With Sputtering
and Negative Ion Formation, Mahinay, Christian Lorenz S./Malapit, Giovanni M./Ulano, April M./Poral,
Matthew D./Mascariñas, Venice F./Ramos, Henry J., UP-Diliman
Complexity in Human Online Communication: Analysis and Modeling of Sending Patterns in e-Mail and Twitter,
Solatorio, Aivin V./Lim, May T., UP-Diliman
Characterization of Silica-Modified Polyaniline and Zinc Oxide Nanocomposites for Solar Cell Application, Rico,
Christine Adelle L./Alguni, Arnold C./Vequizo, Reynaldo M., MSU-IIT
Experimental Investigation of the Dynamics of a Granular, Hamoy, Efren/Confesor, Mark Nolan, MSU-IIT
Isolation and Identification of Seaweed-Associated Carrageenase-Producing Bacteria, Tremedal, Paul Andrew/
David, Anne Marjorie E./Lluisma, Arturo O., UP-Diliman
Degradation of Hydrocarbon By Halophilic Bacteria Isolated From Two Local Salterns in Luzon, Nanca, Carolyn L./
Dedeles, Gina Rio, UST
An in Silico Analysis of the Effect of Drug Resistance On Virulence Genes of Entamoeba Histolytica, Palma, Juvy Ann
C./Penuliar, Gil M., UP-Diliman
Occurrence and Antimicrobial Activities of Fungal Endophytes From Philippine Mangroves, Moron, Llewelyn S./
dela Cruz, Thomas Edison E., UST
Biodegradation of Untreated, Heat and UV-Treated Polyethylene and Oxo-Biodegradable Plastics By Bacteria
Isolated From Soil, Vargas, Ma Christina B./Chan, Merab A., ADMU
Safety And Bioefficacy Evaluation Of The Essential Oil From Pogostemon Cablin (Blanco) Benth. (Lamiaceae)
Against Aedes Aegypti, Dacanay, Aleth Therese L./Osi, Marina O., UST
Platelet – increasing Property of Euphorbia Hirta Linn. (Euphorbiaceae) in Thrombocytopenic Rat Models, Apostol,
Jovencio G./Santiago, Librado A./Ysrael, Mafel Y., UST
Cutaneous Bacteria of Frogs Collected From Mt Palay Palay Mataas Na Gulod National Park, Cavite for AntiChytridiomycosis Activity, Eda, Rae Abigail R./Diesmos, Arvin C./Chan, Merab A., ADMU
Immunomodulatory and Anti-Scabies Properties of Tinospora Rumphii Boerl Lotion in Sarcoptes Scabiei Var Hominis
-infected Patients and Its Predicted Shelf-Life : A Pilot Study, Castillo, Agnes L./Osi, Marina O./Ramos, John
Donnie A./De Francia, Jean L./Dyjunco, Marylaine U./Quilala, Peter F., UST
Antioxidant and Chemopreventive Properties of Sargassum Siliquosum and its Preformulation, Corpuz, Mary
Jho-Anne T./Osi, Marina O./Santiago, Librado A., UST
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TABLE OF CONTENTS
ABSTRACTS OF TECHNICAL POSTER PRESENTATIONS
3D Reconstruction of Superhydrophobic Surfaces, Buendia, Johanna Evi P./Balili, Ryan B./Candidato, Rolando T.,
MSU-IIT
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A Biodiversity Report On the Myxomycete Assemblages in Leaf Litter, Twigs, Dungs, and Inflorescences, Buaya,
Anthony T./dela Cruz, Thomas Edison E., UST
Acceptability in Goats and Rumen in Situ Degradability of Foliage With Varying pH Levels From Selected Trees and
Shrubs, Aban, Maita L./Bestil, Lolito C., VSU
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Analysis of Ethynylestradiol and Levonorgestrel in River Sludge, Enerio, Pio Gerard A./Dayrit, Fabian M., ADMU
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Analytical Calculation of the Radius of Gyration of a Polymer In Fractional Brownian Motion, Bantayan, Sim P./
Cabahug, Cresente O./Baybayon, Roel N./Arogancia, Dennis C./Gemao, Beverly V./Bornales, Jinky B ., MSU-IIT
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Biological Control of Pectinolytic, Fruit Soft Rot Agents Using Phylloplane Bacteria, Mateo, Bea C./Dedeles, Gina
R., UST
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Biosorption On Cadmium (II) By Yeasts From Fruit Peels, Casamorin, Jovy Ann M./Dedeles, Gina R., UST
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Characteristics and Nutrient Status of Highly Weathered Soils in Silago, Southern Leyte, Piamonte, Maria Katrina
B./Asio, Victor B., VSU
Characterization of the Role of Fluctuations in Predator-Prey Dynamics Driven By Demographic Stochasticity,
Palenzuela, Karlo/Juanico, Dranreb Earl, ADMU
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Decadal and Genre Comparison of Musical Networks From Midi Files, Valdez, Nicole/Cabatbat, Josephine Jill/
Roxas-Villanueva, Marianne/Tapang, Giovanni, UPD
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Describing Compartmentalization in the fore-Wing of Populations of the Rice Grasshoppers, Oxya sp. (Acrididae:
Orthoptera), Coronel, Kimverly Hazel I./Torres, Mark Anthony J./Demayo, Cesar G., MSU-IIT
Design of Electrostatic Ion Accelerator and Focusing System for the Plasma Sputter-Type Negative Ion Source
(PSTNIS), Poral, Matthew D./Ramos, Henry V., UPD
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DNA Barcoding of the Philippine Traditional and Modern Rice Varieties, Uy, Mary Rose DL/Abella, Evaristo A./
Tabanao, Dindo Agustin A., CLSU
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Duadic Codes Over
, Florida, Veronica B./Dicuangco-Valdez, Lilibeth B./Betty, Rowena Alma L., UPD
Effects of Cultured Yeast Supplementation On Growing Kids Fed With Napier Grass-Concentrate Ration, Abela,
Mark V./Bestil, Lolito C., VSU
Effects of Substrate Orientation on the Growth of Zinc Sulfide Nanostructures, Odarve, Hananish Joy G./Alguno,
Arnold C./Vequizo, Reynaldo M., MSU-IIT
Efficiency of the Estimation of the Hurst Exponent in a Time Series Based on Frequency Domain Bootstrapping,
Madronero, Jonathan/Evardone, Chita, MSU-IIT
Ethnobotany of Some Pili (Canarium Ovatum) Varities Native To Gubat, Sorsogon, Chavez, Judith R./Cadiz, Nina
M., UPLB
Fabrication of Aluminum Nitride/Air-Gap Distributed Bragg Reflectors From Wet Etching of Sacrificial Aluminum
Layers, Abat, Jonathan Ray R./Mangila, Nemesio S. IV, Salvador, Arnel A./Somintac, Armando S. , UPD
Floristic Diversity and Avifaunal Composition in the Mangrove Ecosystem of Prieto Diaz, Sorsogon, Philippines ,
Labutap, Noel P./Gonzales-Geronimo, Rhia Odessa/Balatibat, Juancho B./Pampolina, Nelson M., UPLB NRC
Fnesting Technique Using Regional Climate Model Version 3, Badrina, Robert B./Francisco, Raquel dV., UPD
Gene Silencing of Vp9 Gene Impairs WSSV Infectivity on Macrobrachium Rosenbergii, Alenton, Rod Russel R./
Maningas, Mary Beth B., UST
Growth and Characterization of Emeraldine Salt Polyaniline (PANi) Film On Silicon (n-Si) Wafer Substrate By
Chemical Polymerization for Diode Application, Alcantara, Patrick Alvin M./Sambo, Bianca Rae B./Vequizo,
Reynaldo M., MSU-IIT
Heavy Metal Cadmium in Feeds and Tissues of Female Domestic Mallard (Anas Platyrhynchos Domesticus L.) in Victoria,
Laguna and Candaba, Pampanga, Philippines, Octura, Josh Elisha R./Vega, Renato S.A./Sevilla, Cesar C./Tirol,
Ma. Stella C./Capitan, Severino S./Lambio, Angel, UPLB
In Situ Rumen Degradability of Varying Qualities of Basal Diets With Jackfruit By-Product and Soybean Meal
Supplementation in Cattle, Zamora, Fatima A./Bestil, Lolito C., VSU
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
TABLE OF CONTENTS
In Vitro Screening of the Vasorelaxant Activity of Diospyros Discolor Willd. (Ebenaceae) Leaf Extracts, Cadiang, Renz K.
G./Osi, Marina O./Apostol, Jovencio G./Ysrael, Mafel C., UST
Invasion Biology of Suckermouth Armored Catfishes (Pisces: Pterygoplichthys) , Chavez, Joel M./Carandang, Jose
Santos R. VI, DLSU
Isolation and Characterization of the Oil Bodies and Oleosins From the Seeds of Moringa Oleifera Lam., Uy, Abigail
Loren T./Panes, Vivian A., ADMU
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Landmark-Based Geometric Morphometric Analysis On Bodyshape Variation of Mesopristes Cancellatus (Cuvier,
1829), Barazona, Christina A./Torres, Mark Anthony J./Gorospe, Jessie G./Demayo, Cesar G. , MSU-IIT
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Magnetic Field Dependence of the Transition Width of Bi2Sr2-Xmnxcacu2Oy Superconducting Films, Bambao,
Leonalyn V./Sarmago, Roland V., UPD
Mechanized Dry Direct Seeded Rice Social Enterprise Upholding Farmer Fair Trade, Gabunada, Lourd Franz M.,
UPLB
Mineral Resources in the Philippines: Nickel Laterites, Ong, Russel M./Arcilla, Carlo A., UPD
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Norm-Preserving inverse Best Approximation in Hilbert Spaces, Orge, Karlo S./Artes, Rosalio G. Jr., MSU-IIT
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Occurrence of Fungal Foliar Diseases On Selected Mangrove Species in Oil-Spilled Areas in Taklong Island National
Marine Reserve, Nueva Valencia, Guimaras, Philippines, Panaguiton, Diana Dalino/Sadaba, Resurreccion B., UPV
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Pathogenic Yeast Sterilization Via Microwave Atmospheric Pen Plasma (APP), Soriano, Joey Kim T./Del Rosario,
Leo Mendel D./Lacdan, Ma. Camille C./Lee, Henry V. Jr./Ting, Julie Anne S./Ramos, Henry J./Siringan, Maria
Auxilia T./Tumlos, Roy B., UPD
Performance and Meat Qualities of Broiler Fed Diets With Different Levels of Jackfruit (Artocarpus Heterophyllus,
L.) Seed Meal, Gerebise, Ma. Rosa De Lima C./Taveros, Alberto A., VSU
Performance of Growing Lambs As influenced By Liquid Acid Whey Supplementation, Poliquit, Angie R./Sanchez,
Serena L., VSU
Phenotypic and Genetic Characterization of Half-Sib Progenies of Three Eucalyptus (Eucalyptus Deglupta X E.
Pellita) Hybrids in Northern Mindanao, Philippines, Piñon, Albert A./Carandang, Wilfredo M./Fernando,
Edwino S,/Nuevo, Cesar C./Castillo, Manuel L./Galang, Marco A./Quimado, Marilyn O., UPLB
Population Dynamics and Diversity of insects in Pummelo Grown Under Organic and Conventional Pest Management Systems, Aceres, Larry V./Ceniza, M.J.C./Noriel, L.M./Vasquez, E.A./Gonzaga, Z.C., VSU
Relative Warp and Correlation Analysis Based On Distances of the Morphological Shell Shape Patterns of Pomacea
Canaliculata Lamarck From Japan and the Philippines, Moneva, Carlo Stephen O./Torres, Mark Anthony J./
Wada, Takashi/Joshi, Ravindra/Demayo, Cesar G., MSU-IIT
Screening of Actinomycete Isolates for Plant Growth-Promoting Activities (GPA), Cruz, Jayvee A./Paterno, Erlinda
S., UPLB
Seasonal Variability of Meiofaunal Community in A Seagrass Bed in Southern Guimaras, Philippines, Burgos, Lorelie
A./del Norte-Campos, Annabelle, UPV
Sexual Dimorphism in Modularity and integration in the fore and Hind Wing of the Common Evening Brown
Butterfly Melanitisleda Linnaeus 1758 (Nymphalidae: Lepidoptera), Tumang, Vanessa Mae C./Torres, Mark
Anthony J./Demayo, Cesar G., MSU-IIT
Simulation Study of the Filtered Back Projection Method for Proton Computed Tomography of Bone Tissue, Perez,
Ronn Marr M./Nawang, Salasa A., MSU-IIT
Situation Analysis of Solid Waste Management at A Local High School in Region IV: A Benchmark for Establishing
Solid Waste Management Plans in Public High Schools in the Southern Tagalog Region, Pasion, Daryl C/Erasga,
Dennis S., DLSU
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Structural and Superconducting Effect of Doping Manganese On Strontium-Site of Bi2Sr-2Cacu2O8+δ, Santiago,
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The Flory Index of Fractional Brownian Polymer Using Monte Carlo Simulation, Baybayona, Roel N./Cabahug,
Cresente O./Bantayan, Sim P./Bornales, Jinky B./Gemao, Beverly B./Arogancia, Dennis A., MSU-IIT
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Using GIS as Tool Towards a Climate-Disaster Resilient Community: Flood Risk Mapping in Marikina City,
Philippines, Marquez, Teodorico L. Jr./Pulhin, Perlyn M., UPLB
Vomifoliol, A Megastigmane Sesquiterpene From the Leaves of Psychotria Gitingensis Elmer, Eusebio, Jameson A./
Alejandro, Grecebio Jonathan D./Tan, Mario A., UST
Yield and Nutrient Uptake of Lowland Rice As Affected By Application of Chicken Manure Under Different Water
Management Systems, Ratilla, Melanie D./Escinas, Alfredo B., VSU
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
DEPARTMENT OF SCIENCE AND TECHNOLOGY
Office of the Secretary
My warmest greetings to the organizers and participants of the
2nd National DOST-SEI ASTHRDP-NSC Scholar’ Conference.
This conference serves as a good venue by which our graduate scholars
can present their research outputs to the scientific and academic
communities, primarily showcasing their potentials as future researchers
and technologists. With the theme, “Innovations to Accelerate S&T
Human Resource Development”, this event is expected to be an
intellectually stimulating and socially rewarding experience. The presence
of our eminent guest speakers and S&T experts will surely inspire our
participants to actively discuss their research findings.
The Department of Science and Technology (DOST) continues to affirm
its commitment of supporting and providing a proper environment that
will enhance and develop the S&T competitiveness of our nation’s most
vital asset - its human capital.
Science and Technology is associated with modernization and is an
essential tool for a country’s rapid economic progress. It is the centerpiece
of any nation’s development. With the products of science and
technology, people’s lives become comfortable. This is made possible
because of the significant contributions and ingenuity of individuals in
the field of science and technology.
It is hoped that through this annual gathering of graduate scholars in the
various fields of science, engineering and technology, with the able
guidance of their advisers and mentors, our ultimate goal of developing a
culture for research and public appreciation of Science and Technology
will come unto full measure.
To the participants, may this conference motivate you to pursue your
invaluable work in the field of scientific research and bring forth
innovative ideas for practical applications that will result to a better
quality of life of our people. May you always aspire for excellence and the
improvement of your capabilities in your chosen field of expertise.
Congratulations to all the presenters!
Mabuhay tayong lahat!
MARIO G. MONTEJO
Secretary
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
DEPARTMENT OF SCIENCE AND TECHNOLOGY
Office of the Undersecretary for R&D
My warmest congratulations to the Science Education Institute (SEI)
for holding the 2nd National DOST-SEI Accelerated Science and
Technology Human Resource Development Program—National
Science Consortium (ASTHRDP-NSC) Scholars’ Conference.
This activity shows that we value the hardwork and achievements of
our scholars. It shows further that the DOST remains very supportive
of programs/projects aimed at building the nation’s capacity to uplift
the status of science and technology as well as research and
development in the country.
Kudos to all the members of the organizing committee for the success
of this endeavor. Be assured that we shall work hand-in-hand with you
as we continue to develop the future leaders of our beloved country.
Keep up the good work!
AMELIA P. GUEVARA, Ph.D.
Undersecretary for Research &Development
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
SCIENCE EDUCATION INSTITUTE
Office of the Director
My warmest greetings to the members of the National Science
Consortium and the participants of this 2nd National DOST-SEI
ASTHRDP-NSC Scholars’ Conference
In its 2nd serving, this conference hopes to spawn a healthier convergence
among our much valued graduate scholars as we encourage them to go
into research and development (R&D) that shall lead into innovations
that would cater to the needs of our industries.
The dynamic nature of science gives constant pressure to our industries as
to how to adapt to the rapid advancements in technology and the
production process. It is therefore critical that we create a sound scientific
environment where R&D is at its crest in order to ensure the stable rise of
the economy. We are of the firm belief that the participants of this
conference, as evidenced by the kind of works presented in this
conference are competent enough to influence the creation of such type of
scientific environment.
This conference is one of the many ways that the Science Education
Institute supports to create a critical mass of scientists and engineers that
will lead the country in finding S&T-based solutions to the pressing
issues of this time. We hope that through this conference, we are able to
do that and more.
I congratulate all our participants and look forward to seeing you as active
members of the science community.
Thank you and mabuhay!
FILMA G. BRAWNER, Ph.D.
Director
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NATIONAL SCIENCE CONSORTIUM
February 12, 2013
On behalf of the Steering Committee of the National Science Consortium, I warmly
welcome all the faculty, students and guests to the 2nd ASTHRD-NSC Student
Research Conference!
In 2010, the Science Education Institute convened the 10 top science universities in
the country into the National Science Consortium (NSC) to assist it to implement the
Accelerated Science & Technology Human Resource Development Program
(ASTHRD) to fast-track the PhD and MS science scholarship programs of the
country. The institutions which were invited into the NSC accepted this challenge.
The NSC is a large and strategic consortium. All combined, the NSC has more than
700 PhD faculty and offers 33 PhD and 51 MS science degree programs. Currently,
there are about 500 MS and 100 PhD ASTHRD scholars in the NSC. This is a heavy
responsibility, and also a great opportunity to contribute to the development of the
country.
Beyond the management of the scholars, the NSC has taken the challenge to enhance
our science programs in three ways: by improving of the training of our scholars, by
promoting innovation in research, and by forming collaborative programs in strategic
areas. I am pleased to announce that the DOST has responded positively by providing
financial resources so that we can undertake our plans.
This conference will feature two important concerns of the NSC: how to build an
effective consortium and mentorship. Together, these two concerns are keys to a
successful NSC. We are thankful to have with us the best resource speakers on these
topics. Rev. Bienvenido F. Nebres, S.J. and Dr. Ester A. Garcia were two of the pillars
of the first DOST science consortium; Dr. Lourdes Cruz, Dr. Oscar Zamora, and
Dr. Florencia Claveria are exemplars of effective mentors in three areas of science.
Their messages are addressed to both the faculty and scholars of the NSC.
Of course, the main participants of this conference are the scholars. Unlike the usual
conferences, this is an opportunity to share one’s work, test one’s ideas, and, yes, also
to practice and improve. Secondly, this conference is also a unique opportunity for the
NSC to meet and form shared arrangements, whether it is to teach and develop
courses or to undertake collaborative research.
It is our hope that this 2nd Student Research Conference, and its future editions, will
become one of the foundations for a strong Filipino science community!
Sincerely yours,
FABIAN M. DAYRIT, Ph.D.
Chair, NSC Steering Council
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ABOUT ASTHRDP
The Accelerated Science and Technology Human Resource Development Program (ASTHRDP)
is a graduate program being implemented by the Department of Science and Technology (DOST)
through the Science Education Institute and the Councils. The main objective of the program is to help
improve the country’s global competitiveness and capability to innovate through S&T and to
accelerate the production of high-level human resources needed for research and development in S&T.
ABOUT NSC
The National Science Consortium (NSC) is a consortium of ten (10) member-universities,
namely the ADMU, CLSU, DLSU, MSU-IIT, UP-Diliman, UP-Los Baños, UP-Manila, UP-Visayas, UST
and VSU. The NSC was developed to support and coordinate national programs to accelerate the
development of high level human resources in basic, applied sciences and mathematic for the country’s
sustainability and competitiveness.
ASTHRDP
ADVISORY COMMITTEE
NATIONAL SCIENCE CONSORTIUM
STEERING COUNCIL
Chair:
Dr. FABIAN M. DAYRIT
Professor, Department of Chemistry, ADMU
Vice-Chair:
Dr. SOLEDAD M. ROGUEL
Vice President for Academic Affairs, CLSU
Chair:
Engr. MARIO G. MONTEJO
Secretary, DOST
Members:
Dr. AMELIA P. GUEVARA
Undersecretary for R&D, DOST
Dr. ARLENE A. PASCASIO
Dean, College of Science, DLSU
Dr. EVELYN C. CREENCIA
Dean, College of Science and Mathematics, MSU-IIT
Members:
Dr. FILMA G. BRAWNER
Director, SEI-DOST
Dr. JOSE MARIA P. BALMACEDA
Dean, College of Science, UP-Diliman
Dr. ROWENA CRISTINA L. GUEVARA
Executive Director, PCIEERD
Dr. JOSE V. CAMACHO, Jr.
Dean, Graduate School, UP-Los Baños
Dr. JAIME C. MONTOYA
Executive Director, PCHRD
Dr. NINA G. GLORIANI
Dean, College of Public Health, UP-Manila
Dr. PATRICIO S. FAYLON
Executive Director, PCAARRD
Dr. MA. LUISA E. MABUNAY
Dean, College of Arts and Sciences, UP-Visayas
Dr. FABIAN M. DAYRIT
Chair, ASTHRDP-NSC Steering Council
Dr. MARIBEL G. NONATO
Assistant Rector for Research and Innovation, UST
Dr. VICTOR B. ASIO
Dean, College of Agriculture, VSU
13
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
IN RETROSPECT: ASTHRDP IN 2012
1st National DOST-ASTHRDP Scholars’ Conference
February 2012, College of St. Benilde Hotel, Manila
ASTHRDP Orientation and
Signing of the Scholarship Agreement
July 2012, DOST, Bicutan, Taguig City
14
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
IN RETROSPECT: ASTHRDP IN 2012
In Touch with Excellence
July 2012, Hyatt Hotel, Manila
ASTHRDP-NSC Meeting
May 2012, Guimaras
15
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ORAL PRESENTATION SESSION 1
Venue:
Time
Embassy Ballroom 1
Session
Name
1:30 - 1:50
Espita, Dang Marviluz L./Montaño, Marco
S01-1
Nemesio
1:50 - 2:10
S01-2
2:10 - 2:30 S01-3
Title
Quorum Sensing Inhibition By Non-Polar Fractions from Halymenia Durvillaei Bory De
Saint-Vincent From Bolinao, Pangasinan, Philippines
Maini/ZA/Panes/VP/Canlas/MKC/RN, Garcia/ Isolation, Characterization, and Molecular Cloning of A Novel Serine Protease-Like
VS, Tolentino
Gene of the Elastase Sub-Class from Moringa Oleifera Seed Embryos.
Bendoy, Anelyn P./Uy, Mylene M./Vequizo,
Reynaldo M.
School
UPD
ADMU
Synthesis, Morphological, and Structural Characterization of Moistureabsorber Para
MSU-IIT
-Toluenesulfonic Acid-Doped Polyaniline
Sabinay, Stpehen G./Laurena, Antonio C./
2:30 - 2:50 S01-4 Sevilla, Cesar C./Yebron, Medino Gedeun N.
Jr.
Isolation of High Molecular Weight Community DNA From Rumen Digesta for
Metagenomic Studies
2:50 - 3:10 S01-5 Pautong, Putlih Adzra T./Uy, Mylene M.
Cyclic Diarylheptanoid Garugamblin-I Isolated From the Bark of Garuga Floribunda
(“Bogo”)
MSU-IIT
3:10 - 3:30 S01-6 Templonuevo, Xyza Jane/Chakraborty, Soma
Removal of Organic and Inorganic Pollutants From Aqueous System By Chitosan
Molecularly Imprinted Polymer in Modified and Unmodified Form
ADMU
Venue:
Embassy Ballroom 2
Time
Session
1:30 - 1:50
S02-1
Name
Tare, Maria Violeta/Maningas, Mary Beth/
Shitara, Aiko/Kondo, Hidehiro/Hirono, Ikuo
1:50 - 2:10 S02-2 Chavez, Joel
Morillo-Manalo, Laureen/TorrechiliaGuzman, Armi May
Acil, Rachel Y./Diaz, Ma. Genaleen Q./
2:30 - 2:50 S02-4 Amoroso, Victor B./Laude, Rita P./Mendioro,
Merlyn S./Laurena, Antonio C.
2:10 - 2:30 S02-3
2:50 - 3:10 S02-5 Vasquez, Ross D./Ramos, John Donnie
3:10 - 3:30 S02-6
Venue:
Time
1:30 - 1:50
Calma, Rosemarie R./Heralde, Francisco M.
III/Collazo, Margarita C./Schlenk, Daniel
Title
Functional Elucidation of Three Contigs of Marsupenaeus Japonicus to White Spot
Syndrome Virus (WSSV) By dsRNA Inteference
Session
Name
S03-1 Acapulco, Juniven P./Benitez, Julius V.
2:50 - 3:10 S03-5 Kiunisala, Edward M./Jamil, Ferdinand
UST
Taxonomy and New Distributions of invasive Suckermouth Armored Catfishes
(Pisces: Pterygoplichthys)
DLSU
Identification of Mangroves, Seagrasses and Seaweeds of Nogas Island, Anini-Y,
Antique
UPV
Diversity of Some Endemic Nepenthes Species of the Philippines Based on Morphology, rbc L,
mat K, and 18S rDNA Sequences
UPLB
Immunomodulatory and Chemopreventive Properties of Sulfated Polysaccharides
from Sargassum Siliquosum J. G. Agardh
UST
Gene Expressions of Pituitary Adenylate Cyclase Activating Polypeptide (tPACAP38)
and Vitellogenin (Vtg) in Tilapia Exposed to De-71 and 17β- Estradiol Under Freshwater and
Saline Water Conditions
Title
Mcshane and Henstock Equi-integrability of Functions Taking Values in Locally
Convex Topological Vector Space
DLSU
Carlota
Session
S04-1
Name
Linan, Efren L./Ella, Victor B./Florece,
Leonardo L.
Bollozos, Iris/Yniguez, Aletta T./Palermo,
1:50 - 2:10 S04-2 Joseph Dominic/Camoying, Marianne/Lopez,
Mary Rose/Villanoy, Cesar
2:10 - 2:30 S04-3
Gorospe, Jay R C./Juinio-Meñez, Marie
Antonette
2:30 - 2:50 S04-4 Medillo, Japhia V./Ocampo, Virginia R.
School
MSU-IIT
A Spectrally Negative Lévy Risk Process with Piecewise Constant Tax Structure
UPD
Construction and Structural Stability Analysis of Models of Dopamine D1 Receptor
Trafficking in Renal Proximal Tubule Cells
UPD
S-Orbit Colourings of Square and Hexagonal
UPD
In the Complement of A Minimum Dominating Set in the Join, Corona and
Composition of Graphs
3:10 - 3:30 S03-6 Gonzales, Ken M./Manuel Joseph C. Loquias/ Dually-Weighted Stirling-Type Sequences
1:30 - 1:50
School
Ambassador Sala
Veras, Kathleen Zoe E./Sy, Polly W./Valdez,
1:50 - 2:10 S03-2
Adrian Roy
Villar, John Justine S./Arceo, Carlene Perpetua
2:10 - 2:30 S03-3
P./Mendoza, Eduardo R.
Raymundo, Gregorio Abaya III/Baraya-Walo,
2:30 - 2:50 S03-4
Ma. Lailani
Venue:
Time
UPLB
Title
GIS-Based Assessment of Groundwater Vulnerability to Contamination in Boracay
Island Using Drastic Model
MSU-IIT
UPD
School
UPLB
Zooplankton Composition and Abundance in Lamon Bay, Northeast Luzon,
Philippines
UPD
Response of the Juvenile Sea Cucumber Holothuria Scabra To Salinity Stress
UPD
Growth Performance and Nutritional Efficiency of Silkworm (Bombyx Mori L.) Strains
in Sericulture Center, University of the Philippines Los Baños, Laguna
UPLB
Pascual, Evangeline D./Dela Viña, Celia B./
Gregorio, Glenn B./Sajise, Andy/Amas, Junrey/ Mapping the Salinity Tolerance QTL of Hasawi At Seedling Stage Using F6
Arceta, Marydee/Barretto, Aniceta/Vispo, Aiza Recombinant inbred Lines Population
Naireen
3:10 - 3:30 S04-6 Maranguit, Deejay S./Asio, Victor B.
Characteristics and Origin of Soils Along the Central Mountain Range in Leyte
2:50 - 3:10 S04-5
16
UPLB
VSU
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ORAL PRESENTATION SESSION 2
Venue:
Time
Embassy Ballroom 1
Session
Name
Title
School
Bermudez, Stephanie Ann O./Obiles, Romelyn
Electrically Conducting Nis-Montmorillonite Nanocomposite Prepared By
4:00 - 4:20 S05-1 Jane V./Cabañero, Joel M. Jr./Somintac,
Ultrasonic Intercallation and Solid-Solid Reaction
Armando B.
UPD
4:20 - 4:40 S05-2 Chua, Stephanie L./Binag, Christina A.
UST
Graphene / PEDOT-PSS Composites as Supercapacitor Electrode Materials
San Esteban, Anna Carissa M./Enriquez, Erwin Incorporating Graphene in Anthocyanin Dye Sensitizer for Dye-Sensitized Solar
4:40 - 5:00 S05-3
P.
Cells
5:00 - 5:20 S05-4 Monserate, Juvy J./Sumera, Florentino C.
5:20 - 5:40 S05-5
Venue:
Time
Calupitan, Jan Patrick/Enriquez, Erwin P./
Rojas, Nina Rosario L.
Fabrication and Characterization of Poly(Hydroxylauric Acid- e-Caprolactone) –
Clay Nanofiber Composites: A New Potential Scaffold for Tissue Engineering
Functionalization of Glass Substrates for DNA Sensing
ADMU
UPD
ADMU
Embassy Ballroom 2
Session
Name
Title
School
Permejo, Michael Francis P./Singidas, Bess G./
4:00 - 4:20 S06-1 Vargas, Ray M./Sayson, Luce Vida/Sarmago,
Charge Pattern Writing on ZnO Microribbon Using AFM Electric Lithography
Roland V.
UPD
Mahinay, Christian Lorenz S./Malapit,
4:20 - 4:40 S06-2 Giovanni M./Ulano, April M./Poral, Matthew
D./Mascariñas, Venice F./Ramos, Henry J.
Ion Currents of Cu- Ion Beams in a Plasma Sputter Type Negative Ion Source and Its
Correlation With Sputtering and Negative Ion Formation
UPD
4:40 - 5:00 S06-3 Solatorio, Aivin V./Lim, May T.
Complexity in Human Online Communication: Analysis and Modeling of Sending
Patterns in e-Mail and Twitter
UPD
5:00 - 5:20 S06-4
Rico, Christine Adelle L./Alguni, Arnold C./
Vequizo, Reynaldo M.
5:20 - 5:40 S06-5 Hamoy, Efren/Confesor, Mark Nolan
Venue:
Time
Characterization of Silica-Modified Polyaniline and Zinc Oxide Nanocomposites for
Solar Cell Application
MSU-IIT
Experimental Investigation of the Dynamics of a Granular
MSU-IIT
Ambassador Sala
Session
Name
Title
Tremedal, Paul Andrew/David, Anne Marjorie
4:00 - 4:20 S07-1
Isolation and Identification of Seaweed-Associated Carrageenase-Producing Bacteria
E./Lluisma, Arturo O.
School
UPD
4:20 - 4:40 S07-2 Nanca, Carolyn L./Dedeles, Gina Rio
Degradation of Hydrocarbon By Halophilic Bacteria Isolated From Two Local
Salterns in Luzon
UST
4:40 - 5:00 S07-3 Palma, Juvy Ann C./Penuliar, Gil M.
An in Silico Analysis of the Effect of Drug Resistance On Virulence Genes of
Entamoeba Histolytica
UPD
5:00 - 5:20 S07-4
Moron, Llewelyn S./dela Cruz, Thomas Edison Occurrence and Antimicrobial Activities of Fungal Endophytes From Philippine
E.
Mangroves
5:20 - 5:40 S07-5 Vargas, Ma Christina B./Chan, Merab A.
Venue:
Time
Carlota
Session
Name
4:00 - 4:20 S08-1 Dacanay, Aleth Therese L./Osi, Marina O.
Apostol, Jovencio G./Santiago, Librado A./
4:20 - 4:40 S08-2
Ysrael, Mafel Y.
4:40 - 5:00 S08-3
Eda, Rae Abigail R./Diesmos, Arvin C./Chan,
Merab A.
Biodegradation of Untreated, Heat and UV-Treated Polyethylene and OxoBiodegradable Plastics By Bacteria Isolated From Soil
Title
Safety And Bioefficacy Evaluation Of The Essential Oil From Pogostemon Cablin (Blanco)
Benth. (Lamiaceae) Against Aedes Aegypti
Platelet – increasing Property of Euphorbia Hirta Linn. (Euphorbiaceae) in Thrombocytopenic
Rat Models
Cutaneous Bacteria of Frogs Collected From Mt Palay Palay Mataas Na Gulod
National Park, Cavite for Anti-Chytridiomycosis Activity
Castillo, Agnes L./Osi, Marina O./Ramos, John Immunomodulatory and Anti-Scabies Properties of Tinospora Rumphii Boerl Lotion in
5:00 - 5:20 S08-4 Donnie A./De Francia, Jean L./Dyjunco,
Sarcoptes Scabiei Var Hominis-infected Patients and Its Predicted Shelf-Life : A Pilot
Marylaine U./Quilala, Peter F.
Study
5:20 - 5:40 S08-5
Corpuz, Mary Jho-Anne T./Osi, Marina O./
Santiago, Librado A.
Antioxidant and Chemopreventive Properties of Sargassum Siliquosum and its
Preformulation
17
UST
ADMU
School
UST
UST
ADMU
UST
UST
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
POSTER PRESENTATIONS
1
2
3D Reconstruction of Superhydrophobic Surfaces, Buendia, Johanna Evi P./Balili, Ryan B./Candidato, Rolando T.
A Biodiversity Report On the Myxomycete Assemblages in Leaf Litter, Twigs, Dungs, and Inflorescences, Buaya, Anthony
T./dela Cruz, Thomas Edison E.
MSU-IIT
UST
3
Acceptability in Goats and Rumen in Situ Degradability of Foliage With Varying pH Levels From Selected Trees and
Shrubs, Aban, Maita L./Bestil, Lolito C.
4
Analysis of Ethynylestradiol and Levonorgestrel in River Sludge, Enerio, Pio Gerard A./Dayrit, Fabian M.
5
Analytical Calculation of the Radius of Gyration of a Polymer In Fractional Brownian Motion, Bantayan, Sim P./Cabahug,
Cresente O./Baybayon, Roel N./Arogancia, Dennis C./Gemao, Beverly V./Bornales, Jinky B.
6
Biological Control of Pectinolytic, Fruit Soft Rot Agents Using Phylloplane Bacteria, Mateo, Bea C./Dedeles, Gina R.
UST
7
Biosorption On Cadmium (II) By Yeasts From Fruit Peels, Casamorin, Jovy Ann M./Dedeles, Gina R.
UST
8
9
10
Characteristics and Nutrient Status of Highly Weathered Soils in Silago, Southern Leyte, Piamonte, Maria Katrina B./Asio,
Victor B.
Characterization of the Role of Fluctuations in Predator-Prey Dynamics Driven By Demographic Stochasticity, Palenzuela,
Karlo/Juanico, Dranreb Earl
Decadal and Genre Comparison of Musical Networks From Midi Files, Valdez, Nicole/Cabatbat, Josephine Jill/Roxas-
Villanueva, Marianne/Tapang, Giovanni
11
Describing Compartmentalization in the fore-Wing of Populations of the Rice Grasshoppers, Oxya sp. (Acrididae:
Orthoptera), Coronel, Kimverly Hazel I./Torres, Mark Anthony J./Demayo, Cesar G.
12
Design of Electrostatic Ion Accelerator and Focusing System for the Plasma Sputter-Type Negative Ion Source (PSTNIS),
Poral, Matthew D./Ramos, Henry V.
13
DNA Barcoding of the Philippine Traditional and Modern Rice Varieties, Uy, Mary Rose DL/Abella, Evaristo A./Tabanao,
Dindo Agustin A.
14 Duadic Codes Over
15
16
17
, Florida, Veronica B./Dicuangco-Valdez, Lilibeth B./Betty, Rowena Alma L.
Effects of Cultured Yeast Supplementation On Growing Kids Fed With Napier Grass-Concentrate Ration, Abela, Mark V./
Bestil, Lolito C.
Effects of Substrate Orientation on the Growth of Zinc Sulfide Nanostructures, Odarve, Hananish Joy G./Alguno, Arnold
C./Vequizo, Reynaldo M.
Efficiency of the Estimation of the Hurst Exponent in a Time Series Based on Frequency Domain Bootstrapping,
Madronero, Jonathan/Evardone, Chita
18 Ethnobotany of Some Pili (Canarium Ovatum) Varities Native To Gubat, Sorsogon, Chavez, Judith R./Cadiz, Nina M.
19
20
21
22
23
Fabrication of Aluminum Nitride/Air-Gap Distributed Bragg Reflectors From Wet Etching of Sacrificial Aluminum Layers,
Abat, Jonathan Ray R./Mangila, Nemesio S. IV, Salvador, Arnel A./Somintac, Armando S.
Floristic Diversity and Avifaunal Composition in the Mangrove Ecosystem of Prieto Diaz, Sorsogon, Philippines , Labutap,
Noel P./Gonzales-Geronimo, Rhia Odessa/Balatibat, Juancho B./Pampolina, Nelson M.
Fnesting Technique Using Regional Climate Model Version 3, Badrina, Robert B./Francisco, Raquel dV.
Gene Silencing of Vp9 Gene Impairs WSSV Infectivity on Macrobrachium Rosenbergii, Alenton, Rod Russel R./Maningas,
Mary Beth B.
Growth and Characterization of Emeraldine Salt Polyaniline (PANi) Film On Silicon (n-Si) Wafer Substrate By Chemical
Polymerization for Diode Application, Alcantara, Patrick Alvin M./Sambo, Bianca Rae B./Vequizo, Reynaldo M.
Heavy Metal Cadmium in Feeds and Tissues of Female Domestic Mallard (Anas Platyrhynchos Domesticus L.) in Victoria,
24 Laguna and Candaba, Pampanga, Philippines, Octura, Josh Elisha R./Vega, Renato S.A./Sevilla, Cesar C./Tirol, Ma. Stella
VSU
ADMU
MSU-IIT
VSU
ADMU
UPD
MSU-IIT
UPD
CLSU
UPD
VSU
MSU-IIT
MSU-IIT
UPLB
UPD
UPLB
UPD
UST
MSU-IIT
UPLB
C./Capitan, Severino S./Lambio, Angel
25
In Situ Rumen Degradability of Varying Qualities of Basal Diets With Jackfruit By-Product and Soybean Meal
Supplementation in Cattle, Zamora, Fatima A./Bestil, Lolito C.
18
VSU
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
POSTER PRESENTATIONS
26
27
28
29
30
31
In Vitro Screening of the Vasorelaxant Activity of Diospyros Discolor Willd. (Ebenaceae) Leaf Extracts, Cadiang, Renz K. G./Osi,
Marina O./Apostol, Jovencio G./Ysrael, Mafel C.
Invasion Biology of Suckermouth Armored Catfishes (Pisces: Pterygoplichthys) , Chavez, Joel M./Carandang, Jose Santos R.
VI
Isolation and Characterization of the Oil Bodies and Oleosins From the Seeds of Moringa Oleifera Lam., Uy, Abigail Loren T./
Panes, Vivian A.
Landmark-Based Geometric Morphometric Analysis On Bodyshape Variation of Mesopristes Cancellatus (Cuvier, 1829),
Barazona, Christina A./Torres, Mark Anthony J./Gorospe, Jessie G./Demayo, Cesar G.
Magnetic Field Dependence of the Transition Width of Bi2Sr2-Xmnxcacu2Oy Superconducting Films, Bambao, Leonalyn
V./Sarmago, Roland V.
Mechanized Dry Direct Seeded Rice Social Enterprise Upholding Farmer Fair Trade, Gabunada, Lourd Franz M.
32 Mineral Resources in the Philippines: Nickel Laterites, Ong, Russel M./Arcilla, Carlo A.
33 Norm-Preserving inverse Best Approximation in Hilbert Spaces, Orge, Karlo S./Artes, Rosalio G. Jr.
34
35
36
37
Occurrence of Fungal Foliar Diseases On Selected Mangrove Species in Oil-Spilled Areas in Taklong Island National Marine
Reserve, Nueva Valencia, Guimaras, Philippines, Panaguiton, Diana Dalino/Sadaba, Resurreccion B.
Pathogenic Yeast Sterilization Via Microwave Atmospheric Pen Plasma (APP), Soriano, Joey Kim T./Del Rosario, Leo
Mendel D./Lacdan, Ma. Camille C./Lee, Henry V. Jr./Ting, Julie Anne S./Ramos, Henry J./Siringan, Maria Auxilia T./
Performance and Meat Qualities of Broiler Fed Diets With Different Levels of Jackfruit (Artocarpus Heterophyllus, L.) Seed
Meal, Gerebise, Ma. Rosa De Lima C./Taveros, Alberto A.
Performance of Growing Lambs As influenced By Liquid Acid Whey Supplementation, Poliquit, Angie R./Sanchez, Serena
L.
Phenotypic and Genetic Characterization of Half-Sib Progenies of Three Eucalyptus (Eucalyptus Deglupta X E. Pellita) Hybrids
38 in Northern Mindanao, Philippines, Piñon, Albert A./Carandang, Wilfredo M./Fernando, Edwino S,/Nuevo, Cesar C./
UST
DLSU
ADMU
MSU-IIT
UPD
UPLB
UPD
MSU-IIT
UPV
UPD
VSU
VSU
UPLB
Castillo, Manuel L./Galang, Marco A./Quimado, Marilyn O.
39
Population Dynamics and Diversity of insects in Pummelo Grown Under Organic and Conventional Pest Management
Systems, Aceres, Larry V./Ceniza, M.J.C./Noriel, L.M./Vasquez, E.A./Gonzaga, Z.C.
Relative Warp and Correlation Analysis Based On Distances of the Morphological Shell Shape Patterns of Pomacea
40 Canaliculata Lamarck From Japan and the Philippines, Moneva, Carlo Stephen O./Torres, Mark Anthony J./Wada,
VSU
MSU-IIT
Takashi/Joshi, Ravindra/Demayo, Cesar G.
41
42
43
44
45
46
47
48
49
50
Screening of Actinomycete Isolates for Plant Growth-Promoting Activities (GPA), Cruz, Jayvee A./Paterno, Erlinda S.
Seasonal Variability of Meiofaunal Community in A Seagrass Bed in Southern Guimaras, Philippines, Burgos, Lorelie A./
del Norte-Campos, Annabelle
Sexual Dimorphism in Modularity and integration in the fore and Hind Wing of the Common Evening Brown Butterfly
Melanitisleda Linnaeus 1758 (Nymphalidae: Lepidoptera), Tumang, Vanessa Mae C./Torres, Mark Anthony J./Demayo, Cesar G.
Simulation Study of the Filtered Back Projection Method for Proton Computed Tomography of Bone Tissue, Perez, Ronn
Marr M./Nawang, Salasa A.
Situation Analysis of Solid Waste Management at A Local High School in Region IV: A Benchmark for Establishing Solid
Waste Management Plans in Public High Schools in the Southern Tagalog Region, Pasion, Daryl C/Erasga, Dennis S.
Structural and Superconducting Effect of Doping Manganese On Strontium-Site of Bi2Sr-2Cacu2O8+δ, Santiago, Alvin
Carl M./Sarmago, Roland V.
The Flory Index of Fractional Brownian Polymer Using Monte Carlo Simulation, Baybayona, Roel N./Cabahug, Cresente
O./Bantayan, Sim P./Bornales, Jinky B./Gemao, Beverly B./Arogancia, Dennis A.
Using GIS as Tool Towards a Climate-Disaster Resilient Community: Flood Risk Mapping in Marikina City, Philippines,
Marquez, Teodorico L. Jr./Pulhin, Perlyn M.
Vomifoliol, A Megastigmane Sesquiterpene From the Leaves of Psychotria Gitingensis Elmer, Eusebio, Jameson A./Alejandro,
Grecebio Jonathan D./Tan, Mario A.
Yield and Nutrient Uptake of Lowland Rice As Affected By Application of Chicken Manure Under Different Water
Management Systems, Ratilla, Melanie D./Escinas, Alfredo B.
19
UPLB
UPV
MSU-IIT
MSU-IIT
DLSU
UPD
MSU-IIT
UPLB
UST
VSU
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
PLENARY SPEAKERS’ PROFILE
Dr. Ester A. Garcia
Dr. Ester Albano Garcia has been the President of the University of the East (UE) from 2006 to
present and concurrent member of the UE and UERMMMC Board of Trustees and Steering
Committee, South and Southeast Asia and Taiwan Universities Presidents’ Forum. She finished
her bachelor’s degree in Chemistry at the University of the Philippines Diliman, Cum Laude and
PhD degree at the Ohio State University. After which, she has been an international research
fellow of the ICN Nucleic Acid Research Institute in California and Syracuse University. She was
a Professor of the University of the Philippines from 1970 to 2003 and became the Professorial
Lecturer of the same university from 2003 to 2006. She considers her being a faculty member of
the University of the Philippines as her most important achievement in her professional career.
She has published 20 scientific papers in peer-reviewed journals of national and international
circulation, and has written at least 130 other papers on various issues related to science,
education, technology, environment and gender.
Dr. Garcia helped organize two (2) government agencies: the Philippine Council for Advanced Science and Technology
Research and Development (PCASTRD) under the DOST and Commission on Higher Education (CHED). She initiated
scholarship programs in these agencies. She was the Deputy Executive Director of the DOST-PCASTRD in 1988 and later on
became a Commissioner of the Commission on Higher Education (CHED) in 1994 and Chair of the Commission in 1999.
Moreover, Dr. Garcia conceptualized major development projects for CHED, particularly on institutional strengthening funded
by ADB and the strengthening of the whole higher education sector funded under the Higher Education Development Fund
and Mindanao Advanced Education Project and other faculty development programs funded by CHED. The Engineering and
Science Education Project, a World Bank-funded DOST project which supported science and engineering colleges all over the
country was initiated by Dr. Garcia during her years of service in the PCASTRD.
Among the numerous prestigious awards she received were: The National Research Council of the Philippines Achievement
Award in Chemistry; Philippines Federation of Chemistry Societies Achievement Award in Chemical Education; Professional
Regulations Commission Chemistry Professional of the Year in 1996; Government Service Award of the Ateneo De Manila
University in 2003; Doctor of Laws, Honoris Causa of the De La Salle University; Outstanding Women Chemists of the
Philippines during the International Year of Chemistry, 26th Philippine Chemistry Congress in 2011; and Award of Highest
Honor at the Soka Women’s College, Japan, in March 2012.
Fr. Bienvenido F. Nebres, S.J.
Fr. Bienvenido F. Nebres, S.J. served as President of the Ateneo de Manila University for 18 years
1993-2011. He was Dean of the Ateneo College of Arts and Sciences 1973-1980. He was Provincial
Superior of the Jesuits in the Philippines 1983-89 during the time of democratic
transition to
President Corazon Aquino after the years of martial law under President Marcos. He was also
President of Xavier University in Cagayan de Oro in the early 1990s.
He did extensive work to develop science and mathematics in the Philippines and in Southeast
Asia and was elected in 2011 as National Scientist by the National Academy of Science and
Technology (NAST). He was conferred the award by President Benigno Aquino III in September
2011.
After retirement as President, he continues as Professor of Mathematics at the Ateneo de Manila
and is engaged in various programs to help overcome poverty in the country: improving public
education in over 400 public schools with the Ateneo Center for Educational Development (ACED) and in over 200
municipalities, particularly in the Autonomous Region of Muslim Mindanao, with Synergeia Foundation; building homes and
communities for the poor with Gawad Kalinga; and supporting young people engaged in social enterprises for poor
communities.
Fr. Nebres did his early studies in classics and philosophy in the Jesuit Scholasticate, Berchmans College, and completed an
AB and MA in philosophy. He obtained a PhD in mathematics from Stanford University.
20
2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
PLENARY SPEAKERS’ PROFILE
Dr. Lourdes J. Cruz
Dr. Lourdes J. Cruz is a National Scientist and a Professor Emeritus at the Marine Science Institute,
University of the Philippines-Diliman (UPD). She finished her baccalaureate degree in Chemistry at
the UPD in 1962 and pursued her MS and PhD degree in Biochemistry at the University of Iowa, Iowa
City, U.S.A.
Dr. Cruz is concurrent President of the Center for BioMolecular Science Foundation and the Bataan
Center for Innovative S&T, Inc. She was a President of the National Research Council of the in 2012.
She has been a professor of the University of the Philippines-Manila, College of Medicine in 1970;
part-time Research Professor of the University of Utah, Department of Biology in 1987. She has held
various positions in research institutions and the academe.
Because of her achievements as a researcher and educator, she received international and local awards
such as: Sven Brohult Award, International Foundation for Science, Sweden in 1993; Outstanding
ASEAN Scientist and Technologist Award, Brunei Darrusalam in 2001; L’Oreal-UNESCO Award For
Women In Science, Asia Pacific Region (First Filipino & ASEAN to win the award), Paris, France in
2010; Distinguished Alumnus Award, University of Iowa (Roy & Lucille Carver College of Medicine), Iowa City, Iowa, USA in 2011;
Outstanding Young Scientists Award (Chemistry), NAST in 1981; Achievement Award, NRCP in 1982; The Outstanding Women in
the Nation's Service (Science) TOWNS Foundation and Lion's Club (Philippine Chapter) in 1986; and Academician, National
Academy of Science and Technology (NAST) in 1987 among others. She was conferred the Order of National Scientist (Presidential
Proclamation No. 1167, signed on 10 Nov 2006) in 2008. In 2012, she was the Representative of the Philippines in the International
Council of Scientific Unions, Regional Committee for Asia and the Pacific and a Fellow of the Third World Academy of Science.
To date, she has acted as adviser to 9 PhD, 17 MS and 42 BS graduates. She has published a total of 137 papers on different research
works in her field of specialization and hold patents for 12 of her works.
Dr. Evelyn Mae T. Mendoza
Dr. Evelyn Mae Tecson-Mendoza, a member of the National Academy of Science and Technology
(NAST) Philippines since 1992 , is a retired Research Professor and UP Scientist III at the Institute of
Plant Breeding, College of Agriculture, University of the Philippines Los Baños (UPLB). She headed
the Crop Biotechnology Program of the Crop Science Cluster, and was chair of both the BS Agricultural Biotechnology and UPLB Molecular Biology and Biotechnology Program Management Committees. She holds a BS in Chemistry cum laude (Gold Medalist) from the Mapua Institute of Technology
and an MS and PhD in Biochemistry from the University of Massachusetts Amherst. She has worked
extensively on biochemical aspects of Philippine agricultural and underexploited crops published in
more than 100 technical papers in refereed journals. Her more recent researches are on developing
transgenic papaya with long shelf life and virus resistance, protein engineering of mungbean storage
proteins and molecular studies of coconut storage and oil body proteins. A recipient of several
national, professional and institutional awards, she has served as editor of three scientific journals. She
was the Editor-in-Chief of the Philippine Agricultural Scientist (2004-2009) and the Philippine
Journal of Crop Science (1983-1987; 1990-2000) and has been an Overseas Editor of the Bioscience,
Biotechnology and Biochemistry since 2007. With colleagues, she led the development and institution of the MS and PhD Molecular
Biology and Biotechnology Program and the BS Agricultural Biotechnology Program at UPLB.
Dr. Florencia G. Claveria
Dr. Florencia G. Claveria is a full time professor of the Biology Department, College of Science, De La
Salle University-Manila. She obtained her BS in Biology at the St. Louis University in 1973 and MS in
Zoology in Ghent State University, Belgium in 1975. She finished her PhD in Biological Science at the
University of Cincinnati, Ohio, USA in 1987. She has published 67 research papers in manuscripts and
publications. Her research interests are Protozoan zoonotic infections and their vectors (Babesia,
Cryptosporidium, Toxoplasma, Sarcocystis, among others (immunological and parasitological aspects);
Parasitic infections/helminths in farm animals; and Developmental Biology and Avian infections.
Dr. Claveria was a Visiting Professor of the National Research Center for Protozoan Diseases in
Obihiro University, Hokkaido, Japan; Visiting Lecturer of Health Sciences, Henan University, China in
2003; and Post-Doctoral Fellow of the Department of Veterinary Physiology and Protozoan
Immunology, Obihiro University, Hokkaido Japan in 1990.
She is currently the president of the Philippine Society of Parasitology, Inc. She is also a full member of
the National Research Council of the Philippines (NRCP); member of the Governing Board, Center for
Tropical Biology, Bogor, Indonesia, SEAMEO BIOTROP, Editorial Board, Philippine Journal of Science, STII, Department of Science
and Technology, Manila; Chair, Technical Committee of Biology and Molecular Biology, Commission on Higher Education (CHED);
and Member, Technical Panel of Science and Mathematics, Commission on Higher Education TPSM (CHED).
She is a recipient of various awards and distinctions: Grantee, Fulbright-Hays, Ph. D. Biological Sciences, University of Cincinnati,
Ohio, USA (September 1982 to August 1987); Most Outstanding Ph.D. Student, Department of Biological Sciences, University of
Cincinnati in 1987; Grantee, Government of Belgium, M. S. Zoology, Ghent State University (September 1973 to October 1975); St.
Miguel Febres Cordero Award, DLSU-Manila in 2004; Outstanding Teacher Award, College of Science, DLSU-Manila in 1995, among
others.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
PLENARY SPEAKERS’ PROFILE
Dr. Oscar B. Zamora
Dr. Oscar B. Zamora is currently a Professor 12 of the Department of Crop Science and Vice
Chancellor for Instruction of the University of the Philippines-Los Baños (UPLB). He graduated with a
degree in BS Agricultural Chemistry and MS Agronomy at UPLB. He finished his PhD in Crop
Physiology/Agricultural Systems at the University of Queensland, Australia.
He is a member of nine honor/professional societies. He attended, participated and served as resource
person, organizer/co-organizer, facilitator and technical coordinator in at least 75 national and
international conferences/workshops/trainings/meetings in the Philippines, Belgium, Brazil,
Cambodia, Democratic People's Republic of Korea, Germany, India, Indonesia, Italy, Japan, Malaysia,
Singapore, Spain, Sweden, Switzerland, Thailand, The Netherlands and Vietnam. Dr. Zamora is the
Member of the Philippine delegation to the Second Conference of Parties of the Convention on
Biological Diversity (COP) held at Jakarta, Indonesia in 1995 and the 4th FAO International Technical
Conference on Plant Genetic Resources for Food and Agriculture (PGRFA) held at Leipzig, Germany.
He is also the Visiting Professor of the Katholieke Universiteit Leuven, Belgium and University of
Waggeningen University, Netherlands.
He is an Editor in Chief of the Journal of Environmental Science and Management since 2006. His long years of experience in research
and development work on sustainable/alternative agriculture, alternative crop breeding strategy, organic farming, genetic resources
conservation, media development and environment concern in collaboration students, NGO and colleagues in the academe, he has
published as sole author or co-author 22 technical papers in refereed journals; eight magazine articles on sustainable agriculture,
participatory rural development, farmer-scientist partnership, IPR and sui generis system, genetic engineering and food security; and
more than 80 papers presented in national and international conferences (published as proceedings) on topics such as sustainable
agriculture (conceptual, curriculum development and indicators), on-farm conservation of plant genetic resources,
biotechnology/genetic engineering, biosafety, IPR and sui generis system, farmers’ rights and benefit sharing, participatory
development, farmer-scientist partnership for development, food security issues, globalization and liberalization.
Dr. Zamora was a member of the NSC Steering Council representing UPLB from 2010 to May 2012.
Dr. Ludwig Streit
Ludwig P. A. E. Streit is an expert in quantum field theory, quantum mechanics, nuclear physics,
stochastic processes and infinite dimensional analysis, analysis of complex networks and polymer
physics. He finished his education in Doctor of Philosophy from the Univ. Graz, Austria with
distinction in 1962. Dr. Streit has been a “Honorarprofessor” of Graz University in 1972 and Agregação
at the Univ. do Minho, Portugal in 1988.
In his professional career, he has held various positions in the academe in different countries as well as
in research laboratories. Dr. Streit was a professor of the Physics Department of the Syracuse
University, University Bielefeld in Germany and Universidade do Minho. He was appointed as the
Director of various offices such as Center for Interdisciplinary Research, BiBoS Research Center for
Stochastics and Applications in Universidade da Madeira and Centro de Ciências Matemáticas in 1995.
Dr. Streit was a member of the IUPAP Committee for Mathematical Physics in 1981, a member of the
Executive Committee International Association for Mathematical Physics (IAMP) in 1979 and later on
became the Secretary of the IAMP in 1982. He has also served as consultant in many notable projects,
one close to our heart, is his membership in the Scientific Advisory Committee of the Research Center
for Theoretical Physics in Bohol.
As an authority in his areas of specialization, he was involved various editorial activities from 1973 up to 2002. He has also extended
visits to numerous research institutions and universities, and currently, he is a Visiting Professor at the Mindanao State UniversityIligan Institute of Technology. He has mentored research students and fellows from France, India, Malaysia, Nigeria, Austria, Poland,
Portugal, Tunisia, Ukraine and the Philippines. He has also served as referee for scientific journals, books, fellowship, research projects
and hiring committees.
Mr. Andrew Michael S. Ong
Mr. Ong is the former Deputy Director General for Legal, Policy and International Relations of the
Intellectual Property Office of the Philippines. His fixed term appointment ended on December 31,
2012. He is an architect by formal training, having completed his Bachelor of Science in Architecture
from the University of San Carlos in Cebu and topping the Government National Licensure
Examination in 1986, while he practices law, after being called to the Philippine Bar, as a 6th Placer, in
1993, and finishing his Bachelor of Laws from the University of the Philippines. He specializes in
intellectual property and technology law as well as alternative dispute resolution (ADR), more
specifically in negotiation and mediation. He is an accredited mediator of the Court of the Appeals and
qualified patent agent in the Philippines. Besides practicing law and before joining public service in
2009, Mr. Ong occupied several top managerial positions, including project administrator for a major
development project of the Supreme Court of the Philippines on justice reforms. He was the AssistantVice President for Corporate Affairs and Administration for Apo Cement Corporation under J.G.
Summit Holdings, Incorporated, a conglomerate in the Philippines, and Vice-President for Legal and
Administration in Golden ABC Incorporated, a Fashion Brand Company in Asia with a brand portfolio
that includes Penshoppe, Memo, Forme, Regatta, Tyler and Oxygen. Mr. Ong also teaches in the
Philippine Judicial Academy of the Supreme Court of the Philippines and taught at the College of Law and Governance of the
University of San Carlos in Cebu and Ateneo Law School, LL.M. Program. He coauthored the book on trademarks entitled “Protecting
Marks of Ownership.”
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
QUORUM SENSING INHIBITION BY NON-POLAR FRACTIONS FROM HALYMENIA DURVILLAEI BORY DE
SAINT-VINCENT FROM BOLINAO, PANGASINAN, PHILIPPINES
Dang Marviluz L. Espita1,2 and Marco Nemesio E. Montaño1*
1
The Marine Science Institute, Seaweed Chemistry Laboratories and Pilot Plant
Velasquez St., National Science Complex, College of Science, University of the Philippines, Diliman 1101, Quezon City
2
Institute of Chemistry ,National Science Complex, College of Science,
University of the Philippines, Diliman 1101, Quezon City
Email: marviluz@upmsi.ph
Various important biological functions including luminescence, antibiotic response, plasmid transfer, motility,
virulence, biofilmformation and biofouling have been found to be regulated through the interference of bacterial
communication mediated by signal molecules. Theprocess of signal interference, termed as quorum sensing inhibition (QSI),
has brought about researches that screen for lead compounds that are able to block bacterial crosstalk. Bioassays involving the
use of biosensors like the Chromobacteriumviolaceum have provided high-throughput means of screening for bioactive fractions
from several samples. The pigment production of C. violaceum is elicited via a density-dependent cell-to-cell signaling system.
The inhibition of pigment production indicates positive QSI. The availability of this method allows the screening of samples
even at the field, which is advisable for samples of marine origin.
Halymeniadurvillaei is a red marine algae belonging to the Order Cryptonemiales. It is commonly found in rocky
substrates with frequent wave action, and is seldomlyinfestated by epiphytes and other biofouling organisms. This observation
gives insight into its rich phytochemistry that may play an essential in the prevention ofits surface colonization. In this study,
H. durvillaei samples were collected from Bolinao, Pangasinan in Northwestern Luzon, Philippines. Voucher specimens were
deposited to the G.T. Velasquez Herbarium at the UP Marine Science Institute for proper identification and authentication.
Samples for crude extraction were weighed, frozen, and lyophilized prior to extraction. Freeze-dried samples were
homogenized, weighed, and macerated in methanol for 3-5 days. Following solvent extraction, bioassay-guided fractionation
manifests quorum sensing inhibitory activities in DCM and hexane-drivedfractions of H. durvillaei. All experiments were done
in at least triplicate. Current work is on the purification and characterization of the QSI compounds.
ISOLATION, CHARACTERIZATION, AND MOLECULAR CLONING OF A NOVEL SERINE PROTEASE-LIKE GENE
OF THE ELASTASE SUB-CLASS FROM MORINGA OLEIFERA SEED EMBRYOS.
Maini, ZA, Panes, VP*, Canlas, MKC, Garcia RN, Tolentino VS.
Serine proteases are a wide variety of enzymes that hydrolize peptide bonds in proteins and thus have varied
regulatory uses in several members of the plant genera (Antao & Malcata, 2005). In this study, RNA from varying
developmental stages of Moringa oleifera were isolated and subjected to reverse transcription polymerase chain reaction
(RT-PCR) and Random Amplification of cDNA Ends polymerase chain reaction (RACE-PCR) using designed gene-specific
primers. Sequencing results of gel purified amplification products show a 734 and 544 base pair cDNA amplicons with a blastx
(putative amino acid homolgy) score of 83% and 88% similarity to the Homo sapiens Neutrophil Elastase gene (GB ACC78413)
over 155 and 140 translated amino acid residues respectively, both in the (+2) frame over three of nine different embryological
developmental stages. DNA extraction to verify the gene identity of the amplicons is currently in progress, with a 1500 bp
amplicon having been produced using the same gene specific primers. Sequencing and subsequent cloning of the PCR products
will be perfomed. Preliminary data showing resemblance to the neutrophil elastase gene may show potential involvement in
terms of elastin breakdown during seed germination, and may also indicate possible antibacterial and antiviral properties of
the embryo.
SYNTHESIS, MORPHOLOGICAL, AND STRUCTURAL CHARACTERIZATION
OF MOISTURE ABSORBER PARA-TOLUENE SULFONIC ACID-DOPED POLYANILINE
Anelyn P. Bendoy,1 *Mylene M. Uy,1 and *Reynaldo M. Vequizo2
1
Chemistry Department, MSU-Iligan Institute of Technology
2
Physics Department, MSU-Iligan Institute of Technology
A greenish black sample of polyaniline which is indicative of its emeraldine salt form was successfully
synthesized through oxidative chemical polymerization using aniline monomer, ammonium peroxydisulfate as an oxidant and
p-toluene sulfonic acid as acidic medium and dopant. The polymerization process was done inside an inert environment
fabricated gloved box to ensure minimum initial interaction of water molecules to the samples. Dopant concentrations were
varied from 0.2 M to 1.0 M at 0.2 increments.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
Structural characterization of polyaniline samples via Fourier Transform-Infrared (FT-IR) Spectroscopy revealed a
broad absorption peak at ~1115 cm-1 which is assigned C-H in-plane bending an indication that the sample is in an emeraldine
salt form. In addition, it is validated by the calculated values of degree of oxidation (~40%). On the other hand, the sulfonated
polyaniline was confirmed by an absorption peak at ~1034 cm-1 characteristic of SO3- group present in the structure. Hence, a
site for water molecule accommodation is present which is supported by the hollow centred rod-like structure formation
observed in the surface morphology of the sample using Scanning Electron Microscopy (SEM). This is substantiated by the
new peaks observed at ~3239 cm-1 in the FT-IR spectra, indicative of intermolecular hydrogen bonding O-H stretch, which were
obtained after exposure of sample to moisture. Furthermore, increase in weights of the sample was observed after exposure to
moisture. Therefore, pTSA-doped polyaniline is a moisture absorber compound and can be used as a prototype for humidity
sensing materials.
ISOLATION OF HIGH MOLECULAR WEIGHT COMMUNITY DNA
FROM RUMEN DIGESTA FORMETAGENOMIC STUDIES
Stephen G. Sabinay1, Antonio C. Laurena2*, Cesar C. Sevilla3, and Medino Gedeun N. Yebron, Jr.3
1
Physical Sciences Department, West Visayas State University, Iloilo City; 2Biochemistry Laboratory,
2
Institute of Plant Breeding, University of the Philippines Los Baños, Laguna; 3Animal Science Cluster,
University of the Philippines Los Baños, Laguna
Microorganisms in rumen digesta provide a rich source of genes for biotechnological applications. However, these
microbes are difficult to grow in vitro, thus understanding their roles in rumen fermentation remains a challenge. In order to
overcome the limitations of culture-based techniques, metagenomic analysis can be applied. In this study an indirect DNA
extraction method was developed and evaluated by comparing it against four routinely used method designed for soil, bacteria
and plant samples. The modified method facilitates the concentration of microorganims from rumen digesta and employs the
use of bead-beating in the presence of high concentrations of sodium dodecyl sulfate (SDS), salt, and EDTA, with subsequent
purifcation of ammonium acetate and isopropanol. This method resulted in a 2.2- to 4.4-fold increase in DNA yield when
compared to four other widely used method. The community DNA recovered has an estimated maximum size of 9.4- to 23 kb
and was pure enough for restriction digestion and PCR amplification making it ideal for metagenomic library construction and
microbial diversity studies. In addition, the method developed in this study does not use hazardous substances such as phenol
and chloroform nor does it utilize expensive spin columns for purification.
CYCLIC DIARYLHEPTANOID GARUGAMBLIN-I ISOLATED
FROM THE BARK OF GARUGA FLORIBUNDA (“BOGO”)
Mylene M. Uy* and Putlih Adzra T. Pautong
Air-dried bark sample of Garuga floribunda (“Bogo”) was extracted with 95% ethanol and sequentially partitioned
with chloroform. The concentrated chloroform extract (GFC) was fractionated employing gravity column chromatography
(gcc) using gradient mixtures of hexane-ethyl acetate through ethyl acetate-methanol at 10% increment. The eluates were
pooled based on their tlc profiles, resulting to a total of eight fractions which were subjected to brine shrimp lethality test,
with GFC4 showing the highest toxicity (LC50=47.8 ppm).
Purification of GFC4 afforded five sub-fractions (GFC4.1 to GFC4.5), among which GFC4.1 exhibited the highest
toxicity (87.1% mortality) against A. salina. It was then purified, resulting to seven sub-fractions, three of which (GFC4.1.1GFC4.1.3) were sent for NMR spectroscopic analysis. Among the remaining sub-fractions, GFC4.1.5 was further purified
through gcc on the bases of relative purity and weight. This in turn afforded six sub-fractions, with GFC4.1.5.2, GFC4.1.5.5,
and GFC4.1.5.6 subjected to NMR spectral analyses. On the other hand, formation of crystals in GFC3, indicative of high
purity, was the basis for purifying the said fraction. This was done by simply repeatedly washing the crystals with methanol
since they were found to be insoluble in it. The filtrate (dark-green in color) was labeled GFC3.1. Total purification was then
performed by washing the white crystals with hexane, with the filtrate labeled as GFC3.2. The isolated crystals (GFC3.3)
were then submitted for NMR, MS, and X-ray Crystallographic analyses.
Out of the eight sub-fractions sent, only GFC3.3 was completely characterized. Based on spectroscopic analyses, the
identity of the isolate (crystals) was deduced to be garugamblin-I (C21H22O4).
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
REMOVAL OF ORGANIC AND INORGANIC POLLUTANTS FROM AQUEOUS SYSTEM BY CHITOSAN
MOLECULARLY IMPRINTED POLYMER IN MODIFIED AND UNMODIFIED FORM
Xyza Jane Templonuevo and Soma Chakraborty, Ph.D.*
Department of Chemistry, School of Science and Engineering, Ateneo de Manila University, Loyola Heights, Quezon City
Water availability and the ecological and toxicological effects of both organic and inorganic pollutants render water
pollution a major global concern. The target organic pollutant selected for this study is a triacylglyceride (a lipid type) which
can be found in wastewater from slaughterhouses and meat processing facilities. When present in significant amount, lipids
can deplete the amount of dissolved oxygen available for aquatic organisms causing disruption of the aquatic ecosystem such
as loss of aquatic communities by death or migration. An economically attractive mode of water remediation is the use of
chitosan, a biopolymer derived from deacetylation of the structural polysaccharide chitin. Chitin is the second most abundant
natural polymer and can further be obtained by recycling discarded crustacean shell wastes from canning industries.
In this study, molecular imprinting was performed on chitosan to increase its selectivity for the target pollutant.
Chitosan was molecularly imprinted by crosslinking with glutaraldehyde in the presence of triolein (a triacylglyceride) as the
template. Crosslinking was found to be complete after 2h reaction time at 50°C. To increase the interaction of chitosan with
the template, hydrophobic modification was also done by N-acylation using octanoyl chloride. Characterization by FTIR of the
molecularly imprinted and acylated chitosan are currently being performed. Template rebinding will also be investigated as a
function of initial template concentration and contact time. Furthermore, since numerous studies have shown that chitosan
sequesters heavy metal ion pollutants by chelation through its amino groups, the efficiency of the molecularly imprinted
chitosan in simultaneous removal of an organic pollutant (triolein) and an inorganic pollutant (a heavy metal ion) in aqueous
system will also be studied.
FUNCTIONAL ELUCIDATION OF THREE CONTIGS OF MARSUPENAEUS JAPONICUS TO
WHITE SPOT SYNDROME VIRUS (WSSV) BY dsRNA INTERFERENCE
Maria Violeta Tarea, Mary Beth Maningasa,b*, Aiko Shitarac, Hidehiro Kondoc, Ikuo Hironoc
a
Graduate School, University of Santo Tomas, España Manila, Philippines,
b
Department of Biological Science, College of Science, University of Santo Tomas, España, Manila, Philippines,
c
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan
White spot syndrome virus remains to be the main threat in shrimp farming due to its high mortality rate of up to
100% upon infection in 3 to 7 days. To develop strategies to combat the virus, studies on different genes involved in the
host-virus interactions are done to reveal their functions. In this study, contigs 17, 31 and 34 expressed in the genome of kuruma
shrimp was silenced using RNA interference to elucidate its role in the shrimp immune system challenged with WSSV.
Genomic DNA of the shrimp expressed all three contigs, followed by DNA sequencing. Blast analysis revealed that all three are
homologous to the WSSV genome. Mortality data showed that contig 34 provided the highest protection at 25% survival at
day 7 p.i. with the samples in the controls groups PBS and GFP all dead on the same sampling date.
TAXONOMY AND NEW DISTRIBUTIONS OF INVASIVE SUCKERMOUTH
ARMORED CATFISHES (PISCES: Pterygoplichthys)
Joel M. Chavez
Department of Biology, College of Science, De La Salle University
The invasion of suckermouth armoured catfishes (SACs) is one of the more dramatic and traumatic events in
Philippine ecology in recent times. The effects of this invasion have rippled through socio-economic dimensions as reported in
local media that it has left an imprint in the consciousness of many Filipinos on the ill-effects of a lowly aquarium fish fondly
called as “janitor fish.” In this paper, the complications of the mis-identities of the fish are discussed. Discussed as well are the
new areas of distribution for SACs and assessments of their invasion threat levels in these areas.
IDENTIFICATION OF MANGROVES, SEAGRASSES AND SEAWEEDS OF NOGAS ISLAND, ANINI-Y, ANTIQUE
Laureen Morillo-Manalo and Armi May Torrechilia-Guzman*
Institute of Marine Fisheries and Oceanography, College of Fisheries and Ocean Sciences,
University of the Philippines Visayas Miagao, Iloilo
A field survey was conducted in Nogas Island Fish Sanctuary, one of the hundreds of marine protected areas in the
Philippines to identify the species of mangroves, seagrasses and seaweeds present in the area. This survey was conducted on
July 2012 and participated by UP Visayas Fisheries students. Samples of mangroves were collected from the seaward to
landward area while the seagrasses and seaweeds were collected on seagrass beds stretching along the front side of the island.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
Samples were packed in plastic bags and brought to the Fish Biology lab of the University of the Philippines VisayasCollege of Fisheries and Ocean Sciences for identification using field guides and identification keys. Eight mangrove species
were found such as Avicennia marina, Bruguiera cylindrica, Ceriops tagal, Osbornia octodonta, Rhizophora apiculata, Rhizophora mucronata,
Rhizophora stylosa and Sonneratia alba. Five species of seagrasses were also identified which include Cymodocea rotundata, Halodule
pinifolia, Halophila ovalis, Syringodium isoetifolium and Thalassia hemprichii while there were seven species of seaweeds such as
Enteromorpha sp., Gracilaria sp., Halimeda sp., Padina sp., Sargassum sp. and Turbinaria sp. The number of species found in the mangrove
and seagrass beds implies that the island has a diverse and rich habitat which is beneficial for aquatic organisms like fish,
molluscs and crustaceans as these areas are considered as spawning and nursery grounds where these organisms can seek
refuge.
DIVERSITY OF SOME ENDEMIC Nepenthes SPECIES OF THE PHILIPPINES
BASED ON MORPHOLOGY, rbcL, matK, AND 18S rDNA SEQUENCES
Rachel Y. Acil1, Ma. Genaleen Q. Diaz2*, Victor B. Amoroso3*, Rita P. Laude2*,
Merlyn S. Mendioro2*, and Antonio C. Laurena4*
1
Graduate School, University of the Philippines, Los Baños, Laguna, 4031;
2
Genetics and Molecular Biology Division, Institute of Biological Sciences, CAS, UP Los Baños, 4031, Laguna;
3
Department of Biology, Central Mindanao University, Musuan, 8710 Bukidnon;
4
Institute of Plant Breeding, Crop Science Cluster, College of Agriculture, UP Los Baños, 4031, Laguna
The form of resemblance using morphological characters were determined using numerical phenetic analysis; and the
rbcL, matK, and 18s rDNA genes from the thirteen Philippine endemic Nepenthes species were partially sequenced to determine
the extent of diversity of the thirteen endemic Nepenthes species. Numerical phenetic analysis partitioned the thirteen endemic
species into seven distinct groupings. Molecular analysis and DNA barcoding was done using three genes: ribulose-1,5biphosphate carboxylase large sub-unit gene (rbcL), maturase-K (matK), and 18s ribosomal DNA, which were amplified and
sequenced. The samples were all correctly identified up to genus level based on the three genes using nucleotide BLAST.
Genetic distances were computed using Kimura 2-Parameter model and Jukes-Cantor method. The 18s rDNA gene sequences
had the highest genetic divergence, followed by matK and rbcL. The phylogenetic tree based on the concatenated rbcL and matK
sequences formed distinctive clades of the three individuals for each Nepenthes species. Thus, there is morphological and genetic
variation among the thirteen endemic Nepenthes species. This study further proved that the two recommended barcodes, rbcL
and matK, are not powerful enough to discriminate the thirteen Nepenthes species.
IMMUNOMODULATORY AND CHEMOPREVENTIVE PROPERTIES OF SULFATED POLYSACCHARIDES
FROM SARGASSUM SILIQUOSUM J. G. Agardh
Ross D. Vasquez1,4, John Donnie A. Ramos 2,3,4
1
Department of Pharmacy, Faculty of Pharmacy
2
Department of Biology, College of Science
3
Research Center for the Natural and Applied Sciences
4
The Graduate School, University of Santo Tomas, España, Manila
Sulfated polysaccharides from brown seaweeds attracted extensive interest due to their numerous biological
activities and structural diversity. Sulfated polysaccharides isolated from Sargassum siliquosum J. G. Agardh were investigated for
their immunomodulatory and chemopreventive potentials using peripheral blood mononuclear cells (PBMC),
hepatocarcinoma cells (HepG2), renal carcinoma cells (RCC), colon carcinoma cells (HCT) and promyelocytic leukemic cells
(PML) and analyzed using HPLC, Ashing-Acid Digestion Ion Chromatography and FT- IR. Chemical analysis showed that
polysaccharides contain fucose (.35 mM) and mannose (37.762 mM) for AQ; fucose (.363 mM) and mannose (37.762 mM) for
F1 and mannose (1.69 mM) for A1; with IR spectra comparable to standard alginate and fucoidan with sulfate with peaks at
1450-1350 cm-1, O-H stretch with strong signals between 3400-3200 cm-1, C-H stretch at 3000-2850 cm-1 ; C-O at 1300-1000
cm-1, C=O at around 1630-1695 cm-1; degree of sulfation were 11% (AQ), 3.5% (A1) and .21% (A1). AQ, F1 and A1 significantly
induced proliferation of PBMCs and displayed significant antiproliferative activity in both HepG2 and renal carcinoma cells in
vitro in dose-dependent manner and cytostatic effect in human colon cancer cells (p<0.05). ELISA showed that AQ, F1 and A1
significantly inhibited the production of pro-inflammatory cytokines IL-I, IL-6, TNF-ά and monocyte chemotactic protein
(MCP-1) in LPS-stimulated PBMCs and decreased production of NO in LPS-stimulated PML cells. AQ, F1 and A1 showed
concentration-dependent relationship between the amount of sulfate and antioxidant activity in DPPH (IC 50= 1.70 mg/mL
(AQ), 2.095 mg/mL (F1), 2.14 mg/mL (A1); hydroxyl (IC50= 1.037 mg/mL (AQ), 2.08 mg/mL (A1), 2.20 mg/mL (F1) and Nitric
Oxide (IC50= > 2.5 mg/mL) radical scavenging assay (p<0.05). Overall, these results indicated that sulfated polysaccharides of S.
siliquosum possess immunomodulatory and chemopreventive activities and their use as potential natural reagents for cancer
therapy should be given priority.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
GENE EXPRESSIONS OF PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE( tPACAP38 )AND
VITELLOGENIN (Vtg) IN TILAPIA EXPOSED TO DE-71 AND 17β- ESTRADIOL UNDER FRESHWATER
AND SALINE WATER CONDITIONS
Rosemarie R. Calma1, Francisco M. Heralde III*, Margarita C. Collazo2*, Daniel Schlenk3*
1
De La Salle University-Manila and Cavite State University; *University of the Philippines-Manila;
2*
Department of Cell Biology and NeuroScience, University of California-Riverside, U.S.A;
3*
Department of Environmental Sciences, University of California-Riverside, U.S.A.
Polybrominated diphenyl ethers are emerging persistent endocrine disrupting compounds that may adversely affect
the reproductive development of teleost especially under hyperosmotic stress. DE-71 a commercial pentabrominated diphenyl
ether congener with a mild estrogenic property was tested and compared with the effect of 17β-estradiol (E2) on the gonads
and hepatic tissues through the expression of tPACAP38 and Vtg under freshwater and saline water conditions. California O.
mossambicus hybrid eggs were reared in gradual salinity until yolk sac resorption stage then exposed to E2(100 ppm) and DE-71
(2.0 ppm [low], 20 ppm [medium] and 200 ppm [high]) dissolved in acetone through dietary route for 30 days. Total RNA
from cephalic and liver tissues (n = 4 individual fish per group) were isolated and reverse transcribed. Subsequent analysis of the
genes were conducted using One-Step RT-PCR kit and quantified in Real Time PCR in technical duplicates using gene specific
primers designed for tPACAP38 from O. mossambicus (GenBank accession no. AY522580.2), VtgA (GenBank accession no.
EF408235.1), VtgB (GenBank accession no. EF408236.1), VtgC (GenBank accession no. EF408237.1) and normalized with β-actin
(GenBank accession no. AB037865.1) as a reference gene. The cycling threshold values were analyzed via 2∆∆Ct of the Livak
Method. Results showed that tPACAP38 expression in cephalic tissues of tilapia reared in freshwater is upregulated 1x fold in
E2 and low concentration while it is 2x in medium and high concentration compared with the solvent control. Although there
is no significant difference that exists between treatments (p = 0.05), this is an indication of dose-dependent response to
treatments under freshwater condition. When compared with saline water group, tPACAP38 is upregulated 3x as much as in
freshwater group. Its expression is upregulated by DE-71 and E2 under hypersaline condition in a dose-dependent manner (i.e.,
3x fold in E2, 6x folds in medium concentration of DE-71 and 9x folds in high concentration of DE-71). A significant difference
between the control vs. high dose as shown by Dunn’s multiple comparison test (p = 0.0362) with Gaussian approximation is a
remarkable evidence to consider. When both freshwater and saline water group were compared, the expression of tPACAP38 in
cephalic tissues of tilapia exposed in saline water showed also a considerable significance (p = 0.001), although there is no
significant interaction between treatments as shown by 2-way ANOVA. Whereas when each Vtg gene was analyzed
individually, there was no remarkable pattern to show a dose-dependent relationship between treatments and gene
expressions. But when grouped according to salinity level, results showed that salinity level is significantly different (p = 0.023)
for VtgA but there is no interaction with the different treatments except for VtgC which is very significant (treatment p = 0.01 ,
salinity level p = 0.005). These results implied that DE-71 and E2 treatments upregulated the relative gene expression of
tPACAP38, VtgA and VtgC when exposed under saline water condition.
MCSHANE AND HENSTOCK EQUI-INTEGRABILITY OF FUNCTIONS TAKING VALUES
IN LOCALLY CONVEX TOPOLOGICAL VECTOR SPACE
Juniven P. Acapulco1 and Julius V. Benitez2*
1
Department of Mathematics, Mindanao State University-General Santos, Fatima, General Santos City
E-mail: ajuniven@gmail.com
2
Department of Mathematics, Mindanao State University-Iligan Institute of Technology, Tibanga, Iligan City
E-mail: julius.benitez@g.msuiit.edu.ph
In this paper, McShane and Henstock integrals of functions with values in locally convex topological vector space
(LCTVS) X, whose topology is generated by P(X), are defined, where P(X) denotes a family of all continuous seminorms on X.
Some of the basic properties of the McShane and Henstock integrals, such as uniqueness, linearity, Cauchy criterion, and
additive property on subinterval are presented. Some convergence theorems that involve equi-integrability are also considered.
The concepts of inner variation, uniformly strongly differentiability, and uniformly strong Luzin condition are used to
characterize equi-integrability of sequences of integrable functions.
A SPECTRALLY NEGATIVE LÉVY RISK PROCESS WITH PIECEWISE CONSTANT TAX STRUCTURE
Kathleen Zoe E. Veras, *Polly W. Sy, Ph.D. and *Adrian Roy L. Valdez, Ph.D.
Recent advances in the study of ruin problem from the insurance risk theory have generalized the classical CramerLundberg model by considering a spectrally negative Lévy process. Working with this Lévy insurance risk process with a losscarry-forward type of tax payments, we solve using fluctuation theory the two-sided exit problem and the net present value of
tax paid until ruin for two specific tax rate functions. First, we consider a finite number of tax rates which may or may not be
unique and which is a function of time in a finite time horizon. Second, we adapt the tax rate model of Kyprianou and Zhou by
dealing with a piecewise constant tax rate which is a function of the running supremum of our risk process.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
CONSTRUCTION AND STRUCTURAL STABILITY ANALYSIS OF MODELS OF DOPAMINE D 1 RECEPTOR
TRAFFICKING IN RENAL PROXIMAL TUBULE CELLS
John Justine S. Villar1, Carlene Perpetua P. Arceo*2 and Eduardo R. Mendoza*1,2,3
1
Department of Computer Science, University of the Philippines Diliman, Quezon City, Philippines
2
Institute of Mathematics, University of the Philippines Diliman, Quezon City, Philippines
3
Department of Membrane Biochemistry, Max Planck Institute of Biochemistry, Martinsried, Germany
Physicochemical modeling of biochemical processes is in the forefront of systems biology research. However,
reaction mechanisms are difficult to describe, or are even unknown. This poses a major problem in analyzing the properties of
the chemical reaction networks, such as stability and oscillations, which is a common subject in complex diseases such as
hypertension.
Dopamine plays an important role in different physiological and metabolic functions, including the control of
sodium excretion in the kidney. Studies have shown that there is a positive correlation between a defect in dopamine synthesis
and/or dopamine receptor function, and renal sodium excretion, which leads to the development of essential hypertension.
Specific receptors for dopamine, such as the D1 receptor, have been identified in the various regions within the
kidney. It is observed that errors regarding dopamine receptor-G protein coupling and changes in the signaling components
may be responsible for the failure of dopamine to increase sodium excretion in hypertensive subjects.
This study presents kinetic models of dopamine receptor availability and intracellular trafficking, including receptor
activation, desensitization, internalization, degradation and recycling. The first model is a system of differential equations,
called the kinetic equations, which illustrates the behavior of the dopamine receptor system in RPT cells, and the effects of
different mechanisms on its overall dynamics.
Moreover, the second model is a chemical reaction network constructed using Chemical Reaction Network Theory,
a framework that provides different insights regarding the existence of their steady states, as well as their multiplicity and
structural stability. The subsequent analysis finds that the network possesses a unique positive steady state for a given set of
parameters. The proposed models will give an idea on the number of free receptors on the cell surface, as well as the the
number of internalized complexes and receptors at any given time, as well as the effects of various mechanisms that affect cell
surface receptor availability.
S-ORBIT COLOURINGS OF SQUARE AND HEXAGONAL
Gregorio Abaya Raymundo III1 and Ma Lailani Baraya-Walo, PhD*1
Institute of Mathematics, University of the Philippines Diliman
In 2007, de las Peñas and Felix published Color groups associated with square and hexagonal lattices wherein they coloured
different types of square and hexagonal lattices using translations only. They enumerated a list of colour groups and colour-fixing groups associated
with the translation-colouring.
In 2011, Felix and Walo published Colorings of lattices based on subgroup orbits wherein they extended the idea of colouring any
lattice Λ based on orbits of a subgroup S of G, where G is the symmetry group of Λ and S has a finite index inside G. They presented an algorithm in
obtaining the colour group and the colour-fixing group associated with this new method of colouring of a lattice.
For this particular presentation, we demonstrate Felix and Walo’s algorithm by colouring the square lattice
and
the hexagonal lattice
(where k≥2) using orbits of a subgroup S of p4m and p6m (the respective symmetry groups of a square lattice and a
hexagonal lattice). We will also enumerate a list of colour groups and colour-fixing groups associated with this new method of colouring our particular
square and hexagonal lattices.
IN THE COMPLEMENT OF A MINIMUM DOMINATING SET IN THE JOIN,
CORONA AND COMPOSITION OF GRAPHS
Edward M. Kiunisala and Ferdinand P. Jamil*
Mathematics Department, MSU-Iligan Institute of Technology, Iligan City, Philippines
Email: edward k09@yahoo.com; ferdinand.jamil@g.msuiit.edu.ph
The closed neighborhood NG[v] of a vertex v of a graph G is the set consisting of v and all vertices of G which are
adjacent to v. For any SV (G), S is a dominating set in G if V(G) = vϵSNG[v]. If, in addition, S does not contain vertices which
are adjacent in G, S is an independent dominating set in G. Any dominating (respectively, independent dominating) set in G of
minimum cardinality is a minimum dominating (respectively, minimum independent dominating) set in G. An inverse
dominating set in G is a dominating set in G which is a subset of the complement of a minimum dominating set in G. Any
dominating set in G which is in the complement of a minimum independent dominating set in G is a ’i-set in G. In this paper,
we initiate the study of the ’i-sets. We also investigate both the inverse dominating sets and the ’i-sets in the join, corona
and composition of graphs.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
DUALLY-WEIGHTED STIRLING-TYPE SEQUENCES
Ken M. Gonzales1, Manuel Joseph C. Loquias*1 and Roberto B. Corcino*2
1
University of the Philippines, Diliman, Quezon City
2
Mindanao State University, Marawi
The U-Stirling numbers were introduced by Medicis and Leroux as a generalization of q- and p,q-Stirling numbers. In
this talk, we give a further generalization of the U-Stirling numbers called the V-Stirling numbers. These new Stirling-type
sequences are inspired by the symmetric function form of the p,q-binomial coefficients and have combinatorial interpretations
in terms of certain sets of tableaux. Bijections on these tableaux allow us to derive orthogonality relations and convolution
formulas. Lastly, we present a short application of the results and illustrate a few special cases.
GIS-BASED ASSESSMENT OF GROUNDWATER VULNERABILITY TO CONTAMINATION
IN BORACAY ISLAND USING DRASTIC MODEL*
A Special Problem in AEng 290 and a Concise portion of senior author’s dissertation at the School of Environmental Science and Management, UPLB, College, Laguna
Efren L. Linan1, Victor B. Ella2 and Leonardo L., Florece1
1
SESAM, UPLB, College, Laguna
2
CEAT, UPLB, College, Laguna
The study was conducted to assess the vulnerability of groundwater resource to contamination using the DRASTIC
model in combination with Quantum Geographic Information System in Boracay Island, Aklan, Philippines. Specifically, the
study aimed to determine the vulnerability of groundwater resource to contamination by computing its vulnerability index;
develop a groundwater vulnerability map; and recommend policy interventions to sustain the ecotourism industry of the
island. The result showed that indeed the unplanned tourism developments and pressures in the island could lead to the
degradation of groundwater resources and coastal waters. Groundwater resources of Boracay Island that were found to be
vulnerable to contamination were determined as follows: about 410.28 ha (40.87%) were moderately vulnerable; 562.37 ha
(56.01%) were highly vulnerable; and 30.95 ha (3.08%) were considered very highly vulnerable areas in all of the three
barangays of Boracay Island.
The study exhibited the combined use of the DRASTIC
model and Quantum GIS as an effective method for groundwater
contamination vulnerability assessment. It also demonstrated the
cost-effectiveness of the model in identifying vulnerable areas to
contamination and developing vulnerability map (Figure 1) that can
aid in policy making, planning and management interventions to
attain sustainable ecotourism industry in island ecosystem. The
immediate legislation of local ordinance to construct sewerage
system on identified critical areas is recommended to mitigate the
deterioration of aquatic resources in Boracay Island in the future.
Figure 1: Groudwater vulnerability to contamination or the DRASTIC
Index map of Boracay Island generated through GIS
ZOOPLANKTON COMPOSITION AND ABUNDANCE IN LAMON BAY, NORTHEAST LUZON, PHILIPPINES
Iris Bollozos1, Aletta T. Yñiguez1*, Joseph Dominic Palermo1, Marianne Camoying1, Mary Rose Lopez, Cesar Villanoy1*
Marine Science Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines
There has been a lot of interest in understanding the oceanographic and ecological dynamics occurring in the waters
off northeastern Luzon, Philippines. This area is highly influenced by the western boundary current system of the western
Pacific as brought about by the bifurcation of the North Equatorial Current into the Kuroshio and Mindanao Current. Previous
studies have identified high primary productivity in several areas that are said to be driven by upwelling dynamics near the
shelf. These processes are usually linked to productive fisheries. The focus of this study then is on the link between primary
productivity and fisheries, which is zooplankton. Plankton collection was conducted along spatial zones identified a priori
based on chlorophyll a profiles determined from previous studies and oceanographic cruise. The composition and abundance of
general zooplankton groups differed among the spatial zones. Acantharian species were the most abundant in all the samples.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
RESPONSE OF THE JUVENILE SEA CUCUMBER HOLOTHURIA SCABRA TO SALINITY STRESS
Jay R C. Gorospe and Marie Antonette Juinio-Meñez*
Marine Science Institute, Velasquez St., University of the Philippines, Diliman, Quezon City
Responses of the juvenile sea cucumber Holothuriascabra to salinity stress were studied using four different salinity
treatments (35, 29, 25 and 20 ppt) simulated in the hatchery. Hatchery reared juveniles (16.6g±3.76) were exposed to the
salinity treatments for 20 days, afterwhich all treatments were adjusted back to the average ambient salinity (~35 ppt) for
another 20 days.Juveniles exposed to 35, 29 and 25ppt exhibited normal burying behavior. Majority of the animals were at the
surface between 1500H-0300H (44-100%) and were mostly buried between 0300H-1200H (80-95%). On the contrary,
juveniles exposed to 20ppt remained on the surface for the entire duration of the study. Growth rates among treatments were
significantly different (Kruskal-Wallis rank sum test p<0.05) and was highest in the 29ppt (0.45g day -1) followed by juveniles
exposed to 25ppt (0.33g day-1). However post hoc multiple comparison test showed that growth rates between 29 and 25ppt
were not significantly different. Juvenilesat 20 ppt ceased to feed after exposure suggesting physiological stress which
subsequently resulted to the decreasedon their average weightafter 20 days. Survival was 100% in all the treatments except for
20ppt which had 66.7% survival. However, 100% mortality was recorded in 20ppt within 24 hours after the salinity was
adjusted to the average ambient salinity. Results indicate thatH. scabra juveniles can toleratesalinityfluctuationswithin the
range of 35-25ppt and exposure to salinities below 25ppt is physiologically stressful to juveniles.
GROWTH PERFORMANCE AND NUTRITIONAL EFFICIENCY OF SILKWORM (BOMBYX MORI L.) STRAINS
IN SERICULTURE CENTER, UNIVERSITY OF THE PHILIPPINES LOS BAÑOS, LAGUNA
Japhia V. Medillo and Virginia R. Ocampo, PhD*
Crop Protection Cluster, University of the Philippines Los Baños, Laguna
Four silkworm strains used in the study were L7xCN, CNxL7, L7xCJ, CJxL7. The strains were crosses from the three
available parent strains: L7, CN, CJ, in UPLB Sericulture Center. Growth performance was evaluated based on weight, length,
and width of the fifth instar larva until the pupal stage.
The L7xCN and CNxL7 were the strains having higher growth performance rates and nutritional efficiency
parameters. Better growth of silkworm was observed on the month of January.
Similarly, nutritional efficiency of the strains was determined using Alfonso, a mulberry plant variety. Dietary
efficiency and nutrition indices were based on ingesta, digesta, cocoon weight, shell weight, filament weight and length. The
kind and amount of mulberry leaves is important because it affects their overall growth, nutritional need and physiological
changes.
MAPPING THE SALINITY TOLERANCE QTL OF HASAWI AT SEEDLING STAGE
USING F6 RECOMBINANT INBRED LINES POPULATION
Evangeline D. Pascual1,2, Celia B. Dela Viña1*, Glenn B. Gregorio2*, Andy Sajise2,
Junrey Amas2, Marydee Arceta2, Aniceta Barretto2, Aiza Naireen Vispo2
1
University of the Philippines Los Baños,
2
International Rice Research Institute
This study exploited the Hasawi variety for novel sources of QTL for seedling stage salt tolerance using SNP markers.
Phenotypic data for seedling length, biomass, shoot sodium, potassium, and Na +-K+ ratio were taken from the F6 RILs
population. The total size of the linkage map produced from the mapping population using IR29xHasawi parents was 1379.80
cM with an average interval size of 8 cM. A total of 17 significant QTLs have been detected from the population: two for visual
salt injury (qSES1, qSES4); three for shoot length (qSL1, qSL9, qSL12); two for root fresh weight (qRFW4.1, qRFW4.2); three
for root dry weight (qRDW12, qRDW4.1, qRDW4.2); one for percent reduction in root length (qRLRED1); two for shoot fresh
weight (qSFW4 and qSFW12); three for shoot dry weight (qSDW4.1, qSDW4.2, and qSDW7); and one for shoot sodium
concentration (qSNC1). Two large effect QTLs from chromosome 1 have been found for SES (PVE=37.6%) and shoot length
(PVE=41.1%). Four QTL clusters were found in this study: one in chromosome 1 for SES and SNC, two in chromosome 4
responsible for seedling vigour, and one in chromosome 12, also contributing for vigour in the rice plants.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
CHARACTERISTICS AND ORIGIN OF SOILS ALONG THE CENTRAL MOUNTAIN RANGE IN LEYTE
Deejay S. Maranguit, Victor B. Asio*
Soil Science Division, Department of Agronomy and Soil Science, Visayas State University, Baybay City, Leyte 6521-A.
Soil is a natural resource that is very slowly renewable. It is a challenge to protect it, so that it is not depleted faster
than it is renewed. An important prerequisite to effective soil protection is a good understanding of soil origin and
characteristics.
Limited information has been published regarding the characteristics and origin of soils along the central mountain
range in Leyte. This study evaluated the chemical, physical, and morphological properties of soils along the central mountain
range, explain their origin, and determine their fertility status and suitability for crop production. Eight soil profiles
representing the dominant parent materials and physiographic positions in the Abuyog-Mahaplag-Baybay portion of the
central mountain range were studied. The soil profiles were subjected to detailed field and laboratory studies to evaluate their
characteristics and origin.
Results revealed that the soils varied in their morpho-physical and chemical characteristics partly reflecting their
parent material, physiographic positions and to a lesser extent the land-use. In terms of soil development, the soils derived
from shale developed into young (Fluventric Eutropepts) and well-developed (Typic Hapludalfs) soils. The degree of
development appears to be related to the physiographic position. Unstable positions like footslope and shoulder produced
young soils while the more stable positions like summit and backslope produced mature soils. Similarly, the soils derived from
andesite developed into young (Typic Dystropepts) and mature (Typic Kandiudults) soils depending on the physiographic
position.
Land-use appears to have considerably influenced the more dynamic soil properties particularly the nutrient and
organic matter contents. Interestingly, these properties were not very much related to the nature of the parent material and the
physiographic position.
All the soils possessed physical and chemical constraints to crop production which probably explain why most of the
sampling sites were abandoned upland farms. However, the number of constraints present varied among the soils studied. Soil
profiles derived from shale possessed the least number of constraints compared to the other soils indicating that these are the
most suited soils for crop production in the study area.
The study revealed the dominant role of parent material and physiographic position in the characteristics and
development of the soils in the central highland of Leyte island.
ELECTRICALLY CONDUCTING NiS-MONTMORILLONITE NANOCOMPOSITE PREPARED BY ULTRASONIC
INTERCALLATION AND SOLID-SOLID REACTION
Stephanie Ann O. Bermudez1, Romelyn Jane V. Obiles2, Joel M. Cabañero Jr.3 Armando B. Somintac4*
1
National Institute of Physics, University of the Philippines Diliman
2
Materials Science and Engineering Program, University of the Philippines Diliman
3
Institute of Chemistry, University of the Philippines Diliman
This study dealt with providing an efficient method of preparing NiS-montmorillonite nanocomposite with an
electronic conducting property. This was done by two steps. The first step involved an ultrasound-assisted cation-exchange
process between pure montmorillonite and a solution of Ni. The second step was the solid phase reaction between the
prepared Ni-modified montmorillonite and sodium sulfide (Na2S). Characterization of products was done using flame atomic
absorption spectroscopy, scanning electron microscopy, thermogravimetric analysis and direct ohm meter measurements of
resistance. The products showed change in morpholohical structures and electronic properties as montmorillonite has been
intercalated.
GRAPHENE / PEDOT-PSS NANOCOMPOSITES AS SUPERCAPACITOR ELECTRODE MATERIALS
Stephanie L. Chua1,2 and Christina A. Binag1,2*
1
The Graduate School, University of Santo Tomas
2
Research Center for the Natural and Applied Sciences, University of Santo Tomas
Supercapacitors are emerging as alternative power sources that possess high energy density coupled with fast
charge/discharging rates. The capacitive properties of pure poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate)
(PEDOT-PSS) and graphene oxide/PEDOT-PSS composites (GPP) are compared in this study. PEDOT-PSS was polymerized
from 3,4-ethylenedioxythiophene (EDOT) monomer using ammonium persulfate and ferric chloride as oxidant and catalyst,
respectively. GPP composites were prepared by the modified Hummers method followed by in situ polymerization of 3,4ethylenedioxythiophene (EDOT) monomer on graphene oxide dispersion. Electrochemical characterization by cyclic
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
voltammetry (CV) with a potential range of -0.2 V to 0.8 V for 30 cycles at 50 mVs-1 was performed to assess the specific
capacitance and stability of the polymer and composites.PEDOT-PSS and GPP were dispersed in an isopropanol/Nafion
mixture, followed by dropcasting onto a polished glassy carbon electrode. Surface morphology of the synthesized materials
was studied using scanning electron microscopy (SEM).
Cyclic voltammograms of PEDOT-PSS reveal anodic and cathodic peaks at 0.52 V and 0.40 V ascribed to the doping
and dedoping processes of the polymer, respectively. PEDOT-PSS showed a higher specific capacitance in 1M H2SO4 (24.7 Fg-1)
compared to 1M KCl (11.25 Fg-1), yet upon further cycling the PEDOT-PSS modified electrode approaches a rectangular-shaped
voltammogram indicating ideal double-layer capacitance behavior. SEM micrographs of graphite oxide (GO) exhibit folded,
wrinkled paper-like morphology, while PEDOT-PSS displayed globular aggregation. Further characterization of both polymer
and composites are currently ongoing.
INCORPORATING GRAPHENE IN ANTHOCYANIN DYE SENSITIZER FOR DYE-SENSITIZED SOLAR CELLS
Anna Carissa M. San Esteban and Erwin P. Enriquez*
Chemistry Department, School of Science and Engineering, Ateneo de Manila University
The use of natural dyes as sensitizer for dye-sensitized solar cells (DSSC) offers significant advantages such as easy
attainability and cheaper production cost. However, its low power conversion efficiency (PCE) impedes its wide utilization. In
this study, enhancement in efficiency of anthocyanin-based dye sensitized solar cells through the incorporation of graphene
was studied. Graphene is a two-dimensional sheet of sp2-hybridized carbon known for its extraordinary mechanical, thermal
and electrical properties. Different amounts of graphene were added to crude anthocyanin dye extracted from red cabbage. The
resulting mixtures were employed as sensitizer in the fabrication of DSSC. Current-voltage (IV) and Electrochemical
Impedance Spectroscopy (EIS) measurements were carried out to characterize the anthocyanin-based DSSC. The DSSC with
1:2 ratio of anthocyanin-graphene mixture recorded the highest improvement in short circuit current (1.74 mAcm -2) compared
to using anthocyanin dye alone (0.774 mA cm-2). The PCE increased from 0.187% using anthocyanin to 0.436% using
anthocyanin-graphene mixture. EIS data showed a favourable decrease in charge transfer resistance in the TiO 2 layer as
graphene is added to the DSSC. Thus, incorporation of graphene positively affects the performance of DSSC by increasing the
current and decreasing the resistance. The presence of graphene provided better conducting pathways for the photogenerated
electrons.
FABRICATION AND CHARACTERIZATION OF POLY(HYDROXYLAURIC ACID- e-CAPROLACTONE) –CLAY
NANOFIBER COMPOSITES: A NEW POTENTIAL SCAFFOLD FOR TISSUE ENGINEERING
Juvy J. Monserate1,2and Florentino C. Sumera*, 3
The fabrication ofpoly (HOLA-e-CL) nanofiber composites with clay-nanoparticle (ala-MMT) as ring opening
polymerization catalyst were produced via electrospinning method. Effect of various parameter conditions on the
electrospunnanofibersuch as polymer concentration , applied electrical voltage, and tip to collector distance were
investigated. Intercalation and exfoliation of the nano-clay were detected from the change in the XRD pattern (change in the
2Ɵ values), and crystalline structure. Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) were
used respectively to characterize the dispersion of the nano-clay into the nanofiber polymer matrix.
FUNCTIONALIZATION OF GLASS SUBSTRATES FOR DNA SENSING
Jan Patrick Calupitan, Erwin P. Enriquez*, and Nina Rosario L. Rojas*
Department of Chemistry, Loyola Schools, Ateneo de Manila University
Viral diseases such as dengue, avian flu and hepatitis continue to be a major health threat in the Philippines. It was
recognized that early detection of these diseases is essential in preventing serious complications and even death. Advanced
biochemical methods such as Reverse Transcription - Polymerase Chain Reaction were proven effective for early detection of
viral RNA. However, this requires trained personnel, specialized equipment and expensive instruments. This study therefore
seeks to develop a reusable RNA probe for the early detection of viral diseases. In this study, glass substrates are functionalized
with DNA via a streptavidin-biotin linkage. This was confirmed via routine organic qualitative tests and staining protocols
(ninhydrin test and Coomasie dye staining). Meanwhile, gold nanoparticles are functionalized with the complementary strand
of the DNA on the glass by the strong Au-S bond. This was confirmed via UV-Vis spectroscopy. Preliminary results suggest
that reversible hybridization of DNA on the glass surface may be observed optically. This suggests that the material developed
can be used for DNA detection via color changes that could be seen by the naked eye.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
CHARGE PATTERN WRITING ON ZNO MICRORIBBON USING AFM ELECTRIC LITHOGRAPHY
Michael Francis P. Permejo, Bess G. Singidas, Ray M. Vargas, Luce Vida Sayson and Roland V. Sarmago*
Condensed Matter Physics Laboratory, National Institute of Physics,
University of the Philipppines Diliman, Quezon City, 1101
Corresponding author: mfpermejo@gmail.com
Charge pattern writing and reading were done on the surface of ZnO microribbon using AFM electric lithography.
The patterns were written by applying a bias voltage between the cantilever tip and the surface of the ZnO microribbon. For
reading the patterns, they were imaged under Kelvin probe force microscopy (KPFM). As-grown ZnO microribbons and
annealed samples at 600, 700, 800 and 900 were used for pattern writing. Patterns were written on different samples except for
600 ˚C annealed samples. Patterns are observed because of charge accumulation after applying bias voltage on the ZnO
microribbon surface. The charge pattern discharges at ambient temperature and broadening occurs on the charge pattern after
20 minutes. The charge patterns are erased by applying a reverse bias voltage. There is a redistribution of charges after erasing
the charge patterns. This study gives possibility of storing and removing charges on the ZnO surface which is significant for
memory device applications.
ION CURRENTS OF CU- ION BEAMS IN A PLASMA SPUTTER TYPE NEGATIVE ION SOURCE
AND ITS CORRELATION WITH SPUTTERING AND NEGATIVE ION FORMATION
Christian Lorenz S. Mahinay1*, Giovanni M. Malapit2, April M. Ulano3,
Matthew D. Poral4, Venice F. Mascariñas5, Henry J. Ramos6
1,3-6
Plasma Physics Laboratory, National Institute of Physics, University of the Philippines, Diliman, Quezon City
2
Department of Physical Sciences, College of Science, University of the Philippines, Baguio City
*Corresponding author: csmahinay@up.edu.ph
Cu- ion beams are produced in a Plasma Sputter Type Negative Ion Source. An Electrostatic Energy Analyzer (ESA)
is used to sample the negative ion beams with energies that do not exceed 1keV. The corresponding ion currents of the beams
are then calculated. Theoretical values of the sputtering yield and negative ion production probability are calculated per ion
energy value and then compared to the ion current. A correlation value of 0.80021 between the experimental data and
theoretical values suggests a high correlation; therefore, the ion beam is the product of the processes of sputtering and negative
ion formation.
COMPLEXITY IN HUMAN ONLINE COMMUNICATION:
ANALYSIS AND MODELING OF SENDING PATTERNS IN E-MAIL AND TWITTER
Aivin V. Solatorio and May T. Lim
National Institute of Physics
University of the Philippines Diliman
We studied and modeled human online communication using Twitter and e-mail datasets. We found that the
interevent time distribution of sent messages have four regimes. Two of the regimes obey a power law scaling behavior. The
other two regimes are associated with human circadian rhythm and time constraints in composing a message.
CHARACTERIZATION OF SILICA-MODIFIED POLYANILINE AND ZINC OXIDE NANOCOMPOSITES
FOR SOLAR CELL APPLICATION
Christine Adelle L. Rico, Arnold C. Alguno*, and Reynaldo M. Vequizo*
Materials Science Laboratory, Physics Department,
Mindanao State University - Iligan Institute of Technology,
A. Bonifacio Avenue, Tibanga, 9200 Iligan City, Philippines
Conducting organic polymers coupled with inorganic materials as nanocomposites provide interesting applications
in the fields of semiconductors and optoelectronics. For conducting organic polymers, polyaniline has been preferred due to its
higher conductivity, greater environmental stability and ease to prepare. On the other hand, Zinc oxide (ZnO) as inorganic
materials provides excellent tools for crystal growth at nanometer-scale.
Silica-modified polyaniline (SM-PANI) nanostructures were grown on glass substrates via oxidative polymerization
and doping. Likewise, ZnO nanostructures were grown on top of SM-PANI using zinc sulphate and ammonium hydroxide via
chemical bath deposition. Furthermore, concentrations of ammonium hydroxide were varied to 1M and 3M. Scanning
Electron Microscopy (SEM) equipped with Energy Dispersive X-Ray (EDX) Spectroscopy was used to assess the
morphology and elemental composition of the surface of the nanocomposites, and Fourier Transform Infrared (FTIR)
Spectroscopy to analyze its chemical structure.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
SEM-EDX images revealed that SM-PANI rod-like nanostructures are present. Furthermore, growths of sea
urchin-like ZnO nanostructures are very apparent on top of SM-PANI nanostructures. Furthermore, experimental results
showed that ammonium hydroxide concentration can modify the morphology of ZnO nanostructures. It noted that hexagonal
rod-like and sea urchin-like ZnO nanostructures were present for 1M concentration; on the other hand, flake-like structures
were grown for 3M concentration. This might be explained via the competition between growth and etching process during
the synthesis of ZnO at different ammonium hydroxide concentrations. On the other hand, FTIR results suggested that
SM-PANI and ZnO nanostructures were successfully grown as indicated in the signature peaks of the FTIR spectra which
confirmed the surface morphology shown in SEM images.
EXPERIMENTAL INVESTIGATION OF THE DYNAMICS OF A GRANULAR
Efren Hamoy1 and Mark Nolan Confesor 1,2*
1
Department of Physics, MSU - Iligan Institute of Technology, Iligan City 9200, Philippines
2
Department of Physics, National Central University, Jhongli City 320, Taiwan
A polymer in its environment is always bombarded by molecules thereby inducing fluctuations in its conformations.
We show that certain parameters of a macroscopic vibrated granular chain shows characteristics of random motion which is
comparable with the type of motion of polymers. This experiment is a simplified model of how a polymer chain might move in
its environment. Furthermore, a simple model allows us to study and learn about many parameters that are analogous to the
motion of more complex systems.
Granular chains used in this experiment is composed of alternating beads and links which give the granular chain a
certain amount of flexibility. Different lengths of chains were placed on a square wave driven vertically vibrating platform
while vibration strength and frequency kept constant. A camera placed above the platform was used to record as many as 5000
pictures to analyze the motion of the granular chains. Analysis is performed on the series of pictures of the conformations of
the granular chains as it is vibrated on the platform. The center of mass of the granular chains move randomly and from their
initial curled position “relax” very fast and explore the boundaries of the platform. This “relaxation” happens faster in short
chains compared with longer chains. It is possible that given enough time the granular chains will explore the whole area of the
vibrating platform and show all possible conformations.
ISOLATION AND IDENTIFICATION OF SEAWEED-ASSOCIATED CARRAGEENASE-PRODUCING BACTERIA
Paul Andrew A. Tremedal1, Anne Marjorie E. David2, Arturo O. Lluisma2
Bacteria have been suggested to play a role in the occurrence of diseases in seaweeds by producing enzymes that can
degrade structural polysaccharides such as cellulose, alginate and carrageenan. Thus, they are also a potential source of novel
enzymes that may have biotechnological applications. In this study, we isolated and characterized culturable bacteria
from Kappaphycus alvarezii thalli from a seaweed farm in Calatagan, Batangas. Isolates were screened forκ-carrageenase activity
by detection of liquefaction or formation of depression in marine broth solidified with 2% κ-carrageenan. Colonies that have
shown activity were characterized and growth curves were generated One isolate, MGMGL-P03, which demonstrated the
highest enzymatic activity was further characterized. Phylogenetic analysis of its 16s rDNA gene suggests that it belongs to
the CFB (Cytophaga-Flavobacterium-Bacteroides) group, family Flavobacteriaceae andshows the highest similarity with
the species Tenacibaculum mesophilum sp. nov. Suzuki et al., 2001. Few studies have described the species, and only one report on
the phylogeny of this species has been published. This study is the first report of its potential as a κ-carrageenase source.
DEGRADATION OF HYDROCARBON BY HALOPHILIC BACTERIA ISOLATED
FROM TWO LOCAL SALTERNS IN LUZON
Carolyn L. Nanca1,3 and *Gina Rio Dedeles1,2,3
The Graduate School, University of Santo Tomas1, College of Science, University of Santo Tomas2 and Research Center for Natural and Applied
Sciences, University of Santo Tomas3
Polycyclic Aromatic Hydrocarbons (PAHs) are common environmental contaminants which are highly toxic due to
their carcinogenic and mutagenic effects. These are integrated to the environment by incomplete combustion of solid and
liquid fuels, accidental spillage of oils and seep from industrial activities. One of the promising processes of eliminating PAHs
from the environment is through biodegradation of this compound into innocuous product. In the initial screening, a total of
135 halophilic bacteria were obtained from salterns in Dasol, Pangasinan and Noveleta, Cavite. The partial identification and
characterization of these isolates were done through Gram staining and biochemical testing. Of these, 7 bacterial species from
Pangasinan and 5 from Cavite were found tolerant to pyrene, flourene and flouranthene, hence showing a potential to degrade
hydrocarbons. The biodegradation assay followed, by inoculating these bacteria into marine broth with 15% salt and
individually mixed with pyrene, flourene and flouranthene which were then analyzed periodically by UV-Vis
Spectrophotometry until 7 days of incubation. The culture optimization of the best degrader was done using the Taguchi
method. Partial purification of the enzyme responsible for the degradation is also being performed to elucidate the metabolism
of halophilic bacteria in degrading the hydrocarbons.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
AN IN SILICO ANALYSIS OF THE EFFECT OF DRUG RESISTANCE
ON VIRULENCE GENES OF ENTAMOEBA HISTOLYTICA
Juvy Ann C. Palma and Gil M. Penuliar*
U.P. Biology, College of Science, University of the Philippines, Diliman 1101 Quezon City
Entamoeba histolytica is a pathogenic protozoan parasite that causes amoebiasis and is commonly transmitted via
ingestion of cyst-contaminated food and water. The current drug of choice for the treatment of amoebiasis is metronidazole,
although efforts are being made to identify alternative chemotherapeutic agents like trifluoromethionine. Reports of treatment
failure and induction of in vitro resistance, however, have been documented for these two drugs. In this study, the effect of drug
resistance on virulence genes was examined by analyzing microarray data available in public domains. Data sets for
metronidazole resistant (MTZR), trifluoromethionine resistant (TFMR) and wild-type strains were mined from Gene
Expression Omnibus (GEO accession numbers 35990 and 32314, respectively), processed and analyzed. Results showed that
out of 139 virulence genes 34 (24%) and 25 (18%) were differentially regulated by at least two folds in MTZR and TFMR,
respectively. Further analysis indicated that virulence genes in MTZR were predominantly upregulated while those in TFMR
were mostly downregulated. Comparison of the regulated genes revealed that 12 genes were common to both resistant strains.
Overall, the regulation of MTZR and TFMR genes showed very low correlation (R2=0.0403), indicating that drug resistance
affected virulence genes differently in these two resistant strains.
OCCURRENCE AND ANTIMICROBIAL ACTIVITIES OF FUNGAL ENDOPHYTES
FROM PHILIPPINE MANGROVES
Llewelyn S. Moron1 and Thomas Edison E. dela Cruz*2
1
The Graduate School, University of Santo Tomas
Fungal Biodiversity and Systematics Group, Research Center for the Natural and Applied Sciences,
University of Santo Tomas, España, Manila 1015 Philippines2
Mangroves are good hosts for many fungal endophytes. These fungi are potential sources of bioactive metabolites
which can be tapped by our local pharmaceutical companies as promising new drugs. In our research study, we isolated fungal
endophytes from healthy stems and roots of selected Philippine mangroves. Fungal endophytes were isolated from healthy
stems and roots of selected Philippine mangroves. Highest number of fungal endophytes was recorded in Zambales while the
highest number of morphospecies was recorded in Camarines Sur. Forty mangrove fungal endophytes (MFE) were cultured in
vitro and screened for their antimicrobial activities against eight gram-positive and gram-negative bacteria, and two yeasts
using the paper disk diffusion assay. Results showed that 20 of the isolated MFE exhibited inhibitory activities against at least
one of the test microorganisms. Interestingly, three of the MFE were very active against S. aureus (20-22 mm ZOI) and the
yeasts (20-23mm ZOI).Two were very active against B. megaterium (20-23 mm ZOI). Our results showed that inhibitory activity
was more directed towards the gram-positive bacteria and yeasts than gram-negative bacteria. Thin layer chromatography
detected the presence of up to 8 secondary metabolites in the crude culture extracts.
BIODEGRADATION OF UNTREATED, HEAT and UV-TREATED POLYETHYLENE
AND OXO-BIODEGRADABLE PLASTICS BY BACTERIA ISOLATED FROM SOIL
Ma. Christina B. Vargas and Merab A. Chan, Ph.D.*
Department of Biology, School of Science and Engineering
Ateneo de Manila University, Loyola Schools, Loyola Heights, Quezon City 1108, Philippines
The high molecular weight and hydrophobic nature of synthetic polymers
such as plastics render them resistant to direct microbiological attack. Exposure to
abiotic factors such as UV light and heat contribute to initial degradation by
oxidative attack creating scission in the polymer chains. Considering the effect of
abiotic factors and the ability of microorganisms to biodegrade different polymers,
the study aimed to isolate soil bacteria capable of utilizing untreated, UV-A and
heat treated plastics as carbon source. For the abiotic treatment of plastics,
polyethylene and oxo-biodegradable plastic strips were exposed to UV-A (340 nm)
light and heat at 60˚C. Fourier Transform Infrared Spectroscopy was used to
measure Carbonyl Index as a measure of oxidation after abiotic treatment.
Sufficient increase in COi (compared to untreated) was achieved after more than
300 hours of UV-A or heat treatment (Figure 1 and 2). For the isolation of bacteria,
2 grams of soil was enriched in 100 ml synthetic medium containing heat treated
plastics as carbon source. Incubation was done for 4 weeks at 30˚C and 37˚C. After
4 weeks of incubation, isolation was performed using MSM agar with sterilized
small plastic strips as culture medium. Culture plates were incubated at 30˚C for 24
hours.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
SAFETY AND BIOEFFICACY EVALUATION OF THE ESSENTIAL OIL FROM POGOSTEMON CABLIN
(BLANCO) BENTH. (LAMIACEAE) AGAINST AEDES AEGYPTI
Aleth Therese L. Dacanay1,2,3, Marina O. Osi3
University of Santo Tomas Faculty of Pharmacy, Manila, Philippines
2
University of Santo Tomas Research Center for Natural and Applied Sciences, Manila, Philippines,
3
University of Santo Tomas Graduate School, Manila, Philippines
1
Published literatures identified lack of toxicity studies on Pogostemon cablin (Blanco) Benth. (Family, Lamiaceae),
commonly called kablin in the Philippines. Thus, this research sought to bridge the knowledge gap in this area. This
investigation aimed to evaluate potential mosquito repellency following FDA parameters of quality, safety, and bioefficacy.
Pogostemon cablin oil from hydrodistillation of the plant leaves is an aromatic viscous golden yellow liquid. It was
subjected to gas chromatography – mass spectrometry (GC-MS) to determine chromatographic purity. Association between
qualitative analysis and thin layer chromatography (TLC) demonstrated presence of alkaloid, flavonoids, phenols, tannins,
triterpenes and unsaturated sterols. The oil has a pH of about 5.85, specific gravity of 0.9294, refractive index of 1.4369,
viscosity of 25.0 cp, and optical rotation of +32.94°. Acid, saponification, ester, and iodine values were also determined.
Stability dependent on temperature and age, is supported by TLC and pH profiles. It is not photosensitive and
compatible with common lotion and pellet excipients. The foregoing characteristics are described as specifications in a quality
control monograph generated for dosage formulations.
Based on safety guidelines of Organization for Economic Co-operation and Development (OECD), test samples were
a mixture of essential oil and Polysorbate 80 (1:1). Dermal applications to Swiss mice using log dose interval of 0.5 sought to
determine approximate lethal dose concentration. Since there was no sign of toxicity and death, the arbitrary dose of 2000
mg/kg was used as dose level in all succeeding tests.
Dermal Irritation/Corrosion test on Albino rabbits was observed to be non-irritant (Primary Irritation Index =
0.00). Likewise, acute dermal t oxicity test and repeated dose dermal toxicity 28-day study on Sprague-Dawley rats was
negative. Dermal sensitization test using Swiss mice was observed to be non-irritant to the ears of mice and did not promote
ear swelling. Histopathological examination of necropsied organs showed no significant changes. In vitro phototoxicity test
revealed that P. cablin is non-phototoxic.
Bioefficacy test (via human-bait technique) and rapid-screening for larvicidal activity provide evidence-based proofs
that the essential oil is both mosquito repellent and larvicidal.
PLATELET – INCREASING PROPERTY OF EUPHORBIA HIRTA LINN. (EUPHORBIACEAE)
IN THROMBOCYTOPENIC RAT MODELS
Apostol, Jovencio G., Santiago, Librado A.,* and Ysrael, Mafel Y.*
Faculty of Pharmacy, University of Santo Tomas
Reseach Center for the Natural and Applied Sciences, University of Santo Tomas, España, Manila
The decoction of the whole plant of Euphorbia hirta L. (Euphorbiaceae), also known as Australian asthma weed
and, in the Philippines, as tawa-tawa, gatas- gatas or golondrina has a folkloric reputation as a treatment for Dengue Infection
where it is said to hasten convalescence even from the serious stages of the disease. While the chemistry of the plant is
well-studied and other biological effects confirmed by scientific methods, scant evidence in the literature exists about the local
variety and its effects on platelets and other hematologic parameters. This study on the lyophilized decoction of the fresh
whole plant given as 100mg/kg gavage solution revealed platelet- increasing (56%, 50%, 39% in chloramphenicol, heparin, and
ethanol-induced thrombocytopenia,respectively) and bleeding time decreasing (69%, 51%, 60% in chloramphenicol, heparin,
and ethanol-induced thrombocytopenia, respectively) and clotting time- reducing (53%, 49%, 47% in chloramphenicol,
heparin, and ethanol-induced thrombocytopenia, respectively) properties over fourteen days of administration to groups of
male Sprague-Dawley rats rendered thrombocytopenic by the three drugs. Results of Fast Blue BB and Folin-Ciocalteau assays
on the decoction and its Thin Layer and High Performance Liquid chromatograms indicate the possible presence of gallic acid
and catechin. These polyphenols are active compounds that modulate platelet quantity and function putatively through
antioxidant mechanisms that support increase in platelet production and antagonize increase in bleeding and clotting times.
Histopathologic evidence of controlled liver sinusoid dilatation in the E. hirta – treated ethanol-induced thrombocytopenic rats
point to its protective effect in altered platelet distribution. All these results serve to substantiate the popular use of the
decoction of E. hirta in thrombocytopenia possibly due to Dengue Infection and exposure to drugs like Chloramphenicol,
Heparin, and Ethanol.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
CUTANEOUS BACTERIA OF FROGS COLLECTED FROM MT PALAY PALAY MATAAS NA GULOD
NATIONAL PARK, CAVITE FOR ANTI-CHYTRIDIOMYCOSIS ACTIVITY
Adria Rae Abigail R. Eda1, Arvin C. Diesmos2* and Merab A. Chan1*
1
Department of Biology, Ateneo de Manila University
2
Herpetology Section, National Museum of the Philippines
Chytridiomycosis is a disease in amphibians caused by a chytrid fungus Batrachochytrium dendrobatidis and has been
implicated as the causal agent of mass mortality and amphibian extinctions. Mitigating strategies are, thus, among the major
concerns in the conservation of amphibian population and biodiversity worldwide. Reports have shown that microorganisms
found on the skin of amphibians may combat chytridiomycosis. In this study, cutaneous bacteria from frogs were isolated and
examined for anti-chytridiomycosis activity. Eight species of frogs were collected at Mt. Palay Palay Mataas na Gulod National
Park in Cavite - a site positive for the presence of chytrid fungus - including Hylarana similis, Limnonectes woodworthi, Occidozyga
laevis, Platymantis mimulus, P. corrugatus, P. dorsalis, Rhacophorus pardalis and Polypedates leucomystax. Bacteria and chytrid fungus were
isolated from the skin of frogs by swabbing both the ventral and dorsal surfaces of the body and inoculated in R2A agar
incubated at 23±3ºC (Fig. 1). Isolated bacteria were tested for potential inhibitory properties against chytrid fungus.
Preliminary results showed that some cutaneous bacteria of frogs have potential property to inhibit the growth of chytrid
fungus in vitro (Fig. 2). Skin bacteria of frogs may contribute to their defense and immunity against the lethal chytridiomycosis.
Figure 2.Chytrid fungus
inhibition assay. Plate
inoculated with suspected
chytrid fungus; arrows
showing zones of inhibition
produced by three cutaneous
bacterial isolates from frogs.
Figure 1. Cutaneous
bacteria isolated from
the skin swabs of frogs.
IMMUNOMODULATORY AND ANTI-SCABIES PROPERTIES OF TINOSPORS RUMPHII BOERL LOTION
IN SARCOPTES SCABIEI VAR HOMINIS-INFECTED PATIENTS
AND ITS PREDICTED SHELF-LIFE: A PILOT STUDY
Agnes L. Castillo1,2,* Marina O. Osi2 , *John Donnie A. Ramos2,3, Jean L. De Francia4,
Marylaine U. Dyjunco5 and Peter F. Quilala1,6
1
Faculty of Pharmacy, 2The Graduate School, 3College of Science, University of Santo Tomas,
4
Dept of Pediatrics, St.Luke’s Medical Center,5 Dept of Internal Medicine, St. Luke’s Medical Center,
6
Dept of Emergency Medicine, St. Luke’s Medical Center
Scabies is a major public health problem affecting 10% of general population with 50% of these occuring in children.
It is caused by Sarcoptes scabiei mite modulating the host’s inflammatory and immune responses. A randomized, controlled,
single-blind, pilot clinical study was performed to investigate immunomodulatory effect and clinical efficacy of the formulated
Tinospora lotion in sixty-six clinically-diagnosed scabies-infected patients through Enzyme-linked Immunosorbent Assay
(ELISA) for Interleukin-1, Interleukin-6, Interleukin-8 and Monocyte Chemoattractant Protein-1 (MCP-1) in serum samples.
Pediatric patients were treated with Tinospora (test preparation) and Permethrin lotions (control) for three consecutive days
per week for two weeks with blood extraction before treatment, two and four weeks post treatment.
Clinical assessment of each patient was performed every week for five weeks. Tinospora rumphii lotion [Baseline (Mean): 7.20;
After: 0.93; p<0.001] and Permethrin [Baseline: 7.27; After: 0.95; p<0.001] significantly reduced the mean global evaluation score
after four weeks treatment. The two lotions showed comparable clinical improvement [T. rumphii: 0.93; Permethrin: 0.95;
p=0.315] as anti-scabies agent.
Moreover, the clearance time (days) [T. rumphii: 23; Permethrin: 21; p=0.226] and cure rate [T. rumphii: 70%;
Permethrin: 50%; p=0.187] between the two lotions did not differ. Tinospora lotion significantly reduced the IL-1, IL-6, lL-8
levels from Day 14 to Day 28 (p=0.0002, p=0.0002, p=0.0065) which is comparable to Permethrin lotion (p<0.050) with the
exception of MCP (p=0.3497). Such down regulation of Interleukin levels in scabies infestation may be responsible for
hyperkeratosis inhibition, infiltration of inflammatory cells, neutrophil into scabietic lesion and anti-inflammatory effect. The
modulation effect of the Tinospora lotion on interleukin levels potentially reinforces its anti-scabies activity.
Tinospora lotion exhibited physical, microbial and chemical stability at 40˚C storage for 3 months. The lotion is stable
and its predicted shelf-life at 30˚C for 6 months was confirmed. Use of T. rumphii lotion as therapeutic agent for Sarcoptes scabiei
infections is highly recommended.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ANTIOXIDANT AND CHEMOPREVENTIVE PROPERTIES OF SARGASSUM SILIQUOSUM
AND ITS PREFORMULATION
Mary Jho-Anne T. Corpuz12, Marina O. Osi2 and Librado A. Santiago1,2,3
1
Faculty of Pharmacy, 2The Graduate School, University of Santo Tomas
3
Research Center for the Natural and Applied Science, University of Santo Tomas
Antioxidants are intimately involved in the prevention of cellular damage, the common pathway for cancer, aging,
and a variety of diseases. This study aimed to determine the ability of Sargassum siliquosum to prevent the initiation of free
radicals to cause cellular damage. Preformulation studies were also conducted to characterize the crude extract necessary for
standardizing its pharmaceutical quality. The plant was extracted exhaustively using methanol and partition using solvents of
different polarities. Total phenolic contents (TPC) and flavonoid contents (TFC) were evaluated according to Folin-Ciocalteu
and aluminium chloride colorimetric assays, respectively. TPC results showed that there is a significant difference in the mean
gallic acid equivalent (p<0.001) using the five fractions: water < (butanol-water = methanol-water) < hexane < dichloromethane,
in which the mean gallic acid equivalent using dichloromethane is significantly the highest with 69.03 mg/g gallic acid
equivalents (GAE), followed by hexane, methanol-water and butanol-water, then water as the least with 21.26, 15.30, 11.19, and
5.35 mg/g GAE, respectively. TFC demonstrated that there is a significant difference in the mean quercetin (p<0.001) in which
the mean quercetin equivalent using dichloromethane (DCM) is significantly the highest (65 mg/g) compared with other
samples. Radical scavenging activities of the fraction with the highest TPC and TFC were tested against hydroxyl (OH), nitric
oxide (NO) and hydrogen peroxide (H2O2). Results showed that the strongest inhibition was demonstrated by
dichloromethane fraction against radical scavengers OH, NO and H2O2 with % inhibition of 65%, 63%, and 83%, respectively.
The crude extract (pH=5.97) is freely soluble in 95% ethanol and at phosphate buffer pH 10 and pH 12; soluble in 0.01N NaOH,
0.9% NaCl, distilled water and phosphate buffer 1 to 3, 5.5 and 7; sparingly soluble in 0.01N HCl. Qualitative methods and TLC
showed the presence of alkaloids, flavonoids, saponins, tannins, and phenolic compounds. Qualitative methods and thin layer
chromatography showed the presence of alkaloids, flavonoids, saponins, tannins, and phenolic compounds. Stability studies
showed that the degradation of the material is temperature- and age-dependent. The crude extract is photosensitive and
hygroscopic. Thus, it should be stored in a tightly sealed amber glass container at a temperature not higher than 30oC.
Evaluation of the chemopreventive properties of DCM fraction against melanoma cell lines is on going.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
GIS-BASED ASSESSMENT OF GROUNDWATER VULNERABILITY TO CONTAMINATION IN BORACAY ISLAND USING DRASTIC MODEL*
Efren L. Linan, SESAM, UPLB, College, Laguna
Victor B. Ella, CEAT, UPLB, College, Laguna
Leonardo L., Florece, SESAM, UPLB, College, Laguna
*A Special Problem in AEng 290 and a Concise portion of senior author’s dissertation at the School of Environmental Science and Management, UPLB, College, Laguna
Abstract
The study was conducted to assess the vulnerability of groundwater resource to contamination using the DRASTIC model in combination with Quantum Geographic Information System in Boracay Island, Aklan, Philippines.
Specifically, the study aimed to determine the vulnerability of groundwater resource to contamination by computing its vulnerability index; develop a groundwater vulnerability map; and recommend policy interventions to sustain the ecotourism industry of the island. The result showed that indeed the unplanned tourism developments and
pressures in the island could lead to the degradation of groundwater resources and coastal waters. Groundwater
resources of Boracay Island that were found to be vulnerable to contamination were determined as follows: about
410.28 ha (40.87%) were moderately vulnerable; 562.37 ha (56.01%) were highly vulnerable; and 30.95 ha
(3.08%) were considered very highly vulnerable areas in all of the three barangays of Boracay Island.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
3D RECONSTRUCTION OF SUPERHYDROPHOBIC SURFACES
Johanna Evi P. Buendia, Ryan B. Balili* and Rolando T. Candidato*
Physics Department, Mindanao State University – Iligan Institute of Technology,
A. Bonifacio Avenue, Tibanga, 9200 Iligan City, Philippines
E-mail: hanna.buendia@gmail.com
One of the current researches in the photonics Laboratory is the development of micro-patterned superhydrophobic
surfaces – surfaces exhibiting extreme water repellence and with contact angles greater than 150°. The wetting and
hydrodynamic properties of these surfaces can further be explored by examining the micro-patterns and the surface profiles.
In this study, we present a profilometry and surface reconstruction approach adapted to the study of
superhydrophobic surfaces. Using fringe projection, digitally-constructed phase-shifted fringes are projected to a test surface
and the images captured by a camera. The images are processed using phase-shifting techniques to extract the phase values.
From the phase information, the surface profile is calculated using triangulation, and the resulting 3D surface is reconstructed.
A BIODIVERSITY REPORT ON THE MYXOMYCETE ASSEMBLAGES
IN LEAF LITTER, TWIGS, DUNGS, AND INFLORESCENCES
Anthony T. Buaya1,3 and Thomas Edison E. dela Cruz1,2,3
1
The Graduate School, 2 College of Science, and 3Fungal Biodiversity and Systematics Group, Research Center for the Natural and Applied Sciences,
University of Santo Tomas, España, Manila 1015 Philippines
Email: anthonybuaya@gmail.com
Myxomycetes or plasmodial slime molds are a relatively small group of bacterivorous organisms widely distributed in
terrestrial forest ecosystems. Their occurrence in the Philippines was documented in few studies done mainly by foreign
researchers, and quite recently, by Thomasian mycologists. In this study, we looked at the occurrence and diversity of
myxomycetes associated with various organic substrata collected in different localities in Luzon Island, Philippines. Aerial
inflorescences, herbivore dungs, banana aerial leaf litter, mahogany leaf litter, grass leaf litter, coastal twigs, dried fern leaves,
and decayed plant vines were collected and set-up in moist chambers (285). The moist chambers were observed regularly for
the presence of myxomycetes. Species diversity was then assessed using different diversity indices. In our study, 31% of the
moist chambers yielded myxomycetes. More myxomycetes were recorded from fern leaf litter, decayed vines, and from aerial
and ground leaf litter. A total of 19 species belonging to 10 genera were reported in this study: Arcyria afroalpina, A. cinerea, A.
pomiformis, Collaria arcyrionema, Ceratiomyxa fruticulosa, Cribaria violacea, Diderma effusum, D. hemisphaericum, D. squamulosum, L.
scintillians, Perichaena depressa, P. chrysosperma, Physarum cinereum, P. compressum, P. notatum, P. nutans, Stemonitis fusca, and S. nigrescens.
Many of the decayed plant materials collected in the Philippines served as good substrata for myxomycetes.
ACCEPTABILITY IN GOATS AND RUMEN IN SITU DEGRADABILITY OF FOLIAGE
WITH VARYING pH LEVELS FROM SELECTED TREES AND SHRUBS
Maita L. Aban and Lolito C. Bestil*
Visayas State University, Visca, Baybay,Leyte,
Two (2) experiments were conducted to investigate the acceptability of tree/shrub forages as affected by their pH
levels in goats and their consequent rumen degradability and the effects of rumen pH manipulation on the rumen degradation
of such forages. There were several forages analyzed for their pH level and were categorized as low, medium and high pH levels.
Consequently two samples in each category were used in the experiment, and were selected based on the availability of forage
in order to stabilize the supply of forage during the experiment. The six (6) forages tested were Kakawate (Gliricidia sepium),
Madre de agua (Trichanthera gigantea), Acacia (Samanea saman), Gmelina (Gmelina arborea), Robles (Cassia siamea) and Santol
(Sandoricum koetjape). Experiment 1 was set-up in a randomized complete block design (RCBD) considering the various periods
as blocks while experiment 2 was set-up in a completely randomized design (CRD). In situ degradability was conducted by
incubating feed samples in the rumen using the nylon bag technique. Forages with high pH levels that approximate the ideal
level of ruminal pH such as Kakawate and Madre de agua, followed by Gmelina, had significantly higher intakes of dry matter
(DM) both in actual amount and as percent body weight, organic matter (OM) and neutral detergent fiber (NDF) than those
with low pH levels. The pH level of forages significantly influenced dry matter disappearance (DMD) in the first 24 hours of
incubation, such that, forages of high pH levels showed significantly higher values than those with low pH levels. The dry
matter disappearance after 48 hours of incubation appeared to be more affected by the characteristics of the forages influencing
degradability rather than their pH levels. The addition of acetic acid to lower the rumen pH into an ideal level of 6.0-6.4 after
24 hours of incubation increased DM disappearance, indicating the beneficial effects of adding acetic acid into the rumen
within the first 24 hours after feeding.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ANALYSIS OF ETHYNYLESTRADIOL AND LEVONORGESTREL IN RIVER SLUDGE
Pio Gerard A. Enerio and Fabian M. Dayrit
Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
E-mail: fdayrit@ateneo.edu
Ethynylestradiol (EE2) and levonorgestrel (LNor) are common ingredients in oral contraceptive pills. These
compounds are excreted in urine and can have detrimental effects on fish and other aquatic wildlife. It is therefore of interest to
determine their presence in the environment. The analysis of these two compounds has been reported in soil matrix. This study
sought to find the concentration of ethynylestradiol and levonorgestrel in river bed sludge. Sample preparation involved air
drying, accelerated solvent extraction, passage through a solid phase extraction column, and concentration. Analysis with LCMS/MS was performed via standard addition of stable isotope derivatives. However, the low signal-to-noise ratio obtained
prevented accurate quantification.
To explain the low signal-to-noise ratios observed in the river bed sludge matrix, four alcohols will be studied as
surrogates for the hormones under study. These alcohols were chosen to form a series of compounds of increasing bulkiness
and varying shape: beta-napthol, (R)-(+)-1,1'-bi-2-napthol, cholesterol, and 2,4-di-tert-butylphenol. The alcohols will be
converted into their acetyl derivatives using pyridine-acetic anhydride to prepare standards for quantitative measurement. The
alcohols will be spiked separately into river bed sludge and soil, extracted, converted into their acetyl derivatives and analyzed
by GC-MS. The results will hopefully reveal the effect of bulk and shape in the recovery of these analytes in sludge versus soil.
ANALYTICAL CALCULATION OF THE RADIUS OF GYRATION OF A POLYMER
IN FRACTIONAL BROWNIAN MOTION
Sim P. Bantayan, Cresente O. Cabahug, Roel N. Baybayon,
Dennis C. Arogancia*, Beverly V. Gemao*, Jinky B. Bornales*
Physics Department, Mindanao State University-Iligan Institute of Technology,
Bonifacio Avenue, Tibanga, 9200 Iligan City, Philippines
The square of the radius of gyration,
, measures the square distance of the monomers from their center of mass
and is defined more generally not only for polymer chains but also for more complicated structures like polymer brushes, in
which the end-to-end distance, , cannot be directly used for the calculation of macromolecule’s size.
In this paper, the expectation value of
Brownian motion (fBm).
of a single polymer is calculated and extended into the case of fractional
For a polymer in D-dimension with N+1 vertices, the expectation value of
in Brownian case is given by
where is the Brownian path. Since
= DN =
, the final form of equation (1) becomes
=
(N +2)/6(N +1).
Using fBm path
which is dependent in Hurst parameter 0<H<1, integration can be used in (1) in the form of
Evaluating (2) in D-dimension and using the definition
yields,
which gives the relationship of radius of gyration and end-to-end distance in fBm. This reduces to its Brownian counterpart
for H=0.5.
BIOLOGICAL CONTROL OF PECTINOLYTIC, FRUIT SOFT ROT AGENTS USING PHYLLOPLANE BACTERIA
Bea C. Mateo1,3 and *Gina R. Dedeles1,2,3
1
The Graduate School, College of Science,2 Research Center for Natural and Applied Sciences,3 University of Santo Tomas
Foliage microbes possess “special” properties, which allow them to successfully thrive and reproduce in harsh
habitat. Thus, exploiting these potentially bioactive compounds in combating post-harvest soft rot in fruits, specifically its
causative bacterial agent, may resolve significant economic losses locally and elsewhere. The study involved two sampling
phases: (a) isolation, purification and characterization of phylloplane microbes from leaves of ten tomato plants procured from
Manila Seedling Bank; and (b) isolation and purification of pectinolytic bacteria displaying soft rot symptoms following Koch’s
postulate. A total of 240 purified bacteria were isolated from dorsi-ventral sides of leaves, which, 50 were Gram negative and
190 were Gram positive. Dominant bacterial phenotype observed was that of the pigmented type, majority isolated from the
dorsal part of the leaf. The 240 isolates were screened for antagonistic potential using lawn-spotting method. Subsequently,
isolates “positive” for inhibition were subjected to metabolite-based assay using Kirby-Bauer susceptibility method.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
BIOSORPTION ON CADMIUM (II) BY YEASTS FROM FRUIT PEELS
Jovy Ann M. Casamorin; Dr. Gina R. Dedeles*
University of Santo Tomas, Manila 1008, Philippines
The potential of heat-killed yeast biomass from ripe fruit peels was investigated as an efficient biosorbent for
Cd(II). A total of 41 yeast isolates were evaluated for their tolerance to various Cd(II) concentrations. Three species of
Candida (Candida guilliermondii, Candida lusitaniae and six strains of Candida famata) survived the set highest concentration of 500
mg/L of Cd(II). Biosorption ability was found dependent on pH, sorbent dosage, increasing metal concentration and contact
time. The best sorption rate was attained at pH 5 and 6 using 10mg of dead yeast cells after 1-hr contact time. Adsorption rate
of suspended Cd(II) in solution was observed at 92% for 12 and 20ppm. However, only 90% of metal was removed when
concentration was increased to 35ppm. Biosorption of multiple heavy metals [Cd(II), Cr(VI), Pb(II) and Cu(II)] was
measured in vitro using filter-sterilized water samples from Marilao River and 10mg of dead yeasts biomass. Results showed
that rate of adsorption was affected by presence of different metal ions in solution and some of few microorganisms left after
filtration. The ability of dead yeast cells in sorbing metals can be recommended for use in treating wastewaters especially in
developing countries like Philippines.
CHARACTERISTICS AND NUTRIENT STATUS OF HIGHLY WEATHERED SOILS
IN SILAGO, SOUTHERN LEYTE
Maria Katrina B. Piamonte and Victor B. Asio*
Soil Science Division, Department of Soil Science and Soil Science
Visayas State University, Baybay City, Leyte 6521-A
There is a need for detailed soil studies in the mountainous areas of Southern Leyte for the sustainable management
of the soil resources of the province. The study was conducted to evaluate the physical and chemical characteristics and
evaluate the nutrient status of highly weathered soils in Silago, Southern Leyte. Seven soil profiles located along the new
Abuyog-Silago highway were examined and sampled. Soil samples were analyzed for their physical and chemical properties
in the laboratory.
Results indicate that the soils in the study area have closely related properties brought about by closely similar
parent materials and other soil forming factors such as vegetation and climate. The differences in some soil properties appear
to be mainly the effect of topography. In terms of morpho-physical properties, the soils have colors ranging from yellowish
brown to yellowish red, have high clay content (37.04 to 62.15%), and moderate to high porosity values (38.49 to 52.83%).
They are deep (>3 m) and friable when moist but very plastic and very sticky when wet. For the chemical characteristics,
most of the soils have acidic pH values (<6.75), have low to moderate CEC values (11.31 to 38.13 cmol c/kg), low to high base
saturation (0.76 to 69.62%), and extremely low to medium organic matter content (0.07 to 2.59%). Most of the soils are
classified as Typic Hapludults (USDA, 1996) or Haplic Acrisols (WRB, 1998). The soil in the lowest part of the landscape
(toeslope) is classified as Typic Kandiudalfs (USDA, 1996) or Haplic Luvisols (WRB, 1998).
Results also showed that the soils contain low to medium N (0.01 to 0.28%), and extremely low available
P (< 5 mg/kg). However, most of the soils contain sufficient amounts of exchangeable Mg (0.12 to 9.28 cmol c/kg), and Na
(0.05 to 1.70 cmolc/kg) except profile 5, but deficient in Ca (0.07 to 0.27 cmolc/kg) and exchangeable K (0.02 to 0.37
cmolc/kg) since the soils are strongly acidic. The soils also contain high amounts of dithionite-extractable Fe (0.02 to 1.28
g/kg) indicating the abundance of crystalline and non-crystalline iron oxides. In terms of relationships among soil properties,
only organic matter and total N are positively correlated.
All the soils possess several physical and chemical constraints to crop production, which are related to the
advanced stage of soil development. The nutrient status of the soils is also related to the very low pH values, composition of
the parent material, some anthropogenic factors, and topographic position of the soils.
CHARACTERIZATION OF THE ROLE OF FLUCTUATIONS IN PREDATOR-PREY DYNAMICS
DRIVEN BY DEMOGRAPHIC STOCHASTICITY
Karlo Palenzuela and Dr. Dranreb Earl Juanico*
Department of Mathematics, School of Science and Engineering
Ateneo de Manila University, Loyola Heights, Quezon City 1108
The objectives of this paper are to model, simulate and analyze stochastic fluctuations of predator-prey dynamics
by resorting to van Kampen system-size expansion and Gillespie algorithm. The master equation has been formulated and
use the perturbation method proposed by van Kampen which provides the Fokker-Planck equation describing the time
evolution of the unknown probability distribution governing the statistics of the fluctuations. The Gillespie algorithm was
used to simulate the master equations that denote the transition of the state of the system according to a Markov process.
These two approaches in stochastic analysis proved to provide a more detailed description of the system.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
DECADAL AND GENRE COMPARISON OF MUSICAL NETWORKS FROM MIDI FILES
Nicole Valdez*, Josephine Jill Cabatbat, Marianne Roxas-Villanueva and Giovanni Tapang
Instrumentation Physics Laboratory, National Institute of Physics,
University of the Philippines, Diliman, Quezon City
*Corresponding author: nvaldez@nip.upd.edu.ph
In the present study, we obtained MIDI songs from (i) ten composers each from three genre – rock, jazz and classical
music – and from (ii) a ten year period of three genre – rock, pop and country music. and converted the songs into musical
networks. Betweenness centrality, clustering coefficient, degree and radius have been computed and compared. Classical music
networks has been observed to obtain high betweenness centrality and radius while rock music networks has been observed to
have a high clustering coeffcient. These network parameters give information on the structure of the network and structure of
the song. For the comparison of music networks over time, no significant difference in the network properties for rock and pop
music. However, the betweenness centrality and radius of country music networks was observed to be consistently higher
through the years.
DESCRIBING COMPARTMENTALIZATION IN THE FORE-WING OF POPULATIONS
OF THE RICE GRASSHOPPERS, Oxya sp. (ACRIDIDAE: ORTHOPTERA)
1
Kimverly Hazel I. Coronel, 1Mark Anthony J. Torres* and 1Cesar G. Demayo*
1
Department of Biological Sciences, College of Science and Mathematics,
Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines
The fore-wings of Rice grasshoppers, Oxya sp. are divided into compartments by a complex network of veins. There
has been a long term hypothesis suggesting that wing compartments bounded by veins may correspond to distinct cell lineages
and domains of gene expression. Hence, this study was conducted to determine the patterns of modularity and integration in
the fore-wings of the Rice grasshoppers, Oxya sp. A total of 180 points were used to outline the margins of the fore-wings.
Results showed that the fore-wing of Oxya sp. is partitioned into 6 developmental modules. The consistency in the number and
pattern of developmental modules in the fore-wings suggests that the wings are highly conserved indicating genetic
conservatism in the morphological spaces. Results also suggest that compartments are considered an autonomous unit of gene
regulation and major veins served as boundaries and as active center of integration.
DESIGN OF ELECTROSTATIC ION ACCELERATOR AND FOCUSING SYSTEM
FOR THE PLASMA SPUTTER-TYPE NEGATIVE ION SOURCE (PSTNIS)
Matthew D. Poral, Henry V. Ramos*
Plasma Physics Laboratory, National Institute of Physics, College of Science,
University of the Philippines – Diliman, Quezon City
The Plasma Sputter-Type Negative Ion Source (PSTNIS) facility of the Plasma Physics laboratory is now capable of
producing negative metal ions (e.g. Zr -, Ag -, Au -, Cu –, Ti -) and measurement systems for the corresponding beam properties
such as beam energy spread, beam current, beam charge density and beam width are already functional. The next step is to
fabricate a beam accelerator and focusing component that will enhance the beam energy and intensity. The aim of this study is
to conceptually design an electrostatic ion accelerator and focusing system based on theoretical ion optics and computational
methods.
DNA BARCODING OF PHILIPPINE TRADITIONAL AND MODERN RICE VARIETIES
Mary Rose DL Uy, Dr. Evaristo A. Abella* and Dr. Dindo Agustin A. Tabanao*
DNA barcoding is a method for reconstructing the taxonomic classification at the molecular level through gathering
sequences of traditional and modern rice varieties as part of the government’s intention to protect these varieties from being
owned by other countries. This study was undertaken to obtain a DNA barcode from traditional and modern rice varieties,
using standard markers set forth by the International Barcode of Life. Chloroplast loci were used in resolving varietal genotype
differences within traditional and modern rice varieties. The three loci: rbcL, matK and trnH-psbA were evaluated in terms of
ease of amplification and sequencing, sequence quality and discriminatory power. All exhibited 100% PCR amplification and
sequencing success across 70 rice cultivars. The DNA sequences from different varieties were analyzed using Mafft alignment
procedure in Jalview. Results showed highest frequency of SNPs in matK locus. To estimate nucleotide diversity and genetic
relationships among traditional and modern rice varieties, parameters of estimates of nucleotide diversity were calculated.
Results revealed matK locus obtained the highest nucleotide diversity among traditional and modern varieties with θ= 0.01828.
Also, matK locus obtained the highest nucleotide diversity within traditional and modern rice varieties with θ= 0.02086.
Neutrality tests were investigated by calculating Tajima’s D and Fu and Li’s D* and F* values. All yielded negative values
implying a bias towards an excess of rare alleles in the population which may indicate recent population growth and selection.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
Phylogenetic analysis revealed that each locus was not able to discriminate 70 rice cultivars. A multi-locus analysis produced a
more resolved tree than single locus analysis. It is suggested that additional markers must be used to increase discriminatory
power within species level. As a benchmark study in barcoding of Philippine rice varieties, these data can be used in further
standardization and establishment of DNA barcodes and will provide significant information that can be utilized in plant
breeding purposes in rice varieties.
DUADIC CODES OVER
Veronica B. Florida1, Lilibeth B. Dicuangco-Valdez*2 and Rowena Alma L. Betty*2
1
Department of Mathematics, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City
2
Institute of Mathematics,University of the Philippines-Diliman, Quezon City
Duadic codes over
, the function field analogue of duadicZ4-codes, were introduced as abelian codes by
Langevin and Solé. In this study, we extend the notion of duadic codes to the context of abelian codesover
, where
and an integer. We study these codes by analogy with
case. Group ring structures of codes over
are investigated. We describe the properties, self-duality, augmented andextended codes of a generalized duadic codes over
.
EFFECTS OF CULTURED YEAST SUPPLEMENTATION ON GROWING KIDS
FED WITH NAPIER GRASS-CONCENTRATE RATION
Mark V. Abela and Lolito C. Bestil*
Department of Animal Science, Visayas State University, Visca, Baybay City, Leyte
The study was conducted to evaluate the effects of cultured yeast supplement (Saccharomyces cerevisiae) on dry matter
intake (DMI), growth performance, fluctuation in ruminal fluid pH and rumen bacterial count (cfu/mL), and on the
digestibilities of dry matter (DM), organic matter (OM), and crude protein (CP) of napier grass-concentrate ration. Twelve
growing kids were distributed to the three (3) treatments with four (4) replicates arranged in a randomized complete block
design as follows: 1) control, without yeast supplement, 2) treatment one, T 1, with one (1) gram cultured yeast in 10 mL
distilled water given at 2 mL per head/day, and 3) treatment two, T2, with three (3) grams cultured yeast in 10 mL distilled
water given at 2 mL per head/day.
The addition of cultured yeast generally increased DM intake (p<0.003), weight gain (p<0.006), and ruminal pH
(p<0.045), with T2 showing higher values, but had no significant effects on bacterial count (p>0.435) among treatment groups.
Digestibilities of DM (p<0.002), OM (p<0.000), and CP (p<0.000) of napier grass-concentrate ration were increased by the
addition of cultured yeast at both levels of supplementation, with T 2 group showing higher values. In conclusion, yeast
supplementation enhanced nutrient intake and utilization of the napier grass-concentrate ration, dramatically increased
ruminal pH of goats into optimum levels necessary for the growth and activity of rumen microorganisms which eventually
enhanced fiber digestion.
EFFECTS OF SUBSTRATE ORIENTATION ON THE GROWTH OF ZINC SULFIDE NANOSTRUCTURES
Hananish Joy G. Odarve, Arnold C. Alguno* and Reynaldo M. Vequizo*
Materials Science Laboratory, Department of Physics,
Mindanao State University – Iligan Institute of Technology, A. Bonifacio Avenue, Tibanga, 9200 Iligan City, Philippines
Nanoscale semiconductor materials received much attention in the scientific field because it exhibits unique
properties that can be utilized in various optoelectronic applications. It is widely known that semiconductor industry
generally utilized silicon. Integrating nanoscale materials into silicon substrates is a great challenge to enhance the
performance of today’s semiconductor devices. Zinc sulfide (ZnS) is one of the promising materials to be integrated into
silicon. Several methods and techniques have been used in order to prepare ZnS nanostructures. However, chemical bath
deposition (CBD) method is better as compared to other methods due to its simplicity and easy to control in the growth
process as well as its low processing cost. In this study, ZnS nanostructures grown on Si substrates with different orientation,
Si(100) and Si(111), are synthesized via CBD method. Zinc chloride (ZnCl2), urea ((NH2)2CO) and thioacetamide (CH3CSNH2)
are used to synthesize ZnS for three hours. The grown ZnS nanostructures are then dried at room temperature.
Results showed that ZnS nanostructures were successfully grown onto Si(100) and Si(111) substrates. It was revealed
that substrate orientation controls the density of the nucleation of the ZnS nanostructures. It was found out that Si(100)
substrate have higher density of ZnS nanostructure as compared to Si(111) substrate. This might be due to the available kink
steps in the surface of Si(100) substrates that enhances the nucleation of ZnS nanostructures as shown in the figure below.
Furthermore, it was revealed that the band gap of the grown nanostructures is between 3.67 – 3.70 eV which is in good
agreement with the reported values.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
ZnS nanostructures
on Si(100) substrate
ZnS nanostructures
on Si(111) substrate
EFFICIENCY OF THE ESTIMATION OF THE HURST EXPONENT IN A
TIME SERIES BASED ON FREQUENCY DOMAIN BOOTSTRAPPING
Jonathan Madronero and Chita Evardone, Ph.D.*
Department of Mathematics, MSU – Iligan Institute of Technology
Tibanga Ave., Iligan City 9200
A time series is an ordered sequence of observations. As a sequence, getting a sample that captures the dependency
inherent in a time series poses a problem. Two approaches in time series analysis which are mathematically equivalent are
based on time domain and frequency domain. In this study, bootstrapping procedures from these two approaches namely, the
block, the periodogram and wavelet based bootstrapping, are reviewed and their performances are compared in the estimation
and investigation of the asymptotic properties of the Hurst Exponent, which is an approximate measure of the autocovariance
function which particularly measures the predictability of a time series. Validity of the estimate based on these resampling
techniques depends on the closeness of the generated resamples data to the time series data. Simulation methods, motivated
from the works of Dr. Alfonso Albano (Bryn Mawr College, 2011) reveal comparable performance among the three procedures.
As , the sample size and , the number of replications, increase the sampling distribution of the Hurst exponent approaches a
normal distribution and the Mean Squared Error (MSE) decreases, with the width of the approximate 95% Confidence
Interval, decreasing. But notably, block bootstrapping under the time domain exhibits better performance in terms of a smaller
MSE. Empirical investigation of the Hurst Exponent was performed on the Southern Oscillation Index Data (1876-2012).
ETHNOBOTANY OF SOME PILI (CANARIUM OVATUM) VARITIES NATIVE TO GUBAT, SORSOGON
Judith R. Chavez and Nina M. Cadiz*
University of the Philippines at Los Baños, College, Laguna
Pili (Canarium ovatum, Engl.) or Philippine nut has a potential as an export product. It is thriving commonly in the
Bicol region. Nonetheless, most researches on pili are largely from those collected in Albay. Thus, this study initiated to
document ethnobotanic informations about pili native to Gubat, Sorsogon.
Findings revealed that majority of the pili farmers in Gubat were males with pili farming experience ranging from
three to seventy (70) years, but palay and coconut farms were their main source of income. All of the farmers interviewed could
not tell the origin of the pili germplasm being inherent on their farms. Most farmers identified varieties B, D and E as their
personal choice and cultivate pili without employing fertilizers, pesticides, herbicides and mechanization on their farms.
Moreover, farmers just make use of the fruit particularly the seed to be processed as pasalubong/ gifts, as desserts on special
occasions, as source of planting material and/ or sold downtown. Though, a small number of farmers mentioned the loss of
some pili varieties due to tree cutting and destructive typhoons.
FABRICATION OF ALUMINUM NITRIDE/AIR-GAP DISTRIBUTED BRAGG REFLECTORS
FROM WET ETCHING OF SACRIFICIAL ALUMINUM LAYERS
Jonathan Ray R. Abat, Nemesio S. Mangila IV, Arnel A. Salvador, and Armando S. Somintac*
National Institute of Physics, University of the Philippines Diliman, 1101 Quezon City
Aluminum nitride/air-gap distributed Bragg reflectors were fabricated using selective wet etch to remove the
sacrificial aluminum layers. Aluminum nitride/ aluminum superlattice was prepared on a Si substrate via reactive radio
frequency (RF) magnetron sputtering. Scanning electron microscopy was also performed to determine the AlN and Al
thickness layers. The AlN/Al superlattice was annealed to improve the crystallinity of AlN layers and it was characterized
using x-ray diffraction. Crystalized AlN (002) was confirmed from the x-ray rocking curve. Finally, we performed reflectivity
measurements of the fabricated AlN/air-gap DBRs and it was recorded to be approximately 47% at the peak reflectivity.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
FLORISTIC DIVERSITY AND AVIFAUNAL COMPOSITION IN THE MANGROVE ECOSYSTEM
OF PRIETO DIAZ, SORSOGON, PHILIPPINES
Noel P. Labutap1, Rhia Odessa Gonzales- Geronimo*,
Juancho B. Balatibat* and Nelson M. Pampolina*
1
Graduate Student, MS Natural Resources Conservation – Coastal Resources Management
*
Guidance Committee, University of the Philippines Los Baños, College, Laguna 4031
E-mail: madlers_04@yahoo.com; nelsonmanguiatpampolina@yahoo.com
Mangrove ecosystem of Prieto Diaz, Sorsogon has been known for its ecotourism value aside from wealth of goods
and services it provides for human and coastal organisms. Nevertheless, this ecosystem is rapidly declining with time as a
result of destruction by natural calamities and various anthropogenic pressures existing therein. To compliment the need for
an up-to-date list of its mangrove flora and avifauna; and before possible local extinction of some threatened species in the area
occurs, this research was conducted. Through estimateS, 26 sampling plots with a dimension of 20m x 20m were established
for vegetation assessment. Regenerations within the plots were also captured from the systematically sampled 1m x 1m
subquadrats. Avifaunal species encountered within the 200- meter radius from the center of each plot were also identified.
Analyses of data were done using different available biodiversity software (Species diversity & richness by Henderson &
Seaby; EstimateS; and Binmatnest).
Result of the study enumerated a total of 42 mangrove species, 30 genera and 23 families; of which 28 species are
under the true mangroves category and the rest as mangrove associates. Included in the list is the Endangered Camptostemon
philippinense (Vidal) Becc. (Gapas-gapas), which shows no record from past surveys conducted in the area. Other important
mangrove species observed are the Near Threatened Aegiceras floridum Roem. & Schult (Tinduk-tindukan) and the Vulnerable
Ceriops decandra (Griff.) Ding Hou (Malatangal); and Avicennia rumphiana Hall f. (Miapi). Floristic diversity of the site both for the
canopy and regenerations resulted to a low diverse category with computed Shannon-Weiner diversity index value of 2.2490
and 2.3490 respectively. On the basis of species relative dominance, relative frequency and relative density; Avicennia rumphiana
Hall f. (Miapi), Avicennia marina (Forsk.) Vierh. (Bungalon) and Rhizophora stylosa Griff. (Bakauan bato) had the highest
computed value of 26.59%, 26.60% and 50.61% respectively. On the one hand, 30 bird species belonging to 9 orders and 24
families were identified; of which 2 species were considered threatened (Chinese Egret and Philippine Duck).
The results may have significant implications on the need for urgent attention in the aspect of mangrove conservation
and management, specifically dealing on species prioritization. However, continuous monitoring of the municipality’s
mangrove resources should also be initiated through time.
FNESTING TECHNIQUE USING REGIONAL CLIMATE MODEL VERSION 3
Robert B. Badrina, *Raquel dV. Francisco, PhD
Institute of Environmental Science and Meteorology, College of Science,
University of the Philippines Diliman, Quezon City, Philippines
The use of climate modelling is a way to represent numerically the complex climate system of earth.
Researchers are trying to utilize models using a set of mathematical equations to understand the processes going on and
to reduce the complexity of the climate system. In this study, FNesting technique was used in a Regional Climate Model
version 3 (RegCM3) to enhance the result of the model in comparison with the observed data. RegCM3 is a three
dimensional, hydrostatic, terrain-following sigma coordinate with horizontal resolution of 10 -100 km. FNesting is a one
way nesting option wherein the output of the first simulation which is in a lower resolution will be used as an input for
the second run having a higher resolution domain. The NCEP/NCAR reanalyze global data set was used as an input in
the model. We used 45 kilometer resolution for the first run which included parts of Southeast Asia and the second run
which is a 15 kilometer resolution centred in the Philippines. The result shows that the output of the one way nesting is
more comparable with the observed data compared to the output of the first simulation. This shows the potential used
of FNesting to improve the result of higher resolution since most of the impact models need to be in a local scale about
40 kilometers or less.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
GENE SILENCING OF VP9 GENE IMPAIRS WSSV INFECTIVITY ON Macrobrachium rosenbergii
Rod Russel R. Alentona, and Mary Beth B. Maningasabc*
a
Department of Natural Sciences, College of Nursing,
b
Department of Biological Sciences, College of Science, cMolecular Biology and Biotechnology Laboratory,Research Cluster for Natural and Applied
Sciences, University of Santo Tomas, España, 1015, Manila, Phillipines
White Spot Syndrome Virus (WSSV) remains the most widespread and devastating infectious agent that hit the
shrimp aquaculture industry worldwide. To date, there are no available remedies yet to combat WSSV infection. Hence,
functional studies on genes critical for viral infection, is essential in elucidating shrimp-virus interaction. Here we report, a
newly identified WSSV gene coding for VP9, a non-structural protein predicted to have possible involvement in viral
transcription. This study utilized gene knock-down technology through RNA Interference, to elucidate the function of VP9 in
shrimp-virus interaction. Three set-ups using Macrobrachium rosenbergii daqueti shrimps were prepared for treatment of VP9dsRNA, GFP-dsRNA, and PBS. Each shrimp was challenge with WSSV using 10-2 concentration, and survival rate was
recorded. The VP9- and GFP-dsRNA injected shrimps showed a significant survival rate of 80% and 100% respectively in
contrast to 20% of the PBS injected shrimps at 10 days post infection (Fig.1). Re-infection of shrimp survivors injected with
VP9- and GFP-dsRNA, using a higher viral titer at 10 -1 concentration, concurrent with the infection of new shrimp samples for
PBS control group, showed a significant survival rate (67% ) for VP9-dsRNA compared to that of GFP-dsRNA (0%) and PBS
(0%) on day 19 post-infection (Fig.2). Silencing of VP9 gene was observed as early as 0 dpi (Fig. 3), and a gene from other
region of the WSSV genome was also silenced as early as 1 dpi for the hemocyte samples (Fig.4). This further corroborates the
antiviral protection of VP9-dsRNA treatment in the challenge test data. Results showed that VP9, upon silencing, impairs
virus infectivity on its host.
Fig. 1 Silencing VP9 gene displayed a protective effect against WSSV.
VP9-dsRNA treated shrimps maintained a 60% survival, while PBS control
group reached 0% 29 days after infection.
Fig. 2Re-infection of shrimp survivors proved the anti-viral effect of
silencing VP9. VP9-dsRNA treatment (specific gene silencing) displayed a
sustained antiviral effect after re-infection with WSSV, as compared to
GFP-dsRNA (non-specific gene silencing).
WSSV clearance
Fig.3 RT-PCR showed VP9 gene was silenced 3 hours to 7 days postinfection (dpi). For VP9-dsRNA treated shrimps, for both tissues gills (g)
& hemocyte (h) VP9 gene was not expressed, and therefore silenced.
While EF-1α (internal control) was consistently expressed, indicating that
all other genes are still expressed except for the VP9 gene.
Fig.4 Silencing VP9 gene inhibited expression of other WSSV gene
ORF036. VP9-dsRNA treatment also silenced the expression of other
WSSV gene indicating viral clearance. Hence, the knock-down of VP9
also impairs WSSV infectivity.
GROWTH AND CHARACTERIZATION OF EMERALDINE SALT POLYANILINE (PANI) FILM ON
SILICON (N-SI) WAFER SUBSTRATE BY CHEMICAL POLYMERIZATION FOR DIODE APPLICATION
Patrick Alvin M. Alcantaraa, Bianca Rae B. Samboa and Reynaldo M. Vequizoa*
a
Materials Science Laboratory, Physics Department, MSU-Iligan Institute of Technology,
A. Bonifacio Avenue, 9200 Iligan City, Philippines
*Corresponding author: sirtrickz@gmail.com
The properties of the emeraldine salt form polyaniline (PANI-ES) films on n-type Si(100) wafer substrate is
presented. This growth of PANI-ES was done following the chemical oxidative polymerization of aniline with ammonium
peroxydisulfate as the oxidant and chlorine from hydrochloric acid as dopant. The PANI-ES on n-type Si(100) wafer were
characterized using Attenuated Total Reflectance (ATR)-FTIR spectroscopy, scanning electron microscopy (SEM) and
two-probe current-voltage electrical techniques to investigate PANI-ES growth, surface morphology and electrical properties
of the PANI-ES/n-Si(100).
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FTIR results showed unique absorption peaks indicating that PAni polymerizes on n-type Si (100) producing a
PAni-ES evident from similar peak intensity at 1568cm-1 and 1495cm-1 wavenumbers, respectively. Current-voltage
characterization further showed that the formed material has excellent electrical conduction as observed from its IV curve.
But due to the non-linearity of PAni-ES in its logarithmic plot it is believed to have various junctions formed between the
heterostructure in which different properties such as diode ideality factor and saturation current were calculated.
HEAVY METAL CADMIUM IN FEEDS AND TISSUES OF FEMALE DOMESTIC MALLARD
(Anas platyrhynchos domesticus L.) IN VICTORIA, LAGUNA AND CANDABA, PAMPANGA, PHILIPPINES
Josh Elisha R. Octura1, Renato S.A. Vega1*, Cesar C. Sevilla1, Ma. Stella C. Tirol2,
Severino S. Capitan1 and Angel Lambio1
1
MS Student, Associate Professor/Project Leader, Professor, respectively; Animal Breeding and Physiology Division, Animal and Dairy Sciences
Cluster, College of Agriculture, University of the Philippines Los Baños 4031, College, Laguna, Philippines;
Email: *rsavega@uplb.edu.ph or renevega10@yahoo.com; and
2
Associate Professor, College of Development Communication,
University of the Philippines Los Baños, 4031, College, Laguna, Philippines
This study was conducted to assess the cadmium residues in feeds and tissues and the physiologic condition of ducks in
Victoria, Laguna and Candaba, Pampanga. Ducks at peripubertal (4-8 months, n=43), mid (10-14 months, n=40) and late laying
(≥18 months, n=40) stages were sampled from different farmers in both locations. The commercial feed, snails, duck liver, ovary,
muscle and fat samples were analyzed for cadmium. The body condition score (BCS), bodyweight, and organ weights were
taken. Hepatosomatic, ovary-somatic and oviductosomatic indices were measured and plasma vitellogenin EIA was done using
bird vitellogenin antibody successfully. Results showed that maximum level of cadmium was detected in feeds (0.84 mg/kg)
and tissues (0.75 mg/kg) of ducks in both locations. No significant differences were observed in BCS, bodyweight and
organosomatic indices between ducks in different sites. Morphological abnormalities in the liver (Laguna=49%,
Pampanga=35.9%) and ovary (Laguna=13.6%, Pampanga=15.6%) were also comparable between ducks in both locations. This
study confirmed the presence of cadmium in feeds and tissues as well as the presence of abnormalities in the organs. Likewise,
in Candaba where Cd was higher, the plasma vitellogenin was lower (P<0.05), indicative of Cd effect on duck egg production.
IN SITU RUMEN DEGRADABILITY OF VARYING QUALITIES OF BASAL DIETS
WITH JACKFRUIT BY-PRODUCT AND SOYBEAN MEAL SUPPLEMENTATION IN CATTLE
Fatima A. Zamora and Lolito C. Bestil*
Department of Animal Science, Visayas State University, Visca, Baybay City, Leyte
Supplementation in ruminants improves voluntary intake and feed degradability. Two (2) trials were conducted to
evaluate the rumen degradation of various types of basal diet in cattle supplemented with jackfruit by-product meal (JBM)
alone, or in combination with soybean meal. The experiments tested four (4) types of basal diet such as fresh napier grass, corn
stover silage, rice straw, and sugarcane tops hay, incubated in situ in a ruminally-cannulated cattle for 24, 48, and 72 hours.
The experiments were laid out in completely randomized design (CRD) replicated four times. The results of the study with
jackfruit by-product supplementation alone showed significant differences in dry matter degradation (DMD) as well as
organic matter degradation (OMD), and rates of DM and OM degradation, in all treatments across incubation periods. Corn
stover silage revealed highest DMD and OMD values and rates with jackfruit by-product supplementation alone or in
combination with soybean meal. The addition of soybean meal into the jackfruit by-product supplement did not significantly
improve percent DM and OM degradability of basal diets showing that protein supplement is not very critical when jackfruit
by-product meal is already present in the diet. Nevertheless, the addition of protein source together with jackfruit by-product
meal showed beneficial effects for highly fibrous types of basal diet such as rice straw and sugarcane tops hay.
IN VITRO SCREENING OF THE VASORELAXANT ACTIVITY
OF Diospyros discolor Willd. (EBENACEAE) LEAF EXTRACTS
Renz K. G. Cadiang1, Marina O. Osi2, Jovencio G. Apostol1, Mafel C.Ysrael1,2,3
Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines, 2Faculty of the Graduate School,
University of Santo Tomas, Manila 1008, Philippines, 3Research Center for Natural and Applied Sciences,
University of Santo Tomas, Manila 1008, Philippines
1
Diospyros discolor Willd., an endemic Philippine tree, is used in traditional medicine for managing various ailments like
heart problems and hypertension. Because of its potential benefit to cause vascular smooth muscle relaxation, the present
study aimed to investigate the methanol, hexane, ethyl acetate, n-butanol and aqueous leaf extracts of D. discolor for their
vasorelaxant activity and total phenolic content. Vasorelaxant screening was carried out on isolated rat aortic rings. Using an
automated polygraph data acquisition system and chart recorder, acetylcholine was used to determine the integrity of
endothelium by relaxing the phenylephrine precontracted rings. Test rings were treated with the extracts and fractions in
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cumulative manner. Among the leaf extracts of D. discolor, Ethyl acetate fraction exhibited 52% relaxation at a concentration of
500 µg/ mL while methanol extract almost produced a 100% relaxation at the same concentration. Hexane and n-butanol
fractions exhibited 28% and 47% respectively at the highest concentration. Removal of the endothelium and incubation with
NG-nitro-L-arginine methylester (L-NAME) almost diminished the relaxant effects of the extract and its fractions. This study
indicated that the extracts possess endothelium-dependent and NO-mediated vasorelaxant effects. Folin-Ciocalteau method
also showed that all the extracts and fractions contained polyphenols expressed as gallic acid equivalent.
INVASION BIOLOGY OF SUCKERMOUTH ARMORED CATFISHES (PISCES: Pterygoplichthys)
Joel M. Chavez and Jose Santos R. Carandang VI*
Department of Biology, College of Science, De La Salle University
Suckermouth armoured catfishes (SACs) known locally as “janitor fish” have been introduced into Philippine waters.
SACs have been thriving in areas in the Laguna de Bay basin and are considered invasive by way of anecdotal accounts that the
fish is causing negative effects to humans and the environment. This study intends to shed light in the biology of this invasion
so appropriate plans to mitigate negative effects may be put in place. The results of the study reveal the following: 1) there are
two species of SACs in the Laguna de Bay area namely, Pterygoplichthys pardalis and Pterygoplichthys disjunctivus; 2) there is a
thriving SAC population that has equal sex ratio between males and females, females that mature at short lengths, females with
high fecundity, and a 10-month breeding season; 3) SACs are interacting with native species by being an opportunistic
detritivore, salinity tolerant, low oxygen tolerant, and protected by armor; 4) SACs are not a likely cause of biodiversity loss
more than the effects of human caused disturbance such as pollution but still a likelihood is probable; and 5) SACs are not
likely causes of habitat destruction and alteration but still the likelihood that they may cause such effects is probable. With the
cost of managing invasive species, the Philippines should prepare a plan for this invasive species that takes into account the
status of existing economic and social capacities and the status of environment of the invaded areas.
ISOLATION AND CHARACTERIZATION OF THE OIL BODIES AND OLEOSINS
FROM THE SEEDS OF MORINGA OLEIFERA LAM.
Abigail Loren T. Uy, Vivian A. Panes*
Ateneo de Manila University, Loyola Heights, Quezon City, Philippines, 1108
Oil bodies are plant organelles involved with mobilizing lipid reserves for germinative and post-germinative
processes (Huang 1996, Tzen and Huang, 1992). The protein oleosin is unique to oil bodies, it provides stability to the oil body
structure (Frandsen et al. 2001).
Extracted oil bodies from M. oleifera were subjected to morphometric analysis; pods of different stages of maturity
were compared in terms of yield. Bradford assay showed that pods stages 4, followed by 5, gave the best yield of oil bodies and
oleosins. Extracted oil bodies were viewed under light microscopy, sizes varied from around 0.5 to 10 um, with a few even
larger oil bodies at around 20 um.
Thin layer chromatography was performed on the neutral lipid component (which contains triacylglycerides) and
the phospholipid component separated from the oil bodies. Spots for TAG were observed for most runs. Tricine SDS PAGE
with urea showed 3 bands, sized 19, 24 and 33 kDa. This corresponds with IEF gel results, showing dark spots at 32 kDa (pH
4.7, 5.1, 5.2, 5.5, 5.6), 25 kDa (pH 6, 6.5) and 20 kDa (pH 3.1).
Isolated oleosins will also be purified by gel filtration chromatography, and will be sent for N terminal sequencing.
LANDMARK-BASED GEOMETRIC MORPHOMETRIC ANALYSIS ON BODYSHAPE
VARIATION OF Mesopristes cancellatus (Cuvier, 1829)
1
Christina A. Barazona, 1Mark Anthhony J. Torres-*, 2Jessie G. Gorospe*, 1Cesar G. Demayo1*
1
Mindanao State University- Iligan Institute of Technology, Iligan City LDN
2
Mindanao State University- Naawan Campus, Misamis Oriental
Phenotypic relatationship between male and female of protandic fish Mesopristes cancellatus suggest a minimal evident
variation between sexes which is within the range of the mean shapes. Males have higly fit shapes while females have greater
variation in body shapes particularly in the abdominal area. The descriminant analysis of the landmark points constructed
results to 85.54% classification and 68.79% explained variance from warp analysis which is associated with the body depths
and the length of the abdomen, the position of the eye and the distance of the eye to the pectoral fins. Major shifts of the shape
transformation are projected longitudinally with relative constrictions towards the anterior portion of the body. The
morphometric references show a linear relationship and direct proportionality ralated to the length of samples.
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--MAGNETIC FIELD DEPENDENCE OF THE TRANSITION WIDTH
OF Bi2Sr2-xMnxCaCu2Oy SUPERCONDUCTING FILMS
Leonalyn V. Bambao and Roland V. Sarmago*
Condensed Matter Physics Laboratory,National Institute of Physics, University of the Philippines Diliman,Quezon City, 1101
Corresponding author: leonalyn.bambao@gmail.com
We investigate the effect of magnetic field up to 1T on Mn doped Bi 2Sr2-xMnxCaCu2Oy superconducting films. Films
deposited via the sedimentation deposition method and annealed at 850°C for 24 hours all exhibited the usual metallic
behaviour at normal state with almost the same onset transition temperature. However, the zero resistance transition appeared
to be increasing with increased Mn content up to x=0.02 and then decreasing at higher doping concentration. Results point to
increased defects introduced by the Mn dopant to be the primary factor on the change of the transition width. This study gives
more understanding to the effect of introducing a magnetic element to a superconductor.
MECHANIZED DRY DIRECT SEEDED RICE SOCIAL ENTERPRISE UPHOLDING FARMER FAIR TRADE
Lourd Franz M. Gabunada
University of the Philippines Los Baños, 4031, College, Laguna, Philippines
Hult prize is the world’s largest student movements for social good. Together with the Clinton Foundation, the
HULT International Business School invests $1,000,000 USD every year against the social enterprise which comes up with the
best business solution that targets eradicating global poverty.
This year’s challenge is to address global food security, and our team came up with a social enterprise highlighting a
rice crop intensification scheme by Mechanized Dry Direct Seeding (MDDS) - a promising method primarily due to simplicity
and the need for less labor. Moreover MDDS comes with environmental benefits as it requires less water and causes less
methane emissions along with other notable benefits of direct seeding which include earlier seedling development. The
proposed social enterprise also aims to facilitate the intermediation of the rice supply chain so as to promote famer fair trade.
The notion of this social enterprise is of utmost significance as rice is almost equated to food security for the reason that it is
staple to more than half the world’s population. Thus, increasing rice production is crucial, given the estimate that world food
production will have to double by the next couple of decades to guarantee food security. The bulk of increase will have to be
achieved through intensification of production, as expansion of cultivated areas is reaching sustainable limits as well as being
greatly affected by the expansion of urban areas.
The proposed social enterprise scheme arises in line with the pursuit to address the dismal trend of urbanization
consequentially resulting to the conversion of agricultural areas for urban/suburban development. Nowadays more and more
people are less attracted to agricultural investments. Rice farming in particular has become less popular to farmers as it
requires intensive labor. This is further exacerbated by the existing rice supply chain wherein unlike any other Asian
countries, the Philippines suffers from low production and a small number of rice post harvest facilities (mills and cold storage
facilities). Low production is also the reason for the higher cost of transporting goods. Thus, by facilitating the improvement
of the midstream market, we can help in developing the wholesale market and strengthen smallholder farmers and medium
enterprises that employ many people in the countryside. In parallel, we can create a wave of change which can reverse the ill
effects of urbanization by encouraging more people (even smallholder farmers) to invest in rice production once again.
MINERAL RESOURCES IN THE PHILIPPINES: NICKEL LATERITES
Russel M. Ong, and Carlo A. Arcilla*
University of the Philippines – Diliman, Quezon City
Lately, new technology have been developed that allows us to study the physico-chemical, mineralogical, and
structural properties of various earth materials like soil, rock, air and water. The use of X-ray and spectroscopic instruments
have revolutionize our understanding of mineral resources which before are exploited for human use with less importance
given to the study on their formation and implications. However, a deeper study of these materials leads to a better
understanding of the processes shaping the earth for millions of years. Our study on nickel laterites consists of field
observation, analytical characterization, and data correlation of the different Nickel rich soil deposits derived from mantle
rocks which are abundantly distributed all over the Philippines. Ni rich soil originally derived from metal rich rocks that are
chemically weathered form regoliths have a high concentration of Ni. Currently, 70% of the total world nickel resources are
found in laterites, and 17% of resources are found in the Philippines. Instruments such as X-ray diffraction (XRD); X-ray
fluorescence (XRF); Fourier transform infrared spectroscopy (FT-IR), Inductively Mass Spectroscopy (ICP); petrographic and
scanning electron microscope, energy dispersive spectrometry, provide us the information on formation of crystal phases as
well as the mobility and bioavailability of metallic elements during the weathering of the host rocks. Mineralogy and
geochemistry of laterite has a wide implication on metallurgy, metallogeny, environmental remediation, natural analogues, and
resource estimation.
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NORM-PRESERVING INVERSE BEST APPROXIMATION IN HILBERT SPACES
Rosalio G. Artes, Jr.* and Karlo S. Orge
Department of Mathematics, College of Science and Mathematics,
MSU-Iligan Institute of Technology, Iligan City
Let be
a sequence of pairwise disjoint closed convex subsets of a Hilbert space
paper, we investigated the properties and established some results on the solution
of the linear system
where
is the orthogonal projection of
onto
, satisfying
can always find an approximate solution for (1) in
such that
for every
for every
and
. In this
. Moreover, since we
, we investigated the properties of the approximate solution
where
is of the form
with
and
.
OCCURRENCE OF FUNGAL FOLIAR DISEASES ON SELECTED MANGROVE SPECIES IN OIL-SPILLED AREAS
IN TAKLONG ISLAND NATIONAL MARINE RESERVE, NUEVA VALENCIA, GUIMARAS, PHILIPPINES
Diana Dalino Panaguiton and Resurreccion B. Sadaba*
Division of Biological Sciences, College of Arts and Sciences
University of the Philippines Visayas, Miagao, Iloilo
*Corresponding author: sadaba09@yahoo.com
The Solar 1 oil spill off the coast of southern Guimaras in 2006 affected the mangroves within Taklong Island and
Tandog Island National Marine Reserve (TINMR) in Nueva Valencia. This study aimed to determine the severity and
incidence of fungal foliar diseases as an indicator of weakened state on selected mangrove species. Specifically, it aimed to 1).
Isolate, characterize and identify fungal species associated with foliar diseases of selected mangrove species; 2). quantify the
occurrence of fungi and 3). evaluate disease incidence and severity on selected mangrove hosts. A total of 500 symptomatic
leaves were randomly sampled from six oil-contaminated sites and one un-contaminated site within the reserve in March 2012.
Fungi were isolated, described and identified to the genus level only based on available keys and monographs. In general,
disease incidence at oiled sites (68-79%) were significantly higher compared with the un-oiled site (54%) but severity was not
high. All species showed higher incidence at oiled sites compared with un-oiled sites. A total of 28 species were collected of
which only Hyphomycte 2 was the most frequently collected species at 10.8%. Species consistently isolated from foliar lesions
were Pestalotia sp., Spermatia, Fusicoccum sp., Phyllosticta sp., Hyphomycete 2 and Ascomycete 1. Results of this study showed that
despite the widespread occurrence of fungal foliar disease among the oil-contaminated mangroves, severity was low. This
might be attributed to the improved resistance of the trees indicative of the improvement of overall health of mangroves
towards recovery six years after the spill.
PATHOGENIC YEAST STERILIZATION VIA MICROWAVE ATMOSPHERIC PEN PLASMA (APP)
Joey Kim T. Soriano1**, Leo Mendel D. Rosario1,2, Ma. Camille C. Lacdan1,
1
Henry V. Lee Jr. , Julie Anne S. Ting1, Henry J. Ramos1*, Maria Auxilia T. Siringan3* and Roy B. Tumlos1,4*
1
Plasma Physics Laboratory, National Institute of Physics, University of the Philippines Diliman, Diliman, Quezon City 1101, Philippines
2
Sciences Department, College of Arts, Sciences, and Education, FEATI University, Helios St., Sta. Cruz, Manila 1003, Philippines
3
Microbiological Research and Services Laboratory, Natural Science Research Institute,
University of the Philippines Diliman, Quezon City 1101 Philippines
4
Department of Physical Sciences and Mathematics, College of Arts and Sciences,
University of the Philippines Manila, Taft Avenue, Manila 1000, Philippines
**Corresponding author:jsoriano@nip.upd.edu.ph
This study tests the sterilization feasibility of the microwave atmospheric pen plasma (APP) on yeast infected
polypropylene (PP) tubes. Candida utilis and Candida tropicalis suspensions inoculated on PP tubes are exposed to microwave
APP using Ar/N2 gas and 250 W absorbed microwave power at various exposure time. Sterilization using the microwave APP
is feasible and effective in reducing significant number of C. utilis and C. tropicalis colonies as revealed by microbial analysis using
Decimal reduction time value (D-value). Images of the C. utilis cells before and after plasma sterilization are observed in the
Scanning Electron Microscope (SEM).
Figure 1. SEM images of C. utilis (a) before and
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PERFORMANCE AND MEAT QUALITIES OF BROILER FED DIETS
WITH DIFFERENT LEVELS OF JACKFRUIT (Artocarpus heterophyllus, L.) SEED MEAL
Ma. Rosa De Lima C. Gerebise and Alberto A. Taveros*
Visayas State University, Visca, Baybay City, Leyte
A study was conducted to assess the feeding value of Jackfruit (Artocarpus heterophyllus, L.) seed meal (JSM) and the
effect on growth performance, dressing percentage, sensory evaluation, metabolized nutrients and cost and return benefits of
broiler husbandry at 35 days growing period. The effect of treatments were investigated in a 240 Cobb broilers grouped into
four dietary treatments (control, 5% JSM, 10% JSM, and 15% JSM diets) at 10 birds per 6 replicates following Completely
Randomized Design (CRD).
No significant differences (p>0.05) were observed between the four treatments in live weight and average daily gain;
however T1 with 5% jackfruit seed meal had the highest live weight and average daily gain and dressing percentage as well as
income, profit, RAFACC and ROI in broiler production. Furthermore, incorporation of 5% jackfruit seed meal increased
dressing percentage of broilers. Generally, the acceptability level for flavour, juiciness and tenderness are comparable for all
treatment diets however highest mean general acceptability was on T1 (5% JSM) described as like moderately to like very
much, while carcass acceptability on color favored for T0 (control) thus significantly different (p<0.05) from T1 (5% JSM).
Moreover, higher percent in T0 (control) was observed in metabolized energy though no significant difference (p>0.05) in
metabolized protein assimilation jackfruit seed meal.
PERFORMANCE OF GROWING LAMBS AS INFLUENCED BY LIQUID ACID WHEY SUPPLEMENTATION
Angie R. Poliquit and Serena L. Sanchez*
Department of Animal Science, Visayas State University, Visca, Baybay City, Leyte
Twelve male growing lambs were used in a randomized complete block design to determine the effects of feeding
liquid acid whey (LAW) on the performance of the animal, its dry matter intake, nutrient digestibility, weight gain and
ruminal pH. Animals received a diet consisting of Napier grass (Pennisetum purpureum, Schumacher). Treatments were Napier
grass plus concentrate, Napier grass plus LAW and Napier grass plus concentrate plus LAW. Individual Dry Matter Intake
(DMI), biweekly Weight Gain (WG) and ruminal pH were measured. Diet digestibility was determined using the total
collection method. Results showed that LAW increased (P<0.05) WG and diet digestibility (dry matter, organic matter and
crude protein). Liquid acid whey had an average digestibility of 54.41% for dry matter, 83.94% for organic matter and 87.56%
for crude protein. Ruminal pH two hours after feeding was stable for animals with LAW alone. However, DMI was similar
(P>0.05) among treatments. It can be concluded that including LAW in the diet significantly improved WG, diet digestibility
and ruminal pH without affecting DMI.
PHENOTYPIC AND GENETIC CHARACTERIZATION OF HALF-SIB PROGENIES OF THREE EUCALYPTUS
(Eucalyptus deglupta x E. pellita) HYBRIDS IN NORTHERN MINDANAO, PHILIPPINES
Albert A. Piñon1, Wilfredo M. Carandang2*, Edwino S. Fernando2, Cesar C.Nuevo3,
Manuel L. Castillo2, Marco A. Galang2, and Marilyn O. Quimado2
1
MS Student, CFNR, University of the Philippines Los Baños, Laguna, Philippines
2
Professor, CFNR, University of the Philippines Los Baños, Laguna, Philippines
3
Consultant, Claveria Tree Nursery, Inc., Claveria, Misamis Oriental, Philippines
Combined phenotypic and genetic characterization studies in the Philippines are limited. An initial attempt was
conducted by combining these two strategies in the context of forest genetic improvement. Using three F1 hybrid parents and
seventy-four surviving F2 half-sib progenies of E. deglupta x E. pellita hybrids, the economically important traits were
characterized, while the genetic variants were identified using Random Amplified Polymorphic DNA (RAPD) analysis.
Outcome of the analysis of variance revealed that the three F2 half-sib progenies performs poorly due to the occurrence of
inbreeding depression (ID), as reflected in a non-significant variation within and among the families in all phenotypic traits
used, except in Total Height and Volume. Correlations between phenotypic traits indicated that future selection using either
of the traits studied, particularly those with the highest value except for PS, can result to better volume harvest. Conversely,
RAPD analysis for F1 and F2 generations using fifteen primers proved that F1 hybrids were relatives and ID indeed, reduced the
performance of the F2 generation. Phenotypic and genetic correlations emphasized that the hybrid family with the highest
number of detected bands and polymorphic loci, percent polymorphism and gene diversity index has a greater chance of
surviving in the progeny trial. Ranking analysis between F2 progenies determined the superiority of EDP1 F2 when it comes to
phenotypic performance, while EDP2F2 outperformed the other populations genetically. Overall, EDP1F2 showed superior
performance.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
POPULATION DYNAMICS AND DIVERSITY OF INSECTS IN PUMMELO GROWN
UNDER ORGANIC AND CONVENTIONAL PEST MANAGEMENT SYSTEMS
Larry V. Aceres1, M. J. C. Ceniza*, L. M. Noriel*, E. A. Vasquez* and Z. C. Gonzaga*
1
University of Southeastern Philippines Tagum-Mabini Campus, Apokon, Tagum City,
* Visayas State University, Visca, Baybay City, Leyte
Population dynamics and diversity of insects were recorded and measured using ecological indices (diversity, evenness, and
dominance and similarity indices) for 12 months in pummelo grown under organic and conventional pest management systems. Four
sampling techniques were used in this study: Malaise trap, yellow pan trap, light trap and direct visual count from three pummelo
orchards in Davao Province. Characteristics of the pummelo orchards are as follows: site 1 is a pesticide-free orchard; site 2 is a
“diversified organic” pummelo orchard and; site 3 is a commercial pummelo orchard.
The Citrus Rind Borer (CRB), the most serious pest of pummelo was recorded to attack pummelo all year round with
population peaks in the months of January and April. This was found to be correlated to the presence of preferred stages of pummelo
(flowers, and small fruits) but not to weather factors (temperature, relative humidity and rainfall). Pest population was very high in
pesticide-free orchard while manageable population was recorded in diversified organic and commercial pummelo orchards.
Other pests such as the citrus thrips and purple scale, which are considered as minor pests in pummelo were very high in
commercial pummelo orchard while negligible number of these pests was recorded in pesticide-free and diversified organic pummelo
orchards. In contrast, a very high population and diversity of parasitoids and predators were documented in both pummelo orchards
whereas very low populations and less diverse natural enemies were recorded in commercial pummelo orchard.
Brachymeria sp., a pupal CRB parasitoid was present throughout the year with high percent parasitization recorded for
pesticide-free and diversified organic pummelo orchards while zero parasitization and/or population in commercial pummelo orchard.
Moreover, higher insect faunal family diversity and evenness with low dominance indices in pesticide-free and diversified
organic pummelo orchards than in the commercial pummelo orchard. Furthermore, the pesticide-free and diversified pummelo
orchards obtained very high similarity indices indicating the same arthropod family compositions between the two sites.
RELATIVE WARP AND CORRELATION ANALYSIS BASED ON DISTANCES OF THE MORPHOLOGICAL SHELL
SHAPE PATTERNS OF Pomacea canaliculata Lamarck FROM JAPAN AND THE PHILIPPINES
1
Carlo Stephen O. Moneva, 1Mark Anthony J. Torres*, 2Takashi Wada, 3Ravindra Joshi, 1Cesar G. Demayo*
1
Department of Biological Sciences, College of Science and Mathematics; MSU-Iligan Institute of Technology, Andres Bonifacio Avenue, Tibanga,
Iligan City, Lanao del Norte, Philippines; 2 Kyushu-Okinawa National Agricultural Research Center, Koshi, Kumamoto, Japan; 3
College of Agriculture, Forestry and Fisheries, Fiji National University, Koronivia Republic of Fiji
The Golden Apple Snail (GAS), Pomacea canaliculata Lamarck is considered one of the serious agricultural pests of rice
in Asia. It is being argued that rapid invasion of this species in many variable habitats suggests genetic variability and
differentiation which could be expressed at the level of the phenotype. It is therefore the major objective of the study to explore
possible phenotypic differentiation in the organism especially in the shape of the shell using geometric morphometric (GM)
analysis. Specifically, this study aimed to determine conchological variation in populations of GAS in the Philippines and
Japan. Three aspects of the shell shape were studied, which includes the ventral/aperture, dorsal and the top/whorl portion of
the shell using correlation analysis based on distances (CORIANDIS). CORIANDIS was used in order to visualize congruence
of multivariate traits among P. canaliculata populations. The results showed that P. canaliculata shell varies in shape and
variability may signify distinctive genotypes or adaptation to varying environments exhibited by Japan and the Philippines.
SCREENING OF ACTINOMYCETE ISOLATES FOR PLANT GROWTH-PROMOTING ACTIVITIES (GPA)
1
Jayvee A. Cruz and 2Erlinda S. Paterno, Ph.D.*
1
Philippine Rice Research Institute, Muñoz, Nueva Ecija
2
Professor Emeritus, ASC, UPLB, College, Laguna
At present, there is a low production of upland rice in the Philippines which is approximately 2 t/ha. This study examined
the effectiveness of actinomycetes to enhance the growth and yield of upland rice. Actinomycete isolates were screened for IAA
production, ACC deaminase production, nitrogen fixation, phosphate solubilization, and siderophore production in vitro. Based on the
screening results, five isolates were selected. Response to inoculation was evaluated in the growthroom and screenhouse using upland
rice as the test crop.
In a growthroom experiment, the population of actinomycete in the rice rhizosphere (R) was compared with that in the
non-rhizosphere soil (S). The high R: S ratio by Isolate 5 suggested better root colonization. In a screenhouse experiment, full rate
fertilization in combination with Isolate 5 increased percent filled grain by 20%. On the other hand, half rate fertilization in
combination with Isolate 5 increased percent filled grain by 16%. Similarly, half rate fertilization in combination with Isolate 3 or
Isolate 4, Isolate 1, and Isolate 2 increased percent filled grain by 15%, 12%, and 10%, respectively.
Interaction effect of rates of fertilization and actinomycete inoculation significantly increased P uptake of shoots and roots
by 136% and 32%, respectively.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
SEASONAL VARIABILITY OF MEIOFAUNAL COMMUNITY IN A SEAGRASS BED
IN SOUTHERN GUIMARAS, PHILIPPINES
Lorelie A. Burgos and Annabelle del Norte-Campos*
Marine Bio Lab, Division of Biological Sciences, CAS, Up Visayas, Miagao Iloilo
E-mail: lenathena3@yahoo.com; Telefax: + 63 33 315 9271
Seasonality of the meiofaunal assemblage in a seagrass bed was investigated. The study was conducted in Calaparan
seagrass bed located in the northeastern portion of the Taklong Island National Marine Reserve (TINMAR) (10°24’17’’ N and
122°30’33” E) in Southern Guimaras, Central Philippines. The area is dominated by Thalassia hemprichii. Sampling was done in
May and August 2011 and in February 2012.
Meiofaunal density ranged from 144.3 to 283.4 individuals ● cm-2 with a mean density of 677.1 individuals ● cm-2.
Highest densities were recorded in August 2011, during the southwest monsoon, and lowest in February 2012, during the
northeast monsoon. A total of forty two taxa were recorded. The assemblage was dominated by nematodes (42.7%) and
harpacticoids (24.5%), followed by polychaetes (22.3%). Nematodes were most abundant in May while harpacticoids showed
highest densities in August. High density in August might be attributed to nutritional inputs due to run-off.
SEXUAL DIMORPHISM IN MODULARITY AND INTEGRATION IN THE FORE AND HIND WING
OF THE COMMON EVENING BROWN BUTTERFLY MELANITIS LEDA LINNAEUS 1758
(Nymphalidae: Lepidoptera)
¹Vanessa Mae C. Tumang*, ¹Mark Anthony J. Torres* and ¹Cesar G. Demayo*
1
Department of Biological Sciences, College of Science and Mathematics; MSU-Iligan Institute of Technology,
Andres Bonifacio Avenue, Tibanga, Iligan City, Lanao del Norte, Philippines
It has been proposed that the specular diversification in butterfly wing patterns has been possible owing to the
evolutionary and developmental flexibility that arises from their organization in different series or types and from their
distribution in individual wing regions. This organization of modularity and integration often results to covariation among
various parts in organisms. In this study, the patterns of modularity and integration of the fore and hind wings in Melanitis leda
was determined by analyse of five hypothetical developmental modules using the MINT software (Modularity and Integration
Tool, ver. 1.5). A total of 195 points were used to trace and outline the margins of the wings as well as the major veins. The test
was conducted based on a Wishart/Monte Carlo test on a total of 1,000 replicates. The best fit models were determined with
the highest P-value and lowest gamma value. Different pattern of developmental modules were found. This was caused by
effects associated with the environment, whether phenotypic responses (plasticity) or the signaling sources for position and
morphology of pattern elements only covers short distances. Results also suggest that the compartments are autonomous unit
of gene regulation and the major veins served as boundaries.
SIMULATION STUDY OF THE FILTERED BACK PROJECTION METHOD
FOR PROTON COMPUTED TOMOGRAPHY OF BONE TISSUE
Ronn Marr M. Perez and Salasa A. Nawang*
Computational Physics Laboratory, Physics Department, MSU - Iligan Institute of Technology,
A. Bonifacio Avenue, Tibanga, 9200 Iligan City, Philippines
Computed Tomography (CT) imaging technique is used for accurate determination of the tumor locations. Instead
of using the conventional x-ray CT (xCT), proton CT (pCT) technique is adopted because of its low entrance dose, lessening
the radiation dose to healthy tissues. The fast computing Filtered Back Projection (FBP) method is used for the image
reconstruction and analysis of pCT.
Filtered Back Projection method was used in xCT reconstruction utilizing the intensity of the transmitted photons
over a number of projections. In implementing FBP to pCT, the path of proton as it traverse the body was assumed to be
straight by excluding proton data with large deflections compared to its entrance path. By utilizing proton energy loss
measurements over a number of projections, an FBP reconstructed image is acquired.
In the simulation setup, a 5cm x 9cm x 10cm elliptical tube bone phantom inside a 10cm x 10cm x 10cm water
phantom is used. 1200 proton rays where fired for every projection at one-degree increments for a total of 360 projections.
Results have shown that an image of the cylindrical bone inside a water phantom is produced.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
SITUATION ANALYSIS OF SOLID WATE MANAGEMENT AT A LOCAL HIGH SCHOOL IN REGION IV:
A BENCHMARK FOR ESTABLISHING SOLID WASTE MANAGEMENT PLANS
IN PUBLIC HIGH SCHOOLS IN THE SOUTHERN TAGALOG REGION
Daryl C. Pasion1 and Dennis S. Erasga, PhD2
1
Biology Department, De La Salle University
2
Behavioral Sciences Department, De La Salle University
This study is a situation analysis of solid waste management of the Quezon National High School (QNHS) in Lucena
City. It aims to launch a benchmark for establishing a solid waste management plans in public high schools in the Southern
Tagalog Region. Its research variables include among others the necessary policy, manpower, equipment, facilities, and
resources. The study also sought to examine the pattern of respondents’ belief about waste management and its effect on their
attitude and behaviors on waste management. A situational audit and a survey were the main tools of the study. In-depth
interviews with students and school officials were conducted. Findings suggest that despite being the largest and most
populated public high school in the Southern Tagalog Region, QNHS neither have a solid waste management system nor any
formal-written SWM Plan; no Materials Recovery Facility (MRF), and relies solely on the open dumpsite operated by the city
government. Waste collection depends almost wholly on its five-man buildings and grounds maintenance team. Overall, the
study indicated stronger intention to engage in a waste management behavior by the respondents. The findings suggested that
there is a need to improve the current solid waste management practices in QNHS which include behavioral and attitudinal
changes vis-à-vis solid waste reduction, reuse and recycling for sustainable environmental management. The paper ends with a
template of Solid Wastes Management System Plan appropriate for QNHS campus.
STRUCTURAL AND SUPERCONDUCTING EFFECT OF DOPING MANGANESE
ON STRONTIUM-SITE OF Bi2Sr-2CaCu2O8+δ
Alvin Carl M. Santiago and Roland V. Sarmago*
Superconductors Group, Condensed Matter Physics Laboratory, National Institute of Physics,
University of the Philippines Diliman, Quezon City, 1101
Corresponding author: asantiago@nip.upd.edu.ph
We report the effects of doping Manganese (Mn) on phase evolution and superconducting properties of Bi 2Sr2CaCu2O8+δ. X-ray diffraction shows that at low Mn doping levels, Mn is successfully incorporated in the Sr-site of Bi-2212.
However at higher concentration secondary phases start to appear indicating impurities related to Mn compounds. The critical
temperature increases with Mn doping however further addition results into decreasing critical temperature. Also a second
transition temperature appear at higher concentration (x=0.3) and further addition results into non-superconducting samples.
THE FLORY INDEX OF FRACTIONAL BROWNIAN POLYMER USING MONTE CARLO SIMULATION
Roel N. Baybayona, Cresente O. Cabahug*, Sim P. Bantayan*, Jinky B. Bornales*, Beverly B. Gemao*, Dennis A. Arogancia*
a*
Physics Department, Mindanao State University-Iligan Institute of Technology,
Bonifacio Avenue, Tibanga, 9200 Iligan City, Philippines
One can predict the size of a polymer by finding its end to end distance, R. Theoretically, P. Flory found out that it
is proportional to the number of monomer N with some scaling exponent , i.e.
where
is the Flory index in d
dimension. Besold et. al [1] used Edwards model [2] and the concept of Brownian motion to compute the Flory index by
simulations. In this paper, we want to extend the idea using the concept of fractional Brownian motion which is capable of
long range monomer interaction.
Fractional Brownian motion is one generalization of Brownian motion. In this concept, the Flory index can be
theoretically generalized into
Monte Carlo simulation.
where H is the Hurst parameter as mentioned in [3]. We have tested its validity using
In the simulation, we use the code of Samuel and Bock [to be published] considering d=1, 60≤N≤240 and H∊[0.1,0.5].
Our results in comparison with the theoretical prediction are shown in Figure 1 and it is evident that our simulated values are
in good agreement with the theory as proposed in [3].
Figure 1. Comparison between simulated and
theoretical values of Flory index.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
USING GIS AS TOOL TOWARDS A CLIMATE-DISASTER RESILIENT COMMUNITY:
FLOOD RISK MAPPING IN MARIKINA CITY, PHILIPPINES
Teodorico L. Marquez Jr. and Perlyn M. Pulhin
School of Environmental Science and Management, UPLB, College, Laguna
E-mail: ppulhin@yahoo.com , foresterchodox@gmail.com
The Philippines ranks as the third most disaster-prone country in the world because of its high exposure to natural
calamities.Over the next few decades, the increasing frequency of climate-related disasters such as typhoons, cyclones and
floods and the extent of damage and loss is likely to continue, if not accelerate due to climate change. Among the disasters
occurring in the country, flooding is the most common being a direct consequence of an average of 20 typhoons that enter the
Philippine Area of Responsibility. The levels of flood risk, particularly in cities have been rising due to rapid population growth
and urbanization.
Deadly floods that have inundated almost all of the Philippine capital are less a natural disaster and more the result of
poor planning and governance, slack enforcement and political self-interest. Recent extreme flood events have had devastating
socio-economic effects and threatened the development prospects. This study presents a tool that can be enhanced for more
effective local development planning and governance through a systematic approach of preparing hazard, vulnerability and
flood risk maps of Marikina City using Geographic Information System. If the local government units (LGUs) can be
empowered by developing their capacity to use such as tool, communities will be more prepared and resilient in the face of
disasters and changing climate.
The generated maps in the study were able to determine the coverage of the areas in Marikina per barangay exposed
to various levels of flood hazards. Designation of weights for each flood hazard factors was done using Analytical Hierarchical
Method. Areas vulnerable to flooding were also estimated per barangay and Rank Sum Method was used to obtain weights.
The coverage of the areas in the city per barangay exposed to various levels of flood risk was determined through raster
analysis. Overlaying and raster calculation of the flood hazard and flood vulnerability maps produced the flood risk map.
If the results of this study can be validated on the ground, it can help in strengthening the disaster risk reduction and
management plan of Marikina City. A well-informed community can take better precautionary measures and therefore increase
their adaptive capacity in the event of disasters. The national climate change adaptation planscan be localized to incorporate
site-specific flood risk reduction measures. Results of which could assist the LGUs at the municipal and barangay level in
conducting thorough assessment towards a more resilient community. Maps that were generated in this study could serve as
supplemental guide in improving the programs on relocation, emergency preparedness, river rehabilitation, solid waste
management and other developmental projects.
VOMIFOLIOL, A MEGASTIGMANE SESQUITERPENE
FROM THE LEAVES OF Psychotria gitingensis ELMER
Jameson A. Eusebio1, Grecebio Jonathan D. Alejandro1,2, and Mario A. Tan*1,2
The Graduate School1 and Research Center for the Natural and Applied Sciences2,
University of Santo Tomas, España, Manila, 1015, Philippines
Phytochemical studies involving the genus Psychotria (Rubiaceae) have been conducted and it has been established
that many species under this genus contain interesting chemical constituents. This research intended to isolate and identify
the secondary metabolites from the crude leaf extract of Psychotriagitingensis Elmer, a plant species endemic to the Philippines.
The crude methanolic extract was subjected to acid-base partitioning which gave the crude base extract. Initial gravity column
chromatography of the crude base extract afforded nine major fractions (PgC-A to PgC-I) and PgC-F and PgC-G gave light
orange spots in TLC using Dragendorff’s reagent, which may be indicative of the presence of alkaloids. Further purification of
both PgC-F and PgC-G led to the isolation of vomifoliol, a megastigmanesesquiterpene whose structure was elucidated based
on extensive spectral analyses (1D and 2D NMR, and MS) and comparison with reported literature values. Vomifoliol showed
moderate activity against Klebsiellaoxytoca using the agar diffusion paper-disc method. This study represents the first
phytochemical work on Philippine Psychotria, particularly on P. gitingensis, and the first isolation of vomifoliol from the genus
Psychotria.
* Adviser
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
YIELD AND NUTRIENT UPTAKE OF LOWLAND RICE AS AFFECTED BY APPLICATION
OF CHICKEN MANURE UNDER DIFFERENT WATER MANAGEMENT SYSTEMS
Melanie D. Ratilla and Alfredo B. Escasinas*
Agronomy Division, Department of Agronomy and Soil Science, Visayas State University, Baybay City, Leyte
Application of fertilizer is one of the cultural practices that influence crop productivity. This study was conducted to
evaluate the nutrient uptake of rice plant fertilized with commercial chicken manure under different water management
systems, determine the appropriate water management for optimum yield of lowland rice, and evaluate the profitability of
growing lowland rice fertilized with commercial chicken manure under different water management systems.
An area of 667 m2 was laid out in split plot in randomized complete block design (RCBD) with 3 replications with
water management (WM1-Flooded; WM2-Unflooded) as the main plot and rate of fertilizer application (T 0-Control,
T1-Inorganic fertilizer (90-60-60), T2-Chicken manure at 4.5 t/ha, T3-Chicken manure at 9.0 t/ha) as the subplots. Replications
and treatment plots were separated by 1.0 m alleyways. Individual plots size was 5m x 4m. Each parameters was analyzed
using split plot design while mean comparison was done using Honestly Significant Difference (HSD) or Tukeys’ test.
Among the rice yield parameters gathered only the grain yield showed significant difference. Grain yield of rice plants
applied with chicken manure was numerically higher than the control and was comparable to those applied with 90-60-60 kg
N P2O5, K2O/ha inorganic fertilizer. This implies that increment in grain yield of rice could still be realized even if only chicken
manure is applied. Increasing its application from 4.5 to 9.0 t/ha did not give remarkable results in the production of grains.
This result could also be due to the beneficial effects of applying chicken manure which might have improved the physical
condition of the soil. As expected, the inorganic fertilizer treatments yielded higher than the untreated control. A significant
interaction between water management and fertilizer application was noted on grain yield. Application of chicken manure at
9.0 t/ha resulted in the grain yield of 2.78 t/ha in unflooded condition which was statistically similar to the yield under flooded
condition of 2.83 t/ha. These yield levels did not differ significantly with the yield from inorganic fertilizer application
(3.14 t/ha) in unflooded condition and with the yield of rice under flooded condition applied with 4.5 t/ha chicken manure.
The results of this experiment indicate that lowland rice can be productively grown under unflooded water
management system utilizing chicken manure as organic fertilizer. The culture of rice under unflooded condition can save
water by more than 50%. Growing lowland rice under flooded condition is more profitable than unflooded condition under
the condition of this experiment. The application of chicken manure resulted in the higher total nutrient uptake of lowland
rice. The nutrient uptake for nitrogen, phosphorus and potassium were higher in flooded than in unflooded water management
system. The use of chicken manure as organic fertilizer is recommended for conversion from conventional to organic rice
production.
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2013 | 2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
2nd National DOST-SEI ASTHRDP-NSC Scholars’ Conference
February 21-22, 2013
Traders Hotel, Manila
Day 1: 21 February 2013
8:00 – 9:00 AM
Registration
Opening Program and Plenary Session 1
Session Chair: Dr. Ma. Luisa Mabunay
9:30 – 12:30 AM
9:00
9:15
9:20
9:40-10:25
10:25-11:10
11:10-12:30
12:30 – 1:30 PM
1:30 – 3:30 PM
3:30 – 3:45 PM
3:45 – 5:00 PM
5:00 – 7:30 PM
Ribbon Cutting and Opening of Poster Exhibit
Dr. Jose Maria P. Balmaceda
Dean, College of Science, UP Diliman
Hon. Mario G. Montejo
Keynote Message
Secretary, DOST
Science Consortium: A Brief History and Lessons Learned
Dr. Ester A. Garcia
Plenary Talk 1
President & Chief Academic Officer, University of the East
Developmental Stages in the Building of Graduate Programs and
Research: Challenges and Opportunities for the NSC
Plenary Talk 2
Fr. Bienvenido F. Nebres, S.J.
Chairman, Synergia Foundation
Dr. Lourdes J. Cruz
President, National Research Council of the Philippines, DOST
Panel Discussion on Dr. Florencia G. Claveria
Mentoring
Professor, Biology Department, College of Science, DLSU
Dr. Oscar B. Zamora
Vice Chancellor for Instruction, UP Los Baños
Lunch
Parallel Oral Presentations 1 (By cluster)
Coffee Break
Parallel Oral Presentations 2 (By cluster)
Poster Session/Cocktails
Welcome Remarks
Day 2: 22 February 2013
8:30 – 9:00 AM
Registration
Plenary Session 2
9:00 AM – 12:15 PM
9:00-9:45
9:45-10:30
Session Chair: Dr. Jose Maria P. Balmaceda
From Brownian Motion to Polymers - Random Walk with a Memory
Plenary Talk 3
Dr. Ludwig Streit
Professor, Bielfeld University, Germany
Ethics in Research and Publication
Dr. Evelyn Mae T. Mendoza
Plenary Talk 4
Retired Research Professor, UP Los Baños
10:30-10:45
10:45-11:30
Plenary Talk 5
Coffee Break
Intellectual Property and Science Research
Mr. Andrew Michael S. Ong
Former Management Executive - Intellectual Property Lawyer
11:30-12:00
12:00
12:15 – 1:15 PM
1:15 – 1:30 PM
Report of the NSC
Chair
Closing Remarks
Dr. Fabian M. Dayrit
Chair, NSC Steering Committee
Dr. FilmaG. Brawner
Director, SEI
Lunch
Distribution of Certificates
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