TABLE OF CONTENTS
PART ONE
(Introduction)
Vision, Mission . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Executive Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Brief History and Program Goals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1
2
4
PART TWO
(Program and Other Activities)
1. Research Programs and Publications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5
List of NSRI-funded Researches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5
Projects Terminated in 2010 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Projects Renewed in 2010 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Projects Approved for Funding in 2010 . . . . . . . . . . . . . . . . . . . . . . . . .
Externally-Funded Researches 2010 . . . . . . . . . . . . . . . . . . . . . . . . . . .
NSRI-Assisted Researches in 2010 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5
11
15
24
33
Publication and Creative Works . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
37
Extension Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
39
Technical Assistance and Advisory Services . . . . . . . . . . . . . . . . . . . .
39
Biological Research and Services Laboratory (BRSL). . . . . . . . . . . . . .
DNA Analysis Laboratory (DAL). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Microbiological Research and Services Laboratory (MRSL) . . . . . . . .
Research and Analytical Services Laboratory (RASL) . . . . . . . . . . . . .
39
45
68
84
Information Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Community Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Safety Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
96
97
97
1.1
1.2
2.
2.1
2.2
2.3
2.4
PART THREE
(Resources)
3.
Organization and Personnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.1
3.2
3.3
98
Organization and Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Executive Council . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Research Section. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Technical Support Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Administrative Support Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Personnel Inventory of the NSRI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
98
98
99
100
100
103
NSRI Staff Development in 2010 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Resource Generation and Allocations . . . . . . . . . . . . . . . . . . . . . . . . .
Fiscal Resources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
105
111
114
PART FOUR
(Conclusion)
Future Plans and Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
116
i
NATURAL SCIENCES RESEARCH INSTITUTE (NSRI)
Vision
A world-renowned National Center of Excellence for research
and technical services in the disciplines and multi-disciplines
of biology, chemistry, environmental, mathematical and
atmospheric sciences.
Mission
•
•
•
•
•
To provide opportunities for the development of high-caliber
scientific and technological human resources.
To attain world-class status for its research and scientific
contributions.
To attain world-class status for its various technical service
laboratories.
To take the initiative in the advocacy for the protection of
the environment.
To take the leadership in the advancement and application of
science and technology for the improvement of the quality
of life of the Filipino.
˜ Executive Summary ™
For 2010, a total of 28 projects were funded by the Natural Sciences Research Institute
(NSRI), i.e., 10 completed, 4 renewed, and 14 newly-approved or newly-implemented
researches. These projects came from the faculty researchers of the Institute of Biology,
Institute of Chemistry, Institute of Environmental Science and Meteorology, Institute of
Mathematics and an in-house laboratory. Priority was given to new PhDs or lateral entrants
with PhDs to give them a head start for research in the University. For longtime researchers,
publications and a good track record of research have become the main criteria for the
research grants. The University Research Associates (URAs) hired to assist these projects are
graduate students of the College of Science who are also supported in their courses through
the University’s Privilege to Study at Reduced Fees and the use of the Institute’s laboratories
and facilities for their thesis experiments. They are envisioned to add to the country’s
manpower pool of personnel with advanced training when they finish their degrees.
The NSRI also supported and assisted 11 research projects of in-house researchers
funded by outside agencies. A total of 6 research projects of some faculty researchers from
the Institute of Biology, Intitute of Chemistry and the National Institute of Physics have also
been assisted through the use of laboratory space, internet, electrical and water facilities. The
in-house laboratories have also been very active in pursuing their own research activities. The
Research and Analytical Services Laboratory (RASL) has maintained its accreditation to ISO
17025 since 2003. Among its many research activities, it has continued to implement projects
on POPs (Persistent Organic Pollutants), particularly on polychlorinated biphenyls and
organochlorine pesticides, polybrominated diphenyl ether in sediments and water, through
local and international collaborations. The Microbiological Research and Services Laboratory
(MRSL) continued to be a part of the big research program called PHIL-PHARMASEAS funded
by DOST-PCAMRD (Department of Science and Technology - Philippine Council for Aquatic
and Marine Research and Development) through its project entitled “Marine Microorganisms:
Characterization and Culture Optimization.” The DNA Analysis Laboratory (DAL) has continued
its work on forensic DNA analysis from different samples, particularly on the validation of
methods using different techniques. On June 2, 2010, the Institute through the DAL became a
member of the Asian Forensic Sciences Network (AFSN). The DAL has also started to expand its
work from human DNA analysis to the “Validation of Molecular Methods for the Species
Identification of Meat and Meat Products” initially funded by the Institute. It is envisioned that
other animal products will be studied in the following years for possible funding by outside
agencies in order to address the country’s need for molecular analysis, particularly, by the
animal/meat industry. The Biological Research and Services Laboratory (BRSL) continued to be
a collaborator of the UP Diliman Archaeological Studies Program on their researches on
“ancient DNA” and archaeobiology.
The “DA-BAR/UP-NSRI Post-Doctoral and Senior Scientist Research Fellowship in Basic
Research for Agriculture and Fisheries” implemented the research project of Post-Doctoral
Fellow Dr. Ruel M. Mojica of the Cavite State University on the topic “Influence of Roasting on
the Chemical Attributes and Antioxidant Activity of Philippine Coffee.” This was started on
June 15, 2010 and is expected to be finished by June 14, 2011. Funds were provided by the
Department of Agriculture – Bureau of Agricultural Research as its support to basic research.
This research fellowship is the first of its kind in offering a local fellowship to faculty and
researhers from other institutions who will spend a year of full-time research working on basic
problems that will have important applications in agriculture and fisheries. The UP NSRI
implements and monitors the project up to its completion. This is also an opportunity for the
University to open its doors to faculty and researchers from other Philippine institutions for
updating and/or further training in research dealing with local problems.
Technical extension services continued to be rendered by the in-house laboratories of
the Biological Research and Services Laboratory, DNA Analysis Laboratory, Microbiological
Research and Services Laboratory and the Research and Analytical Services Laboratory.
Funds generated were used to purchase minor equipment and additional supplies for the
2
service laboratories. They were also used to hire non-UP contractual personnel who served as
additional research associates and analysts in order to augment the existing small number of
personnel. The resources generated have been very useful in filling in a lot of the financial
needs due to limited funds from the Institute’s MOOE allotment and the lack of available items
in the University. Training courses were also conducted to expose students to different
laboratory protocols and to update teachers, researchers and analysts from government as
well as private laboratories all over the country. Students from different parts of the country
were likewise able to avail of laboratory tours as well as summer internship and technical
assistance to thesis and research projects.
The Safety Program of the Institute continued to be implemented in order to make sure
that laboratory activities conform to safety guidelines. The Chemical Waste Management
Seminar as well as Laboratory Safety Orientation was conducted twice in 2010 which also
included biological/microbiological and fire and building safety lectures. Proper use and
handling of small laboratory animals were also added to the Safety Program. New University
Research Associates and new Project Leaders were also given an Orientation on purchase
procedures and other administrative aspects of research in 2010. The Institute also started to
implement the 5S Program in office work places to ensure organized and efficient service of
the personnel.
ERNELEA P. CAO, Ph.D.
Directo r
3
BRIEF HISTORY AND PROGRAM GOALS
June 19, 1964
:
Republic Act 3887, signed by President Diosdado Macapagal for the
establishment of the Natural Sciences Research Center (NSRC) as
amended by Republic Act 6069 (1969).
February 1965
:
U.P. President Carlos P. Romulo organized the Executive Advisory
Council with the task of getting funds for the Center.
July 1971
:
NSRC was first opened to researchers.
November 13, 1972 :
Formal inauguration and dedication ceremonies of the UP-NSRC by
U.P. President Salvador P. Lopez.
March 23, 1983
:
Executive Order No. 889, issued by President Ferdinand E. Marcos,
organized the Natural Sciences Research Institute (NSRI) from the
Natural Science Research Center (NSRC).
May 26,1983
:
Approval of the Implementing Guidelines for the creation of the NSRI
out of NSRC by the UP Board of Regents.
July 2, 1985
:
Executive Order No. 7, signed by UP President Edgardo J. Angara
provided for the organizational structure and staffing pattern of the
NSRI.
December 18,1986 :
Executive Order No. 6 signed by UP President Edgardo J. Angara
amended EO No. 7 to include interdisciplinary programs.
:
The NSRI building was named Miranda Hall in honor of the late Prof.
Bienvenido T. Miranda, the Institute's first Director.
February 28, 2000
The NSRI serves as the national center of excellence for the advancement,
dissemination, and application of knowledge in Biology, Chemistry, Environmental and
Atmospheric Sciences and Mathematics. It is mandated to:
a.
undertake research in Biology, Chemistry, Environmental and Atmospheric Sciences,
Mathematics, and organize interdisciplinary research programs.
b.
support the graduate programs of the College of Science in these fields of study.
c.
provide extension and other services on request and when feasible do research
needs such as consultancies, research, training, technical support, analytical
support, printing services to researchers, other units of the University, and other
institutions and agencies, both government and private.
d.
disseminate research findings
conferences and seminars.
through
scientific
publications,
workshops,
4
1. RESEARCH PROGRAMS AND PUBLICATIONS
1.1 LIST OF NSRI-FUNDED RESEARCHES
RESEARCH SUPPORT: MOOE, FACILITIES, HONORARIA AND SALARIES OF PROJECT PERSONNEL
PROJECTS TERMINATED IN 2010
BIOLOGY
1)
Project Title
:
Genetic Structure of Populations of the Philippine
Endemic Silver Perch Leiopotherapon plumbeus
(Kner, 1894) Based on Mitochondrial DNA Variation
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Collaborators
:
:
Personnel
:
BIO-09-2-01
NSRI
2010 – P 459,718
2009 – P 444,263
January 1, 2009 – December 31, 2010
Dr. Jonas P. Quilang
Dr. Ian Kendrich C. Fontanilla
Dr. Zubaida U. Basiao
1 University Research Associate I
Abstract:
Despite the presence of morphological variation between native populations
of silver perch in Laguna de Bay and transplanted populations in Taal Lake and
Sampaloc Lake (Quilang et at. 2007), very low genetic variation was observed in both
native and transplanted populations using isozymes (Quilang et al. 2008). Since
mitochondrial DNA is a more sensitive marker (i.e., it is more variable) than isozymes, it is
hoped that a more accurate picture of the genetic structure of the native and
transplanted populations will be obtained. Furthermore, three additional populations
from Paoay Lake, Lake Buhi, and Maasin River in Candaba, Pampanga will be
included to assess levels of genetic variation in other populations of this species.
2)
Project Title
:
Profiles of Entamoeba histolytica-Specific
Immunoglobulin A, G, and M in Human Sera
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Co-Project Leader
Personnel
:
:
:
:
BIO-09-2-03
NSRI
2010 – P 513,018
2009 – P 442,913
January 1, 2009 – December 31, 2010
Dr. Windell L. Rivera
Mr. Herbert J. Santos
1 University Research Associate I
Abstract:
Amebiasis is an infection with the protozoan Entamoeba histolytica which
causes 100,000 deaths per annum, placing it second only to malaria based on
mortality caused by protozoan parasites (WHO, 1997). In the Philippines, E. histolytica
infection has not been well- characterized. This study aims to conduct profiling of IgA,
5
IgG, and lgM antibodies in humans infected with E. histolytica. Further understanding
of the roles of the three classes of antibodies in the immune response against E.
histolytica infection among Filipinos is warranted to provide a better insight on the
mechanisms that permit or inhibit invasive amebiasis. This could also be a precedent
for the development of new diagnostic markers and possible treatment against
amebiasis.
3)
Project Title
:
Reproductive Biology of the Silver Perch
Leipotherapon plumbeus (Teleostel: Teraportidae)
from Selected Freshwater Habitats in Luzon
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Collaborators
:
:
:
Personnel
:
BIO-09-2-06
NSRI
2010 – P 381,718
2009 – P 398,013
January 1, 2009 – December 31, 2010
Dr. Luis Maria B. Garcia
Dr. Evelyn Grace de Jesus-Ayson
Mr. Manuel A. Laron
1 University Research Associate I
Abstract:
(Manuscript Title:
Influence of Sex and Gonad Condition on the Length-Weight
Relationship of the Silver Perch Leiopotherapon plumbeus)
Aspects of the reproductive biology of the teraponid silver perch
(Leiopotherapon plumbeus) in the Candaba wetland in Central Luzon, Philippines were
investigated. A significant length-weight relationship (LWR) was observed in male and
female fish collected monthly in 2009. Female fish showed a higher b value (3.03) than
males (2.82), indicating that female silver perch were slightly heavier than males of
similar length. The LWR of female fish and the pooled sexes revealed isometric growth
whereas, males exhibited negative allometric growth. Gonad growth and gut index
(weight of the digestive tract relative to eviscerated body weight) varied during the
year. Seasonal testicular and ovarian gonadosomatic index peaked in June as the gut
index of both sexes began to increase and remained elevated for the duration of the
wet season (June-October). In addition to gut index, the relative condition factor of
females, but not males, was negatively correlated with ovarian growth, suggesting
a possible difference in the allocation of food energy for somatic and gonad growth
between the sexes. Overall, these results demonstrate that a significant LWR of male
and female silver perch allows for the estimation of biomass from length at least
within the range reported in this study. As well, peak in the gut index coincides with
the flooding of the wetland during the wet
season for gonadal growth during the subsequent dry season.
4)
Project Title
:
Immunomodulatory-Based Screening of Crude
Extracts from Indigenous Plant Species Collected from
Kanawan, Morong Batan Forest Area
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
BIO-08-3-01
NSRI
2010 – P 393,718
2009 – P 390,513
2008 – P 372,781
January 1, 2008 – December 31, 2010
Dr. Elena S. Catap
1 University Research Associate I
6
Abstract:
(Publication Title:
Immunostimulatory Effects of Uncaria perrottetii [A.Rich] Merr.
[Rubiaceae] Vinebark Aqueous Extract in Balb/C Mice)
The optimum concentration was determined to be 50 µg/mL in the in vitro
assays. At this concentration, Uncaria perrottetii extract stimulated peritoneal
phagocyte activation, produced a significant increase in the activity of phagocytic
cells from the spleen and promoted splenic cellular proliferation with or without
lipopolysaccharide (LPS) when compared with the PBS-treated cells (negative control).
Moreover, cells treated 50 µg/mL of Uncaria perrottetii increased macrophage
respiratory burst activity that was comparable to that of the phorbol myristate acetatestimulated splenic macrophages.
In all immune assays undertaken in the in vivo experiment, the Cy-treated mice
showed significantly lower response when compared with the PBS-treated mice.
Significant improvement in peritoneal cell activation, phagocytic activity and cellular
proliferation was exhibited by the U+Cy-treated mice when compared with Cyinjected mice. The extract from Uncana perrottetii also significantly enhanced
respiratory burst and plasma lysozyme activity compared with the Cy-injected mice.
Based on the results of both in vitro and in vivo trials, Uncaria perrottetii extract
has immunopotentiating activities on the innate immunity of Balb/C mice and the
extract could potentially reverse the immunosuppressive effects of Cy. However, the
potential of the plant as source of bioactive products and metabolites for
drug development still has to
be fully investigated.
CHEMISTRY
5)
Project Title
:
Potential Anti-Asthma Compounds from Selected
Philippine Plants
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
CHE--09-2-02
NSRI
2010 – P 456,652
2009 – P 380,513
January 1, 2009 – December 31, 2010
Dr. Christine L.C. Hernandez
1 University Research Associate I
Abstract:
Inhibition of the formation of the products of lipoxygenase catalysed
oxygenation has been considered for the development of new therapies for airway
inflammation particularly asthma. One of the well known promoters of the emergence
of pro-inflammatory mediators is 15-lipoxygenase. Previously identified inhibitors of 15lipoxyganase function via a redox mechanism. But these compounds have been
associated with toxicity and non-specificity due to their redox activity. Hence, there is
a need to develop inhibitors of 15-lipoxygenase.
The structure of the isolated
compound may provide a new skeleton for the development of a better antiinflammatory drug which is more efficacious and has fewer side effects.
7
6)
Project Title
:
Synthesis of Ionophores with Amide, Imine, and OxaAza Moieties as Sensing Materials Ion Detection
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Co-Project Leader
Personnel
:
:
:
:
CHE--09-2-07
NSRI
2010 – P 442,118
2009 – P 371,013
January 1, 2009 – December 31, 2010
Dr. Susan DR. Arco
Dr. Florian R. Del Mundo
1 University Research Associate I
Abstract:
A series of ionophores with varying functionalities; cyclic and acyclic amide,
cyclic imine, and aza-crown ether, has been synthesized. Wth the objective of making
use of these ionophores in the development of metal ion sensors, their metal ion
selectivity has been evaluated through computational methods MM2 and PM3. A
verification of the selectivities of these ionophores s currently being conducted through
wet chemistry through the separate solution method.
7)
Project Title
:
Synthesis and Characterization of Polyurethane
Polymers Based on (ω -1) Coconut Fatty Acids
Copalymerized with Simple Polyols and Hydroxyacids
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
CHE--09-2-09
NSRI
2010 – P 454,458
2009 – P 384,632
January 1, 2009 – December 31, 2010
Dr. Florentino C. Sumera
1 University Research Associate I
Abstract:
Hydrolytic stability and cytotoxicity test of a poly(ester-urethane) (PEU) were
performed to evaluate its potential as a new elastomeric biomaterial good for
scaffolding purposes. PEU was synthesized from hydroxylauric acid and lactic acid
in a condensation polymerization reaction with 1,4-butanediol and stannous octoate,
followed by chain extension with hexamethylene diisocyanate. In the hydrolysis
test, dry and wet polymer weights of the PEU films (10 mm x 10 mm) incubated at
37°C in phosphate buffer saline (PBS) solution (pH 7.4) were monitored at specific
time periods. From the observed increase in water uptake of PEU samples with
respect to time of soaking, the material was observed to be bulk eroding rather
than surface eroding. In the cytotoxicity test, disc specimens of PEU films (5mm in
diameter) were prepared and extracted for 24 hours in minimum essential medium
(at 37oC and pH 7.4) supplemented with fetal bovine serum and antibiotic.
Extracts were placed on human white blood cell monolayers and cell
numbers were analyzed via dye exclusion assay. The difference in cell numbers
between the non-exposed medium and medium exposed to PEU was
observed to be insignificant confirming the polymer to be non cytotoxic
and considered as potential biomaterial for biomedical application.
8
ENVIRONMENTAL SCIENCE AND METEOROLOGY
8)
Project Title
:
Wavelet De-Noising Applied to Cloud Height
Determination from Portable Automated Lidar Data
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Collaborator
:
:
:
ESM-09-1-01
NSRI
2010 – P 61,000
2009 – P 71,000
January 1, 2009 – December 31, 2009
Dr. Gerry Bagtasa
Mr. Nabuo Takaushi Hiroski Ku
Abstract:
The portable automated lidar has been effective in observing macro and
microscopic atmospheric parameters such as atmospheric boundary layer height,
cloud height, rain drop velocity, atmospheric extinction and backscattering
coefficient, etc. The continuous monitoring also enabled PAL in observing cloud
dynamics and aerosol optical properties in the troposphere. However, this has also
indicated that the detection range is rather limited for the daytime data because of
the background noise from the sky radiance. This study aims to improve lidar data
with
wavelet
signal
analysis
especially
for
daytime
observations.
9)
Project Title
:
Photo-Identification of Selected Small Cetaceans in
Southern Tañon Strait
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
ESM-08-2-01
NSRI
2010 – P 419,718
2009 – P 410,513
2008 – P 385,081
January 1, 2008– December 31, 2010
Dr. Lemmuel V. Argones
1 University Research Associate I
Abstract:
(Manuscript Title:
Southern Tañon Strait Spinner Dolphins Photo-Identification)
Our study site is Tañon Strait. Tañon Strait is a narrow, deep channel located
between the islands of Negros and Cebu. This Strait is about 200 km long and
connects the Visayan Sea to the Bohol Sea and bordered by about 41 municipalities
and cities.
Tañon strait harbors 15 species of marine mammals, including 14 cetaceans
and the dugong. This is one of the major reasons why Tañon Strait was declared as a
Protected Seascape in 1998. In 1995, in the southern section of the Strait, dolphin
watching tourism was initiated by Bais City, Negros Oriental.
A photographic identification study of the spinner dolphins in the area has
been going on since 2004. To date, there are about 297 unique individuals of spinner
dolphins identified in the area. This photo-identification study is actually a part of a
larger project entitled ‘Ecology and conservation of cetaceans in southern Tanon
Strait’.
The ultimate goal of the project is to conserve and protect the cetaceans in
the area by gathering scientific data that will be utilized for the development of a
9
conservation plan through a participatory management process involving local
stakeholders.
10)
Project Title
:
The Ecological Consequences of Development:
Landscape Changes and the Impact of Bird
Biodiversity in the UP Campus and Nearby Areas
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
ESM-09-1-02
NSRI
2010 – P 329,218
2009 – P 300,513
January 1, 2009 – December 31, 2009
Dr. Benjamin Vallejo, Jr.
1 University Research Associate I
Abstract:
The project will be an important contribution to ecological impact studies in the
Philippines and is one of the first studies to assess impact on a landscape level in a
tropical terrestrial environment. The project also will be of value in assessing the
environmental impacts of development in the University of the Philippines. The
passage of the new UP Charter into law and the celebration of the University
centennial will in no doubt give the university more opportunities to receive funding to
develop its real estate assets. This is now happening as areas in the campus have
been developed due to private sector investment, as science parks and from
additional National government appropriations as in the National Science Complex.
The University of the Philippines Diliman campus is an excellent urban laboratory
to assess the effects of urban development on tropical biodiversity. This is due in part
to a long standing research effort by the defunct departments of Botany and Zoology
(now the Institute of Biology) to document the Diliman biotic environment, although
many of these studies were descriptive. The previous studies on the bird community of
the campus were intensive, quantitative and ecological statistical estimates are now
available. These can provide important information to assess the immediate impact of
campus development, especially in the National Science Complex. The previously
collected data can act as statistical priors to predict the fate of biodiversity in the face
of campus development.
The
results of this study can serve as a model to assess the effects of
urbanization in other cities, in the Philippines and overseas.
10
PROJECTS RENEWED IN 2010
BIOLOGY
1)
Project Title
:
Sexual Dimorphism in Genetically Improved Nile
Tilapia (Oreochromis niloticus L.)
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
BIO-09-2-05 (extension)
NSRI
2010 – P 369,718
2009 – P 330,513
January 1, 2009 – December 31, 2010
Dr. Zubaida U. Basiao
1 University Research Associate I
Status of the project:
The use of hormones to sex reverse tilapia fry in favor of producing all male
tilapia for grow-out is widely practiced in the tilapia grow-out industry in the Philippines.
The reason for this is the observed fast growth of males when the females start mouthbrooding the fry. Grow-out culture in earthen ponds takes about four to six months.
Some farmers who breed tilapia have observed that four to six month old genetically
improved Nile tilapia showed delayed sexual maturation compared to conventionally
bred tilapias. According to Bolivar (1998), sexual maturation generally stars around 16
weeks old in conventionally bred tilapias. This study aims to study sexual dimorphism in
genetically improved tilapia by comparing the growth rates of males and females that
are growing together in the same environment without tagging the individual fish. The
gonadal maturation stages of four- to nine-months old male and female tilapia will be
characterized and correlated with size dimorphism in genetically improved Nile tilapia.
Use of hormonally sex reversed tilapia in grow-out systems can be minimized if farmers
are shown that the size advantage of males over females is not significant during the
four- to six-month culture period. Schreiber et al. (1998) have shown that individually
reared female tilapia can grow faster than males.
NSRI
2)
Project Title
:
Validation of Molecular Methods for the Species
Identification of Meat and Meat Products
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
:
:
NSR-09-1-01 (extension)
NSRI
2010 – P 150,000
2009 – P 150,000
January 1, 2009 – December 31, 2009
Dr. Maria Corazon A. de Ungria
Status of the project:
The study aims to validate Polymerase Chain Reaction-Restriction Fragment
Length Polymorphism (PCR-RFLP) as a tool for identifying species origin of meat and
derived products. Cytochrome b amplicons from pork and pork products in an earlier
study will be digested with HaeIII restriction enzyme. The restriction profiles of pork and
pork products will be compared with the restriction profiles generated using cattle,
chicken, goat, carabao, and human DNA. The success of PCR-RFLP analysis to
differentiate between species will be indicated by the generation of distinct restriction
profiles for each species.
11
NSRI COMMITMENT PROJECTS
3)
Project Title
:
Validation of DNA Typing Procedures and Assessment
of Genetic Relationships among Filipino
Ethnic/Language Groups
(Services of URAs are needed for the smooth operations of
DNA Analysis Laboratory)
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
NSR-97-2-04
NSRI/Office of the Chancellor, UPD
2010 – P 1,030,872 (personnel salary)
2009 – P 938,052 (personnel salary)
2008 – P 1,017,124 (personnel salary)
January 1, 1997 – continuing project
Dr. Maria Corazon A. de Ungria (1999 – present)
4 University Research Associate I
Status of the project:
DNA analysis is a powerful tool for forensic human identification. Any biological
sample i.e., blood, bone, tooth, tissue, may be used to generate a DNA profile.
Through the past five years, including 2010, the laboratory had an increasing demand
in cases of varying complexities. Continuous efforts were given in the validation of
procedures for environmentally challenged samples, with emphasis on the application
of forensic DNA technology for samples like exhumed bones and teeth, biopsies and
\post-mortem human remains. In forensic applications, biopsy tissues may be used as a
source of genetic information for personal identification or filiation testing. DNA
technology may also be used to establish identity or verify issues of specimen mix-up or
fraudulent identities. The laboratory proposed to study different DNA extraction
techniques to increase efficiency of recovering and isolating DNA from formalin fixed
paraffin embedded tissue (FFPET), cigarette butts, compromised tissue samples, saliva,
exhumed bone samples. DNA from these samples would serve as template for
autosomal as well as mitochondrial DNA analysis. Factors such as the presence of
inhibitors, nuclease-producing microorganisms and very small amounts of DNA must
also be addressed.
In addition, the laboratory addressed the problem of fraudulent adulteration
and/or substitution of meat products in Philippine markets. Fraudulent adulteration and
substitution of declared animal products with other species of lower commercial value
has been a growing concern for the National Meat Inspection Service (NMIS) of the
Department of Agriculture, the government agency mandated to conduct routine
inspection of meat that are sold commercially. Current testing procedures in the
Philippines involve visual inspection and subsequent chemical analysis of meat
collected from random sampling of different points of the meat chain, e.g. from farm
to market. The validation of DNA-based methods of meat species identification that
are more discriminating, reliable, sensitive, and cost-effective is needed to improve
current methods of detection. At present, DNA-based methods are not routinely used
for proper identification of animal meat products during random meat inspection in
the Philippines. The results of the study may also be used in the formulation of guidelines
on meat species identification under The Philippine National Standards on Halal Food
(PNS 2067:2008).
In addition, the Laboratory continued its involvement in a number of
collaborative efforts that will increase the strength of the Philippine population
database, understand the peopling of the Philippines and its importance in the
Philippine population genetics. To understand the genetic diversity of the Philippine
population, a study was conducted in collaboration with Leipzig School of Human
Origins-Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany to
12
evaluate the Y-chromosome genetic affinities of Filipino language groups. A total of
390 Filipino language group samples all over the Philippines were analyzed.
The laboratory had also put considerable effort to increase its international as
well as local linkages. A Memorandum of Agreement was signed between the
University of the Philippines Diliman and the University of Granada to partner in
promoting the use of science, particularly DNA technology, in the war against child
trafficking. In fact, the UP-NSRI DNA Analysis Laboratory was identified as the Philippine
reference laboratory of the international initiative known as DNA-Prokids (www.dnaprokids.org). The key proponents of DNA Pro-kids International – Dr Jose Lorente and Dr
Arthur Eisenberg- delivered lectures in UP in January 2010 in order to disseminate
information about the program. Present in the forum were members of the Philippine
Interagency Agency Council Against Trafficking (IACAT), namely, former DOJ
Undersecretary Ricardo Blancaflor, and former DSWD Secretary Esperanza Cabral.
In June 2010, the laboratory’s application to the Asian Forensic Science
Network (AFSN) was successful. In fact, the organization approved the membership of
the entire Natural Sciences Research Institute (NSRI) because of the work which was
being conducted in the laboratory. AFSN is composed of representatives from different
Asian countries like Malaysia, Thailand, Indonesia, Singapore, Brunei, Vietnam and
Korea who meet every year to share ideas, strengthen ties and improve collaboration
in the East Asia Pacific Region and to confer on the forensic development policy and
international guidelines for laboratories that utilize forensic technology.
Lastly, the laboratory also took part in disseminating more information to various
government agencies, NGOs and the private sector through lectures, open forum and
participation in media activities. The laboratory was able to form informal linkages with
the Philippine Dental Association (PDA), the Armed Forces of the Philippines (AFP), the
Commission on Human Rights (CHR), the Department of Social Welfare and
Development (DSWD) and Asia Against Child Trafficking (Asia-ACT). The laboratory
aims to work on formalizing the initial linkages with these groups in the future.
4)
Project Title
:
Chemical Waste Management Program of NSRI
Project Code
Funding Source
Amount of Funding
:
:
:
Duration of Project
Project Leader
Personnel
:
:
:
:
NSR-00-1-05
NSRI
2010 – P 234,513 (personnel salary and lab supplies)
2009 – P 234,513 (personnel salary and lab supplies)
2008 – P 216,781 (personnel salary and lab supplies)
January 1, 2000 – continuing program
Dr. Ernelea P. Cao
1 Pollution Control Officer
1 Chemical Safety and Waste Mgt. Associate
Status of the project:
The NSRI is continuously generating chemical waste from the operations of its
research and technical laboratories. Thus, the NSRI is expected to sustain its chemical
waste management program. In compliance with requirements of RA 6969 (Toxic
Substance and Hazardous and Nuclear Waste Control Act of 1990), the NSRI has been
a DENR-registered hazardous waste generator since Y2000.
The main focus of the project was to monitor the waste generated at the
Institute and to ensure safe storage until it is due for treatment by accredited waste
treaters. For Y2010, the unidentified chemical wastes inside the laboratory rooms were
minimized by the implementation of proper turn-over of responsibilities between URA
during resignation/end of contract to the incoming new URA with proper
documentation including inventory of supplies/chemicals and equipment, as well as
identification of chemical wastes before the issuance of clearance. Random
13
laboratory inspection was done also for monitoring purposes. Collection and proper
storage of chemical wastes from the research and technical laboratories were still
done.
The project was able to serve a total of 9 requests for waste collection mainly
from URAs and has collected 186.56 liters of assorted hazardous and toxic wastes. Two
(2) batches of collected wastes for treatment were sent to our accredited waste
treater.
There was a continuing implementation of the chemical exchange program of
the Institute in 2010 and a total of 70 requests were served (from students = 44, URAs =
14, CS Faculty = 6, In-house labs= 3 and Outsiders = 3).
The project was also able to prepare and submit on time the quarterly
Hazardous Waste Generation Report to the Department of Environment and Natural
Resources – Environmental Management Bureau (DENR-EMB) for Y2010.
14
PROJECTS APPROVED IN 2010
BIOLOGY
1)
Project Title
:
Affinity Chromatography, Molecular Weight Analysis
and Agglutination Assay of Fucolectin from the Gut of
Oreochromis niloticus L.
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Personnel
:
:
:
:
:
:
BIO-10-1-01
NSRI
2010 – P 461,693
January 1, 2010 – December 31, 2010
Dr. Anacleto M. Argayosa
1 University Research Associate I
Description of the project:
One of the primary defenses of fish against pathogens is in the mucosal lining of
the gut. Macrophages and antigen presenting cells involved in innate immune
response express proteins that bind glycans and help in the neutralization of
pathogens. Some of these proteins that bind pathogen associated molecular patterns
or PAMPs are lectins that bind to specific glycan moieties on the surface of viruses and
pathogenic microorganisms. We have isolated these fucose-binding proteins from the
sera and detected similar proteins in immune-related tissues of the cichlid such as in
the spleen and head kidney . We discovered significant amounts of fucose-binding
proteins from the gut of the cichlid and propose the isolation and characterization of
the fucolectins from the gut to provide insights on the role of these carbohydratebinding proteins in mucosal immunity. Not much is known about the presence of
fucolectins in the cichlid especially in the gut. It is possible that these lectins may act as
opsonins that coat pathogens and enhance phagocytosis of the microbes. This
recognition and binding processes are important in the later cascade of cell-mediated
and humoral immunity. Understanding the role of these proteins in the gut could be an
avenue in promoting higher antigen uptake for induction of adaptive immune
response. It may also be targeted as carrier in developing oral vaccines carrying
antigenic glycans that are specifically recognized by fucolectins. The binding of lectin
may trigger the phagocytic action of the macrophages of the hind gut that promote
the activation of antigen presenting cells that initiates specific antibody production.
Moreover, fish lectins, such as the fucolectins, may be explored in developing
improved tools for monitoring disease resistance against various infectious diseases in
fishes. It is proposed that these family of fucose-binding proteins could be tapped as
Biomarkers for fish health.
2)
Project Title
:
Transmission Electron Microscopy and
Immunohistochemical Evaluation of the
Angiosuppressive Activtiy of the Hexane and Ethyl
Acetate Fractions of Ardisia sp. from Kanawan,
Morong, Bataan
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Personnel
:
:
:
:
:
:
BIO-10-2-02
NSRI
2010 – P 455,718
January 1, 2010 – December 31, 2011
Dr. Annabelle A. Herrera
1 University Research Associate I
15
Description of the project:
The NSRI and the UP System Research and Creative Scholarship Program
provided this proponent financial support for the screening of endemic medicinal
plants from Barangay Kanawan, Morong, Bataan for their antiteratogenic and
antiangiogenic properties using the duck in ovo assay. This research proposal will serve
as a starting point for more advanced and detailed research for naturally occurring
drugs from endemic plants for inhibition of tumor angiogenesis. This study will prepare
hexane and ethyl acetate fractions from Ardisia sp. and analyze significant
antiangiogenic ability and possible mechanism/s of action. Data generated from the
project can open up new areas of research on new anticancer treatments and be
utilized by the health and pharmaceutical sectors, for cancer cure. Ardisia sp.,
might be a natural source of antiangiogenic compounds. This study can provide
leads for new antiangiogenic drugs for novel cancer treatment.
3)
Project Title
:
Isolation of Compounds Cytotoxic to Human Cancer
Cells from Semipure Fraction of Leaf Extracts from
Voacanga globosa (Blanco) and Aglaia Ioerii
(Blanco)
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Personnel
:
:
:
:
:
:
BIO-10-1-03
NSRI
2010 – P 455,718
January 1, 2010 – December 31, 2010
Dr. Sonia D. Jacinto
1 University Research Associate I
Description of the project:
A project of the proponent, funded by the UP-OVCRD, yielded a number of
compounds with potential anticancer activity from two plants, Voacanga globosa
Blanco (Merr.) and Aglala loheri Blanco, which are endemic/indigenous plants from
the Philippines. The number of potential anticancer compounds was deduced from
the preparatory TLC profile and HPLC profiles of the plant fractions. The anticancer
activity of these prep TLC bands and HPLC peaks were established by the MTT
cytotoxicity assay using two human cancer cell lines, lung adenocarcinoma (A549)
and colon carcinoma (HCT 116), both cell lines obtained from the American Type
Culture Collection. (ATCC, Manassas, VA, USA). Since the OVCRD funding ends on
October 2009, the most possible and realistic accomplishment from the remaining
funds would be to be able to purify and chemically characterize at most one pure
compound from each plant. In this light the rest of the compounds with strong
cytotoxic activity will remain unknown or unexplored. This proposal would like to
continue the purification so that additional and possibly compounds can be isolated.
CHEMISTRY
4)
Project Title
:
Determination of Cadmium and Lead in Breast Milk
Samples. Method Optimization, Validation and
Analysis of Actual Samples
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
:
CHE-10-2-01
NSRI
2010 – P 413,718
January 1, 2010 – December 31, 2011
Dr. Irene B. Rodriguez
16
Personnel
:
1 University Research Associate I
Description of the project:
The proposed project will, as specified in the objectives, be focused in two
major parts: the method optimization and application in real samples. The first part will
be beneficial in setting up a working method which may be used for routine
monitoring, if there need be, of elements in milk samples and similar biological/clinical
samples. The experience from optimization of this method will clearly provide insights
on how other samples may be analyzed. The second part of the work will provide the
benchmark values of elements in milk samples. At the very least, the results from this
work will give information as to the degree of exposure of babies to toxic elements like
cadmium and lead. This work is aimed towards providing information as to the effect
like dietary intake, lifestyle and occupational exposure to the levels of cadmium and
lead in breast milk samples.
5)
Project Title
:
Determination of Decabromodiphenyl Ether and
Tetrabro-mobisphenol-A in Indoor Dust by
Ultrasonication-HPLC/UV
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Personnel
:
:
:
:
:
:
CHE-10-2-03
NSRI
2010 – P 150,000
January 1, 2010 – December 31, 2011
Dr. Ma. Phythias B. Espino
1 University Research Associate I
Description of the project:
This project involves the development and optimization of an ultrasonic
extraction and a HPLCUV determination method for decabromodiphenyl ether
(decaBDE) and tetrabromobisphenol-A (TBBPA) in indoor dust. These analytical
methodologies will be used to study the occurrence and levels of the flame retardant
compounds decaBDE and TBBPA in indoor environments in the Philippines, specifically,
in homes and internet cafes.
Brominated flame retardants (BFRs), including decaBDE and TBBPA, have been
detected in the different environmental matrices such as air, water, soil, sediment,
sludge and biota, as well as in human milk. BFRs are expectedly found in the
environment because they eventually leak from flame retardant-laden products such
as electrical and electronic equipment, paints, plastics, furnishing or mattress foams,
carpets, etc. In the Philippines, there is lack of information on the public’s potential
exposure to these compounds. This study will particularly look at the occurrence and
levels of decaBDE and TBBPA in indoor environments. Among the brominated flame
retardant compounds, decaBDE and TBBPA are the more challenging to study.
DecaBDE that easily degrades in a normal gas chromatographic system requires
special techniques such as the use of shorter columns and analysis time, while TBBPA
requires derivatization before GCECD/MS/MS-MS analysis.
For this project, an
ultrasonication extraction with HPLC-UV determination method involving minimal
sample preparation and without derivatization will be developed for decaBDE
and TBBPA in dust samples. The method
will be used in determining the
occurrence, concentrations and possible exposure of Filipinos to these compounds in
indoor environments such as their homes and in internet cafes.
17
6)
Project Title
:
Toxicological Assessment of Extracts of Indoor Dust,
Laboratory and Faculty Room Air Samples and
Diesel/Biodiesel Exhaust - Impacted Air Using
Allium cepa Cytogenetic Assay
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Co-Project Leader
Collaborator
Personnel
:
:
:
:
:
:
:
:
CHE-10-2-04
NSRI
2010 – P 468,118
January 1, 2010 – December 31, 2011
Dr. Carina Ramos
Dr. Leni L. Quirit
Ms. Raquel O. Rubio
1 University Research Associate I
Description of the project:
In view of the construction of the UP Diliman Science Complex, specifically, two
buildings for the Institute of Chemistry, sampling and analysis of laboratory and faculty
room air and indoor dust to determine potential environmental toxicity is relevant to
establish baseline conditions at the start of room occupancies, with subsequent
monitoring with time. These can also be done for other buildings in the Complex, and
for other environments like homes and internet cafes.
In view also of the passage of the law mandating the use of biodiesel blends in
diesel vehicles, chemical and genotoxic potential of pollutants from the diesel
vehicular exhausts also need to be assessed to determine if there is actual
improvement in the diesel vehicular exhaust-impacted air with the use of biodiesel. The
presence of oxygenates, for example, in biodiesel may potentially increase NOx
emissions.
7)
Project Title
:
Selective Electroless Copper Deposition in Modified
Polymer Surfaces
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Personnel
:
:
:
:
:
:
CHE-10-1-05
NSRI
2010 – P 447,018
January 1, 2010 – December 31, 2010
Dr. Guillermo M. Nuesca
1 University Research Associate I
Description of the project:
With the increasing demand every year for fabricating flexible and wearable
electronic devices there is a need for a simple, economical and environment-friendly
method of generating patterned polymeric substrates with fine resolution. This project
was aimed to develop an approach to selectively deposit Cu film on modified polymer
surfaces via electroless deposition utilizing microcontact printing to define metallization
pattern. Electroless deposition is a solution based electrochemical process of reducing
ions to metals onto a substrate in the absence of an electric current. This low-cost
method has been widely used in the microelectronics and related industries in
depositing metal films onto different types of surfaces, both conducting and nonconducting. The traditional electroless metal plating involves a bath containing the
metal-precursors (metal ions, supporting electrolytes and additives) and a solidcatalyzed oxidation process of the reducing agent (normally formaldehyde).
Palladium metal is commonly used as the catalyst to initiate the electrochemical
reaction and to help convert the metal-ions to deposited metal on the substrate. In
this research, an alternative, more environmental-friendly solution chemistry has been
explored for the electroless deposition of copper on different surfaces. Instead of using
the conventional Pd/Sn catalyst, the Cu-deposition has been activated using a
18
synthesized poly(diallyl dimethylammonium) chloride (PDDA)-protected Cu/Pd
nanoparticles. The electroless deposition of Cu has utilized a more benign plating bath
solution: dimethylamineborane as the reducing agent instead of formaldehyde, which
has health hazards. The use of PDDA-protected Cu/Pd nanoparticles activation has
eliminated the need for a Sn solution, also reported to be hazardous. The new
procedure has also lowered the cost of electroplating by minimizing the use of the
expensive Pd metal. The added benefit with the use of this new electroless plating
system is the potential for selective deposition of Cu. This research has addressed the
problem of localizing the catalyst in the target area for selective metal deposition on
polyimides by using a surface modification technique. Copper doped polyimide (PI)
films were also prepared via cycloimidization of the homogenous solution of anhydrous
copper salt and polyimide precursor, poly(pyromellitic dianhydride-co-4,4′-oxydianiline)
amic acid (PMDA/4,4’-ODA), through thermal annealing. As the polymer backbone
and the copper layer are formed concurrently, the substrate is more stable to distortion
or decomposition. The incorporated Cu will serve as the necessary catalyst to activate
the surface during electroless Cu growth. With the aid of microcontact printing to
define the metallization patterns, Cu films can be selectively deposited. Hence, a
metal doped polyimide film that is catalytically tailored for electroless deposition offers
a much simpler method of depositing metal selectively on polyimide.
8)
Project Title
:
Cytotoxic compounds from the Coprophilous
Fungus Coprinus sp.
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Personnel
:
:
:
:
:
:
CHE-10-2-06
NSRI
2010 – P 435,718
January 1, 2010 – December 31, 2011
Dr. Florecita S. De Guzman
1 University Research Associate I
Description of the project:
Coprinus sp., the coprophilous fungus used in this project, was isolated from
horse dung. Our preliminary studies have shown that organic extracts of the liquid
cultures of this fungus showed cytotoxic activity using the brine shrimp lethality assay.
This project aims to isolate and identify the cytotoxic component(s) of Coprinus sp.
Isolated from horse dung.
The secondary metabolites could be useful for
pharmaceutical applications
9)
Project Title
:
Alpha Glucosidase Inhibitory Compounds from
Terminalia catappa Leaves
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Personnel
:
:
:
:
:
:
CHE-10-2-07
NSRI
2010 – P 449,718
January 1, 2010 – December 31, 2011
Dr. Evangeline C. Amor
1 University Research Associate I
Description of the project:
The International Diabetes Federation reported that in 2007, 246 million of the
world’s population is afflicted with diabetes and this is seen to increase alarmingly in
2025. Diabetes mellitus is among the top ten causes of mortality in the Philippines. In
2030, the Philippines is expected to be among the top ten countries with the highest
prevalence of diabetes. Hence, there is an urgent need to address this issue.
19
Glucose from the diet enters the bloodstream through the small intestines. A
major enzyme that plays a key role in this is alpha-glucosidase.
Inhibition of this
enzyme is one of the mechanism by which blood glucose levels can be controlled
especially in Type II (NIDDM) diabetes.
Natural products remain to be recognized as a significant source of lead
compounds for drug development for the purpose of managing, treating and curing
various ailments that afflict the world population. And, plants provide a rich source of
natural products. There remains to be a significant number of medicinal plants that
remain untapped as sources of bioactive compounds as reported by NAPRAIert. One
such plant is Terminalia catappa, whose leaf extract has been reported to exhibit
significant alpha-glucosidase inhibitory activity. It is the objective of this project to
isolate and elucidate the structure of the alpha-glucosidase inhibitory compounds from
the leaves of T. catappa.
MATHEMATICS
10)
Project Title
:
MGOPT: Implementation and Analysis of Multigrid
for Solving Elliptic: Optimal Control Problem
with Constraints
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
:
MAT-10-1-01
NSRI
2010 – P 116,000
January 1, 2010 – December 31, 2010
Dr. Michele Vallejos
Description of the project:
The significance of this research is to develop a fast numerical solver for solving
optimal control problems. This research will be an extension of the application of
multigrid to optimal control problems, this time with box constraints. Based on certified
reference materials, not only the application but also the analysis of the said
optimization method will be discussed.
11)
Project Title
:
Times Series and Image Analysis Using Persistent
Homology
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Co-Project Leader
Collaborators
:
:
:
:
:
:
:
MAT-10-1-02
NSRI
2010 – P 160,400
January 1, 2010 – December 31, 2010
Dr. Maria Vivian V. Visaya
Mr. Alva Benedict C. Balbuena
Dr. Job Nable
Mr. Raymond Melton
Description of the project:
Standard methods of statistics are often used in analyzing data sets. Methods
used in image analysis for instance, are mostly based on applying geometry and
statistics. However, powerful tools in algebraic topology have emerged for studying
not just the quantitative features but the structure of the mathematical object
captured by large sets of either numerical or physical data, or even images. There
have been various means in giving a qualitative and quantitative description in
describing and recovering as much information of the underlying object by statistical
techniques but nonlinear problems seem to demand a topological solution. Another
20
issue that we would like to address is a definition of randomness of a data set, a term
which has not been formally defined in statistics.
As most systems observed in practice are highly non-linear or high-dimensional,
they become quite difficult to analyze using most standard statistical tools. As such,
the kind of tools we shall use are in part topological, in the sense that they measure
more qualitative properties of the spaces involved.
We particularly show how
algebraic topology addresses the challenge of algorithmically managing large, highdimensional data, or even non-Euclidean data. Recent applications are in image
processing, sensors, networks, neuroscience data and biological data, to name a
few.
12)
Project Title
:
On Compositions of Graphs
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Co-Project Leader
Collaborators
:
:
:
:
:
:
MAT-10-1-03
NSRI
2010 – P 140,400
January 1, 2010 – December 31, 2010
Dr. Evelyn L. Tan
Mr. Joseph Ray Clarence G. Damasco
Mr. Clarisson Rizzie P. Canlubo
Mr. Ariel R. Paningbatan
Description of the project:
A graph G is an ordered pair (V(G),E(G)) where V(G) is a finite nonempty set
whose elements are called vertices and E(G) is a set of unordered pairs xy of distinct
elements x,y of V(G) called edges.
If xy is an edge in G, we call x and y its
endvertices. A graph H is a subgraph of G if V(H) is a subset of V(G) and E(H) is a
subset of E(G). An xy-path in G is a nonempty sequence of distinct vertices x=v0,
v1,…..vn-1,vn=y such that v1vi+1, i=0,l,...,n-1, is an edge in G. A graph is connected if for
every vertices x and y, there exists an xy-path in G. Let S be a nonempty subset of V(G).
The subgraph induced by S, denoted by [S], is the subgraph of G whose vertices are
those in S and whose edges are those in E(G) having both endvertices in S. A
composition of a graph G is a partition of V(G) such that each set (called part) in the
partition induces a connected subgraph of G.
Knopfmacher and Mays [1] introduced the idea of a composition of a graph G.
This idea generalizes the concept of partitions of n-sets and of composition of integers,
[1], [4], as these are analogous to the composition of the complete graph Kn and that
of the path Pn, respectively. The number of compositions of a graph G is denoted by
C(G). In [1], the number of compositions of trees, complete graphs, cycles, wheels,
ladders, complete bipartite graphs and graphs obtained from Kn by deletion of one
edge were studied.
ENVIRONMENTAL SCIENCE AND METEOROLOGY
13)
Project Title
:
GIS and Remote Sensing Techniques to Estimate
Stormwater Pollutant Loadings
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
:
ESM-10-1-01
NSRI
2010 – P419,718
January 1, 2010 – December 31, 2010
Dr. Lourdes V. Abellera (Jan. 1, 2010 – May 31, 2010)
Dr. Tolentino B. Moya (June 1, 2010 – Dec. 31, 2010)
21
Collaborators
:
Personnel
:
Dr. Michael L. Stenstrom
Dr. Anand V. Panangadan
1 University Research Associate I
Description of the project:
Stormwater is a considerable source of non-point pollution as it contains
numerous toxic pollutants. Stormwater runoff can convey sediment, nutrients, organics,
noxious substances, and pathogens to drainage systems and large water bodies.
Identification of land areas with high stormwater pollutant emissions will enable policy
makers to prioritize particular areas in the watershed for the implementation of best
management practices (BMPs). The volumes and kinds of pollutants produced are
strongly correlated with land use/land cover. Different types of land cover have
distinctive spectral signatures which make them identifiable from satellite images. In
addition, many studies argue that the amount of impervious surface in a land parcel is
directly proportional to the amount of contaminants produced. The goal of this
investigation is to derive an empirical relationship among rainfall, runoff, and
impervious surface. From this relationship, we can compute for the pollutant loadings
of selected water quality parameters. Eleven water quality parameters are specific to
urban non-point source runoff and will be modeled in this investigation. They are total
suspended solids, biochemical oxygen demand, chemical oxygen demand, nitrite and
nitrate, tota Kjedahl nitrogen, total phosphorus, soluble phosphorus, total copper, total
lead, total zinc, and total grease. Statistics, remote sensing, and GIS technique
will be applied in this study.
14)
Project Title
:
Sea Surface Temperature Variability During High
Sealevel Stands Recorded by Porites Corals from
Bohol, Philippines
Project Code
Funding Source
Amount of Funding
Duration of Project
Project Leader
Collaborators
Personnel
:
:
:
:
:
:
:
ESM-10-1-02
NSRI
2010 – P 455,718
January 1, 2010 – December 31, 2010
Dr. Cherry L. Ringor
Dr. Chelo S. Pascua
1 University Research Associate I
Description of the project:
Climate involves interactions among the atmosphere, the oceans, the land
surface as well as its vegetation and hydrology, and naturally varies on time scales
ranging from interannual to millennia or longer. The instrumental record of a hundred
years or so is clearly inadequate in providing a better understanding of these processes
and emphasizes the need for knowledge of the long-term behavior of these systems.
Thus, records extending beyond thousands of years are necessary to establish natural
baseline of variability against which future anthropogenic-induced changes in climate
regimes can be evaluated.
The Last Interglacial, ~120,000 years ago, represents the last time global sea
levels were at or near modern sea levels, and by inference, the last time ice volumes
and global climatic conditions were similar to present day.
Information on
paleothermometry during this period, therefore, has direct implications on the present
stability of major ice sheets which is crucial for future assessment of global climate.
Although the long warm episode about 400,000 years ago represents an even closer
analog to the current interglacial stage, the relative lack of paleoclimatic evidence
from that period makes the Last Interglacial as the best available interval to study the
processes and changes during warm periods.
22
In inter-annual scale, one of the coupled ocean-atmosphere interactions that
principally modulates global climate is rooted in the tropical Pacific Ocean. The
western equatorial Pacific warm pool is believed to be the source region of a
substantial proportion of the world’s inter-annual climate variability, including the
globally significant El Niño Southern Oscillation. It is a region of particularly high sea
surface temperatures, intense precipitation, and the greatest latent heat transfer to the
atmosphere of any open ocean area on earth, hence it constitutes an important
component of the climate system. In spite of this, the response of the tropical oceans
during glacial to interglacial transitions remains uncertain. The Philippine archipelago is
situated within this so-called warm pool, hence corals that grew within its surrounding
waters serve as sensitive recorders of mild as well as severe climatic events. Despite of
this strategic location, the Philippines remains poorly served by proxy climate records
extending beyond the past few decades.
This research aims to extract paleothermometric records from Porites corals that
grew 120,000 years ago in Bohol, Philippines. The availability of corals representing the
Last Interglacial highstands in the said area provides a record of temperature
fluctuations, at an interval of about 25,000 years, at the time when sea levels were
high. Moreover, the study area is ideally located within the inner waters of central
Philippines which are directly influenced by two contrasting basins, the marginal Sulu
Sea and the open Philippine Sea, providing spatial coverage. The data will be used to
investigate the variability of paleo-sea surface temperatures (SSTs) at this tropical site
during the Last Interglacial highstands. The acquisition of these data are valuable
since more accurate estimates of the past variation of SSTs in tropical surface waters is
crucial to establishing the role of the tropical climate in global climate change.
23
EXTERNALLY – FUNDED PROJECTS
(NSRI regular research staff received grants for the following externally funded projects)
1)
Project Title
:
Feasibility of Recovering Ancient DNA Sequences in
Philippine Bioarchaeological Remains
(A collaborative research between the BRSL and the UP
Diliman Archaeological Studies Program)
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVCRD
P 1,683,600
October 1, 2008 - September 30, 2010
Dr. Philip J. Piper
Status of the project:
Molecular genetics and ancient DNA research promise to revolutionise how
archaeologists study the past. In recent times, the field of Archaeogenetics has vastly
added in our understanding on the human colonisation process in Southeast Asia and
this study provides vital insights into the mechanisms of this colonisation process. In this
study, the phylogenetic relationship of 23 pigs from the Philippines was assessed
together with 387 GenBank sequences using 666 bp of mitochondrial DNA (mtDNA) Dloop sequences. The phylogenetic analysis of the constructed phylogenetic tree
indicated that pigs in the Philippines traces its ancestry with pigs found in East Asia.
Furthermore, the phylogenetic association of five pigs in the study with Lanyu pigs
suggests that these pigs were introduced into the Philippines by way of Taiwan
providing insights on the translocation of pigs as one of the suite of the Neolithic
package as exemplified by the Out-of-Taiwan hypothesis. Moreover, to add timedepth into the analysis, four archaeological bone and one ethnographic bone
samples were processed for ancient DNA. The pig aDNA sequences did not produce
enough character- state information which is sufficient enough for a temporal insight to
be made. This attempt, however, has shown that aDNA material may be isolated from
tropical archaeological bones even from a sample with age up to 1500 years old.
2)
Project Title
:
An Archaeobiomolecular Study of Lipid Residues in
Ceramics
(A collaborative research between the BRSL and the UP
Diliman Archaeological Studies Program)
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVPAA
P 207,000 less 20%
October 1, 2009 - September 30, 2010
Dr. Philip J. Piper
Description of the project:
How and when people started exploiting various animal and plant resources is
crucial to our understanding of past human subsistence, the introduction of domestic
animals and plants, and the earliest production of food products such as rice cakes
and fermented fish/shrimp in the Philippines. Although the recovery of animal and
plant remains from archaeological sites can provide some direct or indirect evidence
for the utilization of many resources, others such as oils used for cooking or as fuel in
lamps are elusive by the conventional identification of animal bone and plant remains
from archaeological sites. The chemical analysis of organic residues from ceramic
cooking and storage vessels, on the other hand, provides direct evidence for the
processing and consumption of animal and plant products. This research is an initiative
to establish organic residue analysis as a standard archaeological procedure in the
Philippines through the initial production of reference materials, testing of an analytical
procedure for extraction and detection of lipid residues, and to test its application on a
24
chosen assemblage of archaeological materials. Firstly, modern comparative
reference materials were prepared by cooking selected foodstuff in pots. Extraction
and analytical procedures were then tested on them to assess and optimise the
recovery of residues. Fatty acids and other lipid components were successfully
recovered and detected with gas chromatography analysis from these reference
materials. The procedure was then applied to the absorbed organic residues in
earthenware pots excavated from a 13th-14th century settlement site in Porac,
Pampanga. Fatty acids and other organic components that are still preserved were
also successfully recovered and detected from these ancient pots. Gas
chromatography analysis showed that the reference materials were useful in
determining the possible contents of the archaeological pots. In conjunction with the
available bioarchaeological data, the results gave insights on, at least, the kinds of
food eaten by the people living in Porac, Pampanga during the 13th-14th centuries.
This work demonstrated that the organic residue analysis will be a viable procedure for
Philippine archaeology in the future, despite being laden with challenges.
3)
Project Title
:
Forensic DNA Analysis of Vaginal Swab Samples from
Child Victims of Sexual Assault Using Y-STR Multiplex
Typing Procedure
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVCRD
P 300,000
August 2009 – August 2010
Ms. Minerva S. Sagum
Description of the project:
The laboratory evaluated 154 sexual assault cases from four Child Protection
Units in the Philippines involving female child victims aged from 2 years to 18 years old.
All child victims sought medical attention within 72 h after sexual contact. In 130 cases,
the child victim knew the alleged offender and identified him during the interview with
the social worker. Penile ejaculation was reported by 68 child victims with varying
reports of washing after contact. Overall, 84 child victims admitted having wiped their
genitalia prior to the collection of biological samples for DNA testing. Laboratory
personnel examined vaginal smears in only 109 cases using a light microscope and
reported 23 samples to be positive for sperm cells. Using the PowerPlex® short tandem
repeat of the Y chromosome (Y-STR) DNA multiplex system, male DNA was detected in
vaginal swab samples from 63 child victims. In 39 cases, positive amplification at 11 YSTR DNA markers consistent with a single male DNA profile was observed.
4)
Project Title
:
Validation of Procedures in DNA Extraction from
Formalin-Fixed Paraffin-Embedded Tissues (FFPET)
Samples
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVCRD
P 300,000
approved in 2009 but not yet started
Ms. Gayvelline C. Calacal
Description of the project:
DNA analysis is a powerful tool for forensic human identification purposes as
each individual possesses a unique genetic profile with the exception of identical twins.
Any biological sample i.e. blood, bone, tooth, tissue, can be used to generate a DNA
profile. In forensic applications, biopsy tissues can be used as a source of genetic
information for personal identification or paternity testing when no other specimen is
available. Moreover, the DNA technology can also be used to establish identity or
verify the true tissue sample source in issues of specimen mix-up or identity fraud.
25
We proposed to study different DNA extraction techniques to increase
efficiency of recovering and isolating
DNA from formalin
fixed
paraffin
embedded
tissue (FFPET) and
subsequently, analysis of
nuclear and
mitochondrial DNA to generate a genetic profile. DNA typing results (nuclear
DNA profile and mtDNA sequence) obtained from the FFPET will be evaluated
before application in Philippine casework.
5)
Project Title
:
Validation of Extraction Methods for Human DNA from
Used Cigarettes.
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVCRD
P 300,000
approved in 2009 but not yet started
Dr. Maria Corazon A. De Ungria
Description of the project:
DNA analysis of forensic evidentiary material is a powerful tool in linking an
individual to the victim or the crime scene. Saliva may be found in various surfaces
found in the crime scene such as used cigarettes and leftover food. By extracting DNA
from the epithelial cells present in saliva, these pieces of evidence can be used for
DNA analysis.
However, saliva stains are invisible to the naked eye when dry and only contain
minute amounts of DNA thus emphasizing the need for an effective extraction method.
This study aims to validate an extraction protocol for DNA from cigarette butts that
would be best suited for the laboratory’s capabilities and purposes.
6)
Project Title
:
DNA Testing of Child Trafficking Cases
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVCRD
NA
2009 – 2011
Dr. Maria Corazon A. De Ungria
Description of the project:
DNA Pro-kids is an international humanitarian initiative aimed at fighting against
traffic in human beings within and across national borders using genetic identification
of victims and their families, especially children (www.dna-prokids.org). Trafficking of
children in the Philippines for prostitution, illegal adoption, sale of organs, child labor
and other forms of exploitation is significant, hence, the passage of Republic Act 9208
or the ‘Anti-Trafficking in Persons Act of 2003’. This law also created the Inter-Agency
Coalition Against Child Trafficking (IACAT) chaired by the Secretary of the Department
of Justice (DOJ) and the Department of Social Welfare and Development (DSWD),
which was mandated to coordinate all efforts to fight trafficking in the Philippines.
However, only 13 traffickers have been prosecuted after six years since the law was
passed. One of the weakest links is in the unavailability of evidence to identify women
and children who have been trafficked; and their ‘traffickers’.
In November 2008, the DNA Analysis Laboratory of the Natural Sciences
Research Institute of the University of the Philippines Diliman campus (UPNSRI) was invited
to join the initiative and to function as the reference
laboratory
that would coordinate local efforts to develop
forensic DNA
technology for this purpose. In October 2009, the University of the Philippines
Diliman signed a Memorandum of Agreement with the University of Granada,
26
which signified the University’s commitment to share its technical resources and
facility for the conduct of research, training and extension work in line with the
implementation of the DNA Pro-kids program in the Philippines.
7)
Project Title
:
Evaluation of Real-Time PCR Assays for Quantitation of
Nuclear and Mitochondrial DNA: Application to
Forensic DNA Analysis
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVCRD
P 300,000
January 2009 – July 2010
Ms. Jazelyn M. Salvador
Description of the project:
Quantitation of DNA using real-time quantitative PCR (qPCR) has generated a
great deal of interest in the forensic DNA typing community in the past several years.
With the recent development and improvement of DNA typing technologies for
human identification (e.g. use of commercial multiplex Short Tandem Repeat (STR)
assays, DNA sequencing, and high throughput and sensitive analysis platforms), careful
evaluation of the quantity and quality of DNA extracted from biological samples is an
essential step. Incorporation of a more sensitive DNA detection system such as qPCR
prior to STR typing in the DNA analysis procedure is a more cost-effective way of
analyzing biological evidence. For example, several biological samples are often
submitted in a forensic investigation, but in most cases, only a few contain amplifiable
DNA. Thus, qPCR significantly improves the success rate of generating a DNA profile
from an evidentiary material and significantly reduces DNA typing cost by analyzing
the appropriate samples for STR typing. In addition, quantitation is often crucial as
samples may be limited - particularly from environmentally-compromised and highly
degraded samples that are often encountered in mass disaster incidents and from
crime scene investigations e.g. biological stains, body fluids.
Notably, DNA
quantitation assays such as slot blot technology that have been employed at the UPNSRI DNA analysis laboratory in the past, are often less than accurate and sensitive, are
time consuming, and require the consumption of a substantial amount of precious
biological samples.
In order to establish a more sensitive, accurate and precise method in assessing
the quantity and quality of human DNA in forensic casework samples, three qPCR
assays were evaluated and assessed as to their applicability in handling
environmentally-compromised samples and DNA mixture samples collected from
victims of sexual assault.
8)
Title of Project
Funding Source
:
Project 2 – Marine Microorganisms: Characterization
and Culture Optimization under the Program, “Marine
Drug Discovery from Sponge-Associated
Microorganisms and from Turrid Snails for Anti-Pain
Agents (PHILIPPINE PHARMASEAS DRUG DISCOVERY
PROGRAM/PHARMASEAS)
:
Philippine Council for Aquatic and Marine Research
and Development (PCAMRD), Department of
Science and Technology (DOST)
Year 2: January 1, 2010 to April 14, 2010 – P
830,940.60
Year 3: April 14, 2010 to December 31, 2011 – P
1,296,507.58
Year 2: January 1, 2010 to April 14, 2010
Year 3: April 14, 2010 to December 31, 2011
Amount of Funding
:
Duration of the Project
:
27
Project Leader
:
Dr. Maria Auxilia T. Siringan
Abstract:
Project 2 of the PharamaSeas Program is one of the major projects in the
Program, “Marine Drug Discovery from Sponge-Associated Microorganisms and from
Turrid Snails for Anti-Pain Agents or PHILIPPINE PHARMASEAS DRUG DISCOVERY
PROGRAM (PHARMASEAS).
Project 2 is involved with two major tasks:
a)
characterization of priority marine microorganisms (MMOs) isolated and screened by
Project 3 and b) culture optimization involving the determination of key physicochemical and nutritional requirements, such as carbon and nitrogen source, for the
optimum growth and anti-infective production of priority MMOs.
Phenotypic characterization studies were performed on four Year 2 (Y2) priority
MMOs, namely, ISP2-576-A, R2A-380-A (380-A), R2A-433-A (433-A) and SDA-15-A (15-A).
The priority MMOs exhibited activity against Pseudomonas aeruginosa ATCC 10145,
Staphyloccus aureus ATCC 12600, Mycobacterium tuberculosis and the influenza A
(H3N2) Hong Kong/8/68 virus ATCC VR-544. Definitive species determination was done
only in isolate SDA-15-A, which was identified as Pseudomonas aeruginosa by both API
20NE and Biolog identification systems. Further characterization of isolates ISP2-576-A,
R2A-380-A (380-A) and R2A-433-A (433-A), will be performed using appropriate
phenotypic tests.
Identification of isolate 433-A, previously determined as an
actinomycete using both API and Biolog ID systems was not performed since these
systems do not include actinomycetes in their database and hence, are not
applicable to the isolate. For the fungal isolate ISP2-576-A (576), Biolog FF ID system
generated an inconclusive identification, with Penicillium ratistrickii as the closest
candidate. However, Project 5 earlier reported (Y2 Q4 annual report) that the fungus
belongs to P. griseofulvum, based on 18S rDNA analysis. Characterization of both
isolates 380-A and 576 using the Biolog system will be repeated with appropriate
growth media, correct inoculating fluids and new Biolog plates to ascertain their
taxonomic ranks.
Confirmation of taxonomic ranks can be repeatedly and
systematically performed with the availability of the Biolog Microstation and databases
in the laboratory.
Study on the growth of MMO SDA-15-A showed that the organism entered the
logarithmic stage after 2 hours of incubation, yielding an optical density of 0.1 and a
viable cell count of 1.9x108 cells/ml. Entry to the mid logarithmic phase occurred on the
6th hour, with an absorbance 0.42 and a viable cell count of 3.44 x 108 cells/ml.
Stationary phase was attained at the 10th hour, yielding an optical density of 0.52 and
a viable cell count of 1.12x109 cells/ml. The end-point reading at the 24th hour yielded
an optical density of 0.45 and viable cell count of 2.36x 109 cells/ml. The growth curve
data would be used as reference timetable for harvesting the target metabolites
produced by isolate 15-A in future upscale productions.
Data on pH and salt requirements of three MMOs namely, ISP2-1-A, ISP2-23-A2,
ISP2-602-A were generated. The following are the pH levels and salt concentrations
which supported the growth of the three MMOs: a) for ISP2-1-A - pH 5 to 9 and 2%, 3%,
and 5% NaCl; b) for ISP2-23-A2 – pH 5 to 8 and 2% to 6% NaCl; and c) for ISP2-602-A –
pH 8 to 9 and 2% NaCl. The three MMOs were able to tolerate pH 4 to10 and 0% to 9%
NaCl except for ISP2-602-A, which tolerated up to 10% NaCl concentration. The data
could be used as a guide in the optimization of the physico-chemical conditions that
will enhance the growth of the isolates.
Culture optimization studies for isolate ISP2-569-A used three media, namely
ISP4, MMD, and MM, to assess the growth and metabolite production of the isolate at
day 7 and day 14. MMD yielded the highest cell levels on the 7th day while on the 14th
day, ISP4 exhibited the highest cell count based on absorbance values. Although ISP4
appeared to be the best culture medium for growth, assessment of target metabolite
production through HPLC analysis reported otherwise. MMD gave the highest
metabolite yield on the 7th day of incubation. On the other hand, significant
28
metabolite production was only observed on day 14 in cultures grown in ISP4 and MM.
Based on the results of these studies, MMD is likely the best candidate medium for the
production of the target metabolite due to three reasons: a) isolate 569 gave a high
yield of the target metabolite at a short period of time (day 7); b) MMD supports
relatively moderate cell levels; and c) MMD is a simple medium comprising of relatively
inexpensive components.
Early production of the anti-infective is desired in order to make the production
cost-effective. Anti-infective production will be more favored when the isolate utilizes
its energy for secondary metabolite production instead of supporting growth. The
medium, as exemplified by MMD, should support sufficient but not copious cell
biomass.
Moreover, MMD consists of readily available components and is easy to
prepare, thus, it will be more practical and economical to use this as culture medium
for large-scale production of the target anti-infective.
Glucose, maltose, and starch were the carbon sources that favored the growth
of isolate R3 based on the results reported in Y2 annual report. Cultures grown in media
with one of these carbon sources yielded higher absorbance readings than the other
carbon source-amended media used. In the previous carbon source requirement
studies using a microtiter format, maltose appeared to be the best carbon source. In
this present upscaling of the microtiter plate format-based experiments, using 50 ml
R2A, glucose was shown to be the best carbon source for the growth of isolate R3.
Previous study showed that monosaccharides, such as glucose, can inhibit the
synthesis of secondary metabolites via carbohydrate catabolite repression (Ruiz et al.
(2010). It must be noted that a nutrient that can enhance the growth of the bioactive
microorganism does not necessarily enhance the production of its metabolite. Thus,
bioassay-guided culture optimization studies are currently conducted in order to
determine the carbon source and other nutrients that can improve the anti-infective
produced by the priority MMOs. The use or non-use of glucose, maltose and starch as
carbon sources for isolate R3 is currently being confirmed in on-going bioassay-guided
optimization experiments.
Currently, HPLC analyses of extracts derived from R3
cultures grown with glucose, maltose starch as sole carbon source are in progress.
Results of these analyses will facilitate the selection of the carbon source that can
increase the yield of the anti-infective metabolite produced by isolate R3.
The design of the optimization studies will be aided by SYSTAT, a software used
in the Response Surface Method (RSM). RSM is an approach that allows the
establishment of set of conditions for optimum growth and subsequent enhanced antiinfective production.
Status of the project:
The PHARMASEAS Program which included Project 2, was renewed in May 2010.
DOST approved the Revised Line-Item-Budget for all the projects. Project 2’s LIB
included additional Equipment Outlay for one unit of a UV-vis spectrophotometer and
one unit of a refrigerated shaker-incubator. The said equipment, which were part of
the Year 2 LIB, were considered for the Year 3 LIB. With the approval of the realigned
Year 3 LIB, funds for the MOOE were reduced in order to increase the Personnel
Services funding. Additional manpower was foreseen as a priority due to the extent
of bioassays that will be performed for the optimization studies. In this connection, Ms.
Maria Arlin A. Laguitan, a licensed chemist, was hired from September to December
2010 as a member of Project 2’s research team. Ms. Laguitan’s contract of service was
renewed for the period January 01 to April 14, 2011. Year 3 of Project 2 started on April
15, 2010 with full funding from PCAMRD-DOST.
29
9)
Project Title
:
Global Air Passive Sampling
Funding Source
:
Environment Canada Air Quality Process Research
Division for Global Air Passive Sampling (GAPS) Project
Amount of Funding
:
Duration of Project
Project Leader
:
:
P 10,000/year (Collaboration in sampling
expenses for sampling materials and shipping
provided by Environment Canada. Transportation
and other expenses in deploying and retrieving the
samples are shouldered by RASL Trust Account)
January 2005 – on-going
Dr. Evangeline C. Santiago
Abstract:
The Global Air Passive Sampling Project is organized to monitor the Persistent
Organic Pollutants in strategic places around the globe to generate baseline data on
the concentrations of Polychlorinated Biphenyls and Organochlorine Pesticides that
are covered by the Stockholm Convention. The data will be used as reference to
assess the effectiveness of the Convention.
Fig. 1. Passive Air Sampler used for the Global Air Passive Sampling (GAPS) Project
10)
Project Title
:
Monitoring of Polybrominated Diphenyl Ether (PBDE) in
Sediments with funding from the United Nations
University
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
United Nations University
$ 10,000
August 2010 – on-going
Dr. Evangeline C. Santiago
Abstract:
The major source of PBDEs in the environment is associated with the use of these
compounds as flame retardants in plastic and textiles. It is expected that PBDEs can be
found in the environment of highly urbanized and industrial areas where plastics and
fabrics are applied for different purposes. It is also expected that waste recycling
activities for plastic materials will contribute to the pollution of the environment.
The sampling sites for monitoring in the Philippines for the year 2010 were four
major different river tributaries of Manila Bay and two river tributaries of Laguna Lake.
The purpose of the study is to compare the PBDE concentrations in the sediments in the
different areas in the Greater Manila Area and its neighboring provinces.
Among the sediment samples investigated, only the samples from Tenejeros,
Malabon and Looc, Calamba City showed concentrations that exceeded the LOQ
(2.529 ng/g dry wt) for Deca-BDE(BDE-209) at 4.855 ng/g dry wt, 13% RSD and 2.678
ng/g dry wt, 38% RSD respectively. The rest of the samples except the sample from
Calumpit showed detection of Deca-BDE (BDE-209) at concentrations above the MDL
(0.759 ng/g dry wt) with acceptable % RSD. Aside from Deca BDE, Tri BDE (BDE-028)
was detected above MDL (0.110 ng/g dry wt) but below LOQ (0.365 ng/g dry wt) in
Tullahan (0.139 ng/g dry wt, 28% RSD) and in Looc, Calamba City (0.235 ng/g, 7% RSD).
Hexa BDE (BDE-154) was also detected above the MDL (0.834 ng/g dry wt) but below
the LOQ (2.781ng/g dry wt) at 0.881 ng/g dry wt, 6 % RSD in Tenejeros, Malabon.
Given the instability of the adopted procedure, the sediment samples showed
contamination of Deca-BDE at varying levels with acceptable confidence. The rest of
the PBDEs could be detected more accurately with an alternative method where the
stability of the PBDEs during the clean up process could be assured.
Fig. 2. Collection of sediment
samples for analysis during
sampling in Pasig River Area
11)
Project Title
:
Conduct of Sampling for the PhD Dissertation
Research on the Fate and Distribution of Brominated
Flame Retardants in Tropical Asia
Funding Source
:
Amount of Funding
:
Duration of Project
Project Researcher
:
:
JSPS RONPAKU (Dissertation PhD) Program
UP NSRI RASL (for transportation and laboratory space
during sampling)
Travel and accommodation allowance for the
Japanese
Advisor and actual transportation cost during
sampling
October – December 2010
Ms. Charita S. Kwan (Advisee)
Prof. Hideshige Takada (Japanese Advisor)
Dr. Evangeline C. Santiago (Home Advisor)
Abstract:
For the year 2010, the focus of the sampling activities was source identification
of PBDEs in the Philippine Coastal Environment. Water and sediments from 2 esteros
and four tributary rivers to the Pasig River were collected. Leachate samples from the
Quezon City Controlled Disposal Facility (Payatas Dumpsite) were also collected. The
liquid samples, water and leachates were each filtered through a pre-weighed 90 mm
glass fiber filter paper to trap the PBDE adsorbed fraction. The dissolved PBDE fraction
in the filtrate was also trapped through a C-18 SPE cartridge. The sediment samples
were stored in an Al container. All samples were brought to Japan and will be
analyzed for the levels of PBDEs at the Laboratory of Organic Geochemisty, Tokyo
University of Agriculture and Technology.
12)
The 2010 DA-BAR/UP-NSRI Research Project on “Post-Doctoral and Senior Scientist
Research Fellowship in Basic Research for Agriculture and Fisheries”
Project Title
:
Influence of Roasting on the Chemical Attributes and
Antioxidant Activity of Philippine Coffee
Funding Source
Amount of Funding
Duration of Project
Project Leader
Host Scientist
Project Dev’t Associate
:
:
:
:
:
:
DA-BAR
P 500,000
June 15, 2010 to June 14, 2011
Dr. Ruel M. Mojica
Dr. Leni L. Quirit
Mr. Jay Mathias A. Arellano
Description of the project:
The project aims to investigate the influence of roasting on the antioxidant
properties of coffee samples using different antioxidant activity methods. The
antioxidant level of two Philippine coffee varieties will be investigated by measuring its
total phenolic content. Antioxidant activities will also be measured using radical
scavenging capacity and reducing power as parameters. The data that can be
obtained from the project will provide firsthand information on the phenolic
content and antioxidant activity of two Philippine coffee varieties thereby promoting
the products in the world market.
32
NSRI-ASSISTED RESEARCHES
(NSRI provided laboratory space, electrical and water facilities for the following assisted researches of
the faculty of the Institute of Biology, Institute of Chemistry and the National Institute of Physics)
1)
Project Title
:
Molecular Characterization of Acanthamoeba spp.
Isolated from Contact Lens Storage Cases, Soil and
Water
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
OVCRD
Php 300,000
April 16, 2007 – April 15, 2010
Dr. Windell L. Rivera
Status of the project:
Free-living amebae of the genus Acanthamoeba are ubiquitous in nature and
are found in diverse habitats such as soil and water supplies. Several species of
Acanthamoeba have been associated with serious human diseases, including vision
threatening amebic keratitis (AK), especially in contact lens wearers and fatal
granulomatous amebic encephalitis (GAE) in immunocompromised patients. Prior to
this project, there was no study on the occurrence of Acanthamoeba contamination
in contact lens storage cases in the Philippines. Moreover, molecular identification of
the existing genotypes of Acanthamoebae from different sources based on its 18S
rDNA has not yet been studied. This research project therefore evaluated the genetic
diversity of Philippine Acanthamoeba isolates at the molecular level through rRNA
gene fragment sequences.
2)
Project Title
:
Bioassay Guided Isolation of Hypoglycemic
Compounds from Philippine Medicinal Plants using
Novel Biosensors for Multiple Metabolites
Funding Source
:
Amount of Funding
Duration of Project
Project Leader
:
:
:
National Institutes of Health-FIRCA through a
subaward from the University of Maryland Baltimore
County
US$ 72,280.48
February 2010 – November 2011
Dr. Evangeline C. Amor
Status of the project:
Ten (10) identified Philippines medicinal plants used for the treatment and/or
management of diabetes have been collected. Extraction of the appropriate plant
part has been done and the corresponding extracts will be tested for hypoglycemic
activity using the labeled glucose binding protein.
3)
Project Title
:
Transport of Low Energy Plasmas for the Synthesis of
TiCN/TiCuNfilms and Ion Treatment of Polymers
Funding Source
Amount of Funding
Amount Available for 2010
Duration of Project
Project Leader
:
:
:
:
:
DOST
Php 18,821,628.17 (programmed for three years)
Php 4,524,895,94
April 16, 2007 – May 31, 2010
Dr. Henry J. Ramos
33
Status of the project:
Project A. TiN/TiCN/TixCuyNz Coating Technology
The Sheet Plasma Negative Ion Source (SPNIS) was developed from previous
projects purposely for the production, extraction and enhancement of negative
hydrogen ions (H-) using a mixture of hydrogen, argon and magnesium plasma. The Hions extracted using a modified Wien filter was used in the synthesis of silicon hydride.
Selective solar (IR) coatings of tin-bismuth on various metal substrates were also
deposited using the facility.
In this project component, the facility is used for the synthesis of titanium nitride
(TiN), carbon nanostructures, and multilayered hard coatings of TiCN. The specific type
of cutting, engraving, milling, and punching tools used by industries in the Philippines
will be solicited for actual coating and lifetime tests. Samples of cutting tools and the
like used by industry practitioners are obtained and coated with TiN, TiCN, TiCuN or
layers thereof. The coated samples are then tested in coordination with the various
industries in actual performance tests. These tests serve as demonstrations and proof of
principle of the capability of the developed coating technology. The documented
tests from the various industries (the same industrial collaborators for the TiN testing)
serve as baseline data on lifetime enhancement and actual performance of these
TiCN-coated and multi-layered samples.
Project B. Polymer treatment of low energy ions from a gas discharge source
A Gas Discharge Ion Source (GDIS) is developed as an example of a low
energy ion beam source. Ion beam diagnostics like beam emittance measurement
and mass analysis are done to investigate optimum parameters in producing mixed
species hydrogen positive ions. By producing a low energy ion beam (H+ and H2+), this
source is tested for surface modification applications such as ion beam irradiation on
sample polymers. The effects on structural organic polymers such as wood, sea grass,
polytetrafluoroethylene, polyethylene and others are tried. The ion treatment that the
sample surfaces undergo changes their physicochemical properties. The modification
is of great significance in the moisture absorption of the material improving its
characteristic features like dyeability, anti-stain, and other physical characteristics.
Present results can be extended to applications on other polymers, bio-organisms and
semiconductors. Other gas ions like oxygen, helium and nitrogen are to be irradiated
on similar polymers.
Project C. Beam focus, enhancement and transport in a sputter-type source
In this study, Zirconium (Zr), Copper (Cu) and Gold (Au) are used as metal
targets to produce negative metal ions. The metal ions will be fully characterized using
in terms of ion beam energies and ion currents using a retarding-type electrostatic
energy analyzer. The behavior of ion energies and ion currents towards increasing
target voltages and differences in beam characteristics between each metal used will
be studied. The effect of the chamber pressure to the ion beam characteristics will also
be studied.
A sequence of accelerators and focusing techniques are employed in the
extraction of metal ions produced in a sputter-type ion source. Enhancement of ion
yield is tried with noble gases. The extracted and highly focused beam is studied in
terms of its transport properties specifically on techniques of increasing acceleration
voltage reaching to several keV. The ion current is expected to increase with
increasing acceleration voltage. Acceleration voltages in the order of a few keV and
a few hundred microamperes of ion current are essential in ion beam implantation and
etching applications. Ion beam etching is necessary in the preparation of materials for
various investigations such as thinning of samples for transmission electron microscopy
or for texturing surfaces in the semiconductor industry. High-energy particles (ions or
34
neutral particles) bombard the specimen in the physical process. Ion beam etching
has merits over conventional metallographic etching methods specially when etching
composite materials or material compounds. The production of highly energetic ions in
this study is the first step towards comprehensive etching tests and parameter studies to
be done on various materials.
4)
Project Title
:
Improving Fire Endurance of Wood via Plasma
Technology
Funding Source
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
PCASTRD-DOST
P 1,369,696
August 1, 2009 – July 31, 2010
Dr. Henry J. Ramos
Status of the project:
The efficacy of chemical and plasma treatments with phosphate and boric
compounds, and nitrogen as flame retardants on wood are compared in this study.
The chemical treatment will involve the conventional method of spraying the solution
over the wood surface at atmospheric condition and chemical vapor deposition in a
vacuum chamber. The plasma treatment utilizes a dielectric barrier discharge (DBD)
ionizing and decomposing the flame retardants into innocuous simple compounds. The
developed Plasma Enhanced Chemical Vapor Deposition (PECVD) machine used
previously for sterilization applications is upgraded to a DBD machine for the purpose.
Wood samples are immersed in either phosphoric acid, boric acid, hydrogen or
nitrogen plasmas or a plasma admixture of two or three compounds at various
concentrations and impregnated by the ionized chemical reactants. Chemical
changes on the wood samples are analyzed by Fourier transform infrared
spectroscopy (FTIR) while the thermal changes through thermo gravimetric analysis
(TGA). Plasma treated samples are compared with untreated, vacuum-treated and
spray-painted samples in terms of thermal stability and fire retardant properties based
on the highest onset temperatures, temperatures of maximum pyrolysis, highest residual
char percentages and comparably low total percentage loss.
5)
Project Title
:
Streaming Neutral Gas Injection (SNGI) and its
Applications in the Synthesis of Carbon Structures
Funding Source
Equipment Cost
Amount of Funding
Duration of Project
Project Leader
:
:
:
:
:
Equipment donation from Doshisha University
~ Php 600,000
~ Php 100,000 (NIP counterpart)
January 2009 – December 2010
Dr. Henry J. Ramos
Status of the project:
The system is operated in its arc and glow discharge modes in a mixture of
hydrogen and methane or acetylene to produce diamond-like, diamond and carbon
nanostructures on silicon substrates.
6)
Project Title
:
Studies on Microwave Plasma Systems
Project A
:
Adhesion Enhancements of Metal and Plastic
Surfaces with Adhesives Using Microwave-induced
Atmospheric Plasma Jets.
Funding Source
Amount of funding
:
:
IBF Elctronic GmbH & Co. KG, Darmstadt, Germany
Php 6,850,713 (cost of equipment donation)
35
Duration of Project
Project Leader
:
:
August 2008 – August 2012
Dr. Henry J. Ramos
Project B
:
Development of Microwave-Induced Plasma Jets.
Funding Source
Amount of funding
Duration of Project
Project Leader
:
:
:
:
DOST-PCASTRD
Php 1,966,423
September 2010 – September 2012
Dr. Henry J. Ramos
Status of the project:
An atmospheric plasma jet is developed from an existing microwave
waveguide system (MWS) and equipment donated by IBF Electronic, GmbH, Germany.
The plasma chamber and vacuum systems of the MWS are replaced with customized
tapered waveguide and gas nozzle system, specifically designed for enhanced
stability of the plasma jet. The jet will be characterized by appropriate diagnostics
designed for atmospheric plasmas. It is a pioneering effort in the local R&D landscape
for atmospheric plasma devices. The facility will serve as tangible bridge for industrial
prototypes of atmospheric plasmas for various applications.
36
1.2 P UBLICATION AND CREATIVE WORKS
The NSRI regularly publishes the following:
1) NSRI Annual Report. This is a compilation of abstracts of NSRI-supported researches and
includes summary of the Institute's extension services for the year covered.
2) NSRI Research and Extension Programs. A summary of NSRI-funded researches and
extension services for the year reproduced for dissemination to the public.
3) NSRI Catalogue of Cultures. This describes the culture collection holding of the
Microbiological Research and Services Laboratory and is updated every two years.
4) R & D Services. a) Brochure of NSRI Research and Technical Services
b) Catalogue of Training Courses uploaded in the NSRI website
5) Scientific Publications Based on NSRI-Funded and NSRI-Assisted Projects. The output of
NSRI supported projects are published by researchers in scientific journals. The 2010
publications based on NSRI-funded projects and other projects housed at NSRI are
shown below (Table 1).
Table 1. 2010 Publications based on NSRI researches and other projects housed at NSRI.
AUTHOR
TITLE
PUBLICATIONS
BIOLOGY
A.A. Herrera
In vivo Evaluation of the Potent
Angiosuppressive Activity of Some
Indigenous Plants from Bataan,
Philippines
Asia Life Sciences 19(1): 183190 (2010)
A.A. Herera, A.M.O. Dee
and L.A. Ipulan
Detection of Congenital Anomalies
in Mus musculus induced by Crude
Leaf Extracts of Goniothalamus
amuyon (Blanco) Merr. and Alstonia
macrophylla Wall. Ex. G. Don
Journal of Medicinal Plants
Research 4(4) pp. 327-334
(2010)
A.A. Herrea, J.B. Ramos and
L.A. Ipulan
Teratogenic Activity of Ficus odorata
(Blanco) Merr. and Baccaurea
tetrandra (Baill.) Mull. in Mice
Embryos
Philipp Agric Scientist 93(3),
pp. 255-262 (2010)
M.C. De Guzman, and E.P.
Cao
Cadmium Binding Ability of the Bluegreen Alga Hapalosiphon
welwitschii Nagel
PhiIippine Science Letters
3(1): 76-86 (2010)
A.C.Jalandoni-Buan,
A.L.A.Decena-Soliven, E.P
Cao, V.L.Barraquio and W.L.
Barraquio
Characterization and Identification
of Congo Red Decolorizing Bacteria
from Monocultures and Corsortia
Philippine Journal of Science
139(1): 71-78 (June 2010)
ISSN 0031-7683
L.M.B. Garcia
Species Composition and Lengthweight Relationship of Fishes in the
Candaba Wetland on Luzon Island,
Philippines
Journal of Applied
Ichthyology
1-3 (2010)
37
cont. of Table 1.
AUTHOR
TITLE
PUBLICATIONS
CHEMISTRY
C.L.C. Hernandez
Anti-inflammatory, anti-mutagenic,
and mutagenic evaluation of
Crateva religiosa Forst Leaf Extracts.
Asia Life Sciences Journal 19
(1): 127-139 (2010)
L.L.Quirit, F.L.Santos,
I.M.Geronimo and
J.P.Torres)
Passive Sampling of Acid Gases,
Ozone and Ammonia in an Ambient
Metro Manila Site
The Philippine Scientist, Vol. 47
(2010)
IESM
B.M. Vallejo Jr., A.B. Aloy,
P.S. Ong, A. Tamino and J.
Villasper
Spatial Patterns of Bird Diversity and
Abundance in an Urban Tropical
Landscape: The University of the
Philippines (UP) Campus
Science Diliman, 20 (1): 1-10
(2009)
B.M. Vallejo Jr., A.B. Aloy
and P.S. Ong,
The Distribution, Abundance and
Diversity of Birds in Manila’s Last
Green Spaces
Journal of Landscape and
Urban Planning, 89: 75-85
(2009)
B.M. Vallejo Jr. and A.B.
Aloy
Estimating the Rarity of Birds and its
Ecological Context in the UP Diliman
Campus
Philippine Science Letters,
1(1): 32-36 (2009)
NSRI (In-House)
S.M.B. Maiguilla, J.M.
Salvador, G.C. Calacal,
F.C.Delfin, K.A. Tabbada,
H.B. Perdigon, M.R.M. Dalet,
M.C.A. De Ungria
Expansion of the Philippine
Autosomal Short Tandem Repeat
Population Database for DNA –
based Paternity Testing
Philippine Journal of Science
139(2) (in press)
S.M.B. Maiquilla, J.M.
Salvador, G.C. Calacal, M.S.
Sagum, S.A.E.L. Franco, F.C.
Delfin, K.A. Tabbada, H.B.
Perdigon, M.R.M. Dalet, B.J.
Madrid, M.P. Tan and
M.C.A. De Ungria
Y-STR DNA Analysis of 154 Female
Child Sexual Assult Cases in the
Philippines
International Journal of Legal
Medicine (accepted: Nov. 18,
2010)
M.C.A. De Ungria and J.M.
Jose
Forensic DNA Profiling and
Databasing: The Philippine
Experience
(Book Chapter)
Genetic Suspects: Global
Governance of Forensic DNA
Profiling and Databasing.
Edited by Richard Hindmarsh
and Barbara Prainsack.
Cambrige University Press,
Cambridge UK, pp 309-340
38
2. EXTENSION SERVICES
The NSRI’s second principal function is to render services to researchers. These services
are the following:
(1) Technical assistance and advisory services
(2) Training courses, seminars and conferences; and
(3) Information and community services
The NSRI has, however, opened these services to institutions and agencies outside UP
to maximize the use of facilities and equipment and to make the expertise of its research staff
available to the general public.
2.1 TECHNICAL ASSISTANCE AND ADVISORY SERVICES
The NSRI research and technical staff provide technical and advisory services to
researchers, students, government and private institutions as well as private individuals.
Technical Assistance
The NSRI’s technical services laboratories are organized into the following units:
A.
B.
C.
D.
Biological Research and Services Laboratory (BRSL)
DNA Analysis Laboratory (DAL)
Microbiological Research and Services Laboratory (MRSL)
Research and Analytical Services Laboratory (RASL)
The services rendered by each laboratory are found in the succeeding pages.
Socialized fees are charged by the laboratories to cover cost of supplies, equipment
maintenance, utilities and services.
BIOLOGICAL RESEARCH AND SERVICES LABORATORY (BRSL)
The Biological Research and Services Laboratory is one of the core facilities of the
Natural Sciences Research Institute of the University of the Philippines Diliman. It supports
biological services adapted to specific needs of research in the natural sciences primarily in
the evaluation of toxicity and safety of foods, drugs, and other biological substances. It is
accessible to graduate and undergraduate students, researchers, and other science
personnel from government and private institutions. Moreover, its staff conducts workshops on
the proper scientific procedures of handling laboratory mice so that researchers meet
international standards. At present, the laboratory has facilities for microscopy, cell culture
and molecular biology including processing of ancient and modern bioarchaeological
samples for population genetic studies.
Researches
Collaborative studies with the Archaeological Studies Program and the Institute of
Chemistry have been on-going:
•
Feasibility of Recovering Ancient DNA Sequences in Philippine Bioarchaeological
Remains – collaboration with UP Diliman Archaeological Studies Program with funds
from the Office of the Chancellor through the OVCRD
•
•
An Archaeobiomolecular Study of Lipid Residues in Ceramics - collaboration with UP
Diliman Archaeological Studies Program with funds from the UP System through the
OVPAA
Toxicological Assessment of Extracts of Indoor Dust, Laboratory and Faculty Room Air
Samples, and Diesel/Biodiesel Exhaust-Impacted Air using Allium cepa Assay collaboration with the Institute of Chemistry with funds from NSRI
Extension Services/Clients
The BRSL continues to support the research and undergraduate/graduate programs of
the University particularly those of the College of Science. Other clients include academic,
government and private organizations. We provide bioassay services, use of equipment and
workshops on laboratory animal handling, DNA isolation/PCR technique and plant
chromosome preparation. Data on the BRSL’s services are presented below.
BRSL Services in 2010
Fig.3. Graph of the BRSL’s services, showing that 85% of the services provided by the lab in 2010 were
through equipment rental. Other services, including workshops and assays made up the remaining 15%
of the lab’s service output.
Fig.4. Chart showing equipment rental in 2010. The laminar flow hood and analytical balance were the
most frequently used equipment, together accounting for a third of all requests for equipment use. The
rarely rented pieces of equipment are the vacuum pump, pH meter, micropipettor, sonicator, and the
electrophoretic apparatus.
Fig.5. Client type profile showing that
majority (70%) of clients who availed of the
BRSL’s services in 2010 were NSRI based
researchers (Type II); College of Science
students (Type I) represented 15%. Other UP
students and employees (III), non-UP
students and researchers (IV), and private
individuals and government agencies (V)
together comprised the remaining 15%.
There were no requests received from
multinational companies (Type VI) in 2010.
Fig.6. Client type profile of the participants
of BRSL’s workshops in 2010 showing that
non-UP researchers and students (Type IV)
made up half of the total number of
participants. UP College of Science of
students (Type I) constituted 29% while the
remaining 21% was composed of other UP
researchers and students (Type III).
Support to Students
Graduate assitance
Herrera, Michael James B.; MS Archaeology-UP Diliman. Dr. Philip Piper
(Adviser). An archaeogenetic study of the genus Sus in the Philippines and its
propositions for understanding human movements in Island Southeast Asia.
Eusebio, Michelle S.; MS Archaeology-UP Diliman, Dr. Philip Piper (Adviser).
Analysis of fatty acid residues in earthenware pottery from 13th-14th Century,
Porac, Pampanga, Philippines.
Macusi, Erna S.; PhD in Development Research – Center for Development
Research (ZEF), University of Bonn, Germany. Dr. Manfred Denich (Tutor), Prof.
Nestor Altoveros (External Supervisor; UPLB). Spatial analysis of the genetic
diversity of bittergourd (Momordica charantia L.) and its conservation in
Northern Philippines.
Analyses for thesis/special projects
Cytotoxicity assay – 7/16 (College of Engineering, UP Diliman; Bucal National
Science High School, Cavite; Bayugan National
Comprehensive High School, Agusan del Sur)
Genotoxocity assay – 5/6 (Adamson University, Manila; Bucal National Science
High School, Cavite)
Linkages
Local
U.P. Diliman Archaeological Studies Program
Trainings/Seminars conducted
Title of Training
Funding Source
Date
Trainors
Fees
No. of participants
Profile of participants
: Laboratory Animal Workshop
: Participants paid a fee for lecture and laboratory
: April 23, 2010
: Dr. Maria Amelita C. Estacio
: PhP 3,000 outsider participant, P2,500 UP student/employee
: 14
: Students, professionals
Fig.7. Participants dissecting lab mice during the hands-on session
of the Laboratory Animal Workshop on April 23, 2010.
Title of Training
Funding Source
Date
Trainors
Fees
No. of participants
Profile of participants
: Laboratory Animal Workshop
: Participants paid a fee for lecture and laboratory
: October 25, 2010
: Dr. Maria Amelita C. Estacio
: PhP 3,000 outsider participant, P2,500 UP student/employee
: 10
: Students, professionals
Fig.8. Dr. MAC Estacio showing
participants the proper techniques
used in experiments using laboratory
animals during the BRSL Laboratory
Animal Workshop on October 25,
2010.
Fig.9. Participants practice dissecting lab mice
during the Laboratory Animal Workshop on
October 25, 2010.
Recent developments and other related activities of the BRSL for 2010
•
The following items were purchased using grants from the National Science
Complex and the Philippine Bioarchaeology Initiative project:
-
-
-
-
-
-
Autoclave
Carousel shaker
Cryostat
Desiccator cabinet
Dry bath
Fluorescence/phase contrast microscope with digital camera
Hotplate stirrer
Laboratory blender
Microwave oven
Pipette controller
Portable centrifuge
Sonicator
Table-top centrifuge
Vortex mixer
Water bath
Desktop computer
Office furniture (executive desk, office chairs, stool, filing cabinet, etc.)
Manpower Development
Activities of BRSL Staff in 2010
1) RAQUEL O. RUBIO (University Researcher I)
Positions held
•
•
•
Head, BRSL
Associate Member, National Research Council of the Philippines
Member, Philippine Association for the Advancement of Science
•
•
•
•
Member, Philippine Association for Laboratory Animal Science
Member, Philippine Environmental Mutagens Society
Member, Philippine Society of Cell Biology
Member, UP Diliman College of Science Institutional Animal Care and Use
Committee
Seminars/Conferences/Symposia attended
•
•
•
•
•
9th Scientific Conference & General Assembly of the Philippine Association of
Microscopists, Inc. (MICROSPHIL). International Rice Research Institute, Los
Baños, Laguna. May 28, 2010.
2nd Annual Convention of the Philippine Society for Developmental Biology,
Inc. NISMED, UP Diliman, Quezon City. August 21, 2010.
Philippine Association for the Advancement of Science, Inc. (PhilAAS) 59th
Annual Convention. PhiVolcs Bldg., UP Diliman, Quezon City. September 9-10,
2010.
Seminar-Workshop on "Total Quality/Workplace Management (5S Program)."
NSRI, UP Diliman, Quezon City. September 17, 2010.
3rd Symposium on Long-Term Ecological and Biodiversity Research. Institute of
Biology, College of Science, UP Diliman, Quezon City. November 20, 2010.
Publications
•
Herrera, M.J.B., Eusebio, M.S., Rubio, R.O., and P.J. Piper. 2010. The Ancient DNA
Project on the First Year of the Philippine Bioarchaeology Initiative. Test Pit:
Chronicle of the UP Archaeological Studies Program. 15: 7-8.
2) ERNA LOUISE L. ELIAS (University Research Associate I)
Positions held
•
•
Member, Philippine Native Plant Conservation Society, Inc.
Member, Association of Systematic Biologists of the Philippines
Additional assignment
•
Examiner, UP College Admission Test (UPCAT) for SY 2011-2012, U.P. Diliman,
Q.C. August 7-8, 2010.
Seminars/Symposia/Trainings attended
•
•
•
•
•
Seminar on Malaysian Genetic Projects. Natural Sciences Research Institute.
June 25, 2010.
Mainstreaming Climate Change in NRM and Forest Policies and Programs in the
Philippines. DENR, Q.C. July 6, 2010.
Mainstreaming Native Species-Based Forest Restoration Conference. NISMED,
UP Diliman, Q.C. July 15-16, 2010.
Seminar-Workshop on "Total Quality/Workplace Management (5S Program)."
NSRI, UP Diliman, Q.C. September 17, 2010.
The Use of ADInstruments PowerLab Systems in Teaching and Research. Institute
of Biology, U.P. Diliman. October 26-27, 2010.
Publication
•
Roderos, R.R. and E.L. Elias. 2010. Genetic Diversity in Selected Philippine
Zingiberaceae spp. using random amplified polymorphic DNA (RAPD) markers.
The Philippine BIOTA 43:16-35.
44
3) MICHAEL JAMES B. HERRERA (University Research Associate II – OVCRD contractual)
Positions held
•
Member, Katipunan Arkeologist ng Pilipinas, Inc.
Publications
•
Herrera, M.J.B., Eusebio, M.S., Rubio, R.O., and P.J. Piper. 2010. The Ancient DNA
Project on the first year of the Philippine Bioarchaeology Initiative. Test Pit:
Chronicle of the UP Archaeological Studies Program. 15: 7-8.
4) MICHELLE S. EUSEBIO (University Research Associate II – OVCRD contractual)
Positions held
•
•
Professional Associate Member, Katipunan Arkeologist ng Pilipinas, Inc.
Member, Integrated Chemists of the Philippines, Inc.
Seminars/Symposia/Trainings attended
•
•
•
Philippine Institute of Pure and Applied Chemistry Symposium on Chemistry, Art,
and Beauty. Organized by Kapisanang Kimika ng Pilipinas and Organic
Chemistry Teachers Association. February 17, 2010.
Fundamentals of Gas Chromatography Mass Spectrometry training at
Shimadzu Philippines Corporation. Makati City. June 17-18, 2010.
Basic Gas Chromatography training at Shimadzu Philippines Corporation.
Makati City. May 20-21, 2010.
Publications
•
Herrera, M.J.B., Eusebio, M.S., Rubio, R.O., and P.J. Piper. 2010. The Ancient DNA
Project on the first year of the Philippine Bioarchaeology Initiative. Test Pit:
Chronicle of the UP Archaeological Studies Program. 15: 7-8.
Poster/Paper Presentation
•
Eusebio, M.S. Integrating Gas Chromatographic Techniques in Philippine
Archaeological Research. First Graduate Research Symposium on Analytical
Sciences. UP-Ayala Technohub, Diliman, Quezon City. February 15, 2010.
5) RHONA A. ONG (Administrative Aide IV)
Seminars/Symposia/Trainings attended
•
•
•
NSRI Safety Management Seminar. UP Diliman January 25, 2010.
Basic Microsoft Office Word & Excel. Informatics. January 29, 2010.
Seminar-Workshop on "Total Quality/Workplace Management (5S Program)."
NSRI, UP Diliman, Q.C. September 17, 2010.
DNA ANALYSIS LABORATORY (DAL)
The DNA Analysis Laboratory (UP-NSRI DAL) is one of the four research and extension
service laboratories in the Natural Sciences Research Institute of University of the Philippines,
Diliman. The UP-NSRI DAL is mainly composed of dedicated sections designed for the various
stages of DNA analysis. A secured Evidence Room provides for the cataloguing and storage of
biological samples for DNA analysis. Guests and clients are received at the UP-NSRI DAL
Meeting Room and all administrative functions are conducted at the Meeting Room.
Researches
Terminating in 2010
1. Forensic DNA Analysis of Vaginal Swab Samples from Child Victims of Sexual Assault
using Y-STR Multiplex Typing Procedurefunded by the Office of Vice Chancellor for
Research and Development, University of the Philippines, Diliman.
On-going/renewed for 2010
1. DNA Testing of Child Trafficking Cases in collaboration with the DNA-Prokids
Initiative, University of Granada, Spain and the University of North Texas Health
Science Research Center.
2. Understanding the Genetic Diversity and Identity of Filipino Populations in
collaboration with the Max Planck Institute for Evolutionary Anthropology, Leipzig,
Germany.
3. Validation of Procedures for DNA analysis of meat products funded by the Natural
Sciences Research Institute, University of the Philippines, Diliman.
Approved in 2010
1. Validation of Extraction Methods for Human DNA from Used Cigarettes funded by
the Office of Vice Chancellor for Research and Development, University of the
Philippines, Diliman.
2. Validation
of Procedures in DNA Extraction from Formalin-Fixed
ParaffinEmbedded Tissues (FFPET) Samples funded by the Office of Vice Chancellor for
Research and Development, University of the Philippines, Diliman.
3. Evaluation of real-time PCR assays for quantitation of nuclear and mitochondrial
DNA: application to forensic DNA analysis funded by the Office of Vice Chancellor
for Research and Development, University of the Philippines, Diliman
Extension Services/Clients
•
DNA-Prokids Cases
DNA services, n=20
•
DNA Paternity Testing
Inquiries, n= 416
DNA services, n= 55
Support to Students
•
Ms. Jazelyn M. Salvador is working on her Master of Science (Microbiology) thesis
under the Institute of Biology, using laboratory facilities of the UP NSRI DNA Analysis
Laboratory, the Microbiological Research Service Laboratory and Dr. Ernelea P.
Cao’s laboratory.
•
Dr. Maria Corazon A. De Ungria was the Thesis Reader of Mr. Michael Herrera on his
Master of Science thesis under the Archaeological Studies Program.
Linkages
Local
-
Archaeological Studies Program (ASP)
Asia Against Child Trafficking (Asia-ACT) (NGO)
Armed Forces of the Philippines (AFP)
Asia Foundation
Biotechnology Coalition of the Philippines (BCP)
Child Protection-Network (CPU-Net)
Commission on Human Rights (CHR)
Department of Science and Technology ARMM
Department of Social Welfare and Development (DSWD)
EnGender Rights
House of Representatives – Committee on Science and Technology
International Justice Mission (IJM)
National Academy of Science and Technology (NAST)
National Institutes of Health (NIH)
National Meat Inspection Service (NMIS)
Network for Integrative Multidisciplinary Bioinformatics Utilization
Strategies Inc. (NIMBUS)
Outstanding Young Scientists Inc (OYSi Inc)
Philippine Dental Association (PDA)
Philippine Council for Advanced Science and Technology Research
Development (PCASTRD)
Philippine Carabao Center (PCC)
Philippine Dental Association (PDA)
Philippine National Police – Women and Children Protection Center
(PNPWCPC)
Philippine Science High School System (PSHS)
The Outstandng Women in the Nation Service (TOWNS)
International
-
Academy of Science for the Developing World (TWAS)
American Association of Blood Banks (AABB)
Asian Forensic Science Network (AFSN)
Asia- Pacific Association of Science and Technology
DNA-Prokids
Max Planck Institute for Evolutionary Anthropology (Germany)
University of Granada (Spain)
University of North Texas Health Science Center (USA)
Trainings conducted
1)
Title of Training
:
PROKIDS DNA Project: Forum on Using the DNA to Fight Child
Trafficking.
Funding Source(s)
:
Date
Trainor(s)
:
;
Philippine Council for Advanced Science and Technology
Research and Development (PCASTRD)
January 19, 2010
Dr. Jose Lorente
Dr. Arthur Eisenberg
Dr. Maria Corazon A. De Ungria
47
2)
Fees
:
No. of participants
:
Profile of participants :
none
~75-80
PNP Women’s and Children’s Desk Officers
Social Workers from DSWD and different NGO’S Lawyer
Title of Training
:
Symposium on Malaysian Human Genome Projects.
Funding Source(s)
Date
Trainor(s)
:
:
:
none
June 25, 2010
Dr. Zilfalil Alwi
Dr. Liza Sharmini Ahmad Tajudin
Dr. Endom Ismail
Dr. Surini Yusoff
Ms. Nur Shafawati Ab Rajab
Ms. Wan Nur Hatin Wan Isa
Ms. Sathiya Maran
none
~30
doctors and students
Fees
:
No. of participants
:
Profile of participants :
3)
Title of Training
:
Training on the National Referral Systems (NRS) for the
Recovery and Reintegration of Trafficked Persons and
National Recovery and Reintegration Database (NRRD).
Funding Source(s)
Date
Trainor(s)
:
:
:
Department of Social Welfare and Development
November 25, 2010
Dr. Maria Corazon A. De Ungria
Ms. Gayvelline C. Calacal
Ms. Minerva S. Sagum
none
25
Social Workers from DSWD
Fees
:
No. of participants
:
Profile of participants :
4)
Title of Training
:
Forensic DNA Technology During the Mass Disaster Response
for Philippine Coast Guard with Emphasis on the Recovery
and Identification of Human Remains.
Funding Source(s)
:
Date
Trainor(s)
:
:
Department of Science and Technology, Autonomous
Region in Muslim Mindanao and PCIEERD-DOST
November 9-12, 2010
Dr. Raquel Del Rosario Fortun
Dr. Maria Corazon A. De Ungria
Dr. Francisco Datar
Atty. Jose M. Jose
Ms. Gayvelline C. Calacal
none
~120
Coast Guards
Fees
:
No. of participants
:
Profile of participants :
Recent developments and other related activities of DAL for 2010
•
Laboratory citation/events/awards: Ms. Gayvelline C. Calacal was recognized
one of the Outstanding Young Scientist (field of Molecular Biology) by the National
Academy of Science and Technology (NAST) in its 32nd Annual Scientific Meeting.
•
Ms. Jazelyn M. Salvador finished her MS in Microbiology in May 2010 under the
Institute of Biology, College of Science, University of the Philippines Diliman.
48
•
Constructions/repairs:
Renovation of each section laboratories as part of the
“Development of the National Capability to conduct forensic DNA testing” grant
given by the House of Representatives from the 2007 National Budget, was finished
in the 2nd half 2010.
Visitors/Guests
Name of School/Agency
No. of visitors
Date of visit
-
University of Granada Spain
Dr. Jose A. Lorente
January 18-20, 2010
Name of School/Agency
-
No. of visitors
Date of visit
-
University of North Texas Center for Human
Identification
Dr. Arthur J. Eisenberg
January 18-20, 2010
Name of School/Agency
No. of visitors
Date of visit
-
The Rockefeller University
Dr. Torsten Wiesel (Emeritus Faculty)
January 27, 2010
Name of School/Agency
No. of visitors
Date of visit
-
UNICEF
Atty. Albert Muyot
January 29, 2010
Name of School/Agency
No. of visitors
-
Date of visit
-
UP- NIH, UPD (Archaeological Studies Program), NCIP
Dr. Carmencita Padilla
Dr. Eva Cutiongco-Dela Paz
Dr. Armand Mijares
Dr. Abe Padilla
Dr. Philip Piper
January 29, 2010
Name of School/Agency
No. of visitors
Date of visit
-
UP NIMBB
3 students
March 5, 2010
Name of School/Agency
-
No. of visitors
Date of visit
Name of School/Agency
No. of visitors
Date of visit
Cagayan De Oro College – Phinma Education
Network
- 8 teachers
- March 5, 2010
- Aklan State University
- 12 pax (teachers and students)
- May 20, 2010
Name of School/Agency
No. of visitors
Date of visit
-
UPD OVCRD
Ms. Gracious Romero
May 21, 2010
Name of School/Agency
No. of visitors
Date of visit
-
Nueva Vizcaya State University
55 pax (teachers and students)
August 26, 2010
Name of School/Agency
No. of visitors
-
Date of visit
-
Philippine Carabao Center
Dr. Jesus Rommel V. Herrera
Ms. Amie S. Villanueva
Ms. Leslie Anne M. Del Barrio
September 16, 2010
49
Name of School/Agency
No. of visitors
Date of visit
- Philippine Science High School Clark Field,
Pampanga
- 58 pax (students and teachers)
- November 18, 2010
DAL’s Photo Gallery
Fig.10. Courtesy call made by Dr. Jose Lorente (University of Granada) and Dr. Arthur Eisenberg
(University of North Texas Center for Human Identification) to then UP President Emerlinda Roman and
Chancellor Sergio Cao, Jan 18, 2010 University of the Philippines Diliman.
Fig.11. Asian Forensic Sciences
Network (AFSN) 2nd Annual
Meeting and Symposium. 1-3
June 2010. Empire Hotel and
Country Club, Jerudong, Brunei
Darussalam.
Fig.12. DNA team with world renowned
Forensic expert Dr. Henry Lee (from left to
right, Ms. Maria Catherine B. Otero, Ms.
Gayvelline C. Calacal, Dr. Henry Lee, Ms.
Minerva S. Sagum and Dr. Maria Corazon A.
De Ungria) during the Welcome Dinner for
the 2nd Annual Meeting and Symposium of
the Asian Forensic Science Network held in
Brunei Darussalam.
Fig.13. Ms. Gayvelline C. Calacal 2010
Outstanding Young Scientist (Molecular
Biology) awarded by the National
Academy of Science and Technology
of the Philippines (NAST), July 2010.
Manila Hotel Philippines.
Fig.14. Workshop on Forensic DNA technology during the Mass Disaster
Response for Philippine Coastguard with Emphasis on the Recovery
and Identification of Human Remains Seminar organized by the
Department of Science and Technology, Autonomous Region in
Muslim Mindanao and PCIEERD-DOST, Nov 9-12, 2010, Zamboanga
City. 2nd Row seated, 3rd from right is Atty. Jose M. Jose, Ms. Gayvelline
C. Calacal, Dr. Maria Corazon A. De Ungria and Dr. Raquel Del
Rosario-Fortun. 3rd from left is Dr. Francisco Datar.
Fig.15. Training on the National Referral Systems (NRS) for the Recovery and Reintegration of
Trafficked Persons and National Recovery and Reintegration Database (NRRD), Fontana Leisure
Parks, Pampanga, Nov 25, 2010, Pampanga.
Fig.16. The 2010 Search for Outstanding Thesis and Dissertation in Advanced Science and
Technology (Biology and Allied Fields Division) organized by Department of Science and
Technology – Philippine Council for Advanced Science and Technology (DOST-PCASTRD),
Intercontinental Hotel, Dec 10, 2010, Makati City.
Lectures and Meetings
Fig.17. Dr. Maria Corazon A. De Ungria at the
Philippine Carabao Center in Nueva Ecija,
March 26, 2010.
Fig.18. Doctors from the Ministry of Women
and Children Affairs of Bangladesh for a
Study tour, July 9, 2010.
Fig.19. Lecture during the Philippine Dental
Association Quezon Chapter 4th Scientific
Symposium, September 26, 2010.
Fig.20. Dr. Maria Corazon A. De Ungria
during the “TOWNS honors Awardees”,
October 15, 2010.
Laboratory tours of different schools
Fig.21. Nueva Vizcaya State University
Fig.22. Philippine Science High School Clark Field
Pampanga
Fig.23. Philippine Carabao Center
Fig.24. DNA Analysis Laboratory reach-out activity to the Reception and Study Center for Children of
the Department of Social Welfare and Development on December 6, 2010 at Quezon City.
Manpower Development
Activities of DAL Staff in 2010
1) MA. CORAZON A. DE UNGRIA (University Researcher III)
Position held
•
DNA Analysis Laboratory Head
Awards/Accreditation received
•
•
International Publication Awards given by the President of the University
of the Philippines.
Womanity Awards for Science (Finalist) by the Female Network.
Research Grants
•
•
•
•
•
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain.
Understanding the Genetic Diversity and Identity of Filipino Populations unded
by the Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
Forensic DNA Analysis of Vaginal Swab Samples from Child Victims of Sexual
Assault using Y-STR Multiplex Typing Procedure funded by the Office of Vice
Chancellor for Research and Development, University of the Philippines,
Diliman.
Validation of Extraction Methods for Human DNA from Used Cigarettes funded
by the Office of Vice Chancellor for Research and Development, University of
the Philippines, Diliman.
Validation of Procedures in DNA Extraction from Formalin-Fixed ParaffinEmbedded Tissues (FFPET) Samples funded by the Office of Vice Chancellor for
Research and Development, University of the Philippines, Diliman.
Evaluation of Real-time PCR Assays for Quantitation of Nuclear and
Mitochondrial DNA: Application to Forensic DNA Analysis funded by the Office
of Vice Chancellor for Research and Development, University of the Philippines,
Diliman.
Validation of Procedures for DNA Analysis of Meat Products funded by the
Natural Sciences Research Institute, University of the Philippines, Diliman.
Publications
•
•
•
•
Maiquilla SMB, Salvador JM, Calacal GC, Sagum MS, Dalet MRM, Delfin FC,
Tabbada KA, Franco SARL, Perdigon HB, Madrid BJ, Tan MP and De Ungria
MCA. Y-STR DNA Aalysis of 154 Female Child Sexual Assault Cases in the
Philippines. Int J Legal Medicine (in press).
Delfin F, Salvador JM, Calacal GC, Perdigon HB, Tabbada KA, Villamor LP, Halos
SC, Gunnarsdottir E, Myles S, Hughes DA, Xu S, Jin L, Lao O, Kayser M, Hurles ME,
Stoneking M and De Ungria MCA. The Y-Chromosome Landscape of the
Philippines: Extensive Heterogeneity and Varying Genetic Affinities of Negrito
and Non-Negrito Groups. Eur J Hum Genet (in press).
Maiquilla SMB, Salvador JM, Calacal GC, Delfin FC, Tabbada KA, Perdigon HB,
Dalet MRM, Sagum MS and De Ungria MCA. Expansion of the Philippine
Autosomal Short Tandem Repeat Population DFabatase for DNA-Based
Paternity Testing. Philippine Journal of Science 139(2): eprint.
Tabbada KA, Trejaut J, Loo JH, Chen YM, Lin M, Lahr MM, Kivisild T and De
Ungria MCA.(2010). Philippine Mitochondrial DNA Diversity: A Populated
Viaduct Between Taiwan and Indonesia? Molecular Biology and Evolution
(1):21-31.
Book Chapter
•
De Ungria MCA and Jose JM. Forensic DNA Profiling and Databasing: The
Philippine Experience. (2010). In Genetic Suspects: Global Governance of
Forensic DNA Profiling and Databasing. Edited by Hindmarsh R. and Praisack P.
Cambridge University Press, UK.
Lectures
•
•
•
•
•
•
•
De Ungria MCA. (2010). Advances in Molecular Analysis for Forensic
Applications. 11th National Genetics Symposium of the Philippine Society for the
Advancement of Genetics Inc with the theme ‘Genetics Education: Innovations
in Teaching and Learning Genetics’, Cavite State University, Philippines.
November 18-20, 2010.
De Ungria MCA. (2010). The Biologist as a Researcher. 1st Science Congress
organized by the Philippine Science Highschool System and the MMLDC
Foundation with the theme ‘Building a Culture of Science’, MMLDC, Philippines.
November 17, 2010.
De Ungria MCA. (2010). Forensic DNA Para sa Proteksiyon ng Karapatang
Pantao. Workshop for Paralegals organized by EnGender Rights, City Hall of
Quezon City, QC. October 28, 2010.
De Ungria MCA. (2010). Monitoring Laboratory Quality through DNA Testing.
Philippine Council for Quality Assurance in Clinical Laboratories (PCQACL)
during the 7th Annual Convention on ‘Quality Management: Raising the Bar in
Laboratory Medicine’. Crowne Plaza Galleria Manila, Ortigas Avenue, Pasig
City. September 29, 2010.
De Ungria MCA. (2010). DNA Testing for Human Identification. Philippine Dental
Association Quezon Province Dental Chapter during the 4th Quarterly Seminar
on ‘Updates on Forensic Odontology’. Queen Margarette Hotel, Lucena City.
Quezon. September 26, 2010.
De Ungria MCA. (2010). Genomics and DNA Databanking. Third World Network
on ‘Understanding BANG’. Bahay Kalinaw, UP Campus Diliman QC. September
23, 2010.
De Ungria MCA. (2010). Bringing Our Soldiers Back Home through DNA
Technology.
Scientific Seminar and Workshop: Updates on Forensic
Odontology for the delegates of the Armed Forces of the Philippines (AFP)
Dental Service and the Philippine Dental Association. Officers’ Club, Clark,
Pampanga. September 21, 2010.
55
•
•
•
•
•
•
•
De Ungria MCA. (2010). Insights of a Female Forensic Scientist in the Philippines
during the For Women in Science Symposium and Academic Launch of FWIS
National Fellowships - Philippines. UP College of Science, Diliman. September 20,
2010.
De Ungria MCA. (2010). DNA Testing for Paternity Determinations. Philippine
Dental Association Batangas Province Dental Chapter during the 4th Quarterly
Seminar on ‘Updates on Forensic Odontology’. St Frances Cabrini Medical
Center, Sto Tomas, Batangas. August 31, 2010.
De Ungria MCA. (2010). Bringing Our Soldiers Back Home through DNA
Technology. Scientific Seminar and Workshop: Updates on Forensic Odontology
for the delegates of the Armed Forces of the Philippines (AFP) Dental Service
and the Philippine Dental Association. Camp Aguinaldo, Quezon City. August
27, 2010.
De Ungria MCA. (2010). DNA Testing as a Tool for Paternity Exclusions. 27th
Convention of the Philippine Blood Coordinating Council (PBCC) with the
theme ‘From Vein to Vein: Quality Steps in Every Way’, EDSA Shangri-La Hotel,
Philippines. August 20, 2010.
De Ungria MCA. (2010). DNA and the Rights of Women and Children. Workshop
on the Multi-Sectoral Programme on Violence Against Women organized by the
Women and Child Protection Unit of the East Avenue Medical Center, University
of the Philippines, Quezon City. July 8, 2010.
De Ungria MCA. (2010). Metes and Bounds of DNA Profiling. 20th Annual
National Convention of the Philippine Criminologists Association of the
Philippines with the theme of ‘Criminologists and Forensic Sciences: Catapulting
Conduits in the Deterrence and Solving Crimes’. WaterFront Hotel, Davao City.
April 30 - 1 May 2010.
De Ungria MCA. (2010). Forensic Science as a Career. Career Fair, organized
by the Philippine Science High School, PSHS Main campus, Quezon City.
January 9, 2010.
Technical Consultations
•
•
•
•
Resource Person Training Workshop for Philippine Mass Disaster for Philippine
Coast Guards with Emphasis on Recovery and Identification of Human Remains,
organized by DOST-ARMM, Marcian Business Hotel, Zamboanga City,
Philippines. November 9-12, 2010.
Professorial Lecturer 1 for the Science and Technology Series, UP College of
Science (2009 to present).
Judge for the Asia Young Leader Program given by the Asia Society (20102011).
Resource person for ANC News and Public Affairs for its new program ‘Future
Perfect’ hosted by Mr. Tony Velasquez. August 18, 2010.
Conference Proceedings
•
•
•
•
Ferrer DE, Calacal GC, Salvador JM, Otero MCB and De Ungria MCA.
Validation of Extraction Methods for Human DNA from Cigarette Butts. 37th
Annual Convention of the Philippine Society for Biochemistry and Molecular
Biology.. Century Park Hotel Manila Philippines. December 2-3, 2010.
Otero MCB, Calacal GC, Salvador JM, Ferrer DEC and De Ungria MCA. Direct
PCR Amplification of Blood and Buccal Samples for Forensic DNA Databasing.
37th Annual Convention of the Philippine Society for Biochemistry and
Molecular Biology. Century Park Hotel Manila Philippines. December 2-3, 2010.
Salvador JM, Calacal GC, Rivera WL and De Ungria MCA. (2010).
DNaseproducing Bacteria in Exhumed Bones: Implications to Human DNA
Typing. 10th Convention of the Indo-Pacific Association of Law, Medicine and
Science. Amity University, New Dehli, India. October 25-30, 2010.
De Ungria MCA and Jose JM. (2010). Revolutionizing Philippine Policies Using
DNA Technology. Annual Meeting of the Asian Forensic Science Network.
Brunei Darussalam. June 1-3, 2010.
56
•
•
•
Calacal GC and De Ungria MCA. (2010). Estimation and Characterization of
Filamentous Fungi from Exhumed Skeletal Remains of Fire Victims. Annual
Meeting of the Asian Forensic Science Network. Brunei Darussalam. June 1-3,
2010.
Salvador JM, Calacal GC, Sagum MS, Maiquilla SMB, Franco SAEL, Otero MCB,
Dalet MRM and De Ungria MCA. (2010). Utility of Mitochondrial DNA Analysis in
Establishing Maternal Relationships: Case Reports. Annual Meeting of the Asian
Forensic Science Network. Brunei Darussalam, June 1-3, 2010.
Otero MCB, Calacal GC, Maiquilla SMB, Franco SAEL, Salvador JM, Ferrer DEC
and De Ungria MCA. (2010). Internal Validation of the AmpFlSTR® MiniFilerTM
PCR Amplification Kit for Analyzing Casework Samples. Annual Meeting of the
Asian Forensic Science Network. Brunei Darussalam, June 1-3, 2010.
Seminars/Workshops/Trainings attended
•
•
•
•
•
•
•
•
•
•
•
•
•
•
2nd Annual Meeting and Symposium of the Asian Forensic Science Network
(AFSN). Empire Hotel and Country Club, Brunei Darussalam. June 1-3, 2010.
DNA Workshop on DNA Mixture Interpretation conducted by Dr. Bruce Budowle.
Empire Hotel and Country Club, Brunei Darussalam. June 1, 2010.
Seminar-Workshop on ‘Total Quality/Workplace Management (5S Program)’ by
Dr Jorge V Sibal organized by the Natural Sciences Research Institute (NSRI).
University of the Philippines, Diliman. September 17, 2010.
Seminar on ‘The Origin and Evolution of Rice- a Molecular Perspective’ by Dr
Michael Purruganan organized by the Natural Sciences Research Institute
(NSRI). University of the Philippines, Diliman. August 4, 2010.
Knowledge Sharing Activity of the Karapatan sa Malikhaing Paraan (KaSaMa)
with the theme ‘Human Rights in the Aquino Administration’. University of the
Philippines, Diliman. July 22, 2010.
Scientific Meeting during the National Science and Technology Week (NSTW)
with the theme ‘Filipinnovation’. Manila Hotel, Philippines. July 20, 2010.
Workshop of the End Child Prostitution and Trafficking Philippines (ECPAT) on
‘Orientation on Organ Trafficking’. Quezon City, Philippines. July 16, 2010.
32nd Annual Scientific Meeting of the National Academy of Science and
Technology (NAST). ‘MDGs and Beyond: Are We Making Progress’. Manila Hotel,
Manila, Philippines. July 14-15, 2010.
5th Annual Meeting and Scientific Convention of the Outstanding Young
Scientists, Inc. (OYSI). ‘Meeting the Challenges of Natural Resource
Management’. Manila Hotel, Manila, Philippines. July 13, 2010.
Discussion Group on ‘Multisectoral Programme on Violence against Women’
organized by Women’s Crisis Center as part of the Study Tour of Representatives
from Bangladesh. Hotel Rembrandt, Quezon City. July 8-9, 2010.
Seminar on ‘The Philippine Genome Center’ organized by Division III, National
Research Council of the Philippines (NRCP). University of the Philippines, Manila.
June 28, 2010.
Seminar on the ‘Malaysian Human Genome Variation Consortium’ organized
by the Natural Sciences Research Institute. University of the Philippines, Diliman.
June 25, 2010.
Asia-Pacific Policy Forum on Human Rights organized by the Australian Embassy
and the Commission on Human Rights of the Philippines. Crowne Plaza Galleria
Hotel. June 17, 2010.
National Summit on Family Courts. ‘Strengthening the Multi-Sectoral Framework
for the Protection of Family and Children’ organized by the Supreme Court.
Manila Hotel, Manila, Philippines. March 12, 2010.
Organizations
International
•
DNA Workgroup of the Asia Forensic Science Network – Member and Country
Representative (2009-present).
57
•
•
•
•
•
•
Regional East Asia DNA Profiling Group (REAFD) - Member and Country
Representative (2007-2009)
The Academy of Science for the Developing World (TWAS) – Affiliate Fellow
(2007-2012)
American Association of Blood Banks- Member (2005-present)
Philippine-American Academy of Science and Engineering (PAASE)- Member
(2005-present)
International Society for Forensic Genetics - Member (2003-2004)
Third World Organization of Women Scientists (TWOWS) – Member (1999present)
Local
•
•
•
•
Outstanding Young Scientists Inc. – Board Member (Secretary 2008-2010)
Network for Integrative Multidisciplinary Bioinformatics Strategies (NIMBUS) Board Member (2007-present)
Philippine Society for Biochemistry and Molecular Biology (PSBMB) - Member
(2002-present)
National Research Council of the Philippines (Division V) – Member (2003present)
2) GAYVELLINE C. CALACAL (University Researcher II)
Awards/Accreditation received
•
•
International Publication Awards given by the President of the University of the
Philippines.
2010 Outstanding Young Scientist (Molecular Biology) awarded by the National
Academy of Science and Technology of the Philippines (NAST).
Research Grants
•
•
•
•
•
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain
Understanding the Genetic Diversity and Identity of Filipino Populations funded
by the Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
Forensic DNA Analysis of Vaginal Swab Samples from Child Victims of Sexual
Assault using Y-STR Multiplex Typing Procedure funded by the Office of Vice
Chancellor for Research and Development, University of the Philippines,
Diliman.
Validation of Extraction Methods for Human DNA from Used Cigarettes funded
by the Office of Vice Chancellor for Research and Development, University of
the Philippines, Diliman.
Validation of Procedures in DNA Extraction from Formalin-Fixed ParaffinEmbedded Tissues (FFPET) Samples funded by the Office of Vice Chancellor for
Research and Development, University of the Philippines, Diliman.
Evaluation of Real-Time PCR Assays for Quantitation of Nuclear and
Mitochondrial DNA: Application to Forensic DNA Analysis funded by the Office
of Vice Chancellor for Research and Development, University of the Philippines,
Diliman.
Validation of Procedures for DNA Analysis of Meat Products funded by the
Natural Sciences Research Institute, University of the Philippines, Diliman.
Publications
•
Maiquilla SMB, Salvador JM, Calacal GC, Sagum MS, Dalet MRM, Delfin FC,
Tabbada KA, Franco, SAE, Perdigon HB, Madrid BJ, Tan MP, and De Ungria. YSTR DNA Analysis of 154 Female Child Sexual Assault Cases in the Philippines. Int
J Legal Med. eprint.
58
•
•
•
Delfin FC, Salvador JM, Calacal GC, Perdigon HB, Tabbada KA, Villamor LP,
Halos SC, Gunnarsdottir E, Myles S, Hughes DA, Xu S, Jin L, Lao O, Kayser M,
Hurles ME, Stoneking M and De Ungria MCA. The Y-chromosome Landscape in
the Philippines: Extensive Heterogeneity and Varying Genetic Affinities of
Negrito and Non-Negrito Groups. European Journal of Human Genetics. eprint.
Maiquilla SMB, Salvador JM, Calacal GC, Delfin FC, Tabbada KA, Perdigon HB,
Dalet MRM, Sagum MS and De Ungria. Expansion of the Philippine Autosomal
Short Tandem Repeat Population Database for DNAbased Paternity Testing.
Philippine Journal Of Science 139(2) (in press).
Xu S, Kangwanpong D, Seielstad M, Srikummool M, Kampuansai J, Jin L. The
HUGO Pan Asia SNP Consortium. (2010). Genetic Evidence Supports Linguistic
Affinity of Mlabri--A hunter-Gatherer Group in Thailand. BMC Genet 11:18.
Lectures
•
•
•
•
Resource Person on DNA Documentation for the DNA-PROKIDS project during
the Training on the National Referral Systems (NRS) for the Recovery and
Reintegration of Trafficked Persons and National Recovery and Reintegration
Database (NRRD) organized by the Department of Social Welfare and
Development. Fontana Leisure Parks, Pampanga, Philippines. November 25,
2010.
Invited to deliver a lecture on Forensic DNA technology during the Mass Disaster
Response for Philippine Coastguard with Emphasis on the Recovery and
Identification of Human Remains organized by the Department of Science and
Technology, Autonomous Region in Muslim Mindanao and PCIEERD-DOST.
Marcian Business Hotel, Zamboanga City, Philippines. November 9-12, 2010.
Facilitator during the Symposium on Malaysian Human Genome Projects
organized by UP-NSRI-DNA Analysis Laboratory. NSRI, University of the
Philippines, Diliman Quezon City Philippines. June 25, 2010.
PROKIDS DNA Project: Forum on Using the DNA to Fight Child Trafficking.
Malcolm Hall, University of the Philippines, Diliman Quezon City Philippines.
January 19, 2010.
Conference Proceedings
•
•
•
•
•
•
Ferrer DE, Calacal GC, Salvador JM, Otero MCB and De Ungria MCA.
Validation of Extraction Methods for Human DNA from Cigarette Butts. 37th
Annual Convention of the Philippine Society for Biochemistry and Molecular
Biology. Century Park Hotel Manila, Philippines. December 2-3, 2010.
Otero MCB, Calacal GC, Salvador JM, Ferrer DEC and De Ungria MCA. Direct
PCR Amplification of Blood and Buccal Samples for Forensic DNA Databasing.
37th Annual Convention of the Philippine Society for Biochemistry and
Molecular Biology. Century Park Hotel Manila Philippines. December 2-3, 2010.
Salvador JM, Calacal GC, Rivera WL and De Ungria MCA. Dnase-Producing
Bacteria in Exhumed Bones: Implications to Human DNA Typing. 10th IndoPacific Congress on Legal Medicine and Forensic Science. Amity University,
New Delhi, India. October 25-30, 2010.
Calacal GC and De Ungria MCA. (2010). Estimation and Characterization of
Filamentous Fungi from Exhumed Skeletal Refmains of Fire Victims. Asian
Forensic Sciences Network (AFSN) 2nd Annual Meeting and Symposium. Empire
Hotel and Country Club, Jerudong, Brunei Darussalam. June 1-3, 2010.
Otero MCB, Calacal GC, Maiquilla SMB, Salvador JM, Ferrer DEC and De Ungria
MCA. Internal Validation of the AMPFISTR® Minifiler™ PCR Amplification Kit for
Analyzing Casework Samples. Asian Forensic Sciences Network (AFSN) 2nd
Annual Meeting and Symposium. Empire Hotel and Country Club, Jerudong,
Brunei Darussalam. June 1-3, 2010.
Salvador JM, Calacal GC, Sagum MS, Maiquilla SMB, Franco SAEL, Otero MCB,
Dalet MRM and De Ungria MCA. Utility of Mitochondrial DNA Analysis in
Establishing Maternal Relationships: Case Reports. Asian Forensic Sciences
59
Network (AFSN) 2nd Annual Meeting and Symposium. Empire Hotel and
Country Club, Jerudong, Brunei Darussalam. June 1-3, 2010.
Seminars and Trainings
•
•
•
•
•
•
•
•
•
•
“Saliva DNA Analysis” during the conduct of Forensic Odontology: A Post
Graduate Course to the delegates of the AFP Dental Service and Philippine
Dental Association. Officer’s Club, Air Force City, Clarkfield, Pampanga,
Philippines. September 2010.
For Women in Science Symposium and Academic Launch of FWIS National
Fellowships-Philippines.
College of Science Auditorium, University of the
Philippines, Diliman, Quezon City, Philippines. September 2010.
“Total Quality/Workplace Management (5S Program)”. NSRI Conference Room,
University of the Philippines, Diliman, Quezon City, Philippines. September 2010.
“Human Identification using DNA Technology” during the Updates on Forensic
Odontology. St. Frances Cabrini Medical Center, Maharlika Highway Sto. Tomas
Batangas. August 2010.
“Saliva DNA Analysis” during the Scientific Seminar and Workshop: Updates on
Forensic Odontology to the delegates of the AFP Dental Service and Philippine
Dental Association. AFP Commissioned Officers Club, Camp General Emilio
Aguinaldo, Quezon City, Philippines. August 2010.
32nd NAST Annual Scientific Meeting. MDG’s and Beyond: Are We Making
Progress? Manila Hotel, Philippines. July 2010.
5th Annual Meeting and Scientific Convention: Meeting the Challenges of
Natural Resource Management. Centennial Hall, Manila Hotel, Philippines. July
2010.
Symposium on “Networking and Technology Transfer” by INWENT, Hemholtz
Center for Infection Research and Queen Saovabha Memorial Institute (QSMI).
Montien Riverside Hotel, Bangkok, Thailand. June 2010.
Asian Forensic Sciences Network (AFSN) 2nd Annual Meeting and Symposium.
Empire Hotel and Country Club, Jerudong, Brunei Darussalam. June 2010.
Forum on “DNA-PROKIDS: Using DNA to Fight Child Trafficking”. Malcolm Hall
Theater, University of the Philippines, Diliman, Quezon City. January 19, 2010.
Organizations
International
•
•
DNA Workgroup of the Asia Forensic Science Network Representative (2009-present)
Regional East Asia DNA Profiling Group (REAFD) – Member
Member
Local
•
•
•
•
Philippine Biosafety and Biosecurity Association, Inc (PhBBA)
Philippine Society for Biochemistry and Molecular Biology (PSBMB)
Philippine Society for Microbiology (PSM)
Philippine Association of Medical Technologists (PAMET)
3) JAZELYN M. SALVADOR (University Research Associate I)
Awards/Accreditation received
•
•
Finalist. The 2010 Search for Outstanding Thesis and Dissertation in Advanced
Science and Technology (Biology and Allied Fields Division). Organized by
Department of Science and Technology – Philippine Council for Advanced
Science and Technology (DOST-PCASTRD).
International Publication Awards given by the President of the University of the
Philippines.
60
Research Grants
•
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain
Forensic DNA Analysis of Vaginal Swab Samples from Child Victims of Sexual
Assault using Y-STR Multiplex Typing Procedure funded by the Office of Vice
Chancellor for Research and Development, University of the Philippines,
Diliman.
Evaluation of real-time PCR assays for quantitation of nuclear and
mitochondrial DNA: application to forensic DNA analysis funded by the Office
of Vice Chancellor for Research and Development, University of the Philippines,
Diliman.
Publications
•
•
•
•
Maiquilla SMB, Salvador JM, Calacal GC, Sagum MS, Dalet MRM, Delfin FC,
Tabbada KA, Franco, SAE, Perdigon HB, Madrid BJ, Tan MP, and De Ungria. YSTR DNA Analysis of 154 Female Child Sexual Assault Cases in the Philippines. Int
J Legal Med. eprint.
Delfin FC, Salvador JM, Calacal GC, Perdigon HB, Tabbada KA, Villamor LP,
Halos SC, Gunnarsdottir E, Myles S, Hughes DA, Xu S, Jin L, Lao O, Kayser M,
Hurles ME, Stoneking M and De Ungria MCA. The Y-chromosome Landscape in
the Philippines: Extensive Heterogeneity and Varying Genetic Affinities of
Negrito and Non-Negrito Groups. European Journal of Human Genetics. eprint.
Maiquilla SMB, Salvador JM, Calacal GC, Delfin FC, Tabbada KA, Perdigon HB,
Dalet MRM, Sagum MS and De Ungria. Expansion of the Philippine Autosomal
Short Tandem Repeat Population Database for DNAbased Paternity Testing.
Philippine Journal Of Science 139(2) (in press).
Xu S, Kangwanpong D, Seielstad M, Srikummool M, Kampuansai J, Jin L. The
HUGO Pan Asia SNP Consortium. (2010). Genetic Evidence Supports Linguistic
Affinity of Mlabri--A hunter-Gatherer Group in Thailand. BMC Genet 11:18.
Lectures
•
•
Salvador JM. (2010). Identification of DNase-Producing Bacteria in Exhumed
Bones: Implications to Human DNA Typing. Philippine Council for Industry,
Energy and Emerging Technology Research and Development, Manila
Peninsula Hotel, Makati City. December 6, 2010.
Salvador JM. (2010). DNase-Producing Bacteria in Exhumed Bones: Implications
to Human DNA typing. 10th Indo-Pacific Congress on Legal Medicine and
Forensic Science, Amity University, Noida (near New Delhi), India. October 2530, 2010.
Conference Proceedings
•
•
•
•
Ferrer DE, Calacal GC, Salvador JM, Otero MCB and De Ungria MCA.
Validation of Extraction Methods for Human DNA from Cigarette Butts. 37th
Annual Convention of the Philippine Society for Biochemistry and Molecular
Biology. Century Park Hotel, Manila, Philippines. December 2-3, 2010.
Otero MCB, Calacal GC, Salvador JM, Ferrer DEC and De Ungria MCA. Direct
PCR Amplification of Blood and Buccal Samples for Forensic DNA Databasing.
37th Annual Convention of the Philippine Society for Biochemistry and
Molecular Biology. Century Park Hotel, Manila, Philippines. December 2-3, 2010.
Salvador JM, Calacal GC, Rivera WL and De Ungria MCA. (2010).
DNaseproducing Bacteria in Exhumed Bones: Implications to Human DNA
Typing. 10th-Indo-Pacific Congress on Legal Medicine and Forensic Science.
Amity University, Noida Campus, India. October 25-30, 2010.
Otero MCB, Calacal GC, Salvador JM, Franco SAEL, Maiquilla SMB, Ferrer DEC
and De Ungria MCA. (2010). Internal Validation of the AmpFlSTR® MiniFilerTM
PCR Amplification Kit for Analyzing Casework Samples. 2nd Annual Meeting and
61
•
Symposium of the Asian Forensic Sciences Network (AFSN). Brunei Darussalam.
June 1-3, 2010.
Salvador JM, Calacal GC, Sagum MS, Maiquilla SMB, Franco SAEL, Otero MCB,
Dalet MRM and De Ungria MCA. (2010). Utility of Mitochondrial DNA Analysis in
Establishing Maternal Relationships: Case Reports. 2nd Annual Meeting and
Symposium of the Asian Forensic Sciences Network (AFSN). Brunei Darussalam.
June 1-3, 2010.
Seminars and Trainings
•
•
•
•
10th Indo-Pacific Congress on Legal Medicine and Forensic Science. Amity
University, Noida Campus, India. October 25-30, 2010.
Seminar-Workshop on “Total Quality/Workplace Management (5S Program)”.
NSRI Conference Room, University of the Philippines, Diliman, Quezon City.
September 17, 2010.
Seminar-Workshop on “Web Content Development and Management using
JOOMLA”. Oxford Suites Hotel, Makati City. June 25-26, 2010.
Forum on “DNA-PROKIDS: Using DNA to Fight Child Trafficking”. Malcolm Hall
Theater, University of the Philippines, Diliman, Quezon City. January 19, 2010.
Organizations
International
•
Regional East Asia Forensic DNA (REAFD) Group. Member (Founding Member)
since 2007.
Local
•
•
•
•
•
•
Secretary, Network for Integrative Multidisciplinary Bioinformatics Utilization
Strategies, Inc. (NIMBUS Inc.) Lifetime Member.
Philippine Network of Microbial Culture Collections, Inc. Member since 2009.
Carl Duisberg Association of the Philippines, Inc. Member since March 2009.
Philippine Society for Microbiology. Member since 2004.
Philippine Society for Biochemistry and Molecular Biology. Member since 2002.
Philippine Society for Microbiology (PSM) – Member Since 2004.
4) MIRIAM RUTH M. DALET (University Research Associate I)
Awards/Accreditation received
•
International Publication Award given by the President of the University of the
Philippines.
Research Grants
•
•
•
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain
Understanding the Genetic Diversity and Identity of Filipino Populations funded
by the Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
Forensic DNA Analysis of Vaginal Swab Samples from Child Victims of Sexual
Assault using Y-STR Multiplex Typing Procedure funded by the Office of Vice
Chancellor for Research and Development, University of the Philippines,
Diliman.
Validation of Extraction Methods for Human DNA from Used Cigarettes funded
by the Office of Vice Chancellor for Research and Development, University of
the Philippines, Diliman.
Validation of Procedures in DNA Extraction from Formalin-Fixed ParaffinEmbedded Tissues (FFPET) Samples funded by the Office of Vice Chancellor for
Research and Development, University of the Philippines, Diliman.
62
•
•
Evaluation of real-time PCR assays for quantitation of nuclear and
mitochondrial DNA: application to forensic DNA analysis funded by the Office
of Vice Chancellor for Research and Development, University of the Philippines,
Diliman.
Validation of Procedures for DNA analysis of meat products funded by the
Natural Sciences Research Institute, University of the Philippines, Diliman.
Publications
•
•
Maiquilla SMB, Salvador JM, Calacal GC, Sagum MS, Dalet MRM, Delfin FC,
Tabbada KA, Franco SARL, Perdigon HB, Madrid BJ, Tan MP and De Ungria
MCA. Y-STR DNA analysis of 154 female child sexual assault cases in the
Philippines. Int J Legal Medicine (in press).
Maiquilla SMB, Salvador JM, Calacal GC, Delfin FC, Tabbada KA, Perdigon HB,
Dalet MRM, Sagum MS and De Ungria MCA. Expansion of the Philippine
Autosomal Short Tandem Repeat Population DFabatase for DNA-based
Paternity Testing. Philippine Journal of Science 139(2): eprint.
Conference Proceedings
•
Salvador JM, Calacal GC, Sagum MS, Maiquilla SMB, Franco SAEL, Otero MCB,
Dalet MRM and De Ungria MCA. (2010). Utility of Mitochondrial DNA Analysis in
Establishing Maternal Relationships: Case Reports. Annual Meeting of the Asian
Forensic Science Network. Brunei Darussalam. 1-3 June 2010.
Seminars and Trainings
•
•
“Total Quality/Workplace Management (5S Program)”. NSRI Conference Room,
University of the Philippines, Diliman, Quezon City, Philippines. September 2010.
Forum on “DNA-PROKIDS: Using DNA to Fight Child Trafficking”. Malcolm Hall
Theater, University of the Philippines, Diliman, Quezon City. January 19, 2010.
Organizations
International
•
Regional East Asia Forensic DNA (REAFD) Group. Member (Founding Member)
since 2007
Local
•
Philippine Society for Microbiology (PSM) – Member
5) MINERVA S. SAGUM (University Research Associate I)
Awards/Accreditation received
•
International Publication Award given by the President of the University of the
Philippines.
Research Grants
•
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain
Understanding the Genetic Diversity and Identity of Filipino Populations funded
by the Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
Forensic DNA Analysis of Vaginal Swab Samples from Child Victims of Sexual
Assault using Y-STR Multiplex Typing Procedure funded by the Office of Vice
Chancellor for Research and Development, University of the Philippines,
Diliman.
63
•
•
•
•
Validation of Extraction Methods for Human DNA from Used Cigarettes funded
by the Office of Vice Chancellor for Research and Development, University of
the Philippines, Diliman.
Validation of Procedures in DNA Extraction from Formalin-Fixed ParaffinEmbedded Tissues (FFPET) Samples funded by the Office of Vice Chancellor for
Research and Development, University of the Philippines, Diliman.
Evaluation of real-time PCR assays for quantitation of nuclear and
mitochondrial DNA: Aapplication to Forensic DNA Analysis funded by the Office
of Vice Chancellor for Research and Development, University of the Philippines,
Diliman.
Validation of Procedures for DNA Analysis of Meat Products funded by the
Natural Sciences Research Institute, University of the Philippines, Diliman.
Publications
•
•
Maiquilla SMB, Salvador JM, Calacal GC, Sagum MS, Dalet MRM, Delfin FC,
Tabbada KA, Franco SARL, Perdigon HB, Madrid BJ, Tan MP and De Ungria
MCA. Y-STR DNA Analysis of 154 Female Child Sexual Assault Cases in the
Philippines. Int J Legal Medicine (in press).
Maiquilla SMB, Salvador JM, Calacal GC, Delfin FC, Tabbada KA, Perdigon HB,
Dalet MRM, Sagum MS and De Ungria MCA. Expansion of the Philippine
Autosomal Short Tandem Repeat Population DFabatase for DNA-based
Paternity Testing. Philippine Journal of Science 139(2): eprint.
Conference Proceedings
•
Salvador JM, Calacal GC, Sagum MS, Maiquilla SMB, Franco SAEL, Otero MCB,
Dalet MRM and De Ungria MCA. Utility of Mitochondrial DNA Analysis in
Establishing Maternal Relationships: Case Reports. Asian Forensic Sciences
Network (AFSN) 2nd Annual Meeting and Symposium. Empire Hotel and Country
Club, Jerudong, Brunei Darussalam. June 1-3, 2010.
Seminars and Trainings
•
•
•
•
•
•
•
“Saliva DNA Analysis” during the conduct of Forensic Odontology: A Post
Graduate Course to the delegates of the AFP Dental Service and Philippine
Dental Association. Officer’s Club, Air Force City, Clarkfield, Pampanga,
Philippines. September 2010.
“Total Quality/Workplace Management (5S Program)”. NSRI Conference Room,
University of the Philippines, Diliman, Quezon City, Philippines. September 2010.
“Human Identification using DNA Technology” during the Updates on Forensic
Odontology. St. Frances Cabrini Medical Center, Maharlika Highway Sto. Tomas
Batangas. August 2010.
“Saliva DNA Analysis” during the Scientific Seminar and Workshop: Updates on
Forensic Odontology to the delegates of the AFP Dental Service and Philippine
Dental Association. AFP Commissioned Officers Club, Camp General Emilio
Aguinaldo, Quezon City, Philippines. August 2010.
32nd NAST Annual Scientific Meeting. MDG’s and Beyond: Are We Making
Progress? Manila Hotel, Philippines. July 2010.
Asian Forensic Sciences Network (AFSN) 2nd Annual Meeting and Symposium.
Empire Hotel and Country Club, Jerudong, Brunei Darussalam. June 2010.
Forum on “DNA-PROKIDS: Using DNA to Fight Child Trafficking”. Malcolm Hall
Theater, University of the Philippines, Diliman, Quezon City. January 19, 2010.
Organizations
International
•
Regional East Asia Forensic DNA (REAFD) Group. Member (Founding Member)
since 2007.
64
Local
•
•
•
Philippine Society for Microbiology (PSM) – Member
Philippine Association for Teacher Education (PAFTE) – Member
Integrated Chemists of the Philippines (ICP) – Associate Member
6) SHEENA MARIE B. MAIQUILLA (University Research Associate I, January – July 2010)
Awards/Accreditation received
•
International Publication Award given by the President of the University of the
Philippines.
Research Grants
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain
Forensic DNA Analysis of Vaginal Swab Samples from Child Victims of Sexual
Assault using Y-STR Multiplex Typing Procedure funded by the Office of Vice
Chancellor for Research and Development, University of the Philippines,
Diliman.
Publications
•
•
Maiquilla SMB, Salvador JM, Calacal GC, Sagum MS, Dalet MRM, Delfin FC,
Tabbada KA, Franco SARL, Perdigon HB, Madrid BJ, Tan MP and De Ungria
MCA. Y-STR DNA Analysis of 154 Female Child Sexual Assault Cases in the
Philippines. Int J Legal Medicine (in press).
Maiquilla SMB, Salvador JM, Calacal GC, Delfin FC, Tabbada KA, Perdigon HB,
Dalet MRM, Sagum MS and De Ungria MCA. Expansion of the Philippine
Autosomal Short Tandem Repeat Population DFabatase for DNA-based
Paternity Testing. Philippine Journal of Science 139(2): eprint.
Conference Proceedings
•
•
Otero MCB, Calacal GC, Maiquilla SMB, Salvador JM, Ferrer DEC and De Ungria
MCA. Internal Validation of the AMPFISTR® Minifiler™ PCR Amplification Kit for
Analyzing Casework Samples. Asian Forensic Sciences Network (AFSN) 2nd
Annual Meeting and Symposium. Empire Hotel and Country Club, Jerudong,
Brunei Darussalam. 1-3 June 2010.
Salvador JM, Calacal GC, Sagum MS, Maiquilla SMB, Franco SAEL, Otero MCB,
Dalet MRM and De Ungria MCA. Utility of Mitochondrial DNA Analysis in
Establishing Maternal Relationships: Case Reports. Asian Forensic Sciences
Network (AFSN) 2nd Annual Meeting and Symposium. Empire Hotel and
Country Club, Jerudong, Brunei Darussalam. 1-3 June 2010.
Seminars and Trainings
•
•
32nd NAST Annual Scientific Meeting. MDG’s and Beyond: Are We Making
Progress? Manila Hotel, Philippines. July 2010.
Forum on “DNA-PROKIDS: Using DNA to Fight Child Trafficking”. Malcolm Hall
Theater, University of the Philippines, Diliman, Quezon City. January 19, 2010.
Organizations
Local
•
Network
for
Integrative Multi-disciplinary Bioinformatics
Strategies/NIMBUS (Member, November 2009 - present)
Utilization
65
•
•
•
•
Philippine Biosafety and Biosecurity Association Inc. (Member, March 2009 present)
Philippine Society of Parasitology (Member, 2008 - present)
Philippine Society for Microbiology, Inc. (Member, 2007 - present)
Phi Sigma Biological Honor Society (Member, 2006 - present)
7) MARIA CATHERINE B. OTERO (University Research Associate I)
Research Grants
•
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain
Validation of Procedures in DNA Extraction from Formalin-Fixed ParaffinEmbedded Tissues (FFPET) Samples funded by the Office of Vice Chancellor for
Research and Development, University of the Philippines, Diliman.
Validation of Procedures for DNA analysis of meat products funded by the
Natural Sciences Research Institute, University of the Philippines, Diliman.
Conference Proceedings
•
•
•
•
Ferrer DE, Calacal GC, Salvador JM, Otero MCB and De Ungria MCA.
Validation of Extraction Methods for Human DNA from Cigarette Butts. 37th
Annual Convention of the Philippine Society for Biochemistry and Molecular
Biology. Century Park Hotel Manila Philippines. December 2-3, 2010.
Otero MCB, Calacal GC, Salvador JM, Ferrer DEC and De Ungria MCA. Direct
PCR Amplification of Blood and Buccal Samples for Forensic DNA Databasing.
37th Annual Convention of the Philippine Society for Biochemistry and Molecular
Biology. Century Park Hotel Manila Philippines. December 2-3, 2010.
Otero MCB, Calacal GC, Maiquilla SMB, Salvador JM, Ferrer DEC and De Ungria
MCA. Internal Validation of the AMPFISTR® Minifiler™ PCR Amplification Kit for
Analyzing Casework Samples. Asian Forensic Sciences Network (AFSN) 2nd
Annual Meeting and Symposium. Empire Hotel and Country Club, Jerudong,
Brunei Darussalam. June 1-3, 2010.
Salvador JM, Calacal GC, Sagum MS, Maiquilla SMB, Franco SAEL, Otero MCB,
Dalet MRM and De Ungria MCA. Utility of Mitochondrial DNA Analysis in
Establishing Maternal Relationships: Case Reports. Asian Forensic Sciences
Network (AFSN) 2nd Annual Meeting and Symposium. Empire Hotel and Country
Club, Jerudong, Brunei Darussalam. June 1-3, 2010.
Seminars and Trainings
•
•
•
•
37th Annual Philippine Society for Biochemistry and Molecular Biology (PSBMB)
Convention. Century Park Hotel, Manila. December 2-3, 2010.
“Total Quality/Workplace Management (5S Program)”. NSRI Conference Room,
University of the Philippines, Diliman, Quezon City, Philippines. September 2010.
Asian Forensic Sciences Network (AFSN) 2nd Annual Meeting and Symposium.
Empire Hotel and Country Club, Jerudong, Brunei Darussalam. June 2010.
Forum on “DNA-PROKIDS: Using DNA to Fight Child Trafficking”. Malcolm Hall
Theater, University of the Philippines, Diliman, Quezon City. January 19, 2010.
Organizations
Local
•
•
Philippine Society of Animal Science - Member
Philippine Society for Biochemistry and Molecular Biology – Member
66
8) DOROTHY EMMA C. FERRER (Laboratory Research Associate, Oct. 2008 – Aug. 2010)
(University Research Associate 1, Aug. 2010 – Feb. 2011)
Research Grants
•
•
•
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain
Validation of Extraction Methods for Human DNA from Used Cigarettes funded
by the Office of Vice Chancellor for Research and Development, University of
the Philippines, Diliman.
Evaluation of Real-Time PCR Assays for Quantitation of Nuclear and
Mitochondrial DNA: Application to Forensic DNA Analysis funded by the Office
of Vice Chancellor for Research and Development, University of the Philippines,
Diliman.
Conference Proceedings
•
•
•
Ferrer DE, Calacal GC, Salvador JM, Otero MCB and De Ungria MCA.
Validation of Extraction Methods for Human DNA from Cigarette Butts. 37th
Annual Convention of the Philippine Society for Biochemistry and Molecular
Biology. Century Park Hotel Manila Philippines. December 2-3, 2010.
Otero MCB, Calacal GC, Salvador JM, Ferrer DEC and De Ungria MCA. Direct
PCR Amplification of Blood and Buccal Samples for Forensic DNA Databasing.
37th Annual Convention of the Philippine Society for Biochemistry and Molecular
Biology. Century Park Hotel Manila Philippines. December 2-3, 2010.
Otero MCB, Calacal GC, Maiquilla SMB, Salvador JM, Ferrer DEC and De Ungria
MCA. Internal Validation of the AMPFISTR® Minifiler™ PCR Amplification Kit for
Analyzing Casework Samples. Asian Forensic Sciences Network (AFSN) 2nd
Annual Meeting and Symposium. Empire Hotel and Country Club, Jerudong,
Brunei Darussalam. June 1-3, 2010.
Seminars and Trainings
•
•
•
37th Annual Philippine Society for Biochemistry and Molecular Biology (PSBMB)
Convention. Century Park Hotel, Manila. December 2-3, 2010.
“Total Quality/Workplace Management (5S Program)”. NSRI Conference Room,
University of the Philippines, Diliman, Quezon City, Philippines. September 2010.
Forum on “DNA-PROKIDS: Using DNA to Fight Child Trafficking”. Malcolm Hall
Theater, University of the Philippines, Diliman, Quezon City. January 19, 2010.
Organizations
International
•
Chesapeake Bay Division – International Association for Identification Associate
Member
Local
•
Integrated Chemists of the Philippines (ICP) – Member
9) FREDERICK C. DELFIN (University Research Associate I, on study leave)
Awards/Accreditation received
•
•
International Publication Award given by the President of the University of the
Philippines.
PhD Program on Understanding the Genetic Diversity and Identity of Filipino
Populations funded by the Max Planck Institute for Evolutionary Anthropology,
Leipzig, Germany.
67
Research Grants
•
Understanding the Genetic Diversity and Identity of Filipino Populations funded
by the Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
Publications
•
•
•
•
Maiquilla SMB, Salvador JM, Calacal GC, Sagum MS, Dalet MRM, Delfin FC,
Tabbada KA, Franco, SAE, Perdigon HB, Madrid BJ, Tan MP, and De Ungria. YSTR DNA Analysis of 154 Female Child Sexual Assault Cases in the Philippines. Int
J Legal Med. eprint.
Delfin FC, Salvador JM, Calacal GC, Perdigon HB, Tabbada KA, Villamor LP,
Halos SC, Gunnarsdottir E, Myles S, Hughes DA, Xu S, Jin L, Lao O, Kayser M,
Hurles ME, Stoneking M and De Ungria MCA. The Y-chromosome Landscape in
the Philippines: Extensive Heterogeneity and Varying Genetic Affinities of
Negrito and non-Negrito Groups. European Journal of Human Genetics. eprint.
Maiquilla SMB, Salvador JM, Calacal GC, Delfin FC, Tabbada KA, Perdigon HB,
Dalet MRM, Sagum MS and De Ungria. Expansion of the Philippine Autosomal
Short Tandem Repeat Population Database for DNA-based Paternity Testing.
Philippine Journal of Science 139(2) (in press).
Xu S, Kangwanpong D, Seielstad M, Srikummool M, Kampuansai J, Jin L. The
HUGO Pan Asia SNP Consortium. (2010). Genetic Evidence Supports Linguistic
Affinity of Mlabri--A Hunter-Gatherer Group in Thailand. BMC Genet 11:18.
10) EUGENE JOHN F. BALMORES, JR. (Laboratory Research Associate, Nov. 2010 - present)
Research Grants
•
•
•
•
Amplification of HVI and HVII regions of the mtDNA using primers obtained from
the Laboratory of Genetic Identification, University of Granada.
DNA Testing of Child Trafficking Cases funded by the DNA-Prokids Initiative,
Granada, Spain.
Diverse Drug Leads from Philippine Marine Mollusc Bacterial Symbionts with the
Marine Natural Products Laboratory, Marine Science Institute, University of the
Philippines.
Conus and Turrid Snails: Biochemistry, Bioassay, Drug Discovery and
Development with the Marine Natural Products Laboratory, Marine Science
Institute, University of the Philippines.
Seminars and Trainings
•
Weekly Scientific Journal Seminar with the Marine Natural Products Laboratory,
Marine Science Institute, University of the Philippines.
MICROBIOLOGICAL RESEARCH AND SERVICES LABORATORY (MRSL)
With its vision to be a vital microbial resource center, the Microbiological Research
and Services Laboratory (MRSL) has continually provided quality microbial cultures and
services to the different sectors of the society. The laboratory has supported research work by
students, educators and researchers. MRSL has also served as partners of various industrial
firms in the implementation of their Research and Development (R & D) and Quality Assurance
(QC) programs.
Furthermore, MRSL has supported the human resource development programs of
government agencies, educational institutions and industrial firms through the training course
on “Bacteriological Examination of Water” and a workshop on the
Characterization using the Biolog Gen III Microbial Identification System”.
“Phenotypic
Research endeavors by the MRSL staff were focused on microorganisms from marine
sponges and their antimicrobial properties, microorganisms from lake and environmental
samples and lactic acid bacteria from fermented foods.
In line with its plans for ISO 17025 accreditation, the MRSL Researchers participated in
the 2010 Philippine MSTQ, Inc. National Microbiological Proficiency Testing Program for Food
Matrix, Non-pathogens category, in September 2010. The MRSL researchers demonstrated
their competence in the enumeration of eight (8) microbial parameters, under the nonpathogen category, namely, Aerobic Plate Count, Bacillus cereus Count, Coliform Count,
sFecal Coliform Count, E. coli Count, Lactic Acid Bacteria Count, Mold Count and Yeast
Count.
Through the equipment grant from the National Science Complex, MRSL was able to
acquire two equipment, namely, a stomacher and the Biolog Gen III Microbial Identification
System. With the new stomacher or homogenizer, food homogenate preparation for
microbial analyses is facilitated, ensuring the generation of accurate and reliable results. The
Biolog Gen III system is a semi-automated system that identifies environmental and clinical
microorganisms using metabolic fingerprint generated throught its carbon source utilization
technology. Using the Biolog Gen III machine which has a database for aerobic and
anaerobic bacteria, filamentous fungi and yeasts, phenotypic characterization of of the
microbial cultures in the UPCC as well as the new isolates from the projects could be
undertaken. Identification services using the system will also be offered in the future. With its
installation in MRSL, a training-workshop on Biolog Omnilog Gen III System for Microbial
Identification was conducted by Infocus Biotech technical personnel, Ms. Cherlin Ooi, on
November 8 to 9, 2010. It was attended by the following researchers: a) from MRSL - Ms. Vina
B. Argayosa, Ms. Sonia Sp. Bulaong, Ms. Celia A. Cereno, Mr. Angelo D. Dela Tonga, Ms. Maria
Arlin A. Laguitan and Dr. Maria Auxilia T. Siringan, b) from the University of the Philippines, Los
Baños (UPLB) – Dr. Marian P. De Leon and c) from University of Santo Tomas (UST) – Dr. Gina R.
Dedeles. MRSL researchers were also trained in the calibration of pipetors by Ms. Anita Chng,
Thermo Scientific Commercial Product Manager. The pipet calibration workshop was
sponsored by Molave Trading.
Researches
Renewed in 2010
DOST-PCAMRD Project 2 – Marine Microorganisms: Characterization and
Culture Optimization under the Program, Philippine Pharmaseas Drug Discovery
Program - Marine Drug Discovery from Marine Microorganisms for Anti-Infective Agents
and from Turrid Snails for Anti-Pain Agents” (PHIL-PHARMSEAS).
Project leader:
Researchers:
Maria Auxilia T. Siringan
Mary Ann Cielo V. Relucio
Angelo D. Dela Tonga
Maria Arlin A. Laguitan
On-going In-house research
MRSL provided research funds and facilities in the conduct of the following studies:
• Isolation and Identification of Chromobacterium violaceum from Laguna de Bay
(Pangil, Laguna)
Study leader: Vina B. Argayosa
• Isolation and identification of lactic acid bacteria from fermented foods.
Study leader: Sonia Sp. Bulaong
69
Extension Services/Clients
Culture Collection
The University of the Philippines Culture Collection (UPCC) of the MRSL maintains
a total of 432 microbial cultures, consisting of 135 bacterial, 219 fungal and 78 yeast
cultures. Of these cultures, 402 are cultures listed in the UPCC Catalogue of Cultures
(2000) and in 2006 edition of the Directory of Culture Collections of the Philippines. A
total of 30 cultures are categorized as unlisted or with restricted distribution. These are
for the exclusive use of the depositors or importers. In addition, four ATCC reference
cultures were acquired by the laboratory for in-house assays. These cultures are Bacillus
subtilis ATCC 6633, Candida albicans ATCC 10231, Escherichia coli ATCC 25922 and
Staphylococcus aureus ATCC 6638. Table 2 presents the current UPCC microbial
holdings.
Table 2. The UPCC Microbial Holdings.
Category
Bacteria
Fungi
Yeast
Total
Listeda
Unlistedb
Subtotals
122
13
135
207
12
219
73
5
78
402
30
432
MRSL has distributed viable and pure microbial cultures to individuals and
institutions involved in instruction, research, quality assurance program, and product
development. Of the 512 microbial cultures distributed in 2010, approximately 72%
(367) were bacteria, 16% (83) were fungi and 12% (62) were yeasts (Fig.25). The major
users of microbial cultures are from educational institutions (72%), particularly students,
educators, and researchers from various schools and universities (Fig.26). The industrial
sector (19.7%) was the second major user of microbial cultures, which are primarily
used as reference cultures and test organisms in the R&D and QA programs of
manufacturing firms and testing laboratories.
12%
16%
72%
1
Bacteria
2
Fungi
3
Yeast
Fig.25. Distribution of Microbial Cultures in 2010 Based on Microbial Groups
1.4%
8.2%
19.7%
1 Type I
14.8%
2 Type II
0.8%
3 Type III
4 Type IV
55%
5 Type V
6 Type VI
Fig.26. Distribution of Microbial Cultures in 2010 Based on Client Types
Category
I
II
III
IV
V
VI
Client Type
College of Science (CS), UP Diliman students
NSRI-funded research projects
Non-NSRI funded researches and non-CS students
Non-UP students
Government agencies and Small to Medium Scale Enterprises (SMEs)
Companies
Microbiological Services
In 2010, MRSL performed a total of 6,377 microbiological services, consisting of
4,964 (77.85%) microbial analyses for different types of water (Table 3) and 1,413
(22.2%) various analytical services for beverage, food, food ingredients, personal care
products (cosmetics, toiletries and their raw materials), biocidal agents, plant-derived
compounds, tools and new materials (Table 4).
Table 3. Microbial services performed for various types of water in 2010.
Client
Potability
HPC
Detection of
P. aeruginosa
Detection of
fecal
Streptococcus
Fecal
Streptococcus
Count
Coliform
Level
Determination
Subtotal
(by Client
Type)
I
II
III
IV
0
3
0
644
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
3
0
644
V
VI
Subtotal
Total
291
2,854
3,792
320
417
737
0
3
3
0
6
6
47
0
47
363
16
379
1,021
3,296
4,964
4,964
More than 76% (3,792 out of 4,964) of the water samples was tested for potability testing
(Fig.26). Heterotrophic Plate Count (HPC) comprised about 15 % of the tests performed for
water samples. The major client for microbiological analyses of water (Fig.27) is the industrial
sector (66.4%) followed by the group consisting of government agencies and small to mediumscale enterprises (20.6%).
MRSL has also supported the water quality monitoring program of the Laguna Lake
Development Authority (LLDA) in 2010. Samples from Laguna Lake and its tributaries were
submitted for fecal streptococcus count and coliform level determination. Through the
technical services provided by the MRSL, LLDA was able to continue its monitoring activities
while renovation of the laboratory and its facilities is in progress.
7.6%
15%
76.4%
1
Potability
2
HPC
3
Pseudomonas detection
4
Fecal streptococci detection
5
Fecal streptococci count
6
Coliform level determination
Fig.26. Microbiological Analyses Performed for Various Types of Water in 2010.
13%
1 Type I
20.6%
2 Type II
3 Type III
66.4%
4 Type IV
5 Type V
6 Type VI
Fig.27. Client Types that Have Availed of Microbiological Analyses of Various Types of Wate in 2010.
Category
I
II
III
IV
V
VI
Client Type
College of Science (CS), UP Diliman students
13%
NSRI-funded research
projects
Non-NSRI funded researches and non-CS students
Non-UP students
20.6%
Government agencies and Small to Medium Scale Enterprises (SMEs)
Companies
A total of 1,413 microbiological analyses of non-water commodities and products were
performed in 2010 (Table 4). The top microbiological analyses conducted in 2010 are the
following: a) Aerobic Plate Count or APC (597 out of 1,413 or 42.3%), b) qualitative screening
or antimicrobial assay of different bioactive compounds or extracts (514/1,413 or 36.4%), and
c) detection of E.coli or coliform bacteria (95/1,413 or 6.7%).
Table 4.
List of microbiological services of non-water samples rendered for the period: January to
December 2010.
Type of Microbiological Services
Total plate count/Aerobic plate count
Yeast and mold
Detection of E. coli/coliform
Detection of Staphylococcus aureus
Detection of P. aeruginosa
Detection of Salmonella
Detection of Candida albicans
Detection of Bacillus cereus
Detection of Bulkholderia cepacia growth
Azospirillum count
Bacillus subtilis count
Swab count
Qualitative screening of compounds/Antimicrobial assay
Percent kill
Minimum inhibitory concentration
Identification of molds
Identification of fungus
Identification of floater
Identification of growth
ASTM mold resistance test
Isolation and purification of aerobic bacteria
I
II
III
IV
V
VI
Subtotal
0
0
0
0
0
0
0
0
0
0
0
0
91
0
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
4
0
0
0
1
0
0
0
0
0
0
2
0
0
0
0
0
0
0
0
0
248
2
3
0
0
0
0
0
0
1
1
1
0
0
0
0
0
0
0
0
0
171
0
0
1
0
0
0
0
0
596
77
95
9
1
28
1
2
8
8
2
6
0
32
4
0
2
3
1
6
1
597
78
98
9
1
28
1
2
8
8
2
6
514
34
9
1
3
3
1
6
1
0
0
0
0
0
1
1
0
0
1
0
0
1
2
6
255
175
884
1,413
Characterization and identiification of aerobic
bacteria
Confirmation of identification of bacterial isolate
93
Subtotal
0
1,413
Total
The industrial sector remains to be the major client of the laboratory in relation to
microbiological analyses of non-water samples (Fig.28). Nearly 63% of the 1,413 analyses
performed were requested by manufacturing firms and distributors of various commodities
from food to environmental samples.
0.42 %
6.6 %
18 %
1 Type I
2 Type II
63 %
12.4 %
3 Type III
4 Type IV
5 Type V
6 Type VI
Fig.28. Client types that have availed of microbiological analyses of non-water samples in 2010.
Category
I
II
III
IV
V
VI
Client Type
College of Science (CS), UP Diliman students
NSRI-funded research projects
Non-NSRI funded researches and non-CS students
Non-UP students
Government agencies and Small to Medium Scale Enterprises (SMEs)
Companies
Support to Students
MRSL researchers have served the academic community through their involvement in
the completion of thesis requirements of UP and non-UP students. Dr. Maria Auxilia T. Siringan
and Ms. Vina B. Argayosa were tapped as members of thesis panels in various capacities
(Table 5).
The MRSL Researchers have also assisted the students from secondary schools and
universities in their investigatory studies and undergraduate research work through the
provision of technical services and advice (Table 6). In addition, students from the College of
Science (CS) and College of Engineering (COE) have availed of the use of the equipment and
facilities of the laboratory such as the analytical balance, autoclave and biological safety
cabinet. Thesis advisees of Prof. Marilen M. Parungao of UP Manila conducted their study
using the L-drying equipment of the MRSL, under the supervision of Dr. Siringan and Mr.
Reynaldo E. Javate, Jr.
In addition, Dr. Siringan served as trainor and supervisor of Ms. Noelle Nikita Santos, a 4th
year BS Biology student, Our Lady of Fatimah University, Fairview, Quezon City. Ms. Santos was
trained in microbiological techniques under a student apprenticeship program from June to
September 2010. The apprenticeship is part of the requirements for the completion of her
degree. Ms. Mary Adele N. Arjona and Mr. Javate assisted Dr. Siringan in the program.
Table 5. Involvement of MRSL Researchers in Thesis Work of College of Science (CS) Students in 2010.
Name of Student and
Degree
Richard F. Clemente
PhD Biology
Deo Florence L. Onda,
MS Marine Science
Donna Salve P. Cornes
MS Biology
Mr. Christian Jordan O.
dela Rosa
MS Biology
Maria Auxilia T. Siringan: As Reader and Examiner
Title or Topic of Thesis
Unit in
UPD
Isolation, Characterization and
identification of lactic acid bacteria from
traditional fermented vegetable
products of Bulacan
Successfully defended the thesis proposal
on April 13, 2010
Molecular Studies on Associated
bacterial communities of selected HABs
species in the Philippines
Successfully defended the thesis proposal
in February 2010
Influence of salinity on the infectivity of
Vibrio parahaemolyticus in Molobiscus
(Oreochromis niloticus x Oreochromis
mossambicus)
Thesis in progress
Phenotypic and Genotypic
Characterization of Facultative
Methylotrophic Bacterial Isolates from
Philippine Shrimp Paste
(Bago-ong)
Successfully defended the thesis
proposal on August 27, 2010
Adviser(s)
Dept. of
Biology,
CS, DLSU
Dr. Marian P. De
Leon
MSI, CS,
UPD
Dr. Rhododra V.
Azanza
IB, CS,
UPD
Dr. Zubaida Basiao
IB, CS,
UPD
Dr. Windell L. Rivera
74
cont. of table 5.
Name of Student and
Degree
Maria Auxilia T. Siringan: As Reader and Examiner
Title or Topic of Thesis
Unit in
UPD
Ms. Angeles de Leon
MS Microbiology
Macrofungi Utilized by Aeta
Communitiesin Selected Areas of Central
Luzon: Systematics and Ecology,
Taxonomy, Molecular Phylogeny and
Tissue Culture for Mass Cultivation
Successfully defended the thesis proposal
on November 23, 2010
Table 4b. Vina B. Argayosa: As Co-Adviser
Rubie Ganzon
Characterization of Unknown Alkaliphilic
BS Biology (4th year)
bacteria found in Manleluag,
Pangasinan Hot Spring and Screening for
Selected Enzymatic Activity
Thesis in progress
Graduate
School,
UST
IB, CS
Table 6. Theses and investigatory projects assisted by the MRSL in 2010.
Topics of Theses or Investigatory Projects
Academic Institution
Tertiary Level
Ateneo de Manila
Percent kill of hand sanitizer against A. niger
University
Heterotrophic plate count of water samples
Colegio de San
Antimicorbial assay (AMA) of Alagao leaves extract
Agustin
against E. coli and S. aureus
Our Lady of Fatima
AMA of Rhizophora apiculata leaf extract against E.
University
coli and S. aureus
Polytechnic University
of the Philippines
University of the East
Ramon
Magsaysay Medical
Center
University of the
Philippines
AMA of banana peel extract against E. coli and S.
aureus
AMA of extract against E. coli and S. aureus
AMA of plant extract against C. albicans and T.
mentagrophytes
Microbial load of drinking glasses using swab method
AMA of plant extract against B. subtilis, E. coli and S.
aureus
AMA of barak root extract against B. subtilis and C.
albicans
AMA of latundan, saba, lakatan against C. albicans
AMA of ampalaya capsule against E. coli
L-drying of microbial cultures
AMA of zinc, cobalt, iron and copper against E. coli
and S. aureus
AMA of sample against S. aureus, E. coli and C.
albicans
Minimum inhibitory concentration of 4'carboxychalcone, 4'-hydroxychalcone and 4methoxychalcone against M. luteus
AMA of malunggay extract vs. E. coli, P. aeruginosa,
B. subtilis
Minimum inhibitory concentration of 4Hydroxychalcone against M. luteus
Adviser(s)
Dr. Thomas Edison E.
Dela Cruz
Dr. Anacleto M.
Argayosa
Name of Student or
Adviser
Ellison Ng
Vimischa Balazo
Gabriel A. Jimenez
Sheila Marie M.
Celso
Clarissa Rivera
Bernadeth Darasan
Lorna Enerva
Rachel Joyce M.
Agliam
Dr. Lorna T. Enerva
Bernadette G.
Daracan
Henry D. Mina
Nikko Paolo R.
Cablao
Prof. Marilen M.
Parungao
Valeree Ross R.
Bernardo
Charmaine
Alcantara
Roberto O.
Katigbak
Mary Rose Galeno
Patricia Mae B.
Mariano
75
cont. of table 6.
Topics of Theses or Investigatory Projects
Academic Institution
University of Rizal
System
Tertiary Level
Minimum inhibitory concentration of FC6, 1000 ppm
solution in 50% ethanol
AMA of samples against E. coli, S. aureus, P.
aeruginosa, M. luteus, C. albicans
AMA of untreated and untreated ZnO nanoparticle
against E. coli and S. aureus
AMA of acyclic diamide against E. coli, S. aureus, C.
albicans and A. niger
Sinandomeng and super angelica against E. coli
AMA of turmeric extract against T. mentagrophytes
AMA of malunggay leaf extract against S. aureus
AMA of malunggay extract against S. aureus
AMA of sample against S. typhimurium
University of Santo
Tomas
L-drying of microbial cultures
Confirmation of identification of bacterial isolate
University of St. Louis
AMA of sample against S. aureus
Secondary Level
Evaluation of antimicrobial Activity and
phytochemical screening of Syzygium samarangense
AMA of saluyot crude extract against E. coli
Araullo High School
Name of Student or
Adviser
Djanna Cornago
Maxilmilien Alaric O.
Tan
Rochelle Anne Tan
Graniel Heine A.
Abrenica
Ivy Ann Menese
March Sherwin C.
Loveria
Natalie Bernadette
G. Amonoy
Jennie C. Manimtim
Kathreen B.
Razonable
Dr. Gina R. Dedeles
Manuelito L.
Barredo
Ranchie Ting
Ivan Cyrus Dalde
Vince John O.
Navarro
Myka Pescasio
Balara High School
Extract from button mushroom (Agaricus bisporus) as
antibacterial agent against E. coli
AMA of acapulco and ginger extract against S.
aureus
AMA of pansit-pansitan extract against E. coli
Capiz National High
School
AMA of milkfish serum against A. hydrophila, E. coli, P.
aeruginosa, K. pneumoniae, M. luteus, C. albicans
Cayetano Arellano
High School
AMA of makopa leaves, bark and root against S.
aureus
AMA of bermuda grass extract against K.
pneumoniae
AMA of alagao leaves extract against E. coli and S.
aureus
AMA of pure dalandan extract against C. albicans
Emelie R. Francisco
AMA of mangosteen extract against S. aureus
Jonas Arvin S.
Antonio
Malt Christian V.
Resuello
Christine Joy M.
Cayubit
Christelle Mae A.
Posadas
Stephanie Ann L
Bago
Robert Jade Jariel
Gianna Laurel S.
Ruizol
Roselyn V. Palco
Assumption Antipolo
Colegio de San
Agustin
Ernesto Rondo High
School
First City Providential
College
AMA of potato extract against S. aureus
AMA of kamias fruit extract against S. typhimurium
AMA of sayote plant against C. albicans
AMA of Euphorbia hirta against B. subtilis
AMA of sample against B. subtilis
AMA of tomato extract against P. aeruginosa
Francisco P. Felix
Memorial National
High School
AMA of Ipomoea batatas leaves against E. coli and S.
typhimurium
Jemminah Fajardo
Kevin Conrad G.
Angeles
Angeli Joyce Yap
Dy
Gabriel A. Jimenez
Alijay O. Daliwan
76
cont. of table 6.
Topics of Theses or Investigatory
Projects
Name of Student or Adviser
Tertiary Level
The feasibility study of using apple cider vinegar
Clarissa Ting
additive to facial cleanser
The feasibility of oregano extract as ani of E. coli
Unreported
Feasibility of Using thymol extract from thyme as main
Erica Chan
ingredient in toilet bowl disinfectant
The feasibility study of using oxidized β-carotene as a
Beatrix Garcia
replacement for tretinoin combating against S. aureus
The extract of chrysanthemum (Chrysanthemum
Daphne Yang
Cinerariaflorlium) and Its viability to inhibit the growth
on Staphylococcus aureus
The feasibility of madre de cacao (Gliricidia Septium)
Unreported
leaf extract as treatment for Trichophyton rubrum
Academic Institution
Immaculate
Conception Academy
La Salle Greenhills
Lakandula High School
Marist School
Quezon City Science
High School
Quezon City Science
High School
AMA of samples againsty E. coli, S. aureus, P. aeruginosa, C.
albicans
AMA of homemade soap and safeguard against S. aureus
Louise Marie Reyes
Effectiveness of garlic in fighting E. coli
Effectiveness of malunggay leaf extract as potential
Antiseptic
AMA of radish and gumamela against E. coli
Jawish Jadwani
Dean Matthew
Santos
Esmeralda de los
Santos
Christian Allen C.
Abad
Ruth Eliel Remorca
AMA of Pond's acne expert and Master facial wash
against E. coli
Antimicrobial properties of acapulco (Cassia alata)
Aqueous Extract on E. coli and S. aureus
AMA of janitor fish epidermal mucus against E. coli
and S. aureus
AMA of garlic crude extract against E. coli
AMA of radish and cabbage extract against C.
albicans
AMA of lemongrass extract against S. aureus
AMA of atis and guyabano extract against A. niger
AMA of mushroom extract against E. coli
Tridax procumbens pure extract against T.
mentagrophytes
Carageenan against B. cereus
AMA of sugarcane against S. aureus
AMA of liquid soap with lemon grass and guava
extract against S. aureus
AMA of kakawate extract against S. aureus
AMA of acapulco and sambong extract against S.
aureus and E. coli
AMA of janitor fish epidermal mucus against E. coli
and S. aureus
AMA of guava extract against S. aureus
May Antonette M.
Palacio
Roselyn S. Tisado
Hannah Foliente
Iris Salonga
Amiel Marc F.
Domingo
Bryan Joseph F.
Echauz
Justin Paul M.
Mijares
Joana Buag
Alexandra Lui N.
Villafuerte
Luigi Montevirgen
Amelyn Samonte
Ruth Eliel Y.
Remorca
Jethro Lee
Ryan Albert G.
Antonio
Marly Mae S.
Tabora
Ramon Magsaysay
High School
AMA of basil extract against S. aureus
Regional Science High
School
Rizal National High
School
AMA of hand sanitizer against S. aureus
Algene E. Murillo
AMA of ginger extract against E. coli, S. aureus, and S.
typhimurium
AMA of pandan extract against S. aureus
Lawrence Angel M.
Malasa
Vernalyn M. de
Leon
Gabrielle Justin S.
Dizon
AMA of acapulko and gugo antidandruff shampoo
against C. albicans
77
cont. of table 6.
Academic Institution
San Miguel National
High School
Topics of Theses or Investigatory Projects
Tertiary Level
AMA of sampaguita & ilang-ilang extract against E.
coli and S. aureus
St. Theresa's College
AMA of milkfish bile against P. aeruginosa
Tanza National High
School
AMA of peel and seed of lanzones extract against C.
albicans
AMA of guava extract against S. typhimurium
Percent kill of hand sanitizer against S. aureus
AMA of lanzones extract against C. albicans
Valenzuela City
Science High School
AMA of petroleum jelly + cashew nutshell against S.
aureus
AMA of aratiles and calamansi extract against S.
typhimurium
Name of Student or
Adviser
Laura Ashley
Manuzon
Reizelle Marie O.
Del Moral
Kamilah Lean Torres
Athena M.
Villagonzalo
Lariza D. Dulay
Frances Ann J.
Salcedo
Zarah Chrisitne P.
Cabalagnan
Lysharmaine Alaan
Support to Researchers
MRSL supported the research project of Dr. Raul V. Destura on the development of a
PCR-based kit for dengue detection. The research made use of the L-drying equipment to
prepare a prototype of the kit.
Linkages
International
•
World Federation of Culture Collections (WFCC)
UPCC is a member of the World Federation of Culture Collections (WFCC).
•
American Society for Microbiology (ASM)
Dr. Siringan is a voting member of the American Society for Microbiology (ASM)
under Division Q (Environmental Microbiology).
•
Southeast Asian (SEA) Sub Commission of the International Commission on
Microbiological Specifications for Foods (ICMSF).
•
Dr. Siringan is the Philippine representative in the Southeast Asian (SEA) Sub
Commission of the International Commission on Microbiological Specifications for
Foods (ICMSF).
National
•
National Codex Alimentarius Organization
Ms. Vina B. Orgayosa is a member of the Sub-Committee on Food Hygiene (SCFH),
Ad Hoc Working Group for the Drafting of the National Codex Organization Manual
of Operations, convened by the Department of Health (DOH).
•
Philippine Network of Microbial Culture Collection (PNMCC)
The University of the Philippines Natural Sciences Research Institute Culture
Collection (UPCC) of the MRSL is an affiliate member of the Philippine Network of
78
Microbial Culture Collection (PNMCC). UPCC is represented by Dr. Siringan who
served as Business Manager in 2010.
•
Philippine Society for Microbiology, Inc. (PSM)
Dr. Siringan is an active life-member of the Philippine Society for Microbiology, Inc.
(PSM). She is a Diplomate and one of the Examiners of the Philippine Academy of
Microbiology (PAM), the accreditation arm of the PSM.
Ms. Argayosa, Ms. Bulaong and Ms. Cereno are active life-members of the Society.
Ms. Argayosa and Ms. Bulaong are Specialist Microbiologists under the PAM
accreditation scheme.
Trainings conducted
1)
2)
Title of Training
:
Training Course on Bacteriological Examination of Water.
Funding Source
Date
Lecturer
Trainors
Fees
No. of participants
Profile of participants
:
:
:
:
:
:
:
MRSL and Training Fees
March 10 – 14 and August 10 – 14, 2010
Dr. Maria Auxilia T. Siringan
VB Argayosa, SSp Bulaong and CA Cereno
PhP 8,500 per person
10
Laboratory personnel/analysts from Water Districts (3), analyst
from the industry (1), analysts from government agencies (5)
and educator (1)
Title of Training
:
Training Course on Bacteriological Examination of Water.
Funding Source
Date
Lecturer
Trainors/Facilitator
Fees
No. of participants
Profile of participants
:
:
:
:
:
:
:
MRSL and Training Fees
September 20 – 24, 2010
Dr. Maria Auxilia T. Siringan
VB Argayosa, SSp Bulaong and CA Cereno
PhP 8, 500.00 per person
9
Laboratory personnel/analysts from Water District (1), analysts
from the industry (4) and analyst from government agency (4)
MRSL Photo Gallery
Fig. 29a
Fig. 29b
MRSL conducted the training course on “Bacteriological Examination of Water” on March 10 to 14 and
September 20 to 24, 2010. Shown here is the second batch of 2010 trainees (Fig.29a). The participants
performed hands-on laboratory exercises. Shown in Fig. 29b is Ms. Argayosa (standing) supervising the
trainees in their laboratory exercises.
79
MRSL Researchers (L-R), Ms. Argayosa, (Fig.30a),
Ms. Bulaong (Fig.30b), and Ms. Cereno (Fig.30c)
participated in the 2010 Philippine MSTQ, Inc.
National
Microbiological
Proficiency
Testing
Program for Food Matrix, Non-pathogens category,
in September 2010.
The MRSL researchers
demonstrated
their
competence
in
the
enumeration of eight (8) microbial parameters,
under the non-pathogen category, namely,
Aerobic Plate Count, Bacillus cereus Count,
Coliform Count, Fecal Coliform Count, E. coli
Count, Lactic Acid Bacteria Count, Mold Count
and Yeast Count.
Fig.30a
Fig.30b
Fig.30c
.
Fig.31a
Fig.30b
Fig.31b
Fig.31a and 31b. MRSL Researchers were trained to calibrate pipetors by Ms. Anita Chng, Thermo
Scientific Commercial Product Manager. The pipet calibration training was sponsored by the
Molave Trading on November 5, 2010 at the MRSL.
Fig.32a
Fig.32c
Fig.32b
Fig.32d
Focus Biotech and Diamed Traiding installed the Biolog Gen III Microbial Identification System at the MRSL.
The MRSL researchers and invited participants were trained in the use of the identification system by Ms.
Cherlin Ooi (Fig.31a) of Focus Biotech. Ms. Ooi gave a lecture on the principles of the carbon utilization
technology (Fig.32a) and demonstrated the protocol for the identification (Fig.32b). Ms Argayosa is shown
in Fig.32c, preparing the microorganism to be identified. Ms. Ooi explained the use of the software used in
microbial identification. (Fig.32d)
Manpower Development
Activities of MRSL Staff in 2010
1) MA. AUXILIA T. SIRINGAN (University Researcher IV)
Positions held
•
•
•
•
•
•
•
•
•
MRSL Head
Chairman and Biosafety Officer, NSRI Safety Committee
Member, College of Science REPS Committee
Associate member, National Research Council of the Philippines
Diplomate, Philippine Academy of Microbiology
Examiner, Philippine Academy of Microbiology
Life member, Philippine Society for Microbiology
Life Member, Philippine Association for the Advancement of Science (PHILAAS)
Business Manager, Philippine Network of Microbial Culture Collections
Seminars/Workshop/Trainings attended
•
Member, Organizing Committee. 10th Philippine Network of Microbial Culture
Collections, Inc. (PNMCC) Annual Symposium and General Assembly. “Linking
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Microbial Systematics with Biotechnology”, Thomas Aquinas Research Complex
Auditorium, University of Santo Tomas, España, Manila. November 13, 2010.
Coordinator and participant. MRSL In-house Training/Workshop on Biolog
Omnilog Gen III System for Microbial Identification, NSRI, UP Diliman. November
8-9, 2010.
Participant. Workshop on Pipet Calibration. NSRI, UP Diliman. November 5,
2010.
Participant. Seminar on “Technical Tools for Safe Meat and Meat Products,
NMIS Conference Room, NMIS Compound, Visayas Avenue, Quezon City.
October 20, 2010.
Participant. Seminar on Good Pipetting Practice and other Technologies,
Oakwood Joy Nostalg Hotel, Ortigas, Mandaluyong. October 12, 2010.
Trainor and Lecturer. Training Course on “Bacteriological Examination of
Water”, Microbiological Research and Services Laboratory, Natural Sciences
Research Institute, University of the Philippines, Diliman, Quezon City. March 104 and September 20-24, 2010.
Presentor. Water Quality Issues: Microbial Hazards. Water Forum: Our Mission:
Safeguarding Water Quality. Sulo Hotel. September 14, 2010.
Presentor. Impact of Climate Change on Marine Microbial Pathogens, Cluster1
Symposium of the Philippine Society for Microbiology, “Mitigating Impacts of
Climate Change and Infectious Disease”, Dr. Paz Adriano Hall of Miriam
College. September 11, 2010.
Participant. Seminar-Workshop on Total Quality Management (5S Program),
NSRI, UPD, Diliman, Quezon City. September 7, 2010.
Coordinator for MRSL’s Participation in the Proficiency Testing for Food, NonPathogen Category. Philippine Metrology, Standards, Testing and Quality
(MSTQ) and IFM Quality Services Pty, Ltd., Australia. August to September 2010.
Coordinator, Trainor and Lecturer.
Training Course on “Bacteriological
Examination of Water”, Microbiological Research and Services Laboratory,
Natural Sciences Research Institute, University of the Philippines, Diliman,
Quezon City. March 10-14 and September 20-24, 2010.
Participant. Merck Safety Summit, Diamond Hotel, Manila. August 6, 2010.
Member. Organizing Committee. 39th PSM Annual Convention and Scientific
Meeting, “Microbes at Work”, Hyatt Hotel and Casino Manila, Malate, Manila.
April 29-30. 2010.
Presentor. Poster paper entitled “Carbon Utilization and Temperature
Requirements of Pink Pigmented Facultative Methylotrophic (PPFM) Bacteria”,
co –authored by C. J. dela Rosa. 39th PSM Annual Convention and Scientific
Meeting, Avenue Plaza Hotel, Naga City, Camarines Sur. April 29-30, 2010.
Presentor. Poster paper entitled “Carbon Utilization and Temperature
Requirements of Pink Pigmented Facultative Methylotrophic (PPFM) Bacteria”,
co –authored by Dela Tonga, A.D, J. M. C. Lomanta, M. A. C. V. Relucio, K. B.
Cabalteja, A.F. Salvador, J.M. Cayme, G. P. Concepcion. 39h PSM Annual
Convention and Scientific Meeting, Avenue Plaza Hotel, Naga City. Camarines
Sur, April 29-30, 2010.
Presentor. Poster paper entitled “Carbon Utilization and Temperature
Requirements of Pink Pigmented Facultative Methylotrophic (PPFM) Bacteria”,
co –authored by Relucio, M. A. C., J. M. C. Lomanta Lomanta, A. D. dela
Tonga, K. B. Cabalteja, A. F. Salvador, J. M. Cayme, G. P. Concepcion. 39th
PSM Annual Convention and Scientific Meeting, Avenue Plaza Hotel, Naga
City, Camarines Sur. April 29-30, 2010.
Presentor. “Marine Microbial Pathogens: Responses to Thermal Stress“, 2nd
PSM Northern Luzon Regional Convention. Baluarte Wildlife Park, Vigan, Ilocos
Sur. March 4, 2010.
Member. Organizing Committee. Local Organizing Committee. UNESCO
Manila Conference on Capacity-Building in Life Science. Sofitel Hotel, Manila.
February 6, 2010.
82
•
•
Member. Technical Working Group on Water Purification Devices/drinking
Water Treatment Units. Bureau of Health Devices and Technology (BHDT),
Department of Health (DOH), Rizal Avenue, Manila. January 26 – February 23,
2010.
Member. Organizing Committee. PSM Cluster 2 Symposium, “Microbes in
Action, Microbes on the Go”. Emilio Aguinaldo College, Taft Avenue, Manila.
January 23, 2010.
2) SONIA SP. BULAONG (University Researcher II)
Positions held
•
•
•
•
•
•
•
•
•
Associate Member, National Research Council of the Philippines
Specialist Microbiologist, Philippine Academy for Microbiology
Life Member, Philippine Society for Microbiology
Life Member, Philippine Association for the Advancement of Science (PHILAAS)
Member, Philippine Association of Food Technologists, Inc.
Member, Mycological Society of the Philippines (MSP)
Member, Biology Teachers Association ( BIOTA)
Member, Phi Sigma Honor Society
Member, Women Association of Scientists in the Philippines (WASP)
Seminars/Workshop/Trainings attended
•
•
•
•
•
•
Participant. MRSL In-house Training/Workshop on Biolog Omnilog Gen III System
for Microbial Identification, NSRI, UP Diliman. November 8-9, 2010.
Participant. Workshop on Pipet Calibration. NSRI, UP Diliman. November 5,
2010.
Participant. Seminar on “ Technical Tools for Safe Meat and Meat Products,
NMIS Conference Room, NMIS Compound , Visayas Avenue, Quezon City.
October 20, 2010.
Trainor and Lecturer. Training Course on “Bacteriological Examination of
Water”, Microbiological Research and Services Laboratory, Natural Sciences
Research Institute, University of the Philippines, Diliman, Quezon City. March 1014 and September 20-24, 2010.
Participant. Seminar-Workshop on Total Quality Management (5S Program),
NSRI, UPD, Diliman, Quezon City. September 7, 2010.
Participant. Proficiency Testing for Food, Non-Pathogen Category. Philippine
Metrology, Standards, Testing and Quality (MSTQ) and IFM Quality Services Pty,
Ltd., Australia. August to September 2010.
3) VINA B. ARGAYOSA (University Researcher I)
Positions held
•
•
•
•
•
•
•
•
Specialist Microbiologist, Philippine Academy for Microbiology
Life Member, Philippine Society for Microbiology (PSM)
Life Member, Philippine Association for the Advancement of Science (PHILAAS)
Member, Biology Teachers Association (BIOTA)
Member, UP Housing Cooperative, Inc.
Member, Mycological Society of the Philippines
Member, Philippine Association of Food Technologists
Member, Women Association of Scientist of the Philippines (WASP)
Seminars/Workshop/Trainings attended
•
•
Participant. MRSL In-house Training/Workshop on Biolog Omnilog Gen III System
for Microbial Identification, NSRI, UP Diliman. November 8-9, 2010.
Participant. Workshop on Pipet Calibration, NSRI, UP Diliman. November 5, 2010.
83
•
•
•
•
Trainor and Lecturer. Training Course on “Bacteriological Examination of
Water”, Microbiological Research and Services Laboratory, Natural Sciences
Research Institute, University of the Philippines, Diliman, Quezon City. March 10
to 14 and September 20-24, 2010.
Participant. Seminar-Workshop on Total Quality Management (5S Program),
NSRI, UP Diliman, Quezon City. September 7, 2010.
Participant. Proficiency Testing for Food, Non-Pathogen Category. Philippine
Metrology, Standards, Testing and Quality (MSTQ) and IFM Quality Services Pty,
Ltd., Australia. August to September 2010.
Participant. Merck Safety Summit, Diamond Hotel, Manila. August 6, 2010.
4) CELIA A. CERENO (University Researcher I)
Positions held
•
•
•
•
•
Associate Member, National Research Council of the Philippines
Life Member, Philippine Microbiology Society (PSM)
Member, Philippine Association for the Advancement of Science (PHILAAS)
Member, Women Association of Scientist in the Philippines (WASP)
Member, Philippine Association of Food Technologists, Inc. (PAFT)
Seminars/Symposia/Trainings attended
•
•
•
•
•
•
•
Participant. 10th Philippine Network of Microbial Culture Collections, Inc.
(PNMCC) Annual Symposium and General Assembly.
“Linking Microbial
Systematics with Biotechnology”, Thomas Aquinas Research Complex
Auditorium, University of Santo Tomas, España, Manila. November 13, 2010.
Participant. MRSL In-house Training/Workshop on Biolog Omnilog Gen III System
for Microbial Identification, NSRI, UP Diliman. November 8-9, 2010.
Participant. Workshop on Pipet Calibration, NSRI, UP Diliman. November 5, 2010.
Trainor and Lecturer. Training Course on “Bacteriological Examination of
Water”, Microbiological Research and Services Laboratory, Natural Sciences
Research Institute, University of the Philippines, Diliman, Quezon City. March 1014 and September 20-24, 2010.
Participant. Seminar-Workshop on Total Quality Management (5S Program),
NSRI, UPD, Diliman, Quezon City. September 7, 2010.
Participant. Proficiency Testing for Food, Non-Pathogen Category. Philippine
Metrology, Standards, Testing and Quality (MSTQ) and IFM Quality Services Pty,
Ltd., Australia. August to September 2010.
Participant. Merck Safety Summit, Diamond Hotel, Manila. August 6, 2010.
RESEARCH AND ANALYTICAL SERVICES LABORATORY (RASL)
The Research and Analytical Services Laboratory (RASL) renders chemical analytical
services to students, researchers, entrepreneurs, industries and government agencies. RASL
holds the distinction of the first ISO 17025:2005 – Accredited Laboratory in the UP System. RASL
conducts research on validation and development of methods and in generation of baseline
information on toxic trace metals and trace organics in the Philippine environment. In addition
to analytical services, RASL conducts training courses for chemists to upgrade their technical
capability to do quality chemical analyses.
Extension Services/Clients
Analytical Services
Customers Served by RASL in 2010
Note:
Type I
Type II
Type III
Type IV
UP College of Science Students
UP NSRI Researches
UP Diliman Students outside of the College of Science
UP-Funded Researches outside NSRI and Students of
UP outside Diliman
Type I
(5,
1.68 %)
Type II
(5,
1.68 %)
Type III
(7,
2.35 %)
Type IV
(4,
1.34 %)
Type V
(257,
86.24 %)
Type VI
(16,
PT
(2,
0.67 %)
5.37 %)
Cancelled*
(2,
0.67 %)
Type V Government, Small and Medium Scale Industries, and
Private Institutions
Type VI Multinationals and Big Companies (Top 1000)
PT
Proficiency Testing
Types Services Rendered in 2010
Note:
AAS – Atomic Absorption Spectrophotometry
ASV – Anodic Stripping Voltammetry
GC/MS – Gas Chromatography / Mass Spectrometry
AAS Flame
(579) 38.09 %
AAS Cold Vapor
(254)
16.71 %
AAS Hydride Generation
(18)
1.18 %
ASV
(59)
3.88 %
GC/MS
(101) 6.64 %
IC
(62)
Electrometric, glass electrode
(34)
4.08 %
2.24 %
Electrometric, ISE
(23)
1.51 %
Colorimetric
(156)
10.26 %
Gravimetric
(49)
3.22 %
Titrimetric
(9)
0.59 %
TOC Analysis
(150)
9.87 %
Hardness
(26)
1.71 %
IC – Ion Chromatography
TOC – Total Organic Carbon
Support to Students
RASL supports students and researchers by making available the facilities of the
laboratory and by providing the analytical services that they need (Table 7 and Table 8).
Table 7. List of RASL’s users of facilities in 2010.
Name /
Project Leader
Facilities Used
School / University
Title of Research / Project
Meryl Antonio-Reyes
/ Dr. Anacleto
Argayosa
Ultralow Freezer
Institute of Biology /
Institue of Biology UP
Diliman, Quezon City
Jeysee Christine C.
Hong /
Ms. May Roxanne
Galapan Timbol
Ms. Maria Isabel
Beatrice R. Maza /
Ms. May Roxanne A.
Galapo-Timbol
Ms. Ginalyn Bacerdo
/ Prof. Cecelia
Cainto
Ms. Marie Joshua T.
Tapas /
Mr. Jeffrey
Venezuela
Uv-Vis
Spectrophotometer
UP College Home
Economics, UP Diliman,
Quezon City
Uv-Vis
Spectrophotometer
UP College Home
Economics, UP Diliman,
Quezon City
Cashew-Apple Antioxidants
Uv-Vis
Spectrophotometer
Polytechnic University of
the Philippines
Nitrate Determination in Baby
Foods for 4-6 Months Old
Centrifuge
Photocatalytic Degradation of
Methyl Orange Under
Monoporous Cement
Supported TiO2 Nanotubes
Ms. Loida O.
Casalme /
Dr. Florentino Sumera
Ultralow Freezer
UP College of
Engineering,
Department of Mining,
Metallurgical and
Materials Engineering,
UP Diliman, Quezon City
Institute of Chemistry,
UP Diliman, Quezon City
Ms. April Anne B.
Hachero /
Dr. Carina S. Ramos
SPE Manifold
Ultralow Freezer
Institute of Chemistry /
UP Natural Sciences
Research Institute, UP
Diliman, Quezon City
Ms. Arianne
Jabonero and Ms.
Krizhia Ip /
Mr. Jeffrey
Venezuela
Centrifuge
Ms. Janelle Boncan /
Mr. Jeffrey
Venezuela
Centrifuge
Ms. Kristia May Marte
/ Mr. Artemio E.
Pascual
Dr. Ruel M. Mojica
c/o Mr. Rio Carlo M.
Lirag and Ms. April
Anne B. Hachero
Ms. Noemi Pagdigan
/ Dr. Christine
Hernandez
Ms. Lilibeth Cajuday
/ Dr. Florentino
Sumera
Uv-Vis
Spectrophotometer
UP College of
Engineering,
Department of Mining,
Metallurgical and
Materials Engineering,
UP Diliman, Quezon City
UP College of
Engineering,
Department of Mining,
Metallurgical and
Materials Engineering,
UP Diliman, Quezon City
UP College Home
Economics, UP Diliman,
Quezon City
DA-BAR and UP Natural
Sciences Research
Institute, UP Diliman,
Quezon City
Institute of Chemistry,
UP Diliman, Quezon City
Rotary Evaporator
Uv-Vis
Spectrophotometer
Ultralow Freezer
Ultralow Freezer
Institute of Biology, UP
Diliman, Quezon City
Affinity Chromatography,
Molecular Weight Analysis and
Agglutination Assay from the
Gut of Oreochromis Niloticus
Vitamin C Determination of
Different Guava Jam
Formulation
Synthesis and Characterization
of Polyurethane Polymers
Based on (ω-1)-Coconut FAs
Copolymerized with Simple
Polyols and Hydroxyacids
Toxicological Assessment of
Extracts of Indoor Dust,
Laboratory and Faculty Room
Air Samples and Diesel /
Biodiesel Exhaust-Impacted Air
Using Allium cepa Cytogenetic
Assay
A Comparative Study Between
Degussa P-25 and Anataserutile TiO2 Mixture Under
Varying Conditions of Light
Photocatalytic Activity of
Titanium (IV) Dioxide
FS 215 Class
Influence of Roasting on
Phenolic Content and
Antioxidant Activity of
Philippine Coffee
Potential Anti-asthma
Compounds from Selected
Philippine Plants.
Developmental Competence
and Midkine Expression of
Philippine Water Buffalo
oocytes in Media
Supplemented with RA During
IVM
86
Table 8. List of students and researchers who requested assistance for thesis, research and class projects.
Assistance for
Name
Degree / Course
School /
Title
Thesis /
University
Research and
Class projects
Ms. Isavelina
BS Biology
UP Baguio,
Relationship of Organic
Analysis of Nitrite
Francesca A.
Biology
and Inorganic Pollutants
in Water Sample
Capala
Department
of Water and Metazoan
Gill Parasites of Nile Tilapia
in Five (5) Fish Ponds in
Isabela
Dr. Carina
Research Project Institute of
Toxicological Assessment
Analysis of
Ramos c/o
Chemistry / UP
of Extracts of Indoor Dust,
Polycyclic
April Anne
Natural Sciences
Laboratory and Faculty
Aromatic
Hachero
Research
Room Air Samples and
Hydrocarbons in
Institute, UP
Diesel / Biodiesel ExhaustBiodiesel
Diliman
Impacted Air Using Allium Exhaust Extract
cepa Cytogenetic Assay
Henry I.
Research Project ADSC-ABPD, UP
Applied Animal
Analysis of
Rivero
Los Baños
Biotechnology for the
Cadmium and
Improvement of
Organochlorine
Philippine Mallard
Pesticides in
Duck Eggs
Samples
Mr. Laurence
Research Project UP Manila,
Analysis on the Possible
Analysis of
Daquigan
Department of
Sources of arsenic in
Arsenic in Water
Physical Sciences
Local Water Farms in
Samples
and Mathematics Zambales
Dr. Florentino
Research Project UP Natural
Synthesis and
Analysis of Fatty
Sumera c/o
Sciences
Characterization of
Acid Methyl
Ms. Loida
Research
Polyurethane Polymers
Esters of
Casalme
Institute, UP
Based on (ω-1)-Coconut
Synthetic
Diliman
FAs Copolymerized with
Product
Simple Polyols and
Hydroxyacids
Mr. Rob H.
Undergraduate
National Institute
Trace Metal Detection in
Analysis of
Entac
Thesis
of Physics, UP
the Water from Marikina
Cadmium,
Diliman
River
Calcium,
Chromium,
Copper, Iron,
Magnesium,
Manganese,
Lead, Nickel,
Potassium,
Sodium, Zinc
and Mercury in
Water Samples
Mr. Rob H.
Undergraduate
National Institute
Trace Metal Detection in
Analysis of
Entac
Thesis
of Physics, UP
the Water from Marikina
Cadmium,
Diliman
River
Calcium,
Chromium,
Copper, Iron,
Magnesium,
Manganese,
Potassium,
Sodium and Zinc
in Fish Sample
Mr. Ralph S.
MS Thesis
Institute of
Isolation, Characterization Analysis of
Hipolito
Biology, UP
and Determination of
Phenols and
Diliman, Quezon
Phenol Degrading Ability
Catechols in
City
of Bacteria from Soil and
Extract Samples
Sediment Samples
87
cont. of table 8.
Name
Degree / Course
Ms. Joycelyn
C. Jumawan
Research Project
Institute of
Biology, UP
Diliman, Quezon
City
Food Science
and Nutrition
FS 215 c/o
Ms. Kristia
May Marte
FS 215 Class
UP College of
Home Economics,
UP Diliman,
Quezon City
Ms. Arianne
C. Jabonero
/
Ms. Janelle
Boncan
Undergraduate
Thesis
Ms. Kamina
Cheenee G.
Medallon
Undergraduate
Thesis
UP College of
Engineering,
Department of
Mining,
Metallurgical and
Materials
Engineering, UP
Diliman, Quezon
City
DAC, UP Manila
Ms. Lelibeth
Alveza
Mr. Rendell H.
Tiay
Undergraduate
Thesis
School /
University
UP Natural
Sciences
Research
Institute, UP
Diliman
UP College of
Engineering,
Department of
Mining,
Metallurgical and
Materials
Engineering, UP
Diliman, Quezon
City
Title
Toxicity of Sub-Acute
Lead Chloride to Sucker
Mouth Armored Catfish
pterysoplicthys pardalis:
Bioaccumulation, Organ
Pathologies and Other
Physiological Effects
Photocatalytic Effect of
Fe-doped Titanium
Nanotubes on Methyl
Orange Degradation
Assistance for
Thesis /
Research and
Class projects
Analysis of Lead
in Treated Water
Sample
Analysis of
Calcium and
Magnesium in
Prepared
Solutions and UV
Reading of
Prepared
Solutions
UV Reading of
Prepared
Solutions
An Analysis of the Water
Content and Conditions
of the Life Forms in Manila
Bay (Cavite Part)
Personal
Analysis of
Mercury in
Seawater and
Fish Samples
Analysis of pH of
Water Sample
Reduction of Chloroform
Content Using Ni-doped
Titania Pellets
UV Reading of
Prepared
Solutions
Consultation of students
1. Consultation of Isabella Pamintuan, UP Manila College of Medicine student
regarding analysis of phosphate in anaerobic treatment of waste for her masteral
thesis.
2. Consultation of Ralph Hipolito of UP Diliman, Institute of Biology on the analysis of
phenols and cathecols for his masteral thesis.
88
Linkages
Local
•
Environmental Management Bureau, Department of Environment and Natural
Resources
Joint Sampling of Air for the East Asia POPs Air monitoring Project
•
Philippine Metrology, Standards, Testing, and Quality (MSTQ)
Active participation as board member in pursuing the goal of the association in
enhancing the infrastructure for quality in the country
•
University of the Philippines in the Visayas, On - the job training of Chemistry
Students in the laboratory
•
National Codex Organization, Department of Agriculture- participation as
member of the Sub Cmmittee on Methods of Analysis and Sampling and Sub
Committee on Fats and Oil
International / Foreign
•
United Nations University, Tokyo, Japan
- Active participation as the implementor in the country of the regional
research program of UNU in Monitoring POPs in the East Asian Region
•
United Nations Environment Program.
- As consultant to EMB on matters regarding the Global Monitoring
Program of UNEP for the Stockholm Convention.
•
Ministry of Environment of Japan for POPs in East Asia Project
- Active participation in the regional project POPs in Air in East Asia to
generate baseline data for the assessment of the effectivity of the
Stockholm Convention.
•
Environment Canada Air Quality Processes Research Division
- As collaborator in sampling for the Global Air Passive Sampling project.
Trainings conducted
RASL has developed three training courses to help laboratories prepare for the
technical requirements of ISO-17025. These are Quality Assurance in Chemical Analysis,
Validation of Chemical Analytical Methods and Measurement of Uncertainty in Chemical
Analysis. These courses are offered every year. In addition to the regular training courses, RASL
conducts special training as requested.
Regular training courses conducted in 2010
1.
Title of Training
:
Validation of Chemical Methods of Analysis
Funding Source(s)
Date
Trainor(s)
Fees
No. of participants
Profile of participants
:
:
:
:
:
:
Participants paid a fee for the training
March 11-12, 2010
Charita S. Kwan and Artemio E. Pascual
PhP 5,000.00/participant
27
Chemists from government agencies and private industries
89
Fig.33. Charita S. Kwan and Artemio E.
Pascual (Trainors), RASL staff with the
participants of Training Course on
“Validation of Chemical Methods of
Analysis” conducted on March 11-12,
2010.
2.
Title of Training
:
Quality Assurance in Chemical Analysis.
Funding Source(s)
Date conducted
Trainor(s)
Fees
No. of participants
Profile of participants
:
:
:
:
:
:
Participants paid a fee for the training
April 7-8, 2010
Dr. Evangeline C. Santiago and Ms. Criselda R. Africa
PhP 5,000.00/participant
27
Chemists from government agencies and private industries
Fig.34. Dr. Evangeline C. Santiago and
RASL staff with the participants on the
Training Course on “Quality Assurance
in Chemical Analysis” conducted on
April 7-8, 2010.
3.
Title of Training
:
Uncertainty of Measurement in Chemical Analysis
Funding Source(s)
Date conducted
Trainor(s)
Fees
No. of participants
Profile of participants
:
:
:
:
:
:
Participants paid a fee for the training
April 29-30, 2010
Dr. Evangeline C. Santiago
PhP 5,000.00/participant
21
Chemists from government agencies and private industries
Fig.35. Dr. Evangeline C. Santiago (Trainor)
with the participants of Training Course on
“Uncertainty of Measurement in Chemical
Analysis” conducted on April 29-30, 2010
4.
Title of Training
:
Analysis of Water and Fish by Flame Atomic Absorption
Spectrophotometry
Funding Source(s)
Date conducted
Trainor(s)
Fees
No. of participants
Profile of participants
:
:
:
:
:
:
Participants paid a fee for the training
November 10, 11 & 12, 2010
Artemio E. Pascual and Criselda R. Africa
PhP 12,000.00/participant
11
Participants mostly from Department of Science and
Technology (DOST) from different regions
Fig.36. Mr. Artemio Pascual (Trainor) with
the participants of Training Course on
“Analysis of Water and Fish by Flame
Atomic Absorption Spectrophotometry”
conducted on November 10, 11 & 12,
2010”
Recent developments and other related activities of RASL for 2010
1. Participated in two (2) Laboratory Proficiency Testing (PT) Programs:
a. Proficiency testing of one (1) water sample from Environmental Resource
Associates for the analysis of Cadmium, Chromium, Copper, Iron, Lead,
Manganese, Nickel, Zinc and Boron in Water Sample from April 21 – May 21, 2010.
b. Proficiency testing of one (1) water sample from Environmental Resource
Associates for the analysis of Fluoride in Water Sample from April 21 – May 15, 2010.
2. Conducted Internal Audit of RASL on September 29 to October 15, 2010 done by
Senior Researchers who has attended the training course on “ISO/IEC 17025:2005
Laboratory Internal Audit”.
3. Acquisition of Hydride Vapor Generator and Graphite Furnace for Atomic Absorption
Spectrophotometry thru National Science Complex Funding.
Visitors/Guests
Name of Visitor:
Date:
Nobel Peace Prize Awardee: Nobel Laureat Prof. Torsten Wiesel
January 27, 2010
Name of School: UP National Institute of Molecular Biology and Biotechnology, UP
Diliman, QC
No. of Visitors:
≈10 Students
Date:
February 10 & 17, 2010
Name of School: Nueva Vizcaya State University
No. of Visitors:
150 Students
Date:
August 26, 2010
Name of School: Philippine Science High School – Central Luzon Campus
No. of Visitors:
18 Students
Date:
November 18, 2010
Other activities for 2010
1. Team Building Activities of RASL held on May 19, 2010 at Montemar, Bagac, Bataan.
Fig.37. RASL Staff for the Team Building
Activity at Montemar Beach Resort,
Bagac, Bataan on May 19, 2010
(Photo taken at Mt. Samat in Bataan)
2. On-the-Job Training of Wilyn Joy A. De la Cruz and Dave Eldon B. Olano, B. S. Chemistry
students of University of the Philippines in the Visayas on April 12 to May 18, 2010.
Fig.38. RASL staff with Wilyn Joy A. De la
Cruz and Dave Eldon B. Olano, On-theJob Trainees, B. S. Chemistry students of
University of the Philippines in the Visayas
during the issuance of Certificate of
Completion on May 18, 2010
Manpower Development
Activities of RASL Staff in 2010
1) EVANGELINE C. SANTIAGO (University Researcher V)
Positions held
•
•
•
Head and Technical Manager, RASL
National Project Coordinator for the Philippines (1999-present), United Nations
University EDC Monitoring in the East Asian Hydrosphere
Member, Scientific Productivity System Committee, UP System, (January 2006 to
present).
Professional/Extension activities
•
•
•
•
•
•
•
•
•
Member. Expert Working Group – Trial Air Monitoring Project in East Asia for the
Global Monitoring Program of the Stockholm Convention, 2003-present.
Trainor-Lecturer. “Quality Assurance in Chemical Analysis”. UP NSRI, Diliman,
Quezon City. April 7-8, 2010.
Trainor-Lecturer. “Uncertainty of Measurement in Chemical Analysis”. UP NSRI,
Diliman, Quezon City. April 29-30, 2010.
Participated in meetings and on-line discussion of the National Codex
Organization Sub Committee on Methods of Analysis and Sampling
Participated in meetings and on-line discussion of the National Codex
Organization Sub Committee on Fats and Oils
Organized the seminar Technical Tool for Safe Meat Products for PhilMSTQ ijn
cooperation with National Meat Inspection Services (NMIS). October 20, 2010.
Participated in Round Table Discussion on Conformance and Standards of the
Chemistry Division of NRCP for the Consultation Workshop on Strengthening the
Philippines for ASEAN Competitiveness. November 12, 2010.
Conceptualized and directed the training course on Analysis of Trace Metals in
Water and Fish by AAS offered for the first time. November 10-12, 2010.
President. Philippine Metrology, Standards, Testing, and Quality, Inc. July, 2008present.
Seminars/Symposia/Trainings attended
•
•
•
Presentor. Country Report PCBs in Pasig River and Laguna Lake at the UNU
International Meeting. Tiruchirappalli, India. January 22, 2010.
Paper presentor. Organochlorine Pesticides in Ambient Air in Selected
Residential Areas in the Philippines Derived from Passive Samplers with
Polyurethane Foam Disks in the UNU International Symposium on Trace Organic
Pollutants in the Environment. Tiruchirappalli, India. January 23, 2010.
Participant. 8th Workshop on Environmental Monitoring of Persistent Organic
Pollutants (POPs) in East Asian. Yokohama, Japan. September 23-25, 2010.
Paper Published
•
“Organochlorine Pesticides in Ambient Air in Selected Residential Areas in the
Philippines Derived from Passive Samplers with Polyurethane Foam Disks” in
Bulletin of Environmental Contamination and Toxicology, Vol 86, No.1 pp 50-55.
Research Project Implemented
•
Monitoring of Polybrominated Diphenyl Ether (PBDE) in Sediments with funding
from the United Nations University.
93
2) CHARITA S. KWAN (University Researcher III)
Positions held
•
Deputy Administrative Manager and Analyst Supervisor, RASL
Professional/Extension activities
•
•
•
Trainor-Lecturer. “Validation of Chemical Methods of Analysis”, UP NSRI, Diliman,
Quezon City. March 11-12, 2010.
Member, Phil MSTQ, Inc
Fellow, JSPS-Ronpaku Ph.D. Dissertation Research Program for 2010. Laboratory
of Organic Geochemistry, Tokyo University of Agriculture and Technology under
Prof. Hideshige Takada. April 11 – July 9, 2010.
Seminars/Symposia/Trainings attended
•
•
•
Participant. “3rd Annual Food, Environment & Consumer Products Safety
Consortium” by Perkin Elmer, Isla Ballroom 3, Edsa Shangrila-La Manila. August
27, 2010.
Participant. Training Course on “ISO/IEC 17025:2005 Laboratory Internal Audit”
by Philippine Trade Training Center, Seminar Room C, 2nd Flr. PTTC Bldg. Sen. Gil
Puyat Ave., cor. Roxas Blvd., Pasay City. August 31–September 1, 2010.
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)” , UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
3) ARTEMIO E. PASCUAL (University Researcher II)
Positions held
•
•
Analyst Supervisor, RASL
Board Member – Kapisanan ng Kimika ng Pilipinas, Division of Analytical
Science, Philippines
Professional/Extension activities
•
•
•
Trainor-Lecturer. “Validation of Chemical Methods of Analysis, UP NSRI, Diliman,
Quezon City. March 11-12, 2010.
Trainor-Lecturer. “Analysis of Water and Fish by Atomic Absorption
Spectrophotometry”, UP NSRI, Diliman, Quezon City. November 10, 11 and 12,
2010.
Senior Lecturer I. UP College of Home Economics. June to October 2010.
Seminars/Symposia/Trainings attended
•
•
•
Participant. “25th Philippine Chemistry Congress” conducted by Integrated
Chemist of the Philippines, Subic Bay Exhibition & Convention Center, SBFPZ,
Olongapo City. April 8-10, 2010.
Participant. Training Course on “ISO/IEC 17025:2005 Laboratory Internal Audit”
conducted by Philippine Trade Training Center Seminar Room C, 2nd Flr. PTTC
Bldg. Sen. Gil Puyat Ave., cor. Roxas Blvd., Pasay City. 31 August – 1 September
2010.
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)”, UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
94
4) CRISELDA R. AFRICA (University Researcher I)
Position held
•
Analyst Supervisor, RASL
Professional/Extension activities
•
•
Trainor-Lecturer. “Quality Assurance in Chemical Analysis”, UP NSRI, Diliman,
Quezon City. April 7-8, 2010.
Trainor-Lecturer. “Analysis of Water and Fish by Atomic Absorption
Spectrophotometry”, UP NSRI, Diliman, Quezon City. November 10-12, 2010.
Seminars/Symposia/Trainings attended
•
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)”, UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
5) ABEGAIL Z. RASCO (University Research Associate II)
Position held
•
Analyst, RASL
Seminars/Symposia/Trainings attended
•
•
Trainee. “On the Job Training on Flame Atomic Absorption Spectrophotometry:
Potassium and Sodium”, Research and Analytical Services Laboratory, UP NSRI,
Diliman, Quezon City. October 4 –November 26, 2010.
Trainee. “On the Job Training on Ion Chromatography: Chloride, Nitrate,
Phosphate and Sulfate”, at the Research and Analytical Services Laboratory,
UP NSRI, Diliman, Quezon City. October – December 2010.
6) JENALYN V. CRUZ (Admistrative Aide VI)
Seminars/Symposia/Trainings attended
•
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)”, UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
7) JENNIFER L. RODRIGUEZ (Laboratory Technicial I)
•
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)”, UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
8) KRISTINE O. MANALANG (Analyst)
Seminars/Symposia/Trainings attended
•
•
Trainee. “On-the Job-Training on Total Organic Carbon in Water”, Research and
Analytical Services Laboratory, UP NSRI, Diliman, Quezon City. August –
December 2010.
Participant. “Milestone Microwave User’s Forum: Consolidation of Knowledge
and Experiences for Better Technology Innovations”, Guill-Bern Corporation,
Richmonde Hotel, Ortigas Center, Pasig City. June 22, 2010.
95
•
Participant. “3rd Annual Food, Environment & Consumer Products Safety
Consortium”, Perkin Elmer / Isla Ballroom 3, Edsa Shangrila-La Manila. August 27,
2010.
9) MARITESS NOELLE V. ARANCILLO (Analyst)
•
•
•
•
•
Participant. “NSRI Safety Management Seminar”, NSRI Conference Room, NSRI,
UP Diliman, Quezon City. January 25, 2010.
Participant. “Training Course on Validation of Chemical Methods of Analysis”,
Research and Analytical Services Laboratory, UP NSRI, Diliman, Quezon City.
March 11-12, 2010.
Participant. International Training Workshop on “Environmental Analysis of
PBDEs (Polybrominated Diphenyl Ether) in the Sediment”, United Nations
University and Shimadzu Corp., Shimadzu Sanjo Works, Kyoto, Japan. April 12-14,
2010.
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)”, UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
Participant. Training Course: “Passive Air Sampling: Long Range Transport of
Pollutants” Korea Ocean Research & Development Institute (KORDI) and United
Nations University in Tokyo, Japan. October 11-22, 2010.
10) CECILIA B. KOREA(Laboratory Aide)
Seminars/Symposia/Trainings attended
•
•
Trainee. “On-the Job-Training on Sample Preparation for Oil and Grease and
Petroleum Hydrocarbons in Water Samples by Gravimetric”, Research and
Analytical Services Laboratory, UP NSRI, Diliman, Quezon City. October 2009 –
February 2010.
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)”, UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
11) RAIAN F. LAPRESCA (Laboratory Aide)
Seminars/Symposia/Trainings attended
•
•
Trainee. “On-the Job-Training on Sample Preparation for Oil and Grease and
Petroleum Hydrocarbons in Water Samples by Gravimetric”, Research and
Analytical Services Laboratory, UP NSRI, Diliman, Quezon City. October 2009 –
February 2010.
Participant. Seminar-Workshop on “Total Quality/Workplace Management (5S
Program)”, UP Natural Sciences Research Institute Conference Room, UP NSRI,
Diliman, Quezon City. September 17, 2010.
2.2 INFORMATION SERVICE
The NSRI provides information on its technical services and research activities through
the following publications (for details, please see section 1.2).
1)
2)
3)
4)
The NSRI Research and Extension Services
The NSRI Catalog of Cultures
The NSRI Brochure
Yearly Announcement of Training Courses
96
2.3 COMMUNITY SERVICE
The NSRI as a national center of excellence in the basic sciences strives to promote
consciousness and appreciation for the natural sciences by hosting visits of students and
researchers and accommodating requests for demonstration of modern equipment for
various groups.
2.4 SAFETY PROGRAM
The NSRI continues to implement a Safety Program for the benefit of NSRI-based
researchers. The Safety Committee is chaired by Dr. Ma. Auxilia T. Siringan, who is the
Biological Safety Officer of the Institute. The Pollution Control Officer who implements the
Chemical Waste Management Program of the Institute is Ms. Leahfe P. Gabriel.
97
3. ORGANIZATION AND PERSONNEL
Organization and Management
The Organizational Chart of both regular and contractual personnel of NSRI is shown in
Figure 39. The various units, their principal personnel and functions are briefly described below:
Fig.39. The NSRI Organizational Chart
Executive Council
This is the highest policy-making authority within the NSRI consisting of the NSRI Director
and the Dean of the College of Science as Co-Chairpersons and the representatives of the
disciplinal and interdisciplinary units covered by the mandate of NSRI. The Executive Council
evaluates all research proposals and progress reports as well as other requests pertaining to
research.
The Director serves for a term of three years and is the chief executive of the Institute.
He/she communicates and implements the decisions of the Executive Council to parties
concerned, represents the Institute and/or negotiates collaborations in behalf of NSRI, initiates
programs and taps external sources of funding, both national and international.
The representatives are selected by their respective units for a term of two years. They
supervise the screening of research proposals within their units and act as liaison of their units
with the Executive Council.
The following were the members of the Executive Council in 2010:
Chairpersons:
Dr. Ernelea P. Cao
Director, NSRI
Dr. Caesar A. Saloma
Dean, College of Science
Members:
Dr. Zubaida U. Basiao (January 2008 – December 2010)
Representative, Institute of Biology
Dr. Leni L. Quirit (October 2008 – October 2010)
Dr. Guillermo M. Nuesca (November 2010 – present)
Representative, Institute of Chemistry
Dr. Jose Maria L. Escaner IV (October 2008 – present)
Representative, Institute of Mathematics
Dr. Lemmuel V. Aragones (June 2009 – present)
Representative, Institute of Environmental Science and Meteorology
Dr. Florentino C. Sumera (March 2009 – present)
Representative, Materials Science and Engineering Program
Research Section
This section performs the research functions of the NSRI. At present, most of the research
is undertaken through the faculty of the College of Science in the following disciplines: Biology,
Chemistry, Mathematics, and Environmental Sciences and Meteorology. The research thrusts
in these areas therefore reflect those of the institutes and departments. The members of NSRI’s
regular REPS who were initially hired to provide technical services to researchers were
recognized as a research unit in 1988 under the Research Policies and Guidelines of NSRI. As
such, the NSRI REPS have also been active in soliciting grants from NSRI. NSRI’s REPS have also
been active in obtaining external support for their projects (see section on Externally-Funded
Researches).
The research personnel of the NSRI thus consist of the following:
1. Project Leaders, Co-Project Leaders, Program Coordinators and Collaborators: faculty
researchers of the College of Science, NSRI research staff and adjunct researchers of the
College of Science.
2. Contractual Research Staff: University Research Associate I, hired on a contractual basis for
a particular project.
99
Technical Support Section
This section provides technical services for researchers and performs the extension
services described in Technical assistance and Advisory services section. The principal units
are the Biological Research and Services Laboratory (BRSL), the DNA Analysis Laboratory
(DAL), the Microbiological Research and Services Laboratory (MRSL) and the Research and
Analytical Services Laboratory (RASL).
Biological Research and Services Laboratory (BRSL)
1
1
1
University Researcher I
University Research Associate I
Admin. Aide IV
DNA Analytical Laboratory (DAL)
1
1
1
3
1
2
1
University Researcher III
University Researcher II (effective Jan. 21, 2010)
University Research Associate I (on study leave)
University Research Associate I (UP contractual)
University Research Associate I (substitute)
Research Associate (non-UP contractual)
Consultant
Microbiological Research and Services Laboratory (MRSL)
1
1
2
1
3
1
University Researcher IV
University Researcher II
University Researcher I
Laboratory Technician I
Laboratory Staff (non-UP contractual)
Consultant
Research and Analytical Services Laboratory (RASL)
1
1
1
1
1
1
1
4
University Researcher V
University Researcher III
University Researcher II
University Researcher I
University Research Associate II (effective Sept. 24, 2010)
Laboratory Technician I
Admin. Aide VI
Research and Analytical Staff (non-UP contractual)
Administrative Support Section
This section consists of the staff of the Administrative Office, Publication Section and the
Property Office:
1.
Administrative Office: handles the administration of research projects and actively
supports all activities of the in-house laboratories of the Institute. The specific functions
are: 1. To provide assistance to researchers in financial management, scheduling of
use of Institute facilities, provide support to clerical and artistic services in the
preparation of research manuscripts and coordinates with different laboratories
regarding their activities for research and extension, such as holding of seminars,
training courses, etc. and also, 2. To take charge of facilitating students and guests on
tour of the Institute. The staff consists of:
100
1
1
1
1
1
1
1
2.
Publication Section: offers printing, binding and photocopying services. It is equipped
with necessary facilities and manpower for the publication of research reports,
manuals, books, etc. The staff consists of:
1
1
3.
Publication Circulation Officer II
Admin. Assistant V
Property Section: in-charge of the overall maintenance and upkeep of NSRI building
and grounds, facilities and equipment, property and supply, administration of
electrical, plumbing and carpentry work. The staff consists of:
1
1
1
1
1
4.
Administrative Officer V
Administrative Officer IV
Administrative Officer III
Administrative Assistant II
Administrative Assistant II (Artist Illustrator II)
Admin. Aide IV
Admin. Aide III
Admin. Officer V
Precision Instrument Technician III
Admin. Assistant III
Laboratory Technician II
Laboratory Aide II
Computer Room:
provides for the computer needs of researchers, URAs and
administrative personnel, including e-mail and internet access through the DILNET.
List of NSRI Room Occupants in 2010
The complete list of NSRI’s room occupants in 2010 is shown in Table 9.
Table 9. Room Occupants of NSRI in 2010.
Chemistry Researchers occupying
rooms in NSRI
1. Dr. G. Nuesca
2. Dr. E. Llaguno
3. Dr. P. Espino
4. Dr. F. Del Mundo
5. Dr. C. Ramos
6. Dr. I. Rodriguez
7. Dr. F. Sumera
8. Dr. S. Arco
9. Dr. L. Quirit
10. Dr. L. Coo
11. Dr. F. Cariño/Dr. E.Amor
12. Dr. F. de Guzman
13. Dr. P.M. Sabido
Rm. 220
Rm. 215
Rm. 217
Rm. 221
Rm. 213
Rm. 222
Rm. 223
Rm. 226
Rm. 238
Rm. 219
Rm. 240
Rm. 242
Rm. 244
Biology Researchers occupying rooms in NSRI
1. Dr. A. Herrera/Dr. Z. Basiao
Rm. 115
2. Dr. G. Rivero/Dr. J. Puzon
3. Dr. E. Catap
4. Dr. E. Cao
5. Dr. L.M. Garcia
6. Dr. T. Salunga
7. Dr. W. Rivera
Rm. 116
Rm. 117
Rm. 118
Rm. 119
Snail Rm. 3
Snail Rms. 5,7,9
Animal House Rm. 7, 9
Animal House Rm. 11
8. Dr. A. Argayosa
NIP/IESM Researchers occupying rooms in NSRI
1. Dr. H. Ramos
2. Dr. D. Yanga
3. Dr. J. Magpantay
4. IESM Rooms
Rm. 243
Rm. 245
Rm. 246
Rm. 239 - 241
101
cont. of Table 9.
NSRI Researchers’ and Common Laboratory Rooms and Offices
1. DAL
Rms. 201-212, 214,216, 218
2. BRSL
3. MRSL
4. RASL
Rms. 120-127
Rms. 101-112, 114
Rms. 134-135, 138-139
& Pesticide Laboratory
Rm. 113
Rm. 234
Rm. 231
Rm. 137
5. Hot Room
6. Director/Admin Office
7. Conference/Council Rm
8. Property & Maint. Rm.
9. DA-BAR/UP-NSRI
Project
10. Computer Room
11. Storage Room
12. Visitor’s Room
Rm. 233
13. Animal House
14. Green House
15. General Room for
the Fume Hood
Rm. 15
Rm. 16
Rm. 236
Rm. 235
Rm. 237
Rm. 247
Personnel Inventory of the NSRI
The list of regular personnel of the NSRI in 2010 is presented in Table 10. The total
inventory of personnel for 2010 is provided in Table 11.
Table 10. Regular personnel of NSRI in 2010.
POSITION TITLE
Ernelea P. Cao
STATUS
OF
APPT.
Director
STATUS OF SERVICE
Additional Assignment
BIOLOGICAL RESEARCH AND SERVICES LABORATORY (BRS)
Raquel O. Rubio
University Researcher I
P
On active duty
Erna Louise L. Elias
Univ. Research Assoc. I
P
On active duty
Rhona A. Ong
Admin. Aide IV
P
On active duty
DNA ANALYSIS LABORATORY (DAL)
Maria Corazon A. De Ungria
Univ. Researcher III
P
On active duty
Gayvelline C. Calacal
Univ. Researcher II
P
On active duty, eff. Jan 21, 2010
Frederick C. Delfin
Univ. Research Assoc. I
P
On study leave
Jazelyn M. Salvador
Univ. Research Assoc. I
S
Subst. for Mr. Delfin eff. Sept. 4, 2009
Miriam Ruth M. Dalet
Univ. Research Assoc. I
C
UP Contractual
Sheena Marie B. Maiquilla
Univ. Research Assoc. I
C
UP Contractual
Minerva S. Sagum
Univ. Research Assoc. I
C
UP Contractual until Aug. 1, 2010
Maria Catherine B. Otero
Univ. Research Assoc. I
NC
Non-UP Contractual
Dorothy Emma C. Ferrer
Lab. Research Assoc.
NC
Non-UP Contractual
Saturnina C. Halos
Consultant
Additional assignment
MICROBIOLOGICAL RESEARCH AND SERVICES LABORATORY (MRSL)
Maria Auxilia T. Siringan
Univ. Researcher IV
P
On active duty
Sonia SP. Bulaong
Univ. Researcher II
P
On active duty
Celia A. Cereno
Univ. Researcher I
P
On active duty
Vina B. Argayosa
Univ. Researcher I
P
On active duty
Reynaldo E. Javate, Jr.
Laboratory Technician I
P
On active duty
Rosalyn F. Diaz
Laboratory Secretary
NC
Non-UP Contractual
Ma. Adel N. Arjona
Laboratory Assistant
NC
Non-UP Contractual
Dionisio R. Villanueva
Research Aide
NC
Non-UP Contractual
Luisa S. Saniel
Consultant
Additional Assignment
102
cont. of Table 10.
RESEARCH AND ANALYTICAL SERVICES LABORATORY (RASL)
Evangeline C. Santiago
Univ. Researcher V
P
On active duty
Charita S. Kwan
Univ. Researcher III
P
On active duty
Artemio E. Pascual
Univ. Researcher II
P
On active duty
Criselda R. Africa
Univ. Researcher I
P
On active duty
Abegail Z. Rasco
Univ. Research Assoc. II
P
On active duty, eff. Sept. 24,2010
Jennifer L. Rodriguez
Laboratory Technician I
P
On active duty
Jenalyn V. Cruz
Admin. Aide VI
P
On active duty
Maritess Noelle V. Arancillo
Analyst
NC
Non-UP Contractual
Kristine O. Manalang
Analyst
NC
Non-UP Contractual
Raian F. Lapresca
Laboratory Aide
NC
Non-UP Contractual
Cecilia B. Menguis
Laboratory Aide
NC
Non-UP Contractual
ADMINISTRATIVE SECTION
Leahfe P. Gabriel
Administrative Officer V
P
On active duty
Eleazar S. Del Remedio
Administrative Officer IV
P
On active duty
Lelibeth S. Alveza
Administrative Officer III
P
On active duty
Ernesto A. Canlas
Admin. Assistant II
P
On active duty
Edeza M. Rivera
Administrative Assistant II
P
On active duty
Reynaldo S. Teaño
Administrative Aide IV
P
On active duty
Romeo M. Magtibay
Administrative Aide III
P
On active duty
Jay Mathias A. Arellano
Project Dev’t Assoc. II
NC
Non-UP Contractual
Mark Joseph S. Santiago
Chem. Safety & Waste Mgt. Assoc.
NC
Non-UP Contractual
(Artist Illustrator II)
PUBLICATION SECTION
Vilma S. Vega
Publication Circulation Off. II
P
On-active duty
Florentino P. Abundo
Administrative Assistant V
P
On-active duty
PROPERTY SECTION
Leopoldo S. Nuñez
Administrative Officer V
P
On-active duty
Alvin DC. Mallari
Precision Instrument Tech. III
P
On-active duty
Lily Rose R. Dela Paz
Administrative Assistant III
P
On-active duty
Victoriano G. Bravo, Jr.
Laboratory Technician II
P
On-active duty
Edgardo C. Gayanes
Laboratory Aide II
P
On-active duty
Table 11. Personnel inventory of NSRI in 2010.
CATEGORY
No. of Personnel
(Man Years)
Regular Items
Lumpsum
TOTAL
NON-REGULAR
Members, Executive Council
Research Section
Project Leaders
Co-Project Leaders
Program Coordinators
Collaborators
University Res. Assoc. I
7
7
29
4
0
9
29
29
4
0
9
29
103
cont. of Table 11.
REGULAR
Director (Prof. VII)
1
1
1
1
2
3
4
1
2
1
2
1
1
1
2
3
4
1
2
1
2
1
2
1
1
1
1
1
1
2
1
2
1
2
1
1
1
1
1
1
2
1
2
1
-----------111
Research Staff
Univ. Researcher V
Univ. Researcher IV
Univ. Researcher III
Univ. Researcher II
Univ. Researcher I
Univ. Research Assoc. II
Univ. Research Assoc. I
Laboratory Aide II
Laboratory Technician I
Laboratory Technician II
Administrative Staff
Administrative Officer V
Publication Circulation Off. II
Administrative Officer IV
Administrative Officer III
Precision Instrument Tech. III
Administrative Assistant V
Administrative Assistant III
Administrative Assistant II
Administrative Aide VI
Administrative Aide IV
Administrative Aide III
TOTAL
104
3.1 NSRI STAFF DEVELOPMENT IN 2010
ERNELEA P. CAO (Director)
Positions held/Standing Committees and other assignments
•
•
•
•
•
•
•
•
•
•
Professor 9, Institute of Biology, College of Science, U. P. Diliman, Quezon City.
Affiliate Faculty Member of the UP Open University (June 1, 2000 – present).
Member, U.P. Diliman Institutional Biosafety Committee (UPDIBC) (1997 – present).
Member, Technical Panel for Biotechnology, Science and Technology
Coordinating Council, Philippine Council for Advanced Science and Technology
Research and Development (January 1, 2002 – present).
Member, Scientific and Technical Review Panel (STRP), Department of Agriculture
Member, Technical Committee of the Ecolabeling Programme (ELP) Board, Bureau
of Product Standards, Department of Trade and Industry (April 16, 2004 – present).
Member, University Council Committee on Student Organizations, Activities and
Welfare, (January 31, 2009 – January 30, 2012).
Member, Board of Directors for 2010-2012. Biotechnology Coalition of the
Philippines, Inc.
Founding member and Member of the Board of Directors for 2010-21012. Philippine
Society for Cell Biology, Inc.
Co-Secretary for 2010-2011, The International Honor Society of the Phi Kappa Phi,
UP Chapter 045.
Membership in Academic and Professional Organization
•
•
•
•
•
•
•
•
•
•
•
Biology Teachers’ Association of the Philippines, Inc.
Biotechnology Coalition of the Philippines, Inc. (Member of the Board for 2010-2012)
National Research Council of the Philippines (Regular Member – Division V)
Philippine Association for the Advancement of Science, Inc.
Philippine Association of University Women (U.P. Diliman Chapter)
Philippine Society for the Advancement of Genetics, Inc.
Philippine Society for Cell Biology (Founding Member and Member of the Board of
Directors 2010-2012)
Philippine Society for Microbiology, Inc. (Cluster II Chair - 2001-2002; Auditor – 20022003; Corresponding Secretary - 2003-2004; 2004-2005; Vice-President – 2005-2006;
President – 2006-2007; Immediate Past President (Ex-Officio) – 2007-2008)
Phi Sigma International Biological Honor Society (Alpha Chi Chapter)
The International Honor Society of the Phi Kappa Phi, UP Chapter 045
Women Association of Scientists in the Philippines, Inc.
Publications
•
•
•
De Guzman, M. C.and E. P. Cao. 2010. Cadmium binding ability of the blue-green
alga Hapalosiphon welwitschii Năgel under controlled conditions. Philippine
Science Letters 3 (1): 76 – 86.
Jalandoni-Buan, A. C., A. L. A. Decena-Soliven, E. P. Cao, V. L. Barraquio and W. L.
Barraquio. 2010. Characterization and identification of congo red decolorizing
bacteria from monocultures and consortia. Philippine Journal of Science 139 (1): 71
- 78.
Guzman, R. M. S., R. N. R. Gervasio, I. K. C. Fontanilla and E. P. Cao. 2010. Frequency
dstribution of blood groups ABO, MN and Rh factor in Philippine cosmopolitan,
regional, and the national populations. Science Diliman 21 (2): 43 – 49.
Conferences attended
•
Asia Pacific Conference on Insect Resistance Management (IRM) for Bt Crops, AIM
Conference Center, Makati City (Session Chair). March 18 – 19. 2010.
105
•
•
•
39th Annual Convention of the Philippine Society for Microbiology, Inc. on the
theme “Global Alert: Microbes at Work,” The Avenue Plaza Hotel, Naga City,
Manila, Philippines. April 29 – 30, 2010.
Seminar-Workshop on “Total Quality Management (5S Program),” NSRI Conference
Room, U. P. Diliman, Quezon City, conducted by Prof. Jorge V. Sibal, Dean. School
of Labor and Industrial Relations, U.P. Diliman, Quezon City. September 17, 2010.
Seminar on “Science and Regulatory Perspectives on Stacked Events in Genetically
Modified Crops,” September Hotel Borobudur, Jakarta, Indonesia, sponsored by the
International Life Sciences Institute, Southeast Asia Region. September 22 – 23, 2010.
Extension Services
•
•
•
Judge. Finals Round, Research Fair 2010: Breaking Ground, Transcending
Boundaries, College of Engineering, U.P. Diliman, Quezon City, sponsored by the
University of the Philippines Academic League of Chemical Engineering Students.
January 29-30, 2010.
Judge. Initial Evaluation for Finalists for Best Paper (Basic Research) at the 22nd DABAR National Research Symposium. September 2, 2010.
Judge. Final Evaluation, Best Paper (Basic Research) at the 22nd DA-BAR National
Research Symposium. October 7 – 8, 2010.
LEOPOLDO S. NUÑEZ (Administrative Officer V)
Additional assignments
•
•
•
Building Administrator, U.P. NSRI, Diliman, Q.C.
Member, NSRI Research and Administrative Committee (RAC)
Member, NSRI Administrative Personnel Committee (APC)
Seminars/Trainings attended
•
•
•
Resource Person. NSRI Safety Seminar, NSRI Conference Room, UP Campus,
Diliman, Quezon City. January 20, 2010.
Resource Person. NSRI Safety Seminar, NSRI Conference Room, UP Campus,
Diliman, Quezon City. July 2010.
Permits and Licenses:
Regulatory Requirements of Chemical Substances
Importation and Usage, Diamond Hotel, Manila. November 12, 2010
LEAHFE P. GABRIEL (Administrative Officer V)
Additional assignments
•
•
•
•
•
Member, NSRI Research and Administrative Committee (RAC)
Member, NSRI Administrative Personnel Committee (APC)
Pollution Control Officer of NSRI effective June 30, 2009. Appointment issued by
Chancellor Sergio S. Cao on July 7, 2009.
Member, UP Supervisors Association, Inc.
Proctor, UP College Admission Test (UPCAT), U.P. Diliman, Q.C., August 7-8, 2010.
Seminars/Trainings attended
•
•
•
Managing Work Place Attitude, Baguio City, May 18-21, 2010.
Resource Person. NSRI Safety Seminar, NSRI Conference Room, UP Campus,
Diliman, Q.C. January 20, 2010 and July 2010.
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
106
VILMA S. VEGA (Publication Circulation Officer II)
Additional assignments
•
•
Assistant Executive Secretary, National Committee on Marine Science (NCMS),
UNESCO Philippines (1993 – 2010).
Member, UP Supervisors Association, Inc.
Seminars attended
•
•
•
Participant/Member of the Secretariat. National Committee on Marine Sciences
(NCMS) Semi-Decadal Planning Workshop (2010-2014), Lake Island Retreat and
Seminar Center, Binangonan, Rizal. March 1-2, 2010.
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
Participant/Member of the Secretariat. National Committee on Marine Sciences
(NCMS) Outreach Marine 2010: Busuanga Island through the Marine Sciences,
Coron, Busuanga Island, Palawan. November 2-6, 2010.
ELEAZAR S. DEL REMEDIO (Administrative Officer IV)
Additional Assignment
•
Systems Administrator, U.P. NSRI, Diliman, Q.C.
Seminar attended
•
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
LELIBETH S. ALVEZA (Administrative Officer III)
Additional assignments
•
•
•
Special Collecting Officer (September 2008 – present), Approved by the Treasurer
of the Philippines, Bureau of Treasury, NCR (Appointment approved by the
Chancellor). January 1 – December 2010.
Member, UP Supervisors Association, Inc.
Member, Special Affairs Committee, UP-NSRI
Seminars attended
•
•
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
Developing Effective Work Teams by Personnel Officers Association of the
Philippines, Inc., Hotel Fleuris, Puerto Princesa City, Palawan. May 18-21, 2010.
ALVIN DC. MALLARI (Precision Instrument Technical III)
Additional assignments
•
•
NSRI-APC member, rank-and-file representative
Cluster representative, All UP-Workers Union
Study grant
•
Earned 18 units for Masters in Public Administration, NCPAG, UP Diliman, Q.C.
107
Seminars attended
•
•
•
•
Resource Person. NSRI Safety Seminar, NSRI Conference Room, UP Campus,
Diliman, Quezon City. January 20, 2010.
Resource Person. NSRI Safety Seminar, NSRI Conference Room, UP Campus,
Diliman, Quezon City. July 2010.
Permits and Licenses:
Regulatory Requirements of Chemical Substances
Importation and Usage, Diamond Hotel, Manila. November 12, 2010
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
EDEZA M. RIVERA (Administrative Assistant II)
Additional assignments
•
•
Proctor, UP College Admission Test (UPCAT), U.P. Diliman, Q.C., August 7-8, 2010.
Member, Special Affairs Committee, UP-NSRI
Seminars attended
•
•
Managing Work Place Attitude by Personnel Officers Association of the Philippines,
Inc., Hotel Veniz, Baguio City. May 18-21, 2010.
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
LILY ROSE R. DELA PAZ (Administrative Assistant III)
Additional assignment
•
Proctor, UP College Admission Test (UPCAT), U.P. Diliman, Q.C., August 7-8, 2010.
Study Grant
•
Earned 43 units for MA Guidance (Thesis stage), College of Education, U.P. Diliman,
Q.C.
Seminars attended
•
•
Permits and Licenses:
Regulatory Requirements of Chemical Substances
Importation and Usage, Diamond Hotel, Manila. November 12, 2010.
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
VICTORIANO G. BRAVO (Laboratory Technician II)
Seminar attended
•
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
EDGARDO C. GAYANES (Laboratory Aide II)
Seminar attended
•
Seminar Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
108
Photo Gallery
Fig.40. Team Building/Planning Workshop, Ilocos Sur & Norte April 6-9, 2010.
Fig.41. Seminar -Workshop on Total Quality Workplace Management (5S), NSRI Conference
Room, NSRI, UP Campus, Diliman, Q.C. September 17, 2010.
3.2 RESOURCE GENERATION AND ALLOCATION
The NSRI’s resources are derived mainly from the U.P. Budget. Additional resources are
generated from external grants to the NSRI research staff and the funds from technical
extension services. A brief description of each source and their corresponding allocation is
given below:
NSRI Budget
The NSRI’s Internal Operating Budget for 2010 is shown in Table 12.
The NSRI budget from the U.P. revolving fund is shown in Table 13. It showed how much
allocation was given to research grants by discipline, how much went to MOE, honoraria and
URA’s salaries.
Funds from Technical Extension Services
The funds from technical extension services go to the UP Trust Account. It is used mainly
to purchase minor equipment and additional supplies for the research and service
laboratories as well as photocopying units. Also, it is used to hire non-UP contractual personnel
as needed, and to provide incentives for the regular staff. The NSRI makes available through
these services the expertise of its staff and the use of its maintained scientific equipment. The
extension services fill a strong need of outside agencies, particularly medium level business
and government laboratories with inadequate facilities and for specialized services like
analytical services, microbiological services, etc. The technical extension services provide a
great opportunity for the University of the Philippines to fulfill a critical national need and at the
same time generate funds for the Institute and the University.
Table 12. The NSRI’s Internal Operating Budget in 2010.
Appropriation
Particulars
Total
Allocation
Reserve
Withheld
FOR: RESEARCH GRANTS
(Current Operating Expenditures)
I. Personnel Services
1. Permanent Positions
a. Number
b. Salary per PSI
c. Salary differences
Adjusted TOTAL
2. Lump sum for wages,
contractuals, substitute, faculty,
graduate student, assistants and
consultants
3. Lump sum for lecturers, honoraria,
allowances and hazard pay
4. PERA/ACA/Bonus/Cash Gift
5. Magna Carta
6. GSIS life & ret., Medicare, ECIP
TOTAL FOR PS
4,826,000
P 4,826,000
1,784,000
1,784,000
6,610,000
111
cont. of table 12.
II. Maintenance & Other Operating
Expenses (MOOE)
1. Regular
2. Utilities
3. Janitorial
4. UP Provident Fund HMO
TOTAL MOOE
2,702,000
2,702,000
2,702,000
GRAND TOTAL APPROPRIATIONS
9,312,000
FOR: REGULAR GRANTS
(Current Operating Expenditures)
I. Personnel Services
1. Permanent Positions
a. Number
b. Salary per PSI
Adjusted TOTAL
2. Lump sum for wages,
contractuals, substitute, faculty,
graduate student, assistants and
consultants
3. Lump sum for lecturers, honoraria,
allowances and hazard pay
4. RATA, PERA,ACA, PIB, Clothing,
Christmas Bonus, Cash Gift
Magna Carta
6. GSIS,PhilHealth,ECIP, PAGIBIG, RLIP
TOTAL FOR PS
II. Maintenance & Other Operating
Expenses (MOOE)
1. Regular
2. Utilities
3. Janitorial/Guard
4. UP Provident Fund HMO
TOTAL MOOE
GRAND TOTAL APPROPRIATIONS
6,120,000
6,120,000
234,000
234,000
116,000
116,000
1,665,000
831,000
1,665,000
831,000
8,966,000
576,000
1,292,000
430,000
576,000
1,292,000
430,000
2,298,000
P 11,264,000
112
10
3
3
1
2
28
CHEMISTRY
MATHEMATICS
ENVI. SCI. & MET.
NSRI Research
Staff
NSRI Commitment
TOTAL
234,513
100,000
455,500
200,000
1,254,974
1,048,875
AMOUNT
P 3,293,862
MOE
27
-
1
5
3
10
8
PL
4
-
0
0
1
2
1
CO-PL
0
-
-
0
0
0
0
PC
RESEARCHERS
HONORARIA
0
-
-
0
0
0
0
C
16
-
-
6
5
1
4
CO
113
P 1,121,280
-
0
163,200
182,880
394,800
380,400
Honoraria
RESEARCH GRANT FOR 2010
PL=Project Leader; CO-PL=Co-Project Leader; PC=Program Coordinator; C=Consultant; CO=Collaborator
8
No. of
PROJECTS
Granted
BIOLOGY
DISCIPLINE
Table 13. Allocation of NSRI Research Funds by Discipline in 2010.
26
4
0
4
0
10
8
URA 1
P 6,700,668
1,030,872
0
1,030,872
0
2,577,180
2,061,744
Salary
CONTRACTUAL SERVICES
P 11,115,810
1,265,385
100,000
1,649,572
382,880
4,226,954
3,491,019
TOTAL
3.3 FISCAL RESOURCES
Financial Assistance from the College of Science through the National Science
Complex was used for the following:
EQUIPMENT:
Quantity
1 unit
1 unit
1 unit
1 unit
1 unit
1 unit
1 unit
1 unit
1 unit
1 unit
1 unit
1 unit
1 lot
1 unit
1 unit
1 unit
1 lot
1 lot
1 unit
1 unit
1 lot
2 units
3 units
5 units
1 unit
1 lot
1 unit
1 unit
1 unit
1 unit
2 sets
5 units
2 units
1 unit
1 unit
1 lot
1 lot
1 set
1 unit
1 unit
1 unit
1 unit
1 unit
1 lot
2 units
1 unit
1 unit
1 unit
1 set
1 unit
1 unit
2 units
Particulars
Genetic Analyzer
Semi-Automated Microbial Identification System
Triple Quadruple Liquid Chromatograph MS/MS
Portable bench top cryostat
Graphite Furnace Analyzer
Homogenizer/ Stomacher
Hydride Vapor Generator
Microwave Digestion System
Comet Assay IV Image Analysis Software
Memmert Precision Incubator
Semi-Micro Analytical Balance
Autoclave
Office Equipment/Furniture
General Purpose Centrifuge
Top hot plate, aluminum
Ultrasonic Bath
Office Equipment/Furniture
Office Equipment/Furniture
Laboratory microscope
Digital Camera
Single channel pipettor, etc.
Refrigerator
2 HP Aircon, etc
Steel Executive Table
Airconditioner
Office Equipment/Furniture
Analytical Balance
Mupid Exu Submarine Type Electrophoresis System
Hotplate stirrer
Microwave Oven
Computer, AMD Processor
Cabinet, 6 layer with sliding glass door
Serological pipettor
Pipette Multichannel 8-channel
Benchtop pH meter
Office Equipment/Furniture
Scanner, etc
Intel Core 2 Quad
Pressure cooker
Multifunctional Laser Facsimile Machine
Universal Oven
Bench type pH meter
Stainless steel manifold
Millipore glass filter funnel/flask
Bacticinerator
Waterbath
Laboratory Mixer
Notebook
Computer, AMD Processor
Power supply
High output vacuum
Vortex Mixer
Amount
P11,042,210.00
3,676,860.00
15,250,000.00
920,000.00
1,430,000.00
239,800.00
446,500.00
1,768,233.00
240,500.00
208,150.00
111,919.00
287,000.00
570,969.00
442,198.00
P81,752.00
84,600.00
134,710.00
92,880.00
921,000.00
477,000.00
48,221.20
47,996.00
49,590.00
44,500.00
45,999.00
44,000.00
49,980.00
36,000.00
19,500.00
9,326.00
32,700.00
46,360.00
45,949.20
34,220.00
49,800.00
36,400.00
44,000.00
25,800.00
30,000.00
33,500.00
49,900.00
49,500.00
49,000.00
47,000.00
49,600.00
34,500.00
15,000.00
39,000.00
16,350.00
48,000.00
49,500.00
37,000.00
114
1 unit
1 unit
2 units
2 units
1 lot
1 lot
1 unit
1 unit
1 unit
1 unit
1 lot
1 unit
3 sets
1 unit
1 unit
1 unit
1 unit
1 unit
3 sets
1 unit
1 lot
1 unit
1 unit
3 units
2 units
1 unit
1 unit
Mini-sub cell GT system
Sterilizer
Upright chiller
Microwave oven
Millipore Funnel holder
Outdoor camera, etc.
Digital Camera
Paper Shredder
Digital Microcentrifuge
Digital Dry Bath
Laminating Machine, etc.
Grass cutter
Computer
DLP Projector
Digital copier
Digital dry bath
Pipette Aide
PVC Dessicator vertical cabinet
Computer
Ultrasonic cleaner
D-link, 24 port, scanner, etc.
Portable centrifuge
Vortex mixer
Micropipet, adjustable (3's)
Compact balance
Power supply
High output vacuum
34,000.00
30,000.00
39,300.00
9,100.00
46,605.00
49,988.80
48,200.00
17,950.00
47,700.00
46,612.00
49,325.00
14,760.00
49,500.00
49,975.00
42,500.00
33,500.00
16,500.00
44,000.00
49,500.00
46,658.00
45,990.00
43,597.00
49,500.00
40,800.00
39,800.00
48,000.00
49,500.00
P 40,669,833.20
INFRASTRUCTURE:
A. Construction Management of Service for the
Rehabilitation and Renovation of NSRI Bldg.
(Renovation of comfort rooms, retilling of floors,
installation of vinyl partition, replacement of water
closet, urinals, lavatory sink, faucet, water supply
exhaust fan and repainting; construction of chemical
waste storage; improvement of NSRI rooftop drying
area; construction of roof for the path walk between
RASL and Pesticide Lab and tiling of all NSRI hallways)
B. Laboratory Animal Facility for BRSL
C. Refurbishment of DNA Analysis Laboratory
D. Conversion of Chemical Waste Storage Facility into the
Fermentation Laboratory of MRSL
E. NSC Site Development – Package 4
F. NSRI parking area, perimeter fence, etc.
TOTAL FOR EQUIPMENT & INFRASTRUCTURE
11,376,256.65
8,249,990.73
19,626,157.53
P 60,295,990.73
Vvvvvvvvvvvv
115
˜ Future Plans and Recommendations ™
Purchase of new equipment and renovation of facilities courtesy of the funds from the
National Science Complex were done in 2010. These will allow for the Institute of continue and
embark on new research activities. At the same time, the technical extension services of the
in-house laboratories are being planned to be expanded to cater to more analyses that are
not easily available outside, even in other academic and research institutions. What makes
these services different from other provider are the research and development activities that
are undertaken to ensure that procedures are validated and that results are reliable and
acceptable even internationally.
Much of the challenge will lie on the maintenance of these equipment and facilities.
Strategies on how to find the funds to maintain them and keep the activities running should be
resorted to. The judicious use of facilities and resources will also be implemented.
116
Ernelea P. Cao, Ph.D.
Executive Editor
Ms. Vilma S. Vega
Managing Editor
Mr. Florentino P. Abundo
Natural Sciences Research Institute
College of Science
Miranda Hall, corner Quirino and Velasquez Sts., Diliman, Quezon City