Biotechnology
CTY Course Syllabus
Day 1, Monday
Morning
Topic/Objective
What time? Method?
Introduction
− Expectations/Syllabus/Honor Code/Grading/Final
Project (Pay it Forward) (20 minutes)
− Ice Breaker (20 minutes)
Pre-Assessment
Introductory Topics
Ω What is Biotechnology?
Ω History of
Biotechnology
Ω The Scientific Method
Ω Prokaryotes vs.
Eukaryotes
Cell Theory (structure vs.
function)
The History of DNA
DNA Principles
Review
Afternoon
− Test (30-40 minutes)
− PowerPoint Lecture (40 minutes)
− Exercise: Cell City Activity (30 minutes)
− Exercise: Who did what? (30 minutes)
− Exercise: Building DNA Models (30 minutes)
− Key principles of the day (15 minutes)
Topic/Objective
What time? Method?
Laboratory Safety
− Safety Video?
− Safety Contract
Basic Metric System
− Conversion Exercises (20 minutes)
Common Laboratory
Equipment
Laboratory Notebook
Procedures
Laboratory Techniques
− Tour of the Laboratory, PPE
− Standard Guidelines Sheet with Examples
− Lab Exercises #1a: Micropipette Techniques
(bb_micropipettetech_09) (30 minutes)
− Lab Exercises #1b: Gel Electrophoresis of Dyes
(bb_gelelectrophoresisdyes_09) (45 minutes)
Evening
Topic/Objective
What time? Method?
Micropipetting Exercise and Gel
Electrophoresis
− Lab Write up in Notebook (30 minutes)
Reading Assignment
− Chapter 2 Pgs. TBD
“Pay it Forward” Project
Day 2, Tuesday
− Decide on research topic
Morning
Topic/Objective
What time? Method?
Question/Answer
− Students can ask questions on any of the key
points covered
DNA Replication
− PowerPoint lecture (40 minutes)
Key Points from Day 1
DNA → RNA → Protein
Ω Transcription
Ω Translation
Ω RNA Processing
Gene Expression/Regulation
Ω Prokaryotes vs.
Eukaryotes
Review
Afternoon
− Quiz #1 (Multiple Choice) (20 minutes)
− Exercise: Enzyme Roulette? (Matching
Game/Team Activity) (40 minutes)
− Exercise: Am I on or off? (Group Activity) (30
minutes)
− Key principles of the day (30 minutes)
Topic/Objective
What time? Method?
DNA Extraction from Food
− Pre-laboratory Exercises (30 minutes)
− Laboratory #2: DNALC Strawberry DNA Extraction
(40 minutes)
Evening
Topic/Objective
What time? Method?
DNA Extraction from Cheek
Swabs
− Laboratory #3: Genes in a Tube (1 hour)
Reading Assignment
− Background on Mitosis and Meiosis (provided by
instructor)
Topic/Objective
What time? Method?
Assignment
− Mitosis/Meiosis Question/Answer Worksheet
Day 3, Wednesday
Morning
Topic/Objective
What time? Method?
Q & A session
− Students questions about key material
“Pay it Forward” Research
Project
− Submit topic in writing for approval
Key Points from Day 2
Chromosomes
Introduction to Mitosis Cell
Cycle (Cancer)
− Quiz #2 (Multiple Choice) (20 minutes)
− PowerPoint Lecture (40 minutes)
− Exercise: Karyotype Diagram (Group Exercise)
Afternoon
Topic/Objective
What time? Method?
Meiosis
− PowerPoint Lecture and Diagrams
Inheritance Traits
Ω Gregor Mendel
− Lecture/Diagrams
Ω Example Problems
Ω Handout Genetics Problem Worksheet
Differences between Mitosis and
Meiosis
Sickle Cell Laboratory
− Review the Mitosis/Meiosis Handout given for
Study Hall
− Pre-Laboratory Exercises
Evening
Topic/Objective
What time? Method?
“Pay it Forward” Research
− Computer Lab: Start finding information on topic
Reading Assignment
− Chapter 3 Pgs. TBD
Genetics
− Complete Genetics Problems Worksheet and submit
it to the TA
Day 4, Thursday
Morning
Topic/Objective
What time? Method?
Question/Answer
− Student questions about key material
Restriction Enzymes
− PowerPoint/Lecture
Key Points from Days 1-3
Afternoon
− Test #1: Multiple Choice/Short Answer/Essay (40
minutes)
Topic/Objective
What time? Method?
How Southern Blots Work?
(Review)
− Lecture/PowerPoint
How PCR works? (Review)
PCR Amplification Lab
− Pre-Laboratory Exercises (30 minutes)
− Laboratory #5: Start PCR Amplification of DNA
Evening
Topic/Objective
What time? Method?
Continue PCR Amplification
Lab
− Laboratory #5: Finish the PCR Lab and Complete
Write-Up
Reading Assignment
Day 5, Friday
− Chapter 4 Pgs. 100-121
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student questions about key material
Introduction to Proteins
Ω Protein Structure:
Primary, Secondary,
Tertiary, and
Quaternary.
Ω Methods of Purification
− PowerPoint Lecture
Key points from Day 4
Review
− Quiz #3: Multiple Choice (20 minutes)
− Key Principles of the Day
Afternoon
Topic/Objective
What time? Method?
Enzyme Review
− PowerPoint Lecture
Protein Laboratory
− Pre-Laboratory Exercises
− Laboratory #6: Carolina Cheese Production
BioKit
Evening
Topic/Objective
What time? Method?
Protein Laboratory
− Laboratory #6: Finish the Cheese Lab and
Complete Write-Up
Handout Research Papers
about GFP
− Read paper and be prepared to summarize how GFP
was used in the different model systems
Reading Assignment
Day 6, Monday
− Chapter 5: Pgs. 122-157
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student questions about key material
Microbial Biotechnology
Bacterial Transformation
Vaccine Research
− PowerPoint Lecture
Key Points from Day 5
The Joy of GFP
Review
Afternoon
− Quiz #4: Multiple Choice (20 minutes)
− Exercise: GFP: the amazing gene
− Key Principles of the Day
Topic/Objective
What time? Method?
Transformation Procedures
− PowerPoint Lecture
Review how transformation
works
− Pre-Laboratory Exercises
− Laboratory #7: E. Coli with Blue and Green
Fluorescent Proteins
Evening
Topic/Objective
What time? Method?
“Pay it Forward” Research
Project
− Work on project
− Directed Reading: Chapter 6, 7 Pgs. TBD
Day 7, Tuesday
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student Questions about key material
Key Points from Day 4, 5, 6
Afternoon
− Test #2: Multiple Choice/Short Answer/Essay
Topic/Objective
What time? Method?
Transformation Procedures
− Laboratory #7: E. coli transformation lab finish
experiment and complete write-up
Evening
Topic/Objective
What time? Method?
Free study period
− Work on Debate Arguments (Group Activity)
Reading Assignment
Day 8, Wednesday
− Crime to Courtroom Handouts (provided by
instructor)
− Chapter 8 pgs. TBD
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student Questions about key material
Crime Scene Investigation
Ω Chain of Evidence
Ω Collection and Types of
Evidence
− Exercise: Mock Crime Scene
Ω Role-play Experience (provided by
instructor)
Topic/Objective
What time? Method?
The Scientific Method and
Forensic Science
− PowerPoint Lecture
− Exercise: ACE-V Fingerprinting Identification
Method and Questioned Document Analysis
Review
Afternoon
Topic/Objective
What time? Method?
Biotechnology and Forensic
Science
Ω STRs
Ω DNA Fingerprinting
Ω mtDNA Analysis
− PowerPoint Lecture
− Exercise: RFLP Simulation Activity
DNA Fingerprinting Laboratory
− Pre-Laboratory Exercises
− Laboratory #8: DNA Fingerprinting Using
Restriction Enzymes
Evening
Topic/Objective
What time? Method?
Free Study Period
− Work on Debate Arguments
− ”Pay it Forward” Research Projects
Reading Assignment
Day 9, Thursday
− Chapter 9, 10 Pgs. TBD
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student Questions about Key Material
Key Points from Day 7, 8
Bioremediation
Ω Chemical
Contamination
Ω Turning Waste into
Energy
Case Studies
Review
− Quiz #5: Multiple Choice (20 minutes)
− Discussion: Environmental Accidents and the
Solutions
Afternoon
Topic/Objective
What time? Method?
DNA Fingerprinting Laboratory
− Laboratory #8: Complete the DNA Fingerprinting
Lab and Complete the write-up
Evening
Topic/Objective
What time? Method?
Free Study Period
− Work on Debate Arguments
− ”Pay it Forward” Research Projects
Reading Assignment
Day 10, Friday
− Directed Reading: Chapter 11, 12 Pgs. TBD
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student Questions about Key Material
Medical Biotechnology
Ω Sickle Cell
Ω Cancer
Ω Stem Cells
− PowerPoint Lecture
− Exercise: Blood Typing
Key Points from Days 7, 8, 9
Detection Methods
Ω Antibodies
Ω ELISA
− Quiz #6: Multiple Choice/Short Answer
− Exercise: Pedigree Analysis
Afternoon
Topic/Objective
What time? Method?
The Mechanism Behind
Retroviruses
− Lecture
ELISA Review
ELISA Laboratory
− PowerPoint
− Laboratory #9: AIDS Kit I: Simulation of HIV
Detection by ELISA
Evening
Topic/Objective
What time? Method?
Free Study Period
− Work on Debate Argument
− ”Pay it Forward” Research Projects
− Finish any previous Lab write-ups
Reading Assignment
Day 11, Monday
− Directed Reading Chapter 13 TBD
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student Questions about Key Material
Key Points from Day 10
Biotechnology Regulations
Review
GMO Foods
− Quiz #7: Multiple Choice/Short Answer (20
minutes)
− Laboratory #10: Identifying GMO Foods Using
PCR
Afternoon
Topic/Objective
What time? Method?
GMO Foods
− Laboratory #10: Identifying GMO Foods Using
PCR
Evening
Topic/Objective
What time? Method?
Free Study Period
− Finish “Pay it Forward” Research Project
Day 12, Tuesday
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student Questions about key material
“Pay It Forward” Research
Paper
Ethics and Biotechnology
Review for the Final Test
Afternoon
− Due at the beginning of class
− Handout Review sheets and review all the key
information (Group Activity Game)
Topic/Objective
What time? Method?
GMO Foods Lab
− Laboratory #10: Complete the GMO Foods
Laboratory and Write-Up
Evening
Topic/Objective
What time? Method?
Free Study Period
− Finish all lab reports/Review all material
Day 13, Wednesday
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student Questions about key material
Laboratory Notebook
− Lab Notebooks Due before Afternoon Class
Inclusive
Afternoon
− Final Test #3: Multiple Choice/Short Answer/Essay
Topic/Objective
What time? Method?
Video
− GATTACA
Topic/Objective
What time? Method?
Free Study Period
− Finalize debate arguments (Monsanto)
Evening
Day 14, Thursday
Morning
Topic/Objective
What time? Method?
Question/Answer Session
− Student questions about key material
Topic/Objective
What time? Method?
Posters (“Pay it Forward”)
− Complete posters for display
Evening
Day 15, Friday
Morning
Topic/Objective
What time? Method?
Poster Presentations
− Display posters and answer any questions