Towards non-human primate models for autism spectrum disorders --Autism-like behaviors of MeCP2 transgenic monkeys Zilong Qiu Methyl-CpG binding protein 2 (MeCP2) plays critical roles in transcriptional regulation and microRNA processing. Mutations in MECP2 gene are found in 90% of patients with Rett syndrome, a severe form of developmental disorders with autistic phenotypes. Duplications of MECP2–containing genomic segments cause the MECP2 duplication syndrome, which shares core symptoms with autism spectrum disorders (ASD). Although the Mecp2-null mice recapitulate most developmental and behavioral defects found in Rett syndrome patients, it has been difficult to identify autism-like behaviors in the mouse model with MeCP2 overexpression. Here we report that lentivirus-based transgenic cynomolgus monkey (Macaca fascicularis) expressing human MeCP2 in the brain exhibited autism-like behaviors and showed germline transmission of the transgene. Expression of the MECP2 transgene was confirmed by Western blotting and immunostaining of brain tissues of transgenic monkeys. Genomic integration sites of the transgenes were characterized by deep-sequencing. As compared to wild-type (WT) monkeys, MECP2 transgenic (TG) monkeys exhibited higher frequency of repetitive circular locomotion and elevated stress responses, as measured by the threat-related anxiety and defensive (TAD) test. The TG monkeys showed less interaction with WT monkeys within the same group (reared together for ~ 6 months) and also reduced interaction time when paired with another TG monkey in social interaction tests. The cognitive functions of TG monkeys were largely normal in Wisconsin General Test Apparatus, although some showed signs of stereotypic cognitive behaviors. Notably, we succeeded in generating 5 F1 offspring of MECP2 TG monkeys by intracytoplasmic sperm injection with sperms from one TG monkey, showing germline transmission and Mendelian segregation of multiple MECP2 transgenes in the F1 progeny. Together, these results indicate the feasibility and reliability of using genetically engineered non-human primates for studying brain disorders.