This article was downloaded by: [University of Minnesota Libraries, Twin Cities] On: 23 October 2012, At: 15:40 Publisher: Routledge Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK Communication Quarterly Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/rcqu20 Nuances in inoculation: The role of inoculation approach, ego‐involvement, and message processing disposition in resistance a b Michael Pfau , Kyle James Tusing, Waipeng Lee , Linda C. Godbold, Ascan Koerner, Linda J. Penaloza, Yah‐huei Hong & Violet Shu‐huei Yang a Professor in the School of Journalism and Mass Communication, University of Wisconsin, Madison b Lecturer in the School of Communication Studies, Nanyang Technological University, Singapore Version of record first published: 21 May 2009. To cite this article: Michael Pfau, Kyle James Tusing, Waipeng Lee, Linda C. Godbold, Ascan Koerner, Linda J. Penaloza, Yah‐huei Hong & Violet Shu‐huei Yang (1997): Nuances in inoculation: The role of inoculation approach, ego‐involvement, and message processing disposition in resistance, Communication Quarterly, 45:4, 461-481 To link to this article: http://dx.doi.org/10.1080/01463379709370077 PLEASE SCROLL DOWN FOR ARTICLE Full terms and conditions of use: http://www.tandfonline.com/page/terms-andconditions This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 arising directly or indirectly in connection with or arising out of the use of this material. Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 Nuances in Inoculation: The Role of Inoculation Approach, Ego-Involvement, and Message Processing Disposition in Resistance Michael Pfau, Kyle James Tusing, Waipeng Lee, Linda C. Godbold, Ascan Koerner, Linda J. Penaloza, Yah-huei Hong, and Violet Shu-huei Yang THIS investigation attempted to provide further understanding of the inoculation model of resistance to influence. It examined the efficacy of inoculation treatments designed to promote central versus peripheral message processing in instilling resistance to influence, taking into account message processing disposition. The study also tested the potential of social judgment theory to explain the cognitive process which occurs in inoculation. A total of 790 participants took part in the investigation. The study employed three issues that represented low, moderate, and high involvement, and featured multiple inoculation and attack messages. The results indicate that inoculation treatments, using central and peripheral approaches, confer resistance to influence and, thus, imply that threat is more prominent than refutational preemption in the process of resistance. Further, the pattern of results suggest that greater receiver need for cognition enhances resistance, but only with highly involving issues. Finally, the results of the investigation rule out assimilation and contrast as a potential explanation of the cognitive process triggered via threat, but suggest that receiver ego-involvement facilitates threat. KEY CONCEPTS inoculation, resistance, ego-involvement, message elaboration, ELM, influence Michael Pfau (Ph.D., University of Arizona, 1987) is a Professor in the School of Journalism and Mass Communication, University of Wisconsin—Madison. Waipeng Lee (Ph.D., University of Wisconsin, 1997)is a Lecturer in the School of Communication Studies, Nanyang Technological University, Singapore. At the time this study was conducted, Linda J. Penaloza, Yah-huei Hong, and Violet Shu-huei Yang were graduate students in Journalism and Mass Communication, whereas Kyle James Tusing, Linda C. Godbold, and Ascan F. Koerner were graduate students in Communication Arts, at the University of Wisconsin—Madison. The authors thank faculty in the School of Journalism and Mass Communication, Department of Communication Arts, and the School of Business at the University of Wisconsin—Madison for assistance in recruiting subjects for this study. Communication Quarterly, Vol. 45, No 4, Fall 1997, Pages 461-481 Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 I n recent years there has been a resurgence of interest in the inoculation approach of resistance to attitude change. This renewed interest has been primarily pragmatic in emphasis, with scholars and practitioners exploring applications of inoculation in commercial, political, and adolescent health contexts. Yet, there has been little theoretical work on the core inoculation construct (Eagly & Chaiken, 1993). This investigation sought to refine and extend the core assumptions of inoculation, thereby providing greater .nuance in inoculation's predictions in future settings. Specifically, this investigation focussed on the process of inoculation. Research clearly indicates that inoculation confers resistance to subsequent influence, but exactly how? Threat and refutational preemption are central concepts in the inoculation process (McGuire & Papageorgis, 1962; Pfau, 1997), which raises a number of key questions. What is the relationship between ego-involvement and the core concepts of threat and refutational preemption? What cognitive processes are triggered via threat, and what implications do they pose for the type of inoculation message that is most effective with people who differ in message processing disposition? These and other issues remain unresolved. Inoculation Approach and Receiver Processing Disposition Much more needs to be learned concerning the refutational preemption component of inoculation treatments, particularly as it relates to receiver processing disposition. Little is known about the relative efficacy of distinctive inoculation treatment approaches, except that refutational same and different messages are comparable in their effectiveness (McGuire, 1961,1962,1966; McGuire & Papageorgis, 1961; Pfau, 1992; Pfau & Burgoon, 1988; Pfau, Kenski, Nitz, & Sorenson, 1990; Pfau, Tusing, Koerner, Lee, Godbold, Penaloza, Yang, & Hong, 1997). Beyond comparisons of same and different treatments, which were important in the early evolution of inoculation theory since they provided implicit documentation of the integral role threat plays in the process of resistance (Pfau, 1997), there has been very little research concerning inoculation treatment approach, and none exploring treatment approach in conjunction with message processing disposition. The involvement and message processing literature offers a rationale for examining treatment approach in conjunction with receiver processing proclivity. The theory and research here identifies two routes to influence: one that is more thoughtful, featuring active elaboration of message content; and the other less thoughtful, with an emphasis on simple inferences derived from cues attached to the messages, such as affect and source. When placed in theoretical context, the two routes are identified as "central" and "peripheral" in the ELM (see Petty & Cacioppo, 1986). Inoculation is assumed to be an active process. The threat component of an inoculation treatment motivates the receiver to engage in bolstering of attitudes against potential challenges, whereas the refutational preemption component identifies specific counterarguments and then provides the reasoning and evidence to refute them. The process of inoculation, which Eagly and Chaiken (1993, p. 564) characterize in terms of "covert counterarguing," is thus assumed to be active (McGuire, 1964). Inoculation works by motivating receivers to engage in issue-relevant thought, both concerning the specific arguments that are noted and refuted in the inoculation treatment as well as additional arguments that a receiver identifies on his/her own. 462 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 Inoculation presupposes careful elaboration of the arguments and evidence contained in a treatment message. One recent study found that, absent adequate message elaboration, in this case as a result of distraction following administration of treatments, inoculation was precluded (Chen, Reardon, Rea, & Moore, 1991). We argue that the active process of inoculation corresponds to the type of mindful message processing identified in the ELM as "central." Inoculation treatments by their very nature confront the individual with challenges to attitudes, providing both the motivation and specific content to bolster attitudes. They resemble "incongruent messages," which Maheswaran and Chaiken (1991) found are more likely to result in systematic information processing. Furthermore, once threat is triggered, messages which contain peripheral cues may undermine issue-relevant thinking, which parallels research findings for high involvement conditions (Petty & Cacioppo, 1986; Petty, Cacioppo, & Heesacker, 1981). Thus, the efficacy of inoculation treatments should be enhanced via treatment messages that place greater emphasis on reasoning and evidence, which are targeted to receivers disposed to more active message processing. HI: Inoculation treatments which are constructed so as to promote central message processing are superior to those constructed to promote peripheral processing in conferring resistance to persuasive attacks for those people who receive an inoculative treatment as opposed to those who do not What determines whether receivers engage in more active or mindful message processing? An individual's need for cognition may be the best predictor of message processing tendencies. An individual higher in need for cognition expends more cognitive effort evaluating message content (Cacioppo, Petty, Feinstein, & Jarvis, 1996), which facilitates both comprehension (Cacioppo et al., 1996) and influence of message content (Cacioppo, Petty, & Morris, 1983). Conversely, receivers lower in need for cognition are more likely to turn to "extrinsic persuasive cues" (Chaiken, 1987) in evaluating messages. Thus, this study predicts that H2: Inoculation confers more resistance to persuasive attacks among those people who receive an inoculative treatment who are higher in need for cognition. The Role of Ego-Involvement McGuire grounded inoculation on axioms of persuasion that were prevalent during the 1950s (e.g., selective exposure), but was somewhat tentative concerning the internal processes that inoculation unleashes. McGuire identified the concepts of threat and refutational preemption as core axioms in inoculation theory. Threat, which is defined as receiver acknowledgment of potential susceptibility of attitudes to subsequent influence, functions as the motivational catalyst to resistance, whereas refutational preemption provides specific content for receivers to employ in order to strengthen attitudes against change. What cognitive process does threat trigger in receivers that fosters resistance to same and different counterarguments? One plausible explanation is assimilation and contrast, which have their origins in social judgment theory (Sherif & Hovland, 1961; Nuances in Inoculation 463 Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 Shérif & Shérif, 1967; Shérif, Shérif, & Nebergall, 1965). The theory conceptualizes attitude based on an evaluative continuum divided into three parts or latitudes: acceptance, rejection, and noncommitment The latitude of acceptance includes positions a receiver finds acceptable in comparison with their own view, whereas latitude of rejection encompasses positions a receiver finds unacceptable. The latitude of noncommitment features those positions a person finds neither acceptable nor unacceptable. In social judgment theory, persuasion is viewed as a two-stage judgmental process (Smith, 1982). Upon exposure to a persuasive message, the receiver views the message in terms of their initial attitudinal position, which serves as an "anchor." Initially, if the message falls within the range of acceptance or rejection, there is a tendency to distort the message's content Respondents tend to minimize a discrepancy between the anchor and a message when the latter falls in the range of acceptance, but maximize a discrepancy when the message falls in the range of rejection. Subsequently, attitude change is a function of the discrepancy between the message and the anchor, resulting in the movement of the anchor toward the message in the event that a message falls within the ranges of acceptance or noncommitment (assimilation), or in the movement of the anchor away from the message in the event that it falls in the range of rejection (contrast). Assimilation facilitates influence, whereas contrast inhibits influence, and may produce a boomerang effect (Kiesler, Collins, & Miller, 1969). The division of the evaluative continuum depends entirely on receiver egoinvolvement in the content area. Sherif and Cantril define ego-involvement as receiver identification with an attitude. An ego-involved attitude is internalized as part of a person's value system. It is perceived as part of one's self: "as being part of me" (Sherif & Cantril, 1947, p. 93). An ego-involved attitude is "...inextricably linked to other aspects of the self...to important group memberships and identifications" (Eagly & Chaiken, 1993, p. 369). Greater ego-involvement in an issue reduces the range of noncommitment, extends the range of rejection, and thus enhances the prospect that a message will invoke the process of contrast (Sherif, Taub, & Hovland, 1958). Inoculation treatments, because they contain content which is consistent with existing attitudes, should locate within the receiver's range of acceptance. Thus, an inoculation treatment initially should trigger assimilation, in which the receiver's attitude shifts even further toward the position advocated in the message. The refutational preemption component of the inoculation message would be responsible for this assimilation effect In addition, the threat component of an inoculation message provides an additional contribution. The threatening material should magnify receiver egoinvolvement, which generates sizable discomfort in receivers (Eagly & Chaiken, 1993). Higher levels of ego-involvement should extend the range of rejection, which contains beliefs viewed as unacceptable (Sherif & Sherif, 1967). In this manner, inoculation treatments may simultaneously strengthen existing attitudes and lay a foundation for subsequent resistance. The effect of the initial shift in attitudes which is produced by the treatment, and to an even greater degree, the extension of the latitude of rejection caused by the threatening material, combine to confer resistance to influence. Thus, when a person subsequently encounters an attack message, it triggers an even more pronounced contrast effect, and therefore greater distortion of the attack message, and thus more resistance to it 464 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 The assimilation and contrast explanation for the process of inoculation has received limited attention to date. The only empirical test of assimilation and contrast in inoculation was provided fay Manís and Blake (1963), who found that subjects who received passive immunizations were more resistant to influence. They also found that immunized subjects distorted the content of subsequent attacks in the direction of the inoculation treatment To test for assimilation and contrast, this study examined attitudes at two points in time: immediately after administration of inoculation treatments, and once again, following exposure to the attack materials. This study posits that, immediately after administration of inoculation treatments: H3: H4: Inoculation immediately strengthens the existing attitudes of those people who receive an inoculative treatment as compared to those who do not. The proclivity of inoculation to immediately strengthen existing attitudes is the most pronounced among more egoinvolved receivers. However, following exposure to the attack materials, the study predicts that H5: Inoculation subsequently confers resistance to persuasive attacks among those people who receive an inoculative treatment as compared to those who do not The concept of ego-involvement is not restricted to use in social judgment theory. Ego-involvement is defined in a broader sense as personal relevance of a content area to an individual (Greenwald, 1982). Personal relevance determines the energy an individual expends in message processing. As Petty and Cacioppo (1986, p. 149) observe: "Personal relevance enhances motivation to process issue-relevant arguments." As a result, more receiver ego-involvement should facilitate the efficacy of inoculation treatments in conferring resistance to subsequent attacks, independent of the explanatory mechanisms of assimilation and contrast The more ego-involved in a content area, the more likely that an individual will be able to access relevant attitudes (Fazio, 1989). This should facilitate threat, which requires that receivers perceive potential vulnerability of attitudes. It is the threatening component of inoculation which motivates the individual to expend the cognitive energy required to bolster attitudes (Pfau, 1997). This rationale is consistent with the notion that cognition requires effort, and since people tend to act economically, they are unlikely to expend cognitive energy attempting to strengthen unimportant attitudes. This is the same reasoning that underlies the ELM and the HSM, both of which have received considerable support in extant research (e.g., Chaiken, 1980; Chaiken, Liberman, & Eagly, 1989; Johnson & Eagly, 1989; Petty & Cacioppo, 1979, 1984,1986, 1990; Petty, Cacioppo, & Goldman, 1981). This study predicts: H6: H7: The tendency of inoculation to generate threat is most pronounced among more ego-involved receivers. The tendency of inoculation to confer resistance to persuasive Nuances in Inoculation 465 Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 attacks is most pronounced among more ego-involved receivers. METHOD Topic Selection This investigation required three issues, representing low, moderate, and high issue involvement, and reflecting a roughly equal distribution of opinion both for and against Use of the concept issue involvement, in contrast to ego-involvement, was limited to the topic selection phase of the study. For purposes of topic selection, issue involvement was operationalized as the importance or salience of the issue to the receiver. Researchers brainstormed possible topics, preparing policy propositions on 31 topics. After lengthy discussion, researchers settled on 16 policy propositions that seemed to meet criteria described above. The 16 propositions were included in a survey instrument designed to measure issue involvement and distribution of opinion. A total of three 10-interval scales were employed to assess a person's issue involvement in each topic, adapted from an instrument originally developed by Traylor (1981) to gauge product involvement. The scale items included: importance of the issue, interest in the issue, and relevance of the issue. The distribution of opinion was assessed using a single item: agree, disagree, or neutral/no opinion. The survey was administered to 118 university undergraduate students (the same population that was scheduled to be the focus of the subsequent study). Results of the survey revealed three topics that meet the criteria, representing low, moderate, and high issue involvement, and reflecting roughly equal distribution of opinion both for and against the proposition. Final topics selected for the study included: whether manufacture, sale, and possession of handguns should be banned throughout the nation (involvement mean: 7.52 on a 1-10-point issue involvement scale); whether the U. S. should legalize the sale and use of marijuana (involvement mean: 5.95); and if gambling should be completely legalized throughout the U. S. (involvement mean: 4.77). Participants Participants were recruited from introductory courses in three departments at a midwestern university. The study featured three distinct phases extending over a period of three weeks. A total of 790 subjects completed all three phases (a retention rate of 82.7%). Design, Independent Variables, and Covariates The investigation employed a 3 X 2 factorial design. The independent variables included experimental condition and need for cognition. Experimental condition was operationalized as cognitive inoculation treatment, peripheral treatment, and no inoculation (control). Effectiveness of inoculative treatments in deflecting the influence of persuasive attacks was gauged by comparing receiver attitudes following exposure to the attack, and by examining the amount of attitude change induced by the attack. Receiver need for cognition was operationalized according to responses on Cacioppo, Petty, and Kao's (1984) abbreviated need for cognition scale (NCS). NCS is designed to "distinguish" those people "who dispositionally tend to engage in and 466 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 enjoy effortful analytic activity" from others (Cacioppo et al., 1983, p. 806). The shortened 18-item version of the NCS has been found to relate very positively with its more lengthy 34-item parent (Cacioppo et al., 1984). Receiver need for cognition scores were subsequently dichotomized based on a median split. Receiver ego-involvement was treated as a covariate. Ego-involvement was operationalized in terms of identification with partisan interest groups which advocate a position consistent with a receiver's attitude. Sherif and colleagues operationalized ego-involvement in their research as "subjects memberships in or identification with groups that were known to actively support particular positions on...issues" (Eagly & Chaiken, 1993, p. 369). This study employed a method of determining ego-involvement previously used by Perloff (1989). Receiver group identification was evaluated by asking: on a scale of 0 to 100, where 0 denotes no identification and 100 indicates very strong identification, to what extent a person identifies with partisan organizations who advocate positions consistent with their attitudes. Procedures Researchers prepared a number of messages for administration during the study. Two attack messages were written for each of the three topics: one opposing the thesis of the proposition, to be administered to subjects who favored the proposition; and one favoring the thesis of the proposition, to be administered those who opposed it Each attack message was developed employing two distinct lines of argument, with each argument supported using an equal portion of substantive (general statistics and studies) and peripheral (source credibility and anecdotes) backing. The six attack messages ranged in length from 401 to 406 words. Four inoculation treatment messages were written in response to each attack message: two central inoculation messages and two peripheral inoculation messages. The central inoculation messages contained highly cognitive content, featuring arguments supported by statistics and studies. The peripheral inoculation treatments employed a combination of affect and source appeals, featuring arguments supported by anecdotes and source expertise. The first paragraph of each inoculation message contained the threat component Threat was operationalized as a warning of impending potentially persuasive attacks against the position on the topic supported by the receiver. The rest of the inoculation message featured answers to arguments that could be raised in a potential attack, with central messages using highly cognitive content and peripheral messages using a combination of affect and source appeals. The 24 inoculation messages ranged in length from 249 to 256 words. An example featuring one of the attack messages and its four corresponding inoculation messages are included in the appendix. Each inoculation message attempted to replicate the written style of its corresponding attack message. The four inoculation messages and corresponding attack message were constructed to closely match in writing style and comprehensibility. Special attention was paid to message length, average sentence length, verb tenses, modifiers, and argument style. Each message was rated for overall compressibility using Becker, Bavelas, and Braden's (1961) Index of Contingency. The ratings for the 24 inoculation and six attack messages ranged from 13.8 to 14.8, suggesting equivalence. The study was conducted in three phases from November 7 to December 3. During Nuances in Inoculation 467 Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 the first phase, all participants completed a questionnaire designed to provide demographic information and to assess issue involvement, ego-involvement, and attitudes about each of the three propositions. Participants were then assigned to specific conditions based on attitude and issue involvement Participants were told they were participating in a study about message processing. Phase 1 was administered over two days. Issue involvement was operationalized as the importance or salience of the topic. It was measured using slight modification of the Personal Involvement Inventory, which is a unidimensional scale, developed and then tested for validity and reliability by Zaichkowsky (1985). The PH examines "perceived relevance based on personal needs and interests" (Rubin, 1994, p. 286), and research indicates that it is a valid (Goldsmith & Emmert, 1991; Zaichkowsky, 1985) and reliable (Celuch & Evans, 1989; Mobley, Bearden, & Teel, 1988) measure of issue involvement The Pu items that concern desirability, and are thus more appropriate for product involvement, were eliminated in this study, and scale intervals were expanded from seven to nine. Scale items included: boring/interesting, unimportant/important, doesn't/does matter to me, nonessential/essential, of no concern/of much concern, means nothing/ means a lot, exciting/unexciting, irrelevant/relevant, superfluous/vital, trivial/fundamental, mundane/fascinating, and significant/insignificant The modified PH involvement scale achieved high reliabilities: on handguns, .94; on marijuana, .94; and on gambling, .95. Following Phase 1, researchers entered the preliminary data, analyzed issue involvement and attitude data, and then on the basis of the results, assigned participants to conditions. Issue involvement was trichotomized on each topic, and subjects scores examined. Where a person ranked the same (low, moderate, or high involvement) on two of the topics on which their overall attitude scores were either less than 4.5 or greater than 5.5 (on a 1-9-point attitude scale), therefore suggesting the presence of an attitude for or against a topic, they were assigned to those topics. Otherwise, participants were assigned randomly to one of the topics on which they displayed an attitude. Once assigned to one or two topics, the participants were randomly assigned to inoculation same, inoculation different, or control condition. Phase 2 and 3 experimental booklets were prepared for each participant The Phase 2 booklets contained inoculation message(s) supporting ata'tudinal positions for those participants assigned to same or different treatment conditions, as well as questionnaires designed to assess threat, attitudes, counterarguments, and answers to counterarguments on each topic. Phase 3 booklets contained one or two attack messages opposing attitudinal positions and questionnaires designed to evaluate participant response to the attack(s). The second phase included the administration of inoculation materials for treated participants and additional measurement, and was conducted during November 10 to 22. The third phase of the study featured the administration of attack messages to all participants and further measurement, and was conducted from November 23 to December 3. Dependent Measures Three dependent measures were employed in the investigation. Attitude was assessed with six bipolar adjective pairs previously used by Burgoon, Cohen, Miller, 468 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 and Montgomery (1978), Miller and Burgoon (1979), Pfau and Burgoon (1988), and Pfau, Van Bockern, and Kang (1992). Adjective pairs were: foolish/wise, wrong/right, unfavorable/favorable, bad/good, unacceptable/acceptable, and negative/positive. The coefficient alpha reliabilities for the attitude scale were good: handguns, .96; marijuana, .94; and gambling, .94. The threat of inoculation treatments was assessed using five bipolar adjective pairs employed previously by Pfau and Burgoon (1988), Pfau et al. (1990), Pfau (1992), and Pfau et al. (1992). Threat was measured during Phase 2. Inoculated and control participants were asked their reaction to the prospect that they may come in contact with information which may cause them to rethink their position on the issue in question (which inoculated participants had just been warned about in the treatment message). The scale items included: nonthreatening/threatening, unintimidating/ intimidating, not harmful/harmful, safe/dangerous, and not risky/risky. The overall reliabilities of the threat scales were very good: .97 for handguns, .96 for marijuana, and .95 for gambling. The final measure involved responses to counterarguments, which participants listed following their exposure to inoculation materials during Phase 2. After indicating their attitudes about a message, respondents were asked to identify possible arguments that were contrary to their own position and to list potential responses to those arguments in the spaces provided, employing a technique similar to the thought-listing procedure developed by Petty, Wells, and Brock (1976). Responses were subsequently coded by three researchers. Coders were instructed to count declarative statements refuting specific counterarguments as answers. One unique idea per space which met this criteria was counted, using a scoring method similar to one used previously by Brock (1967) and Petty et al. (1976). Researchers spent approximately two hours discussing rating procedure, and then participated in supervised practice sessions. Inter-coder reliability was assessed using Rosenthal's effective reliability method (Rosenthal, 1987). Inter-coder reliability ratings for responses to arguments contrary to attitudes were: for handguns, .97; for marijuana, .98; and for gambling, .94. RESULTS Manipulation Checks Two manipulation checks were employed as a precondition to all subsequent analyses to confirm effectiveness of inoculation treatments and the presence of sufficient threat levels across the three topics/C Planned comparisons, employing Dunn's multiple comparison procedure (Kirk, 1982) were computed across the inoculation and control conditions on receiver attitude and threat on the three topics. Results confirmed the effectiveness of the inoculation and threat manipulations. The inoculated subjects demonstrated greater resistance to the influence of persuasive attacks across the topics of handguns, F(l,403) = 34.97, p < .001, eta2 = .09; marijuana, F(l,421) = 52.17, p < .001, eta2 = .13; and gambling, F(l,396) = 24.25, p < .001, eta2 = .06. In addition, inoculated subjects manifested higher threat levels for handguns, F(l,400) = 19.68, p < .001, eta2 = .05; marijuana, F(l,427) = 7.10, p < .05, eta2 = .02; and gambling, F(l,397) = 39.25, p < .001, eta2 = .10. Inoculation and threat means are depicted in Table 1. Nuances in Inoculation 469 TABLE 1 Summary of the Threat and Attitude toward the Attack Message Means among Inoculated and Control Subjects across the Handguns, Marijuana, and Gambling Content Areas Experimental Condition Dependent Measure Control Inoculation Treatments: Peripheral Central Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 HANDGUNS TOPIC Attitude Mean SD Threat Mean SD Attitude Mean SD Threat Mean SB Attitude Mean SD Threat Mean SD 5.08 (N=96) 2.17 417. (#=150) 2.12 4.21. (#=158) 2.08 4.05 (#=95) 2.10 4-73c (#=149) 2.30 MARIJUANA TOPIC 4.78c (#=157) 2.28 5.16 (#=100) 1.94 4.23, (#=156*) 1.94 4.08. (#=166) 2.03 3.82 (#=102) 1.98 4.13 (#=159) 2.13 GAMBLING TOPIC 4.35c (#=167) 2.36 5.27 (#=95) 1.78 4.46, (#=144) 1.86 4.74c (#=158) 2.04 3 -73 b (#=145) 1.84 3.81. (#=157) 1.84 2.99 (#=96) 1.56 *' Note: Ratings of respondent attitudes and threat were evaluated using 9-point scales. For the attitude measures, the lower therating, the more resistant to the attack message. However, for threat, a higher rating is associated with greater resistance. , Significant compared to control at g < .001. b Significant compared to control at g < .01. c Significant compared to control at g < .05. 470 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 Overall Findings A series of 3 (systematic inoculation treatment, peripheral inoculation treatment, and no inoculation/control) X 2 (lower and higher need for cognition) Multivariate Analysis of Covariance (MANCOVA) tests, using Wilk's lambda test, were computed on the dependent measures of attitude, responses to counterarguments, and receiver threat across each of the three topics as the first step in examining the predictions and research questions posited in this study. Ego-involvement was treated as a covariate in the analyses. In addition, 3X2 ANCOVAs were computed on attitude change scores on each of the three topics between Time 1 (which was prior to administration of inoculative materials) and Time 2 (immediately following administration of inoculative materials) and from Time 1 to Time 3 (from one to three weeks following administration of persuasive attack). Significant omnibus effects were followed by tests of simple effects, where needed, and subsequent assessment of means using planned comparisons for all predicted effects and Scheffe post-hoc tests for all nonpredicted effects. To maintain clarity in the reporting of the findings of this study, omnibus results are presented separately for each topic. Later, the relevant main and covariate effects for specific variables are examined in the context of appropriate predictions. The factorial MANCOVA indicated no interaction effects involving any of the three topics. MANCOCA results for the handguns topic revealed main effects for experimental condition, F(6,762) = 3.63, p < .01, R2 = .03, and nearly significant main effects on need for cognition, F(3,381) = 2.59, p < .06, R2 = .02. The factorial ANCOVA on Time 1 to Time 3 attitude change for the handguns topic revealed a main effect for experimental condition, F(2,388) = 3.35, p < .01, eta2 = .02. The factorial MANCOVA results for marijuana indicated main effects for experimental condition, F(6,820) = 4.39, p < .001, R2 = .03, and need for cognition, F(3,410) = 2.79, p < .05, R2 = .02. The factorial ANCOVA on Time 1 to Time 3 attitude change for marijuana indicated a main effect for experimental condition, F(2,402) = 5.56, p < .01, R2 = .02. The factorial MANCOVA for the gambling topic indicated a main effect involving experimental condition, F(6,756) = 3.73, p < .01, R2 = .03, while the ANCOVA on attitude change revealed a nearly significant main effect for experimental condition, F(2,384) = 2.33, p < .10, eta2 = .01. Hypotheses Hypotheses 1 and 2 examined use of central and peripheral inoculation appeals in conjunction with processing disposition. Hypothesis 1 posited that central inoculation treatments are more effective than peripheral treatments. Uncontaminated main effects for experimental condition across all three topic areas indicated that both central and peripheral inoculation appeals confer resistance to influence, but failed to reveal any statistically significant differences between the two approaches. Subsequent planned comparison for handguns indicated that both central and peripheral approaches instilled resistance on the dependent variable of attitude: central, F(l,349) = 12.92, p < .001, eta2 = .04, and peripheral F(l,349) = 11.81, p < .001, eta2 = .03. The pattern of results was the same for the marijuana and gambling topics. With both issues, central and peripheral appeals promoted resistance, but there were no significant differences between approaches. Subsequent planned comparisons on marijuana revealed main effects on attitude for central and peripheral approaches, Nuances in Inoculation 471 Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 F(l,383) = 15.44, p < .001, eta2 = .04, and F(l,383) = 20.82, p < .001, eta2 = .05, respectively. Planned comparisons for gambling indicated main effects on attitude for central and peripheral appeals, F(l,344) = 12.26, p < .001, eta2 = .04, and F(l,344) = 5.23, p < .05, eta2 = .02, respectively. This pattern of results suggests that central and peripheral inoculation treatments are similar in their ability to promote resistance to subsequent influence. These means are displayed in Table 1. Hypothesis 2 predicted that inoculation is more effective in conferring resistance among individuals who receive an inoculation treatment who are higher in need for cognition. Omnibus MANCOVA results reported previously revealed significant main effects on need for cognition with the handguns and marijuana topics. These results were probed with one-way MANOVAs examining receiver need for cognition among inoculated subjects. The pattern of means on need for cognition reveals that those individuals higher in need for cognition were somewhat more resistant to subsequent attacks, but mean differences attained statistical significance only with the higher involving handguns issue, F(l,295) = 4.21, p < .05, eta2 = .02. With the handguns topic, need for cognition triggered greater threat, F(l,295) = 5.10, p < .01, eta2 = .04, and, thus, more resistance to subsequent attacks. Need for cognition means on attitude among inoculated subjects are displayed in Table 2. The results suggest qualified support for Hypothesis 2. TABLE 2 Attitude toward Attack Message Means among Inoculated Subjects Scoring Lower and Higher in Need for Cognition across Handguns, Marijuana, and Gambling Content Areas Dependent Measure Need for Cognition Lower Higher HANDGUNS TOPIC Attitude Mean SD 4.46 (#=140) 2.03 3.94, (#=164) 2.12 MARIJUANA TOPIC Attitude Mean SD 4.29 (#=168) 1.96 3.99 (#=151) 2.03 GAMBLING TOPIC Attitude Mean SD 4.54 (#=141) 1.87 4.69 (#=158) 2.04 Note: Ratings of respondent attitudes were evaluated using 9-point scales. The lower the rating, the more resistant to the attack message. m Significant at r¿< .05. 472 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 Hypotheses 3,4, and 5 tested assimilation and contrast in the process of resistance. Specifically, Hypothesis 3 predicted that inoculation immediately enhances existing attitudes, while Hypothesis 4 posited that this tendency is most pronounced among more ego-involved receivers. However, omnibus ANCOVAs computed on attitude change scores from Time 1 (prior to inoculation) to Time 2 (immediately following inoculation) revealed no statistically significant shifts in attitudes on the handguns, marijuana, or gambling topics. It is clear that the inoculation process does not initially strengthen existing attitudes. Hypothesis 5 posited that inoculation subsequently instills resistance to persuasive attacks. Results reported previously (see Table 1) indicate that inoculation confers resistance to persuasive attacks. However, given the failure to confirm an assimilation effect immediately following the administration of inoculative materials, it would be inappropriate to describe the subsequent resistance provided as a contrast effect. However, the concept of ego-involvement is not restricted to use in social judgment theory. As reasoned previously, more ego-involvement facilitates access to relevant attitudes, thus increasing the likelihood that an individual can be induced to perceive potential vulnerability of attitudes and be motivated to extend the cognitive energy required to bolster attitudes. Thus, Hypothesis 6 posited that the tendency of inoculation treatments to generate threat is most pronounced among more egoinvolved receivers, and Hypothesis 7 predicted that inoculation effects are more pronounced among more highly ego-involved receivers. To assess Hypothesis 6, the influence of ego-involvement was examined for the main effect findings of experimental condition on the dependent variable of threat across the three topics. The results indicated that the covariate of ego-involvement attained statistical significance with the moderately involving marijuana topic, b = .34, t = 7.41, p < .001, and low involving gambling topic, b = .19, t = 3.80, p < .001. The positive betas indicate support for Hypothesis 6: the ability of inoculation treatments to generate threat is most pronounced among more ego-involved receivers. To evaluate Hypothesis 7, the influence of ego-involvement was examined for main effect findings of experimental condition on the remaining dependent variables across three topic areas. The pattern of results indicates no support for the prediction that inoculation is more effective among more ego-involved receivers. DISCUSSION The results of this investigation enhance understanding of the process of inoculation. The study used three controversial issues, representing low, moderate, and high issue involvement and a roughly equal distribution of opinion for and against each issue. The study addressed the potential of both central and peripheral inoculation treatments to confer resistance to attack, adjusting for receiver need for cognition, and explored the cognitive process triggered via inoculation. Hypotheses 1 and 2 examined inoculation appeals and receiver message processing disposition. Hypothesis 1 posited superiority of central as opposed to peripheral appeals. The results offered no support for this prediction, instead revealing that central and peripheral inoculation treatments are similar in ability to instill resistance to subsequent influence. This is the first test of central and peripheral message approaches in inoculation. The finding that both approaches are equally effective adds to past evidence Nuances in Inoculation 473 Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 suggesting a robustness of inoculation appeals, whether they are fashioned as same versus different (McGuire, 1961,1962,1966; McGuire & Papageorgis, 1961; Pfau, 1992; Pfau & Burgoon, 1988; Pfau et al., 1990), or content versus source (Freedman & Sears, 1965; Stone, 1969). One plausible explanation for this established pattern of null findings with regard to inoculation message approach is that the threatening component in inoculation overshadows refutational preemption in the process of resistance. Threat functions as the motivational catalyst to inoculation. It is accomplished at the outset of the inoculation treatment, followed by specific content for receivers to use to bolster attitudes. Wyer (1974) previously speculated that warning people that their beliefs may be attacked is sufficient to instill resistance to attacks. Wyer argued that mentioning "arguments against a proposition may be sufficient to make the subject aware of his vulnerability to attack and...may stimulate him to bolster—defenses" (1974, p. 206). This position is supported by the evidence indicating that inoculation confers resistance to completely different arguments than raised in the inoculation treatment, described previously, and by studies conducted by Freedman and Sears (1965) and Kiesler and Kiesler (1964) which found that forewarning alone is enough to facilitate resistance. In examining this thesis within the context of inoculation, McGuire and Papageorgis (1962) reported that forewarning without refutational preemption is sufficient to foster resistance, although the use of forewarning in combination with refutational preemption is better. If the primary force of inoculation stems from the threatening component of a treatment, then the contribution of the particular content provided in the refutational preemption facet of the message will be minimized, therefore reducing the likelihood that any structural or content differences in the presentation of the refutational material will prove to be significant Hypothesis 2 predicted that inoculation is more effective among receivers higher in need for cognition. Results provided qualified support for this prediction, indicating that greater need for cognition was associated with higher threat and, thus, enhanced resistance, but only with the higher involving handguns issue. The finding that receiver need for cognition influences the effectiveness of inoculation is useful to practitioners who need to tailor treatments to target audiences. The fact that greater need for cognition enhances the effectiveness of inoculation on high involving, but not low involving, issues makes theoretical sense. Issue involvement and receiver need for cognition both portend the presence of more effortful message processing, which contributes to the overall effectiveness of inoculation (Petty & Cacioppo, 1986; Pfau et al., 1997). Cognitive response research suggests that messages enhance attitude persistence by motivating receivers to generate their own thoughts about a topic (Cacioppo & Petty, 1989; Chaiken, Liberman, & Eagly, 1989; Eagly & Chaiken, 1993; Petty & Cacioppo, 1986). Thus, greater need for cognition should contribute to.the effectiveness of inoculation with high involving issues. The investigation focused on the cognitive process unleashed via inoculation. The study posited the processes of assimilation and contrast, found in social judgment theory (Sherif & Hovland, 1961; Sherif & Sherif, 1967; Shérif et al., 1965), as plausible explanations for the cognitive process that inoculation triggers in receivers. Hypotheses 3-5 addressed assimilation and contrast rationales, but the results of this 474 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 study provided no support for an assimilation effect or for an interaction of assimilation and ego-involvement Absent support for assimilation, it would be inappropriate to interpret the pervasive main effect findings for inoculation across the three topics as a contrast effect, as was reasoned in Hypothesis 5. The results of this investigation suggest that assimilation and contrast do not offer suitable explanations of the process of resistance. This finding confirms the reasoning of Petty, Cacioppo, and Haugtvedt (1992) that assimilation and contrast are not good process explanations for social judgment-like attitude movements. This study offered a robust test of assimilation and contrast in inoculation. We therefore conclude that a suitable rationale for the cognitive processes that are triggered via inoculation must lie elsewhere (Pfau, 1997). Further theoretical and empirical work needs to be undertaken to understand the nature of the internal processes set in motion by an inoculative treatment. Hypotheses 6 and 7 posited independent effects for the key term of egoinvolvement Results revealed support for Hypothesis 6, which predicted that the capacity of inoculation treatments to generate threat is most evident in high egoinvolved receivers, at least for the moderately involving marijuana and low involving gambling topics. The more ego-involved in a content area a person is, the more likely he/she can access relevant attitudes (Fazio, 1989) thereby facilitating threat, which requires that receivers perceive the potential vulnerability of attitudes. However, the results provided no support for Hypothesis 7, which posited that inoculation effects are most pronounced among more ego-involved receivers. Thus, greater egoinvolvement appears to contribute to the process of inoculation, enhancing threat, but does not directly affect resistance. Two caveats should be posited concerning the results of this study. First, the investigation featured central and peripheral inoculation treatments, but it made no attempt to manipulate or measure receiver message processing. As a result, the study is unable to confirm whether the the treatments triggered central or peripheral message processing in receivers. This shortcoming is compounded by the fact that, according to the ELM, central and peripheral cues are determined on the basis of their actual use (Petty & Cacioppo, 1986) The second limitation of the results of this study concerns the small effect sizes reported, which averaged .037 across all tests. In resistance research, however, small effects sizes are common and meaningful. To detect inoculation effects, attack messages administered to those receivers who support the opposing position must exert influence. Clearly, even successful attacks will only account for a small proportion of total variance in the attitudes of those committed to the opposing point of view. Also, this study employed a single inoculation message per subject, but when employed in the field, inoculation would be accomplished via multiple messages delivered over time (Pfau et al., 1990; Pfau et al., 1992), thus enhancing their effect. 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L. (1985). Measuring the involvement construct. Journal of Consumer Research, 12, 341-352. APPENDIX Attack messages were prepared for and against each of the three topics and administered to people opposing the position taken in the attack. Four inoculation messages were written for each of the attack messages, two central and two peripheral treatments. To illustrate, the examples included in the appendex feature an attack calling for legalization of marijuna, and four inoculation messages designed to promote resistance to this attack. 478 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang Attack Message Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 IT'S TIME TO LEGALIZE THE SALE A N D USE OF MARIJUANA Marijuana is a substance, and its use in the U. S. has become almost as common as that of other substances, like alcohol. Recent government data indicates that more than one in every five American teenagers and adults~37-million in all-have used marijuana. Given such extensive use, the time has finally come to officially legalize the sale and use of marijuana in the U. S. This step makes as much sense as the legalization of alcohol did more than 60 years ago. It would end the hypocrisy associated with unenforceable laws. Further, it would result in a number of important benefits! One benefit is medical. Marijuana has important medical uses. It is used to treat cancer chemotherapy patients. The prestigious Journal of the American Medical Association indicates that 200,000 Americans are receiving chemotherapy treatments to combat cancer. Of these, more than 50,000 experience severe nausea from treatments. Studies indicate that marijuana could assist about half this group to overcome nausea. Marijuana can also be employed to treat glaucoma, a serious eye disease. Mary Johnson, a glaucoma patient reports that, "Marijuana does save my eyes, and my ophthalmologist says there is no other medicine to prevent me from going blind." Finally, marijuana can be used to treat alcoholism. Tod Mikuriya, a prominent doctor, recommends marijuana for his alcoholic patients. Research shows that regular marijuana use can significantly lower consumption of alcohol. In addition to its medical applications, the legalization of the sale and use of marijuana could provide a financial bonanza, helping pay for needed public services, and reducing the national debt. The amount spent on marijuana is staggering. One estimate indicates that approximately $4 billion is spent annually on marijuana by users, and that more than 50 percent of this is profit to the seller. One U. S. Senator estimates that legalization of marijuana would be accompanied by a plan to tax its profits. He concluded that, "We could impose a tax similar to those on alcohol and cigarettes, which would generate significant public revenues." Lisa Smith, a taxpayer, observed the obvious. She said that, "If imposing a tax on marijuana could solve government's financial problems, and as a result improve our living standard, why not do it?" Since legalizing marijuana could bring so many benefits, why not do it? Just as government legalized alcohol more than 60 years ago, the time has come to legalize the sale and use of marijuana. It just makes sense! Inoculation: Refutational Same, Central D O N T LEGALIZE THE SALE AND USE OF MARIJUANA Marijuana is a harmful drug that should remain illegal. Despite this fact, there is a new campaign underway to legalize the sale and use of marijuana in the U. S. Some of the appeals of this campaign are so persuasive that they may cause you to question your opposition to legalization. Advocates for the legalization of marijuana claim the substance provides important medical benefits for people, especially for cancer patients who suffer from nausea following chemotherapy treatments. However, many studies show that marijuana provides only short-term antiemetic relief, and only for a minority of users. Instead of relief, more than 70% of the people studied experienced side effects, but received no benefits. Besides, for patients who would benefit medically, existing laws permit physicians to prescribe marijuana. Proponents of legalization also claim that this step can provide an important new source of government tax revenue, much like those derived from the sale of cigarettes and alcohol. But, this Nuances in Inoculation 479 advantage would be more than offset by the social costs of legalization. At the most, a tax would raise about $2-billion annually, according to those who advocate legalization. However, one recent health report placed the social pricetag of marijuana use at $10.3-billion per year, several times the social costs of cigarettes or alcohol. If the legalization of marijuana increases its use, which many studies maintain, the human cost would be tragic. Remember that marijuana is a harmful drug. Oppose efforts to legalize the sale and use of marijuana in the U. S. Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 Inoculation: Refutational Different, Central DONT LEGALIZE THE SALE AND USE OF MARIJUANA Marijuana is a harmful drug that should remain illegal. Despite this fact, there is a new campaign underway to legalize the sale and use of marijuana in the U. S. Some of the appeals of this campaign are so persuasive that they may cause you to question your opposition to legalization. Those who support the legalization of marijuana maintain that it would benefit America. What they ignore is the personal and economic devastation it would cause. On a personal level, marijuana use places the individual at risk. Marijuana use speeds heartbeat. One study found that use increases heartbeat significantly, in some cases to twice its normal levels. In economic terms, marijuana use costs the U. S. economy billions in lost revenues. Research indicates that drug use in the work place is widespread, and that marijuana use is the primary culprit. It slows motor response, and thus places workers at risk. Studies show that accidents involving machinery, including motor vehicles, are the main cause of death and injury in the work place, and that most of these are drug-related. Marijuana use also undermines worker productivity. A survey of 3,000 General Motors workers in 13 plants throughout the U. S. revealed that drug-dependent employees visited company medical clinics 15 times more often, and experienced more than twice as many occupational injuries, resulting in 25 more lost working days each year in disability leave. Remember that marijuana is a harmful drug. Oppose efforts to legalize the sale and use of marijuana in the U. S. Inoculation: Refutational Same, Peripheral DONT LEGALIZE THE SALE AND USE OF MARIJUANA Marijuana is a harmful drug that should remain illegal. Despite this fact, there is a new campaign underway to legalize the sale and use of marijuana in the U. S. Some of the appeals of this campaign are so persuasive that they may cause you to question' your opposition to legalization. Advocates for the legalization of marijuana claim that the drug provides important medical benefits for people, especially for cancer patients who suffer from nausea following chemotherapy therapy. But, according to prominent oncologist Dr. James Moertel, marijuana offers only shortterm antiemetic relief, and only for a small minority of patients. George Kagen, a cancer patient who used marijuana during his chemotherapy, adds that, "In my experience, the benefits of marijuana use, when they do occur, are immediately followed by side effects." Besides, for those patients who need to use marijuana for medical purposes, existing laws permit physicians to prescribe it. Proponents of legalization also claim that this step would provide an important new source of government tax revenue, much like those derived from cigarettes and alcohol. However, this advantage would be more than offset by the social cost of legalization. Jack Stiff, an expert on 480 Pfau, Tusing, Lee, Godbold, Koerner, Penaloza, Hong, and Yang adolescent problems, advises that, "The damage of marijuana to users, particularly adolescents, is unimaginable." Or, as Lulu Loughlin, the mother of a marijuana user, put it: "If I lose my son, nothing can repay that loss." Remember that marijuana is a harmful drug. Oppose efforts to legalize the sale and use of marijuana in the U. S. Downloaded by [University of Minnesota Libraries, Twin Cities] at 15:40 23 October 2012 Inoculation: Refutational Different, Peripheral DONT LEGALIZE THE SALE AND USE OF MARIJUANA Marijuana is a harmful drug that should remain illegal. Despite this fact, there is a new campaign underway to legalize the sale and use of marijuana in the U. S. Some of the appeals of this campaign are so persuasive that they may cause you to question your opposition to legalization. Those who support legalization of marijuana say it would benefit America. What they ignore is the personal and economic devastation it would cause. On a personal level, marijuana use places the individual at risk. Dr. Adam Sulkowski explains that marijuana use increases heart rate significantly, in some cases to twice its normal levels. In economic terms, marijuana use costs the economy billions in lost income.'Newsweekreports that drug use in the work place is widespread, and that marijuana use is the primary culprit. It slows motor response, thus placing workers at risk. William Taft, president of the Manufacturers' Association of Southern Connecticut, explains: "Marijuana slows reactions and therefore can cause accidents. An worker who is hurt is no longer able to participate in production." Former drug user John Carpenter explains, "I lost my fingers in an accident due to drug use, and I lost my job." Marijuana use also reduces worker productivity. Dr. Robert Carlton warns that, "If we don't wake up and address the disastrous and wide-ranging effects of drug use in the workplace, the U. S. is doomed to become a second-rate power." Remember that marijuana is a harmful drug. Oppose efforts to legalize the sale and use of marijuana in the U. S Nuances in Inoculation 481