Biol 4503 General Microbiology in Poland, Summer Lecture/laboratory Schedule Lecture 1. Introduction, history of microbiology 2. Comparison of cell structure of pro- and eukaryotic microorganisms Chapter 1, pp. 1-5, 7-23 Chapter 2, pp. 25-34 Chapter 3, pp. 48-51 “Brock: Biology of Microorganisms” 13thedition by Madigan, Martinko, Dunlap, and Clark, 2012, Benjamin Cummings Publishers. 3. Prokaryotic cell structure and function I 4. Prokaryotic cell structure and function II Chapter 3, pp. 47-84 5. Bacterial growth 6. Control of microbial growth Chapter 4, pp. 86-92 Chapter 5, pp. 118-149 Chapter 26, pp. 756-767 9. Bacterial genetics II 10. Genetic engineering Chapter 10, pp. 273- 286 Chapter 11, pp. 292-294, 300 - 302 Chapter 12, pp. 333-336 Study day Laboratory I. Laboratory safety and procedures, lab reports, dry and wet sterilization procedures, media preparation Techniques for the isolation and cultivation of microorganisms LR, MS “Microbiology: A Laboratory Manual”10 th edition by Cappuccino & Sherman, 2014, Benjamin Cummings Publishers. Read: Introduction, Laboratory safety, Laboratory protocol, Basic laboratory techniques for isolation, cultivation and characterization of microorganisms. exp. 2, pp.7-12 exp. 3, pp. 19-27 exp. 4, pp. 29-34 exp. 20 (with modifications), pp. 137-144 II. Microbial morphology: bacterial staining LR, MS Text pp. 35-42 Text pp. 55-57 exp. 8, pp. 59-63 exp. 10, pp.71-74 exp. 11, pp. 75-80 exp. 13, pp. 87-95 III. Physical and chemical agents LR, MS exp. 16, pp. 115-120 exp. 42, pp. 285-288 exp. 43, pp. 289-292 exp. 46, pp. 309-316 special exp. (phytoncides) V. Bacterial genetics II LR, MS exp. 58, pp. 389-396 exp. 59, pp. 397-406 Examination I (two hours). Lectures 1-10, Labs I-V 100 multiple choice questions + five (5) essay questions each worth 10 points Collection of laboratory reports for labs I-V VI. Bacterialgenetics III LR 11. Metabolic diversity of microorganisms I Reading results, labs IV and V 12. Metabolic diversity of microorganisms II Chapter 4, pp. 92-108 Chapter 13, pp 341-368 , 13. Applied and industrial microbiology 14. Microbiology of foods Chapter 15, pp. 412-427 15. Microbial evolution and systematics 16. Microbial diversity Chapter 16-19, pp. 446-583 VII. Biochemical activities of microorganisms I. LR, MS Microbiology of foods (set-up) exp. 15, pp. 105-114 exp. 22, pp. 155-160 exp. 24, pp. 167-170 exp. 25, pp. 171-182 exp. 27, pp. 187-190 exp. 30, pp. 201-204 exp. 32B, pp. 209-214 special experiments VIII. Biochemical activities of microorganisms II. LR, MS Reading results, lab VII Quiz 1 17. Microbial habitats 18. Nutrient cycles, bioremediation, symbiosis Chapter 23, pp. 669-697 Chapter 24, pp. 698-750 Study day IX. Microbiology of water and soil LR, MS exp. 49, pp. 329-336 exp. 51, pp. 345-350 Examination II (two hours). Lectures 11-18, Labs VI-XI 80 multiple choice questions + four (4) essay questions each worth 10 points Collection of laboratory reports for labs VI-IX 19. Essentials of immunology 20. Mechanisms of antimicrobial defense Chapter 28, pp. 816 – 825 Chapter 29, pp. 839 – 858 Chapter 30, pp. 860 – 864 21. Diagnostic immunology I 22. Diagnostic immunology II Chapter 28, pp. 826 – 830 Chapter 31,pp. 892 – 904 X. Mechanisms of innate immunity:phagocytosis, complement MS special exp. (phagocytosis) special exp.(complement) XI. Antigen-antibody interactions: precipitation, agglutination, neutralization LR, MS exp. 71, pp. 487-492 (precipitation) exp. 72, pp. 493-498 (agglutination) special exp. ( neutralization) XII. Medical microbiology I LR, MS exp. 44 A, pp. 293-296 exp. 65, pp. 447-452 exp. 66, pp. 453-461 + media XIII. Medical microbiology II MS XIV. Field trip – hospital - microbiology diagnostic laboratory 23. Microbial interactions with humans I 24. Microbial interactions with humans II Chapter 27, pp. 787-810 Quiz 2 25. Clinical and diagnostic microbiology I 26. Clinical and diagnostic microbiology II Chapter 31, pp. 879-891 Chapter 26, pp. 767-774 and 778-786 XV. Parasitic protozoa MS 27. Vectorborne and soilborne microbial exp. 35, pp. 229-236 pathogens Special exp. ELISA G.intestinalis 28. Waterborne and foodborne microbial diseases Chapter 34, pp. 981-1001 Chapter 35, pp.1012-1018 Chapter 36, pp. 1030-1040 Study day Examination III (two hours) Lectures 19-28, Labs X-XV 100 multiple choice questions + five (5) essay questions each worth 10 points Collection of laboratory reports for labs XI-XVI LR = Laboratory Report (s) required (11 LR x 10 points) MS = Manual Skills evaluation (14 sessions x 5 points each)