Name__________________________ SALINIZATION LAB INTRODUCTION In Australia, where the climate is dry and precipitation is feeble, farmers and cropland owners lose nearly a billion dollars a year due to degradation of the soil. This occurs because water-soluble salts accumulate in the upper-rooting zone, thus hindering the growth of crops by limiting their ability to take up water. The process of such is called salinization and is a profound threat to food security and the environment. Learning about salinization is important because it damages approximately one-tenth of the world’s irrigated land and reduces irrigated land areas by 1-2% each year. Salinization occurs where arid or semi-arid soil is present. In areas that experience an ample amount of rainfall, the development of saline soil is hindered due to leaching, which is the downward movement of salts. Salinization may be caused by either natural processes or because of human practices. The presence of soluble salts (such as sulfates of sodium, calcium, and magnesium), a high water table, a high rate of evaporation, and low annual rainfall are conditions that are necessary for the formation of salinization. Some natural landscape features allow dissolved salts to accumulate in water. Once this water evaporates, the salt concentration consequently increases. Thus, salinization can occur this way as well. Other natural sources of salts in soil include the decomposition of rocks and minerals through chemical weathering. Nearly all types of water (with the exception of natural rainfall) contain dissolved salts. Once plants use the water, they leave the salt content behind, allowing it to accumulate in the soil. The restriction of adequate water drainage within the soil can also contribute to salinization. This is because the water will eventually evaporate, leaving the salts behind to accumulate. Areas with irrigation are also vulnerable to salinization, for a higher water table carries salts to the surface because of high evaporation demand. Although potentially destructive, there are several ways to prevent and correct salinization. Provided that there is sufficient natural drainage, it might help to use a bit more water than plants actually need. This would reduce the salt concentration by leaching salts past the root zone and into the aquifers, which would then wash away the unwanted salinity. Various forms of drainage, such as ditches and underground pipes, can be constructed to take water with high levels of salt away. In the case where an area experiences extreme salinization and can no longer sustain agriculture, the land may be rehabilitated through flooding. Though costly, this method would successfully wash away superficial salts. One of the more practical ways to prevent salinization is through more efficient use of irrigation water. For example, drip irrigation, where a metered amount of water is fed to the area around plants, is better than surface irrigation, which could possibly cause salt contents to accumulate. Preventing the spread of salinization and reclaiming the lands that have already been substantially salted are crucial in securing the stability of the global economy and the Name__________________________ environment of the future. Salinization threatens approximately 10 percent of the world’s grain harvest alone. By learning about the process and effects of salinization, one will obtain a better understanding of the need to address the issue. Lab Objective To understand how salinization affects plant growth. Question How does varying levels of salt affect mung bean seed germination? Hypothesis Materials: Petri dishes Graduated Cylinders Flasks and Beakers for mixing solutions Distilled Water Paper Towels Tweezers for seed placement Labels Balances Mung bean seeds Procedure for making salt solutions HINT: 1 mL of water weighs 1 gram Group Salt Concentration 1 0% 2 .01% 3 .1 % 4 .5 % 5 1.0% 6 2.0% 7 3.0% 8 4.0% Amount Water (mL) Amount salt (g) 100 0 99 1 Procedure: 1. Set up 8 petri dishes with 2 paper towels cut to fit inside 2. Label the petri dishes with salt concentration and initials 3. Evenly space 10 mung bean seeds throughout your dish between the paper towels 4. Pour 10 mL of appropriate salt solution into each dish 5. Place dishes in appropriate place under grow lights 6. After three days count and record the number of seeds that germinated Name__________________________ Data: Salt Concentration 0% .01% .1 % .5 % 1.0% 2.0% 3.0% 4.0% G1 G2 G3 Mung Bean Seed Germination G4 G5 G6 G7 G8 Average Analysis: 1. How does increasing salt concentration affect seed germination? 2. Why does increasing salt concentration affect germination? 3. In agricultural lands, what causes salinization of soils? 4. What can be done to prevent the salinization of soils? 5. How might genetic engineering solve this problem? %