PATRICK SAI-CHEONG LEUNG
Division of Rheumatology & Allergy
Genome and Biomedical Science Facility, 6510
School of Medicine, University of California
Davis, California 95616
Tel: 530-754-4943
Fax: 530-754-6047
E mail: psleung@ucdavis.edu
Professional Experience:
09-2010
Chair, NIHES Autoimmune Workshop, Mechanisms Working group.
2010- present
Vice-President, International Association of Chinese Allergists and Immunologist.
11- 2008
Visiting Professor, National Defense University, Taiwan.
2005-present
Associate Adjunct Professor, The Chinese University of Hong Kong.
1998
Visiting Associate Fellow, Academia Sinica, Taiwan.
July 1995-pres
Associate Adjunct Professor in Department of Internal Medicine, University of
California Davis
Jan 1989-Jul 1995
Assistant Adjunct Professor in Department of Internal Medicine, University of
California, Davis.
Education:
Post-doctoral research fellow in Department of Internal Medicine, School of Medicine
University of California Davis 1986-88.
Ph.D. in Microbiology, University of California Davis. 1986.
Bachelor of Science (Honors) in Biology. The Chinese University of Hong Kong. 1980
Field of Specialization:
Molecular biology of autoimmune diseases and allergy.
Professional Activities:
Section Chair : Mechanism section, NIHES Autoimmune workshop, 2010.
Vice-President, International Association of Chinese Allergist and Immunologist.
Member, American Association for the Advancement of Science (AAAS)
Reviewer: Hepatology, Gastroenterology, J. Immunology, J. Allergy and Clinical Immunology, Clinical
and Experimental Allergy, Journal of Molecular Biology, Clinical and Molecular Allergy, Experimental
Biology and Medicine, Comparative Biochemistry and Physiology, Proceedings of the Society for
Experimental Biology and Medicine.
Editorial Board: Frontiers of Bioscience, J. of Social and Economic Issue of Biotechnology, J.
Allergy and Therapy.
International Advisory Board: Journal of Microbiology, Immunology and Infection.
Principal Investigator.
Funding Source: NIH
Title: Immunotherapy of murine PBC
Year: 2010-2012
Funding Source: Food Allergy and Anaphylactic Network
Title: Mimotope vaccine against shrimp tropomyosin.
Year: 2009-2012.
Funding Source: UCD Health System Research Award.
Title: Novosphingobium and primary biliary cirrhosis.
Year: 2004 – 2006.
Funding Source: International Life Science Institute: Allergy and Immunology Institute
1
Title: Immune repertoire of shellfish allergy.
Year: 1998-2002.
Funding Source: NIH
Title: Ig repertoire of combinatorial autoantibodies in PBC.
Year: 1993-1998.
Funding Source: CRCC
Title: Combinatorial antibodies to prostate carcinoma.
Year: 1993-1994.
Funding Source: Tobacco-Related Disease Research Program
Title: Tumor specific antibodies.
Year: 1991-1994.
Co-investigator.
Funding Source: NIH
Title: Mechanisms and pathogenesis of primary biliary cirrhosis.
Year: 2011-2016.
(renewal submitted June 2010).
Funding Source: NIH
Title: Xenobiotics and primary biliary cirrhosis.
Year: 2008-2013.
Funding Source: Cyanotech
Title: An open label cross-sectional analysis on the effects ofspirulina on anemia and
Immunosenescence.
Year: 2009 to 2010.
Funding Source: Dairy Management
Title: The effect of milk components on the immune response to pneunovax: a randomized
placebo-controlled clinical trial.
Year: 2007-2008.
Patent:
United States Patent (6,111,071)
2000
Title: Recombinant Fusion Protein Comprising PDC-E2, BCOADC-E2 and OGDC-E2
and uses thereof.
2
Scientific Papers:
2011 Rong, G., Zhong, R., Lleo, A., Leung, P.S., Bowlus, C.L., Yang, G.X., Yang, C.Y., Coppel, R.L.,
Ansari, A.A., Cuebas, D.A., Worman, H.J., Invernizzi, P., Gores, G.J., Norman, G., He, X.S., Gershwin,
M.E. Epithelial cell specificity and apotope recognition by serum autoantibodies in primary biliary
cirrhosis. Hepatology, In Press.
2011 Wu, S. J., Yang , Y.H., Tsuneyama, K., Leung, P.S., IIIarionov P., Gershwin, M.E., Chuang,
Y.H. Innate immunity and primary biliary cirrhosis: Activated invariant natural killer T cells exacerbate
murine autoimmune cholangitis and fibrosis. Hepatology 53:915-25.
2011 Shimoda, S., Harada, K., Niiro, H., Shirabe, K., Taketomi, A., Maehara, Y., Tsuneyama, K.,
Nakanuma, Y., Leung, P., Ansari, A.A., Gershwin, M.E., Akashi, K. Interaction between Toll-like receptors
and natural killer cells in the destruction of bile ducts in primary biliary cirrhosis. Hepatology 53:1270-81.
2011 Selmi, C., Leung, P. S., Fischer, L., German, B., Yang, C.Y., Kenny, T. P., Cysewski, G.R.,
Gershwin, M.E. The effects of Spirulina on anemia and immune function in senior citizens. Cell Mol
Immunol, In Press.
2011 Yang, G. X., Wu, Y., Tsukamoto, H., Leung, P. S., Lian, Z.X., Rainbow, D.B., Hunter, K.M.,
Morris, G.A., Lyons, P.A., Peterson, L.B., Wicker, L.S., Gershwin, M.E., Ridgway, W.M. CD8 T cells
mediate direct biliary ductule damage in nonobese diabetic autoimmune biliary disease. J. Immunol. 186:
1259-67.
2011 Stravitz, R.T., Lefkowitch, J.H., Fontana, R.J., Gershwin, M.E., Leung, P.S., Sterling, R.K., Manns,
M.P., Norman, G.L., Lee, W.M. Autoimmune acute liver failure: Proposed clinical and histological criteria.
Hepatology. 53: 517-26.
2010 Liu, H., Norman, G.L., Shums, Z., Worman, H.J., Krawitt, E.L. Bizzaro, N., Vergani, D., Bogdanos,
D.P., Dalekos, G.N., Milkiewicz, P., Czaja, A.J., Heathcote, E.J., Hirschfield, G.M., Tan, E.M., Miyachi, K.,
Bignotto, M., Battezzati, P.M., Lleo, A., Leung, P.S., Podda, M., Gershwin, M.E. and Invernizzi, P. PBC
Screen: an IgG/IgA dual isotype ELISA detecting multiple mitochondrial and nuclear autoantibodies
specific for primary biliary cirrhosis. J. Autoimmunity. 35: 436-42.
2010
Tsuda, M., Torgerson, T.R., Selmi, C., Gambineri, E., Carneiro-Sampaio, M., Mannurita, SC,
Leung, P.S C,., Norman, G.L. and Gershwin, M.E. The spectrum of autoantibodies in IPEX syndrome is
broad and includes anti-mitochondrial autoantibodies. J. Autoimmunity. 35:265-268.
2010
Freeman, S.L., Fischer, L., German, J.B., Leung, P.S., Prince, H., Selmi, C., Naguwa, S.M. and
Gershwin. M.E. Dairy proteins and the response to pneumovax in senior citizens: a randomized, doubleblind, placebo-controlled pilot study. Annals of N.Y. Acad. Sci. 1190:97-103.
2009
Padgett, K.A., Lan, R.Y., Leung, P.S.C., Lleo, A., Dawson, K., Pleiff, J., Mao, T.K., Coppel, R.L.,
Ansari, A.A. and Gershwin, M.E. Primary biliary cirrhosis is associated with altered hepatic microRNA
expression. J. Autoimmunity. 32: 246-53.
2009 Wakabayashi, K, Yoshida, K., Leung, P. S., Moritoki, Y., Yang, G. X., Tsuneyama, K., Lian, Z. X,
Hibi, T., Ansari, A. A, Wicker, L. S., Ridgway, W. M., Coppel, R.L., MacKay, I. and Gershwin, M.E.
Induction of autoimmune cholangitis in non-obese diabetic (NOD).1101 mice following a chemical
xenobiotic immunization. Clin. Exp. Immunol. 155: 577-86.
2008 Mattner, J., Savage, P.B., Leung, P., Oertelt, S.S., Wangm V., Trivedi, O., Scanlon, S.T.,
Pendem, K., Teyton, L, Hart, J., Ridgway, W.M., Wickerm L.S., Gershwin, M.E. and Bendelac, A. Liver
autoimmunity trigerred by mircrobial activation of natural killer T cells. Cell Host and Microbe. 3:305-15.
2008 Wakabayashi, K., Lian Z.X., Leung, P.S.C., Moritoki, Y. , Tsuneyama, K., Kurth, M.J. , Lam K.S.,
Yoshida, K., Yang, G.X., Hibi, T., Ansari, A.A., Ridgway, W.M., Coppel, R.L., Mackay, I.R. and Gershwin,
M.E. Loss of tolerance in C57/BL6 mice to the autoantigen PDC-E2 by a xenobiotic with ensuring biliary
ductular disease. Hepatology.48: 531-40. .
3
2008 Leung, P.S.C., Lee, Y.S., Tang, C.Y., Kung, W.Y., Chuang, Y.H., , Chiang, B.L., Fung, M.C. and
Chu, K.H. Induction of shrimp hypersensitivity in mice. International Archives of Allergy and
Immunology. 147:305-314.
2007 Leung, P.S.C., Rossaro, L., Davis.P.A., Park, O., Tanaka, A., Kikuchi, K., Miyakawa, H., Norman,
G.L., Lee, W., Gershwin, M.E. and the Acute Liver Failure Study Group. Antimitochondrial Antibodies in
Acute Liver Failure: Implications for Primary Biliary Cirrhosis. Hepatology. 46: 1436-42.
2007 Leung, P.S.C., Park, O., Tsuneyama, K., Kurth, M.J., Lam, K.S., Ansari, A.A., Coppel, R.L.
and Gershwin, M.E. Induction of primary biliary cirrhosis in guinea pigs following chemical immunization.
J. Immunol. 179:2651-7.
2007 Oertelt, S., Rieger, R., Selmi, C., Invernizzi, P., Ansari, A.A., Coppel, R.L., Podda, M., Leung,
P.S.C. and Gershwin, M.E. A sensitive bead assay for antimitochondrial antibodies : chipping away at
AMA negative primary biliary cirrhosis. Hepatology.45:659-65.
2006 Rieger, R., Leung, P.S.C., Jeddeloh, M.R., Kurth, M.J., Nantz, M.H., Lam, K.S., Barsky, D.,
Ansari, A.A., Coppel, R.L., Mackay, I.R. and Gershwin, M.E. Identification of 2-nonynoic acid, a cosmetic
component, as a potential trigger of primary biliary cirrhosis. J. Autoimmunity. 27:7-16.
2006 Irie, J., Wu, Y.M. Wicker, L.S., Rainbow, D., Nalesnik, M.K., Hirsch, R., Peterson, L., Peterson, L.,
Leung, P.S.C., Cheng C., MacKay, I., Gershwin, M.E. and Ridgway W.M. NOD.c3c4 congenic mice
develop autoimmune biliary disease that serologically and pathogenetically models human primary biliary
cirrhosis. J. Exp. Med. 203: 1209-19.
2005 Amano, K., P. S. Leung, R. Rieger, C. Quan, X. Wang, J. Marik, Y. F. Suen, M. J. Kurth, M. H.
Nantz, K. S. Ansari, M. Zeniya, E. Matsuura, R. L. Coppel, and M. E. Gershwin. Chemical xenobiotics and
mitochondrial antigens in primary biliary cirrhosis: identification of antibodies against a common
environmental, cosmetic and food additive, 2-octynoic acid. J Immunol. 174:5874-83.
2005 Padgett, K.A., Selmi, C., Kenny, T.P., Leung, P.S.C., Balkwill, D.L., Ansari A.A., Coppel, R.L. and
Gershwin, M.E. Phylogenetic and immunological definition of four lipoylated proteins from
Novosphingobium aromaticivorans, implications for primary biliary cirrhosis. J. Autoimmunity. 24: 209219.
2005 Kimura, Y., Selmi, C., Leung, P.S.C., Mao, T.K., Schauer, J., Watnik, M., Kuriyama, S., Nishioka,
M., Ansari, A.A., Coppel, R.L., Invernizzi, P., Podda, M. and Gershwin, M.E. Genetic polymorphisms
influencing xenobiotic metabolism and transport in patients with primary biliary cirrhosis. Hepatology
41:55-63.
2004 Matsumura, S., Van de Water, J., Leung, P., Odin, J.A., Yamamoto, K.M Gores, G.J., Mostov, K.,
Ansari, A., Coppel, R.L., Shiratori, Y. and Gershwin, M.E. Capase Induction by IgA antimitochondrial
antibody:IgA-mediated biliary injury in primary biliary cirrhosis. Hepatology. 39:1415-22.
2004 Amano, K., Leung, P.S.C., Xu, Q., Marik, J., Quan, C., Kurth, M.J., Nantz, M.H., Ansari, A.A.,
Lam, K.S., Zeniya, M., Coppel, R.L. and Gershwin, M.E. Xenobiotic-induced loss of tolerance in rabbits to
the mitochondrial autoantigen of primary biliary cirrhosis is reversible. J. Immunol. 172:6444-52.
2003 Selmi, C., Balkwill, D.L., Invernizzi, P., Ansari, A., Coppel, R.L., Podda, M., Leung, P.S., Kenny,
T.P., Van de Water, J, Nantz, M.H., Kurth, M.J. and Gershwin, M.E. Patients with primary biliary cirrhosis
react against a ubiquitous xenobiotic-metabolizing bacterium. Hepatology. 38:1250-7.
2003 Bruggraber, S.F.A., Leung, P.S.C. , Amano, K., Quan, C. , Kurth, M.J. ,. Nantz, M.H. , Benson,
G.D., Van de Water, J. , Luketic, V., Roche, T. , Ansari, A., Coppel, R.L., and Gershwin, M.E.
Autoreactivity to lipoate and a conjugated form of lipoate as hapten and hapten/carrier in primary biliary
cirrhosis. Gastroenterology.125:1705-13.
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2003 Leung, P.S.C., Park, O., Matsumura, S., Ansari, A., Coppel, R.L. and Gershwin, M.E. Is there a
relation between Chlamydia infection and primary biliary cirrhosis. Clinical and Developmental
Immunology. 10: 227-233.
2003 Leung, P.S.C., Quan, C.,Park, O., Van de Water, J., Kruth, M., Nantz, M., Ansari, A., Coppel,
R.L. Lam, K.S. and Gershwin, M.E. 2003. Immunization with a xenobiotic 6-bromohexanoate BSA
conjugate break tolerance to mitochondrial autoantigens. J. Immunol. 170: 5326-32.
2002 Fukushima, N., Nalbandian, G., Van De Water, J., White, K., Ansari, A. A., Leung, P., Kenny, T.,
Kamita, S. G., Hammock, B. D., Coppel, R. L., Stevenson, F., Ishibashi, H. , Gershwin, M. E.
Characterization of recombinant monoclonal IgA anti-PDC-E2 autoantibodies derived from patients with
PBC. Hepatology. 36:1383-92.
2002 Kimura, Y., Leung. P.S.C., Kenny, T.P., Van de Water, J., Nishioka, M., Giraud, A.S., Neuberger,
J., Ansari, A., Coppel, R.L. and Gershwin, M.E. Differential Expression of Intestinal trefoil factor (ITF) in
biliary epithelial cells of primary biliary cirrhosis. Hepatology. 36:1227-35.
2001 Leung, P.S.C and Chu, K.H. 2001. cDNA cloning and molecular identification of the major oyster
allergen from the Pacific oyster Crassostrea gigas. Clin and Exp Allergy. 31:1287-94.
2001 Leung, J.W., Liu, Y.L., Leung, P.S.C., Chan, R.C.Y., Inciardi, J.F., Cheng, A.F. Expression of
bacterial -glucuronidase in human bile- an in vito study. Gastrointestinal endoscopy. 54:346-50.
2001 Chia, S.C., Leung, P.S.C., Liao, C.P., Huang, J.H. and Lee, S.H. Fragment of Japanese
encephalitis virus protein produced in Escherichia coli protects mice from virus challenge. Microbial
Pathogenesis 31:9-19.
2001 Tanaka, A., Leung, P.S.C., Kenny, T.P. Au-Young, J., Prindiville,T. Coppel, R.L., Ansari, A.A. and
Gershwin, M.E. Genomic analysis of differentially expressed genes in liver and biliary epithelial cells of
patients with primary biliary cirrhosis. J. Autoimmunity. 17:89-98.
2000 Gershwin, M.E., Leung, P.S.C., Hongzhe Li, Seldin, M.F. Primary biliary cirrhosis and
autoimmunity: evaluating the genetic risk. IMAJ. 2 (Suppl2):7-10.
2000 Tanaka, A., Nalbandian, G., Leung, P.S.C., Benson, G., Santiago, M., Findor, J.A., Branch, A.D.,
Coppel, R.L., Ansaor, A,A. and Gershwin, M.E. Mucosal immunity and primary biliary cirrhosis: presence
of antimitochondrial antibodies in urine. Hepatology. 32:910-5.
2000 Chu, K.H., Wong, S.H. and Leung, P.S.C. Tropomyosin is the major mollusk allergen: RT-PCR,
expression and IgE reactivity. Marine Biotechnology. 2:499-509.
2000 Sasaki, M., Ansari, A., Pumford, N., Van de Water, J. Leung, P.S.C., Humphries, K.M., Szweda,
L.I., Nakanuma, Y., Roche, T., Coppel, R.L., Gershwin, M.E. Comparative immunoreactivity of antitrifluoracetyl (TFA) antibody and anti-lipoic acid antibody in primary biliary cirrhosis: searching for a
mimic. J Autoimmunity. 15:51-60.
2000 Reynoso-Paz, S., Leung, P.S.C., Van de Water, J., Tanaka, A., Munoz, S., Bass, N., Lindor, K.,
Donald, P.J., Coppel, R.L., Ansari, A.A. and Gershwin, M.E. Evidence for a locally driven mucosal
response and the presence of mitochondrial antigens in saliva in primary biliary cirrhosis. Hepatology.
31:24-9.
1999 Dubel, L., Tanaka, A. Leung, P.S.C. Van de Water, J., Roche, T., Coppel, R and Gershwin, M.E.
Autoepitope mapping and reactivity of autoantibodies to the dihydrolipoamide dehydrogenase binding
protein of the human pyruvate dehydrogenase complex. Hepatology 29:1013-8.
1998 Leung, P.S.C., Chen, Y.C., M. Eric Gershwin, Wong, S.H., Kwan, H.S. and Chu, K.H.
Identification and Molecular Characterization of Charybdis feriatus Tropomyosin: the Major Crab Allergen.
J. Allergy Clin. Immunol. 102: 847-852.
5
1998 Malmborg, A.C., Shultz, D.B., Luton, F., Mostov, K.E., Richly, R., Leung, P.S.C., Benson, G.D.,
Ansari, A.A., Coppel, R.L., Gershwin, M.E. and Van de Water, J. Penetration and co-localization in MDCK
cell mitochondria of IgA derived from patients with primary biliary cirrhosis. J. Autoimmunity 11:573-80.
1998 Migliaccio, C., Nishio, A., Van de Water, J., Ansari, A.A., Leung, P.S.C., Nakanuma, Y., Coppel,
R.L. and M. Eric Gershwin. Monoclonal antibodies to mitochondrial E2components define autoepitopes of
a molecular mimic in primary biliary cirrhosis. J. Immunol. 161:5157-5163.
1998 Masanaga, T., Watanabe, Y., Van de Water, J., Leung, P.S.C., Nakanishi, T., Kajiyama, G.,
Ruebner, B.H., Coppel, R.L. and Gershwin, M.E. Induction and persistence of immune-mediated
cholangiohepatitis in neonatally thymectomized mice. Clin. Immunol. Immunopathol. 89:141-149.
1998 Mattalia, A., Quaranta, S., Leung, P.S.C., Bauducci, M., Van de Water, J., Calvo, P.L., Danielle,
F., Rizzetto, M., Ansari, A., Coppel, R. L., Rosina, F., and Gershwin, M.E. Characterization of
antimitochondrial antibodies in healthy adults. Hepatology. 27:656-661.
1998 Nakanuma, Y., Harada, K., Kaji, K., Terasaki, S., Tsuneyama, K., Moteki, S., Van de Water, J.,
Leung, P.S.C. and Gershwin, M.E. Clinicopathological study of primary biliary cirrhosis negative for
antimitochondrial antibodies. Liver. 17:281-287.
1998 Leung, P.S.C., Chen, Y.C., Mykles, D.L., Chow, W.K., Li, C.P., and Chu, K.H. Molecular
identification of the lobster muscle protein tropomyosin as a seafood allergen. Molecular Marine Biology
and Biotechnology 7(1):12-20.
1998 Leung, P.S.C. and Chu, K-H. Molecular and immunological characterization of shellfish allergens.
Frontiers in Bioscience 3, d306-312.
1997 Van Norstrand, M.D., Malinchoc, M., Lindor, K.D., Therneau, T.M., Gershwin, M.E., Leung,
P.S.C., Dickson, R. and Homburger, H.A. Quantitiative measurement of autoantibodies to recombinant
mitochondrial antigens in patients with primary biliary cirrhosis : reationship of levels of autoantibodeis to
disease progression. Hepatology 25:6-11.
1997 Nishio, A., Van de Waterm J., Leung, P.S.C., Joplin, R., Neuberger, J., Lake, J., Bjorkland, A.,
Totterman, T.H., Peters, M., Worman, H.J., Ansari, A.A., Coppel, R.L., and Gershwin, M.E. Comparitive
studies of antimitochondrial autoantibodies in sera and bile in primary bilairy cirrhosis. Hepatology
25:1085-1089.
1996 Moteki, S., Leung, P.S.C., Coppel, R.L., Dickson, E.R., Kaplan, M., Munoz, S. and Gershwin,
M.E. Use of a designer triple expression hybrid clone for three different lipoyl domains for the detection of
anti-mitochondrial antibody. Hepatology 24:97-103.
1996 Cha, S., Leung, P.S.C., Van de water, J., Tsuneyama, K., Joplin, R.E., Ansari, A.A., Nakanuma,
Y., Schatz, P.J., Cwirla, S., Labris, L.E., Neuberger, J.M., Gershwin, M.E. and Coppel, R.L. Random
Phage Minotopes recognized by monoclonal antibodies against the pyruvate dehydrogenase complex-e2.
(PDC-E2). Proc. Natl. Acad. Sci. 93:10949-54.
1996 Leung, P.S.C., Chow W.K., Duffey, S., Gershwin, M.E. and Chu, K.H. IgE reactivity against a
cross-reactive allergen in crustacea and mollusca: evidence for tropomyosin as the common allergen. J.
Allergy Clin. Immunol. 98:954-961.
1996 Leung, P.S.C., Van de Water, J., Coppel, R.L., Nakanuma, Y., Munoz, S. and Gershwin, M.E.
Molecular aspects and the pathological basis of primary biliary cirrhosis. J. Autoimmunity 9:119-128.
1996 Moteki, S., Leung, P.S.C., Dickson, E.R., Van Thiel, D.H., Galperin, C., Buch, T., AlarconSegovia, D., Kershenobich, D., Kawano, K., Coppel, R.L., Matuda, S. and Gershwin, M.E. Epitope
mapping and reactivity of autoantibodies to the E-2 component of 2-oxo-glutarate dehydrogenase
complex (OGDC-E2) in primary biliary cirrhosis using recombinant OGDC-E2. Hepatology 23:436-444.
1996 Galperin, C., Leung, P.S.C. and Gershwin, M.E. Molecular Biology of Autoantigens in Rheumatic
Diseases. In Rheumatology Disease Clinics of North America. Vol. 22., No.1 Calabrese L.H. (ed). W.B.
Saunders Company.
6
1995 Leung, P.S.C., Chuang, D.T., Wynn, R.M., Cha, S., Danner, D.J., Ansari, A., Coppel, R. and
Gershwin, M.E. Autoantibodies to BCOADC-E2 in patients with primary biliary cirrhosis recognize a
conformational epitope. Hepatology 22:505-513
1995 Tsuneyama, K., Van de Water, J., Leung, P.S.C., Cha, S., Nakanuma,Y., Kaplan, M., De Lellis,
R. , Coppel, R., Ansari, A. and Gershwin, M.E. Abnormal expression of PDC-E2 on the luminal surface of
biliary epithelium occurs before MHC class II and BB1/B7 expression. Hepatology 21:1031-1037.
1995 Leung, P.S.C., Manns, M.P. and Gershwin, M.E. Inflammatory hepatobiliary cirrhosis. In: Clinical
Immunology: Principles and Practice. Vol. II., pp.1429-1443. Rich R.R. (ed). Mosby-Year Book, Inc. St.
Louis, Missouri.
1994 Ansari, A., Neckelmann, N., Villinger, F, Leung, P., Danner, D.J., Brar, S., Zhao, S, Gravanis,
M.B., Mayne, A., Gershwin, M.E. and Herskowitz, A. Epitope mapping of the branched chain alpha-keto
acid dehydrogenase E2 (BCKD-E2) that reacts with sera from patients with idiopathic dilated
cardiomyopathy. J. Immunol. 153:4754-4765.
1994 Leung, P.S.C., Chu, K.H., Chow, W.K., Ansari, A., Bandea, C., Kwan, H.S., Nagy, S.M. and
Gershwin, M.E. Cloning, expression and primary structure determination of Metapenaeus ensis
tropomyosin, the major heat stable shrimp allergen. J. Allergy Clin. Immunology. 94:882-890.
1994 Cha, S., Leung, P.S.C., Coppel, R.L., Van de Water, J., Ansari, A. and Gershwin, M.E.
Heterogeneity of combinatorial human autoantibodies against PDC-E2 and biliary epithelial cells in
patients with primary biliary cirrhosis. Hepatology 20:574-483.
1994 Pascual, V., Cha, S., Gershwin, M.E., Capra, J.D. and Leung, P.S.C. Nucleotide sequence
analysis of natural and combinatorial PDC-E2 antibodies in patients with primary biliary cirrhosis:
Recapitulating immune selection with molecular biology. J. Immunol. 152:2577-2585.
1994 Leung, P.S.C. , Mackay, I. and Gershwin, M.E. Mitochondrial Autoantigens. In Manual of
Biological Markers of Disease Vol. II. van Venrooji W.J. and Maini R.N. (eds)., B8.1:1-14 Kluwer
Academic Publishers, The Netherlands.
1993 Gershwin, M.E., Van de Water, J., Leung, P.S.C. and Coppel, R. T cell biology and cell surface
expression of PDC-E2 in primary biliary cirrhosis. In: Immunology and Liver. pp. 373-382. Johnstone, P
(ed)., Kluwer Academic Publishers.
1993 Cha, S., Leung, P.S.C., Keen, C.L., Gershwin, M.E., Gupta, R.S. and Fletcher, M.P. Effects of
zinc deprivation on heat shock proteins (hsp)-65 and -70 gene expression in pregnant rats. Nutrition
Research 13:1077-1086.
1993 Turchany, J., Leung, P.S.C., Jefferson, D., Ishida, J., Yamaguchi, M., Munoz, S., Danner D. and
Gershwin, M.E. Comparative metabolism and structure of BCKD-E2 in PBC. J. Autoimmunity. 6:459-466.
1993 Leung, P.S.C., Halpern, G.M. and Gershwin, M.E. Evaluation of possible histamine release from
human peripheral blood cells using an enzyme immunoassay (HRT) with components of intravenous
catheters. Allergie et Immunologie 25:346-353.
1993 Leung, P.S.C. and Gershwin, M.E. Immunoglobulin genes in autoimmunity. Int. Arch. Allergy
Immunol. 101:113-118.
1993 Van de Water, J., Turchany, J., Leung, P.S.C., Lake, J., Munoz, S., Surh, C.D., Coppel, R.,
Ansari, A., Nakanuma, Y. and Gershwin, M.E. Molecular mimicry in primary biliary cirrhosis: evidence for
biliary expression of a protein cross reactive with PDC-E2. J. Clin. Invest. 91:2653-2664.
1993 Leung, P.S.C., Watanabe, Y., Munoz, S., Patel, M.S., Korenberg, J.R., Hara, P., Coppel, R. and
Gershwin, M.E. Chromosomal localization and RFLP analysis of PDC-E2: the major autoantigen of
primary biliary cirrhosis. Autoimmunity 14:335-340.
7
1993 Leung, P.S.C. and Gershwin, M.E. Cloning and identification of autoantigens. Immunomethods
1:149-157.
1993 Cha, S., Leung, P.S.C., Fletcher, M.P., Ansari, A.A., Coppel, R.L. and Gershwin, M.E.
Combinatorial autoantibodies to dihydrolipoamide acetyltransferase, the major autoantigen of primary
biliary cirrhosis. Proc. Natl. Acad. Sci. USA. 90:2527-2531.
1992 Leung, P.S.C., Krams, S., Ansari, A., Kenny, T., Robbins, D.L., Fung, F., Starzl, T.E., Coppel,
R.L. and Gershwin, M.E. Characterization and epitope mapping of human monoclonal antibodies to PDCE2, the immunodominant autoantigen of primary biliary cirrhosis. J. Autoimmunity. 5:703-718.
1992 Iwayama, T., Leung, P.S.C., Nishioka, M, Nakagawa, T., Dickson, R., Coppel, R. and Gershwin,
M.E. Comparative immunoreactive profiles of Japanese and American patients with PBC against
mitochondrial autoantigens. Int. Arch. Allergy Immunol. 99:28-33.
1992 Caldwell, S.H. , Leung, P.S.C., Prindiville, T., Spivey, J.R., DeMedina, M., Saicheur, T., Reddy,
K.R., Rowley, M., Coppel, R.L., Jeffers, L.J., Mackay, I.R., Schiff, E.R. and Gershwin, M.E.
Antimitochondrial antibodies in kindreds of patients with primary biliary cirrhosis. Antimitochondrial
antibodies are unique to clinical disease and are absent in asymptomatic family members. Hepatology
16:899-905.
1992 Davis, P.A., Leung, P.S.C., Manns, M., Kaplan, M., Munoz, S.J., Gorin, F.A., Dickson, E.R.,
Krawitt, E., Coppel, R. and Gershwin, M.E. M4 and M9 antibodies in the overlap syndrome of primary
biliary cirrhosis and chronic active hepatitis: epitopes or epiphenomena? Hepatology 16:1128-1136.
1992 Leung, P.S.C., Iwayama, T., Prindiville, T., Chuang, D.T., Ansari, A.A., Wynn, R.M., Dickson, R.,
Coppel, R. and Gershwin, M.E. 1992. Use of designer recombinant mitochondrial antigens in the
diagnosis of primary biliary cirrhosis. Hepatology 15:367-372.
1992 Gershwin, M.E. , Rowley, M, Davis, P.A., Leung, P., Coppel, R. and Mackay, I.R. Molecular
biology of the 2-oxo-acid dehydrogenase complexes and anti-mitochondrial antibodies. In: Progress in
Liver Diseases, pp.47-61.
1991 Teuber, S., Coppel, R., Ansari, A.A., Leung, P.S.C., Neve, R., Mackay, I.R., Gershwin, M.E. The
identification and cloning of the murine genes encoding for the liver specific F alloantigens. J.
Autoimmunity 4: 857-870.
1991 Iwayama, T., Leung, P.S.C., Coppel, R.L., Roche, T.E., Patel, M.S., Mizushima, Y., Nakagawa,
T., Dickson, R. and Gershwin, M.E. Specific reactivity of recombinant human PDC-E1 in primary biliary
cirrhosis. J. Autoimmunity 4:769-778.
1991 Leung, P.S.C., Van de Water, J., Coppel, R.L. and Gershwin, M.E. Molecular characterization of
the mitochondrial autoantigens in primary biliary cirrhosis. Immunologic Research 10:518-527.
1991 Leung, P.S.C. and Gershwin, M.E. The immunobiology of heat shock proteins. J. Invest. Allergol.
Clin. Immunol. 1(1):23-30.
1990 Leung, P.S.C., Iwayama, T., Coppel, R.L. and Gershwin, M.E. Site directed mutagenesis of lysine
within the immunodominant autoepitope of PDC-E2. Hepatology 12:1321-1328.
1990 Fujikura, S., Davis, P., Prindiville, T., Leung, P., Fox, R. and Gershwin, M.E. Autoantibodies to
purified mitochondrial 2-oxo acid dehydrogenase in patients with Sjogren's Syndrome. J. Rheumatol.
17:1453-1457.
1990 Leung, P.S.C. and Gershwin, M.E. Molecular structures of autoantigens. Current Opinion
Immunol. 4(2):567-575.
1989 Van de Water, J., Surh, C., Leung, P.S.C., Krams, S.M., Fregeau, D., Davis, P., Coppel. R.,
Mackay, I.R. and Gershwin, M.E. Molecular definitions, autoepitopes and enzymatic activities of the
mitochondrial autoantigens of primary biliary cirrhosis. Seminars in Liver Diseases 9(2):132-137.
8
1988 Van de Water, J., Fregeau, D., Davis, P., Ansari, A., Danner, D., Leung, P., Coppel, R. and
Gershwin, M.E. Antibodies of primary biliary cirrhosis recognize dihydrolipoamide acetyltransferase and
inhibit enzyme function. J. Immunol. 141:2321-2324.
1988 Van de Water, J., Gershwin, M.E., Leung, P., Ansari, A. and Coppel, R.L. The autoepitope of the
74kd mitochondrial autoantigen of primary biliary cirrhosis corresponds to the functional site of
dihydrolipoamide acetyltransferase. J. Exp. Med. 167:1791-1799.
1988 Leung, S.C.P., Gershwin, M.E., Coppel, R., Halpern, G., Novey, H. and Castles, J.J. The
localization, molecular weight and immunological subclass response to A. fumigatus in acute
bronchopulmonary aspergillosis. Intl. Arch. Allergy Appl. Immunol. 85:416-421.
1988 Fregeau, D.R., Leung, S.C.P., Coppel, R.L., McNeilage, L.J., Medsger, T.A. and Gershwin, M.E.
Autoantibodies to mitochondria in systemic sclerosis: frequency and characterization using recombinant
cloned antigen. Arthr. Rheum. 31:386-392.
1988 Surh, C.D., Cooper, A.E., Coppel, R.L., Leung, P., Dickson, R. and Gershwin, M.E. The
predominance of IgG3 and IgM isotype antimitochondrial autoantibodies against recombinant fused
mitochondrial polypeptide. Hepatology 8:290-295.
1987 Leung, S.C.P. and Preiss, J. Cloning of the ADP glucose pyrophosphorylase (glgC) and glycogen
synthase (glgA) structural genes from Salmonella typhimurium LT2. J. Bacteriol. 169:4349-4354.
1987 Leung, S.C.P. and Preiss, J. Biosynthesis of bacterial glycogen: primary structure of Salmonella
typhimurium ADPglucose synthetase as deduced from the nucleotide sequence of glgC gene. J.
Bacteriol. 169:4355-4360.
1986 Leung, P., Lee, Y.M., Greenberg, E., Esch, K., Boylan, S. and Preiss, J. Cloning and expression
of the Escherichia coli glgC gene from a mutant containing an ADPglucose pyrophosphorylase with
altered allosteric properties. J. Bacteriol. 167:82-88.
1986 Chan, K.Y. and Leung, S.C. Effects of paraquat and glycophosphate on growth, respiration and
enzyme activity of aquatic bacteria. Bull. Environ. Contam. Toxicol. 36:52-59.
1983 Urbanowski, J., Leung, P., Weissbach, H. and Preiss, J. The in vitro expression of the gene for
Escherichia coli ADPglucose pyrophosphorylase is stimulated by cyclic AMP and the cyclic AMP receptor
protein. J. Biol. Chem. 258:2782-2784.
Manuscript submitted:
Naiyanetr, P., Butler, J.D. , Meng, L. , Pfeiff, J. , Kenny, T.P., Lam, K. , Kurth, M.,. Ansari, A.A., Coppel,
R.L., Gershwin, M.E and Leung, P.S.C. Electrophile-modified Lipoic Derivatives of PDC-E2 Elicits Antimitochondrial Antibody Reactivity. J. Autoimmunity.
Leung, P.S.C., Yang, G.X., Dhirapong, A., Tsuneyama, K., Ridgway, W. and Gershwin, M.E. Animal
Models of Primary Biliary Cirrhosis: Materials and Methods. In 2nd edition of Autoimmunity: Methods and
Protocols.
Manuscript in Preparation.
Leung, P.S.C., Tao, M.H., Naiyanetr, P., Chen, R., Purewal, S., Butler, J.D., Lam, K., Kurth, M. Specific
antibody reactivity to the 6,8-bis(acetylthio) octanoic acid moiety hapten in patients with primary biliary
cirrhosis.
Reviews
9
Leung, P.S.C., Shu S.A., Kenny, T.P., Wu, P.Y. and Tao, M.H. 2010 Development and
validation of gene therapy in autoimmune diseases: Epidemiology to animal models. Autoimm
Rev 9:A400-405. (Invited Review)
Leung, P.S.C., Dhirapong, A., Wu, P.Y. and Tao, M.H. 2010 Gene therapy in
autoimmune diseases: Challenges and opportunities. Autoimm Rev 9:170-74. (Invited
Review)
Dhirapong, A., Lleo, A., Leung, P. , Gershwin, M.E., Liu, F.T. 2009. The immunological potential
of galectin-1 and -3. Autoimmunity Reviews 8: 360-3.
Leung, P.S.C. , Coppel, R.L. and M. Eric Gershwin. 2005. Etiology of primary biliary cirrhosis.
Seminar in Liver Diseases.25:327-336. (Invited Review)
Lan, R., P. Leung, A. Ansari, R. Coppel and M.E. Gershwin. 2005. Solving the primary biliary
cirrhosis puzzle: The emerging image of immunopathology in primary biliary cirrhosis. Clinical and
Applied Immunology Reviews 5:271-284.
Yasnori, I., Leung, P.S.C., Ishibashi, H., Coppel, R.L., Ansari, A.A. and Gershwin, M.E. 2004.
Mitochondria and autoimmunity in primary biliary cirrhosis. Mitochondrion 4:743-53.
Tang, C.Y., Leung, P.S.C., Chiang, B.L. and Chu, K.H. 2003. Animal models of food allergy.
Recent Res Devel Alllergy and Clin Immunol 4:1-14.
Leung, P.S.C. and Chu, k.H. Current molecular immunological perspectives on seafood
allergies. 2001. Recent Res Devel Alllergy and Clin Immunol. 2:183-195. (Invited Review)
Besler, M. Daul, C.B., Leung, P.S.C.
Allergen Data Collection
<http://www.food-allergens.de/symposium-3-1/shrimps/shrimps-allergens.html>
Shrimps (Natantia) Internet Symposium on Food Allergens .Volume 3 Number 1
January - March 2001.
Leung, P.S.C., Chen Y.C. and K.H. Chu. 1999. Seafood Allergy: tropomyosins and beyond. J.
Microbiology Immunol Infection. 32:143-54.
Leung, P.S.C. and Chu K.H. 1998. Molecular and immunological characterization of shellfish
allergens. Frontiers in Bioscience. 3:306-312. (Invited Review)
Leung, P.S.C., Coppel R.L., Ansari, A., Munoz, S. and Gershwin, M.E. 1997. Antimitochondrial
antibodies in primary biliary cirrhosis. Semin. Liv. Dis. 17:61-69. (Invited Review)
Leung, P.S.C. Van de Water, J., Coppel, R.L., Nakanuma, Y., Munoz, S. and Gershwin, M.E. 1996.
Molecular aspects and the pathological basis of primary biliary cirrhosis. J. Autoimmunity 9:119-128.
Leung, P.S.C. and Gershwin, M.E. Immunoglobulin genes in autoimmunity. 1993. Int. Arch.
Allergy Immunol. 101:113-118.
Leung, P.S.C., Van de Water, J., Coppel, R.L. and Gershwin, M.E. 1991 Molecular characterization
of the mitochondrial autoantigens in primary biliary cirrhosis. Immunologic Research 10:518-527.
Leung, P.S.C. and Gershwin, M.E. 1991 The immunobiology of heat shock proteins. J. Invest
Allergol Clin Immunol. 1(1):23-30.
Leung, P.S.C. and Gershwin, M.E. 1990 Molecular structures of autoantigens. Current Opinion in
Immunol. 4(2):567-575.
Van de Water, J., Surh, C., Leung, P.S.C., Krams, S.M., Fregeau, D., Davis, P., Coppel. R.,
Mackay, I.R. and Gershwin, M.E. 1989. Molecular definitions, autoepitopes and enzymatic activities of
the mitochondrial autoantigens of primary biliary cirrhosis. Seminars in Liver Diseases 9(2): 132-137.
10
Book Chapters
Leung, P.S.C. and Gershwin, M.E. 2006. Native and Recombinant Autoantigens. In:
Autoantibodies. Peter, J.B., and Shoenfeld, Y. (eds.), pp.37-45. Elsevier Science Publishers.
Chu, K.H. Tang, C.Y., Wu, A. and Leung, P.S.C. 2005 Current perspectives on seafood allergy. In:
Ulber, R. and Le Gal, Y. (ed.) Advances in Biochemical Engineering/Biotechnology Vol. 97. Marine
Biotechnology II, pp. 205-235. Springer Verlag, Berlin.
Gershwin, M.E., P.S.C. Leung, J. Van de Water, A. Ansari, R.L. Coppel and M.P. Manns. Aetiology
of primary biliary cirrhosis. In: Falk Symposium 135: Immunological Diseases of Liver and Gut. Lukas,
M., M.P. Manns, J. Spicak, and E.F. Strange, eds., pp. 78-91. Kluwer Academic Publishers, The
Netherlands.
Leung, P.S.C., Manns, M.P., Coppel, R.L. and Gershwin, M.E. 2002. Detection of antimitochondrial
Autoantibodies in primary biliary cirrhosis and LKM antibodies in autoimmune hepatitis. In Manual of
Clinical Laboratory Immunology 6th ed, pp. 1023-31. Rose N. R, Hamilton, R.G. and Detick, B.. (ed).
ASM Press, Washington D.C.
Leung, P.S.C., Obermayer-Strab, P., Manns, M.P., Ansari, A, and Gershwin, M.E. 2001.
Inflammatory Hepatobiliary Cirrhosis. In Clinical Immunology: Principles and Practice. Vol. II. Rich R.R.
(ed). pp.88.1-88.14. Mosby-Year Book, Inc., St. Louis, Missouri.
Tanaka, A., P.S.C. Leung, J. Van de Water, S. Munoz, R.L. Coppel, A. Ansari and M.E. Gershwin.
2000. Clues to the aetiology of primary biliary cirrhosis. In: Immunology and Liver. M.P. Manns, G.
Paumgartner, and U. Leuschner, eds., pp. 244-256. Kluwer Academic Publishers, The Netherlands.
Leung, P.S.C., Manns, M.P., Coppel, R.L. and Gershwin, M.E. 1997. Detection of antimitochondrial
Autoantibodies in primary biliary cirrhosis and LKM antibodies in autoimmune hepatitis. In Manual of
Clinical Laboratory Immunology, pp. 989-996. Rose N. R. (ed). ASM Press, Washington D.C.
Leung, P.S.C. and M.E. Gershwin. Ig genes and idiotype of anti-mitochondrial antibodies in
primary biliary cirrhosis. 1997. In: Idiotypes in Medicine: Autoimmunity, Infection and Cancer, Eds. Y.
Shoenfeld, R.C. Kennedy, S. Ferrone, Elsevier Science, pp. 113-118.
Leung, P.S.C., Coppel, R.L., and Gershwin, M.E. 1996. Mitochondrial autoantibodies. In:
Autoantibodies. Peter, J.B., and Shoenfeld, Y. (eds.), Elsevier Science Publishers, pp. 494-500.
Galperin, C., Leung, P.S.C. and M. Eric Gershwin. 1996. Molecular Biology of Autoantigens in
Rheumatic Diseases. In Rheumatology Disease Clinics of North America. Vol. 22. No.1 Calabrese L.H.
(ed). W.B. Saunders Company.
Leung, P.S.C., Manns M.P. and Gershwin, ME. 1995. Inflammatory hepatobiliary cirrhosis. In
Clinical Immunology: Principles and Practice. Vol. II. pp.1429-1443. Rich R.R. (ed). Mosby-Year Book,
Inc., St. Louis, Missouri.
Leung, P.S.C., Mackay I. and Gershwin, M.E. 1994. Mitochondrial Autoantigens. In Manual of
Biological Markers of Disease Vol. II. van Venrooji W.J. and Maini R.N. (eds)., B8.1:1-14 Kluwer
Academic Publishers, The Netherlands.
Gershwin, M.E., Van de Water, J., Leung, P.S.C., and Coppel, R. T cell biology and cell surface
expression of PDC-E2 in primary biliary cirrhosis. In: Immunology and Liver, pp. 373-382. Johnstone, P.
(ed). Kluwer Academic Publishers.
Gershwin, M.E., Rowley, M., Davis, P.A., Leung, P., Coppel, R. and Mackay, I.R. 1992. Molecular
biology of the 2-oxo-acid dehydrogenase complexes and anti-mitochondrial antibodies. In: Progress in
Liver Diseases. pp.47-61.
Abstracts/Presentations:
11
Leung, P.S.C., Naiyanetr, P., Butler, J.D., Meng, L., Kurth, M., Lam, K., Ansari, A., Coppel, R.L.
and Gershwin M.E. Modification of the lipoyl moiety of PDC-E2 by electrophilic agents elicits
antimitochondrial autoantibodies in primary biliary cirrhosis. Presented in the International Congress of
Autoimmunity 2010.
Naiyanetr, P., Meng, L., Butler, J.D., Pfeiff, J., Kenny, T.P., Lam, K.S., Kurth, M.J., Leung, P.S.C.,
and Gershwin, M.E. Environmental causes of autoimmunity: Clues to the loss of tolerance in primary
biliary cirrhosis. Presented in the annual meeting of the American Association of Immunologists, 2009.
Lee, Y.S. Leung, P.S.C., Fung, M.C. and Chu. K.H. Humoral and T cell responses in a mouse
model of shrimp allergy. XXVI Congress of the European Academy of Allergology and Clinical
Immunology 2007.
Reiger, R., Leung, P.S.C., Jeddeloh, M.R., Kurth, M., Nantz, M.H., Lam, K., Barsky, D., Ansari,
A., Coppel, R., Mackay, I.R. and Gershwin, M.E. Identification of a common occupational chemical
derivative with high primary biliary cirrhosis AMA reactivity. Presented in the 57 th AASLD meeting 2006.
Park, O., Leung, P.S.C., Davis, P.A.. Lee, W.M., Miyakawa, H., Rossaro, L., Han, S., Davern, T.
and Gershwin, M.E. Identificaion of antimitochondrial autoantibodies (AMA) in acute liver patient sera:
support for hepatic damage as a precursor for primary biliary cirrhosis. Presented in the 56th AASLD
meeting 2005.
Leung, P.S.C., Park, O., Selmi, C., Ansari, A., Coppel, R.L., Gershwin, M.E. Analysis of specific
inhibition of Novosphingobium aromaticivorans pyruvate dehydrogenase enzyme function by clinically
defined and staged PBC sera. Presented in the 56th AASLD meeting 2005.
Reiger, R., Leung, P.S.C., Suen, Y.F., Kurth, M., Lam, K., Ansari, A., Coppel, R., and Gershwin,
M.E. Chemical xenobiotic reactivity in primary biliary cirrhosis: QSAR analysis of alkynoic compounds.
Presented in the 56th AASLD meeting 2005.
Norman, G.L., Lee, L., Shums, Z., Bogdanos, D-P., Worman, H.J., Krawitt, E.D., Leung, P., Aoki,
C., Verganim D. and Gershwin, M.E. Enhanced detection and characterization of primary biliary cirrhosis
patients with new M2 EP(MIT3), gp210 and sp100 ELISA assays. Presented at the annual meeting of
Association of Medical Laboratory Immunologist, 2005.
Amano, K., Leung, P.S.C., Quan, C., Mraik, J., Suen, Y.F., Kurth, M., Nantz, M., Ansari, A., Lam,
K., Zeniya, M., Coppel, R.L. and Gershwin, M.E. Quantitative structural relationship analysis of chemical
xenobiotics and mitochondrial autoantigens in primary biliary cirrhosis: is PBC caused by a chemical
exposure? Presented at the 55th AASLD meeting. 2004.
Kimura, Y., Selmi, C., Leung, P.S.C., Mao, T.K., Warnik, M., Kuriyama, S., Nishioka, M., Rousai,
E., Ansari, A., Coppel, R.L., Invernizzi, P., Podda, M., Gershwin, M.E. Genetic variants influencing
xenobiotic metabolism in patients with primary bilairy cirrhosis. Presented at the 55 th AASLD meeting.
2004.
Kikuchi, K., Lian, Z.X., Leung, P.S.C., Yang, G.X., Coppel, R.L., Ansari, A., Ikehara, S.,
Miyakawa, H., Kaplan, M. and Gershwin, M.E. The elevated serum IgM in parimary biliary cirrhosis is
secondary to abnormal responses to selected bacterial CpG motifs. Presented at the 55th AASLD
meeting. 2004.
Tang C.Y., Leung P.S.C., Chiang B.L., Fung M.C., and Chu K.H. Induction of shrimp
tropomyosin specific IgE and T cell response in mice. Presented in the European Allergy and Clinical
Immunology Congress XXIII 2004. Amsterdam.
Leung, P.S.C., Matsumura, S., Park, O., Van de Water, J., Quan, C., Kurth, M., Nantz, M.,
Ansari, A., Coppel, R. and Gershwin, M.E. Induction of primary biliary cirrhosis in guinea pigs following
immunization with a chemical xenobiotic. Presented at the annual 54thAASLD meeting, 2003.
Selmi, C., Balkwill, D.L., Invernizzi, P., Ansari, A., Coppel, R.L., Podda, M., Leung, P.S., Kenny,
T.P., Van de Water, J., Schauer, J., Nantz, M.H., Kurth, M.J. and Gershwin, M.E. Is primary biliary
cirrhosis caused by molecular mimicry with Novosphingobium aromaticivorans, a ubiquitous xenobiotic
metabolizing bacterium. Presented at the annual 54th AASLD meeting, 2003.
Amano, K., Leung, P.S.C., Xu, Q., Marik, J., Quan, C., Kurth, M., Nantz, M., Ansari, A., Lam, K.,
Coppel, R., Zeniya, M. and Gershwin, M.E.. Use of a conjugated peptide microarray for ultra fine
mapping of antimitochondrial antibodies. Presented at the annual 54thAASLD meeting, 2003.
Matsumura, S, Leung, P.S.C., Van de Water,J., Yamamoto, K., Shiratori, Y.,, Gores, G.J.,
Ansari, A.A., Coppel, R.L., and Gershwin, M.E. Antimitochondrial IgA antibody induces apoptosis to
MDCK cells bearing human polymeric immunoglobulin receptor. Presented at the annual 54 thAASLD
meeting, 2003.
Tang, C.Y., Li, C.P., Fung, M.C., Chiang, B.L., Leung, P.S.C. and Chu, K.H. Expression of the
major shrimp allergen tropomyosin. Presented in the Croucher Advanced Study Institute – Advances in
Protein Sciences, Dec. 15-20, Hong Kong, 2003.
12
Wu, A. , Chik, S.C., Williams, G.A. and Leung P.S.C.. 2003 Cross-reactivity between different
species of gastropod mollusks. Presented in European Academy of Allergology and Clinical Immunology
Annual Meeting, 2003.
Leung, P.S.C., Quan, C., Park, O., Van de Water, J., Kruth, M., Nantz, M., Ansarim A., Coppel,
R.L. and Gershwin, M.E. Immunization with a xenobiotic breaks tolerance to mitochondrial autoantigens.
53rd Annual AASLD meeting. 2002.
Leung, P.S.C., Park, O. and Gershwin, M.E. Is there a relation between Chlamydia infection and
primary biliary cirrhosis ?, 53rd Annual AASLD meeting 2002.
Kimura, Y., Leung. P.S.C., Kenny, T.P., Van de Water, J., Nishioka, M., Giraud, A.S., Neuberger,
J., and Gershwin, M.E. Differential Expression of Intestinal trefoil factor (ITF) in biliary epithelial cells of
primary biliary cirrhosis. DDW meeting. 2002.
Leung, P.S.C. and Chu, K.H. cDNA cloning and molecular identification of the major oyster
allergen from the Pacific oyster Crassostrea gigas. International Marine Biotechnology Conference,
2000.
Tanaka, A., Reynoso-Paz, S., Leung, P.S.C., Van de Water, J., Munoz, S., Bass, N.,
Lindor,
K., Donald, P.J., Coppel, R.L., Ansari, A.A. and Gershwin, M.E. The presence of mitochondrial
antibodies in saliva in primary biliary cirrhosis-evidence for a locally driven mucosal response. DDW
meeting, 2000.
Leung, P.S.C., Wong, S.H. and Chu, K.H. Expression of tropomyosin as mollusk allergens in
abalone, scallop and mussel. The 56th Annual Meeting of the American Academy of Allergy and
Immunology. 2000.
Wong, S.H., Leung, P.S.C. and Chu, K.H. RT-PCR amplification and expression of tropomyosin,
the major shellfish allergen from abalone, scallop and mussel. The 8th Symposium of Chinese
Bioscientists in America. 1999.
Dubel, L., Tanaka, A., Leung, P.S.C., Van de Water, J., Roche, T., Coppel, R and Gershwin, M.E.
Cloning and Autoepitope analysis of autoantibodies at the E3 binding protein (Protein X) in patients with
primary biliary cirrhosis. The 49th Annual Meeting of the American Association for the Study of Liver
Diseases, Nov.1998.
Migliaccio, C., Nishio, A., Van de Water, J., Ansari, A. A., Leung, P.S.C., Nakanuma, Y., Coppel,
R.L. and Gershwin, M.E. Monoclonal antibodies to mitochondrial E2 components define autoepitopes of
a molecular mimic in primary biliary cirrhosis. The 49th Annual Meeting of the American Association for
the Study of Liver Diseases, Nov.1998.
Quaranta, S., Shulman, H., Paganin, S., Shoenfeld, Y., Ahmed, A., Peter, J., Leung, P.S.C., Van
de Water, J., Coppel, R., Ostlund, C., Worman, H., Rizzetto, M., Tsuneyama, K., McDonald, G.B.,
Rosina, F. and Gershwin, M.E. Is GVDH a model of PBC? Characterization of autoantibodies in GVDH.
The 49th Annual Meeting of the American Association for the Study of Liver Diseases, Nov.1998.
Van de Water, J., Malmborg, A-C., Shultz, D.B., Luton, F., Mostov, K.E., Richly, E., Leung,
P.S.C., Benson, G.B., Ansari, A.A., Coppel, R.L. and Gershwin, M.E. Penetration and co-localization in
MDCK cells mitochondria of IgA derived from patients with primary biliary cirrhosis. The 49th Annual
Meeting of the American Association for the Study of Liver Diseases, Nov.1998.
Mattalia, A., Leung, P.S.C., Bauducci, M., Van de Water, J., Quaranta, S., Calvo, P.L., Danielle,
F., Ansari, A., Rizzetto, M., Bonardi, L., Rosina, F., and Gershwin, M.E.. Prevalence and significance of
antimitochondrial antibodies detected by recombinant autoantigens in “healthy” adults. The 48th Annual
Meeting of the American Association for the Study of Liver Diseases, 1997.
Leung, P.S.C. and K.H. Chu. Molecular and Immunological Studies on shellfish allergens.
Presented at the Fourth International Marine Biotechnology Conference 1997.
Chu, K.H., Chow, W.K. and Leung, P.S.C. Tropomyosin as the common cross-reactive allergen.
Presented at the Asia-Pacific Conference on Science and Management of Coastal Environment, Hong
Kong, 1996.
Leung, P.S.C., Cha, S., Coppel, R.L., Van de Water, J., Schultz, P.G. and Gershwin, M.E.
Random phage mimotopes recognized by monoclonal antibodies to PDC-E2. The 9th International
Congress of Immunology, 1996
Leung, P.S.C. Antimitochondrial autoantibodies in primary biliary cirrhosis. The Asian Pacific
Congress of Allergology and Clinical Immunology. 1995, Taipei, Taiwan.
Galperin, C., Leung, P.S.C., Ansari, A., Coppel, R., van Thiel, D., Fiocchi, C., Dickson, E.R. and
Gershwin, M. E. Specific antibodies to Mycobacteria in primary biliary cirrhosis. 1995 Annual meeting of
the American Society for Microbiology.
Halpern GM and Leung P.S.C. Evaluation of possible histamine release from human basophils
using an EIA(HRT) with components of intravenous catheters. Presented at the XV International
Congress of Allergology and Clinical Immunology, 1994.
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Chu KH, Chow WK, Kwan HS, Nagy SM, Gershwin, ME and Leung P.S.C. Cloning, expression
and sequencing of the major heat stable allergen from the shrimp Metapenaeus ensis. Third International
Marine Biotechnology Conference. 1994.
Cha, S., Leung, P.S.C., Ansari, A. and Gershwin, M.E. Development of combinatorial
autoantibodies to define the autoantibody repertoire in primary biliary cirrhosis. American Association for
the Study of Liver Disease. Nov. 1993.
Leung, P.S.C., Cha, S., Gershwin, M.E., Fletcher, M.P., Ansari, A.A. and Coppel, R.L.
Combinatorial autoantibodies to dihydrolipoamide acetyltransferase, the major autoantigen of primary
biliary cirrhosis. 1993 AAI meeting.
Halpern, G. and Leung, P. Evaluation of nickel release from metallic prostheses using the
dimethylglyoxine test. Societe Francaise d'Allergologie et Immunologie Clinique, Institut Pasteur. Jan
1993.
Leung, P.S.C., Wynn, R. M., Cha, S., Chuang, D.T., Ansari, A. and Gershwin, M.E. Epitope
mapping of BCOADC-E2 in primary biliary cirrhosis. 1992 FASEB meeting.
Leung P.S.C., Kenny, T., Haapanen, L., Krams, S., Robbins, D.L., Fung, J., Starzl, T.E., Coppel,
R. and Gershwin, M.E. Human hybridomas specific for PDC-E2, the major mitochondrial autoantigen of
PBC. 1991 FASEB meeting.
Iwayama, T., Leung P.S.C., Roche, T.E., Patel, M., Mizushima, Y., Coppel, R.L. and Gershwin,
M.E. Specific reactivity of an expression clone of pyruvate dehydrogenase E1 alpha in primary biliary
cirrhosis. 1991 FASEB meeting.
Leung, P., Surh, C.D., Coppel, R.L., Ahmed, A. and Gershwin, M.E. The predominance of IgG3
and IgM isotype antimitochondrial autoantibodies against recombinant fused mitochondrial polypeptide in
patients with primary biliary cirrhosis. 1988 FASEB meeting.
Van de Water, Coppel, R., Leung, P., Ahmed, A. and Gershwin, M.E. Identification of the
immunodominant mitochondrial epitope recognized by autoantibodies in primary biliary cirrhosis. 1988
FASEB meeting.
Gershwin, M.E., Ahmed. A., Danner, D., Fregeau, D., Van de Water, J., Leung, P. and Coppel, R.
Identification of the mitochondrial enzymes that react with autoantibodies from patients with primary
biliary cirrhosis (PBC). 1988 FASEB meeting.
Leung, P., Gershwin, M. E., Halpern, G. and Takeuchi, P. Western blotting of Aspergillus
fumigatus. 1987 FASEB meeting.
Leung, P. and Preiss, J. Nucleotide sequence of Salmonella typhimurium LT2 glgC gene. 1986
Annual meeting of the American Society for Microbiology.
Leung, P. and Preiss, J. Cloning of the ADPglucose pyrophosphorylase glgC and glycogen
synthase glgA genes of Salmonella typhimurium LT2. 1985 Annual meeting of the American Society for
Microbiology.
Esch, K., Leung, P., Boylan, S., Greenberg, E. and Preiss, J. Cloning of the Escherichia coli K12
allosteric mutant ADPglucose synthetase structural gene. 1985 Annual Meeting of the American Society
for Microbiology.
Lee, Y.M., Leung, P., Greenberg, E., Boylan, S., Esch, K. and Preiss, J. Cloning and expression
of an Escherichia coli K12 mutant glgC gene. Presented at the 1985 FASEB.
Larsen, C.E., Lee, Y.M., Leung, P. and Preiss, J. Subcloning, expression and sequence
determination of the Escherichia coli glgA gene. 1985 FASEB.
Baeker, P.A., Urbanowski, J., Leung, P., Weissbach, H. and Preiss, J. The nucleotide sequence
of the glgC gene and stimulation of the in vitro synthesis of the glgC product. 1983 Annual Meeting of the
American Society for Microbiology.
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