Hemoglobin
Sample A
57.9% mole (33.6% wt) of glycerol:
8.3 g of glycerol plus 16.4 g of water
Viscosity:
ln() = -2.32 + 17.49x107 / T3

Viscosity in centipoise
Correlation time calibration
r = 7.4x10-4  (in poise) / T
Sample B
9% mole (33.6% wt) of glycerol:
8.3 g of glycerol plus 16.4 g of water
Viscosity:
ln() = -2.3115 + 8.58x107 / T3
Note: it freezes at -29 C
Preparation from the Hemglobin concentrations
Notes to keep in mind:
1.
water in your final mixture comes from the Hb solution. The content of water
in the Hb solution is inversely proportional to the concentration. Therefore,
how much Hb solution you can add to glycerol is a function of its
concentration.
2.
glycerol is difficult to pipette or weigh out, Hb is easier, therefore weigh out
amount of pure glycerol (from the bottle labeled for the ST-EPR only, dried of
molecular sieve and taped with Parafilm) first. Knowing how much glcerol you
have, determine how much Hb solution you should add.
3.
When calculating the amount of Hb solution take into account the
displacement of water by Hb. Calculate the volume occupied by Hb
molecules from its concentration and from partial specific volume of a protein
(Cantor & Shimmel or van Holde 0.73 (? Check) mls/g (???) - SEE
CALCULATIONS IN THE FOLDER.
4.
remember that even smallest deviation of the water content changes the
viscosity dependence on temperature – i.e. you cannot predict what the
correlation time is.