Genetics of Organisms: Lab Quiz Answers You have been given a vial containing the fruit flies shown below. After two weeks, you collect the offspring from this pair and obtain the results shown in the following table. Use this table to answer Questions 1 – 5. 1. On the basis of the results shown in the table, which statement is most likely true? a. The genes for red eyes and normal wings are linked. b. The gene for no wings is sex-linked. c. The gene for red eyes and the gene for no wings are both dominant. d. The gene for eye color is inherited independently of the gene for wings. e. The F1 mates were both homozygous for both eye color and wings. The correct response is d. There is no evidence for any type of linkage, since both males and females show the traits in approximately equal proportions, and eye color and wings appear to sort independently. If the parents were homozygous for these traits, the offspring would not show different phenotypes from both parents. 2. If the parents were both heterozygous for eye color and wings, what phenotypic ratio would you predict for the offspring if these traits are not linked? a. 1 red eyes/no wings 1 red eyes/normal wings 1 sepia eyes/normal wings 1 sepia eyes/no wings b. 9 red eyes/normal wings 3 red eyes/no wings 3 sepia eyes/normal wings 1 sepia eyes/no wings c. 3 red eyes/normal wings 1 sepia eyes/no wings d. 1 red eyes/normal wings 2 sepia eyes/normal wings 1 red eyes/no wings 2 sepia eyes/no wings The correct response is b. If there were linkage, choice a would be correct, but when two traits sort independently, the offspring of heterozygous parents will show the traits in a 9:3:3:1 ratio. 3. Based on the hypothesis that this is a dihybrid cross, with the two genes unlinked, calculate using the data in the table of observed phenotypes. a. b. c. d. 6.04 7.81 4.977 24.0 The correct answer is c. This is obtained by using the formula = the sum of Red eyes/normal wings Red eyes/no wings Sepia eyes/normal wings o e o-e 98 90 8 0.7111 22 30 8 2.1333 26 30 4 0.5333 Sepia eyes/no 14 wings 10 4 1.6 = 4.977 4. How many degrees of freedom are there for this data? a. b. c. d. 1 2 3 4 The correct answer is c. There are 4 categories minus 1 = 3 degrees of freedom. 5. Compare the chi-square value obtained above with the Critical Values Table below for p = 0.5. Which of the following statements would be true? Degrees of freedom (df) 1 2 3 0.05 3.84 5.99 7.82 0.01 6.64 9.21 11.3 Probability (p) 0.001 10.8 13.8 16.3 4 5 9.49 11.1 13.2 15.1 18.5 20.5 a. Since the calculated value for chi-square is less than 7.815, the results support the hypothesis that the parents are heterozygous for two unlinked traits. b. Since the calculated value for chi-square is less than 7.815, the results support the hypothesis that eye color and wings are linked. c. Since the calculated value for chi-square is less than 7.815, the results are inconclusive. The experiment should be repeated. d. Since the calculated value for chi-square is so large, the variations from the expected are excessive. The experiment should be repeated. The correct response is a. The expected results are based on obtaining a 9:3:3:1 ratio from two heterozygous parents. Since the chi-square value is below 7.815, the results are statistically significant.