UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet.....1...... of....4........
Title of Experiment
Room/Laboratory
Site
1.
Colourimetric DNA Estimation
23 Nov 2007
Lab 6.8
Brief description of work
Colourimetric estimation of unknown DNA solution using a DNA standard Curve
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually required.
Note also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)
Manufacturers Safety Data
Computer Data Base
Product Label
Other (Specify)
NAME OF SUBSTANCE
(CODE)
PHYSICAL
DESCRIPTION
MAX.
QUANTITY
EXPOSED TO
Sulphuric Acid
liquid
5l
Acetic Acid
Liquid
5l
Diphenylamine
solid
100 g
DNA
Fibrous
solid
1g
DATA AVAILABLE
(YES/NO)
Y
KNOWN HAZARDS
(See Note;
use numbers)
5,6, 7, 12, 13,
14, 15,
Y
3,4,5,6,7,9,12,1
3, 14, 15,
Y
4, 5, 7, 8, 10,
11, 12, 13,14,
15,
Y
Safety info. not
available
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
3.
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
4.
Ref Note 17, 18: see EH40 (current edition).
INITS
STATUS
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
4BLY413
All students
& staff
occasionally
Vanita Amin
Lab
Technical
Support
occasionally
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Storekeepers
State control measures (to prevent inappropriate persons/groups having access to the substances ):
The reagents are only available to the designated students at their appropriate, scheduled
practical time
5.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify bel ow action to be
taken.
CODE
Acetic acid
SPILLAGE/UNCONTROLLED RELEASE
Evacuate area.
Wear self-contained breathing
apparatus, rubber boots, and
heavy
rubber gloves.
METHODS FOR CLEANING UP
Cover with dry lime or soda ash,
pick up, keep in a closed
container, and hold for waste
disposal. Ventilate area and wash
spill site after material pickup is
complete.
FIRE
EXTINGUISHING MEDIA
Suitable: Carbon dioxide, dry chemical
powder, or appropriate
foam.
SPECIAL RISKS
Specific Hazard(s): Combustible liquid. Emits
toxic fumes under
fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR
FIREFIGHTERS
Wear self-contained breathing apparatus and
protective clothing
to prevent contact with skin and eyes.
Sulphuric
acid
Wear respirator, chemical safety
goggles, rubber boots, and
heavy rubber gloves.
METHODS FOR CLEANING UP
Cover with dry lime or soda ash,
pick up, keep in a closed
container, and hold for waste
disposal. Ventilate area and wash
spill site after material pickup is
complete.
May react with metals, releasing flammable
hydrogen gas.
EXTINGUISHING MEDIA
Suitable: Noncombustible. Use extinguishing
media appropriate to surrounding fire
conditions.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under
fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR
FIREFIGHTERS
Wear self-contained breathing apparatus and
protective clothing to prevent contact with skin
and eyes.
Diphenylamine
Evacuate area.
PROCEDURE(S) OF
PERSONAL RECAUTION(S)
Wear self-contained breathing
apparatus, rubber boots, and
heavy rubber gloves.
METHODS FOR CLEANING UP
Sweep up, place in a bag and
hold for waste disposal. Avoid
raising dust. Ventilate area and
wash spill site after material
pickup is complete.
EXPLOSION DATA
Dust Potential: This material, like most
materials in powder form, is capable of
creating a dust explosion.
EXTINGUISHING MEDIA
Suitable: Carbon dioxide, dry chemical
powder, or appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes
under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT
FOR FIREFIGHTERS
Wear self-contained breathing
apparatus and protective clothing
to prevent contact with skin and eyes.
DNA
Not available
Not available
6. CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other necessary
measures. SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
1. Limiting exposure time of personnel - state maximum time
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
Acetic Acid First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give
oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove
contaminated clothing and shoes.
Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure
adequate flushing by separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is conscious. Call a physician. Do not
induce vomiting.
Sulphuric Acid First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give
oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove
contaminated clothing and shoes. Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure
adequate flushing by separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is conscious. Call a physician. Do not
induce vomiting.
Diphenylamine First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give
oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove
contaminated clothing and shoes. Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure
adequate flushing by separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is conscious. Call a physician
immediately.
(NB. Antidotes and special treatment may be obtained through...................................................................................................................................)
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.) SPECIFY
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
For all reagents:
Exposure time is limited to a maximum of 2hours, with good laboratory ventilation. In addition,
heating of solutions for 10 minutes is done in a water bath, in a fume cupboard, with covered
tubes.
Gloves and Safety goggles, and pipette fillers are provided.
STORAGE - SAFETY CONSIDERATIONS
Acetic Acid
Keep tightly closed. Keep away from heat
and open flame.
SPECIAL REQUIREMENTS: Moisture
sensitive.
HANDLING PRECAUTIONS
Acetic Acid
Directions for Safe Handling: Do not breathe
vapor. Do not get in eyes, on skin, on clothing.
Avoid prolonged or repeated exposure.
Diphenylamine
Conditions of Storage: Keep tightly closed.
Diphenylamine
Toxic by inhalation, in contact with skin and if
swallowed. Danger of cumulative effects. Very
toxic to aquatic organisms, may cause
long-term adverse effects in the aquatic
environment.
Directions for Safe Handling: Do not breathe
dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or
repeated exposure.
Sulphuric Acid
Conditions of Storage: Keep tightly closed.
Sulphuric Acid
Directions for Safe Handling: Do not breathe
vapor. Do not get in eyes, on skin, on clothing.
Avoid prolonged or repeated exposure.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Any remaining DNA solutions to be flushed down the sink with running water. Other unused
reagents eg colourimetric solutions to be placed in clearly –labelled DISCHE WASTE bottles which
are place in the fume-cupboard. These will be collected for disposal.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have
brought the assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Dr Geoff Stubbs
Signed:
Status of Assessor:
Senior Academic
Date: Nov 2007