5th Annual REU-RET Research Day
2014
Symposium Program
2014 5th USF NSF REU-RET Day
Symposium Program
Interdisciplinary Research Building Galleria (IDRB)
Thursday, July 31, 2014
USF Research Park
Agenda
10:00 AM-11:00 AM
Poster Set-Up
11:00 AM-11:15 AM
Welcoming Remarks
Paul Sanberg, Ph.D., D.Sc. Senior Vice President for
Research & Innovation, Distinguished University Professor,
University of South Florida
Rafael Perez, Ph.D., Professor, Interim Dean, College of
Engineering, University of South Florida
11:15 AM-1:15 PM
Poster Viewing
NOON-1:00 PM
Lunch
1:15 PM-1:45 PM
Closing Remarks and Certificate Awards
Robert Potter, Ph.D., Professor, Associate Dean, College of
Arts and Sciences, University of South Florida
Larry Plank, Ed.S. Director of K-12 STEM Education,
Hillsborough County Public Schools
Awards presented by REU & RET Programs
1:45 PM-2:30 PM
Group Photos
Poster Breakdown
Sponsors
NSF RET: Functional Materials Research Institute (FMRI)
In Service Teacher
Dr. Megan Faliero
Sandra Gebhard
Andria Keene
Artemio Perez
Christina Rutledge
Steven Sanden
Pedro Villavicencio
Erica Wilkes
Dr. Carol Zavarella
Pre Service Teacher
Jordan Lewis
Institution
Durant High School
Brandon High School
Strawberry Crest High School
Plant City High School
Strawberry Crest High School
Middleton High School
Steinbrenner High School and PHSC
C. Leon King High School
Hillsborough Community College
Institution
University of South Florida
PI: Professor Venkat Bhethanabotla, Chemical & Biomedical Engineering
Co-PI: Professor Sylvia Thomas, Electrical Engineering
Coordinator: Professor Scott Campbell, Chemical & Biomedical Engineering
Faculty Research Mentors
Dr. Scott Campbell, Chemical & Biomedical Engineering
Dr. Nathan B. Crane, Mechanical Engineering
Dr. Nathan Gallant, Mechanical Engineering
Dr. Piyush Koria, Chemical & Biomedical Engineering
Dr. John Kuhn, Chemical & Biomedical Engineering
Dr. Shengqian Ma, Chemistry
Dr. Anna Payat, Chemical & Biomedical Engineering
Dr. Brian Space, Chemistry
Dr. Sylvia W. Thomas, Electrical Engineering
Dr. Ryan Toomey, Chemical & Biomedical Engineering
Graduate Student Mentors
Olukemi Akintewe, Chemical & Biomedical Engineering
Edikan Archibong, Chemical & Biomedical Engineering
Brian Bell, Mechanical Engineering
Christian R. Cioce, Chemistry
Ana Stringari de Castro, Chemical & Biomedical Engineering
Hruday Katakam, Electrical Engineering
Dagmara Monfort, Chemical & Biomedical Engineering
Rasudha Muppaneni, Electrical Engineering
Qi Ni, Mechanical Engineering
Samuel Perez, Electrical Engineering
Jon Pickering, Chemical & Biomedical Engineering
Karthik Raj, Chemical & Biomedical Engineering
Manopriya Subramanyam, Electrical Engineering
Kia Williams, Chemistry
Brant Tudor, Chemistry
NSF RET in Engineering and Computer Science Water Awareness
Research and Education (WARE)
In Service Teacher
Courtney Taylor
Frank Marcantoni
Ileana Bermudez Luna
Joseph McLaughlin
Ken Wood
Kristy Carew
Lindsay Guntner
Maranda Holley
Matthew Liebelt
Mirko Soko
Nicole Richards
Stephanie Quintero
Tana Jackson
Taryn Kendig
Pre Service Teacher
Ete Hires
Matthew Sestilio
Student
Andres Garcia
Institution
Learning Gate Community School
Van Buren Middle School
Freedom High School
Howard W. Blake High School
Wharton High School
King Senior High School
Paul R. Wharton High School
Adams Middle School
Plant City High School
Adams Middle School
C. Leon King High School
Steinbrenner High School
Tampa Bay Technical High School
Van Buren Middle School
Institution
University of South Florida
University of South Florida
Institution
Paul R. Wharton High School
PI: Maya Trotz, Ph.D.
Associate Professor, Department of Civil and Environmental Engineering
Co-PI: Yiping Lou, Ph.D.
Associate Professor, Instructional Technology, College of Education
Graduate Coordinator: Ryan Locicero
Faculty Mentors
Dr. Daniel Yeh, Civil and Environmental Engineering
Dr. Maya Trotz, Civil and Environmental Engineering
Dr. Sarina Ergas, Civil and Environmental Engineering
Graduate and Post-Doctoral Mentors
Adib Amini, Civil and Environmental Engineering
George Dick, College of Engineering
Jorge Calabria, Civil and Environmental Engineering
Laura Rodriguez-Gonzalez, Civil and Environmental Engineering
Maureen Kinyua, Civil and Environmental Engineering
Dr. Meng Wang, Civil and Environmental Engineering
Ryan Locicero, Civil and Environmental Engineering
Suzanne Boxman, Civil and Environmental Engineering
Veronica Apontes-Morales, Civil and Environmental Engineering
NSF REU in Applied Physics (AP)
REU Student
Izabella Berman
Janae Bradley
Jonathan Cuthbertson
Brad Davis
Samuel Hebenstreit
Major
Physics
Biological Engineering
Physics
Mechanical/Aerospace Engineering
Physics
Luis Martinez Milian
Physics
Carl McIntyre
Boeing Smith
Physics
Physics
Kimberly Sonju
Applied Physics
Otho Ulrich
Physics
University or College
Beloit College
University of Missouri
Wheaton College
Bronx Community College
Colorado Mesa University
University of Puerto Rico at
Humacao
Colorado Mesa University
Wittenberg University
California State University, San
Bernadino
Western Michigan University
PI: Sarath Witanachchi, Ph.D.
Professor, Associate Chair, Department of Physics
Graduate Coordinators: Kristen Stojak Repa, Domingo Mateo Feliciano, Daniel Denmark
Faculty Advisers
Dr. Anuja Datta, Physics
Dr. Denis Karaiskaj, Physics
Dr. Garrett Matthews, Physics
Dr. Ivan Oleynik, Physics
Dr. Jianjun Pan, Physics
Dr. Sagar Pandit, Physics
Dr. Manh-Huong Phan, Physics
Dr. Hariharan Srikanth, Physics
Dr. Jiangfeng Zhou, Physics
Dr. Sarath Witanachchi, Physics
Graduate Mentors
Michael Cross, Physics
Daniel Denmark, Physics
Jagganath Devkota, Physics
Prasenjit Dey, Physics
Joseph Fogarty, Physics
Joseph Gonzalez , Physics
Nawal Khadka, Physics
Corisa Kons, Physics
Zohreh Nemati, Physics
Jagganath Paul, Physics
Kristen Stojak Repa, Physics
Rishi Silva, Physics
NSF REU: Chemistry Summer Enhanced Experience Discovering Science
(Chem-SEEDS)
Participant Name
Christopher Brewer
Veronika Espinoza
Arielis Estevez
Nelson Garcia-Vazquez
Jermaine Henderson
Reed Jacobsen
Josue Liriano
Nathan Pickering
Santiago RodriguezOspina
Vinushka Schalk
Anne-Genevieve Telcy
Major
Chemistry & Biochemistry
Chemistry
Chemistry
Chemistry
Chemistry
Biochemistry
Biomedical Sciences
Chemistry
Microbiology &
biochemistry
Chemistry/Computer
Science
Chemistry
PI: Santiago Sandi-Urena, Ph.D.
Assistant Professor, Department of Chemistry
Graduate Coordinator: Parker Huhta
Faculty Advisers
Dr. Jon C. Antilla, Chemistry
Dr. Kirpal Bisht, Chemistry
Dr. Julie Harmon, Chemistry
Dr. Sheryl Li, Chemistry
Dr. Abdul Malik, Chemistry
Dr. David J. Merkler, Chemistry
Dr. Arjan van der Vaart, Chemistry
Dr. H. Lee Woodcock, III, Chemistry
Dr. Peter Zhang, Chemistry
Graduate & Post-Doc Mentors
Susana S. Lopez, Chemistry
Ali Husain, Chemistry
Tamalia Julien, Chemistry
Sung Yub Han, Chemistry
Sheshanka Kesani, Chemistry
Daniel R. Dempsey, Chemistry
Geoffrey M. Gray, Chemistry
Yuri Pevzner, Chemistry
Dr. Li-mei Jin, Chemistry
University or College
Florida State University
California State University-San Marcos
University of Puerto Rico-Rio Piedras
University of Puerto Rico-Rio Piedras
University of Massachusetts-Boston
University of South Florida
University of South Florida
Northwest Missouri State University
University of South Florida
New College of Florida
Florida Southern College
NSF REU in Ubiquitous Sensing
Participant Name
Alejandra Vidal Ortiz
Edwin Miguel Peguero
Luis N. Valcourt
Mark Di Sano
Steven Díaz Hernández
Major
Computer Engineering
Mathematics and Computer
Science
Computer Science
Computer Engineering
Computer Engineering
University or College
Universidad del Turabo
University of South Florida
University of Puerto Rico at Bayamon
University of South Florida
Universidad del Turabo
PI: Miguel Labrador, Ph.D.
Professor, Department of Computer Science and Engineering
Graduate Coordinator: Idalides Vergara
Faculty Mentors
Dr. Miguel Labrador, Computer Science and Engineering
Dr. Sudeep Sarkar, Computer Science and Engineering
Graduate and Post-Graduate Mentors
Yueng De La Hoz, Computer Science and Engineering
Juan José Marrón, Computer Science and Engineering
Ravichandra Subramanian, Computer Science and Engineering
NSF REU: Tampa Interdisciplinary Environmental Research (TIER)
Participant Name
Allison Wood
Ariane Ramsundar
Major/ Program
Environmental Engineering
Applied Mathematics
Chinelo Agbim
Civil & Environmental Engineering
Christopher Roberts
Civil Engineering
Clyde Joseph
Cove Capodice
Donald Dominic Dill
Erin Eberhard
Gareth Wyatt
Jabari Lee
Maraida BalaguerBarbosa
Matthew Dalrymple
Michelle Henderson
Merrill Dilbeck
Roberto Infante
Stephany Paredes Mesa
Tiffany-To'Quoya Smith
Civil Engineering
Civil Engineering
Physics & Applied Chemistry
Ecology & Evolutionary Biology
Education
Civil & Environmental Engineering
Chemical Engineering
Civil Engineering & Philosophy
Earth & Atmospheric Science
Civil Engineering
Chemical Engineering
Environmental Engineering
Biology
University or College
University of New Hampshire
University of the Virgin
Islands
University of Illinois at
Urbana-Champaign
University of Technology,
Jamaica
University of South Florida
University of South Florida
Beloit College
University of Michigan
University of South Florida
Florida Gulf Coast University
University of Puerto Rico,
Mayaguez
Bard College
The City College of New York
University of South Florida
University of South Florida
The City College of New York
University of the Virgin
Islands
PI: Maya Trotz, Ph.D.
Associate Professor, Department of Civil and Environmental Engineering
Co-PI: Sarina Ergas, Ph.D.
Professor, Department of Civil and Environmental Engineering
Graduate Coordinator: Christine Prouty
Faculty & Post-Doctoral Mentors
Dr. Norma Alcantar, Chemical Engineering
Dr. Jeffrey Cunningham, CEE
Dr. Nancy Diaz-Elsayed, CEE
Dr. Sarina Ergas, CEE
Dr. Allan Feldman, Secodary Science Education
Dr. Manoj Ram, Nanotechnology Research &
Education Center
Dr. Maya Trotz, CEE
Dr. Meng Wang, CEE
Dr. Daniel Yeh, CEE
Dr. Qiong Zhang, CEE
*CEE-Civil and Environmental Engineering
Graduate Mentors
Eunyoung Lee, CEE
Javkhlan Aruinbaatar, CEE
Karl Payne, CEE
Laura Rodriguez-Gonzalez, CEE
Maureen Kinyua, CEE
Pablo Cornejo-Warner, CEE
Robert Bair, CEE
Ryan Locicero, CEE
Shrikanth Gunti, Mechanical Engineering
Suzanne Boxman, CEE
Veronica Apontes-Morales, CEE
Wen Zhao, Chemical Engineering
NSF REU: Tampa Interdisciplinary Environmental Research (TIER)
USF REU Site, Globalization and Community Health Field School: Combining Social
Science and Engineering
Participant
Roberto Infante Rosado
Major
Chemical Engineering
University or College
University of South Florida
Faculty Mentor
Dr. Sarina Ergas, Civil & Environmental Engineering
Graduate Mentor
Maureen Kinyua, Civil & Environmental Engineering
REU: College of Engineering/Florida Georgia Louis Stokes Alliance for Minority
Participation
Participant
Melinda Moffitt
Major
Biomedical Science and
Research
University or College
University of North Florida
PI: Dr. Ralph Turner, Director of Florida Georgia LSAMP
Co-PI: Dr. Nathan Gallant, Chemical & Biomedical Engineering, Mechanical Engineering
Faculty Mentors
Dr. Robert Frisina, Chemical & Biomedical Engineering, Global Center for Speech and Hearing
Research
Dr. Nathan Gallant, Chemical & Biomedical Engineering, Mechanical Engineering, Cellular
Mechanotransduction and Biomaterials Laboratory
Graduate Mentor
Nicole Febles, Chemical & Biomedical Engineering, Mechanical Engineering, Cellular
Mechanotransduction and Biomaterials Laboratory
Index of Poster Presentations
RET: Functional Materials Research Institute
Page
Poster
Number
Visible Printing of Tissues with Thermo-responsive
Hydrogel
3
7
Sandra Gebhard
Exploration in transmetalation of Co-ZIFs as
precursors for oxygen reduction reaction
electrocatalysts in polymer exchange membrane
fuel cells
4
9
Andria Keene
Sorption of Organic Vapors by Polymers using a
Quartz Crystal Microbalance
5
1
Artemio Perez
Experimental Evaluation of Electrical Response of
Passivating Alloys in Different Electrolytes
6
2
Christina Rutledge
Methods for Capturing Bacteria in Low
Concentrations
7
4
Jordan Lewis
Methods for Capturing Bacteria in Low
Concentrations
7
5
Steven Sanden
Purification of a Fusion Protein for the Treatment
of Cancer Cells
8
10
Pedro Villavicencio
Solving Many-Body Polarization on GPU’s
9
8
Erica Wilkes
FMRI RET 2014-Effect of Viscosity on Fiber
Formation
10
6
Dr. Carol Zavarella
Synthesis of Shape-controlled Bimetallic
Nanoparticles for Catalysis and Photocatalysis
11
3
Presenter
Title
Dr. Megan Faliero
Index of Poster Presentations
NSF RET in Engineering and Computer Science Site: Water Awareness
Research and Education
Page
Poster
Number
Using Technology to Locate Rain Gardens
13
12
Frank Marcantoni
Aquaponics in the Classroom: Sustainability of
Closed-System Multitrophic at Van Buren Middle
School
14
14
Ileana Bermudez Luna
Nitrogen Budget for a Bench-Scale Recirculating
Aquaculture System (RAS)
15
15
Joseph McLaughlin
Weather Stations & Rain Gardens: Linking Climate
Change & Adaptation
16
16
Ken Wood
Effects on gas production and algae growth when
using different concentrations of sugar in
anaerobic digesters
17
17
Kristy Carew
Flood Reduction in Urban Green Spaces Using Rain
Gardens: An Analysis of Media Fill Composition
18
18
Lindsay Guntner
Effectiveness of Biochar and N, P, K-Zeolite
Fertilizer and Soil Amendment
19
19
Maranda Holley
Incorporating Green Infrastructure on an Urban
School Campus
20
20
Mathew Liebelt
Thermal Pretreatment of Algae: Impacts on Biogas
Production
21
21
Mirko I. Soko
Understanding the Engineering Design Process
within the Local Watershed
22
23
Nicole Richards
Rain Garden Performance: Vegetation Monitoring
at King High School
23
24
Stephanie Quintero
Effects on gas production and algae growth when
using different concentrations of sugar in
anaerobic digesters
24
25
Tana (Jackson) Phelps
Marine Aquaponics in the Classroom
25
26
Presenter
Title
Courtney Taylor
Index of Poster Presentations
Page
Poster
Number
Developing Physical Models to Analyze the Water
Quality of Stormwater within Urban Rain Gardens
26
27
Summer Hires
Documentary Film Making as a STEM Learning &
Teaching Tool
27
13
Matt Sestilio
Green Infrastructure Mobile Application
28
22
Andres Parra Garcia
Effectiveness of Biochar and N, P, K-Zeolite
Fertilizer and Soil Amendment
29
11
Presenter
Title
Taryn Kendig
Index of Poster Presentations
NSF REU in Applied Physics
Page
Poster
Number
A new method based on RF impedance technology
and soft ferromagnetic ribbons for real-time
corrosion monitoring
31
31
Janae Bradley
Systematic study of phase transitions in thermoresponsive polymers used in targeted drug delivery
32
32
Jonathan D.
Cuthbertson
Surface Charge Distribution of Poly (Nisopropylacrylamide) After Absorption of Poly-Llysine
33
33
Brad Davis
Investigating the Ripple Phase in Lipid Bilayers
using Molecular Dynamics
34
29
Samuel Hebenstreit
Interactions of lipid vesicles with antimicrobial
peptides and drug molecules
35
37
Luis Martinez
Study of octopod shaped nanoparticles and their
bio-medical applications
36
35
Carl McIntyre
A Study of Two Dimensional Electron Gas Using 2D
Fourier Transform Spectroscopy
37
30
Boeing Smith
Band Energy Modification of Ferroelectric ZnSnO3
for Photovoltaic Applications
38
28
Kim Sonju
Second Harmonic Generation in Non-linear
Metamaterials
39
34
Otho Ulrich
Molecular Dynamics of Bicrystalline Graphene to
Determine Fracture Strength
40
36
Presenter
Title
Izabella Berman
Index of Poster Presentations
NSF REU: Chemistry Summer Enhanced Experience Discovering
Science
Presenter
Title
Page
Poster
Number
Christopher Brewer
Design and the Synthesis of Novel Water-Soluble
Resorcin[4]arenes Decorated with Maltose
monomers via Cu(I)-catalyzed Azide-Alkyne
Cycloaddition (CuAAC)
42
40
Veronika Espinoza
Synthesis and Characterization of Self-Healing
Polycarbonate Polyurethane-Copper-based Paddle
Wheels
43
47
Arielis Estevez
Organocatalysis using BINOL derived phosphoric
acids for an asymmetric hydroboration for a
reductive Aldol reaction
44
39
Nelson GarciaVazquez
D2-symmetric porphyrin synthesis and its
application in the cyclopropanation of α,αdiphenylethylene
45
45
Jermaine Henderson
Sol-gel Niobia based sorbent in CME coupled to
HPLC for enriching organophosphorus pesticides
46
41
Reed Jacobsen
Synthesis of Novel Water-Soluble Recorcin[4]Arene
Cavities Decorated with Sugar Monomers via CuAAC
Cyclo- Addition Allow for Chemical Synthesis in an
Aqueous Environment
47
43
Josue Liriano
CHIRAL PHOSPHORIC ACID-CATALYZED FRIEDELCRAFTS ALKYLATION OF INDOLES AT THE 2POSITION
48
42
Nathan Pickering
Identification of Structural Changes Caused by the
N7-methylguanine Damage in DNA
49
44
Santiago RodriguezOspina
Identification and Characterization of Drosophila
Melanogaster Polyamine N-acyltransferase
50
46
Vinushka Schalk
Development of CHARMMing as a graphical Web
user interface for generalized multi-scale modeling
51
48
Anne-Genevieve Telcy
Optimization of silver shell and silica core
nanoparticles as SERS active substrate
52
38
Index of Poster Presentations
NSF REU in Ubiquitous Sensing
Poster
Page Number
Presenter
Title
Alejandra Vidal
Real-time Pedestrian Tracking in Indoor
Environments
54
50
Edwin Miguel Peguero
Correlation-based Rotation-invariant Biometric
Gait Recognition System Using On-board Sensors
on Android Mobile Phones
55
51
Luis N. Valcourt
Human Fall Detection with Smartphones
56
52
Mark Di Sano
HeartMApp: An Android Application to Reduce
Patient Readmission Rate and Health Risk after a
Cardiovascular Event
57
53
Steven Díaz Hernández
Dynamic Background Subtraction for Fall
Detection System using a 2D Camera
58
54
Index of Poster Presentations
NSF REU: Tampa Interdisciplinary Environmental Research
Presenter
Title
Page
Poster
Number
Allison Wood
Kinetics of the Anaerobic Co-Digestion of Algae
and Waste Activated Sludge
60
56
Ariane Ramsundar
Adsorption studies on a Clinoptilolite packed
column for treatment of Septic Tank Effluent
61
57
Chinelo Agbim
Performance of an anaerobic membrane reactor
for the treatment of food waste
62
58
Christopher Roberts
Mobile Technology & Dengue: A water based
human centric approach to beat dengue in the
Caribbean
63
59
Clyde E. Joseph
Florida native plants for Rain Gardens- A
comparison of effective phosphorus removal from
stormwater runoff
64
60
Donald Dominic Dill
Zeo-Pack for Nitrogen Removal and Recovery
65
62
Erin Eberhard
The effect of using exotic animal dungs as inocula
for the anaerobic digestion of food waste
66
63
Gareth Wyatt
Mentoring Styles in Formal Mentorships During a
Summer Environmental Engineering REU
Program
67
64
Jabari Lee
Plant Selection Process for Use in Rain Gardens
68
65
M. Peyton Dilbeck
Impact of Thermal Pretreatment on SwineManure’s Biogas Production in an Anaerobic
Digester
69
69
Maraida BalaguerBarbosa
An Evaluation of the Energy and Greenhouse Gas
Emissions of Onsite Wastewater Treatment
Systems
70
66
Matt Dalrymple
Degradation of Solid Phase Extraction (SPE)
Cartridges
71
67
Michelle Henderson
Graphene Doped Metal Oxide Nanoparticles for
Methyl Orange Removal
72
68
Index of Poster Presentations
Presenter
Title
Page
Poster
Number
Stephany Paredes Mesa
Photo-degradation of Dispersed Oil
73
70
Tiffany-To’Quoya
Smith
Changes in ammonia, nitrite, and nitrate in bench
scale marine aquaponic systems
74
71
Cove Capodice
Absorbtion of Ammonium in Two Biofilter
Mediums
75
61
Index of Poster Presentations
USF REU Site, Globalization and Community Health Field School:
Combinging Social Science and Engineering
Presenter
Title
Page
Poster Number
Roberto Infante
Rosado
An Overview of Animal Waste Water
management: Biodigesters in the Monteverde
Zone of Costa Rica
76
55
REU: College of Engineering/Florida Georgia Louis Stokes Alliance for
Minority Participation
Presenter
Title
Page
Poster Number
Melinda Moffitt
Effect of Biomaterial Stiffness on Feeder Cell
Growth
77
49
Bio-Sketches and Abstracts
1
NSF RET: Functional Materials Research Institute
2
RET: Functional Materials Research Institute (FMRI)
Dr. Megan Faliero
1. Bio Sketch
1.1. Home Institution: Durant High School
1.2. Grade or level taught: 9-12
1.3. Subjects taught: AP Biology and Biology Honors
1.4. USF Research Mentor: Dr. Toomey, Dr. Gallant, Olukemi
Akintewe
2. Abstract
Viable Printing of Tissues with Thermo-responsive Hydrogel
Megan Faliero1, Olukemi Akintewe2, Nathan Gallant3
, Ryan Toomey2
1Biology,
2USF
Durant High School
Chemical & Biomedical Engineering
2USF Mechanical Engineering
megan.faliero@sdhc.k12.fl.us
Keywords: thermo-responsive polymer, PNIPAAM, cell printing, cell release, tissue engineering
Tissue regeneration is an exciting field of research that is showing advances from the traditional
methods of scaffolding, where immune system responses have been a problem, to using functional
materials to create viable three-dimensional tissues. The use of a thermally responsive hydrogel,
poly N-isopropylacrylamide (PNIPAAM) as a cell culture platform allows the release of intact cells
in defined geometries or sheets without the damage to the extracellular matrix (ECM) that
traditional release techniques, such as the use of enzymes, have caused. PNIPAAM has a lower
critical solution temperature (LCST) of 32*C; below this temperature, the polymer becomes
hydrophilic and swells while above this temperature, the polymer becomes hydrophobic and
collapses. As cell release platform, when PNIPAAM swells, the strain between the polymer and the
cells causes the cells to detach intactly from the polymer due to disruptions of the cell matrix
caused by the expansion of the polymer. In this study, we have examined the feasibility of printing
viable cells for building robust three-dimensional tissues.
3
RET: Functional Materials Research Institute (FMRI)
Sandra Gebhard
1. Bio Sketch
1.1. Home Institution: Brandon High School
1.2. Grade or level taught: 9-12
1.3. Subjects taught: Chemistry
1.4. USF Research Mentor: Dr. Shengqian Ma
2. Abstract
Exploration in transmetalation of Co-ZIFs as precursors for oxygen
reduction reaction electrocatalysts in polymer exchange membrane fuel
cells
Sandra Gebhard1, Kia Williams2, Dr. Shengqian Ma 2
1Chemistry,
2USF
Brandon High School
Department of Chemistry
Sandra.Maurer@sdhc.k12.fl.us
Keywords: Metal-Organic framework, fuel cell, catalyst, transmetalation
The potential of fuel cells as a component of alternative energy systems has encouraged research in
optimizing their performance and cost-effectiveness. Platinum group metals are currently the most
widely used electrocatalysts for the oxygen reduction reaction (ORR) at the cathode in polymer
exchange membrane fuel cells (PEMFCs). Since reserves of platinum group metals are limited and
expensive, explorations into alternative materials have been at the forefront of fuel cell research. In
this study, a zinc based Metal-Organic framework (ZIF-7) will be modified as a self-sacrificing
precursor for an ORR catalyst. To determine the effect on the catalytic activity and potentially gain
an understanding of the mechanism behind the ORR reaction in this system, zinc will be exchanged
with varying amounts of cobalt.
4
RET: Functional Materials Research Institute (FMRI)
Andria Keene
1. Bio Sketch
1.1. Home Institution: Strawberry Crest High School
1.2. Grade or level taught: 9-12
1.3. Subjects taught: Marine Science I Honors, Marine Science II
Honors, Biology Honors
1.4. USF Research Mentor: Dr. Scott Campbell
2. Abstract
Sorption of Organic Vapors by Polymers using a Quartz Crystal
Microbalance
Andria Keene1, Ana Stringari de Castro2, Dr. Scott Campbell2
1Science,
2USF
Strawberry Crest High School
Chemical & Biomedical Engineering
Andria.keene@sdhc.k12.fl.us
Keywords: polymer, sorption, quartz, benzene, quartz crystal microbalance, organic vapors,
solubility, poly(styrene-butadiene), poly(iso-butylene), polydimethylsiloxane, and poly(butadiene)
The ability to measure the solubility of solvents in polymers is an important aspect in chemical
engineering especially in the field of industrial applications where it is essential to be able to
remove or add materials based on the desired properties. Sorption rates are usually measured by
comparing the weight before and after absorption however, diffusion of molecules through
polymers can be a time consuming process. A reduction in the thickness of the polymer yields faster
results but makes measuring of the smaller mass more difficult. The use of a quartz crystal
microbalance has proven helpful due to its sensitivity and quickness in measuring. The
piezoelectric properties of the quartz allow it to be used to measure very small changes in mass.
In this technique a quartz crystal is coated with a thin layer of polymer. The frequency of the quartz
is tested before and after the coating to be sure that the desired shift is attained. Next, a carrier gas
bubbles through and picks up a solvent. It is then carried across and absorbed by the polymer
within a temperature controlled cell. When equilibrium in the polymer and gas stream is reached,
the additional shift is noted.
The polymers that are examined during this experiment include poly(styrene-butadiene) and
polydimethylsiloxane. The carrier gas is nitrogen and the solvents are benzene and cyclohexane.
5
RET: Functional Materials Research Institute (FMRI)
Artemio Perez
1. Bio Sketch
1.1. Home Institution: Plant City High School
1.2. Grade or level taught: 11-12 Grade
1.3. Subjects taught: Honors & AP Physics
1.4. USF Research Mentor: Dr. Nathan Crane
Experimental Evaluation of Electrical Response of Passivating
Alloys in Different Electrolytes
Artemio Perez1, Qi Ni2, Dr. Nathan B. Crane2
2Mechanical
1Plant
City High School
Engineering, University of South Florida
artemio.perez@sdhc.k12.fl.us
Keywords: electrowetting (EW), electrowetting-on-dielectric (EWOD), continuouselectrowetting (CEW), bidirectional, electro-chemical, diode, electrofluidic, microfluidic,
droplet
Continuous electrowetting (CEW) is an evolution in conventional electrowetting (EW) in
which two electrodes were used instead of multiple electrodes. CEW allows a more rapid
transportation of micro-droplet as well as a more simplified control scheme. In CEW, metal
electrodes are combined with electrolyte solutions to create a series of diodes by
etching/passivating the metal in different location of the droplet. For long term reliability,
CEW requires the use of metal electrodes and electrolyte solutions that can not only
withstand chemical etching but also provide diode behavior in repeated electric cycling.
Valve metals such as aluminum and titanium have been known to have high chemical
resistivity due to their rapid forming native-oxide layers. Metal electrodes were tested with
various electrolyte solutions to investigate the long-term reliability for use in CEW. Cyclic
voltammetry were performed while simulating a droplet in CEW system. The IV
measurements showed diode like behavior for all electrolytes over long term use. Sodium
sulfate (Na2SO4) and titanium proved to withstand long term use up to 2000 cycles with no
sign of degradation.
6
RET: Functional Materials Research Institute (FMRI)
Christina Rutledge
1. Bio Sketch
1.1. Home Institution: Strawberry Crest High School
1.2. Grade or level taught: 9th-12th
1.3. Subjects taught: IB Biology & AP Biology
1.4. USF Research Mentor: Anna Pyayt
Jordan Lewis
1. Bio Sketch
1.5. Home Institution: University of South Florida
1.6. Major: Middle Grades Math Education
1.7. Professional Goal: 5-9 Math Educator
1.8. USF Research Mentor: Anna Pyayt
2. Abstract
Methods for Capturing Bacteria in Low Concentrations
Christina Rutledge1, Jordan Lewis2, Karthik Raj3, Edikan Archibong3, Anna
Pyayt3
Department, Strawberry Crest High School
Department College of Education, University of South Florida
3Chemical and Biomedical Engineering Department, University of South Florida
Christina.rutledge@sdhc.k12.fl.us
jlewis17@mail.usf.edu
2Math
1Science
Keywords: Bacteria, Detection, Microfluidics
Minor bacterial infections can result in sepsis, a dangerous condition taking the lives of 215,000
Americans each year. Detection of bacteria, especially in low concentration, is a challenging task
that can take up to several days. Specifically capturing bacteria from a biological sample would
result in a concentrated filtrate that could be analyzed for the presence of bacteria. Here, we
explored methods for bacterial capturing using vancomyin and a silicon nitride microfluidic chip.
Optical microscopes were used to demonstrate their capturing abilities on various bacteria.
Successful identification of contaminated samples would lower the risk of sepsis in patients.
7
RET: Functional Materials Research Institute (FMRI)
Steven Sanden
1. Bio Sketch
1.1. Home Institution: Middleton High School
1.2. Grade or level taught: 11th and 12th grade
1.3. Subjects taught: Chemistry and Earth/Space Science
1.4. USF Research Mentor: Dr. Piyush Koria
2. Abstract
Purification of a Fusion Protein for the Treatment of Cancer Cells
Steven Sanden1, Dagmara Monfort2 and Piyush Koria2
1Chemistry/Science Department, Middleton High School
2USF Chemical & Biomedical Engineering
steven.sanden@gmail.com
Keywords: KLAKLAK, peptide, ELP
(KLAKLAK)2 is a cationic α-helix peptide that is well known to cause cell death once it is
internalized by cells. In this work, we purified (KLAKLAK)2 fused to elastin-like
polypeptides (ELPs). ELPs are protein-based polymers which consist of the repetitive
sequence VPGXG where X could be any amino acid but proline. ELPs are thermoresponsive
polymers which solubility changes at a particular temperature called transition
temperature. It is this transition temperature that allows for the purification of the fusion
(KLAKLAK)2-ELP using the well documented method of inverse transition cycling (ITC).
After the purification of the (KLAKLAK)2-ELP, lung cancer cells (A549) were treated with
the fusion and a live/dead assay was performed to test its cytotoxic effect. The results
obtained clearly showed that (KLAKLAK)2-ELP causes cell death at high concentrations.
8
RET: Functional Materials Research Institute (FMRI)
Pedro Villavicencio
1. Bio Sketch
1.1. Home Institution: Steinbrenner High School and PHSC
1.2. Grade or level taught: 9-12 and H.S. graduates
1.3. Subjects taught: Mathematics, Computer Science
1.4. USF Research Mentor: Brant Tudor, Christian R. Cioce, Brian
Space.
2. Abstract
Solving Many-Body Polarization on GPU’s
Pedro Villavicencio1, Brant Tudor2, Christian R. Cioce2, Brian Space2
1Mathemartics
Department – Steinbrenner High School
of Chemistry
2Department
Pedro.Villavicencio@shdc.k12.fl.us
Keywords: Algorithms, Design, CUDA, GPGPU, MOF, Parallel Programming, Polarization
Massively
Parallel
Monte
Carlo,
an
in-house
computer
code
available
at
http://code.google.com/p/mpmc/, has been successfully utilized to simulate interactions between
gas phase sorbates and various metal-organic materials. In this regard, calculations involving
polarizability were found to be critical, and computationally expensive.
Although GPGPU routines have increased the speed of these calculations immensely, in its original
state, the program was only able to leverage a GPU’s power on small systems. In order to study
larger and evermore complex systems, an attempt to modify the program model using new parallel
programming techniques was performed. It was expected that the new techniques, while providing
similar or better performance, will solve limitations related to convergence times, system size and
scalability.
After implementation, the new techniques yielded results that were more or less five percent
accurate, lower than the ideal. In this project, another attempt is made to improve the accuracy of
the model which will subsequently solve the aforementioned limitations.
9
RET: Functional Materials Research Institute (FMRI)
Erica Wilkes
1. Bio Sketch
1.1 Home Institution: C. Leon King High School, Tampa, FL
1.2 Grade or level taught: 9-12
1.3 Subjects taught: AP Biology, Chemistry H, Biology H
1.4 USF Research Mentor: Sylvia W. Thomas, Ph.D.
2. Abstract
FMRI RET 2014-Effect of Viscosity on Fiber Formation
Erica Wilkes1, Rasudha Muppaneni2, Manopriya Subramanyam2, Brian Bell2,
Hruday Katakam2, Samuel Perez2, Sylvia W. Thomas, Ph.D.2
1Science
Department, King High School
of Electrical Engineering
2Department of Electrical Engineering
2Department
Erica.Brogna@sdhc.k12.fl.us
Keywords: Electrospinning, polymer, polystyrene, viscosity, nanofiber
Electrospinning is a simple, repeatable process that involves placing a polymer solution, or melt,
into a syringe to which an electric field is then applied. The applied electric potential overcomes
the surface tension of the polymer solution. A polymer jet is then ejected from the syringe needle
tip and is deposited onto the collector as a non-woven web. The repulsive electrostatic forces
create bending instabilities that cause the jet to spiral as its traveling to the collector. To minimize
the instability, the jet undergoes plastic stretching, which reduces its diameter, thus forming
extremely thin fibers that range in diameter from nanometers to a few microns. If a low polymer
concentration is used, fibers may not form due to the jet breaking down into droplets as an
electrospray instead. Changing the concentration or molecular weight of the polymer changes the
viscosity, thus changing the diameter of the fiber and the number of beads that form on the fiber.
While there are many other parameters that affect electrospinning and fiber formation, such as the
properties of the polymer solution including solution conductivity, solution surface tension and
solvent volatility, as well as infusion rate, applied voltage, distance to the collector, motion of the
collector, humidity and temperature, our focus is on researching how viscosity affects fiber
formation and functionality. The parameters for the polymer solutions that will create optimal
fibers for water filtration, piezoelectric applications, thermal reflective military applications and
self-healing capability for military applications can then be determined.
10
RET: Functional Materials Research Institute (FMRI)
Dr. Carol Zavarella
1. Bio Sketch
1.1. Home Institution: Hillsborough Community College
1.2. Grade or level taught: College Level Courses
1.3. Subjects taught: Mathematics and Statistics
1.4. USF Research Mentor: Dr. John Kuhn
2. Abstract
Synthesis of Shape-controlled Bimetallic Nanoparticles for Catalysis and
Photocatalysis
Dr. Carol Zavarella1, Jon Pickering2, Dr. John Kuhn2
1Mathematics
2Chemical
& Statistics – Hillsborough Community College
& Biomedical Engineering – University of South Florida
2Chemical & Biomedical Engineering
czavarella@hccfl.edu
Keywords: catalysis, materials science, nanotechnology, nanoparticles, nanocubes
Nanostructures of a particular size and shape have been shown to improve catalytic activity and
selectivity. The nanocube, due to its sharp corners and edges and of a particular size (30-70nm),
have shown promise in a variety of applications including plasmonics and catalysis due to its
unique optical properties. In plasmonics, silver nanocubes serve as guides to direct light to specific
locations with precision. Although silver nanocubes have excellent optical properties, they have
limited catalysis capability, particularly in CO2 conversion. Ruthenium particles, however, have
excellent catalysis capabilities and are more efficient at activating CO2. The purpose of this study is
to epitaxially grow the ruthenium onto the silver substrate to take advantage of each metals’
individual characteristics to enhance catalysis. After the ruthenium is coated onto the silver
nanocubes, a sample is deposited on an oxide semiconductor to test the catalysis.
11
RET in Engineering and Computer Science Site:
Water Awareness Research and Education
12
RET in Engineering and Computer Science Site: Water Awareness Research and Education
Courtney Taylor
1. Bio Sketch
1.1.
1.2.
1.3.
1.4.
1.5.
School: Learning Gate Community School
Grade Taught: 5th Grade
Subjects Taught: Math and Science
USF Research Mentors: Ryan C. Locicero & Dr. Maya Trotz
Professional Goals: Develop Application appropriate for locating
rain garden/strom water collection areas in need to involve the
community surround the Tampa Bay area through the use of
local schools.
2. Abstract
Using Technology to Locate Rain Gardens
Courtney Taylor1, Ryan Locicero2, Maya Trotz2
1Learning
2Civil
Gate Community School
& Environmental Engineering, University of South Florida
Courtney14@mail.usf.edu
Keywords: Rain gardens, K-12 Education, S.T.E.M.
The USF-WARE Research Experience for Teachers (RET) program provides teachers with a
platform to develop a comprehensive understanding of green infrastructure (GI) practices and the
tools to integrate grade specific content into the classroom. The RET program has developed
curriculum for middle grades, (6-8) that will be used as a framework for the integrating GI concepts
and standards into the elementary classroom.
A rain garden is a type of GI that introduces students to the concepts of decentralized
stormwater management (e.g. Common Core-SC.5.E.7.2). The design and construction of a rain
garden will be integrated into the state mandated curriculum throughout the school year.
Maintenance and performance of these systems is an important factor to consider and will be
integrated into additional lessons/activities post construction. In addition, an interactive website or
social media medium (e.g. app) will be developed to challenge students to locate rain garden
Hotspots. Through this technology, students will apply what they've learned during their school
campus rain garden build to involve their parents and local community. Future work includes the
extension of the school based rain garden curriculum to the local community where stakeholders
can find suitable areas for implementing rain gardens.
13
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Frank Marcantoni
1. Bio Sketch
1.1. School: Van Buren Middle School
1.2. Grades Taught: 7th and 8thh
1.3. Subjects Taught: Advanced and Gifted Mathematics, Algebra
1.4. USF Research Mentors: Maya Trotz and Suzanne Boxman
1.5. Professional Goals: My long term professional goal is to continue
advance within the district and to incorporate inquiry-based learning
with mathematics in order to increase understanding and retention. My
short term professional goal is to have the best instructional year of my
career this year as a brand-new mathematics teacher and to continue to
work with USF WARE RET.
2. Abstract
Aquaponics in the Classroom: Sustainability of Closed-System
Multitrophic at Van Buren Middle School
Frank Marcantoni1, Suzanne Boxman2, Dr. Maya A. Trotz2
1Van
2Civil
Buren Middle School
and Environmental Engineering, University of South Florida
frank.marcantonisr@sdhc.k12.fl.us
Keywords: Aquaponics, stormwater, K-12 education, STEM
An aquaponics system combines aquaculture (farming fish) with hydroponics (growing
plants in mineral nutrient water with no soil) so that the plants grow on the effluent from the fish
tanks. This system, that closely mimics nature, is a method to grow lean protein and fresh
vegetables. There are vast amounts of information available on fresh water aquaponics, but little on
marine aquaponics. Living in a costal environment, marine science is important to our community
and students and this research aims to integrate that through an engineered classroom model. A
larger study at the MOTE Aquaculture Research Park is underway that integrates red drum fish
with potential marketable foods in Florida, sea purslane and salt wort.
During the 2014-2015 school year my students will be utilizing a multitrophic level
aquarium system for building and maintaining a marine aquaponics system. Initially, the students
will engage with an e-module specifically designed for this lesson. The students will be given a
budget and will select the flora and fauna based on the aquaponics design with the purpose of
minimizing cost and maximizing harvest yield. Ideally, the program will incorporate variables such
as cost for the livestock, time to harvest, nutritional requirements, mortality rate, and susceptibility
to disease, and value of the plant species.
After having developed a consensus on types of living organisms and plants, the students
will create an actual marine aquaponics system with their selections. Once created, they will
monitor and measure temperature, salinity, ammonia, pH, nitrites, and nitrates using test kits
available at an aquarium store, and will adjust and maintain the system accordingly. They will
maintain a shared database for reflecting and sharing information. They will also conduct a cost
benefit analysis of their systems and compare it to traditional sources of food production.
14
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Ileana Bermudez Luna
1. Bio Sketch
1.1.
1.2.
1.3.
1.4.
2. Abstract
School: Freedom High School
Grades Taught: 10th, 11th & 12th
Subjects Taught: Chemistry
USF Research Mentors: Laura Rodriguez-Gonzalez & Dr. Sarina
Ergas
1.5. Professional Goals: She recently graduate from the University of
South Florida with a B.S. in Science Education. As a new science
teacher, her primarily goal is to use all the resources, experiences
and new knowledge obtained throughout this program to develop a
yearlong Project for her high school students, focusing on the
chemistry aspects of a Recirculating Aquaculture System (RAS)
Nitrogen Budget for a Bench-Scale Recirculating Aquaculture System
(RAS)
Ileana Bermudez Luna1, Laura Rodriguez-Gonzalez2, Dr. Sarina Ergas2
1Freedom
2Department
High School
of Civil and Environmental Engineering, University of South Florida
ileana.bermudezluna@sdhc.k12.fl.us
Keywords: recirculating aquaculture system (RAS), nitrogen budget, sustainability, denitrification
Recirculating Aquaculture Systems (RAS) are a growing practice in the United States due to
their economic benefits. When implementing this type of system it is important to consider
fundamental components for efficient wastewater treatment, such as solid waste and conversion of
ammonium and nitrite, to nitrates. This research focuses on the determination of the Nitrogen
Budget (NB) for our bench scale RAS. In our system, synthetic aquaculture wastewater is the main
source of ammonium-nitrogen; with the addition of oxygen, nitrification occurs in a moving bed
bioreactor (MBBR) and finally passes through a denitrifying column (AD) with an external flow of
molasses as a carbon source, needed for the decomposition of nitrates to nitrogen gas. Samples
were taken every 48 hours from the different ports within our bench scale RAS. Water quality
parameters such as pH, dissolved Oxygen (DO), conductivity, alkalinity and Chemical Oxygen
Demand (COD), were studied to better understand the effectiveness of our RAS. Tests for nitrites,
nitrates, ammonia and Total Nitrogen (TN) were performed to determine the input and output of
nitrogen across the system and finally calculate the Nitrogen Budget for our bench scale RAS.
Preliminary results show that after MBBR the amount of ammonium-nitrogen reduced significantly
and levels of nitrate-nitrogen increased, indicative of nitrification. After the denitrification column
the system’s nitrate concentrations decreased, inferring denitrification, but an increase in COD was
observed. Based on these preliminary results, some of the recommendations for system’s
optimization are: 1) modify the molasses input to improve denitrification and control COD
concentrations, and 2) optimize oxygen input mechanism to enhance nitrification.
15
RET in Engineering and Computer Science Site: Water Awareness Research and Education
Joseph McLaughlin
1. Bio Sketch
1.1.
School: Howard W. Blake High School
1.2.
Subject Taught: Mathematics
1.3.
Grade Level: 9-12
1.4.
USF Research Mentor: Maya Trotz, Ph.D; Ryan Locicero
1.5.
Professional Goals: To incorporate real-world experience into
Algebra 2 curriculum and instruction, in order to help foster student
interenst into the fields of science, technology, engineering, and
mathematics.
2. Abstract
Weather Stations & Rain Gardens: Linking Climate Change & Adaptation
Joseph McLaughlin1, Ryan Locicero2, Maya Trotz2
1Howard
2Civil
W. Blake High School
and Environmental Engineering, University of South Florida
joseph.mclaughlin@sdhc.k12.fl.us
Keywords: weather station, rainfall intensity, climate change, flooding, rain garden, green
infrastructure
Climate change predictions of changes in the amount of rainfall and the intensity of rainfall
directly link to flooding, especially in low lying coastal areas. Adaptation to climate change in this
case will usually look at interventions that help to reduce the impact of flood events on local
communities. In the Tampa Bay region, stormwater runoff contributes to pollutant loads in the Bay
and green infrastructure, rain gardens in particular, are being used to handle runoff closer to the
source. Through the USF WARE RET program, these are being introduced to K-12 communities and
implemented on school campuses as project based activities that also create Green Space Based
Learning environments. This research aims to introduce the topic of adaptation as it relates to
changes in rainfall as well as develop student understanding of the implications this has on the local
area under near term and predicted long term variability. It will do so through the installation and
use of a weather station at the school, observation of the local school environment during rainfall
events, eventual design and construction of a rain garden, and evaluation of its limitations for
handling different amounts and intensities of rainfall. This lesson plan can be used to better design
a rain garden given actual weather data for the particular area/site instead of the generic design
criteria of 1” of rainfall. It can also be used to investigate air pollutant loads into the rain gardens
and urban environment and the weather station data can be linked to plant species survivability.
16
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Ken Wood
1. Bio Sketch
1.1.
1.2.
1.3.
1.4.
1.5.
School: Wharton High School
Grades Taught: 9th – 12th
Subjects Taught: AP Biology, Zoology, and Biology Honors
USF Research Mentor: Jorge Cabria and Daniel Yeh
Professional Goals: My long term professional goal is to obtain a
doctorate in Biology and earn a faculty position at a college or
university. My short term professional goal is to continue
working on my masters degree in Biology and gain experience in
scientific research. I plan to continue developing curriculum in
the Biological Sciences and mobilize essential science practice
into the classroom for my students.
2. Abstract
Effects on gas production and algae growth when using different
concentrations of sugar in anaerobic digesters
Ken Wood1, Jorge Calabria2, Daniel Yeh2
1Wharton
2Department
High School
of Civil and Environmental Engineering, University of South Florida
kenneth.wood@sdhc.k12.fl.us
Keywords: anaerobic digester, photobioreactor, algae
The goal of this investigation is to test the effects on gas production and algae growth after
adding different concentrations of sugar to anaerobic digestors whose gas by products are being
pumped into photobioreactors. It is suspected that with greater concentrations of sugar the
anaerobic digesters will produce more gas by products (methane and carbon dioxide). This gas will
be pumped into a photobioreactor filled with algae. It is unknown if the increased amount of gas
coming from the anaerobic digesters will cause an increase or decrease in algae growth. Algae will
grow more with increased access to carbon dioxide for photosynthesis. However, an increase in
carbon dioxide will also cause changes in pH. The increased acidity may hinder algae growth. The
goal of this experiment is to help determine the parameters which encourage ideal algae growth in
photobioreactors.
17
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Kristy Carew
1. Bio Sketch
1.1 School: C. Leon King High School
1.2 Grades Taught: 10th – 12th
1.3 Subjects Taught: AP Environmental Science
1.4 USF Research Mentor: Maya Trotz and Ryan Locicero
1.5 Professional Goals: To continually add to my educational
experience and success by continually improving my skills through
profesional development and continuing education opportunities.
2. Abstract
Flood Reduction in Urban Green Spaces Using Rain Gardens: An Analysis
of Media Fill Composition
Kristy Carew1, Ryan Locicero2, Dr. Maya A. Trotz2
1C. Leon King High School
2Civil and Environmental Engineering, University of South Florida
kristy.carew@sdhc.k12.fl.us
Keywords: rain garden, hydrologic cycle, media, green infrastructure, infiltration rate
Urban rain gardens are a type of green infrastructure that reduces flooding during storm
events. The Green Space Based Learning model was developed at the University of South Florida to
integrate STEM education in K-12 schools through green infrastructure implementation on the
school campus. In June 2014 a rain garden was installed at the C. Leon King High School in Tampa,
Florida by identifying high flood zones on the school campus that were a nuisance during school
hours. The rain garden contained pea gravel, sand, top soil and mulch, all of which allowed water to
infiltrate faster than the soil that was there previously. This research focuses on identifying the
optimum media components and quantities based primarily on infiltration rates.
Students will design a model that mimics a rain garden system that allows water to
percolate through different media layers and use this model to compare infiltration rates of various
media composition and then select media most suitable for their school site. Firstly, they will use
readily available soil test kits to classify the soil types in the areas that flood at the school, the media
in the existing rain garden, and any new media materials. They will then design a model that tests
infiltration rates and plant growth rates. Plants will either be Florida natives, edibles, or mosquito
repellant type species. They will then select the optimum media mix that would limit flooding and
support plant growth, assuming they support the notion that plants in the rain garden add value to
their school. Availability, cost, and ability to remove contaminants are also important and can be
added on as variables for students to research. The selected media will be applied to a new rain
garden on the campus later in the school year along with their plants. This student-based learning
module educates students on the types of soil, the role these soils play on the hydrologic cycle, and
the impact impervious substrates have on stormwater runoff. This learning module will teach
students about sustainability, green infrastructure and water quality.
18
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Lindsay Guntner
1. Bio Sketch
School: Paul R. Wharton High School
Grades Taught: 9-12
Subjects Taught: Biology, Chemistry and Biotechnology
USF Research Mentor(s): Sarina Ergas, Ph.D.; Veronica Morales
Apontes and Adib Amini
1.5. Professional Goals: I aim to improve the quality and direction of
STEM education by aiding students to a higher level of thinking within the
curriculum through authentic experiences, such as what I have had the
privilege to endure for the last two summers, as well as through inquirydriven instruction. I have done so already by structuring my classroom with
a combination of reformed methods and traditional pedagogy to provide my students with the most
out of each course I teach.
1.1.
1.2.
1.3.
1.4.
2. Abstract
Effectiveness of Biochar and N, P, K-Zeolite Fertilizer and Soil
Amendment
Andrés Parra Garcia1, Lindsay Guntner1 Veronica Apontes-Morales2, Adib
Amini2, Sarina Ergas, Ph.D.2
1Paul
2
R. Wharton High School, Tampa, Florida.
Department of Civil & Environmental Engineering, University of South Florida
lguntner@mail.usf.edu
Keywords: nutrient recovery, biochar, N, P, K,-zeolite, sustainable agriculture
The swine industry makes up the majority of the agricultural enterprise in the United
States; nearly 40% of the world's meat production comes from this industry. As the demand
increases, concentrated animal feeding operations (CAFOs) are becoming more widespread which
leads to concern surrounded by the potential threat of the discharge of nutrient-rich wastes
entering into nearby streams and other receiving bodies. The addition of biochar, a carbon-rich
substance derived from biomass, as a soil amendment has been well studied to improve soil fertility
and improve water retention to prevent nutrient leaching. The objective of this study is to: (1)
evaluate the impact of biochar on the quality of soils after the application of various fertilizing
agents; (2) evaluate the overall productivity of the ryegrass plant; and (3) determine the
agricultural value of this proposed system. Swine manure was anaerobically digested and produced
a nutrient-rich effluent where the liquid portion (centrate) was sent through an ion exchange
method to generate N,P,K-Zeolite fertilizer and the biosolids were converted, through pyrolysis,
into biochar. Soil and plant tissue will be tested against a variety of parameters such as: soil pH,
nutrient leaching, soil nutrient availability as well as the plant’s growth yield. This research will
contribute to further work in developing a system that utilizes sustainable products from wastes
that will offset the need to create conventional fertilizers.
19
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Maranda Holley
1. Bio Sketch
1.1. School: John Quincy Adams Middle School
1.2. Grades Taught: 6th & 7th
1.3. Subjects Taught: Comprehensive Science
1.4. USF Research Mentor: Ryan Lociceo and Dr. Maya Trotz
1.5. Professional Goals: During my first year of teaching, I was able to
incorporate the research experience gained through the WARE-RET
program via a scaffolding lesson plan regarding various aspects of green
infrastructure. I hope to integrate the research experience gained in my
second year to encourage my students to pursue a career in a STEM field
and promote multidisciplinary ideals. Further, the development of a STEM club to continue the
projects goals of educating the school and local community about green infrastructure.
2. Abstract
Incorporating Green Infrastructure on an Urban School Campus
Maranda Holley, M.A.T.1, Ryan C. Locicero2, Maya A. Trotz, Ph.D.2
2Civil
1John Quincy Adams Middle School
and Environmental Engineering, University of South Florida
Maranda.Holley@sdhc.k12.fl.us
Keywords: Stormwater management, Green Infrastructure, Nature of Science, Engineering Design
Process, raingarden
This project cultivates a multidisciplinary approach by incorporating a science, technology,
engineering, and mathematics (STEM) discipline, through the integration of the nature of science,
the Engineering Design Process (EDP), and curriculum (Next Generation Sunshine State Standards).
Students partake in the STEM processes to design green infrastructure. Students gain an
understanding of Florida’s unique ground structure, water cycle, effects of stormwater via nature of
science ideals. In June 2014 a rain garden was designed and installed by students at Adams Middle
School. Students determined the areas on campus in need of green infrastructure to assist with
stormwater management, in addition to, ensuring possible solutions fit within the scope and budget
of the project.
Understandings of STEM ideals are promoted by identifying an existing problem,
researching possible solutions and alternative green infrastructure solutions, then ultimately
implementing a viable and sustainable solution. Further, an introduction of concepts, such as, cost
estimates, design bids/argumentation, biological treatment, and low impact development is gained
using the EDP. The overarching green infrastructure design model is a raingarden. The raingarden
concept is centered on the importance of stormwater management, as well as, reducing the
environmental impact stormwater has on the campus and the surrounding community.
Additionally, plant selection for the raingarden benefits the local flora and fauna of Florida.
20
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Mathew Liebelt
1. Bio Sketch
1.1.
1.2.
1.3.
1.4.
1.5.
School: Plant City High School
Grades Taught: 9th
Subjects Taught: ESE Biology
USF Research Mentor: Sarina Ergas and Meng Wang
Professional Goals: I plan to use the knowledge and experience
gained from this WARE-RET program to better implement
science and engineering research practices into my High School
Biology classroom to provide an authentic environment for my
students to learn, experience and participate in real-world
science and engineering.
2. Abstract
Thermal Pretreatment of Algae: Impacts on Biogas Production
Matthew Liebelt1, Meng Wang2, Sarina Ergas2
1Plant
2Department
City High School
of Civil and Environmental Engineering, University of South Florida
Matthew.Liebelt@sdhc.k12.fl.us
Keywords: Algae, methane, anaerobic digestion, pretreatment, biogas
Algae grows rapidly on wastewater and a low-cost method of using it to produce bioenergy
is through anaerobic digestion to produce methane gas. The main goal of this study is to analyze
the effectiveness of thermal pretreatment on improving biogas production of the anaerobic
digestion of algae. Substrate pretreatment increases the solubility of the organic matter, making it
more available to anaerobic microorganisms.
Biological methane potential (BMP) of thermal pretreated substrates at mesophilic
conditions (35 oC) was measured in batch reactors. Algae were pretreated at 70 oC and 90 oC for 30
minutes and 121oC for 20 minutes (preliminary studies found these conditions optimal in terms of
cost and Chemical Oxygen Demand). Anaerobically digested sewage sludge from Clearwater
Wastewater Treatment Plant seeded (inoculum) the anaerobic digestion process. Controls were
digested algae without pretreatment. Initial tests were conducted to analyze the properties of each
sample, including the following: pH, Total Solids (TS), Volatile Solids (VS), Volatile Fatty Acids
(VFA), Phosphate (PO43-), Ammonia (NH3+ –N), Total Nitrogen (TN), Total Phosphorous (TP), Total
Chemical Oxygen Demand (tCOD), and Soluble Chemical Oxygen Demand (sCOD). The biogas
production was measured throughout a 15 day period. The sample heated to 121 oC and 90 oC
improved total gas production by roughly 23% and 13% over the control respectively. There was
no significant difference in biogas production between the sample heated to 700C and the control.
The implications of these results are twofold; first, algae can be utilized as a biofuel through the
anaerobic digestion process, and second, the biogas production can be increased through thermal
pretreatment.
21
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Mirko I. Soko
1. Bio Sketch
1.1.
1.2.
1.3.
1.4.
1.5.
2. Abstract
School: Adams Middle School
Grades Taught: 8th
Subjects Taught: Comprehensive Science
USF Research Mentor:Ryan Locicero & Dr. Maya Trotz
Professional Goals: During the past year and a half I completed the
Alternative Certification Program (ACP) and the requirements
for Project S.M.A.R.T through the Hillsborough County Public
School System. The WARE-RET allowed me to integrate my
engineering background, content knowledge, and pedagogy. I
will incorporate the ideas gained through this research
experience to enhance my students’ understanding of STEM
concepts by including and adding to the current green
infrastructure on campus.
Understanding the Engineering Design Process within the Local
Watershed
Mirko I. Soko1, Ryan C. Locicero2, Dr. Maya Trotz2
1Adams
2Department
Middle School
of Civil and Environmental Engineering, University of South Florida
mirko.soko@sdhc.k12.fl.us
Keywords: STEM, Hydrologic Cycle, Watershed, Engineering Design Process
STEM (Science, Technology, Engineering and Math) education has been said to been missing
the T (Technology) and E (Engineering) parts of it; the emphasis in here will be to incorporate the E
(Engineering) within a S (Science) lesson using M (Math) principles and some T (Technology). The
Engineering Design Process (EDP), an iterative process or approach to find solutions to a broad
variety of problems, includes defining a problem based on some criteria and constraints, generating
ideas, creating a model/plan, evaluating the design and communicating the findings. Incorporating
the EDP assists with student understanding of the local watershed. Students’ prior experiences and
knowledge about the hydrologic cycle and watershed are elicited through open ended questioning.
Students participate in an inquiry activity to locate the local watershed, its boundary, the major
conveyance systems or streams, and storage systems within walking distance of the school. Next,
students survey the community to gather information regarding potential stormwater issues
(including flooding, drainage issues, water quality, and pollution concerns). The EDP will be simply
defined as ‘problem solving’ to the Middle School students. Each stormwater issue is assigned to a
group. Incorporation of the EDP promotes students to actively participate in data collection,
evaluation, planning, designing a solution of a real-world concern, and the presentation of the
findings.
22
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Nicole Richards
1. Bio Sketch
1.1.
1.2.
1.3.
1.4.
1.5.
School: C. Leon King High School
Grades Taught: 9th & 10th
Subjects Taught: Pre-IB Biology and Pre-IB Chemistry
USF Research Mentor: Ryan Locicero and Dr. Maya Trotz
Professional Goals: My long term professional goal is to obtain a
doctorate in marine biology and work in the field either as a
researcher or a professor. My short term professional goal is to
continue working as an IB teacher and to increase my laboratory
research experience. I plan to continue working with USF on
curriculum development and to engage my students
interest in science
2. Abstract
Rain Garden Performance: Vegetation Monitoring at King High School
Nicole Richards1, Ryan C. Locicero2, Dr. Maya A. Trotz2
2Civil
1C. Leon King High School
and Environmental Engineering, University of South Florida
nicole.richards@sdhc.k12.fl.us
Keywords: Florida Native Plants, stormwater, rain garden, K-12 education, STEM
Rain gardens are a type of green infrastructure used to manage stormwater at the source,
reduce nutrient inputs into large water bodies, and create multifunctional community space. Their
popularity is increasing through formal and informal education. Through the WARE RET program,
C. Leon King High School installed its first rain garden in June 2014 to help alleviate their flooding
issues. King High School students studied the role of rain gardens, designed a rain garden,
completed excavating, backfill, and planting, and chose the amounts of each of the 12 native plant
species that were planted in the rain garden.
During the 2014-2015 school year, a new group of students will monitor and maintain the
plants within the rain garden. Measurements like height, spread, number of shoots and plant mass
will be taken to assess growth. Nutrient removal from the garden will be modeled using laboratory
data on average nutrient loadings for these plants. Each student will research one of the plants in
the rain garden and create a PowerPoint about their plant with instructions on its maintenance. The
students will post their plant growth data to a wiki, a technology tool that allows real time
information sharing. Students will analyze the data collected over the year to determine the
suitability of the plants and the location of the rain garden.
23
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Stephanie Quintero
1. Bio Sketch
1.1. George Steinbrenner High School
1.2. Grades Taught: 9th - 12th
1.3. Subjects Taught: Chemistry
1.4. USF Research Mentor: Daniel Yeh and Jorge Cabria
1.5. Professional Goals: I believe in teaching students concepts, but more
importantly getting them to apply and think beyond those concepts.
High school chemistry is a very basic, yet rigorous course and many
students tend to come in with horrible preconceptions. I have conducted
action research in the past on how to raise interest and selfconceptualization in chemistry. A key finding was that by including real
world applicationsand engaging students immediately, their overall
outlook on the subject improved. My goals for WARE RET are to create
engaging classroom activities based on cutting edge research and
further develop and integrate technology into teaching chemistry.
2. Abstract
Effects on gas production and algae growth when using different
concentrations of sugar in anaerobic digesters
Stephanie Quintero1, Ken Wood2, Jorge Cabria3, Daniel Yeh3
1Steinbrenner
High School
High School
3 University of South Florida
2Wharton
stephaniequintero@live.com
kenneth.wood@sdhc.k12.fl.us
Keywords: anaerobic digester, photobioreactor, algae
The goal of this experiment is to test the effects on gas production and algae growth after
adding different concentrations of sugar to anaerobic digestors whose gas by products are being
pumped into photobioreactors. It is suspected that with greater concentrations of sugar the
anaerobic digesters will produce more gas by products (methane and carbon dioxide). This gas will
be pumped into a photobioreactor filled with algae. It is unknown if the increased amount of gas
coming from the anaerobic digesters will cause an increase or decrease in algae growth. Algae will
grow more with increased access to carbon dioxide for photosynthesis. However, an increase in
carbon dioxide will also cause changes in pH. The increased acidity may hinder algae growth. The
goal of this experiment is to help determine the parameters which encourage ideal algae growth in
photobioreactors.
24
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Tana (Jackson) Phelps
1. Bio Sketch
1.1.
1.2.
1.3.
1.4.
1.5.
School: Tampa Bay Technical High School
Grades Taught: 10th-12th
Subjects Taught: Marine Science Honors, Anatomy and
Physiology Honors, Chemistry Honors, Chemistry, Biology
USF Research Mentor: Suzie Boxman and Dr. Maya Trotz
Professional Goals: In addition to teaching my students the
curriculum standards, I hope that I effectively let them understand
how science impacts our lives. Even more specifically, I hope to
instill a sense of wonder and curiosity in my students that
translates to seeing the value of research. I will continue to run a
marine aquaponics system in my classroom. I hope to encourage
students to do research both in that system and outside of that
system with anything that interests them.
2. Abstract
Marine Aquaponics in the Classroom
Tana Jackson1, Frank Marcantoni2, Suzanne Boxman3, Dr. Maya A. Trotz3
1Tampa Bay Technical High School
2Van Buren Middle School
3Civil and Environmental Engineering, University of South Florida
tana.jackson@sdhc.k12.fl.us or tana.phelps@sdhc.k12.fl.us
Keywords: Aquaponic System, Nitrogen Cycle, Aquaculture, Hydroponics
An aquaponics system combines aquaculture (farming fish) with hydroponics (growing
plants in mineral nutrient water with no soil) so that the plants grow on the effluent from the fish
tanks. This system, that closely mimics nature, is a method to grow lean protein and fresh
vegetables. There are vast amounts of information available on fresh water aquaponics, but little on
marine aquaponics. Living in a costal environment, marine science is important to our community
and students and this research aims to integrate that through an engineered classroom model. A
larger study at the MOTE Aquaculture Research Park is underway that integrates red drum fish
with potential marketable foods in Florida, sea purslane and salt wort.
During the 2014-2015 school year my students will be use a multitrophic level aquarium
system for building and maintaining a marine aquaponics system. Initially, the students will engage
with an e-module specifically designed for this lesson. The students will be given a budget and will
select the flora and fauna based on the aquaponics design with the purpose of minimizing cost and
maximizing harvest yield. Ideally, the program will incorporate variables such as cost for the
livestock, time to harvest, nutritional requirements, mortality rate, and susceptibility to disease,
and value of the plant species. After having developed a consensus on types of living organisms and
plants, the students will create an actual marine aquaponics system with their selections. Once
created, they will monitor and measure temperature, salinity, ammonia, pH, nitrites, and nitrates
using test kits available at an aquarium store, and will adjust and maintain the system accordingly.
25
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Taryn Kendig
1. 1.Bio Sketch
2. 1.1. School: Van Buren Middle School
1.2. Grades Taught: 7th & 8th
1.3. Subjects Taught: Science
1.4. USF Research Mentor: Ryan Locicero and Dr. Maya Trotz
1.5.Professional Goals: In the last seven years I have seen the value in
utilizing authentic science research in my classroom for students of
all learning styles. With that ideal in mind, I am looking forward to
bringing STEM curriculum into the classroom at Van Buren middle school with the rain
garden project as a living lab. The truly advantageous part of this project is not only the
implications of the STEM curriculum but also that these students will be able to visibly see
the science and assess its effectiveness. My major goal with the incorporation of the rain
garden into my school community is to illustrate the scientific process for my students
using a community wide environmental problem. It is my intention to walk them through
this process start to finish so that they fully understand how the science I teach contributes
to problem solving within our community.
2. Abstract
Developing Physical Models to Analyze the Water Quality of Stormwater
within Urban Rain Gardens
Taryn Kendig1, Ryan Locicero2, Maya Trotz2
1Van
2Department
Buren Middle School
of Civil and Environmental Engineering, University of South Florida
taryn.kendig@gmail.com
Keywords: rain gardens, stormwater management, urban watersheds, filtration, low impact
development, effluent, nutrients, pollutant removal, urban runoff, water quality
One of the principle causes of a decrease in water quality to the Tampa Bay watershed is an
excess of nutrients found in stormwater. The system is being over enriched leading to increased
algae growth (eutrophication). It is the goal of the research project to educate students about the
current state of the Tampa Bay while also contributing to improving green infrastructure in their
communities. Rain gardens have been implemented into several Hillsborough County schools as a
way to manage flooding and improve water quality. This projects provides students with the
opportunity to design, build, and analyze rain gardens through integrated lessons and activities
designed by teachers. This research develops student- designed models for testing the water
quality of influent stormwater runoff. Influent stormwater will be collected during actual storm
events or spiked with known quantities of nutrients (i.e. nitrogen and phosphorus) to simulate
urban stormwater runoff. Students will use the model to analyze the stormwater as it passes
through the different designed media layers to determine nutrient removal efficiency. Based on
their experimental results they will design an optimal solution for their campus rain garden.
26
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Summer Hires
1.
Bio Sketch
1.1
1.2
1.3
1.4
School: Pre-Service at USF
Subjects Taught: Pre-Service for Secondary Ed. Biology
USF Research Mentor: Dr. Yiping Lou and Dr. Maya Trotz
Professional Goals: My goal as a future educator is to gain as much
experience as possible working in the educational field and to use what
I learn to develop innovative ways of teaching science. I plan on
creating a student-centered, diverse learning environment to help
students remain engaged and proactive in their approach to learning
scientific concepts.
2. Abstract
Documentary Film Making as a STEM Learning & Teaching Tool
Summer Hires1, Ryan C. Locicero2, Dr. Maya A. Trotz2
2Secondary
1University
of South Florida
Education for Biology, University of South Florida
ete@mail.usf.edu
Keywords: Student Engagement, Documentary Film Making , WARERET, Modeling, Engineering
Design Process
Documentaries are growing in both popularity and accessibility for today’s learner. Youtbe
and Vimeo are examples of sites that provide a platform for sharing media through public and
private channels. This type of media has the potential to present a great deal of information in an
organized and focused fashion, in a short amount of time, and in an engaging manner. They can
virtually bring together in one location diverse groups of people, geographies, and contexts thereby
making the material accessible to those without the options to have the real life experience. The
objective of this research was to develop a methodology for documenting the research activities of
RET participants and their mentors, creating short, informative multifunctional videos, and
assessing how this approach allows an educational technology professional to better grasp the
what clients are asking them to develop in terms of online learning media for K-12 students and
teachers.
The WARE RET research projects were filmed over summer 2014 and 2-5 minute
documentaries are currently under development. These videos include the learning approach used
by the teacher, interviews, and footage of both lab and field work. Six rain gardens were installed in
Tampa by RET researchers over the past year with five on three middle and high schools in the
Hillsborough County Public Schools (HCPS) district. Real time footage of construction, archival
photographs, interviews of various stakeholders (teachers, students, maintenance operators,
university researchers), and informational text were used to produce the videos which were shared
on raingardens.us. These videos tell a story about why the concept is important and applicable to
the learner. As the content producer this process offered an intensive and useful crash course in
current research trying to solve some of the global grand engineering challenges. The media
created also benefited the RET program in terms of dissemination of project information.
27
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Matt Sestilio
1. Bio Sketch
1.1. Educational Institution: University of South Florida
1.2. Subjects Taught: Pre-Service Teacher
1.3. USF Research Mentor: Ryan Locicero and Dr. Maya A. Trotz
1.4. Professional Goals: I am beginning my one-year Master’s Program at
USF this coming fall. After accomplishing this I hope to teach for at least a
few years in order to get experience under my belt. Using this experience I
would like to go back to school to do a doctoral degree. At the moment I
would like to further my studies in order to do research on curriculum
development or to become a principal.
2. Abstract
Green Infrastructure Mobile Application
Matt Sestilio, Ryan C. Locicero2, Dr. Maya A. Trotz2
2Department
of Civil and Environmental Engineering, University of South Florida
matthew15@mail.usf.edu
Keywords: Bioretention system, Rain Garden, Green Infrastructure, Geomapping, Hotspot location.
Green Infrastructure is seeing more light in the public eye with each coming year. However,
not many citizens take part in creating such structures. The purpose of this study is to create a
technological tool that allows the public to partake in green infrastructure development. The Water
Awareness Research and Education team at the University of South Florida will be working with
middle and high school students in Hillsborough County as part of a green educational experience.
Such an experience will consist of students learning about rain gardens and then using this
information to find areas that may be in need of this system. The data collected will be posted on a
Social Media platform and will consist of where the students believe a rain garden should be placed
in Hillsborough County and their reasons for it. The data collection process serves as an educational
activity for the students and a pilot test for using Social Media as a bridge between Science and the
community for green infrastructure development. As a result of this, a mobile Application will be
created on the basis that best serves the community to interact with researchers in order to create
a greener environment. Student feedback during the process will be used to create the most
optimal green infrastructure mobile application for public use.
28
RET in Engineering and Computer Science Site: Water Awareness Research and Education (WARE)
Andres Parra Garcia
1. Bio Sketch
1.1. Name of Student: Andrés Parra Garcia
1.2. Name of High School: Paul R. Wharton High School, Tampa, FL
1.3. Grade Level: 11
1.4. USF Research Mentor(s): Sarina Ergas, Ph.D.; Veronica Morales-Apontes and Adib
Amini
1.5. What have you found most valuable about this summer experience? What I found most
valuable about this experience was the expansion of previous knowledge from my past
courses at Wharton as well as gaining an improved perspective on STEM foundations as a
result of working with passionate people who work in the field and who have devoted their
education and lives to a STEM-focused career.
1.6. How do you plan to use this experience in the upcoming school year and beyond? With
the experience gained from this program, I am planning on helping, organizing and
expanding the new Biotechnology program at my school.
1.7. What are you goals for after high school? After high school I am planning to continue
my education in one of the sciences to hopefully find and grow in a STEM-focused career.
2. Abstract
Effectiveness of Biochar and N, P, K-Zeolite Fertilizer and Soil
Amendment
Andrés Parra Garcia1, Lindsay Guntner1 Veronica Apontes-Morales2, Adib
Amini2, Sarina Ergas, Ph.D.2
1Paul
2
R. Wharton High School, Tampa, Florida.
Department of Civil & Environmental Engineering, University of South Florida
lguntner@mail.usf.edu
Keywords: nutrient recovery, biochar, N, P, K,-zeolite, sustainable agriculture, anaerobic digestion
The swine industry makes up the majority of the agricultural enterprise in the United
States; nearly 40% of the world's meat production comes from this industry. As the demand
increases, concentrated animal feeding operations (CAFOs) are becoming more widespread which
leads to potential threats of the discharge of nutrient-rich wastes entering into nearby receiving
waters. As a soil amendment, biochar, a carbon-rich substance derived from biomass, has been well
studied to improve soil fertility and optimize water retention to prevent nutrient leaching. The
objective of this study is to: (1) evaluate the impact of biochar on the quality of soils after the
application of various fertilizing agents; (2) evaluate the overall productivity of the ryegrass plant;
and (3) determine the agricultural value of this proposed system. Swine manure was anaerobicallydigested to give rise to a nutrient-rich effluent where the liquid portion was sent through an ion
exchange method to generate N,P,K-Zeolite fertilizer and the biosolids were converted, through
pyrolysis, into biochar. Soil and plant tissue will be tested against a variety of parameters such as:
soil pH, nutrient leaching, soil nutrient availability as well as the plant’s growth yield. This research
will contribute to further work in developing a system that utilizes sustainable products from
wastes that will offset the need to create conventional fertilizers.
29
NSF REU in Applied Physics
30
REU: Applied Physics
Izabella Berman
1. Bio Sketch
1.1. Home Institution: Beloit College
1.2. Major: Physics
1.3. Classification: Junior
1.4. USF Research Mentor: Jagan Devkota
1.5. Professional Goals: Attain a Ph.D. in physics and apply
what I have learned toward improving the world and the
society that we live in.
2. Abstract
A new method based on RF impedance technology and soft
ferromagnetic ribbons for real-time corrosion monitoring
I. Berman1, J. Devkota2, H. Srikanth2, M. H. Phan2
1Department
2Department
of Physics, Beloit College
of Physics, University of South Florida
Izzi.berman@gmail.com
Keywords: Impedance-based sensors, Corrosive chemicals, Chemical detection, Environmental
safety
Development of a quick, cheap, and reliable technique to estimate the concentration of corrosive
chemicals has been of technological interest for safety in industries and the environment for many
years. Here we present a new approach for real-time monitoring of chemical corrosion based on the
radio-frequency (RF) impedance technology and soft ferromagnetic ribbons. The impedance (Z),
resistance (R), and reactance (X) of a commercial METGLAS® 2714A ribbon was measured in real
time for 5 μl of drop-casted HNO3 of various concentrations. Variations in the concentration of the
drop-casted acid were assessed by considering the difference (∆) in the Z, R, and X with and without
the acid treatment. The measurements performed at 0.2 MHz showed a large linear increase in the
∆Z and ∆R with the acid concentration which is ideal for developing highly sensitive chemical
sensors. Since the ribbon used is commercially available at low cost and the measurement system is
quick and low power consuming, the proposed sensor can be used as an easy, quick, and low-cost
chemical probe in industries and environmental safety purposes.
31
REU: Applied Physics
Janae Bradley
1. Bio Sketch
1.1. Home Institution: University of Missouri
1.2. Major: Biological Engineering
1.3. Classification: Senior
1.4. USF Research Mentor: Daniel Denmark and Gabriel Marcus
1.5. Profesional Goals: Pursue PhD in Biomedical
Engineering
2. Abstract
Systematic study of phase transitions in thermo-responsive polymers
used in targeted drug delivery
Janae Bradley1, Daniel Denmark2, S. Witanachchi2
1Biological
2Physics
Engineering, University of Missouri
Department, University of South Florida
Jmb64c@mail.missouri.edu
Targeted-drug delivery of blood-clotting entities and artificial blood-platelets is being considered as
a viable method to reduce the internal-wound afflicted mortality rates in battlefield soldiers. In
principle, artificial platelets, ADP and magnetic nanoparticles can be encapsulated in a polymeric
shell that is water soluble and guided to the wound site using external magnetic fields. In this
regard, the thermo-responsive polymers such as poly-N-isopropylacrylamide (PNIPAM) which can
undergo reversible phase transitions in aqueous solutions under varying temperatures, can serve
as ideal candidates for the polymer shell of the drug-capsule. Several factors such as concentration,
pH, and presence of impurity elements can affect the lower critical solution temperature (LCST) of
the PNIPAM polymer and its physical properties. In this work, we present a systematic study on the
factors that influence the LCST of the PNIPAM polymer mixed with Fe3O4 nanoparticles during
thermal bath heating. A series of PNIPAM solutions with varying concentrations of PNIPAM and
Fe3O4 nanoparticles were prepared and characterized using scanning electron microscopy and
energy dispersive x-ray spectroscopy. In-situ transmission measurements were used to determine
the LCST of PNIPAM under various aqueous concentrations and by increasing the basicity of the
PNIPAM solutions by adding adjusted KOH pellets. The controlled thermal bath heating and remote
tuning of the LCST in PNIPAM using Fe3O4 nanoparticles as reported here, provides valuable
information towards using these thermo-responsive polymers for targeted and controlled release
in drug delivery.
32
REU: Applied Physics
Jonathan D. Cuthbertson
1. Bio Sketch
1.1. Home Institution: Wheaton College, Illinois
1.2. Major: Physics
1.3. Classification: Senior
1.4. USF Research Mentor: Michael C. Cross
1.5. Professional Goals: Receive masters in biomedical physics, design
medical treatment and diagnostic equipment,
university professor/physics teacher.
2. Abstract
Surface Charge Distribution of Poly (N-isopropylacrylamide) After
Absorption of Poly-L-lysine
Jonathan Cuthbertson1, Michael C. Cross2, Dr. Garrett Matthews2
1Department
of Physics, Wheaton College, 501 College Avenue, Wheaton, IL 60187
2Department of Physics, University of South Florida
Jonathan.cuthbertson@my.wheaton.edu
Keywords: Poly(N-isopropylacrylamide), atomic force microscopy (AFM), method of image charge,
polystyrene microspheres, electrostatics, 3D tissue engineering, colloidal AFM probe, surface
charge density, cantilever resonance frequency, bioengineering, medical physics.
The goal of this experiment is to identify the interaction resulting from Poly-L-lysine (PLL)
adsorption on cell attachment on a model dynamic biomaterial, Poly (N-isopropylacrylamide)
(PNIPAM). Specifically, this paper examines how the surface charge density distributes on PNIPAM,
testing the hypothesis that it should be homogenous across the material.
Results showed that our hypothesis was correct and the surface charge density on the
PNIPAM was homogenous. This result supports the theory that cell detachment from PNIPAM, used
during 3D tissue engineering, is a strictly mechanical process rather than explained metabolically
(supported by the current literature).
33
REU: Applied Physics
Brad Davis
1. Bio Sketch
1.1. Home Institution: CUNY – Bronx Community College
1.2. Major: Engineering Science
1.3. Classification: Sophomore
1.4. USF Research Mentor: Sagar Pandit, Ph. D
1.5. Professional Goals: NASA – Aerospace Engineer
2. Abstract
Investigating the Ripple Phase in Lipid Bilayers using Molecular
Dynamics
Brad Davis1, Joseph Fogarty2, Sagar Pandit2
1Physics
and Technology Department CUNY – Bronx Community College
2Department of Physics, University of South Florida
BRAD.DAVIS@stu.bcc.cuny.edu
Keywords: Computational Physics, Biophysics, Molecular Dynamics, Soft Matter, Lipid Membrane,
Ripple Phase
The long term goal of this work is to understand the structure of ripple phase in
dipalmitoylphosphatidylcholine (DPPC) bilayer through molecular dynamics (MD) simulations and
other theoretical models. My short term goals in this project are to understand and use GROMACS
simulation tools to manipulate the MD trajectories that are already in place. Previous work by
students in CSM lab has produced long trajectories of DPPC membrane with 402400 atoms. I have
successfully manipulated the trajectories to filter in only the heavy atoms from the trajectory. The
next step is to write a C program to compute the average z-values of these heavy atoms as primary
identifiers of ripple structure. The code development and subsequent analysis is underway.
34
REU: Applied Physics
Samuel Hebenstreit
1. Bio Sketch
1.1. Home Institution: Colorado Mesa University
1.2. Major: Physics
1.3. Classification: Senior
1.4. USF Research Mentor: Nawal Khadka
1.5. Professional Goals: Attend graduate school for physics
2. Abstract
Interactions of lipid vesicles with antimicrobial peptides and drug
molecules
Samuel Hebenstreit1, Nawal Khadka2, Jianjun Pan2
1Physics,
2Department
Colorado Mesa University
of Physics, University of South Florida
shebenst@gmail.com
Keywords: lipids, lipid vesicles, antimicrobial peptides, spectral fluorometry, FTIR, calcein leakage,
POPC, POPG, tamoxifen, vitamin E
Lipids are organic molecules composed of hydrophobic fatty acid tails and hydrophilic head groups
that can form a multitude of structures. Vesicles are objects that consist of one or more lipid
bilayers. Lipid vesicles provide an excellent experimental model for cellular membranes. In this
study, we examine the effects of antimicrobial peptides and drugs on lipid vesicles. Fourier
transform infrared spectroscopy (FTIR) measurements are performed in the presence of the
antimicrobial peptide YL-75 and vitamin E. A change in absorbance corresponding to the
wavenumber regimes associated with the stretching of the carbonyl and phosphate groups is
observed. Also, a dye leakage assay is performed with POPC/POPG vesicles. Calcein dye is enclosed
within these vesicles in solution. Different concentrations of the active antimicrobial peptides YL75, YL-77, the inactive antimicrobial peptides YL-65, YL-60, and tamoxifen are incubated with the
vesicles. A time-course measurement of incubation allows for determination of maximum leakage.
Percent leakage of samples incubated for one to two days is determined by measuring the
fluorescence before and after breaking the vesicles with Triton X-100 detergent (to normalize).
Different leakages are observed for active vs. inactive peptides.
35
REU: Applied Physics
Luis Martinez
1. Bio Sketch
1.1 Home Institution: University of Puerto Rico-Humacao
1.2 Major: Physics
1.3 Classification: Junior
1.4 USF Research Mentor: Kristen Stojak, Zohreh Nemati
1.5 Professional Goals: Acquire a PhD in Physics. Become a professor
and researcher
1. Abstract
Study of octopod shaped nanoparticles and their bio-medical
applications
Luis Martinez1, Zohreh Nemati2, Kristen Stojak Repa2, Hafsa Khurshid2, Javier
Alonso2, Manh-Huong Phan2, Hariharan Srikanth2
1Physics
2 Physics
Department, UPRH
Department, University of South Florida
luis.martinez30@upr.edu
Keywords: Magnetic
Decomposition
Hyperthermia,
Functionalized
magnetic
nanoparticles,
Thermal
Among biomedical applications of nanoparticles (nps), magnetic hyperthermia (using
magnetic nps to heat and destroy cancer cells) is one of the most promising. Recent studies have
shown that the performance of these nps can be improved by tuning their aspect ratio, which
allows us to increase their surface anisotropy in order to attain optimal heating power. In this
study, we prepare octopod shaped iron oxide nps of various sizes in order to improve their heating
efficiency. For the synthesis, we rely on thermal decomposition of the organic precursors iron (III)
acetylacetonate, oleylamine and oleic acid. By changing the relative proportion of these precursors,
we obtain nps of various shape and size. Once nps are synthesized, they are functionalized with
TMAH to become water soluble. Their structural properties were analyzed using a combination of
XRD and TEM, revealing a predominant Fe3O4 iron oxide phase, with high crystallinity and narrow
size distribution. Magnetic properties were studied both as a function of the temperature and the
magnetic field, by using a PPMS magnetometer. Results suggest an improved magnetic response of
the nps with increasing size. Finally, hyperthermia experiments were carried out using an Easyheat
LI 3542 system, by applying different magnetic fields (up to 800 Oe) at a constant frequency of 310
kHz.
36
REU: Applied Physics
Carl McIntyre
1. Bio Sketch
1.1. Home Institution: Colorado Mesa University
1.2. Major: Physics
1.3. Classification: Senior
1.4. USF Research Mentor: Jagannath Paul
1.5. Professional Goals: Materials Physics R&D
2. Abstract
A Study of Two Dimensional Electron Gas Using 2D Fourier Transform
Spectroscopy
C. McIntyre1, J. Paul2, D. Karaiskaj2
1Department
of physics, Colorado Mesa University, 1100 North Avenue, Grand Junction, Colorado
81501
2Department of physics, University of South Florida, 4202 East Fowler Ave., Tampa, Florida 33620
cmcintyr@mavs.coloradomesa.edu
Time integrated four wave mixing and two dimensional Fourier transform (2DFT)
spectroscopy were used to study the dephasing of the Fermi edge singularity (FES) in a
symmetrically modulation doped 12 nm single quantum well GaAs/AlGaAs system. We chose a 797
nm wavelength laser pulse split through four nested and folded Michelson interferometers to
ensure phase stabilization. Three pulse four wave mixing using S1 rephasing pulse sequence was
incident on the sample within a cryostat at 5 K. Temperature was increased from 5K to 30K and a
linear homogeneous linewidth broadening of the 2DFT spectra was detected.
37
REU: Applied Physics
Boeing Smith
1. Bio Sketch
1.1. Home Institution: Wittenberg University
1.2. Major: Physics
1.3. Classification: Sophomore
1.4. USF Research Mentor: Corisa Kons
1.5. Professional Goals: Pursue Materials Engineering PHD.
2. Abstract
Band Energy Modification of Ferroelectric ZnSnO3 for Photovoltaic
Applications
Boeing Smith1, Corisa Kons2, Anuja Datta2
1Wittenberg
University Department of Physics
Cluster for Advance Smart Sensor Technologies and
University of South Florida Department of Physics
2 Florida
smithm4@wittenberg.edu
Keywords: Doping, ferroelectric, band gap, photovoltaic
LiNbO3 (LN) type ZnSnO3 is a non-toxic and inexpensive material that has been gaining interest as a
potential absorber material for next generation FE based solar cells. This material exhibits high
remnant polarization (~60 µC/cm2) along the c-axis and is a suitable non-toxic replacement of Cdand Pb-based absorber materials in conventional solar cells. However, ZnSnO3 has a
relatively large band gap (~3.8 eV) and needs judicious engineering of band-structure to
harvest broader spectrum of sunlight. We have earlier synthesized ZnSnO3 FE nanowire arrays
showing high polarization properties. In this work, we demonstrate that through site-specific cation
(Sb, Cu, Ca, Ba) doping, it is possible to tune the band gap of the ZnSnO 3 due to the
strong correlation between band gap and the lattice constant in this material. We synthesized
the doped and undoped ZnSnO3 as nanorods (~7-10 nm in diameter) using a one-step, low
temperature solvothermal process. The structural and chemical analyses performed by X-Ray
Diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM),
and energy dispersive spectroscopy (EDS) confirmed the phase, nanostructure, and
successful doping in the nanorods. The optical properties of the doped ZnSnO3 was assessed
by Raman Spectrometer and UV-Vis Spectrometer from which the band gap was obtained from the
latter by using Absorption Spectrum Fitting. Electrical and photoconductivity analyzes were also
obtained to better understand the efficacy of using the FE doped ZnSnO3 nanorods as a future solar
cell material.
38
REU: Applied Physics
Kim Sonju
1. Bio Sketch
1.1. Home Institution: California State University San Bernadino
1.2. Major: Applied Physics
1.3. Classification (Freshman, Sophomore, Junior, Senior): Senior
1.4. USF Research Mentor: Sinhara Rishi Silva
1.5. Professional Goals: Research.
2. Abstract
Second Harmonic Generation in Non-linear Metamaterials
Kim Sonju1, Sinhara Rishi Silva2, Dr. Jiangfeng Zhou2
1Department
2Department
of Physics CSU San Bernardino
of Applied Physics, University of South Florida
kjsonju@hotmail.com
Keywords: metamaterial, non-linear, phase matching, second harmonic enhancement, second
harmonic generation, split ring resonator, varactor diode
We demonstrate a nonlinear metamaterial which produces higher order harmonics in the
microwave frequency regime. Nonlinearity in the structure is introduced by adding a
varactor diode in the common slit of the double split ring resonator (DSRR) design. By
engineering the structure such that inner ring resonance frequency of the DSRR is twice the
outer ring resonance frequency, we have demonstrated that the second harmonic of the
outer ring can be enhanced significantly for a single unit cell. Furthermore, the second
harmonic of the periodic arrays of the unit cells can be improved by carefully positioning
the arrays such that phase matching condition is satisfied. When the phase matching
condition occurs the enhancement is greater than a single ring array. In addition, with the
enhancement of the second harmonic, other higher order harmonics also can be enhanced.
39
REU: Applied Physics
Otho Ulrich
1. Bio Sketch
1.1. Home Institution: Western Michigan University
1.2. Major: Physics
1.3. Classification: Junior
1.4. USF Research Mentor: Joseph Gonzalez
1.5. Professional Goals: Computational Physics, Astrophysics
2. Abstract
Molecular Dynamics of Bicrystalline Graphene to Determine Fracture
Strength
Otho Ulrich1, Joseph Gonzalez2, Kien Cong Nguyen2, Ivan Oleynik2
1Western
2University
Michigan University Department of Physics
of South Florida Department of Applied Physics
otho.a.ulrich@wmich.edu
Keywords: Materials Science, Computational Physics, Molecular Dynamics, Graphene
In its pristine form, graphene is one of the strongest materials measured, and possesses a wide
range of technologically appealing characteristics. Several recent experiments have explored the
mechanical properties of graphene, composed of grains grains, which seem to show contradictory
results. In this work, we utilize classical molecular dynamics to investigate the response of
bicrystalline graphene subjected to uniaxial and biaxial stress. Atomic-scale modeling of the
material elucidate trends in the strength of the material as a function of misorientation angle and
provide clues into the mechanism responsible for the strengthening of the grain boundaries. We
also show the validity of the results is highly dependent on the potential chosen to describe the
carbon-carbon interactions. This is achieved by comparing the three most commonly used
potentials with our own potential developed previously.
40
NSF REU: Chemistry Summer Enhanced
Experience Discovering Science
41
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Christopher Brewer
1. Bio Sketch
1.1. Home Institution: Florida State University
1.2. Major: Chemistry & Biochemistry
1.3. Classification: Senior
1.4. USF Faculty Mentor: Dr. Kirpal Bisht
USF Graduate Mentor: Ali Husain
1.5. Professional Goals: To pursue a graduate degree in
Chemistry.
2. Abstract
Design and the Synthesis of Novel Water-Soluble Resorcin[4]arenes
Decorated with Maltose monomers via Cu(I)-catalyzed Azide-Alkyne
Cycloaddition (CuAAC)
Christopher Brewer1, Ali Husain2, Kirpal Bisht2
1Department
of Chemistry & Biochemisty, Florida State University
of Chemistry, University of South Florida
2Department
crb10j@my.fsu.edu
Keywords: Resorcin[4]arene, CuAAC, green chemistry, catalyst
Water is the most economically and environmentally friendly solvent and has many advantages
over organic solvents which are toxic, volatile and expensive. Water is generally cheap, reusable,
non-toxic, abundant, environmental and eco-friendly. Thus, replacing organic solvents with an ideal
solvent such as water attracts considerable attention and has remarkable impact in organic
syntheses due to increases in environmental regulations. Resorcin[4]arenes are water-insoluble
macrocylic tetramers that consist of four resorcinol units providing a high electron-rich cavity
system, with multiple phenolic groups (upper rim) and hydrophobic lower region (lower rim). This
type of macromolecue can be easily modified and decorated with different functionalities in gram
quantity. Novel water-soluble Resorcin[4]arenes were designed and synthesized by decorating the
upper rims of synthesized resorcin[4]arene intermediates with maltose monomers via Cu(I)catalyzed Azide-Alkyne Cycloaddition (CuAAC). The presence of the moltose monomers and varying
the flexibility on the resorcin[4]arene scaffold, gives these maltose moieties the capability to act as
a larger, wider, and deeper type of cavity. Therefore, the utility of these macromolecules being
soluble in aqueous media and having the ability to act as host-guest molecules were investigated as
catalysts for green-organic transformations in water.
42
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Veronika Espinoza
1. Bio Sketch
1.1. Home Institution: California State University of San Marcos
1.2. Major: Chemistry
1.3. Classification: Senior Undergraduate
1.4. USF Faculty Mentor: Dr. Harmon
USF Graduate Mentor: Tamalia Julien
1.5. Professional Goals: Obtain a Ph. D. in Nueroscience
2. Abstract
Synthesis and Characterization of Self-Healing Polycarbonate
Polyurethane-Copper-based Paddle Wheels
Veronika Espinoza1, Tamalia Julien2, Dr. J.P. Harmon2
1California
2Department
State University of San Marcos, San Marcos, CA
of Chemistry, University of South Florida, Tampa, FL
espin059@cougars.csusm.edu
Keywords: Polycarbonate Polyurethane, Copper-based paddle wheel, hydrogen bonding, selfhealing
Polycarbonate Polyurethanes (PCPU) are a family of polymers which are often used in wound
dressings and balloon catheters. The ultra-soft PCPU formulation used in this project has the ability
to intrinsically repair itself after damage occurs. Hydrogen bonding within the PCPU is thought to
be primarily responsible for the mechanical characteristics and the manifestation of the observed
self-healing properties. It has been shown that copper-based paddle wheel secondary building units
have multiple hydrogen bonding sites which the polar PCPU can interact with. The research
undertaken includes adding different composite weight percentages of the copper-based paddle
wheel [Cu2(p-OH benzoate)4(DMSO)2]•2DMSO with the PCPU. The aim of this project is to increase
the neat polymer’s self-healing ability by increasing hydrogen bonding within the sample. Different
techniques such as Differential Scanning Calorimetry, Tensile Testing, FT-IR Spectroscopy, and
healing analysis will be used. These techniques are used to analyze the composites’ thermal,
mechanical, and optical properties, how they relate and change with composition, and how they
affect the self-healing ability of the neat polymer.
43
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Arielis Estevez
1. Bio Sketch
1.1. Home Institution: University of Puerto Rico, Rio Piedras Campus
1.2. Major: Chemistry
1.3. Classification: Junior
1.4. USF Faculty Mentor: Dr. Jon C. Antilla
USF Graduate Mentor: Susana S. López
1.5. Professional Goals: My future goals are to pursue a BS in chemistry and
get a Ph.D. in pharmacology, do research and work in the industry.
2. Abstract
Methodology for an Enantioselective Brønsted Acid-catalyzed Reductive
Aldol of α,β-Unsaturated Ketones
Arielis Estevez1, Susana S. López2, Jon C. Antilla2
1Chemistry
Department, University of Puerto Rico, Rio Piedras Campus
Department, University of South Florida
2 Chemistry
sileira1@gmail.com
Keywords: Asymmetric synthesis, Aldol reaction, organocatalysis, 1,4 conjugate addition, α, βunsaturated enones
Due to the chiral nature of many biologically active molecules, developing and improving
methodologies that generate stereogenic carbon centers is crucial as enantiomers of compounds
exhibit different chemical reactivity. Asymmetric reactions able to form carbon-carbon bonds are
important transformations in organic synthesis. In the literature are well-known methodologies
that use organometallic catalysts to produce such bonds. In recent years, the use of organocatalysts
has moved to the forefront of asymmetric synthesis owing in part to the ease of use and low cost of
organocatalysts. BINOL-derived chiral phosphoric acid catalysts have proven effective in many
reactions previously performed with organometallic catalysts. Modifications at the 3, 3’-positions
allows these catalysts to be versatile and fine-tuned for specific reactions. This work proposes the
development of a one-pot asymmetric reductive-Aldol reaction with α, β-unsaturated enones and
various electrophiles using a BINOL-derived chiral phosphoric acid.
44
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Nelson Garcia-Vazquez
1. Bio Sketch
1.1. Home Institution: University of Puerto Rico Rio Piedras Campus
1.2. Major: Chemistry
1.3. Classification: Junior
1.4. USF Faculty Mentor: Dr. Peter Zhang
USF Graduate Mentor: Dr. Li-mei Jin
1.5. Profesional Goals: My profesional goals include to complete
Ph.D. in Organic Chemistry, and work in both research and
industry.
2. Abstract
D2-symmetric porphyrin synthesis and its application in the
cyclopropanation of α,α-diphenylethylene
Nelson Garcia-Vazquez1, Dr. Li-Mei Jin2, Dr. Peter Zhang2
1Chemistry
Department, University of Puerto Rico Rio Piedras Campus
Department, University of South Florida
2Chemistry
gvnelson1@gmail.com
Keywords: Cyclopropanes, Cobalt(II) Porphyrin, Metalloradical Catalysis
Cyclopropanes are important in synthesis due to their ability to be employed when constructing
complex compounds, including bioactive molecules. Cyclopropanation of olefins leads to the
synthesis of cyclopropanes with varied functionalities. Such cyclizations have been achieved with
Cobalt(II) complexes of D2-symmetric porphyrins, in high product yield, diasterometric ratio (dr),
and enantiomeric excess percent (ee%) through a radical mechanism. MRC catalysts such as the
[Co(3,5-DitBu-ChenPhyrin)] and [Co(3,5-DitBu-QingPhyrin)] have been tested in a variety of
carbene and nitrene transfer reactions. Subtle differences in structure lead to different outcomes in
terms of certain reactions. For example, in an recently published intramolecular cyclopropanation,
[Co(3,5-DitBu-QingPhyrin)] gave 96 % ee while only 56% ee was observed when using [Co(3,5DitBu-ChenPhyrin)] as the catalyst. Thus, this project intends to synthesize a new generation of
porphyrin catalyst that includes a diphenylcyclopropyl unit. The reactivity and selectivity of this
new generation of porphyrins will be tested in MRC catalytic system.
45
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Jermaine Henderson
1. Bio Sketch
1.1. Home Institution: University of Massachusetts Boston
1.2. Major: Chemistry
1.3. Classification: Senior
1.4. USF Faculty Mentor: Abdul Malik, Ph.D.
USF Graduate Mentor: Sheshanka Kesani
1.5. Professional Goals: To be the first in my family to graduate from
college and attend graduate school.
2. Abstract
Sol-gel Niobia based sorbent in CME coupled to HPLC for enriching
organophosphorus pesticides
Jermaine Henderson1, Sheshanka Kesani2, Abdul Malik2
1University
2Department
of Massachusetts Boston, Boston, MA
of Chemistry, University of South Florida, Tampa, FL
Jermaine.Henderso001@umb.edu
Keywords: Organophosphorus pesticides, capillary micro-extraction, sol-gel based sorbents
Organophosphorus pesticides (OPs) can be hazardous to the environment as well as human beings
due to their inhibition of the enzyme acetylcholinesterase (AChE). Techniques such as liquid-liquid
extraction and solid phase extraction have been used in enriching OPs yet solid phase microextraction (SPME) offers a solvent-free alternative. One objective of this research is to develop an
extraction technique using capillary micro-extraction (CME) coupled to high performance liquid
chromatography (HPLC) to preconcentrate and analyze OPs. Another objective is to develop a solgel based niobia-polyethylene glycol sorbent for CME to extract OPs. The extraction performance of
sol-gel based niobia sorbent will be compared with titania and silica. As a preliminary step, three
polar analytes were extracted by CME and analyzed by HPLC. Extraction performance of the sol-gel
niobia based sorbent with these polar analytes has proven sol-gel CME-HPLC to be a promising
technique for extraction and analysis of OPs.
46
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Reed Jacobsen
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Biochemistry
1.3. Classification: Senior
1.4. USF Faculty Mentor: Dr. Kirpal Bisht
USF Graduate Mentor: Ali Husain
1.5 Professional Goals: Graduate School
2. Abstract
Synthesis of Novel Water-Soluble Recorcin[4]Arene Cavities Decorated
with Sugar Monomers via CuAAC Cyclo- Addition Allow for Chemical
Synthesis in an Aqueous Environment
Reed Jacobsen, Ali Husain, Dr. Kirpal Bisht
Department of Chemistry University of South Florida
Reed15@mail.usf.edu
Keywords: Green Chemistry, Catalyst, Water-Soluble, Recorcin[4]Arene
The ever present need for environmentally friendly chemical synthesis is a major concern in the
scientific community today. By eliminating the need for organic solvent systems in chemical
reactions, a number of green chemistry processes can be employed. Through click chemistry
recorcin[4]arenes was designed and synthesized with sugar monomers via 1,4-distributed 1,2,3triazoles and characterized by nuclear magnetic resonance (NMR) spectroscopy. By shielding the
electron rich hydrophobic cavity system of recorcin[4]arenes with polar sugar moieties, the utility
of the cavity system can be employed in an aqueous medium. These novel compounds allow
chemical synthesis to be achieved in high yields in an aqueous environment by acting as a hostguest molecule, in which reactions can take place. Their ability to catalyze organic reactions in
water may play an important role to green chemistry, as environmental concerns with present
synthetic procedures and is an ever-growing problem.
47
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Josue Liriano
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Biomedical Sciences
1.3. Classification: Senior
1.4. USF Faculty Mentor: Dr. Jon C. Antilla
USF Graduate Mentor: Susana S. Lopez
1.5. Professional Goals: Graduate school program in chemical
biology followed by a postdoc.
2. Abstract
CHIRAL PHOSPHORIC ACID-CATALYZED
FRIEDEL-CRAFTS ALKYLATION OF INDOLES AT THE 2-POSITION
Josue Liriano, Susana S. Lopez, Jon Antilla*
Department of Chemistry at the University of South Florida
lirianoj@mail.usf.edu
Keywords: organocatalysis, friedel-crafts, indole, binol, phosphoric acid
Indoles are of great interest to synthetic organic chemists due to the significant number of
biologically active natural products, which contain this scaffold. From the medicinal
chemistry perspective, these privileged structures represent an important component in
many approved pharmaceuticals used as therapeutic agents. Substituted indoles, especially
those substituted at the 2-position, are latent intermediates for numerous alkaloids and
pharmacologically important substances. Alkylation methods using the well-known FriedelCrafts reaction have been of significant interest in recent years. Developing enantioselective
methodologies which can perform these transformations using organocatalysts can expand
the already important synthetic utility of indoles. The work presented herein describes the
development of a methodology for an enantioselective Friedel-Crafts alkylation reaction of
indoles at the 2-position by a BINOL-derived phosphoric acid.
48
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Nathan Pickering
1. Bio Sketch
1.1 Home Institution: Northwest Missouri State University
1.2 Major: Chemistry (ACS Accredited)
1.3 Classification: Senior
1.4 USF Faculty Mentor: Arjan van der Vaart, Ph.D.
USF Graduate Mentor: Geoffrey Gray
1.5 Professional Goals: After I receive my B.S. in chemistry I plan to
attend graduate school to obtain an advanced degree in chemistry
with emphasis in renewable energy.
2. Abstract
Identification of Structural Changes Caused by the N7-methylguanine
Damage in DNA
Nathan Pickering1, Geoffrey M. Gray2, Arjan van der Vaart2
1Northwest
2Department
Missouri State University, Maryville, MO
of Chemistry, University of South Florida, Tampa, FL
nathan.t.pickering@gmail.com
Keywords: DNA damage recognition, molecular dynamics, indirect readout, free energy
Damaged DNA can cause toxicity or cancer, which causes almost 15% of all human deaths. Proteins
are able to detect damage through changes in properties of the DNA. Indirect readout, or
recognition of damaged DNA through a physical property, is a mechanism of damage recognition.
Recognition of N7-methylguanine (7-mG) is believed to occur, at least in part, by an indirect
readout mechanism. Previous work evaluating the effects of 7-mG has found that this form of
damage results in a more thermodynamically stable structure, along with potential conformational
changes in DNA structure. The purpose of this work is to find a difference between the healthy and
7-mG damaged DNA strands to better understand protein-DNA recognition. To assess the possible
effects of the 7-mG on the structure of DNA, molecular dynamics (MD) simulations were performed
on the Dickerson-Drew dodecamer (5'-CGCGAATTCGCG-3') and well as a damaged version of the
sequence (5'CGC(7-mG)AATTCGCG). This study will further the understanding of this type of DNA
damage recognition by proteins.
49
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Santiago Rodriguez-Ospina
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Microbiology and Biochemistry
1.3. Classification: Senior
1.4. USF Faculty Mentor: Dr. David J. Merkler
USF Graduate Mentor: Daniel R. Dempsey
1.5. Professional Goals: Pursuing the medical-research field
2. Abstract
Identification and Characterization of Drosophila Melanogaster
Polyamine N-acyltransferase
Santiago Rodriguez-Ospina, Daniel R. Dempsey, and David J. Merkler
Department of Chemistry, University of South Florida
santiago3@mail.usf.edu
Keywords: Polyamine, N-acyltransferase, Drosophila melanogaster, fatty acid amides.
Fatty acid amides are a prominent family of lipids engaged in the inactivation of
neurotransmitters, as cell signaling lipids, in the biosynthesis of melatonin, and insect cuticle
sclerotization. These fatty acid amides are composed of long and short-chain N-acyl amino acids, Nacylethanolamines, N-arylalkylamides, and the N-monoacylpolyamides. One biosynthetic step in the
degradation of the polyamines is the enzyme-catalyzed formation of N-monoacylpolyamines, which
is catalyzed by a polyamine N-acyltransferase (PNAT). PNAT from Drosophila melanogaster a
member of the Gcn5-related N-acetyltransferases family was expressed and shown to catalyze the
formation of N-acylpolyamines from the corresponding acyl-CoA and polyamine. The enzyme was
evaluated using pH rate profiling, which yielded data suggesting an acid/base chemical mechanism,
followed by site-directed mutagenesis of residues proposed to be involved in catalysis. Herein, we
report data that has led to a proposed chemical mechanism for D. melanogaster PNAT.
50
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Vinushka Schalk
1. Bio Sketch
1.1. Home Institution: New College of Florida
1.2. Major: Chemistry/Computer Science
1.3. Classification: Second Year
1.4. USF Faculty Mentor: Dr. H. Lee Woodcock, III
USF Graduate Mentor: Yuri Pevzner
1.5. Professional Goals: To become a computer programmer in the
chemical industry, involved in algorithm development
2. Abstract
Development of CHARMMing as a graphical Web user interface for
generalized multi-scale modeling
Vinushka Schalk1, Yuri Pevzner2, Dr. H. Lee Woodcock, III2
1Department
of Natural Sciences, New College of Florida
of Chemistry, USF-Tampa
2Department
stefan.schalk12@ncf.edu
Keywords: multiscale modeling, QM/MM, Q-Chem, CHARMM, education
Computation has become an essential part of chemical research. However, computational chemistry
software tends to have fairly steep learning curves. Thus, graphical user interfaces have been
developed to simplify the setup, execution, and analysis of chemical and biological calculations. The
CHARMMing (CHARMM interface and graphics) Web portal is one such graphical interface,
developed for the widely used molecular mechanics simulation package CHARMM (Chemistry at
HARvard Macromolecular Mechanics). This work reports the implementation of a generic
framework for managing multiscale simulations using various QM (quantum mechanical) and MM
(molecular mechanics)-specific programs, e.g., Q-Chem. A set of interactive tutorials called “lessons”
have been developed to introduce new users to these software packages. Further, a framework for
rapidly developing new tutorials has been implemented. This Web interface will be a useful tool for
advanced undergraduate and new graduate students as well as novice computational researchers,
facilitating the set up of their own simulations.
51
Chemistry Summer Enhanced Experience Discovering Science: Chem-SEEDS
Anne-Genevieve Telcy
1. Bio Sketch
1.1. Home Institution: Florida Southern College, Lakeland, Fl
1.2. Major: Chemistry
1.3. Classification: Undergraduate
1.4. USF Faculty Mentor: Dr. Sheryl Li
USF Graduate Mentor: Sung Yub Han
1.5 Professional Goals: My professional goals are to get a Ph. D in
forensic chemistry and hope to work for NASA.
2. Abstract
Optimization of silver shell and silica core nanoparticles as SERS active
substrate
Anne-Genevieve Telcy1, Sung Yub Han2, Dr. Sheryl Li2
1Florida
2Chemistry
Southern College, Lakeland, FL
department, University of South Florida, Tampa FL
telcyanne@gmail.com
Keywords: Surface-Enhanced Spectroscopy (SERS), Scanning Electron Microscopic (SEM)
Silver nanoparticles improve sensitivity in Surface-Enhanced Raman Spectroscopy (SERS).
However, these nanoparticles have limitations due to the wavelength they absorb when applied in
biological SERS analysis. To address this problem, silica-core nanoparticles have been proposed to
help silver nanoparticles absorb greater wavelength. This project intends to synthesize
nanoparticles that have a silica core and a silver shell, and this process involves three steps: (1)
synthesis of the silica core, (2) attachment of silver seeds to the surface of the silica core, and (3)
growth of the silver shell on the silica core. The size and the shape of the nanoparticles will be
analyzed using scanning electron microscopy (SEM) and UV spectroscopy in order to determine the
optimum dimensions. The preparation and the characterization of core and shell nanoparticles
were determined as a result of this experiment.
52
NSF REU in Ubiquitous Sensing
53
REU: Ubiquitous Sensing
Alejandra Vidal
1. Bio Sketch
1.1 Home Institution: Universidad del Turabo
1.2 Major: Computer Engineering
1.3 Classification: Senior
1.4 USF Research Mentor: Juan José Marrón
1.5 Professional Goals: Graduate on December 2014
2. Abstract
Real-time Pedestrian Tracking in Indoor Environments
Alejandra Vidal Ortiz1, Juan José Marrón2, Miguel A. Labrador2
1Department
2Department
of Electrical and Computer Engineering, Universidad del Turabo
of Computer Science and Engineering, University of South Florida
vidal4275@gmail.com
Keywords: Smartphones, Indoor positioning, Pervasive computing, Location-based information
systems
Tracking pedestrians in indoor environments has increasingly become a useful tool to optimize
human activities and it is applicable in multiple scenarios such as emergency interventions,
guidance entertainment, advertising and healthcare. In addition, the ubiquitous/pervasive,
technical and sensing capabilities of portable devices have become them an attractive option for
indoor pedestrian tracking based on dead-reckoning (i.e., the identification of human gait for step
detection and distance estimation). This research presents a system for real-time ubiquitous
tracking of pedestrian in indoor environments, using sensing raw data from low cost sensors (e.g.,
accelerometer and gyroscope) embedded in modern smartphones. The proposed system combines
a simplified dead reckoning approach along with the detection of identifiable indoor signatures
such as turns. The system was implemented and tested on Android-based mobile phones obtaining
an accuracy of 89.93% in common indoor human motion displacements.
54
REU: Ubiquitous Sensing
Edwin Miguel Peguero
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Mathematics and Computer Science
1.3. Classification: Senior
1.4. USF Research Mentor: Miguel A. Labrador, Ph.D.
1.5. Professional Goals: Researcher
2. Abstract
Correlation-based Rotation-invariant Biometric Gait Recognition System
Using On-board Sensors on Android Mobile Phones
Edwin Miguel Peguero, Miguel A. Labrador
Department of Computer Science and Engineering, University of South Florida
edwinmpeguero@gmail.com
Keywords: Biometric system, gait, cycle, accelerometer, gyroscope, autocorrelation
Biometric recognition systems are receiving growing interest as security concerns increase across
various different domains. They offer increased protection compared to knowledge-based or tokenbased security systems that are vulnerable to identification theft, sharing, or loss. As of today, no
biometric security solution is widely available in the domain of mobile phone, despite the large
security improvements they offer. This research presents a biometric gait recognition system that
can provide security to the user without the need for user interaction. This is accomplished through
the continuous collection and evaluation of accelerometer and gyroscope data. We expect to entice
mobile phone users who would rather not deal with password security methods to adopt the
security method. In addition, such a biometric system allows for more robust security in the form of
continuous person recognition.
55
REU: Ubiquitous Sensing
Luis N. Valcourt
1. Bio Sketch
1.1. Home Institution: University of Puerto Rico at Bayamon
1.2. Major: Computer Science
1.3. Classification: Senior
1.4. USF Research Mentor: Yueng De la Hoz
1.5. Professional Goals: Professor
2. Abstract
Human Fall Detection with Smartphones
Luis N. Valcourt1, Yueng De La Hoz2, Miguel A. Labrador2
1Department
2Department
of Computer Science, University of Puerto Rico
of Computer Science and Engineering, University of South Florida
luisvalcourt2@gmail.com
Keywords: Fall detection, smartphone, gyroscope, accelerometer
According to the Centers for Disease Control and Prevention (CDC), one in three people over the age
of 65 experience a fall. From twenty to thirty percent of these people sustain injuries such as
fractures, loss of independence, and even death. Fall detection is an active research area that strives
to improve people’s lives using pervasive computing. This research presents a fall detection system
based on data gathered from a smartphone. It utilizes the smartphone’s built-in sensors
(accelerometer, gyroscope) to identify the location of the cellphone on the user’s body (e.g., chest,
pocket and holster) as well as to find known patterns associated with falls. A general description on
fall detection systems is provided, including the different types of sensors used nowadays. The
proposed solution is presented and described in great detail. Finally, the system is assessed using
known performance indicators.
56
REU: Ubiquitous Sensing
Mark Di Sano
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Computer Engineering
1.3. Classification: Senior
1.4. USF Research Mentor: Miguel A. Labrador, Ph.D.
1.5. Professional Goals: Research additional use cases of commonly
available tech devices such as cell phones and cameras.
2. Abstract
HeartMApp: An Android Application to Reduce Patient Readmission Rate
and Health Risk after a Cardiovascular Event
Mark Di Sano, Miguel A. Labrador
Department of Computer Science and Engineering, University of South Florida
markdisano@gmail.com
Keywords: Android application, peak detection, zephyr bioharness, cardiovascular disease
Although there have been specific risk factors identified with Cardiovascular Diseases (CVD), not all
of them can be changed during a brief medical visit. HeartMApp provides a multi-component
approach to address this issue. The first component is the ‘Assessment’ part, where patients are
prompted with a color coded condition that best closely matches the answers to various risk factor
questions. The second component is the ‘CHF Info’, which provides information regarding different
topic questions about Cardiovascular Heart Failure (CHF). The third component is the ‘Vital Signs’
section, where the patient receives a near live view of their heart rate and heart rate variability. The
last component is the ‘Exercise’ section, which has two exercises commonly recommended to
patients. The system is designed to transmit the patient’s data from the Bioharness3 strap to the
server whenever the patient is running the Assessment, Vital Signs, or Exercise components.
57
REU: Ubiquitous Sensing
Steven Díaz Hernández
1. Bio Sketch
1.1. Home Institution: Universidad del Turabo
1.2. Major: Computer Engineering
1.3. Classification: Senior
1.4. USF Research Mentor: Yueng De la Hoz
1.5. Professional Goals: Graduate school
2. Abstract
Dynamic Background Subtraction for Fall Detection System using a 2D
Camera
Steven Díaz Hernández1, Yueng De La Hoz2, Miguel Labrador2
1Department
2Department
of Electrical and Computer Engineering, Universidad del Turabo
of Computer Science and Engineering, University of South Florida
stevendiazhernandez@gmail.com
Keywords: Fall detection, background subtraction, computer vision, movement detection
Falls are the number one cause of fractures, trauma, loss of independence and deaths among older
adults and each year more than 1.6 million older U.S. adults go to emergency departments
according to National Institutes of Health (NIH). Therefore, it is necessary to provide different types
of mechanisms to detect people from falling in order to improve their lives. This project presents a
fall detection system that utilizes a 2D camera. It employs an open source libraries (OpenCV) and
computer vision techniques in order to detect falls. Background subtraction (dynamic) and
movement detection are used for fall detection as well as flags and timers to improve the accuracy
of the system. The flags are used to avoid false positives when the starting position is the floor.
Timers determine the minimum amount of time a person should lie on the floor in order to detect a
fall. Finally, the system was implemented and evaluated obtaining an accuracy of 85.37% of fall
detection.
58
NSF REU: Tampa Interdisciplinary Environmental
Research
59
REU: Tampa Interdisciplinary Environmental Research (TIER)
Allison Wood
1. Bio Sketch
1.1. Home Institution: The University of New Hampshire
1.2. Major: Environmental Engineering
1.3. Classification: Junior
1.4. USF Research Mentor: Eunyoung Lee
1.5. Professional Goals: Work with the EPA to develop
responsible, sustainable usage plans for water resources in
the US
2. Abstract
Kinetics of the Anaerobic Co-Digestion of Algae and Waste Activated
Sludge
Allison Wood1, Eunyoung Lee2, Qiong Zhang2
1Department
2Department
of Environmental Engineering, University of New Hampshire
of Civil & Environmental Engineering, University of South Florida
ark96@wildcats.unh.edu
Keywords: Anaerobic Digestion, Co-Digestion, Algae, Kinetics
Global energy consumption is on the rise, and the continued use of fossil fuels to meet this
demand is a dangerous choice for the environment. In the search for alternative energy, biofuels
hold great potential as renewable sources that could impact energy production on a large-scale.
Biofuels derived from food crops have made significant commercial progress, however the land
necessary for large-scale implementation is presently in use for agriculture. Biodiesel production
using algae has also received attention, however with current technologies the process is
unsustainable. Algae still holds great potential in the realm of renewable energy- the current idea is
to co-digest it with various wastes under wet, anaerobic conditions in order to produce biogas.
Many anaerobic digestion studies involving processing wastes are present in the literature, but
microalgal co-digestion studies are limited- certain studies have utilized waste paper and manures,
but no research has thoroughly investigated the interaction of metabolic processes related to the
combination of algae and municipal waste sludge. As anaerobic digesters are fairly common in
wastewater treatment plants, the optimization of such a co-digestion process would be significant
to the future of both wastewater treatment and renewable energy production. The goal of this
study was to obtain experimental kinetic information and to create a model that would enhance
general understanding of this co-digestion process, leading to optimization of the metabolic
processes involved. Experimental data was collected over the course of 25 days from lab-scale
batch reactors operating under mesophilic conditions.
60
REU: Tampa Interdisciplinary Environmental Research (TIER)
Ariane Ramsundar
1. Bio Sketch
1.1. Home Institution: University of the Virgin Islands
1.2. Major: Applied Mathematics
1.3. Classification: Senior
1.4. USF Research Mentor: Laura Rodriguez-Gonzalez
1.5. Professional Goals: Upon graduation I plan to attend graduate
school to pursue an advanced degree in my field of
engineering.
2. Abstract
Adsorption studies on a Clinoptilolite packed column for treatment of
Septic Tank Effluent
Ariane Ramsundar1, Laura Rodriguez-Gonzalez2, Dr. Sarina Ergas2
1 Civil
and Environmental Engineering, University of the Virgin Islands
of Civil and Environmental Engineering, University of South Florida
2Department
ariane121@msn.com
Keywords: Nitrification, Denitrification, Clinoptilolite, Biological and Physical Processes
The main goal of this project was to investigate the effectiveness of clinoptilolite for the
removal of nitrogen from onsite wastewater treatment systems. Other media such as lava rock,
sand, experimental clay, glass, plastic, and vermiculite were tested in adsorption experiments to
determine which one is best at absorbing nitrogen. Onsite wastewater treatment systems have
some issues such as variable loading rates and limited to no nitrogen removal. Excessive nitrogen
loadings in water can cause many effects such as eutrophication in water which can be harmful to
aquatic species and even humans. The goal of this research was centered on enhancing nitrogen
removal by combining two removal processes. These processes included biological, nitrification
and denitrification, and physical, addition of an adsorptive or ion exchange media. Column
experiments were conducted to study the sorption of ammonia from onsite wastewater treatment
systems. A glass column of 40.64 cm height and volume of 0.75L was used. The column was packed
with 24.13 cm in height of clinoptilolite and 5.08 cm in height of sand. The feed of 200 mg NH 4Cl/L
(67 mg NH4+/L) was pumped at a constant flow rate of 4 ml per min. The sampling ports were
spaced evenly over the length of the clinoptilolite and samples were collected every 24 hours from
every port. Based on previous adsorption studies performed on the clinoptilolite, its maximum
adsorption capacity is 27 mg of ammonium per gram of media. It is expected that the first layer of
the column will reach saturation (C=0.9C0) within 5 days and the whole column within 20 days.
61
REU: Tampa Interdisciplinary Environmental Research (TIER)
Chinelo Agbim
1. Bio Sketch
1.1. Home Institution: University of Illinois at
Urbana Champaign
1.2. Major: Civil and Environmental Engineering
1.3. USF Research Mentor: Robert Bair
1.4. Professional Goals: Environmental Protection Agency
2. Abstract
Performance of an anaerobic membrane reactor for the treatment
of food waste
Chinelo Agbim1, Javkhlan Aruinbaatar2, Robert Bair2, Dr. Daniel Yeh2
1Civil
and Environmental Engineering at the University of Illinois at Urbana-Champaign
and Environmental Engineering, University of South Florida, Tampa, FL
agbim2@illinois.edu
2Civil
Keywords: anaerobic membrane bioreactor, food waste, hydraulic retention time
The production and disposal of the organic fraction of municipal solid waste (OFMSW) is a
challenge throughout the world. Food waste (FW) is the single largest fraction of OFMSW, and
represents a potential source of renewable energy and nutrients if managed properly. Anaerobic
digestion (AD) is a promising technology which allows for the sustainable recovery of resources
from FW treatment. Nevertheless, conventional AD requires long hydraulic retention times (HRT)
(~20-40 days) due to slow growing microbes called methanogens. This translates into the
requirement of large capacity digesters which are expensive to build and maintain. This issue is
solved by anaerobic membrane bioreactors (AnMBR) which prevent microbial washout through the
incorporation of a membrane filtration process. This allows for much shorter HRTs and smaller
reactor volumes. The main objective of this study was to evaluate the performance of a lab-scale
AnMBR for the treatment of FW at low HRTs.
A 10 L AnMBR was seeded with anaerobic sludge collected from a local waste water
treatment plant and operated under mesophilic conditions. The reactor was fed with synthetic food
waste diluted to an average chemical oxygen demand (COD) of ~5000 mg/L. The system was
started with a HRT of 20 days, which represents the lower limit for traditional AD systems. The
HRT was subsequently reduced to 5 days. COD and total and volatile solids tests were run on the
feed, reactor, and permeate to evaluate the biological performance of the system. Biogas and
permeate production were continuously monitored using a data acquisition system. The AnMBR
started with an organic loading rate of 0.3g VS/L.day. The bioreactor was stable with a neutral pH
and maintained a COD removal efficiency between 95-98% and volatile solids removal efficiency
between 70-90%. The stability of the reactor and removal efficiency provides the evidence that
AnMBR can effectively treat FW at lower HRTs than traditional AD system.
62
REU: Tampa Interdisciplinary Environmental Research (TIER)
Christopher Roberts
1. Bio Sketch
1.1. Home Institution: University of Technology, Jamaica
1.2. Major: BSc in Civil and Environmental Engineering
1.3. Classification: Freshman
1.4. USF Research Mentor: Karl Payne
1.5. Professional Goals:
2. Abstract
Mobile Technology & Dengue: A water based human centric approach to
beat dengue in the Caribbean
Christopher Roberts1, Karl Payne2, Dr. Maya Trotz2
2Civil
1Civil
Engineering: University of Technology, Jamaica
and Environmental Engineering, University of South Florida
Cer2k6@yahoo.com
Keywords: Dengue, mobile technology, water storage, water management
Dengue is a vector borne disease transmitted by the Aedes aegypti and Aedes albopictus mosquitoes.
Prevalent in the tropics, the Caribbean region is currently facing a serious dengue epidemic with
climate change predictions of increased prevalence owing to changing mosquito gestation periods
and changing water management strategies to deal with increased drought and increased rainfall
intensity. These particular mosquitoes often breed in receptacles like flower pots, gutters,
discarded tires, stagnant drains, and water storage vessels. This research investigates the use of
mobile technology to gather information from the public on dengue incidence and local conditions
(e.g. temperature, rainfall, humidity, elevation, population density, condition of water storage
vessels), raise awareness on dengue and build a cohesive citizen driven network to improve water
management in local communities to eradicate dengue. The initial study site is Barbados, the most
easterly island in the Caribbean with one of the highest number of dengue cases per capita in the
Caribbean. Collaborating with a company that develops mobile surveys, a program was developed
to collect data on dengue cases from smart phone users. This information was integrated into a
website with mapping capability as well as with other social media avenues (twitter, facebook and
instagram) for public engagement and action on the topic. A proposed “beatdengue” campaign was
developed to build on this work and produce local household and community driven action on
improving water management to eradicate breeding sites of the dengue mosquitoes.
63
REU: Tampa Interdisciplinary Environmental Research (TIER)
Clyde E. Joseph
1. Bio Sketch
1.1. Home Institution: University Of South Florida (USF)
1.2. Major: Civil Engineering
1.3. Classification: Senior
1.4. USF Research Mentor: Ryan Locicero
1.5. Professional Goals: Obtain a Ph.D. in Environmental Engineering
and become a leading consultant in the Environmental
Engineering field
2. Abstract
Florida native plants for Rain Gardens- A comparison of effective
phosphorus removal from stormwater runoff
Clyde E. Joseph1, Ryan Locicero2, Maya Trotz2
2Civil
1Civil and Environmental Engineering Department, University of South Florida
and Environmental Engineering Department, University of South Florida
Clydejoseph@mail.usf.edu
Keywords: Rain garden, phosphorous, Stormwater
Phosphorous is a pollutant found in stormwater runoff that can lead to eutrophication of
water bodies. Increased urbanization and population growth in Florida has contributed to a steady
increase of stormwater containing phosphorous and nitrogen, with phosphorous being the limiting
nutrient in freshwater surface bodies. Rain gardens are a type of green infrastructure that retain
stormwater runoff from roofs, impervious surfaces, and vegetated areas so that plants and media
can utilize and remove runoff constituents like phosphorous and enable percolation through the
soil.
Read et al. (2009) suggest that particular plant species combined with a good planting
media mixture accelerate the removal of pollutants from stormwater runoff. The rain garden,
usually ranging from depths of 0.5 to 2.0 feet, serves as a retention area for stormwater. Twelve
Florida native plant species were introduced into and later harvested from three different rain
gardens in Tampa packed with gravel, sand, top soil, and mulch media layers. The plants were
analyzed for phosphorus content using the wet phosphorous technique. These data will be used to
estimate the role of plants in phosphorous removal from a rain garden and compare the
effectiveness
of
the
various
species
to
remove
phosphorous.
64
REU: Tampa Interdisciplinary Environmental Research (TIER)
Donald Dominic Dill
1. Bio Sketch
1.1. Home Institution: Beloit College
1.2. Major: Physics & Applied Chemistry
1.3. Classification: Senior
1.4. USF Research Mentor: Veronica Aponte-Morales
1.5. Professional Goals: Continue education in the field of
environmental engineering. Earn certification as a professional
engineer.
2. Abstract
Zeo-Pack for Nitrogen Removal and Recovery
D. Dominic Dill1, Veronica Aponte-Morales2, Dr. Sarina Ergas2
1Departments
2Department
of Physics & Chemistry, Beloit College
of Civil and Environmental Engineering
dilld@beloit.edu
Keywords: Ammonium removal, nitrification inhibition, ion exchange, chabazite, zeo-pack
Chabazite, a form of zeolite, serves as an ion exchange material and biofilm carrier for the
removal of ammonium from anaerobically digested swine waste. Mixing of solutions containing free
zeolite particles has led to the breakdown of zeolite particles and ultimately a loss of zeolite mass
through decanting effluent. An alternative that may maintain optimal particle size is to enclose the
particles in a “Zeo-Pack,” or a finely meshed Sefar Nitex pouch. This test compares the rates of
nitrification between “Zeo-Pack” enclosed Chabazite reactors with free floating Chabazite reactors.
Six nitrification batch tests with a volume of 600mL and 1000ppm ammonium were evaluated. To
four of the solutions 90g Chabazite was added; two with the “Zeo-Pack” and two without. Two
zeolite free solutions served as controls. Solutions were mixed via a “Jar-Tester” for eight days and
sampled every 24 hours for ammonium, nitrate, and nitrite concentrations. Volatile suspended
solids and alkalinity were also measured and the pH of the solutions was maintained at roughly 7.5
throughout the experiment.
65
REU: Tampa Interdisciplinary Environmental Research (TIER)
Erin Eberhard
1. Bio Sketch
1.1. Home Institution: University of Michigan
1.2. Major: Ecology and Evolutionary Biology
1.3. Classification: Senior
1.4. USF Research Mentor: Javkhlan Ariunbaatar
1.5. Professional Goals: Work for the EPA or DNR
2. Abstract
The effect of using exotic animal dungs as inocula for the anaerobic
digestion of food waste
1Ecology
Erin Eberhard1, Javkhlan Ariunbaatar2, Daniel Yeh2
and Evolutionary Biology, University of Michigan; 2Civil and Environmental Engineering,
University of South Florida
ekeb@umich.edu
Keywords: food waste, biomethane, exotic animal dung
Anaerobic digestion (AD), considered a sustainable method of treating food waste, produces
renewable energy and decreases the environmental impact. Its stable operation is often challenging
and time-consuming. This is particularly true of food waste high percentages of complex organics.
This study investigates different sources of microbial inocula from animal dung and their impact on
the breakdown of complex organics. Animal dungs used were based on diets: herbivorous (giraffe,
llama and koala), carnivorous (tiger), and omnivorous (sloth bear) and were compared with
reported cow, digested sludge, and digested buffalo manure. A series of batch experiments were
carried out on the biomethane potential test of synthetic food waste. Three separate batch
experiments were performed: (i) inoculum with pH buffer, (ii) inoculum without pH buffer, and (iii)
mixture of the best three inocula based on the previous sets. Daily biomethane production was
measured along with lipids, proteins, and carbohydrates at the start and end of the batch tests.
Additional pH buffer resulted in a biomethane production plateau after 18 days. The giraffe dung
had the highest specific biomethane production rate of 161.2 ± 9.96 mLCH4/gVS, followed by sloth
bear dung (133.8 ± 14.71 mLCH4/gVS). Both were higher than commonly reported cow dung (125.4
± 4.58 mLCH4/gVS), but lower than the production rates of 521.5 ± 1.45 mLCH4/gVS and 405.5 ±
9.80 mLCH4/gVS of anaerobic sludge and buffalo manure, respectively. Buffer without inocula
lasted for a week. The tiger dung had the highest specific biomethane production rate of 76.02 ±
1.125 mLCH4/gVS followed by the giraffe dung (59.2 ± 6.67 mLCH4/gVS), which were still lower
than the production of the inocula from the full-scale anaerobic digestion plants that had
biomethane production rates of 399.94 ± 5.39 mLCH4/gVS (anaerobic sludge) and 349.63 ± 12.62
mLCH4/gVS (buffalo manure). This study suggests the source of inocula is crucial for the recovery
of biomethane from food waste, and the dung from exotic animals could be used as inoculum to
enhance biodegradation.
66
REU: Tampa Interdisciplinary Environmental Research (TIER)
Gareth Wyatt
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Secondary Science Education
1.3. Classification: Senior
1.4. USF Research Mentor: Allan Feldman, Ph.D
1.5. Professional Goals: To teach highschool physics,
incorporating the education research skills learnt during
the REU-TIER program to maximize student learning.
2. Abstract
Mentoring Styles in Formal Mentorships During a Summer
Environmental Engineering REU Program
Gareth Wyatt1, Allan Feldman2
1University
2
of South Florida
Department of Secondary Education, College of Education, University of South Florida
gcwyatt@mail.usf.edu
Keywords: Mentoring Styles, undergraduate research, apprenticeship
This study looked at the mentoring styles of graduate student mentors towards
undergraduate mentees during a summer Research Experience for Undergraduates (REU) funded
by the National Science Foundation (NSF). Previous related studies, such as Feldman et al. (2009)
have looked into the role of apprenticeship learning between mentor mentee in the context of
communities of practice.
Using cognitive apprenticeships as a model, this mixed methods study seeks to build on
previous studies and explore the mentoring style of graduate students, seeing how mentors in the
sciences structure learning for their mentees. Qualitative data was collected from the observations
of 14 mentor-mentee dyads and mentor interviews. Quantitative data was collected through
mentor and mentee pre and post surveys.
Preliminary analysis suggest that mentoring styles can be defined as directive and consultative,
depending on whether the mentor assumes full responsibility for mentee learning (directive), or
enables the mentee to direct their own learning (consultative). Furthermore, analysis of
preliminary data suggests that mentor styles can only be described in context by also considering
mentee styles, which are considered to be dependent and independent. The mentoring styles and
the dependency of mentees are seen to adapt over time during the course of the 10 week REU
program.
67
REU: Tampa Interdisciplinary Environmental Research (TIER)
Jabari Lee
1. Bio Sketch
1.1. Home Institution: Florida Gulf Coast University
1.2. Major: Civil and Environmental Engineering
1.3. Classification: Senior
1.4. USF Research Mentor: Ryan Locicero
1.5. Professional Goals: My professional goal is to become an
environmental engineer helping communities improve and
maintain the health of their environment through the use of
environmental remediation and restorations, green
Infrastructure implementation, and sustainable land
management practices.
2. Abstract
Plant Selection Process for Use in Rain Gardens
1Department
2
Jabari Lee1, Ryan Locicero2, Dr. Maya Trotz2
of Civil and Environmental Engineering at Florida Gulf Coast University
Department of Civil and Environmental Engineering at The University of South Florida
jblee5474@eagle.fgcu.edu
Keywords: bioretention, plant selection, nutrient management, plant survivability
Plants are postulated to play a significant role in the long term removal of phosphorous and
nitrogen captured by the soil media in rain gardens through nutrient uptake (Davis et al., 2006).
Currently there is a need to determine which plants are best suited for rain gardens in the
southeastern part of the United States. This research focuses on determining nitrogen uptake and
plant survivability of 12 Florida native plant species to determine which species are best suited for
implementing within rain gardens in the Florida’s Tampa Bay region. The average nitrogen content
(mg N/mg dry weight of plant as a percentage) of the leaves and stems of each plant species was
analyzed using a TN 3000. This was done for plants prior to installation in a rain garden and after
installation. Monitoring and sampling protocols for installed rain gardens were developed and used
at three field sites located on middle and high schools in the Tampa Bay region.
The following four species contained the highest percent nitrogen in the leaves; Spiderwort
(Tradescantia ohiensis) 3.1%, Tropical Sage (Salvia coccinea) 2.9%, Tickseed (Coreopsis
leavenworthii) 2.5%, and Fakahatchee Grass (Tripsacum dactyloides) 2.4%. Of the twelve species
monitored in the field the Tropical Sage survived the most in the three rain gardens. While other
factors like plant availability, cost, and customer preference determine the plants used in rain
gardens, this work contributes to quantitative and qualitative data for assessing the sustainability
and performance of rain gardens.
68
REU: Tampa Interdisciplinary Environmental Research (TIER)
M. Peyton Dilbeck
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Civil Engineering
1.3. Classification: Junior
1.4. USF Research Mentor: Meng Wang
1.5. Professional Goals: To become equipped with both structural
engineering and theological tools to provide physical and
spiritual aid to developing countries in the missionary field.
2. Abstract
Impact of Thermal Pretreatment on Swine-Manure’s Biogas Production in an
Anaerobic Digester
1Civil
M. Peyton Dilbeck1, Meng Wang2, Sarina Ergas2
and Environmental Engineering, University of South Florida; 2Civil and Environmental
Engineering
mpdilbeck@mail.usf.edu
Keywords: swine manure, anaerobic digestion, thermal pretreatment, biological methane potential
Concentrated animal feeding operations (CAFO) produce a surplus of nitrogen and
phosphorous which causes eutrophication. The pathogens in manure also harm public health.
Anaerobic digestion (AD) is a sustainable alternative to stabilize the waste and produce methane
for onsite energy use. This study investigates the impact of thermal pretreatment conditions on
biogas production in an anaerobic digester using swine manure as the primary substrate. Thermal
pretreatment solubilizes organic particulate matter in order to increase the rate-limiting hydrolysis
step of anaerobic digestion and increase biogas yields. Two separate experiments were used to
carry out this study; the first experiment consisted of pretreating swine manure at 70 °C, 90° C and
121°C. In order to determine the pretreatment time, hydrolysis tests were performed at each
temperature with treatment times varying between 20 minutes to 24 hours. A time of 30 minutes
was chosen for 70 °C and 90° C, and 20 minutes for 121°C because it resulted in the highest
increase of hydrolysis efficiency. Once the pretreatment was performed, biological methane
potential (BMP) over the course of a 15-day period was measured in batch reactors.
Un-pretreated swine manure, pretreated swine manure, and effluent from the batch
reactors underwent the following tests : Total Solids (TS) and Volatile Solids (VS) tests to calculate
the volatile solids reduction (VSR), total COD (tCOD) and soluble COD (sCOD) to measure the
solubilized organic particles, Volatile Fatty Acids (VFA) to understand the acetogenic step of AD,
and Total Phosphorous (TP), Total Nitrogen (TN) and Ammonia (NH3 -N) to assess the contents of
the influent and effluent. Biogas production and methane yield were also measured. Increasing
temperature produced a corresponding increase in VFA’s, total and soluble COD, and biogas
production. However, the control had a higher biogas per gram of VS production which could be a
result of no acclimation period for the microbial community in the digesters.
69
REU: Tampa Interdisciplinary Environmental Research (TIER)
Maraida Balaguer-Barbosa
1. Bio Sketch
1.1. Home Institution: University of Puerto Rico at Mayagüez
1.2. Major: Chemical Engineering
1.3. Classification: Senior
1.4. USF Research Mentors: Dr. Nancy Diaz-Elsayed, Pablo
Cornejo-Warner, and Dr. Qiong Zhang
1.5. Professional Goals: Pursue a M.S. in Environmental
Engineering with an emphasis on sewage treatment and
disposal.
2. Abstract
An Evaluation of the Energy and Greenhouse Gas Emissions of Onsite
Wastewater Treatment Systems.
Maraida Balaguer-Barbosa1, Dr. Nancy Diaz-Elsayed2, Pablo Cornejo-Warner2,
and Dr. Qiong Zhang 2
1Chemical
Engineering at University of Puerto Rico at Mayagüez
Engineering at University of South Florida
2Environmental
maraida.balaguer@upr.edu
Keywords: onsite treatment, wastewater, energy
The scope of my research project is to evaluate the energy and greenhouse gas (GHG)
emissions through life cycle assessment (LCA) to assess the environmental aspects and potential
impacts associated with onsite wastewater treatment systems (OWTs). The method consists of (a)
compiling an inventory of relevant energy and materials; (b) evaluates the potential environmental
impacts associated with identified inputs and releases; (c) interpretation of results. A conventional
configuration consisting of a concrete septic tank and a drainfield for discharge was considered as
primary design. A process that includes nitrification and denitrification as advanced treatment was
also considered as the secondary model design.
In this research, the life cycle inventories were obtained through communications with
contractors and manufacturers, a literature search, and inquiries with homeowners and suppliers.
The WESTWeb tool allowed the characterization of potential effects, including energy and green
house gas emissions of these decentralized systems. A functional unit (FU) of 1,000 liters per year
was selected as the unit of output that provides the basis upon which all alternatives are compared.
The comparison between both conventional and nitrification/denitrification scenarios through
WESTWeb toll accomplished the identification of the pump as the greater contributor of energy
consumption during advanced treatment process.
70
REU: Tampa Interdisciplinary Environmental Research (TIER)
Matt Dalrymple
1. Bio Sketch
1.1.Home Institution: Bard College and Columbia University
1.2. Major: Philosophy (Bard) and Civil Engineering (Columbia)
1.3. Classification: Senior
1.4. USF Research Mentor: Dr. Jeffrey Cunningham
1.5. Professional Goals: Use my education to help others gain or maintain
access to those resources which are essential for life
2. Abstract
Degradation of Solid Phase Extraction (SPE) Cartridges
Matt Dalrymple1, Dr. Jeffrey Cunningham2
University, Department of Civil Engineering
2University of South Florida, Department of Civil and Environmental Engineering
1Columbia
mjd2217@columbia.edu
Keywords: Solid-phase extraction, Bisphenol-A, 17ß-Estradiol, C18 Cartridge, HLB Cartridge, Gas
chromatography-mass spectrometry
Solid-phase extraction (SPE) is a method which extracts dissolved chemicals from aqueous
solution so that their concentrations can be quantified by analytical instruments. In order to
perform the SPE method, most researchers rely on a commercially available SPE cartridge. In order
to accommodate a variety of different sample sizes and target analytes, cartridges vary in their size
and composition. Although the cartridges’ manufacturers suggest that the cartridge ought to be
used only once, we will investigate whether or not multiple uses seem to degrade the data yielded
by the cartridge. If SPE cartridges prove to be reusable, they may be employed in situations where
they were previously considered to be not economically viable.
Our study will evaluate the effectiveness of two different SPE cartridges over multiple uses. We
have chosen the following two SPE cartridges for analysis: Oasis® HLB cartridges; and C 18
cartridges. These cartridges have been chosen due to their popular employment when determining
aqueous concentrations of endocrine disrupting compounds, or EDCs. To analyze the effectiveness
of the cartridges over multiple uses, we will run an aqueous solution with known concentrations of
Bisphenol-A (BPA) and 17ß-Estradiol (E2) through three Oasis® HLB cartridges and three C18
cartridges. Using gas chromatography- mass spectrometry to analyze the BPA and E2 concentrated
by the cartridges we will be able to realize the success of the SPE cartridges. By repeating the SPE
process while maintaining the same known concentration of BPA and E2, and also reusing the SPE
cartridges, we will be able to consider whether or not the cartridges are degrading with use.
Comparing data yielded from cartridges which were used only one time (our initial data set) to data
yielded from cartridges used multiple times, will allow us to analyze apparent differences in the
data created by the cartridges’ reuse. If 1) data collected from cartridges which have been used
multiple times differs from the initial data set by an amount greater than the difference between
cartridges in the initial data set or 2) all of the cartridges used n times differ from the initial data set
in a consistent way— for instance, all data collected suggests a lower concentration of BPA and
E2—then we will conclude that the SPE cartridges are in fact single use only.
71
REU: Tampa Interdisciplinary Environmental Research (TIER)
Michelle Henderson
1. Bio Sketch
1.1. Home Institution: The City College of New York, CUNY
1.2. Major: Earth and Atmospheric Science
1.3. Classification: Senior
1.4. USF Research Mentor: Dr. Manoj Ram
1.5. Professional Goals:To pursue a Ph.D. in Environmental
Engineering and/or International Public Health
2. Abstract
Graphene Doped Metal Oxide Nanoparticles for Methyl Orange Removal
Michelle Henderson1, Srikanth Gunti2, Dr. Manoj Ram3
1Earth
and Atmospheric Science, The City College of New York.
Department of Mechanical Engineering, University of South Florida
3 Nanotechnology Research and Education Center, University of South Florida
2
mhender00@citymail.cuny.edu
Keywords: nanomaterials, Photocatalysts, Graphene, Graphene Titanium Oxide, Titanium Dioxide,
TiO2, Zinc Oxide, Methyl Orange
Nanomaterial photocatalysts are widely used for environmental remediation. Titanium
Dioxide (TiO2) has been studied to degrade organic and inorganic pollutants and is most effective in
the ultraviolet spectrum (200-380nm). By shifting the extending the bandgap energy of TiO2 into
the visible spectrum range (450-700nm) photocatalytic abilities can be enhanced. These abilities
can also be enhanced by modifying the structure of TiO2 allowing oxidation of a pollutant in the
valence band of TiO2. Enhancements of TiO2 via doping have been proven to enhance the
photocatalytic abilities of TiO2. The goal of this experiment is to observe the photocatalytic effects
of graphene doping on TiO2, and compare it with other graphene doped metal oxides used as
photocatalyst. Graphene Titanium Dioxide (G-TiO2) nanoparticles and nanowires were synthesized
using a sol-gel and a hydrothermal method. The nanosized materials were then coated in petri
dishes. The indicator pollutant methyl orange was concentrated to 20 ppm and a halogen lamp was
focused on top of the dish to simulate ambient sunlight. Quantitative analysis was completed using
UV Spectroscopy and FTIR. Comparative tests of the photodegradation of methyl orange have
revealed that G-TiO2 is a more suitable photocatalyst than its counterparts of G-ZnO and GZnO+TiO2. With the addition of Graphene, TiO2 is a suitable photocatalyst for remediation of
organics in water. Further tests will be conducted to observe the effects of Graphene doping on TiO2
and
Zinc
Oxide
for
the
photodegradation
of
naphthalene.
72
REU: Tampa Interdisciplinary Environmental Research (TIER)
Stephany Paredes Mesa
1. Bio Sketch
1.1. Home Institution: The city College of New York
1.2. Major: Environmental Engineering
1.3. Classification: Junior
1.4. USF Research Mentor: Wen Zhao
1.5. Professional Goals: To pursue a PhD degree.
2. Abstract
Photo-degradation of Dispersed Oil
Stephany Paredes Mesa1, Wen Zhao2, Norma Alcantar2
1Enviornmental
Engineering Department, City College
Engineering Department, USF
2Chemistry
Stephany.paredes.m@gmail.com
Keywords: oil Spill, Remediation, Photodegradation, Biodegradation, Cactus Mucilage, Dispersant,
TiO2, Photocatalyst.
Oil spills are a form of pollution, they affect the ecosystem and marine life. Its clean up and
recovery are problematic and depend on different factors. Since an oil spill is hard to control and it
spreads quickly, actions have to be taken within a few hours. However, the solution to these
accidents in some cases turns out to be even more harmful than the oil spill itself. Thousands of
liters of chemicals have been added to the ocean in order to mitigate it; causing animals to die and
people’s lifestyles to change. Therefore, action for a better and more natural way to treat this type
of situations is needed.
The purpose with this project is to combine degradation techniques, using biodegradation
and photo-degradation to disperse oil. Biodegradation and photo-degradation have shown to be the
most significant degradation mechanisms. Of the two, photo-chemistry is an important factor for
the degradation of large polycyclic aromatic hydrocarbons (PAHs) which are typically resistant to
biodegradation. PAHs makes the oil toxic for living organisms; however when the oil has been
degraded those compounds remain in the water.
This project focuses on mixing a dispersant and a photo-catalyst and testing their effect in the
surface tension of oil in water emulsion. In this case TiO2 is being used as a photo-catalyst, due to its
efficiency and low toxicity. The cactus mucilage (Optunia ficus indica) acts as the dispersant because
it is a biological nontoxic dispersant. The test of the different mixing solutions have shown that TiO 2
affects the efficiency of the cactus mucilage in reducing the surface tension of oil in water emulsion.
With a TiO2 to Cactus mucilage (NE) ratio of 1:10, the ability of the dispersant is seen to not be
affected by the photo-catalyst making its surface tension close to as if only using the biological
dispersant. Thus, this project will open a path to a better way to remediate oil spills as a cheaper
and environmentally friendly solution.
73
REU: Tampa Interdisciplinary Environmental Research (TIER)
Tiffany-To’Quoya Smith
1. Bio Sketch
1.1. Home Institution: University of the Virgin Islands
1.2. Major: Biology
1.3. Classification: Junior
1.4. USF Research Mentor: Suzie Boxman
1.5. Professional Goals: I plan to pursue an MD/PhD at an
accredited U.S. institution.
2. Abstract
Changes in ammonia, nitrite, and nitrate in bench scale marine
aquaponic systems
Tiffany-To’Quoya Smith1, Suzanne Boxman2, Dr. Maya Trotz2
1 University
of the Virgin Islands College of Science and Mathematics
of Civil and Environmental Engineering
2Department of Civil and Environmental Engineering
2Department
toquoya101@gmail.com
Keywords: aquaponics, aquaculture, hydroponics, sustainability
Aquaponics integrates hydroponics and aquaculture, where fish and plants live and share a
symbiotic relationship. The byproduct of the fish serves as fertilizer to the plants. Aquaculture
consists of the production of fish that can simply be used for home décor, market sale, or personal
consumption. Hydroponics consists of growing plants in water for the same reasons.
The goal of the projects is to maximize economic sustainability and feasibility of food
production through marine aquaponics systems. Small- scale systems were constructed at the Mote
Marine Laboratory in Sarasota, FL, based on fresh-water aquaponics systems constructed at the
University of the Virgin Islands. The fish used in these systems are the Florida native red drum.
Florida native species are ideal because they are valuable and marketable. The sea vegetables, salt
wort and sea purselane are of great quality when considering the goal of aquaponics. It has been
reported that sea purselane provides high nutrient content. As a result, sea purselane , salt wort ,
and the red drum provide excellent sources of food.
To maximize the marketing of the marine species, the chemical composition of the system
should be maintained to ensure plant growth and fish survival. We tested ammonia (NH 3-N),
nitrate (N03--N), and nitrite (NO2--N) as part of the maintenance of the systems. These water quality
tests indicate how well the system works. If ammonia and nitrate levels are too high, this indicates
that the plants are not absorbing these nutrients and as a result, the high concentration becomes
too toxic for fish to survive. A balance between the plant absorbance and filtration in these systems
is needed for efficient nitrate removal and plant growth.
74
REU: Tampa Interdisciplinary Environmental Research (TIER)
Cove Capodice
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Civil Engineering
1.3. Classification: Junior
1.4. USF Research Mentor: Suzie Boxman
1.5. Professional Goals: Something of the equvelent to LHC
2. Abstract
Absorption of Ammonium In Two Biofilter Medias
Cove Capodice1, Suzie Boxman2, Maya Trotz2
1Civil
Engineering University of South Florida
and Environmental Engineering
2Civil
Cove44@gmail.com
Keywords: Aquaponic, Ammonium, Absorption, media, Peat, Expanded clay
Please print your abstract here, Length must be between 0.5 and one page. Use paragraphs as
necessary. Keep the length to one page including all text, figures and equations.
Ammonium is an ionized by product of fish waste and is fatal to fish at low concentrations.
Media can function as a biofilter to facilitate ammonium removal thereby preventing the death of
fish. Biofiltration is an integral part of the artificial ecosystem of aquaponic systems. The serial
method was utilized to examine the absorption of ammonium by two different medias: peat moss
and Hydroton. The results indicated that over 60 minutes the ammonium concentration increased.
The flasks containing peat moss showed higher ammonium concentrations than the flasks with
expanded clay. It is possible that the organic composition of peat moss contributed to the higher
ammonium concentration than the more inert expanded clay.
75
USF REU Site, Globalization and Community Health Field School: Combining Social Science and Engineering
Roberto Infante Rosado
1. Bio Sketch
1.1. Home Institution: University of South Florida
1.2. Major: Chemical Engineering
1.3. Classification: Senior
1.4. USF Research Mentor: Maureen Kinyua
1.5. Professional Goals: He hopes to graduate with a
degree in chemical engineering, and to travel
abroad for his masters degree.
2. Abstract
An Overview of Animal Waste Water management: Biodigesters in the
Monteverde Zone of Costa Rica
Roberto Infante1, Chelsea Solomon2, Yanelis Hernandez3, Melissa Ortiz3, Maureen Kinyua4,
Sarina Ergas4
1USF
Department of Chemical Engineering; 2North Carolina Agricultural and Technical State
University Department of Biomedical Engineering; 3USF Department of Anthropology; 4USF
Department of Environmental Engineering
Robertoi@mail.usf.edu
Keywords: biodigesters, Monteverde, Polyethylene Tubular Digesters, Biogas, manure
There has been an increase in environmental awareness since Costa Rica promised to be
carbon neutral by 2021. Governmental organizations such as a high school in Santa Elena, and The
Earth University have taken it upon themselves to fulfill this initiative through the implementation
of more environmentally conscious technologies such as anaerobic digesters. Anaerobic digestion is
a series of biological processes in which microorganisms break down biodegradable material in the
absence of oxygen. As part of the Globalization and Community health field school REU program a
technical report was developed for a high school in Santa Elena, in the Monteverde region of Costa
Rica to assist the school in better understanding how the biodigesters perform when treating
livestock wastewater if one was implemented on their campus. Installation and proper use of an
anaerobic digester on their campus would assist them in tracking their progress towards carbon
neutrality. Currently the school is using an anaerobic lagoon to treat their swine waste. Tubular
digesters installed by Earth University in the surrounding region were used to provide suggestions
on performance of their biodigesters once they installed one at the school. Influent and effluent
samples were collected and analyzed from 4 tubular digesters treating cow and swine waste. BOD5,
pH and total and volatile solids were analyzed. Soils fertilized with effluent and those without
fertilizer were also analyzed for nutrient content. The school’s surrounding water supply was also
analyzed in order to understand if there is an environmental impact from the school before the
biodigester is implemented. The school will experience environmental benefits from switching
from an anaerobic lagoon to a biodigester through biogas collection. Implementation of a
biodigester will offer the school monetary gains through energy production while decreasing
greenhouse gas emissions from the anaerobic lagoon.
76
REU: College of Engineering/Florida Georgia Louis Stokes Alliance for Minority Participation
Melinda Moffitt
1. Bio Sketch
1.1. Home Institution: University of North Florida
1.2. Major: Biomedical Science and Research
1.3. Classification: Junior
1.4. USF Research Mentor: Nicole Febles, Dr. Frisina and
Dr. Gallant
Professional Goals: Stem Cell Research Scientist
2. Abstract
Effect of Biomaterial Stiffness on Feeder Cell Growth
Moffitt, Melinda1-2, Febles, Nicole3-4,6, Frisina, Robert Ph.D.3-4,
and Gallant, Nathan Ph.D.3,,5-6
1Department
of Biology, University of North Florida
State College at Jacksonville
3Department of Chemical and Biomedical Engineering Department
4Global Center for Speech and Hearing Research
5Department of Mechanical Engineering
6Cellular Mechanotransduction and Biomaterials Laboratory
2Florida
melindaam1183@yahoo.com
Keywords: biomaterials, mouse embryonic fibroblasts, primary cells, feeder cells, cell lineage, stem
cell differentiation
Embryonic stem cells are the precursor to any type of tissue, which makes them invaluable for
potential treatment for many debilitating diseases and disorders. Mouse Embryonic Fibroblasts
(MEFs) are generally utilized as feeder cells to maintain and expand embryonic stem (ES) cell
colonies, by producing growth factors that help the ES cells grow without differentiating into a
specific lineage. Therefore, by optimizing the growth of MEFs, stem cell research can be accelerated.
Biomaterials can be used to mimic attributes of cell environments that can modulate cell behavior,
however, currently, there is limited research on the interactions of primary MEFs with various
biomaterials in culture. Based on recent evidence that matrix stiffness regulates stem cell
differentiation, we have hypothesized that culture surface stiffness will also modulate feeder cell
functions including growth. We investigated the effect of matrix stiffness on MEF growth by varying
the crosslink density in a silicone polymer and tracking cell proliferation over 7 days. This research
will support a larger project on using acoustic stimulation to differentiate ES cells into inner ear
hair cells that are central to hearing. The long term goal is to replace or regenerate this tissue to
restore hearing in individuals with undeveloped or damaged hair cells.
77
NSF REU in Applied Physics at USF is supported by NSF Award DMS #1263066
Program Director: Dr. Sarath Witanachchi
NSF REU Site: Tampa Interdisciplinary Research (TIER) at USF is supported by NSF Award EEC
#11569
Program Directors: Dr. Maya A. Trotz, Dr. Sarina Ergas
NSF REU site: Chemistry Summer Enhanced Experience Discovering Science,
Chem-SEEDS at USF is supported by NSF Award CHE #1156853
Program Directors: Dr. Santiago Sandi-Urena, Patricia Muisener
NSF REU site: REU Site on Ubiquitous Sensing is at USF supported by NSF Award
CNS #1062160
Program Directors: Dr. Miguel Labrador, Dr. Rafael A. Perez
NSF RET in Engineering and Computer Science Site: Water Awareness Research & Education at
USF is supported by NSF Award EEC #1200682
Program Directors: Dr. Maya Trotz and Dr. Yiping Lou
NSF RET: Functional Materials Research Institute is supported by NSF Award EEC #1301054
Program Directors: Dr. Venkat Bhethanabotla and Dr. Sylvia Thomas
College of Engineering REU/Florida Georgia Louis Stokes Alliance for Minority Participation is
supported by NSF Awards DMR #1056475 and HRD #1201981
Program Directors: Dr. Nathan Gallant and Dr. Ralph Turner
Organizing Committee
Mr. Bernard Batson
Dr. Sarath Witanachchi
Ms. Kristen Stojak Repa
Mr. Idalides Vergara
Dr. Miguel Laborador
Dr. Santiago Sandi-Urena
Dr. Maya A. Trotz
Dr. Scott Campbell
Acknowledgements
Cristine Prouty, Domingo Feliciano, Daniel Denmark, Idalides Vergara,
Kristen Stojak Repa, Ryan Locicero, Parker Huhta