Online Resource 1 Table: Bacterial strains and plasmids
Strain or plasmid
Escherichia coli TOP10
Gluconacetobacter xylinus
AY201
pBR325
pBR325+ΔacsD
acsDdm
pCTP1
USER fusion system
pCTP1+ acsDNGFP
pCTP1+ acsDCGFP
acsDNGFP
acsDCGFP
Relevant characteristics
hsdR; mcrA; lacZΔM15; cloning
strain
Derivative of Gluconacetobacter
xylinus ATCC 23769 and
developed as a cellulose overproducing isolate in this lab
Ampr; Tetr; Cmr; cloning vector
BclI-HindIII ΔacsD fragment
gene insert isolated from G.
xylinus strain AY201
Disruption mutant created by
inserting acsD fragment within
the acsD gene region of the wild
type G. xylinus AY201
Cmr; fusion/cargo vector
Fusion system used to construct
complements with GFP tags at
either the N terminus or C
terminus of acsD
Cmr; replicating vector for rescue
acsDNGFP, mobilizable by
electroporation into acsDdm
Cmr; replicating vector for rescue
acsDCGFP, mobilizable by
electroporation into acsDdm
N terminus; Tac::GFP::acsD
C terminus; Tac::acsD::GFP
Source or reference
Invitrogen, Cat # C4040-10
Laboratory Stock
Lab-life, vector database
This study
Laboratory Stock
NEB, cat #M5505
This study
This study
This study
This study