ORYZA OIL & FAT CHEMICAL CO., LTD.
L U T E I N
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LUTEIN-P80
LUTEIN-P40
LUTEIN-L8
ver.  2.0TS

LUTEIN CATALOG ver.2.0 TS
L U T E I N
Health Food Ingredients
for human eyes
IT (Information Technology) has become a popular term everywhere.
However, in recent years, children in elementary school to teenagers in junior high school classes are straining their eyes by using electronic games, cellular phones and personal computers for the Internet.
Deterioration of the visual acuity is advancing especially in junior high school and high school students.
As the relationships between the roles of lutein and zeaxanthin and age-related macular degeneration (AMD), which is the primary cause of blindness, have been increasingly indicated by recent studies, more and more people have begun to incorporate these carotinoids, which are antioxidant nutrients, into their diet.
The product introduced here is a health-promoting dietary supplement, rich in lutein and zeaxanthin, extracted from marigold.
Carotinoids are a group of dyes widely distributed in animals and plants. Lutein is a typical xanthophyll in carotinoids. Zeaxanthin is a carotinoid that is always found with lutein.
Carotinoids have strong activities to eliminate active oxygen and are useful for the prevention of lifestyle-related disorders.
Furthermore, studies in the United States have shown that only lutein and zeaxanthin are present in the eye among the several hundred carotinoids that are known today.
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LUTEIN CATALOG ver.2.0 TS
1 ） Structures
OH
HO
Lutein
OH
HO
2 ） Molecular formula
Zeaxanthin
LUTEIN
ZEAXANTHIN
Molecular formula
Ｃ
40
Ｈ
56
Ｏ
2
Ｃ
40
Ｈ
56
Ｏ
2
Prevention of AMD and cataract
Molecular weight
568.88
568.88
An extraocular muscle
Melting point
190 ℃
207 ℃
Zinn’s zonule
Lens
Cornea
Pupil
Ciliary body
Iris
Vitreum
Retina
Optic nerve
Macula: where Lutein and
Zeaxanthin are present
Fig.1. Structure of eye
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LUTEIN CATALOG ver.2.0 TS
Cataracts and retinal detachment are disorders of the eye associated with aging, but agerelated macular degeneration (AMD) is increasing in recent years.
This disorder occurs first in one eye, progresses slowly without pain, extends to both eyes in time, and causes blindness in the worst case.
As no treatment, surgical or chemical, effective for this condition has been developed to date, many studies of its prevention or measures to delay of its progression are in progress.
AMD is considered to occur due to oxidative degeneration of cells in the macular region by active oxygen, which increases as lutein decreases in the macular region with aging.
In cataracts, the visual acuity is reduced as the lens becomes opaque, and this opacity is also caused by active oxygen.
Thus, decreases in lutein and zeaxanthin are considered to be related to aging of the eye.
It has also been discovered by Dr.Snoddary of Harvard University that lutein absorbs blue light, which is likely to damage the eye.
In the same manner that triglycerides and fats are hydrolyzed in the digestive tract to the corresponding fatty acids in order to be absorbed, lutein esters are also hydrolyzed to fatty acids liberating free lutein, which is then absorbed into the blood serum.
Certain enzymes known as esterases and lipases and perform hydrolysis of esters in digestive track.
These enzymes are produced in the pancreas by a mechanism that is regulated by the presence of fat in the stomach and duodenum, so as in the case of free lutein, the bioavailability of lutein Esters is also enhanced by the fat in foods (Roodenburg et al. 2000).
Lutein Ester    Free Lutein     Free Lutein In Serum
These are studies on the bioavability of lutein esters that show a good incorporation of serum when lutein esters are supplemented.
The following graph shows the lutein assimilation in blood plasma,or bioavaility, obtained in these studies.
The curve in short broken line corresponds to the results reported by Berendschot where eight subjects took a daily dose of 10 mg of (as lutein esters) for a period of 84 days
(Berendschot et al. 2000).
The curve in long broken line corresponds to Granado’s study where subject supplemented their diets with 15 mg of lutein (in the form of lutein esters) per day for a period of 112 days
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LUTEIN CATALOG ver.2.0 TS
(Granado et al. 1998)
The line curve corresponds to Landrum’s study where 2 subjects supplemented their diets with 30 mg of lutein (in the form of lutein esters) per day for a period of 140 days (Landrum et al. 1997)
Metabolism and Absorbed of Lutein Ester
30mg/day Supplementation of Lutein Ester
15mg/day Supplementation of Lutein Ester
10mg/day Supplementation of Lutein Ester   stop
3
2.5
2
1.5
stop stop
1
0.5
start
0
0 20 40 60 80 100 120 140 160 180 200
Days
Berendschot et al., LOVS 41 (2000) 3322 - 3326
Granado et al., Br J Nutr 80 (1998) 445 - 449
Landrum et al., Exp. Eye Res. 65 (1997) 57 - 62
From these reports it is possible to summarize the following
：
1) Lutein ester is a good source of lutein since both studies show that high levels of lutein in blood plasma are obtained in relatively short times (7-10 days).
2) The concentration of lutein in plasma depends on the amount of lutein ester supplemented
．
3) Lutein concentration remains at its highest level as long as supplementation is maintained
．
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LUTEIN CATALOG ver.2.0 TS
4)nce supplementation stop ， basal levels of lutein are reached in a period 15 to 20 days ．
Free lutein from the lutein diester formulation appeared in the peripheral circulation more quickly than the unesterified lutein formulation, and attained a higher peak concentration. The mean AUC (Areas under the serum lutein concentration time curves from 0 to 408h) of lutein diester formulation was 61.6% greater than that of unesterified lutein formulation.
Mean peak concentration for both lutein formulations was achieved at 32 h with considerable variation among subjects. At this higher peak concentration, absorption of free lutein from the lutein diester formulation is two times greater than that of unesterified lutein formulation
(Fig.3.).
In the paired baseline adjusted AUC comparisons of the two lutein formulation for each individual, apparent absorption of free lutein from the lutein diester formulation was greater in
14 of the 18 subjects (Fig.4.).
These data show that bioavailability of the lutein diester formulation is greater than that of unesterified lutein formulation.
* P=0.03
Lutein diester
Lutein
Time(h)
Phylips E. Bowen et al., J. Nutr. 132 : 3668 - 3673, 2002
Fig.3. Lutein and Lutein Ester Bioavailability
(Serum lutein concentration)

LUTEIN CATALOG ver.2.0 TS
□  Lutein Diester
■ Lutein
Subject Number
Phylips E. Bowen et al., J. Nutr. 132 : 3668 - 3673, 2002
Fig.4. Lutein and Lutein Ester Bioavailability(AUC)
Two paper were referred to have studied the bioavailability of lutein ester (Landrum et al.
1997, and Berendschot et al. 2000). In both paper, not only the levels of lutein in serum reached by the supplementation of lutein ester were studied, but also how this lutein in serum was deposited in the macula. For example in Landrum’s paper using a heterochromatic flicker photometer for macular pigment optical density measurements it was found 50% increase in optical density as shown in the following graph.
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LUTEIN CATALOG ver.2.0 TS
3
2.5
2
30mg/day Supplementation of
Lutein Ester  for 140 days
0.90
1.5
1
Optical density
0.5
0
0.60
200
0.55
40 80
Days
120 160
Landrum et al., Exp.Eye Res. 65 (1997) 57 - 62
Fig.5. Absorbed of Lutein Ester and Lutein Ester Deposit in
Mocular Pigment
Berendschot reports similar evidence to Landrum’s report by using a lower than 30mg/day supplementation of lutein Ester and using scanning laser ophthalmoscopy and reflectance spectral analysis for measuring the macular pigment optical density.
These two paper prove that lutein ester not only is well absorbed into the human body, but also that the lutein provided by lutein ester supplementation is well deposited into the macular pigment.
Spinach contains a high level of lutein. Although broccoli, Brussels sprouts, and green beans also contain lutein, spinach is considered to be the best food in terms of the ease and efficiency of lutein intake.
A recommended daily lutein intake for prevention of AMD is reported to be 6 mg/day (as free lutein).
To eat this quantity of lutein from spinach alone, one must eat 5 salad bowls of spinach
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LUTEIN CATALOG ver.2.0 TS according to an estimation in the United States. This is impractical.
This lutein intake may be easily achieved by the use of a supplement.
The product introduced here has a very high lutein content, which is 80% as lutein ester
(40% as free lutein). An intake of 15-20 mg/day is recommended particularly for prevention of AMD.
The change in content of lutein ester dose not occur at room temperature and original
Packing. Data comes from the study of LUTEIN-P80 kept in original packing and room temperature.
120
100
80
60
40
20
0
0 150 180 30 60 90
Days
120
Fig.6. Longtime Stability of Lutein Ester
11-1.
Assayed Items
BHC
DDT
Aldrin
Dieldrin
Endrin
Diazinon
Parathion
Results
Not Detected
Not Detected
Not Detected
Not Detected
Not Detected
Not Detected
Not Detected
Detection Limits
0.02ppm
0.02ppm
0.01ppm
0.01ppm
0.01ppm
0.05ppm
0.05ppm
Assay Method
Gas Chromatography
Gas Chromatography
Gas Chromatography
Gas Chromatography
Gas Chromatography
Gas Chromatography
Gas Chromatography
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LUTEIN CATALOG ver.2.0 TS
Test trustee : Japan Food Research Center Foundation
Date of issue of the result report: January 24,2003.
Research issue number : 302120606-001
Five weeks old mice were orally given LUTEIN-P80 (5000mg/kg) and then fed a laboratory chow for two weeks. No toxic effect were observed, thus the LD50 (mouse) is more than
5000mg/kg
Applications
Snacks
Fermentative
Foods
Examples
Dried Foods
Soup, Dried noodles, Seasoning, Pasta, Cereal, Oatmeal, and Topping for pizza
Confectionery Candies, Gum, Cookies, Pudding, Jelly, Yogurt, Chocolate
Rice crackers, Cookies, and Wafers
Bread and Yogurt
Drinks
Others
Protein Shakes , Nutritional Drinks
Health foods, Nutraceutical foods, and Functional foods
LUTEIN -P80
(powder)
1kg
Interior packaging
： aluminium-coated plastic bag
Exterior packaging
： cardboard box
LUTEIN -P40   (powder)
1kg
Interior packaging
： aluminium-coated plastic bag
Exterior packaging ： cardboard box
LUTEIN-L8 (water soluble liquid type)
5 kg Interior packaging
： cubic polyetylene container
Exterior packaging
： cardboard can
Store in cool, dry place.
Avoid humidity.
Marigold Extract
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LUTEIN CATALOG ver.2.0 TS
Lutein Ester
※  Please refer to your nation’s standards.
PRODUCT STANDARD
PRODUCT NAME
(FOOD)
This product is extracted and refined from marigold, the flower of Tagetes erecta (Compositae).
It includes more than 80.0 % of lutein esters.
Appearance
Content of Lutein (free)
Content of Lutein Esters
It is yellow orange powder with slightly unique smell.
Min. 40.0 %
(HPLC)
Min. 80.0 % (Content of Lutein (free) 2)
Content of Total Carotenoid (free)
Content of Total Carotenoid Esters
Min. 42.5 %      (UV)
Min. 85.0 % (Content of Total Carotenoid (free) 2)
Loss on Drying   Max. 5.0 % (1g, 40 ℃ Reduced Pressure,
Posphprus Pentaoxide, 4h)
Purity Test
(1) Heavy Metals
Moulds and Yeasts
Max. 10 ppm
(The Japanese Standards for Food Additives)
(2) Arsenic
Max. 1
ppm (Standard Methods of Analysis in Food Safety
Regulation)
Standard Plate Counts
Max. 1 10 3 cfu/g
(Analysis for Hygienic Chemists)
Max. 1 10 2 cfu/g
(Analysis for Hygienic Chemists)
Coliforms
Negative
(Analysis for Hygienic Chemists)
Composition
Ingredients
Marigold extract
Natural tocopherols
Contents
99.75 %
0.25 %
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LUTEIN CATALOG ver.2.0 TS
Total 100.00 %
PRODUCT STANDARD
PRODUCT NAME
(FOOD)
This product is extracted and refined from marigold, the flower of Tagetes erecta (Compositae).
It includes more than 40.0 % of lutein esters.
Appearance
Content of Lutein (free)
Content of Lutein Esters
It is yellow orange powder and has slightly unique smell.
Min. 20.0 %
Min. 40.0 %
(HPLC)
(Content of Lutein (free) 2)
Content of Total Carotenoid (free)
Content of Total Carotenoid Esters
Loss on Drying
Min. 21.0 % (UV)
Min. 42.0 % (Content of Total Carotenoid (free) 2)
Max. 5.0 % (1g, 40 ℃ Reduced Pressure,
Posphprus Pentaoxide, 4h)
Purity Test
(1) Heavy Metals
(2) Arsenic   Max. 1   ppm (Standard Methods of Analysis in Food Safety
Regulation)
Standard Plate Counts   Max. 1 10 3 cfu/g     (Analysis for Hygienic Chemists)
Moulds and Yeasts
Max. 10 ppm     (The Japanese Standards for Food Additives)
Max. 1 10 2 cfu/g (Analysis for Hygienic Chemists)
Coliforms   Negative
Composition
Ingredients
Marigold extract
Natural tocopherols
Total
(Analysis for Hygienic Chemists)
Contents
99.75 %
0.25 %
100.00 %
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LUTEIN CATALOG ver.2.0 TS
PRODUCT STANDARD
PRODUCT NAME
(FOOD)
This product is emulsifing liquid extracted and refined from marigold, the flower of Tagetes erecta
(Compositae). It includes more than 8.0 % of lutein esters.
Appearance It is orange liquid and has slightly unique smell.
Content of Lutein (free) Min. 4.0 %
(HPLC )
Content of Lutein Esters Min. 8.0 % (Content of Lutein (free) 2)
Purity Test
(1) Heavy Metals Max. 10 ppm
(The Japanese Standards for Food Additives)
(2) Arsenic Max. 1 ppm
(Standard Methods of Analysis in Food Safety
Regulation)
Standard Plate Counts Max. 1 10 3 cfu/g
(Analysis for Hygienic Chemists)
Moulds and Yeasts Max. 1 10 2 cfu/g
(Analysis for Hygienic Chemists)
Coliforms Negative
(Analysis for Hygienic Chemists)
Composition
Ingredients
Marigold extract
Hydrogenated glucose syrup
Caprylic/capric acid triglyceride
Glycerin ester of fatty acid
Lecithin
Purified water
Total
Contents
10.0 %
40.0 %
12.4 %
8.0 %
1.6 %
28.0 %
100.0 %
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LUTEIN CATALOG ver.2.0 TS
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